Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64872.t1 | XP_003612345.1 | 97.9 | 755 | 16 | 0 | 1 | 755 | 1 | 755 | 0.00E+00 | 1532.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64872.t1 | P24095 | 74.5 | 757 | 186 | 4 | 1 | 755 | 1 | 752 | 0.0e+00 | 1177.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64872.t1 | G7K036 | 97.9 | 755 | 16 | 0 | 1 | 755 | 1 | 755 | 0.0e+00 | 1532.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049633 | MS.gene64872 | -0.811866 | 5.65E-51 | -1.69E-46 |
| MS.gene049634 | MS.gene64872 | 0.801156 | 1.03E-48 | -1.69E-46 |
| MS.gene050096 | MS.gene64872 | 0.808485 | 3.03E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene64872 | -0.80351 | 3.37E-49 | -1.69E-46 |
| MS.gene050165 | MS.gene64872 | -0.805489 | 1.30E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene64872 | 0.801067 | 1.07E-48 | -1.69E-46 |
| MS.gene050504 | MS.gene64872 | -0.803192 | 3.92E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene64872 | -0.820006 | 8.62E-53 | -1.69E-46 |
| MS.gene05079 | MS.gene64872 | 0.800209 | 1.60E-48 | -1.69E-46 |
| MS.gene050809 | MS.gene64872 | 0.807936 | 3.96E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene64872 | -0.809794 | 1.59E-50 | -1.69E-46 |
| MS.gene050871 | MS.gene64872 | -0.806769 | 7.00E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene64872 | 0.834474 | 2.94E-56 | -1.69E-46 |
| MS.gene051138 | MS.gene64872 | 0.839403 | 1.62E-57 | -1.69E-46 |
| MS.gene051651 | MS.gene64872 | 0.802079 | 6.65E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene64872 | -0.804841 | 1.78E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene64872 | -0.804535 | 2.06E-49 | -1.69E-46 |
| MS.gene051874 | MS.gene64872 | -0.803251 | 3.81E-49 | -1.69E-46 |
| MS.gene051907 | MS.gene64872 | 0.814899 | 1.22E-51 | -1.69E-46 |
| MS.gene05244 | MS.gene64872 | 0.80708 | 6.02E-50 | -1.69E-46 |
| MS.gene052872 | MS.gene64872 | -0.802033 | 6.80E-49 | -1.69E-46 |
| MS.gene053031 | MS.gene64872 | 0.800941 | 1.14E-48 | -1.69E-46 |
| MS.gene053420 | MS.gene64872 | 0.82769 | 1.36E-54 | -1.69E-46 |
| MS.gene054290 | MS.gene64872 | 0.803196 | 3.91E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene64872 | -0.811483 | 6.85E-51 | -1.69E-46 |
| MS.gene054884 | MS.gene64872 | 0.811217 | 7.82E-51 | -1.69E-46 |
| MS.gene054959 | MS.gene64872 | 0.803422 | 3.51E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene64872 | -0.811154 | 8.07E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene64872 | -0.81018 | 1.31E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene64872 | -0.806007 | 1.01E-49 | -1.69E-46 |
| MS.gene056549 | MS.gene64872 | -0.80037 | 1.49E-48 | -1.69E-46 |
| MS.gene056590 | MS.gene64872 | 0.802588 | 5.22E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene64872 | 0.8026 | 5.20E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene64872 | 0.873075 | 1.95E-67 | -1.69E-46 |
| MS.gene058268 | MS.gene64872 | 0.801105 | 1.05E-48 | -1.69E-46 |
| MS.gene058603 | MS.gene64872 | 0.825687 | 4.09E-54 | -1.69E-46 |
| MS.gene058723 | MS.gene64872 | -0.800987 | 1.11E-48 | -1.69E-46 |
| MS.gene058773 | MS.gene64872 | -0.801005 | 1.10E-48 | -1.69E-46 |
| MS.gene059022 | MS.gene64872 | -0.802614 | 5.16E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene64872 | -0.802612 | 5.17E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene64872 | -0.815217 | 1.04E-51 | -1.69E-46 |
| MS.gene059902 | MS.gene64872 | -0.80616 | 9.41E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene64872 | -0.833171 | 6.22E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene64872 | -0.80698 | 6.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64872.t1 | MTR_5g024020 | 98.808 | 755 | 9 | 0 | 1 | 755 | 1 | 755 | 0.0 | 1546 |
| MS.gene64872.t1 | MTR_5g024020 | 94.172 | 755 | 8 | 1 | 1 | 755 | 1 | 719 | 0.0 | 1461 |
| MS.gene64872.t1 | MTR_8g018730 | 83.179 | 755 | 124 | 2 | 1 | 755 | 1 | 752 | 0.0 | 1326 |
| MS.gene64872.t1 | MTR_8g018735 | 83.841 | 755 | 120 | 2 | 1 | 755 | 1 | 753 | 0.0 | 1325 |
| MS.gene64872.t1 | MTR_8g018730 | 79.470 | 755 | 122 | 3 | 1 | 755 | 1 | 722 | 0.0 | 1256 |
| MS.gene64872.t1 | MTR_2g099560 | 71.921 | 755 | 200 | 7 | 3 | 755 | 7 | 751 | 0.0 | 1136 |
| MS.gene64872.t1 | MTR_2g099570 | 69.139 | 755 | 219 | 5 | 3 | 755 | 7 | 749 | 0.0 | 1082 |
| MS.gene64872.t1 | MTR_8g018420 | 68.499 | 746 | 222 | 5 | 12 | 755 | 9 | 743 | 0.0 | 1077 |
| MS.gene64872.t1 | MTR_3g081130 | 68.289 | 760 | 222 | 10 | 1 | 755 | 1 | 746 | 0.0 | 1063 |
| MS.gene64872.t1 | MTR_8g018735 | 82.172 | 617 | 108 | 2 | 1 | 617 | 1 | 615 | 0.0 | 1048 |
| MS.gene64872.t1 | MTR_8g018570 | 64.069 | 757 | 262 | 7 | 3 | 755 | 7 | 757 | 0.0 | 1026 |
| MS.gene64872.t1 | MTR_8g018430 | 64.572 | 748 | 255 | 6 | 10 | 755 | 9 | 748 | 0.0 | 1021 |
| MS.gene64872.t1 | MTR_8g018550 | 65.741 | 756 | 249 | 7 | 3 | 755 | 7 | 755 | 0.0 | 1020 |
| MS.gene64872.t1 | MTR_8g018690 | 63.576 | 755 | 264 | 5 | 3 | 755 | 7 | 752 | 0.0 | 1013 |
| MS.gene64872.t1 | MTR_8g018510 | 64.305 | 748 | 260 | 6 | 11 | 755 | 13 | 756 | 0.0 | 1011 |
| MS.gene64872.t1 | MTR_8g020990 | 63.974 | 755 | 264 | 6 | 3 | 755 | 7 | 755 | 0.0 | 1008 |
| MS.gene64872.t1 | MTR_8g018520 | 63.889 | 756 | 263 | 7 | 3 | 755 | 7 | 755 | 0.0 | 999 |
| MS.gene64872.t1 | MTR_3g081130 | 69.617 | 678 | 197 | 6 | 82 | 755 | 2 | 674 | 0.0 | 994 |
| MS.gene64872.t1 | MTR_8g018690 | 61.457 | 755 | 260 | 6 | 3 | 755 | 7 | 732 | 0.0 | 965 |
| MS.gene64872.t1 | MTR_8g018450 | 59.121 | 751 | 258 | 11 | 10 | 755 | 9 | 715 | 0.0 | 901 |
| MS.gene64872.t1 | MTR_1g083020 | 57.218 | 762 | 287 | 10 | 6 | 755 | 12 | 746 | 0.0 | 880 |
| MS.gene64872.t1 | MTR_8g018730 | 86.837 | 471 | 62 | 0 | 285 | 755 | 2 | 472 | 0.0 | 864 |
| MS.gene64872.t1 | MTR_7g113410 | 55.556 | 774 | 299 | 13 | 1 | 755 | 8 | 755 | 0.0 | 853 |
| MS.gene64872.t1 | MTR_1g104650 | 54.731 | 782 | 312 | 13 | 5 | 770 | 1 | 756 | 0.0 | 851 |
| MS.gene64872.t1 | MTR_8g018650 | 54.618 | 747 | 275 | 12 | 12 | 755 | 9 | 694 | 0.0 | 795 |
| MS.gene64872.t1 | MTR_8g018620 | 54.473 | 749 | 273 | 14 | 12 | 755 | 9 | 694 | 0.0 | 786 |
| MS.gene64872.t1 | MTR_8g018590 | 55.430 | 709 | 265 | 13 | 65 | 755 | 35 | 710 | 0.0 | 764 |
| MS.gene64872.t1 | MTR_8g020970 | 47.034 | 708 | 354 | 12 | 57 | 752 | 113 | 811 | 0.0 | 642 |
| MS.gene64872.t1 | MTR_4g066170 | 43.553 | 760 | 386 | 18 | 4 | 745 | 48 | 782 | 0.0 | 588 |
| MS.gene64872.t1 | MTR_3g479460 | 42.685 | 745 | 387 | 15 | 18 | 745 | 58 | 779 | 0.0 | 587 |
| MS.gene64872.t1 | MTR_8g011340 | 43.508 | 724 | 370 | 17 | 56 | 755 | 46 | 754 | 0.0 | 577 |
| MS.gene64872.t1 | MTR_8g080230 | 43.407 | 728 | 373 | 18 | 50 | 755 | 92 | 802 | 0.0 | 576 |
| MS.gene64872.t1 | MTR_3g079450 | 40.866 | 739 | 398 | 14 | 24 | 745 | 62 | 778 | 0.0 | 560 |
| MS.gene64872.t1 | MTR_4g088810 | 43.485 | 660 | 331 | 14 | 104 | 752 | 151 | 779 | 0.0 | 542 |
| MS.gene64872.t1 | MTR_3g079420 | 45.440 | 647 | 328 | 12 | 113 | 745 | 106 | 741 | 0.0 | 542 |
| MS.gene64872.t1 | MTR_8g020970 | 45.026 | 573 | 294 | 12 | 57 | 617 | 113 | 676 | 7.05e-155 | 467 |
| MS.gene64872.t1 | MTR_8g021020 | 67.021 | 282 | 93 | 0 | 474 | 755 | 37 | 318 | 5.51e-137 | 412 |
| MS.gene64872.t1 | MTR_3g079450 | 37.971 | 611 | 340 | 14 | 24 | 617 | 62 | 650 | 7.82e-128 | 396 |
| MS.gene64872.t1 | MTR_6g452500 | 42.969 | 512 | 264 | 13 | 254 | 754 | 14 | 508 | 9.50e-127 | 393 |
| MS.gene64872.t1 | MTR_7g012030 | 60.684 | 117 | 45 | 1 | 522 | 637 | 87 | 203 | 1.47e-40 | 148 |
| MS.gene64872.t1 | MTR_6g046330 | 54.688 | 128 | 46 | 2 | 522 | 637 | 77 | 204 | 2.52e-35 | 137 |
| MS.gene64872.t1 | MTR_8g463810 | 37.168 | 226 | 75 | 5 | 430 | 653 | 197 | 357 | 3.22e-33 | 133 |
| MS.gene64872.t1 | MTR_5g023990 | 66.176 | 68 | 10 | 1 | 653 | 720 | 145 | 199 | 2.96e-20 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64872.t1 | AT1G55020 | 56.579 | 760 | 286 | 12 | 10 | 755 | 18 | 747 | 0.0 | 858 |
| MS.gene64872.t1 | AT3G22400 | 53.796 | 764 | 309 | 12 | 10 | 755 | 32 | 769 | 0.0 | 819 |
| MS.gene64872.t1 | AT1G17420 | 46.110 | 707 | 359 | 12 | 57 | 752 | 108 | 803 | 0.0 | 620 |
| MS.gene64872.t1 | AT1G72520 | 45.827 | 707 | 362 | 11 | 57 | 752 | 114 | 810 | 0.0 | 618 |
| MS.gene64872.t1 | AT3G45140 | 44.932 | 730 | 361 | 20 | 30 | 745 | 70 | 772 | 0.0 | 582 |
| MS.gene64872.t1 | AT3G45140 | 48.287 | 642 | 303 | 16 | 118 | 745 | 4 | 630 | 0.0 | 575 |
| MS.gene64872.t1 | AT1G67560 | 44.789 | 710 | 353 | 15 | 64 | 753 | 111 | 801 | 0.0 | 561 |
Find 177 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATGCAGCTGTTAATTT+TGG | 0.118332 | 5.3:+13852008 | MS.gene64872:CDS |
| TTTGATTGGGATGCTGATTT+TGG | 0.190953 | 5.3:+13843552 | MS.gene64872:CDS |
| TTGAATTTGTTTGCAATTCA+TGG | 0.215771 | 5.3:+13843670 | MS.gene64872:CDS |
| TTCACCATCTCATCATAATT+TGG | 0.225540 | 5.3:-13852104 | None:intergenic |
| CCACAGTGCCCTTGATCTTT+TGG | 0.235465 | 5.3:-13841491 | None:intergenic |
| CATTTACCATTCACTTTGAT+TGG | 0.244250 | 5.3:+13843538 | MS.gene64872:CDS |
| ATACAAGCCTTTCAAAAGTT+TGG | 0.259729 | 5.3:+13852278 | MS.gene64872:CDS |
| TGGTCGAAAACCAACTAAAA+AGG | 0.263615 | 5.3:+13844111 | MS.gene64872:CDS |
| TCTTGAATATTCTGCATCTT+AGG | 0.264727 | 5.3:-13851942 | None:intergenic |
| GGATCTAGCAACTTGTTAAT+AGG | 0.291816 | 5.3:-13851352 | None:intergenic |
| GAGAGAGGGATGTTAAGTTT+TGG | 0.326208 | 5.3:+13852237 | MS.gene64872:intron |
| ATTTATTGTGTCACGGAAAT+GGG | 0.326994 | 5.3:-13851374 | None:intergenic |
| AGATGTTTGGCATATTTGAT+AGG | 0.330603 | 5.3:+13841460 | None:intergenic |
| CTTATGGAACAGGCACTTAA+TGG | 0.333183 | 5.3:+13850804 | MS.gene64872:intron |
| GGATTTGGAGCTTACATCAT+TGG | 0.341118 | 5.3:-13843736 | None:intergenic |
| TATTTATTGTGTCACGGAAA+TGG | 0.341127 | 5.3:-13851375 | None:intergenic |
| CAGTTGACGGACTAGAGATA+TGG | 0.343179 | 5.3:+13851775 | MS.gene64872:CDS |
| AATTCAACGGATTCACGATT+AGG | 0.343202 | 5.3:-13843654 | None:intergenic |
| TCTTGTCTGTAGAAGACTAA+TGG | 0.350358 | 5.3:-13843920 | None:intergenic |
| CATCCAAATGGTGTTAAGTA+TGG | 0.355834 | 5.3:+13850978 | MS.gene64872:CDS |
| CATCAGCAGCATACAAGAAC+TGG | 0.362677 | 5.3:+13851485 | MS.gene64872:CDS |
| GTTCAATACCACCTTCAAAT+AGG | 0.363395 | 5.3:-13844464 | None:intergenic |
| GCACCATACTTAACACCATT+TGG | 0.363952 | 5.3:-13850981 | None:intergenic |
| ATTTGAAACTTACTTGTAGA+AGG | 0.370231 | 5.3:-13844917 | None:intergenic |
| ACGTTAGATATTGCTGTCTA+TGG | 0.376114 | 5.3:+13850617 | MS.gene64872:CDS |
| TGATAGTACAATTTGGCTAT+TGG | 0.379351 | 5.3:+13851040 | MS.gene64872:CDS |
| TGGATGACTGACGAAGAATT+TGG | 0.388201 | 5.3:+13844855 | MS.gene64872:CDS |
| TCTAGTCCGTCAACTGCATA+AGG | 0.389360 | 5.3:-13851768 | None:intergenic |
| TCGAAGCTATCAAACTCATT+TGG | 0.389705 | 5.3:-13844429 | None:intergenic |
| CAGTCCCTTACCAGTCCTTA+AGG | 0.399613 | 5.3:+13844512 | MS.gene64872:CDS |
| GATCTAGCAACTTGTTAATA+GGG | 0.403902 | 5.3:-13851351 | None:intergenic |
| GACGGTGCTACTGCCTTCTT+AGG | 0.411023 | 5.3:+13841633 | MS.gene64872:CDS |
| TCATCTGACTTCCTTACATA+TGG | 0.412674 | 5.3:+13844339 | MS.gene64872:CDS |
| GATGACATCTCAACAGAATT+CGG | 0.416030 | 5.3:-13851466 | None:intergenic |
| TGTCCTAGTTGCATAAGCTT+TGG | 0.418902 | 5.3:-13850895 | None:intergenic |
| GGCAGCATCAAGAACGTTCC+CGG | 0.421123 | 5.3:-13841586 | None:intergenic |
| TGGAAGGAAGTTGTAGAAAA+AGG | 0.421210 | 5.3:+13851888 | MS.gene64872:CDS |
| ATGTGGTAAACTCAACTCTA+TGG | 0.424161 | 5.3:-13850958 | None:intergenic |
| TAATTTGGAGTTCCTTTCTC+CGG | 0.424665 | 5.3:-13852089 | None:intergenic |
| AAGGTGTTGATAGTACAATT+TGG | 0.435084 | 5.3:+13851033 | MS.gene64872:CDS |
| CTGTTTGTTGCTATGATGAA+TGG | 0.436847 | 5.3:-13851310 | None:intergenic |
| AAGCTTGCTCGTCCAGTCCT+TGG | 0.441085 | 5.3:+13844049 | MS.gene64872:CDS |
| AAGAAGACCACCTTGTTTGA+CGG | 0.441487 | 5.3:-13841556 | None:intergenic |
| ATATGATAATGGAACATGAT+TGG | 0.444515 | 5.3:-13851971 | None:intergenic |
| GTTCCAAGAGATGAAAACTT+TGG | 0.447882 | 5.3:+13844306 | MS.gene64872:CDS |
| ATTTACCATTCACTTTGATT+GGG | 0.449296 | 5.3:+13843539 | MS.gene64872:CDS |
| CAGTGCGGAAAATTTCCTTA+AGG | 0.449942 | 5.3:-13844527 | None:intergenic |
| CTATCAACACCTTGGTCTGC+AGG | 0.450678 | 5.3:-13851023 | None:intergenic |
| TCTTGATGCTGCCGGCAGCT+TGG | 0.450950 | 5.3:+13841596 | MS.gene64872:CDS |
| CTGCCGGCAGCTTGGTCGGC+GGG | 0.451570 | 5.3:+13841604 | MS.gene64872:CDS |
| GCAAATGGCAAGGGATTAGT+TGG | 0.452072 | 5.3:+13843453 | MS.gene64872:intron |
| AATCATGTTCCATTATCATA+TGG | 0.452609 | 5.3:+13851973 | MS.gene64872:CDS |
| GCTCCCAAGGTTGGTAGAGT+GGG | 0.452917 | 5.3:-13843504 | None:intergenic |
| GAACAGCTGGAGATTAATCT+AGG | 0.454212 | 5.3:+13850665 | MS.gene64872:CDS |
| TTCCTTATGTAAAATGCTCC+TGG | 0.457180 | 5.3:-13843579 | None:intergenic |
| TGCATCTTGTCTTGCTCCCA+AGG | 0.467682 | 5.3:-13843517 | None:intergenic |
| GCAAGCTTGACATCTTCATC+TGG | 0.469750 | 5.3:-13844034 | None:intergenic |
| TTTGGAAGAGAGATGGTTGC+TGG | 0.470580 | 5.3:+13844873 | MS.gene64872:CDS |
| CTTGCTCGTCCAGTCCTTGG+AGG | 0.472483 | 5.3:+13844052 | MS.gene64872:CDS |
| GGTGGTCTTCTTGATGCTGC+CGG | 0.474897 | 5.3:+13841567 | MS.gene64872:CDS |
| CTTCTAACCCTTCGAGGGTA+AGG | 0.480584 | 5.3:-13844088 | None:intergenic |
| GATGTTTGGCATATTTGATA+GGG | 0.480663 | 5.3:+13841461 | None:intergenic |
| AATTTCCTTAAGGACTGGTA+AGG | 0.482665 | 5.3:-13844517 | None:intergenic |
| TCTTGTCTTGCTCCCAAGGT+TGG | 0.483250 | 5.3:-13843513 | None:intergenic |
| AGAAGCAGTCCATATGATAA+TGG | 0.483627 | 5.3:-13851982 | None:intergenic |
| TCAAAAGTTTGGAAGTAAGC+TGG | 0.485045 | 5.3:+13852289 | MS.gene64872:CDS |
| CGACCACTTCTAACCCTTCG+AGG | 0.485068 | 5.3:-13844094 | None:intergenic |
| CGAAGATGGATCCCGGAGAA+AGG | 0.485557 | 5.3:+13852077 | MS.gene64872:CDS |
| ACCAGTGGCTCATGAGTTGA+TGG | 0.487070 | 5.3:-13851097 | None:intergenic |
| TACTGGACAACGAAAAGAAT+GGG | 0.494054 | 5.3:+13843970 | MS.gene64872:CDS |
| TACAATTTGGCTATTGGCCA+AGG | 0.495798 | 5.3:+13851046 | MS.gene64872:CDS |
| TGCTCCCAAGGTTGGTAGAG+TGG | 0.496348 | 5.3:-13843505 | None:intergenic |
| CGGAAAATTTCCTTAAGGAC+TGG | 0.497528 | 5.3:-13844522 | None:intergenic |
| GTGGTCTTCTTGATGCTGCC+GGG | 0.498077 | 5.3:+13841568 | MS.gene64872:CDS |
| AATGCTCTAATTAATGCTGA+TGG | 0.498741 | 5.3:+13851415 | MS.gene64872:CDS |
| TATGATGTCTACAATGATCT+TGG | 0.499215 | 5.3:+13844007 | MS.gene64872:CDS |
| GTCCCCACTCTACCAACCTT+GGG | 0.499236 | 5.3:+13843501 | MS.gene64872:CDS |
| AGGTGGGCTCACAGTAGAAA+AGG | 0.500073 | 5.3:+13850685 | MS.gene64872:CDS |
| AATCTAAGAGGAGATGGTAC+TGG | 0.504050 | 5.3:+13843953 | MS.gene64872:CDS |
| TACCTCTTGATAAGATCAGC+TGG | 0.505206 | 5.3:-13851529 | None:intergenic |
| GGATCATAGCCAAAAGATCA+AGG | 0.507959 | 5.3:+13841482 | MS.gene64872:CDS |
| GAAGAATTGCAAAATCTAAG+AGG | 0.508305 | 5.3:+13843941 | MS.gene64872:CDS |
| GTGCTAGACCCTCCAAGGAC+TGG | 0.510390 | 5.3:-13844061 | None:intergenic |
| AATTTGGAGTTCCTTTCTCC+GGG | 0.512509 | 5.3:-13852088 | None:intergenic |
| CGTTGATATTTATTGTGTCA+CGG | 0.512648 | 5.3:-13851381 | None:intergenic |
| AGTAAGCTGGCCAAAATTGA+AGG | 0.513964 | 5.3:+13852302 | MS.gene64872:CDS |
| ATTTACAATTACATGAGCCT+TGG | 0.514004 | 5.3:-13851063 | None:intergenic |
| AGCATCCCAATCAAAGTGAA+TGG | 0.517285 | 5.3:-13843544 | None:intergenic |
| GATGCTGCCGGCAGCTTGGT+CGG | 0.520219 | 5.3:+13841600 | MS.gene64872:CDS |
| CTAGTCCGTCAACTGCATAA+GGG | 0.521062 | 5.3:-13851767 | None:intergenic |
| ATCTCAACAGAATTCGGCCC+AGG | 0.524407 | 5.3:-13851460 | None:intergenic |
| AATGGAGCTGGTGTTTGACT+TGG | 0.524882 | 5.3:-13843902 | None:intergenic |
| TGTCCCCACTCTACCAACCT+TGG | 0.528094 | 5.3:+13843500 | MS.gene64872:CDS |
| CTCATCAGTTGGATAATACA+AGG | 0.529376 | 5.3:-13851832 | None:intergenic |
| AGATACGTTCCGGCCTAAGA+AGG | 0.530698 | 5.3:-13841646 | None:intergenic |
| GTCCGACACTTAGTCGAAGA+TGG | 0.531477 | 5.3:+13852063 | MS.gene64872:CDS |
| TTCAGAATATAACCTCCATA+AGG | 0.532169 | 5.3:-13852038 | None:intergenic |
| GACTACCCTTATGCAGTTGA+CGG | 0.533613 | 5.3:+13851762 | MS.gene64872:CDS |
| CGAAGAATTTGGAAGAGAGA+TGG | 0.533979 | 5.3:+13844866 | MS.gene64872:CDS |
| GTGCTACTGCCTTCTTAGGC+CGG | 0.535421 | 5.3:+13841637 | MS.gene64872:CDS |
| ATGCCAAAGCTTATGCAACT+AGG | 0.537181 | 5.3:+13850892 | MS.gene64872:CDS |
| ACCATCAACTCATGAGCCAC+TGG | 0.540362 | 5.3:+13851096 | MS.gene64872:CDS |
| TTGAGTTTACCACATCCAAA+TGG | 0.542511 | 5.3:+13850966 | MS.gene64872:CDS |
| GGGAACGTTCTTGATGCTGC+CGG | 0.543527 | 5.3:+13841588 | MS.gene64872:CDS |
| AGTTGACGGACTAGAGATAT+GGG | 0.546670 | 5.3:+13851776 | MS.gene64872:CDS |
| TACTTAACACCATTTGGATG+TGG | 0.549004 | 5.3:-13850975 | None:intergenic |
| GACCACTTCTAACCCTTCGA+GGG | 0.549674 | 5.3:-13844093 | None:intergenic |
| TATCCCGCCGACCAAGCTGC+CGG | 0.549835 | 5.3:-13841607 | None:intergenic |
| TTGCAAAATCTAAGAGGAGA+TGG | 0.549962 | 5.3:+13843947 | MS.gene64872:CDS |
| ACACTGAATTACAAACTTGG+TGG | 0.553864 | 5.3:+13851868 | MS.gene64872:CDS |
| AACTCCAAATTATGATGAGA+TGG | 0.555053 | 5.3:+13852100 | MS.gene64872:CDS |
| TTGGTCGGCGGGATAATTGA+CGG | 0.557397 | 5.3:+13841615 | MS.gene64872:CDS |
| TGAATTGCAAACAAATTCAA+CGG | 0.559629 | 5.3:-13843667 | None:intergenic |
| ATGGGATGCTATTAAGTCAT+GGG | 0.562373 | 5.3:+13851794 | MS.gene64872:CDS |
| AGCTGGAGATTAATCTAGGT+GGG | 0.562396 | 5.3:+13850669 | MS.gene64872:CDS |
| TGGCCTTCGTCTTGTGATCG+AGG | 0.568239 | 5.3:+13851740 | MS.gene64872:CDS |
| TTGCTCGTCCAGTCCTTGGA+GGG | 0.571582 | 5.3:+13844053 | MS.gene64872:CDS |
| AGATGACAAACCATCCGTCT+TGG | 0.572064 | 5.3:-13841688 | None:intergenic |
| ATTTCCTTAAGGACTGGTAA+GGG | 0.573930 | 5.3:-13844516 | None:intergenic |
| AAGATTACAGATTTAAACAA+AGG | 0.575473 | 5.3:-13844390 | None:intergenic |
| GCTGCCGGCAGCTTGGTCGG+CGG | 0.581708 | 5.3:+13841603 | MS.gene64872:CDS |
| AAACTCAACTCTATGGCCAC+TGG | 0.582518 | 5.3:-13850951 | None:intergenic |
| TTAACCGCAGAGATACGTTC+CGG | 0.583412 | 5.3:-13841656 | None:intergenic |
| AAGACACTGAATTACAAACT+TGG | 0.585183 | 5.3:+13851865 | MS.gene64872:CDS |
| GCACTCATCCTTACCCTCGA+AGG | 0.587455 | 5.3:+13844080 | MS.gene64872:CDS |
| GCTGTAACGTCCGTCAAACA+AGG | 0.587930 | 5.3:+13841546 | MS.gene64872:CDS |
| TTCTGCATCTTAGGCCACCA+TGG | 0.587934 | 5.3:-13851933 | None:intergenic |
| TATGGGATGCTATTAAGTCA+TGG | 0.588056 | 5.3:+13851793 | MS.gene64872:CDS |
| ATCAGTGCTACCAAGACGGA+TGG | 0.588344 | 5.3:+13841678 | MS.gene64872:CDS |
| ATCATCTTGCCTGCAGACCA+AGG | 0.588962 | 5.3:+13851014 | MS.gene64872:CDS |
| TTTGGACAATATCCTTATGG+AGG | 0.593205 | 5.3:+13852026 | MS.gene64872:CDS |
| CTGTAGAAGACTAATGGAGC+TGG | 0.594388 | 5.3:-13843914 | None:intergenic |
| CGAAGGCCATGTGGAGACGA+TGG | 0.595336 | 5.3:-13851726 | None:intergenic |
| CCTCTGATTACATGTGGTGG+TGG | 0.595383 | 5.3:-13844573 | None:intergenic |
| TAATGCTGATGGAATCATAG+AGG | 0.596258 | 5.3:+13851426 | MS.gene64872:CDS |
| CACTCATCCTTACCCTCGAA+GGG | 0.596982 | 5.3:+13844081 | MS.gene64872:CDS |
| GTACTGGACAACGAAAAGAA+TGG | 0.597403 | 5.3:+13843969 | MS.gene64872:CDS |
| AATACAAGGAGACATAGTCT+TGG | 0.597621 | 5.3:-13851818 | None:intergenic |
| CTTGGAACATACATCACACC+TGG | 0.601033 | 5.3:-13844291 | None:intergenic |
| ATATAGAGGATTAGCAGTTG+AGG | 0.604087 | 5.3:+13851701 | MS.gene64872:intron |
| GATGAGTGCTAGACCCTCCA+AGG | 0.604496 | 5.3:-13844066 | None:intergenic |
| TGAATTACAAACTTGGTGGA+AGG | 0.608098 | 5.3:+13851872 | MS.gene64872:CDS |
| ACTTAGTCGAAGATGGATCC+CGG | 0.609977 | 5.3:+13852070 | MS.gene64872:CDS |
| GATCATAGCCAAAAGATCAA+GGG | 0.615451 | 5.3:+13841483 | MS.gene64872:CDS |
| TTTATTGTGTCACGGAAATG+GGG | 0.616340 | 5.3:-13851373 | None:intergenic |
| TGTAGAAGGCGAATCACACA+AGG | 0.616729 | 5.3:-13844903 | None:intergenic |
| CAGCTGGAGATTAATCTAGG+TGG | 0.616897 | 5.3:+13850668 | MS.gene64872:CDS |
| TACCAGCTGATCTTATCAAG+AGG | 0.619833 | 5.3:+13851527 | MS.gene64872:CDS |
| CGTGACACAATAAATATCAA+CGG | 0.620852 | 5.3:+13851382 | MS.gene64872:CDS |
| TTATAGTTAGCAAATCTGCA+TGG | 0.622128 | 5.3:+13844835 | MS.gene64872:intron |
| CTTGTATTATCCAACTGATG+AGG | 0.626923 | 5.3:+13851833 | MS.gene64872:CDS |
| ATCAGCAGCATACAAGAACT+GGG | 0.628225 | 5.3:+13851486 | MS.gene64872:CDS |
| ATCCATCTTCGACTAAGTGT+CGG | 0.630524 | 5.3:-13852065 | None:intergenic |
| GTTAATCAGTGCTACCAAGA+CGG | 0.631598 | 5.3:+13841674 | MS.gene64872:CDS |
| AAATTGTACTATCAACACCT+TGG | 0.634160 | 5.3:-13851031 | None:intergenic |
| TACCCTCGAAGGGTTAGAAG+TGG | 0.635885 | 5.3:+13844091 | MS.gene64872:CDS |
| CTCCCAAGGTTGGTAGAGTG+GGG | 0.636848 | 5.3:-13843503 | None:intergenic |
| TAGGTTGAATACTCATGCAG+TGG | 0.652665 | 5.3:+13851282 | MS.gene64872:intron |
| ATCACAAGACGAAGGCCATG+TGG | 0.656555 | 5.3:-13851735 | None:intergenic |
| GAGGATCCATCGTCTCCACA+TGG | 0.659512 | 5.3:+13851720 | MS.gene64872:CDS |
| CCACCACCACATGTAATCAG+AGG | 0.659952 | 5.3:+13844573 | MS.gene64872:CDS |
| GAAGTGCGTGACCTATTTGA+AGG | 0.661942 | 5.3:+13844453 | MS.gene64872:CDS |
| TTATGGAACATTGAAACCAG+TGG | 0.684457 | 5.3:+13850935 | MS.gene64872:CDS |
| ATCTTCGACTAAGTGTCGGA+CGG | 0.684688 | 5.3:-13852061 | None:intergenic |
| GTGACTTGAAAGACAAACCA+TGG | 0.687567 | 5.3:+13851916 | MS.gene64872:CDS |
| CCAAAAGATCAAGGGCACTG+TGG | 0.697540 | 5.3:+13841491 | MS.gene64872:CDS |
| GATCCTAAAAGTGAGAGACC+AGG | 0.704866 | 5.3:+13844273 | MS.gene64872:intron |
| GTACCTCTGATTACATGTGG+TGG | 0.712492 | 5.3:-13844576 | None:intergenic |
| AGTCCTCGATCACAAGACGA+AGG | 0.713341 | 5.3:-13851743 | None:intergenic |
| GTAACGTCCGTCAAACAAGG+TGG | 0.717170 | 5.3:+13841549 | MS.gene64872:CDS |
| AATGTACCTCTGATTACATG+TGG | 0.724970 | 5.3:-13844579 | None:intergenic |
| GTGCGTGACCTATTTGAAGG+TGG | 0.729776 | 5.3:+13844456 | MS.gene64872:CDS |
| GGACTTGTTCACCATCAGTG+CGG | 0.739041 | 5.3:-13844542 | None:intergenic |
| TAGGCCGGAACGTATCTCTG+CGG | 0.741507 | 5.3:+13841652 | MS.gene64872:CDS |
| ACTTGAAAGACAAACCATGG+TGG | 0.743773 | 5.3:+13851919 | MS.gene64872:CDS |
| GTAGAAGGCGAATCACACAA+GGG | 0.752151 | 5.3:-13844902 | None:intergenic |
| CATGTGGTGGTGGAAACTTG+AGG | 0.782802 | 5.3:-13844563 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 13841462 | 13852359 | 13841462 | ID=MS.gene64872 |
| chr5.3 | mRNA | 13841462 | 13852359 | 13841462 | ID=MS.gene64872.t1;Parent=MS.gene64872 |
| chr5.3 | exon | 13841462 | 13841699 | 13841462 | ID=MS.gene64872.t1.exon1;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13841462 | 13841699 | 13841462 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13843454 | 13843743 | 13843454 | ID=MS.gene64872.t1.exon2;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13843454 | 13843743 | 13843454 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13843893 | 13844133 | 13843893 | ID=MS.gene64872.t1.exon3;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13843893 | 13844133 | 13843893 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13844274 | 13844594 | 13844274 | ID=MS.gene64872.t1.exon4;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13844274 | 13844594 | 13844274 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13844841 | 13844926 | 13844841 | ID=MS.gene64872.t1.exon5;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13844841 | 13844926 | 13844841 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13850599 | 13850706 | 13850599 | ID=MS.gene64872.t1.exon6;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13850599 | 13850706 | 13850599 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13850816 | 13851117 | 13850816 | ID=MS.gene64872.t1.exon7;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13850816 | 13851117 | 13850816 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13851285 | 13851548 | 13851285 | ID=MS.gene64872.t1.exon8;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13851285 | 13851548 | 13851285 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13851707 | 13852121 | 13851707 | ID=MS.gene64872.t1.exon9;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13851707 | 13852121 | 13851707 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| chr5.3 | exon | 13852240 | 13852359 | 13852240 | ID=MS.gene64872.t1.exon10;Parent=MS.gene64872.t1 |
| chr5.3 | CDS | 13852240 | 13852359 | 13852240 | ID=cds.MS.gene64872.t1;Parent=MS.gene64872.t1 |
| Gene Sequence |
| Protein sequence |
