Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69442.t1 | XP_013462188.2 | 95.4 | 765 | 30 | 2 | 1 | 760 | 1 | 765 | 0.00E+00 | 1444.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69442.t1 | Q8W4S4 | 70.8 | 765 | 217 | 3 | 1 | 760 | 14 | 777 | 0.0e+00 | 1096.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69442.t1 | A0A072V4D9 | 95.4 | 721 | 28 | 2 | 1 | 716 | 1 | 721 | 0.0e+00 | 1368.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene69442 | 0.827505 | 1.51E-54 | -1.69E-46 |
| MS.gene049786 | MS.gene69442 | 0.847361 | 1.21E-59 | -1.69E-46 |
| MS.gene050085 | MS.gene69442 | 0.80342 | 3.52E-49 | -1.69E-46 |
| MS.gene050114 | MS.gene69442 | 0.815979 | 7.00E-52 | -1.69E-46 |
| MS.gene050288 | MS.gene69442 | 0.806279 | 8.89E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene69442 | 0.832197 | 1.08E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene69442 | 0.814326 | 1.63E-51 | -1.69E-46 |
| MS.gene051184 | MS.gene69442 | 0.801764 | 7.72E-49 | -1.69E-46 |
| MS.gene051245 | MS.gene69442 | 0.815577 | 8.61E-52 | -1.69E-46 |
| MS.gene051452 | MS.gene69442 | 0.82785 | 1.25E-54 | -1.69E-46 |
| MS.gene051756 | MS.gene69442 | 0.81949 | 1.13E-52 | -1.69E-46 |
| MS.gene051757 | MS.gene69442 | 0.834527 | 2.85E-56 | -1.69E-46 |
| MS.gene051831 | MS.gene69442 | 0.804958 | 1.68E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene69442 | 0.828987 | 6.62E-55 | -1.69E-46 |
| MS.gene052434 | MS.gene69442 | 0.840052 | 1.10E-57 | -1.69E-46 |
| MS.gene053381 | MS.gene69442 | 0.842907 | 1.94E-58 | -1.69E-46 |
| MS.gene055603 | MS.gene69442 | 0.817345 | 3.46E-52 | -1.69E-46 |
| MS.gene056181 | MS.gene69442 | 0.806283 | 8.87E-50 | -1.69E-46 |
| MS.gene056787 | MS.gene69442 | 0.844575 | 6.94E-59 | -1.69E-46 |
| MS.gene056986 | MS.gene69442 | 0.843591 | 1.28E-58 | -1.69E-46 |
| MS.gene057094 | MS.gene69442 | 0.800798 | 1.22E-48 | -1.69E-46 |
| MS.gene057140 | MS.gene69442 | 0.816945 | 4.26E-52 | -1.69E-46 |
| MS.gene057234 | MS.gene69442 | 0.816394 | 5.66E-52 | -1.69E-46 |
| MS.gene057999 | MS.gene69442 | 0.824759 | 6.78E-54 | -1.69E-46 |
| MS.gene058055 | MS.gene69442 | 0.804656 | 1.95E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene69442 | 0.804637 | 1.96E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene69442 | 0.801746 | 7.78E-49 | -1.69E-46 |
| MS.gene058646 | MS.gene69442 | 0.80791 | 4.01E-50 | -1.69E-46 |
| MS.gene058654 | MS.gene69442 | 0.826713 | 2.33E-54 | -1.69E-46 |
| MS.gene058906 | MS.gene69442 | 0.807626 | 4.61E-50 | -1.69E-46 |
| MS.gene059050 | MS.gene69442 | 0.835675 | 1.46E-56 | -1.69E-46 |
| MS.gene059360 | MS.gene69442 | 0.810599 | 1.06E-50 | -1.69E-46 |
| MS.gene06010 | MS.gene69442 | 0.820334 | 7.25E-53 | -1.69E-46 |
| MS.gene06011 | MS.gene69442 | 0.851841 | 6.82E-61 | -1.69E-46 |
| MS.gene060869 | MS.gene69442 | 0.805698 | 1.18E-49 | -1.69E-46 |
| MS.gene060870 | MS.gene69442 | 0.812518 | 4.07E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69442.t1 | MTR_3g112500 | 95.423 | 721 | 28 | 2 | 1 | 716 | 1 | 721 | 0.0 | 1397 |
| MS.gene69442.t1 | MTR_4g071070 | 70.812 | 764 | 218 | 2 | 1 | 760 | 14 | 776 | 0.0 | 1129 |
| MS.gene69442.t1 | MTR_2g093210 | 59.085 | 765 | 307 | 3 | 1 | 760 | 11 | 774 | 0.0 | 949 |
| MS.gene69442.t1 | MTR_4g061090 | 59.322 | 767 | 304 | 4 | 1 | 760 | 11 | 776 | 0.0 | 942 |
| MS.gene69442.t1 | MTR_2g093210 | 58.322 | 727 | 297 | 3 | 39 | 760 | 9 | 734 | 0.0 | 889 |
| MS.gene69442.t1 | MTR_2g093210 | 57.784 | 668 | 276 | 3 | 1 | 663 | 11 | 677 | 0.0 | 809 |
| MS.gene69442.t1 | MTR_2g093210 | 53.307 | 499 | 227 | 3 | 1 | 494 | 11 | 508 | 0.0 | 553 |
| MS.gene69442.t1 | MTR_3g112490 | 93.130 | 262 | 0 | 1 | 775 | 1018 | 1 | 262 | 1.40e-173 | 507 |
| MS.gene69442.t1 | MTR_6g033365 | 57.447 | 188 | 77 | 3 | 826 | 1011 | 1 | 187 | 1.98e-69 | 229 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69442.t1 | AT4G39080 | 70.850 | 765 | 217 | 3 | 1 | 760 | 14 | 777 | 0.0 | 1125 |
| MS.gene69442.t1 | AT2G21410 | 70.550 | 764 | 220 | 2 | 1 | 760 | 15 | 777 | 0.0 | 1123 |
| MS.gene69442.t1 | AT2G28520 | 58.984 | 768 | 307 | 3 | 1 | 760 | 11 | 778 | 0.0 | 936 |
| MS.gene69442.t1 | AT1G75510 | 63.492 | 252 | 65 | 4 | 789 | 1014 | 6 | 256 | 3.19e-102 | 321 |
| MS.gene69442.t1 | AT3G52270 | 49.801 | 251 | 96 | 5 | 790 | 1014 | 23 | 269 | 3.35e-76 | 251 |
| MS.gene69442.t1 | AT3G52270 | 43.357 | 143 | 54 | 3 | 790 | 906 | 23 | 164 | 4.21e-30 | 119 |
Find 190 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCTTCCTATCTTCGTTTA+TGG | 0.080046 | 3.3:-92843428 | MS.gene69442:CDS |
| TCCATTCCGTTTGAACTATT+TGG | 0.108443 | 3.3:-92844459 | MS.gene69442:CDS |
| GCGTAAGTTGCATTTATTTA+AGG | 0.166798 | 3.3:-92846750 | MS.gene69442:CDS |
| TTCTATTGATCTAGAAAATT+TGG | 0.182048 | 3.3:-92846675 | MS.gene69442:CDS |
| GTATTCTTGAACAGCCTATT+TGG | 0.197201 | 3.3:-92844013 | MS.gene69442:CDS |
| TTCAGTAAAGCAGATAAAAT+TGG | 0.203541 | 3.3:-92846149 | MS.gene69442:CDS |
| AACAAAATTTGTGATGCTTT+TGG | 0.213249 | 3.3:-92845830 | MS.gene69442:CDS |
| CAAAATAAATACCTTAAAAT+TGG | 0.220511 | 3.3:+92835508 | None:intergenic |
| CACTCCTCATAATTGTAAAA+TGG | 0.223248 | 3.3:-92843982 | MS.gene69442:CDS |
| CGCTGTAATGTTTGGTGATT+GGG | 0.234878 | 3.3:-92844741 | MS.gene69442:CDS |
| GGCTGTTCAAGAATACAATT+TGG | 0.237322 | 3.3:+92844020 | None:intergenic |
| TCGACAGATTTCTTGTATTC+AGG | 0.238269 | 3.3:+92834823 | None:intergenic |
| TATTGACTGTCCATTATTAA+TGG | 0.242494 | 3.3:-92846275 | MS.gene69442:CDS |
| TGCAACTGTTGGTGTCTTAC+TGG | 0.245600 | 3.3:-92842565 | MS.gene69442:intron |
| CATATTTAGAATGTGATAAA+TGG | 0.248964 | 3.3:+92845338 | None:intergenic |
| TCATGGCATATGCTTATTAT+TGG | 0.252181 | 3.3:-92844717 | MS.gene69442:CDS |
| AATGAAGATGTCAATTCTAT+TGG | 0.256189 | 3.3:-92844217 | MS.gene69442:CDS |
| TTCTTACTTGAAAGAACTTT+TGG | 0.258014 | 3.3:+92843867 | None:intergenic |
| CATGAAAACATGGATGATTA+TGG | 0.267501 | 3.3:-92835753 | MS.gene69442:CDS |
| ATTTAAGGAGCAAATGAAAA+AGG | 0.270582 | 3.3:-92846735 | MS.gene69442:CDS |
| TCTAAGTCCAACAGATGATT+TGG | 0.275930 | 3.3:-92843912 | MS.gene69442:CDS |
| AGCAATTTGTTGCAAACAAT+TGG | 0.307815 | 3.3:-92845564 | MS.gene69442:CDS |
| CAGCTCTCCCCGATCTCTTC+TGG | 0.310704 | 3.3:+92835138 | None:intergenic |
| TATTTGAAAGACAGCCTAAC+TGG | 0.312463 | 3.3:-92835096 | MS.gene69442:CDS |
| TTATAACCGGTCTTGTTACT+AGG | 0.312976 | 3.3:-92846123 | MS.gene69442:CDS |
| ATACAATTTGGGGAACAAAT+TGG | 0.314145 | 3.3:+92844032 | None:intergenic |
| CTCCGTGTGCCATGCCATTT+AGG | 0.315514 | 3.3:+92844267 | None:intergenic |
| AGCTTCCTATCTTCGTTTAT+GGG | 0.320291 | 3.3:-92843427 | MS.gene69442:CDS |
| TCAGTAAAGCAGATAAAATT+GGG | 0.320724 | 3.3:-92846148 | MS.gene69442:CDS |
| CAAGAAATTGTAGACGCATA+TGG | 0.321767 | 3.3:-92845023 | MS.gene69442:CDS |
| CATTTGGCGTAGATCCTAAA+TGG | 0.329997 | 3.3:-92844281 | MS.gene69442:CDS |
| TCGCTGTAATGTTTGGTGAT+TGG | 0.333056 | 3.3:-92844742 | MS.gene69442:CDS |
| ATTTGAGCAGTGGAATCTTC+TGG | 0.337268 | 3.3:-92845535 | MS.gene69442:intron |
| ATATGATTCCAAGATTCATT+TGG | 0.339853 | 3.3:+92844187 | None:intergenic |
| ACACACCAGGATTTGTTTCC+TGG | 0.340835 | 3.3:+92844792 | None:intergenic |
| TTTGTATCAGTTTGTTTGAC+TGG | 0.345873 | 3.3:+92835166 | None:intergenic |
| AACACCATTGTTCTCATTGT+TGG | 0.347282 | 3.3:-92842606 | MS.gene69442:CDS |
| GCTGTTCAAGAATACAATTT+GGG | 0.359364 | 3.3:+92844021 | None:intergenic |
| TATACATGTTCCATTAATAA+TGG | 0.365815 | 3.3:+92846265 | None:intergenic |
| CTCTTGAACAAGTTGCTTCA+AGG | 0.372812 | 3.3:+92835072 | None:intergenic |
| AGGATTTGTTTCCTGGTACT+TGG | 0.373003 | 3.3:+92844799 | None:intergenic |
| TATGATTCCAAGATTCATTT+GGG | 0.373807 | 3.3:+92844188 | None:intergenic |
| GTAACCTTCTCTCCCGATAA+AGG | 0.374757 | 3.3:+92846007 | None:intergenic |
| TTCAGGGATGCTACTACAAT+AGG | 0.384365 | 3.3:-92844330 | MS.gene69442:intron |
| TCAAGTAGAGGCAATAGTTC+AGG | 0.392420 | 3.3:-92845108 | MS.gene69442:CDS |
| ATAAAATTGGGGTTTATAAC+CGG | 0.396630 | 3.3:-92846136 | MS.gene69442:CDS |
| TCATGTTCTAGATCCTTTAT+CGG | 0.400532 | 3.3:-92846020 | MS.gene69442:CDS |
| GAAACTAAGCCAACCATTCC+AGG | 0.403360 | 3.3:+92835533 | None:intergenic |
| CTAAGTCCAACAGATGATTT+GGG | 0.404472 | 3.3:-92843911 | MS.gene69442:CDS |
| TCTCTCGTTGACCATGTTGA+TGG | 0.411005 | 3.3:+92846701 | None:intergenic |
| ATCAAATAGGTTAAAAGATG+TGG | 0.412168 | 3.3:-92846782 | MS.gene69442:intron |
| GGTGAAAACCAGCTCTTTGT+TGG | 0.412927 | 3.3:-92843890 | MS.gene69442:CDS |
| ATGAAGATGTCAATTCTATT+GGG | 0.414653 | 3.3:-92844216 | MS.gene69442:CDS |
| TTAGGTTATATTACTATTGT+TGG | 0.418965 | 3.3:-92843764 | MS.gene69442:intron |
| CATGATTTGGCAACAGCAAC+AGG | 0.419759 | 3.3:+92839890 | None:intergenic |
| GGAGTGAAAGATAGCCAAAT+AGG | 0.420903 | 3.3:+92843999 | None:intergenic |
| GAATTCTATTTCGTGCTACT+AGG | 0.420984 | 3.3:-92846078 | MS.gene69442:CDS |
| CAGAAGAGATCGGGGAGAGC+TGG | 0.423650 | 3.3:-92835137 | MS.gene69442:CDS |
| CAGCTCATAAGTTCCTTGAT+TGG | 0.425122 | 3.3:+92834849 | None:intergenic |
| AAGAAATTGTAGACGCATAT+GGG | 0.426376 | 3.3:-92845022 | MS.gene69442:intron |
| ATTCTATTGTATGTATAAGT+TGG | 0.428985 | 3.3:+92843475 | None:intergenic |
| ACTTGGAGACATATTAGAAA+TGG | 0.433033 | 3.3:-92844559 | MS.gene69442:CDS |
| ATTGATCATTCCAATTGAAT+CGG | 0.434902 | 3.3:-92847262 | MS.gene69442:CDS |
| GGAGTATCCCAAATGAATCT+TGG | 0.435333 | 3.3:-92844195 | MS.gene69442:CDS |
| GATCGATGAGCCGATTCAAT+TGG | 0.436497 | 3.3:+92847252 | None:intergenic |
| ATGTTGTTTCTTCAAGAGAA+AGG | 0.437840 | 3.3:+92843705 | None:intergenic |
| TCAATAGACCCTTCAAAAGT+TGG | 0.440735 | 3.3:+92846292 | None:intergenic |
| GTGCGCGACACTTATCCATT+TGG | 0.445671 | 3.3:-92844297 | MS.gene69442:CDS |
| AATTCTATTTCGTGCTACTA+GGG | 0.446400 | 3.3:-92846077 | MS.gene69442:CDS |
| TCAAATAGGTTAAAAGATGT+GGG | 0.447595 | 3.3:-92846781 | MS.gene69442:intron |
| AAAGAGAACCAGAAGAGATC+GGG | 0.447938 | 3.3:-92835146 | MS.gene69442:CDS |
| GTTGAACTTCAAGCTCTCTC+TGG | 0.448824 | 3.3:+92846314 | None:intergenic |
| ATCTCTTACCTTGGCGATCT+AGG | 0.449701 | 3.3:-92847228 | MS.gene69442:CDS |
| CCTACTCTTTGATCTACAAT+GGG | 0.450668 | 3.3:-92843575 | MS.gene69442:CDS |
| TGGATGGTTCTGCCATGATT+TGG | 0.454021 | 3.3:+92839877 | None:intergenic |
| AACTTCAAGCTCTCTCTGGC+GGG | 0.460694 | 3.3:+92846318 | None:intergenic |
| TTCTTCAAGAGAAAGGGCTT+TGG | 0.461252 | 3.3:+92843712 | None:intergenic |
| GAGGGCCCATAAACGAAGAT+AGG | 0.461762 | 3.3:+92843422 | None:intergenic |
| TTAAAGACAACCATTGATGT+AGG | 0.463823 | 3.3:-92845600 | MS.gene69442:CDS |
| AGTTGCTTCAAGGCCCAGTT+AGG | 0.465380 | 3.3:+92835082 | None:intergenic |
| TGTTGTTAATTGCAGAAACT+TGG | 0.465763 | 3.3:-92844576 | MS.gene69442:intron |
| ATCTTCGTTTATGGGCCCTC+AGG | 0.469823 | 3.3:-92843419 | MS.gene69442:intron |
| GGATGCTACTACAATAGGTT+TGG | 0.469912 | 3.3:-92844325 | MS.gene69442:CDS |
| TTGAAAACAACATAAACATA+TGG | 0.470037 | 3.3:-92844140 | MS.gene69442:intron |
| TAGATTTCTTTGTCCTCTCC+CGG | 0.471186 | 3.3:+92835721 | None:intergenic |
| CATGTTCTAGATCCTTTATC+GGG | 0.473691 | 3.3:-92846019 | MS.gene69442:CDS |
| ATGTTTGGTGATTGGGGTCA+TGG | 0.475527 | 3.3:-92844734 | MS.gene69442:CDS |
| CATTTAGGATCTACGCCAAA+TGG | 0.477514 | 3.3:+92844282 | None:intergenic |
| CGCTGAAATCAAACTCCTCA+TGG | 0.478028 | 3.3:+92843511 | None:intergenic |
| TTGGAAAGAGGTTGAGAAGG+TGG | 0.479012 | 3.3:+92839857 | None:intergenic |
| CACCAAACATTACAGCGAAA+AGG | 0.483279 | 3.3:+92844747 | None:intergenic |
| TGTTGTTTCTTCAAGAGAAA+GGG | 0.483549 | 3.3:+92843706 | None:intergenic |
| CATCGATCCATCTCTTACCT+TGG | 0.485763 | 3.3:-92847237 | MS.gene69442:CDS |
| AATAAAAGAGGCGCCAATCA+AGG | 0.487240 | 3.3:-92834862 | MS.gene69442:CDS |
| AAGCTCTCTCTGGCGGGTTG+CGG | 0.488042 | 3.3:+92846324 | None:intergenic |
| CTATTTGGACAATCTGCCTA+TGG | 0.489161 | 3.3:-92844444 | MS.gene69442:CDS |
| GAAGCATAGGTCTGTTGGAA+TGG | 0.489379 | 3.3:+92847011 | None:intergenic |
| ACGCACATGAGGCCAATGCC+TGG | 0.492472 | 3.3:-92835551 | MS.gene69442:CDS |
| TCCAAATAGTTCAAACGGAA+TGG | 0.495363 | 3.3:+92844458 | None:intergenic |
| AATGTCTCCTTGCAGAAGGC+TGG | 0.495741 | 3.3:-92845293 | MS.gene69442:CDS |
| TGAATTAATCTCAATTAGCT+CGG | 0.496624 | 3.3:+92846542 | None:intergenic |
| GATTGTCCAAATAGTTCAAA+CGG | 0.500166 | 3.3:+92844453 | None:intergenic |
| CAGTTTACAATGGAGATGTC+TGG | 0.505356 | 3.3:-92837678 | MS.gene69442:CDS |
| AAGTAGCGGTGGTTCAAACT+TGG | 0.506657 | 3.3:-92839954 | MS.gene69442:CDS |
| CCTGTGAAGCATAGGTCTGT+TGG | 0.509382 | 3.3:+92847006 | None:intergenic |
| GAGATATATTATTATGATGA+TGG | 0.510661 | 3.3:-92844526 | MS.gene69442:CDS |
| GGTGACAAGGTTGCAATGGA+AGG | 0.512352 | 3.3:-92835810 | MS.gene69442:CDS |
| GAACTTCAAGCTCTCTCTGG+CGG | 0.513046 | 3.3:+92846317 | None:intergenic |
| TATTGATCTAGAAAATTTGG+AGG | 0.515471 | 3.3:-92846672 | MS.gene69442:intron |
| TATAACCGGTCTTGTTACTA+GGG | 0.516391 | 3.3:-92846122 | MS.gene69442:CDS |
| CAAAGAGAACCAGAAGAGAT+CGG | 0.521004 | 3.3:-92835147 | MS.gene69442:CDS |
| TGTAGGATCGCGACATCCAT+AGG | 0.523119 | 3.3:+92844428 | None:intergenic |
| TCCTACTCTTTGATCTACAA+TGG | 0.526141 | 3.3:-92843576 | MS.gene69442:CDS |
| ATGGAAAATTGTGCCGGGAG+AGG | 0.527049 | 3.3:-92835734 | MS.gene69442:CDS |
| ATGGAAGATGAAAACAGTTA+CGG | 0.527896 | 3.3:-92839980 | MS.gene69442:CDS |
| CTGTTGCTGTTGCCAAATCA+TGG | 0.528694 | 3.3:-92839889 | MS.gene69442:CDS |
| GACATCGAAGGCAGAGAGAT+CGG | 0.529923 | 3.3:-92839930 | MS.gene69442:CDS |
| CCAACAGACCTATGCTTCAC+AGG | 0.533446 | 3.3:-92847006 | MS.gene69442:intron |
| AAGCCAACCATTCCAGGCAT+TGG | 0.534227 | 3.3:+92835539 | None:intergenic |
| TTTACTCAAGTCATCTGAGA+AGG | 0.535285 | 3.3:+92845793 | None:intergenic |
| TTTAGGTGACAAGGTTGCAA+TGG | 0.535468 | 3.3:-92835814 | MS.gene69442:intron |
| ATAATTGTAAAATGGTGCAC+TGG | 0.535769 | 3.3:-92843974 | MS.gene69442:CDS |
| CGAAATAGAATTCTTTCGAA+AGG | 0.537677 | 3.3:+92846088 | None:intergenic |
| CATACAATAGAATTTGTGCT+TGG | 0.540924 | 3.3:-92843465 | MS.gene69442:CDS |
| AAGCATAGGTCTGTTGGAAT+GGG | 0.545226 | 3.3:+92847012 | None:intergenic |
| AGATCCTTTATCGGGAGAGA+AGG | 0.547646 | 3.3:-92846011 | MS.gene69442:intron |
| TGACAGAATTGCCAAGTACC+AGG | 0.550640 | 3.3:-92844810 | MS.gene69442:intron |
| TTGTGTGTATACAATAAAAG+AGG | 0.555378 | 3.3:-92834874 | MS.gene69442:CDS |
| AGATCGATCAAGAATCAAGA+TGG | 0.555997 | 3.3:-92839999 | MS.gene69442:CDS |
| AAAGAGGTTGAGAAGGTGGA+TGG | 0.557605 | 3.3:+92839861 | None:intergenic |
| ATGATGCAAACCTACATCAA+TGG | 0.557963 | 3.3:+92845590 | None:intergenic |
| TTGGCCTCATGTGCGTTCCA+CGG | 0.560162 | 3.3:+92835558 | None:intergenic |
| ATAATTATGTCAGTTTACAA+TGG | 0.563019 | 3.3:-92837688 | MS.gene69442:intron |
| TTGGATATCAATTTGAGCAG+TGG | 0.566788 | 3.3:-92845545 | MS.gene69442:CDS |
| TGGCACTCATTTCAGTACCA+TGG | 0.568747 | 3.3:-92843744 | MS.gene69442:CDS |
| TATAGGTACCTGTGAAGCAT+AGG | 0.569643 | 3.3:+92846998 | None:intergenic |
| AGTATTGATGCGAGCAACCG+TGG | 0.569989 | 3.3:-92845141 | MS.gene69442:CDS |
| ATCAATTGAACAAGTTGCAT+AGG | 0.570855 | 3.3:+92847279 | None:intergenic |
| AACATTACAGCGAAAAGGAA+AGG | 0.574650 | 3.3:+92844752 | None:intergenic |
| AACAGTTACGGTGGAAGTAG+CGG | 0.579345 | 3.3:-92839968 | MS.gene69442:CDS |
| TACTTGAGAGTTACAGTCCA+CGG | 0.579726 | 3.3:+92845124 | None:intergenic |
| GGCTTTGGTAATAACATCCA+TGG | 0.580963 | 3.3:+92843727 | None:intergenic |
| AGGCCAATGCCTGGAATGGT+TGG | 0.582117 | 3.3:-92835542 | MS.gene69442:CDS |
| TCAACATGGTCAACGAGAGA+TGG | 0.582556 | 3.3:-92846698 | MS.gene69442:CDS |
| AAGAGATCGGGGAGAGCTGG+AGG | 0.583017 | 3.3:-92835134 | MS.gene69442:CDS |
| AAGAAATGTCTCCTTGCAGA+AGG | 0.583352 | 3.3:-92845297 | MS.gene69442:CDS |
| AAGTACCAGGAAACAAATCC+TGG | 0.584309 | 3.3:-92844797 | MS.gene69442:CDS |
| ATTCTATTTCGTGCTACTAG+GGG | 0.585573 | 3.3:-92846076 | MS.gene69442:CDS |
| ATGATTATGGAAAATTGTGC+CGG | 0.587668 | 3.3:-92835740 | MS.gene69442:CDS |
| ATCCTAAATGGCATGGCACA+CGG | 0.589895 | 3.3:-92844269 | MS.gene69442:CDS |
| TAGATCGCCAAGGTAAGAGA+TGG | 0.589934 | 3.3:+92847230 | None:intergenic |
| AAAATGGTGCACTGGATCAC+AGG | 0.590090 | 3.3:-92843966 | MS.gene69442:CDS |
| GGCGTAGATCCTAAATGGCA+TGG | 0.594393 | 3.3:-92844276 | MS.gene69442:CDS |
| AATCAAAACCACCTGCATGT+AGG | 0.594477 | 3.3:+92844411 | None:intergenic |
| TTACTCAAGTCATCTGAGAA+GGG | 0.598455 | 3.3:+92845794 | None:intergenic |
| CATGAGGCCAATGCCTGGAA+TGG | 0.600183 | 3.3:-92835546 | MS.gene69442:CDS |
| AACAATTTCAGATGATGCAG+AGG | 0.601965 | 3.3:-92845772 | MS.gene69442:intron |
| ATTTGAAAGACAGCCTAACT+GGG | 0.604731 | 3.3:-92835095 | MS.gene69442:CDS |
| CCACGTGACTCTGATTCAAG+AGG | 0.607108 | 3.3:+92843546 | None:intergenic |
| CAGTAAAGCAGATAAAATTG+GGG | 0.611660 | 3.3:-92846147 | MS.gene69442:CDS |
| ATGACCGTGGAACGCACATG+AGG | 0.615692 | 3.3:-92835562 | MS.gene69442:CDS |
| GATGTCGCGATCCTACATGC+AGG | 0.623019 | 3.3:-92844422 | MS.gene69442:intron |
| GTGACAAGGTTGCAATGGAA+GGG | 0.623466 | 3.3:-92835809 | MS.gene69442:CDS |
| ACTTTGGAAAGAGGTTGAGA+AGG | 0.626635 | 3.3:+92839854 | None:intergenic |
| CGAACATAGGGAGTAGGTGA+AGG | 0.627364 | 3.3:-92840031 | MS.gene69442:intron |
| AACTCTTGTAGGATAAGAAG+AGG | 0.627476 | 3.3:-92835195 | MS.gene69442:intron |
| TGATTATGGAAAATTGTGCC+GGG | 0.629025 | 3.3:-92835739 | MS.gene69442:CDS |
| TTCAAACTTGGAGACATCGA+AGG | 0.637014 | 3.3:-92839942 | MS.gene69442:CDS |
| CAAATATATCTCCATCAACA+TGG | 0.637172 | 3.3:-92846712 | MS.gene69442:CDS |
| CAAATAGGTTAAAAGATGTG+GGG | 0.638472 | 3.3:-92846780 | MS.gene69442:intron |
| AAGAGAACCAGAAGAGATCG+GGG | 0.640560 | 3.3:-92835145 | MS.gene69442:CDS |
| GAAGCCACGTCATGAAAACA+TGG | 0.640897 | 3.3:-92835763 | MS.gene69442:CDS |
| GCTGTAATGTTTGGTGATTG+GGG | 0.649165 | 3.3:-92844740 | MS.gene69442:CDS |
| AAGATTATTGCTGATGACCG+TGG | 0.649545 | 3.3:-92835575 | MS.gene69442:CDS |
| CCCATTGTAGATCAAAGAGT+AGG | 0.649812 | 3.3:+92843575 | None:intergenic |
| ATCTTCTCTGAGACAAGCGA+AGG | 0.651215 | 3.3:-92837588 | MS.gene69442:intron |
| GTGATGATTGTGTACACACC+AGG | 0.653396 | 3.3:+92844779 | None:intergenic |
| GGACTGTAACTCTCAAGTAG+AGG | 0.654482 | 3.3:-92845120 | MS.gene69442:CDS |
| CAGGACACCAGCCTTCTGCA+AGG | 0.658702 | 3.3:+92845286 | None:intergenic |
| CTGTTCAAGAATACAATTTG+GGG | 0.666631 | 3.3:+92844022 | None:intergenic |
| AGTTACGGTGGAAGTAGCGG+TGG | 0.667128 | 3.3:-92839965 | MS.gene69442:CDS |
| AATGCCAACAATGAGAACAA+TGG | 0.667477 | 3.3:+92842602 | None:intergenic |
| GAAGATGAAAACAGTTACGG+TGG | 0.674861 | 3.3:-92839977 | MS.gene69442:CDS |
| CACAAACGAACATAGGGAGT+AGG | 0.674992 | 3.3:-92840037 | MS.gene69442:intron |
| GTCTCAGAGAAGATGCACAT+AGG | 0.676365 | 3.3:+92837597 | None:intergenic |
| CCTCTTGAATCAGAGTCACG+TGG | 0.695438 | 3.3:-92843546 | MS.gene69442:CDS |
| TGAGGAGTTTGATTTCAGCG+AGG | 0.723862 | 3.3:-92843508 | MS.gene69442:CDS |
| TCAAGAGACTGATCAACCTG+CGG | 0.732985 | 3.3:-92835056 | MS.gene69442:intron |
| CTACTCTTTGATCTACAATG+GGG | 0.740978 | 3.3:-92843574 | MS.gene69442:CDS |
| CGAAGGCAGAGAGATCGGTG+TGG | 0.763887 | 3.3:-92839925 | MS.gene69442:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 92834804 | 92847322 | 92834804 | ID=MS.gene69442 |
| chr3.3 | mRNA | 92834804 | 92847322 | 92834804 | ID=MS.gene69442.t1;Parent=MS.gene69442 |
| chr3.3 | exon | 92847209 | 92847322 | 92847209 | ID=MS.gene69442.t1.exon1;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92847209 | 92847322 | 92847209 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92847007 | 92847051 | 92847007 | ID=MS.gene69442.t1.exon2;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92847007 | 92847051 | 92847007 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92846673 | 92846795 | 92846673 | ID=MS.gene69442.t1.exon3;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92846673 | 92846795 | 92846673 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92846479 | 92846586 | 92846479 | ID=MS.gene69442.t1.exon4;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92846479 | 92846586 | 92846479 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92846270 | 92846380 | 92846270 | ID=MS.gene69442.t1.exon5;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92846270 | 92846380 | 92846270 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92846012 | 92846185 | 92846012 | ID=MS.gene69442.t1.exon6;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92846012 | 92846185 | 92846012 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92845773 | 92845909 | 92845773 | ID=MS.gene69442.t1.exon7;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92845773 | 92845909 | 92845773 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92845536 | 92845632 | 92845536 | ID=MS.gene69442.t1.exon8;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92845536 | 92845632 | 92845536 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92845269 | 92845376 | 92845269 | ID=MS.gene69442.t1.exon9;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92845269 | 92845376 | 92845269 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92845023 | 92845171 | 92845023 | ID=MS.gene69442.t1.exon10;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92845023 | 92845171 | 92845023 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92844673 | 92844826 | 92844673 | ID=MS.gene69442.t1.exon11;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92844673 | 92844826 | 92844673 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92844423 | 92844583 | 92844423 | ID=MS.gene69442.t1.exon12;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92844423 | 92844583 | 92844423 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92844141 | 92844347 | 92844141 | ID=MS.gene69442.t1.exon13;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92844141 | 92844347 | 92844141 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92843874 | 92844057 | 92843874 | ID=MS.gene69442.t1.exon14;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92843874 | 92844057 | 92843874 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92843699 | 92843782 | 92843699 | ID=MS.gene69442.t1.exon15;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92843699 | 92843782 | 92843699 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92843420 | 92843613 | 92843420 | ID=MS.gene69442.t1.exon16;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92843420 | 92843613 | 92843420 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92842712 | 92842771 | 92842712 | ID=MS.gene69442.t1.exon17;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92842712 | 92842771 | 92842712 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92842566 | 92842635 | 92842566 | ID=MS.gene69442.t1.exon18;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92842566 | 92842635 | 92842566 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92839856 | 92840044 | 92839856 | ID=MS.gene69442.t1.exon19;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92839856 | 92840044 | 92839856 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92837589 | 92837697 | 92837589 | ID=MS.gene69442.t1.exon20;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92837589 | 92837697 | 92837589 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92835698 | 92835831 | 92835698 | ID=MS.gene69442.t1.exon21;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92835698 | 92835831 | 92835698 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92835520 | 92835594 | 92835520 | ID=MS.gene69442.t1.exon22;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92835520 | 92835594 | 92835520 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92835057 | 92835206 | 92835057 | ID=MS.gene69442.t1.exon23;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92835057 | 92835206 | 92835057 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| chr3.3 | exon | 92834804 | 92834923 | 92834804 | ID=MS.gene69442.t1.exon24;Parent=MS.gene69442.t1 |
| chr3.3 | CDS | 92834804 | 92834923 | 92834804 | ID=cds.MS.gene69442.t1;Parent=MS.gene69442.t1 |
| Gene Sequence |
| Protein sequence |
