AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66593

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66593.t1 MTR_8g102530 97.253 182 5 0 1 182 1 182 1.70e-132 368
MS.gene66593.t1 MTR_8g102530 92.857 182 4 1 1 182 1 173 6.66e-123 344
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66593.t1 AT5G15750 79.006 181 38 0 1 181 1 181 2.90e-108 307

Find 40 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGGTCTGTAATTGTATT+TGG 0.229997 4.3:+49950943 None:intergenic
TGATATGACTGATAAGCTTT+TGG 0.287922 4.3:-49952658 MS.gene66593:CDS
AGCTGTAACTTATATTGAAC+AGG 0.366283 4.3:-49950983 MS.gene66593:CDS
AAGCACCTTTCTCTTTATCT+TGG 0.381545 4.3:+49950870 None:intergenic
ACGTAGGCTCTCAACTGTTT+TGG 0.388493 4.3:-49951037 MS.gene66593:CDS
TTTAATGACAGTTACAATAT+GGG 0.424343 4.3:-49952068 MS.gene66593:intron
ATCAACTCGGAAAGGTTCCT+TGG 0.425381 4.3:+49952677 None:intergenic
CTTCCATGTTTCTAGTTACA+AGG 0.426456 4.3:+49950917 None:intergenic
TTTCTAGTTACAAGGTAAGC+TGG 0.444914 4.3:+49950925 None:intergenic
ACATAGTGTGATGCTTTGCC+TGG 0.454332 4.3:+49952034 None:intergenic
TTGGAATGGAAGAGAGAAGG+AGG 0.499925 4.3:-49952940 MS.gene66593:CDS
ATGCCGGATGGTTCAGAAGT+TGG 0.502345 4.3:-49952724 MS.gene66593:CDS
ATGCAGCGCTACCATGTTAC+CGG 0.507249 4.3:-49952898 MS.gene66593:CDS
TCAACTCGGAAAGGTTCCTT+GGG 0.524183 4.3:+49952678 None:intergenic
TTCTAGTTACAAGGTAAGCT+GGG 0.525625 4.3:+49950926 None:intergenic
AGTGTGATGCTTTGCCTGGT+TGG 0.526362 4.3:+49952038 None:intergenic
TAACCGTGTCAGCCTTATGC+AGG 0.538554 4.3:-49952004 MS.gene66593:intron
GCGCTACCATGTTACCGGTC+GGG 0.546021 4.3:-49952893 MS.gene66593:CDS
AATCGTCCCGACCGGTAACA+TGG 0.550659 4.3:+49952887 None:intergenic
ACATGGAAGACATGATTACA+TGG 0.553951 4.3:-49950903 MS.gene66593:CDS
TTACCAACTTCTGAACCATC+CGG 0.558041 4.3:+49952721 None:intergenic
TTCTTCCAAGATAAAGAGAA+AGG 0.564085 4.3:-49950875 MS.gene66593:CDS
AGGAGGTCACAGAGAGAACA+TGG 0.568486 4.3:-49952923 MS.gene66593:CDS
ATAGGTATTCGAGTATATGC+CGG 0.597856 4.3:-49952740 MS.gene66593:intron
GAACAGGGACATGTTCGAGT+TGG 0.599994 4.3:-49950967 MS.gene66593:CDS
GGTAAACACGGTGAAACAGA+TGG 0.602021 4.3:-49952703 MS.gene66593:CDS
GCTTCAGTACAATGACAAGT+TGG 0.608210 4.3:-49950851 MS.gene66593:CDS
TTACCTGCATAAGGCTGACA+CGG 0.608409 4.3:+49952001 None:intergenic
GGAAGACATGATTACATGGG+TGG 0.620356 4.3:-49950899 MS.gene66593:CDS
AGCTAAAGCATCATGAGCAT+AGG 0.623806 4.3:-49952984 MS.gene66593:CDS
TCAGTCATATCAACTCGGAA+AGG 0.626960 4.3:+49952669 None:intergenic
AGCGCTACCATGTTACCGGT+CGG 0.636484 4.3:-49952894 MS.gene66593:CDS
GGTTCAGAAGTTGGTAAACA+CGG 0.643580 4.3:-49952715 MS.gene66593:CDS
GGTGAAACAGATGGATCCCA+AGG 0.652966 4.3:-49952694 MS.gene66593:CDS
TTACCTTGTAACTAGAAACA+TGG 0.656200 4.3:-49950920 MS.gene66593:CDS
ATATGGGTGTGATTCCAACC+AGG 0.661105 4.3:-49952052 MS.gene66593:CDS
GCTTATCAGTCATATCAACT+CGG 0.663758 4.3:+49952664 None:intergenic
GTATTCGAGTATATGCCGGA+TGG 0.685170 4.3:-49952736 MS.gene66593:CDS
CATGGAAGACATGATTACAT+GGG 0.691863 4.3:-49950902 MS.gene66593:CDS
GCTGTAACTTATATTGAACA+GGG 0.722848 4.3:-49950982 MS.gene66593:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAAATTTAATAGAAAAAT+AGG - chr4.3:49952470-49952489 MS.gene66593:intron 10.0%
!! TAATATTTGTTTCATAATTA+TGG - chr4.3:49951242-49951261 MS.gene66593:intron 10.0%
!!! ATTTTCATATGAAAAAAATA+AGG - chr4.3:49951908-49951927 MS.gene66593:intron 10.0%
!! TAAATTATATACAAGTTACA+GGG - chr4.3:49951411-49951430 MS.gene66593:intron 15.0%
!! TAAGTTATATAACAAAACTA+TGG + chr4.3:49951548-49951567 None:intergenic 15.0%
!!! TATTTTTCTTTGTTTTAGTT+TGG - chr4.3:49951004-49951023 MS.gene66593:CDS 15.0%
!! AAATGAAATGTGATGAAAAT+AGG - chr4.3:49951480-49951499 MS.gene66593:intron 20.0%
!! AGTTAATAAAGTTGAAACAT+TGG - chr4.3:49952632-49952651 MS.gene66593:intron 20.0%
!! GTAAATTATATACAAGTTAC+AGG - chr4.3:49951410-49951429 MS.gene66593:intron 20.0%
!! TTTAATGACAGTTACAATAT+GGG - chr4.3:49951751-49951770 MS.gene66593:intron 20.0%
!! TTTATGACATTCACATAAAA+TGG + chr4.3:49952451-49952470 None:intergenic 20.0%
!!! TATTTTCATCACATTTCATT+TGG + chr4.3:49951481-49951500 None:intergenic 20.0%
!!! TTGAAGAATTTCATTTTTCA+GGG + chr4.3:49952501-49952520 None:intergenic 20.0%
!!! TTTTAATGACAGTTACAATA+TGG - chr4.3:49951750-49951769 MS.gene66593:intron 20.0%
!!! TTTTTTATATCAAACCCATT+TGG + chr4.3:49952018-49952037 None:intergenic 20.0%
! AGATTATGTCAAAAGAAAGA+TGG + chr4.3:49951304-49951323 None:intergenic 25.0%
! ATGACATAAATAGTGAATAC+AGG - chr4.3:49951452-49951471 MS.gene66593:intron 25.0%
! CTATCTATCGAAATATTTAG+TGG - chr4.3:49952073-49952092 MS.gene66593:intron 25.0%
! TATAACTTACAAGTTTGTCT+AGG - chr4.3:49951559-49951578 MS.gene66593:intron 25.0%
! TGAATGAATTACTGCATAAA+TGG - chr4.3:49952048-49952067 MS.gene66593:CDS 25.0%
!! ATTCATTTTCTTTGTGGAAT+TGG - chr4.3:49951037-49951056 MS.gene66593:CDS 25.0%
!! ATTGTGGTGGAAATATTAAT+AGG - chr4.3:49951061-49951080 MS.gene66593:intron 25.0%
!! TAAAGTTGAAACATTGGTTA+TGG - chr4.3:49952638-49952657 MS.gene66593:intron 25.0%
!! TTCATTTTCTTTGTGGAATT+GGG - chr4.3:49951038-49951057 MS.gene66593:CDS 25.0%
!! TTTTATGTTGTTCTTGCATT+TGG - chr4.3:49950981-49951000 MS.gene66593:CDS 25.0%
!!! AACAATCATCCTTTTTGAAA+TGG + chr4.3:49951612-49951631 None:intergenic 25.0%
!!! GAGGTATTAATGAACATTTT+AGG - chr4.3:49952199-49952218 MS.gene66593:intron 25.0%
!!! GTTGAAGAATTTCATTTTTC+AGG + chr4.3:49952502-49952521 None:intergenic 25.0%
!!! TGAAGAATTTCATTTTTCAG+GGG + chr4.3:49952500-49952519 None:intergenic 25.0%
!!! TTTGAGAAAGGTGAATTTTT+TGG - chr4.3:49950860-49950879 MS.gene66593:CDS 25.0%
AAAACTAACAAGACGAGATT+AGG + chr4.3:49952673-49952692 None:intergenic 30.0%
AAACTAACAAGACGAGATTA+GGG + chr4.3:49952672-49952691 None:intergenic 30.0%
AACAAAATTCGGAGCAAATT+AGG + chr4.3:49952749-49952768 None:intergenic 30.0%
AGCTGTAACTTATATTGAAC+AGG - chr4.3:49952836-49952855 MS.gene66593:intron 30.0%
AGTGTCTATAGTTATGGATT+TGG - chr4.3:49952174-49952193 MS.gene66593:intron 30.0%
ATAAGAAGCAAAAAGAAAGC+AGG + chr4.3:49951646-49951665 None:intergenic 30.0%
ATTATATACAAGTTACAGGG+TGG - chr4.3:49951414-49951433 MS.gene66593:intron 30.0%
CATCAATTCAGTTCAAATGA+AGG + chr4.3:49951338-49951357 None:intergenic 30.0%
GCTGTAACTTATATTGAACA+GGG - chr4.3:49952837-49952856 MS.gene66593:intron 30.0%
GTAATCATCTAACATTAGCA+TGG - chr4.3:49952289-49952308 MS.gene66593:intron 30.0%
GTGTCTATAGTTATGGATTT+GGG - chr4.3:49952175-49952194 MS.gene66593:intron 30.0%
TAATGAGCAAACAGATAAAG+TGG + chr4.3:49951882-49951901 None:intergenic 30.0%
TCAAAAAGGATGATTGTTCT+AGG - chr4.3:49951614-49951633 MS.gene66593:intron 30.0%
TCATCTTAAACAAATAGTCC+TGG - chr4.3:49951988-49952007 MS.gene66593:intron 30.0%
TTACCTTGTAACTAGAAACA+TGG - chr4.3:49952899-49952918 MS.gene66593:CDS 30.0%
TTATCTTTCTTCTAATGCGA+CGG - chr4.3:49952566-49952585 MS.gene66593:intron 30.0%
TTCTTCCAAGATAAAGAGAA+AGG - chr4.3:49952944-49952963 MS.gene66593:CDS 30.0%
TTTATATTCGCTTTGTAGCT+AGG - chr4.3:49952388-49952407 MS.gene66593:intron 30.0%
! GCATGATAGTGAACAAAATT+CGG + chr4.3:49952760-49952779 None:intergenic 30.0%
! GCTTTCTTTTTGCTTCTTAT+TGG - chr4.3:49951646-49951665 MS.gene66593:intron 30.0%
! TGATATGACTGATAAGCTTT+TGG - chr4.3:49951161-49951180 MS.gene66593:intron 30.0%
!! CAAGAAAGTGTCTATAGTTA+TGG - chr4.3:49952168-49952187 MS.gene66593:intron 30.0%
!! TAGCTGATTCATTTTCTTTG+TGG - chr4.3:49951031-49951050 MS.gene66593:CDS 30.0%
!! TTTTTAGCCCAAAACTTAGT+TGG + chr4.3:49952592-49952611 None:intergenic 30.0%
!!! ACTAAGTTTTGGGCTAAAAA+TGG - chr4.3:49952592-49952611 MS.gene66593:intron 30.0%
!!! CAGTTGTTTTAATACAGTAG+AGG + chr4.3:49952267-49952286 None:intergenic 30.0%
!!! GAAAGGTGAATTTTTTGGAA+TGG - chr4.3:49950865-49950884 MS.gene66593:CDS 30.0%
AAGCACCTTTCTCTTTATCT+TGG + chr4.3:49952952-49952971 None:intergenic 35.0%
ACATGGAAGACATGATTACA+TGG - chr4.3:49952916-49952935 MS.gene66593:CDS 35.0%
ATACTCCTATTTAACTAGCC+TGG + chr4.3:49952119-49952138 None:intergenic 35.0%
ATAGGTATTCGAGTATATGC+CGG - chr4.3:49951079-49951098 MS.gene66593:intron 35.0%
ATCTAACATTAGCATGGACA+TGG - chr4.3:49952295-49952314 MS.gene66593:intron 35.0%
CATGGAAGACATGATTACAT+GGG - chr4.3:49952917-49952936 MS.gene66593:CDS 35.0%
GCTTATCAGTCATATCAACT+CGG + chr4.3:49951158-49951177 None:intergenic 35.0%
TATAGTTATGGATTTGGGTG+AGG - chr4.3:49952180-49952199 MS.gene66593:intron 35.0%
! AGATACGACACTATGAGTTT+TGG - chr4.3:49951359-49951378 MS.gene66593:intron 35.0%
! ATTTTAGGCCTTCAAAGTCT+AGG - chr4.3:49952214-49952233 MS.gene66593:intron 35.0%
! GCCAAGTTATCTAGTTTCAA+GGG - chr4.3:49952334-49952353 MS.gene66593:intron 35.0%
! GGCTAGTTAAATAGGAGTAT+AGG - chr4.3:49952119-49952138 MS.gene66593:intron 35.0%
! TGCCAAGTTATCTAGTTTCA+AGG - chr4.3:49952333-49952352 MS.gene66593:intron 35.0%
! TTAGACCTGCCATTTCAAAA+AGG - chr4.3:49951600-49951619 MS.gene66593:intron 35.0%
! TTCTAGTTACAAGGTAAGCT+GGG + chr4.3:49952896-49952915 None:intergenic 35.0%
!! CTTCCATGTTTCTAGTTACA+AGG + chr4.3:49952905-49952924 None:intergenic 35.0%
!! TTTCTAGTTACAAGGTAAGC+TGG + chr4.3:49952897-49952916 None:intergenic 35.0%
!!! ATCATCCTTTTTGAAATGGC+AGG + chr4.3:49951608-49951627 None:intergenic 35.0%
!!! TTTTTGGAATGGAAGAGAGA+AGG - chr4.3:49950876-49950895 MS.gene66593:CDS 35.0%
AACTGACTGCAATGGTTCAT+TGG - chr4.3:49951505-49951524 MS.gene66593:intron 40.0%
AATAGGTGAACTGACTGCAA+TGG - chr4.3:49951497-49951516 MS.gene66593:intron 40.0%
AATAGTCCTGGAGTTCCAAA+TGG - chr4.3:49952000-49952019 MS.gene66593:intron 40.0%
AGACTGTTCCTAGACTTTGA+AGG + chr4.3:49952225-49952244 None:intergenic 40.0%
ATAGTCCTGGAGTTCCAAAT+GGG - chr4.3:49952001-49952020 MS.gene66593:intron 40.0%
GACGATGTAATGCAAAGTTG+AGG + chr4.3:49952727-49952746 None:intergenic 40.0%
GCCCTTGAAACTAGATAACT+TGG + chr4.3:49952338-49952357 None:intergenic 40.0%
GCTGGGTCTGTAATTGTATT+TGG + chr4.3:49952879-49952898 None:intergenic 40.0%
GCTTCAGTACAATGACAAGT+TGG - chr4.3:49952968-49952987 MS.gene66593:CDS 40.0%
GTCACATGAAGGTCAACATA+TGG - chr4.3:49952365-49952384 MS.gene66593:intron 40.0%
TCACTATTCCGAGTCGTATA+AGG - chr4.3:49952416-49952435 MS.gene66593:intron 40.0%
TCAGTCATATCAACTCGGAA+AGG + chr4.3:49951153-49951172 None:intergenic 40.0%
TCTTTGTGGAATTGGGATTG+TGG - chr4.3:49951045-49951064 MS.gene66593:intron 40.0%
TTACCAACTTCTGAACCATC+CGG + chr4.3:49951101-49951120 None:intergenic 40.0%
TTCACATAAAATGGCACCCT+TGG + chr4.3:49952442-49952461 None:intergenic 40.0%
! AGATTTTGCCAACATGTGGT+TGG - chr4.3:49951940-49951959 MS.gene66593:intron 40.0%
! AGCTAAAGCATCATGAGCAT+AGG - chr4.3:49950835-49950854 MS.gene66593:CDS 40.0%
! CTTCAGATTTTGCCAACATG+TGG - chr4.3:49951936-49951955 MS.gene66593:intron 40.0%
! GGGCAAATTTTGTCACATGA+AGG - chr4.3:49952354-49952373 MS.gene66593:intron 40.0%
! GTGTTCCTTTTACCTGCATA+AGG + chr4.3:49951830-49951849 None:intergenic 40.0%
!! GGTTCAGAAGTTGGTAAACA+CGG - chr4.3:49951104-49951123 MS.gene66593:intron 40.0%
!! TGAGCATAGGCTTTTGAGAA+AGG - chr4.3:49950848-49950867 MS.gene66593:CDS 40.0%
ACATAGTGTGATGCTTTGCC+TGG + chr4.3:49951788-49951807 None:intergenic 45.0%
ATATGGGTGTGATTCCAACC+AGG - chr4.3:49951767-49951786 MS.gene66593:intron 45.0%
ATCAACTCGGAAAGGTTCCT+TGG + chr4.3:49951145-49951164 None:intergenic 45.0%
CAGACATACCAACCACATGT+TGG + chr4.3:49951951-49951970 None:intergenic 45.0%
GGAAGACATGATTACATGGG+TGG - chr4.3:49952920-49952939 MS.gene66593:CDS 45.0%
GGTAAACACGGTGAAACAGA+TGG - chr4.3:49951116-49951135 MS.gene66593:intron 45.0%
GTATTCGAGTATATGCCGGA+TGG - chr4.3:49951083-49951102 MS.gene66593:intron 45.0%
GTTCACTATCATGCAGACGT+AGG - chr4.3:49952766-49952785 MS.gene66593:intron 45.0%
TCAACTCGGAAAGGTTCCTT+GGG + chr4.3:49951144-49951163 None:intergenic 45.0%
TGGTATGTCTGAGTGCTAAG+AGG - chr4.3:49951960-49951979 MS.gene66593:intron 45.0%
TGGTCAAACAAGTGAAGCCA+GGG - chr4.3:49952093-49952112 MS.gene66593:intron 45.0%
TTACCTGCATAAGGCTGACA+CGG + chr4.3:49951821-49951840 None:intergenic 45.0%
TTGGAATGGAAGAGAGAAGG+AGG - chr4.3:49950879-49950898 MS.gene66593:CDS 45.0%
TTGTGGAATTGGGATTGTGG+TGG - chr4.3:49951048-49951067 MS.gene66593:intron 45.0%
! ACGTAGGCTCTCAACTGTTT+TGG - chr4.3:49952782-49952801 MS.gene66593:intron 45.0%
! CTTTTTGTAATCGTCCCGAC+CGG + chr4.3:49950943-49950962 None:intergenic 45.0%
! GTCAGCCTTATGCAGGTAAA+AGG - chr4.3:49951822-49951841 MS.gene66593:intron 45.0%
! TCAAACCCATTTGGAACTCC+AGG + chr4.3:49952009-49952028 None:intergenic 45.0%
AGGAGGTCACAGAGAGAACA+TGG - chr4.3:49950896-49950915 MS.gene66593:CDS 50.0%
AGTGTGATGCTTTGCCTGGT+TGG + chr4.3:49951784-49951803 None:intergenic 50.0%
ATGCAGCGCTACCATGTTAC+CGG - chr4.3:49950921-49950940 MS.gene66593:CDS 50.0%
ATGCCGGATGGTTCAGAAGT+TGG - chr4.3:49951095-49951114 MS.gene66593:intron 50.0%
GAACAGGGACATGTTCGAGT+TGG - chr4.3:49952852-49952871 MS.gene66593:intron 50.0%
GGTGAAACAGATGGATCCCA+AGG - chr4.3:49951125-49951144 MS.gene66593:intron 50.0%
GTGGTCAAACAAGTGAAGCC+AGG - chr4.3:49952092-49952111 MS.gene66593:intron 50.0%
TCCGAGTCGTATAAGGCCAA+GGG - chr4.3:49952423-49952442 MS.gene66593:intron 50.0%
TGTCTGAGTGCTAAGAGGCT+TGG - chr4.3:49951965-49951984 MS.gene66593:intron 50.0%
TTCCGAGTCGTATAAGGCCA+AGG - chr4.3:49952422-49952441 MS.gene66593:intron 50.0%
! CAGGGCCAGGCTAGTTAAAT+AGG - chr4.3:49952111-49952130 MS.gene66593:intron 50.0%
! CTATTTAACTAGCCTGGCCC+TGG + chr4.3:49952113-49952132 None:intergenic 50.0%
! GCGACGGCCAACTAAGTTTT+GGG - chr4.3:49952582-49952601 MS.gene66593:intron 50.0%
! TAACCGTGTCAGCCTTATGC+AGG - chr4.3:49951815-49951834 MS.gene66593:intron 50.0%
! TGCGACGGCCAACTAAGTTT+TGG - chr4.3:49952581-49952600 MS.gene66593:intron 50.0%
!! ACCCTTGGCCTTATACGACT+CGG + chr4.3:49952427-49952446 None:intergenic 50.0%
AAACAAGTGAAGCCAGGGCC+AGG - chr4.3:49952098-49952117 MS.gene66593:intron 55.0%
AATCGTCCCGACCGGTAACA+TGG + chr4.3:49950935-49950954 None:intergenic 55.0%
AGCGCTACCATGTTACCGGT+CGG - chr4.3:49950925-49950944 MS.gene66593:CDS 55.0%
GCGCTACCATGTTACCGGTC+GGG - chr4.3:49950926-49950945 MS.gene66593:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 49950828 49953013 49950828 ID=MS.gene66593
chr4.3 mRNA 49950828 49953013 49950828 ID=MS.gene66593.t1;Parent=MS.gene66593
chr4.3 exon 49952880 49953013 49952880 ID=MS.gene66593.t1.exon1;Parent=MS.gene66593.t1
chr4.3 CDS 49952880 49953013 49952880 ID=cds.MS.gene66593.t1;Parent=MS.gene66593.t1
chr4.3 exon 49952651 49952758 49952651 ID=MS.gene66593.t1.exon2;Parent=MS.gene66593.t1
chr4.3 CDS 49952651 49952758 49952651 ID=cds.MS.gene66593.t1;Parent=MS.gene66593.t1
chr4.3 exon 49952005 49952079 49952005 ID=MS.gene66593.t1.exon3;Parent=MS.gene66593.t1
chr4.3 CDS 49952005 49952079 49952005 ID=cds.MS.gene66593.t1;Parent=MS.gene66593.t1
chr4.3 exon 49950828 49951059 49950828 ID=MS.gene66593.t1.exon4;Parent=MS.gene66593.t1
chr4.3 CDS 49950828 49951059 49950828 ID=cds.MS.gene66593.t1;Parent=MS.gene66593.t1
Gene Sequence

>MS.gene66593

ATGAGGAAGCTAAAGCATCATGAGCATAGGCTTTTGAGAAAGGTGAATTTTTTGGAATGGAAGAGAGAAGGAGGTCACAGAGAGAACATGGTTATGCAGCGCTACCATGTTACCGGTCGGGACGATTACAAAAAGTATTTTCATAATTCACATTTTTATGTTGTTCTTGCATTTGGTATTTTTCTTTGTTTTAGTTTGGTTTTTAGCTGATTCATTTTCTTTGTGGAATTGGGATTGTGGTGGAAATATTAATAGGTATTCGAGTATATGCCGGATGGTTCAGAAGTTGGTAAACACGGTGAAACAGATGGATCCCAAGGAACCTTTCCGAGTTGATATGACTGATAAGCTTTTGGAAAAACTGTAAGTTCACTTTTTGAAGTTCAAAGTTTAAACATATATGATTTCAGTAATTAATATTTGTTTCATAATTATGGTTACTGCTATATATCATAATTATTCATTCACGTTTTCCATCTTTCTTTTGACATAATCTATTTTTTTTTTCCTTCATTTGAACTGAATTGATGTAGATACGACACTATGAGTTTTGGTTAACTTAATTCAATTACAAATATAGTTGTAAATTATATACAAGTTACAGGGTGGTTTTTGAAATCATTCATGACATAAATAGTGAATACAGGTGACCAAATGAAATGTGATGAAAATAGGTGAACTGACTGCAATGGTTCATTGGTTCTCTCGTCATGATGACCATAGTTTTGTTATATAACTTACAAGTTTGTCTAGGATTTTTGAGAATGATAAATTAGACCTGCCATTTCAAAAAGGATGATTGTTCTAGGTTAGTGCCTGCTTTCTTTTTGCTTCTTATTGGTAGCTGATATTAGTTTGAAACAAAGAAGATAAAAGTAAATTTTGTGAGCTATTTGCACGTATCTGTTTCATTCATTCATTTTTTTAATGACAGTTACAATATGGGTGTGATTCCAACCAGGCAAAGCATCACACTATGTGAACGCTTAACCGTGTCAGCCTTATGCAGGTAAAAGGAACACATTGCTGCTTTTAATGAAAATTTAGTTATCCACTTTATCTGTTTGCTCATTAGAATGTATTTTCATATGAAAAAAATAAGGAATAACTTCAGATTTTGCCAACATGTGGTTGGTATGTCTGAGTGCTAAGAGGCTTGGTCATCTTAAACAAATAGTCCTGGAGTTCCAAATGGGTTTGATATAAAAAACTTAACTGATTGAATGAATTACTGCATAAATGGAACTATCTATCGAAATATTTAGTGGTCAAACAAGTGAAGCCAGGGCCAGGCTAGTTAAATAGGAGTATAGGACAAGCAATAAACATAATCTAGAAATCAAGAAAGTGTCTATAGTTATGGATTTGGGTGAGGTATTAATGAACATTTTAGGCCTTCAAAGTCTAGGAACAGTCTAGAAACAGAAGACAGTCTACCTCTACTGTATTAAAACAACTGAAGTAATCATCTAACATTAGCATGGACATGGTGAATGACAATATTTTGCCAAGTTATCTAGTTTCAAGGGCAAATTTTGTCACATGAAGGTCAACATATGGTTTATATTCGCTTTGTAGCTAGGCAAGTTCACTATTCCGAGTCGTATAAGGCCAAGGGTGCCATTTTATGTGAATGTCATAAACTAAATTTAATAGAAAAATAGGCTGACCCCTGAAAAATGAAATTCTTCAACAGTTTTTTTTTCTTTTGCAACATTAGTTGATTATGATTAAGTAATTATCTTTCTTCTAATGCGACGGCCAACTAAGTTTTGGGCTAAAAATGGACTGTTGTCAACTGTCAAGTTAATAAAGTTGAAACATTGGTTATGGATTCTTGACCCTAATCTCGTCTTGTTAGTTTTATGCAGCAATAAAAGTTTATCGATTTTTGTACCTCAACTTTGCATTACATCGTCCTAATTTGCTCCGAATTTTGTTCACTATCATGCAGACGTAGGCTCTCAACTGTTTTGGTGAAACTAAAATTTGCTGAGCATTTGAAAGAAGCTGTAACTTATATTGAACAGGGACATGTTCGAGTTGGTCCAAATACAATTACAGACCCAGCTTACCTTGTAACTAGAAACATGGAAGACATGATTACATGGGTGGATTCTTCCAAGATAAAGAGAAAGGTGCTTCAGTACAATGACAAGTTGGATGACTATGATTTAATGAATTAG
Protein sequence

>MS.gene66593.t1

MRKLKHHEHRLLRKVNFLEWKREGGHRENMVMQRYHVTGRDDYKKYSSICRMVQKLVNTVKQMDPKEPFRVDMTDKLLEKLYNMGVIPTRQSITLCERLTVSALCRRRLSTVLVKLKFAEHLKEAVTYIEQGHVRVGPNTITDPAYLVTRNMEDMITWVDSSKIKRKVLQYNDKLDDYDLMN