Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66499.t1 | ADK11431.1 | 98.4 | 375 | 6 | 0 | 1 | 375 | 5 | 379 | 7.80E-212 | 746.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66499.t1 | Q9C9S4 | 60.2 | 372 | 127 | 5 | 8 | 367 | 6 | 368 | 8.7e-119 | 428.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66499.t1 | D9ILX3 | 98.4 | 375 | 6 | 0 | 1 | 375 | 5 | 379 | 5.6e-212 | 746.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049611 | MS.gene66499 | 0.801601 | 8.34E-49 | -1.69E-46 |
| MS.gene050380 | MS.gene66499 | 0.804957 | 1.68E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene66499 | 0.846087 | 2.71E-59 | -1.69E-46 |
| MS.gene050504 | MS.gene66499 | -0.814077 | 1.85E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene66499 | -0.846786 | 1.75E-59 | -1.69E-46 |
| MS.gene050871 | MS.gene66499 | -0.847421 | 1.17E-59 | -1.69E-46 |
| MS.gene050883 | MS.gene66499 | 0.800556 | 1.36E-48 | -1.69E-46 |
| MS.gene05104 | MS.gene66499 | 0.803989 | 2.68E-49 | -1.69E-46 |
| MS.gene051127 | MS.gene66499 | 0.800054 | 1.73E-48 | -1.69E-46 |
| MS.gene051138 | MS.gene66499 | 0.822992 | 1.76E-53 | -1.69E-46 |
| MS.gene05141 | MS.gene66499 | 0.81094 | 8.98E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene66499 | 0.80392 | 2.77E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene66499 | -0.814377 | 1.59E-51 | -1.69E-46 |
| MS.gene052966 | MS.gene66499 | -0.805904 | 1.07E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene66499 | -0.813435 | 2.56E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene66499 | 0.821889 | 3.18E-53 | -1.69E-46 |
| MS.gene053488 | MS.gene66499 | 0.800344 | 1.51E-48 | -1.69E-46 |
| MS.gene054054 | MS.gene66499 | 0.801966 | 7.02E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene66499 | 0.803256 | 3.80E-49 | -1.69E-46 |
| MS.gene055124 | MS.gene66499 | 0.806288 | 8.85E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene66499 | 0.803785 | 2.95E-49 | -1.69E-46 |
| MS.gene055202 | MS.gene66499 | 0.810876 | 9.27E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene66499 | -0.802176 | 6.35E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene66499 | -0.80541 | 1.35E-49 | -1.69E-46 |
| MS.gene056224 | MS.gene66499 | 0.803146 | 4.01E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene66499 | 0.807596 | 4.68E-50 | -1.69E-46 |
| MS.gene056313 | MS.gene66499 | 0.822225 | 2.65E-53 | -1.69E-46 |
| MS.gene056504 | MS.gene66499 | -0.821731 | 3.46E-53 | -1.69E-46 |
| MS.gene057014 | MS.gene66499 | 0.812442 | 4.23E-51 | -1.69E-46 |
| MS.gene058099 | MS.gene66499 | 0.80598 | 1.03E-49 | -1.69E-46 |
| MS.gene058108 | MS.gene66499 | 0.815944 | 7.13E-52 | -1.69E-46 |
| MS.gene058109 | MS.gene66499 | 0.811321 | 7.42E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene66499 | 0.851098 | 1.11E-60 | -1.69E-46 |
| MS.gene058173 | MS.gene66499 | 0.840758 | 7.17E-58 | -1.69E-46 |
| MS.gene058175 | MS.gene66499 | 0.854624 | 1.09E-61 | -1.69E-46 |
| MS.gene058840 | MS.gene66499 | 0.802025 | 6.82E-49 | -1.69E-46 |
| MS.gene059045 | MS.gene66499 | 0.828242 | 1.00E-54 | -1.69E-46 |
| MS.gene059690 | MS.gene66499 | 0.834721 | 2.54E-56 | -1.69E-46 |
| MS.gene059691 | MS.gene66499 | 0.818745 | 1.67E-52 | -1.69E-46 |
| MS.gene059694 | MS.gene66499 | 0.839661 | 1.39E-57 | -1.69E-46 |
| MS.gene059697 | MS.gene66499 | 0.838637 | 2.56E-57 | -1.69E-46 |
| MS.gene059865 | MS.gene66499 | -0.817408 | 3.35E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene66499 | -0.806841 | 6.76E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene66499 | -0.81121 | 7.85E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66499.t1 | MTR_5g063080 | 94.667 | 375 | 20 | 0 | 1 | 375 | 5 | 379 | 0.0 | 743 |
| MS.gene66499.t1 | MTR_3g074180 | 71.676 | 346 | 88 | 4 | 26 | 369 | 24 | 361 | 0.0 | 512 |
| MS.gene66499.t1 | MTR_8g090330 | 65.075 | 335 | 111 | 3 | 40 | 372 | 32 | 362 | 2.13e-156 | 444 |
| MS.gene66499.t1 | MTR_4g101600 | 52.108 | 332 | 137 | 2 | 64 | 373 | 57 | 388 | 2.75e-123 | 361 |
| MS.gene66499.t1 | MTR_4g036090 | 54.573 | 328 | 127 | 2 | 68 | 373 | 59 | 386 | 9.93e-121 | 354 |
| MS.gene66499.t1 | MTR_8g090330 | 64.639 | 263 | 87 | 3 | 40 | 300 | 32 | 290 | 2.83e-118 | 345 |
| MS.gene66499.t1 | MTR_3g008500 | 52.888 | 329 | 129 | 3 | 65 | 370 | 62 | 387 | 5.12e-118 | 347 |
| MS.gene66499.t1 | MTR_4g036090 | 55.161 | 310 | 117 | 2 | 68 | 355 | 59 | 368 | 1.23e-112 | 336 |
| MS.gene66499.t1 | MTR_3g091010 | 50.871 | 287 | 130 | 1 | 93 | 368 | 69 | 355 | 1.69e-101 | 305 |
| MS.gene66499.t1 | MTR_8g027765 | 48.432 | 287 | 135 | 3 | 93 | 367 | 25 | 310 | 1.61e-96 | 290 |
| MS.gene66499.t1 | MTR_4g036685 | 42.231 | 251 | 103 | 7 | 85 | 310 | 22 | 255 | 1.27e-46 | 160 |
| MS.gene66499.t1 | MTR_4g128870 | 68.182 | 110 | 14 | 3 | 108 | 217 | 1 | 89 | 5.02e-40 | 137 |
| MS.gene66499.t1 | MTR_8g090155 | 89.796 | 49 | 5 | 0 | 48 | 96 | 13 | 61 | 9.95e-25 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66499.t1 | AT1G78090 | 59.893 | 374 | 129 | 5 | 8 | 369 | 6 | 370 | 3.21e-153 | 437 |
| MS.gene66499.t1 | AT5G65140 | 61.538 | 351 | 129 | 3 | 20 | 367 | 20 | 367 | 5.83e-152 | 433 |
| MS.gene66499.t1 | AT4G39770 | 63.551 | 321 | 112 | 2 | 52 | 370 | 30 | 347 | 3.62e-150 | 428 |
| MS.gene66499.t1 | AT5G10100 | 61.976 | 334 | 121 | 2 | 42 | 371 | 37 | 368 | 7.35e-150 | 428 |
| MS.gene66499.t1 | AT5G10100 | 61.976 | 334 | 121 | 2 | 42 | 371 | 48 | 379 | 7.59e-150 | 428 |
| MS.gene66499.t1 | AT2G22190 | 60.870 | 345 | 131 | 2 | 24 | 368 | 10 | 350 | 2.88e-149 | 426 |
| MS.gene66499.t1 | AT1G35910 | 63.063 | 333 | 111 | 4 | 47 | 371 | 39 | 367 | 4.23e-148 | 423 |
| MS.gene66499.t1 | AT1G78090 | 60.278 | 360 | 122 | 5 | 8 | 355 | 6 | 356 | 9.28e-147 | 419 |
| MS.gene66499.t1 | AT1G22210 | 63.462 | 312 | 104 | 3 | 65 | 369 | 9 | 317 | 1.50e-144 | 412 |
| MS.gene66499.t1 | AT4G39770 | 64.379 | 306 | 104 | 2 | 52 | 355 | 30 | 332 | 3.22e-144 | 412 |
| MS.gene66499.t1 | AT2G22190 | 61.446 | 332 | 124 | 2 | 24 | 355 | 10 | 337 | 3.73e-144 | 413 |
| MS.gene66499.t1 | AT5G10100 | 62.264 | 318 | 114 | 2 | 42 | 355 | 37 | 352 | 5.23e-143 | 410 |
| MS.gene66499.t1 | AT5G10100 | 57.186 | 334 | 117 | 3 | 42 | 371 | 48 | 359 | 6.68e-131 | 379 |
| MS.gene66499.t1 | AT5G65140 | 58.104 | 327 | 130 | 4 | 2 | 324 | 1 | 324 | 6.93e-129 | 373 |
| MS.gene66499.t1 | AT5G51460 | 50.685 | 365 | 163 | 4 | 21 | 368 | 18 | 382 | 1.97e-120 | 353 |
| MS.gene66499.t1 | AT5G51460 | 50.685 | 365 | 163 | 4 | 21 | 368 | 18 | 382 | 1.97e-120 | 353 |
| MS.gene66499.t1 | AT4G22590 | 53.870 | 323 | 130 | 3 | 67 | 370 | 50 | 372 | 1.80e-119 | 351 |
| MS.gene66499.t1 | AT5G51460 | 50.546 | 366 | 163 | 4 | 21 | 368 | 18 | 383 | 9.11e-119 | 349 |
| MS.gene66499.t1 | AT5G51460 | 50.546 | 366 | 163 | 4 | 21 | 368 | 18 | 383 | 9.11e-119 | 349 |
| MS.gene66499.t1 | AT5G51460 | 50.546 | 366 | 163 | 4 | 21 | 368 | 18 | 383 | 9.11e-119 | 349 |
| MS.gene66499.t1 | AT4G12430 | 53.727 | 322 | 136 | 2 | 64 | 372 | 44 | 365 | 2.39e-116 | 343 |
| MS.gene66499.t1 | AT5G65140 | 59.155 | 284 | 110 | 3 | 20 | 300 | 20 | 300 | 1.05e-112 | 332 |
Find 79 sgRNAs with CRISPR-Local
Find 150 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCCCTTGTCCCATTTAA+TGG | 0.107672 | 5.3:+49724023 | None:intergenic |
| AAATTCGACCAACCATTAAA+TGG | 0.184228 | 5.3:-49724035 | MS.gene66499:CDS |
| AACTTCAGTGTCTGAATTAA+TGG | 0.186430 | 5.3:-49725559 | MS.gene66499:CDS |
| TTACTCATGTAGGCTTTATC+TGG | 0.261967 | 5.3:+49725056 | None:intergenic |
| AAGCCATGGGATGGATATTA+AGG | 0.269464 | 5.3:-49724720 | MS.gene66499:CDS |
| CCTTGTGTTAGCTTTAGTTT+TGG | 0.279314 | 5.3:+49724072 | None:intergenic |
| AATTCAGACACTGAAGTTCT+TGG | 0.288315 | 5.3:+49725564 | None:intergenic |
| TTAGTGCTTCTCCTATTTGT+TGG | 0.289846 | 5.3:+49724695 | None:intergenic |
| GAGAAAACGAAGTGTGTTCC+AGG | 0.327309 | 5.3:-49724388 | MS.gene66499:CDS |
| CGCAATAGGGGCCAAGGGTG+TGG | 0.335378 | 5.3:-49723484 | MS.gene66499:CDS |
| TAAAGCCTACATGAGTAAAA+AGG | 0.339761 | 5.3:-49725051 | MS.gene66499:intron |
| GGCTTACAGTCCAATGAGTT+TGG | 0.362955 | 5.3:+49725379 | None:intergenic |
| AAACAATCTCATTAAGCCTT+TGG | 0.363708 | 5.3:-49725505 | MS.gene66499:CDS |
| TTATCTGGATCTGCTACAAT+TGG | 0.368355 | 5.3:+49725071 | None:intergenic |
| CAAATAATGTACAATACAAA+TGG | 0.375691 | 5.3:-49725137 | MS.gene66499:CDS |
| AATGTATTTCCAATCTATAT+TGG | 0.376405 | 5.3:-49723686 | MS.gene66499:CDS |
| TGTAGCTATTTAGCTTTGTA+AGG | 0.388676 | 5.3:-49724766 | MS.gene66499:intron |
| GCTATTTAGCTTTGTAAGGT+TGG | 0.390975 | 5.3:-49724762 | MS.gene66499:CDS |
| GATTGTGAATGAGAGGTTAA+TGG | 0.392429 | 5.3:-49725622 | MS.gene66499:CDS |
| TTTCCCACAGCCATTGTTAG+TGG | 0.400255 | 5.3:-49724902 | MS.gene66499:CDS |
| GAATTAATGGCTTTGTTTGG+TGG | 0.404929 | 5.3:-49725546 | MS.gene66499:CDS |
| TCTGAATTAATGGCTTTGTT+TGG | 0.409233 | 5.3:-49725549 | MS.gene66499:CDS |
| CCCTTGTCCCATTTAATGGT+TGG | 0.411248 | 5.3:+49724027 | None:intergenic |
| AATTAATGGCTTTGTTTGGT+GGG | 0.420794 | 5.3:-49725545 | MS.gene66499:CDS |
| TATTTAGAGTTGGGCACCAT+TGG | 0.421897 | 5.3:-49724127 | MS.gene66499:intron |
| GGGTCAGGTTTATAAGATCT+TGG | 0.424468 | 5.3:-49724413 | MS.gene66499:intron |
| TATCTGGATCTGCTACAATT+GGG | 0.442252 | 5.3:+49725072 | None:intergenic |
| GCAATAGGGGCCAAGGGTGT+GGG | 0.454198 | 5.3:-49723483 | MS.gene66499:CDS |
| ATATTAAGGGTCCAACAAAT+AGG | 0.455858 | 5.3:-49724706 | MS.gene66499:CDS |
| CGATCGTCACCAATATAGAT+TGG | 0.456578 | 5.3:+49723677 | None:intergenic |
| AACCGATGAAGATGCTTTCA+AGG | 0.459255 | 5.3:-49723654 | MS.gene66499:intron |
| CTTGAATTTCTTACCTCAGG+TGG | 0.462681 | 5.3:+49723406 | None:intergenic |
| TCTTGGTAAAACATTAGAGT+TGG | 0.472981 | 5.3:+49725581 | None:intergenic |
| AAAATCGTGACCAAACTCAT+TGG | 0.474132 | 5.3:-49725389 | MS.gene66499:CDS |
| GCTACGAACCTCATTGATCA+TGG | 0.476130 | 5.3:+49724538 | None:intergenic |
| ATGACTAACCAGAATATGTT+GGG | 0.482279 | 5.3:-49725657 | None:intergenic |
| AATTCGACCAACCATTAAAT+GGG | 0.483720 | 5.3:-49724034 | MS.gene66499:CDS |
| TTATACTATGCTGGAAGCCA+TGG | 0.484718 | 5.3:-49724734 | MS.gene66499:CDS |
| CCATTAAATGGGACAAGGGA+AGG | 0.493371 | 5.3:-49724023 | MS.gene66499:CDS |
| AATGACTAACCAGAATATGT+TGG | 0.502586 | 5.3:-49725658 | None:intergenic |
| TTGGCTGAATTATACTATGC+TGG | 0.505769 | 5.3:-49724743 | MS.gene66499:CDS |
| AAATAATGTACAATACAAAT+GGG | 0.510120 | 5.3:-49725136 | MS.gene66499:CDS |
| TTAGATGAGAGTGACATTGA+AGG | 0.514001 | 5.3:-49724939 | MS.gene66499:intron |
| GAAGTGTGTTCCAGGGGCTA+TGG | 0.519970 | 5.3:-49724380 | MS.gene66499:CDS |
| AGCCATGGGATGGATATTAA+GGG | 0.535930 | 5.3:-49724719 | MS.gene66499:CDS |
| TACCTTGAAAGCATCTTCAT+CGG | 0.538497 | 5.3:+49723652 | None:intergenic |
| AACACAAGGGAGAAAAGTGT+TGG | 0.542726 | 5.3:-49724058 | MS.gene66499:CDS |
| AGTAGGAGAAGAAGCTCTCA+TGG | 0.543615 | 5.3:+49725431 | None:intergenic |
| TTGAACATGTTCAAAGCAGA+AGG | 0.546219 | 5.3:+49725161 | None:intergenic |
| GACCCTTAATATCCATCCCA+TGG | 0.547681 | 5.3:+49724717 | None:intergenic |
| GTGTGTTCCAGGGGCTATGG+TGG | 0.549508 | 5.3:-49724377 | MS.gene66499:CDS |
| TCTTCCACTAACAATGGCTG+TGG | 0.554269 | 5.3:+49724898 | None:intergenic |
| ATACAAGAATCCCACACCCT+TGG | 0.561032 | 5.3:+49723473 | None:intergenic |
| ATCTGGATCTGCTACAATTG+GGG | 0.562431 | 5.3:+49725073 | None:intergenic |
| AAGCTTACTTGTTCAGCCAA+TGG | 0.567677 | 5.3:+49724111 | None:intergenic |
| CATTAAATGGGACAAGGGAA+GGG | 0.571676 | 5.3:-49724022 | MS.gene66499:CDS |
| ACATGTTCAAAGCAGAAGGA+TGG | 0.573560 | 5.3:+49725165 | None:intergenic |
| TGATCATTATCATCTTCCAA+AGG | 0.579315 | 5.3:+49725489 | None:intergenic |
| ATGGGCAAGAATTCACTAGC+AGG | 0.580507 | 5.3:+49724557 | None:intergenic |
| GATTCGTGTCTGGTTCGAGT+AGG | 0.581250 | 5.3:+49725414 | None:intergenic |
| CTATGCTGGAAGCCATGGGA+TGG | 0.581523 | 5.3:-49724729 | MS.gene66499:CDS |
| CCAAAACTAAAGCTAACACA+AGG | 0.588195 | 5.3:-49724072 | MS.gene66499:CDS |
| CTACGAACCTCATTGATCAT+GGG | 0.600560 | 5.3:+49724539 | None:intergenic |
| TCAGGTTTATAAGATCTTGG+TGG | 0.601263 | 5.3:-49724410 | MS.gene66499:intron |
| TATACTATGCTGGAAGCCAT+GGG | 0.609558 | 5.3:-49724733 | MS.gene66499:CDS |
| ACCAACCATTAAATGGGACA+AGG | 0.627155 | 5.3:-49724028 | MS.gene66499:CDS |
| TAAGCATCTTCCACTAACAA+TGG | 0.635138 | 5.3:+49724892 | None:intergenic |
| AGAAAACGAAGTGTGTTCCA+GGG | 0.637437 | 5.3:-49724387 | MS.gene66499:CDS |
| ATTTCAGGGTAATAATGATG+AGG | 0.638286 | 5.3:-49724591 | MS.gene66499:intron |
| CTTCCACTAACAATGGCTGT+GGG | 0.639958 | 5.3:+49724899 | None:intergenic |
| ATTCTTGCCCATGATCAATG+AGG | 0.645083 | 5.3:-49724546 | MS.gene66499:intron |
| GAAGATGATAATGATCACAA+AGG | 0.653543 | 5.3:-49725483 | MS.gene66499:CDS |
| CACGTGTGATTGTGAATGAG+AGG | 0.660978 | 5.3:-49725629 | MS.gene66499:CDS |
| GAGCAACTAAAGTTAACGCG+TGG | 0.664842 | 5.3:-49725461 | MS.gene66499:CDS |
| GAAAACGAAGTGTGTTCCAG+GGG | 0.667596 | 5.3:-49724386 | MS.gene66499:CDS |
| CACTTTGCAAGATCCACCTG+AGG | 0.668399 | 5.3:-49723419 | MS.gene66499:intron |
| CCAACCATTAAATGGGACAA+GGG | 0.680428 | 5.3:-49724027 | MS.gene66499:CDS |
| CAAAACTAAAGCTAACACAA+GGG | 0.690741 | 5.3:-49724071 | MS.gene66499:CDS |
| TAGTGGAAGATGCTTAGACA+AGG | 0.695626 | 5.3:-49724885 | MS.gene66499:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAAATAAAATAAATT+AGG | + | chr5.3:49724468-49724487 | None:intergenic | 0.0% |
| !!! | TTTATTTTATTTTTATATTT+GGG | - | chr5.3:49724475-49724494 | MS.gene66499:intron | 0.0% |
| !!! | TTTTATTTTATTTTTATATT+TGG | - | chr5.3:49724474-49724493 | MS.gene66499:intron | 0.0% |
| !! | AATTAATTCCATTAATAAAT+TGG | - | chr5.3:49724975-49724994 | MS.gene66499:intron | 10.0% |
| !!! | TAATTTTTATAAAGTTAGTA+AGG | + | chr5.3:49725293-49725312 | None:intergenic | 10.0% |
| !!! | TTCATTTAAATTTTGTATAT+AGG | - | chr5.3:49725580-49725599 | MS.gene66499:CDS | 10.0% |
| !! | AAATAATGTACAATACAAAT+GGG | - | chr5.3:49723772-49723791 | MS.gene66499:intron | 15.0% |
| !! | AATAAAAATGTTAGTATATG+TGG | + | chr5.3:49725331-49725350 | None:intergenic | 15.0% |
| !! | AATTTGGATATGAATAATAT+AGG | - | chr5.3:49725396-49725415 | MS.gene66499:CDS | 15.0% |
| !! | ATTTCATCTATTATTATTTC+AGG | - | chr5.3:49724302-49724321 | MS.gene66499:intron | 15.0% |
| !! | TATTTGTACCAATTTATTAA+TGG | + | chr5.3:49724986-49725005 | None:intergenic | 15.0% |
| !! | TTTCATCTATTATTATTTCA+GGG | - | chr5.3:49724303-49724322 | MS.gene66499:intron | 15.0% |
| !!! | ATTTTGTTTTATTTAGAGTT+GGG | - | chr5.3:49724772-49724791 | MS.gene66499:intron | 15.0% |
| !!! | TTTAAAGTATTTCTATACTT+AGG | - | chr5.3:49724641-49724660 | MS.gene66499:intron | 15.0% |
| !!! | TTTAAATTTTGTATATAGGT+TGG | - | chr5.3:49725584-49725603 | MS.gene66499:CDS | 15.0% |
| !! | AAATAAAATAAATTAGGCAC+AGG | + | chr5.3:49724462-49724481 | None:intergenic | 20.0% |
| !! | AATGTATTTCCAATCTATAT+TGG | - | chr5.3:49725222-49725241 | MS.gene66499:intron | 20.0% |
| !! | ATGGTGTAACTAAAAAAATT+TGG | + | chr5.3:49724417-49724436 | None:intergenic | 20.0% |
| !! | CAAATAATGTACAATACAAA+TGG | - | chr5.3:49723771-49723790 | MS.gene66499:intron | 20.0% |
| !! | GTTATGATTCTAAATTATGT+CGG | - | chr5.3:49723588-49723607 | MS.gene66499:intron | 20.0% |
| !! | TTAATGGAATTAATTCTCAA+AGG | + | chr5.3:49724970-49724989 | None:intergenic | 20.0% |
| !!! | ATAACAAAGTTTCTAAAACA+TGG | - | chr5.3:49724745-49724764 | MS.gene66499:CDS | 20.0% |
| !!! | CATTTTGTTTTATTTAGAGT+TGG | - | chr5.3:49724771-49724790 | MS.gene66499:intron | 20.0% |
| !!! | TAAACAAAAGTTGACTTTTT+AGG | - | chr5.3:49725039-49725058 | MS.gene66499:intron | 20.0% |
| !!! | TAACAAAGTTTCTAAAACAT+GGG | - | chr5.3:49724746-49724765 | MS.gene66499:CDS | 20.0% |
| !!! | TTTATTTTTATATTTGGGTC+AGG | - | chr5.3:49724480-49724499 | MS.gene66499:intron | 20.0% |
| ! | ATAAAACACGTTAATGATGA+TGG | - | chr5.3:49724689-49724708 | MS.gene66499:intron | 25.0% |
| ! | CATTCAATAAATGATGTATG+TGG | + | chr5.3:49725083-49725064 | None:intergenic | 25.0% |
| ! | GCAAGTTAGAATTATGATTA+CGG | - | chr5.3:49723665-49723684 | MS.gene66499:CDS | 25.0% |
| ! | GTGTAACTAAAAAAATTTGG+TGG | + | chr5.3:49724414-49724433 | None:intergenic | 25.0% |
| ! | TAAAACACGTTAATGATGAT+GGG | - | chr5.3:49724690-49724709 | MS.gene66499:intron | 25.0% |
| !! | ATAGTTTTTCTTGACTATGA+TGG | - | chr5.3:49723804-49723823 | MS.gene66499:intron | 25.0% |
| !!! | CTTTTGTTTATAATAGTGAC+CGG | + | chr5.3:49725029-49725048 | None:intergenic | 25.0% |
| !!! | TTTTTGGTGTGTAAGTAATA+AGG | + | chr5.3:49723553-49723572 | None:intergenic | 25.0% |
| AAACAATCTCATTAAGCCTT+TGG | - | chr5.3:49723403-49723422 | MS.gene66499:intron | 30.0% | |
| AAATTCGACCAACCATTAAA+TGG | - | chr5.3:49724873-49724892 | MS.gene66499:intron | 30.0% | |
| AACTTCAGTGTCTGAATTAA+TGG | - | chr5.3:49723349-49723368 | MS.gene66499:intron | 30.0% | |
| AATTCGACCAACCATTAAAT+GGG | - | chr5.3:49724874-49724893 | MS.gene66499:intron | 30.0% | |
| ATATTAAGGGTCCAACAAAT+AGG | - | chr5.3:49724202-49724221 | MS.gene66499:intron | 30.0% | |
| ATTTCAGGGTAATAATGATG+AGG | - | chr5.3:49724317-49724336 | MS.gene66499:intron | 30.0% | |
| CAAAACTAAAGCTAACACAA+GGG | - | chr5.3:49724837-49724856 | MS.gene66499:intron | 30.0% | |
| GTTAGAATTATGATTACGGT+TGG | - | chr5.3:49723669-49723688 | MS.gene66499:CDS | 30.0% | |
| TAAAGCCTACATGAGTAAAA+AGG | - | chr5.3:49723857-49723876 | MS.gene66499:intron | 30.0% | |
| TAATTAACACATATTCCCTC+CGG | + | chr5.3:49725159-49725178 | None:intergenic | 30.0% | |
| TCTTGGTAAAACATTAGAGT+TGG | + | chr5.3:49723330-49723349 | None:intergenic | 30.0% | |
| TGATCATTATCATCTTCCAA+AGG | + | chr5.3:49723422-49723441 | None:intergenic | 30.0% | |
| TGCTATACTACAGATAAATG+TGG | + | chr5.3:49723614-49723633 | None:intergenic | 30.0% | |
| ! | AATATAGGTTTTGCGCAATA+GGG | - | chr5.3:49725411-49725430 | MS.gene66499:CDS | 30.0% |
| ! | ACAAACCTTTTTACTCATGT+AGG | + | chr5.3:49723865-49723884 | None:intergenic | 30.0% |
| ! | CTTTTGCTTATAATAGTGAC+CGG | - | chr5.3:49725137-49725156 | MS.gene66499:CDS | 30.0% |
| ! | GAAGATGATAATGATCACAA+AGG | - | chr5.3:49723425-49723444 | MS.gene66499:CDS | 30.0% |
| ! | TAATATAGGTTTTGCGCAAT+AGG | - | chr5.3:49725410-49725429 | MS.gene66499:CDS | 30.0% |
| ! | TTTTCACTAACGTTTGATTC+TGG | - | chr5.3:49723890-49723909 | MS.gene66499:intron | 30.0% |
| !! | AATTAATGGCTTTGTTTGGT+GGG | - | chr5.3:49723363-49723382 | MS.gene66499:intron | 30.0% |
| !! | TATAGGTTGGACAATTTTTG+CGG | - | chr5.3:49725597-49725616 | MS.gene66499:CDS | 30.0% |
| !! | TCTGAATTAATGGCTTTGTT+TGG | - | chr5.3:49723359-49723378 | MS.gene66499:intron | 30.0% |
| !! | TGTAGCTATTTAGCTTTGTA+AGG | - | chr5.3:49724142-49724161 | MS.gene66499:intron | 30.0% |
| !!! | CTAGTCAAACAGTCTTTTTT+TGG | + | chr5.3:49723569-49723588 | None:intergenic | 30.0% |
| !!! | CTTGTGTTAGCTTTAGTTTT+GGG | + | chr5.3:49724838-49724857 | None:intergenic | 30.0% |
| !!! | TAATGAGATTGTTTTTGTGC+TGG | + | chr5.3:49723397-49723416 | None:intergenic | 30.0% |
| AAAATCGTGACCAAACTCAT+TGG | - | chr5.3:49723519-49723538 | MS.gene66499:intron | 35.0% | |
| AATTCAGACACTGAAGTTCT+TGG | + | chr5.3:49723347-49723366 | None:intergenic | 35.0% | |
| AGGTAAAATCCCATACCTAA+TGG | + | chr5.3:49724442-49724461 | None:intergenic | 35.0% | |
| ATGCTTGAATTTCTTACCTC+AGG | + | chr5.3:49725508-49725527 | None:intergenic | 35.0% | |
| CCAAAACTAAAGCTAACACA+AGG | - | chr5.3:49724836-49724855 | MS.gene66499:intron | 35.0% | |
| CTCTATCCAAATTATTGCGA+CGG | - | chr5.3:49724254-49724273 | MS.gene66499:intron | 35.0% | |
| GATTGTGAATGAGAGGTTAA+TGG | - | chr5.3:49723286-49723305 | MS.gene66499:CDS | 35.0% | |
| GGTAAAATCCCATACCTAAT+GGG | + | chr5.3:49724441-49724460 | None:intergenic | 35.0% | |
| TAAGCATCTTCCACTAACAA+TGG | + | chr5.3:49724019-49724038 | None:intergenic | 35.0% | |
| TAATCTCCGTCGCAATAATT+TGG | + | chr5.3:49724263-49724282 | None:intergenic | 35.0% | |
| TATCTGGATCTGCTACAATT+GGG | + | chr5.3:49723839-49723858 | None:intergenic | 35.0% | |
| TCAGGTTTATAAGATCTTGG+TGG | - | chr5.3:49724498-49724517 | MS.gene66499:intron | 35.0% | |
| TGTTTATAATAGTGACCGGA+GGG | + | chr5.3:49725025-49725044 | None:intergenic | 35.0% | |
| TTAGATGAGAGTGACATTGA+AGG | - | chr5.3:49723969-49723988 | MS.gene66499:intron | 35.0% | |
| TTAGTGCTTCTCCTATTTGT+TGG | + | chr5.3:49724216-49724235 | None:intergenic | 35.0% | |
| TTAGTTACACCATTCCCATT+AGG | - | chr5.3:49724424-49724443 | MS.gene66499:intron | 35.0% | |
| TTATCTGGATCTGCTACAAT+TGG | + | chr5.3:49723840-49723859 | None:intergenic | 35.0% | |
| TTGAACATGTTCAAAGCAGA+AGG | + | chr5.3:49723750-49723769 | None:intergenic | 35.0% | |
| TTGGCTGAATTATACTATGC+TGG | - | chr5.3:49724165-49724184 | MS.gene66499:intron | 35.0% | |
| TTGTTTATAATAGTGACCGG+AGG | + | chr5.3:49725026-49725045 | None:intergenic | 35.0% | |
| ! | ATATAGGTTTTGCGCAATAG+GGG | - | chr5.3:49725412-49725431 | MS.gene66499:CDS | 35.0% |
| ! | GAATTTTTGCTAGAGTCACT+TGG | - | chr5.3:49724914-49724933 | MS.gene66499:CDS | 35.0% |
| ! | GCTATGCTAACTTGTCAATT+TGG | - | chr5.3:49725380-49725399 | MS.gene66499:intron | 35.0% |
| ! | TTACTCATGTAGGCTTTATC+TGG | + | chr5.3:49723855-49723874 | None:intergenic | 35.0% |
| !! | GAATTAATGGCTTTGTTTGG+TGG | - | chr5.3:49723362-49723381 | MS.gene66499:intron | 35.0% |
| !! | GCTATTTAGCTTTGTAAGGT+TGG | - | chr5.3:49724146-49724165 | MS.gene66499:intron | 35.0% |
| !! | TACCTTGAAAGCATCTTCAT+CGG | + | chr5.3:49725259-49725278 | None:intergenic | 35.0% |
| !!! | CCTTGTGTTAGCTTTAGTTT+TGG | + | chr5.3:49724839-49724858 | None:intergenic | 35.0% |
| AACCGATGAAGATGCTTTCA+AGG | - | chr5.3:49725254-49725273 | MS.gene66499:intron | 40.0% | |
| AAGCCATGGGATGGATATTA+AGG | - | chr5.3:49724188-49724207 | MS.gene66499:intron | 40.0% | |
| AAGCTTACTTGTTCAGCCAA+TGG | + | chr5.3:49724800-49724819 | None:intergenic | 40.0% | |
| AATCCCATACCTAATGGGAA+TGG | + | chr5.3:49724436-49724455 | None:intergenic | 40.0% | |
| ACACCATTCCCATTAGGTAT+GGG | - | chr5.3:49724430-49724449 | MS.gene66499:intron | 40.0% | |
| ACAGACAAATGTGACTGATG+AGG | - | chr5.3:49723636-49723655 | MS.gene66499:intron | 40.0% | |
| ACATGTTCAAAGCAGAAGGA+TGG | + | chr5.3:49723746-49723765 | None:intergenic | 40.0% | |
| ACCAACCATTAAATGGGACA+AGG | - | chr5.3:49724880-49724899 | MS.gene66499:intron | 40.0% | |
| AGAAAACGAAGTGTGTTCCA+GGG | - | chr5.3:49724521-49724540 | MS.gene66499:intron | 40.0% | |
| AGCCATGGGATGGATATTAA+GGG | - | chr5.3:49724189-49724208 | MS.gene66499:intron | 40.0% | |
| ATCTGGATCTGCTACAATTG+GGG | + | chr5.3:49723838-49723857 | None:intergenic | 40.0% | |
| ATTCTTGCCCATGATCAATG+AGG | - | chr5.3:49724362-49724381 | MS.gene66499:CDS | 40.0% | |
| CATTAAATGGGACAAGGGAA+GGG | - | chr5.3:49724886-49724905 | MS.gene66499:CDS | 40.0% | |
| CCAACCATTAAATGGGACAA+GGG | - | chr5.3:49724881-49724900 | MS.gene66499:intron | 40.0% | |
| CGATCGTCACCAATATAGAT+TGG | + | chr5.3:49725234-49725253 | None:intergenic | 40.0% | |
| CGTTGACGAAAAGGTAACAA+TGG | - | chr5.3:49724591-49724610 | MS.gene66499:intron | 40.0% | |
| CTACGAACCTCATTGATCAT+GGG | + | chr5.3:49724372-49724391 | None:intergenic | 40.0% | |
| CTTGAATTTCTTACCTCAGG+TGG | + | chr5.3:49725505-49725524 | None:intergenic | 40.0% | |
| GGGTCAGGTTTATAAGATCT+TGG | - | chr5.3:49724495-49724514 | MS.gene66499:intron | 40.0% | |
| TACACCATTCCCATTAGGTA+TGG | - | chr5.3:49724429-49724448 | MS.gene66499:intron | 40.0% | |
| TAGTGGAAGATGCTTAGACA+AGG | - | chr5.3:49724023-49724042 | MS.gene66499:CDS | 40.0% | |
| TATACTATGCTGGAAGCCAT+GGG | - | chr5.3:49724175-49724194 | MS.gene66499:intron | 40.0% | |
| TGCTTATAATAGTGACCGGA+GGG | - | chr5.3:49725141-49725160 | MS.gene66499:CDS | 40.0% | |
| TTATACTATGCTGGAAGCCA+TGG | - | chr5.3:49724174-49724193 | MS.gene66499:intron | 40.0% | |
| TTGCTTATAATAGTGACCGG+AGG | - | chr5.3:49725140-49725159 | MS.gene66499:CDS | 40.0% | |
| ! | AACACAAGGGAGAAAAGTGT+TGG | - | chr5.3:49724850-49724869 | MS.gene66499:intron | 40.0% |
| ! | ACAATTTTTGCGGCATTTGG+TGG | - | chr5.3:49725607-49725626 | MS.gene66499:CDS | 40.0% |
| ! | TATTTAGAGTTGGGCACCAT+TGG | - | chr5.3:49724781-49724800 | MS.gene66499:intron | 40.0% |
| ! | TGGACAATTTTTGCGGCATT+TGG | - | chr5.3:49725604-49725623 | MS.gene66499:CDS | 40.0% |
| ! | TTTTCGCAACGTTGACGAAA+AGG | - | chr5.3:49724582-49724601 | MS.gene66499:CDS | 40.0% |
| !! | ACGATTTTCTGATTCGTGTC+TGG | + | chr5.3:49723507-49723526 | None:intergenic | 40.0% |
| AGTAGGAGAAGAAGCTCTCA+TGG | + | chr5.3:49723480-49723499 | None:intergenic | 45.0% | |
| ATACAAGAATCCCACACCCT+TGG | + | chr5.3:49725438-49725457 | None:intergenic | 45.0% | |
| ATGGGCAAGAATTCACTAGC+AGG | + | chr5.3:49724354-49724373 | None:intergenic | 45.0% | |
| CACGTGTGATTGTGAATGAG+AGG | - | chr5.3:49723279-49723298 | MS.gene66499:CDS | 45.0% | |
| CCATTAAATGGGACAAGGGA+AGG | - | chr5.3:49724885-49724904 | MS.gene66499:intron | 45.0% | |
| CCCTTGTCCCATTTAATGGT+TGG | + | chr5.3:49724884-49724903 | None:intergenic | 45.0% | |
| CCTTCCCTTGTCCCATTTAA+TGG | + | chr5.3:49724888-49724907 | None:intergenic | 45.0% | |
| CTTCCACTAACAATGGCTGT+GGG | + | chr5.3:49724012-49724031 | None:intergenic | 45.0% | |
| GAAAACGAAGTGTGTTCCAG+GGG | - | chr5.3:49724522-49724541 | MS.gene66499:intron | 45.0% | |
| GACCCTTAATATCCATCCCA+TGG | + | chr5.3:49724194-49724213 | None:intergenic | 45.0% | |
| GAGAAAACGAAGTGTGTTCC+AGG | - | chr5.3:49724520-49724539 | MS.gene66499:intron | 45.0% | |
| GAGCAACTAAAGTTAACGCG+TGG | - | chr5.3:49723447-49723466 | MS.gene66499:CDS | 45.0% | |
| GCTACGAACCTCATTGATCA+TGG | + | chr5.3:49724373-49724392 | None:intergenic | 45.0% | |
| GGCTTACAGTCCAATGAGTT+TGG | + | chr5.3:49723532-49723551 | None:intergenic | 45.0% | |
| TCTTCCACTAACAATGGCTG+TGG | + | chr5.3:49724013-49724032 | None:intergenic | 45.0% | |
| TTCATGAGATGTACTCCCTC+CGG | - | chr5.3:49725007-49725026 | MS.gene66499:intron | 45.0% | |
| TTTCCCACAGCCATTGTTAG+TGG | - | chr5.3:49724006-49724025 | MS.gene66499:CDS | 45.0% | |
| ! | TTATTTTCCACCATAGCCCC+TGG | + | chr5.3:49724541-49724560 | None:intergenic | 45.0% |
| !! | TTTTGCGGCATTTGGTGGAT+TGG | - | chr5.3:49725612-49725631 | MS.gene66499:CDS | 45.0% |
| !!! | GCTTTGTTTGGTGGGCTTTT+AGG | - | chr5.3:49723371-49723390 | MS.gene66499:intron | 45.0% |
| !!! | TATTTTATTTTTTAATATCT+AGG | - | chr5.3:49725183-49725202 | MS.gene66499:intron | 5.0% |
| !!! | TTTTTTCTATATAATTAATT+TGG | - | chr5.3:49724098-49724117 | MS.gene66499:CDS | 5.0% |
| CACTTTGCAAGATCCACCTG+AGG | - | chr5.3:49725489-49725508 | MS.gene66499:CDS | 50.0% | |
| ! | GATTCGTGTCTGGTTCGAGT+AGG | + | chr5.3:49723497-49723516 | None:intergenic | 50.0% |
| ! | TTTTGCGCAATAGGGGCCAA+GGG | - | chr5.3:49725419-49725438 | MS.gene66499:CDS | 50.0% |
| CTATGCTGGAAGCCATGGGA+TGG | - | chr5.3:49724179-49724198 | MS.gene66499:intron | 55.0% | |
| GAAGTGTGTTCCAGGGGCTA+TGG | - | chr5.3:49724528-49724547 | MS.gene66499:intron | 55.0% | |
| ! | GTTTTGCGCAATAGGGGCCA+AGG | - | chr5.3:49725418-49725437 | MS.gene66499:CDS | 55.0% |
| GTGTGTTCCAGGGGCTATGG+TGG | - | chr5.3:49724531-49724550 | MS.gene66499:intron | 60.0% | |
| ! | GCAATAGGGGCCAAGGGTGT+GGG | - | chr5.3:49725425-49725444 | MS.gene66499:CDS | 60.0% |
| CGCAATAGGGGCCAAGGGTG+TGG | - | chr5.3:49725424-49725443 | MS.gene66499:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 49723263 | 49725667 | 49723263 | ID=MS.gene66499 |
| chr5.3 | mRNA | 49723263 | 49725667 | 49723263 | ID=MS.gene66499.t1;Parent=MS.gene66499 |
| chr5.3 | exon | 49725386 | 49725667 | 49725386 | ID=MS.gene66499.t1.exon1;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49725386 | 49725667 | 49725386 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49725052 | 49725192 | 49725052 | ID=MS.gene66499.t1.exon2;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49725052 | 49725192 | 49725052 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49724886 | 49724957 | 49724886 | ID=MS.gene66499.t1.exon3;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49724886 | 49724957 | 49724886 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49724691 | 49724783 | 49724691 | ID=MS.gene66499.t1.exon4;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49724691 | 49724783 | 49724691 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49724547 | 49724606 | 49724547 | ID=MS.gene66499.t1.exon5;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49724547 | 49724606 | 49724547 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49724327 | 49724428 | 49724327 | ID=MS.gene66499.t1.exon6;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49724327 | 49724428 | 49724327 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49723995 | 49724142 | 49723995 | ID=MS.gene66499.t1.exon7;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49723995 | 49724142 | 49723995 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49723655 | 49723725 | 49723655 | ID=MS.gene66499.t1.exon8;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49723655 | 49723725 | 49723655 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49723420 | 49723512 | 49723420 | ID=MS.gene66499.t1.exon9;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49723420 | 49723512 | 49723420 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| chr5.3 | exon | 49723263 | 49723328 | 49723263 | ID=MS.gene66499.t1.exon10;Parent=MS.gene66499.t1 |
| chr5.3 | CDS | 49723263 | 49723328 | 49723263 | ID=cds.MS.gene66499.t1;Parent=MS.gene66499.t1 |
| Gene Sequence |
| Protein sequence |
