Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70675.t1 | XP_024634011.1 | 96.3 | 109 | 3 | 1 | 1 | 108 | 1 | 109 | 2.80E-53 | 217.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70675.t1 | A0A396IWG2 | 96.3 | 109 | 3 | 1 | 1 | 108 | 1 | 109 | 2.0e-53 | 217.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049373 | MS.gene70675 | 0.801735 | 7.83E-49 | -1.69E-46 |
| MS.gene049396 | MS.gene70675 | 0.805601 | 1.23E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene70675 | 0.810387 | 1.18E-50 | -1.69E-46 |
| MS.gene050502 | MS.gene70675 | 0.828949 | 6.76E-55 | -1.69E-46 |
| MS.gene05080 | MS.gene70675 | 0.802277 | 6.06E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene70675 | 0.824535 | 7.66E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene70675 | 0.827587 | 1.44E-54 | -1.69E-46 |
| MS.gene051838 | MS.gene70675 | 0.827573 | 1.45E-54 | -1.69E-46 |
| MS.gene051941 | MS.gene70675 | 0.834924 | 2.26E-56 | -1.69E-46 |
| MS.gene052164 | MS.gene70675 | 0.822506 | 2.28E-53 | -1.69E-46 |
| MS.gene052346 | MS.gene70675 | 0.810105 | 1.36E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene70675 | 0.82795 | 1.18E-54 | -1.69E-46 |
| MS.gene052503 | MS.gene70675 | 0.819207 | 1.31E-52 | -1.69E-46 |
| MS.gene052823 | MS.gene70675 | 0.8193 | 1.25E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene70675 | 0.810001 | 1.43E-50 | -1.69E-46 |
| MS.gene052966 | MS.gene70675 | 0.814196 | 1.74E-51 | -1.69E-46 |
| MS.gene053318 | MS.gene70675 | 0.801144 | 1.03E-48 | -1.69E-46 |
| MS.gene053436 | MS.gene70675 | 0.812637 | 3.84E-51 | -1.69E-46 |
| MS.gene053480 | MS.gene70675 | 0.80615 | 9.46E-50 | -1.69E-46 |
| MS.gene053626 | MS.gene70675 | 0.81128 | 7.57E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene70675 | 0.810246 | 1.27E-50 | -1.69E-46 |
| MS.gene053889 | MS.gene70675 | 0.821915 | 3.13E-53 | -1.69E-46 |
| MS.gene053916 | MS.gene70675 | 0.805513 | 1.29E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene70675 | 0.827182 | 1.80E-54 | -1.69E-46 |
| MS.gene055741 | MS.gene70675 | 0.839628 | 1.41E-57 | -1.69E-46 |
| MS.gene055744 | MS.gene70675 | 0.830832 | 2.35E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene70675 | 0.810503 | 1.12E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene70675 | 0.830885 | 2.28E-55 | -1.69E-46 |
| MS.gene055911 | MS.gene70675 | 0.828813 | 7.30E-55 | -1.69E-46 |
| MS.gene056166 | MS.gene70675 | 0.820612 | 6.26E-53 | -1.69E-46 |
| MS.gene056387 | MS.gene70675 | 0.820076 | 8.30E-53 | -1.69E-46 |
| MS.gene056446 | MS.gene70675 | 0.812384 | 4.36E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene70675 | 0.810316 | 1.22E-50 | -1.69E-46 |
| MS.gene056878 | MS.gene70675 | 0.801077 | 1.07E-48 | -1.69E-46 |
| MS.gene057019 | MS.gene70675 | 0.814602 | 1.42E-51 | -1.69E-46 |
| MS.gene057086 | MS.gene70675 | 0.833498 | 5.15E-56 | -1.69E-46 |
| MS.gene057289 | MS.gene70675 | 0.822803 | 1.95E-53 | -1.69E-46 |
| MS.gene057734 | MS.gene70675 | 0.822362 | 2.47E-53 | -1.69E-46 |
| MS.gene05787 | MS.gene70675 | 0.819897 | 9.12E-53 | -1.69E-46 |
| MS.gene058120 | MS.gene70675 | 0.823438 | 1.39E-53 | -1.69E-46 |
| MS.gene058948 | MS.gene70675 | 0.81982 | 9.50E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene70675 | 0.83376 | 4.43E-56 | -1.69E-46 |
| MS.gene059368 | MS.gene70675 | 0.822925 | 1.82E-53 | -1.69E-46 |
| MS.gene059517 | MS.gene70675 | 0.814404 | 1.57E-51 | -1.69E-46 |
| MS.gene059667 | MS.gene70675 | 0.831512 | 1.60E-55 | -1.69E-46 |
| MS.gene059680 | MS.gene70675 | 0.810728 | 9.98E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene70675 | 0.819145 | 1.35E-52 | -1.69E-46 |
| MS.gene060372 | MS.gene70675 | 0.826896 | 2.11E-54 | -1.69E-46 |
| MS.gene060778 | MS.gene70675 | 0.822057 | 2.90E-53 | -1.69E-46 |
| MS.gene060835 | MS.gene70675 | 0.804957 | 1.68E-49 | -1.69E-46 |
| MS.gene06097 | MS.gene70675 | 0.837424 | 5.24E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70675.t1 | MTR_3g076960 | 96.296 | 108 | 3 | 1 | 1 | 107 | 1 | 108 | 3.19e-70 | 207 |
| MS.gene70675.t1 | MTR_3g076950 | 68.908 | 119 | 23 | 6 | 1 | 108 | 1 | 116 | 8.28e-42 | 134 |
| MS.gene70675.t1 | MTR_3g076950 | 68.908 | 119 | 23 | 6 | 1 | 108 | 1 | 116 | 8.37e-42 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACCCTCTCAACGATTTC+AGG | 0.158944 | 3.3:+65426973 | None:intergenic |
| TTGAAGGCCATCTCACCATT+TGG | 0.332611 | 3.3:+65427060 | None:intergenic |
| GGTTGTGGTGGTGGCGGCCA+TGG | 0.367830 | 3.3:+65426995 | None:intergenic |
| CCTTCGTGGGTAATGTTGAT+TGG | 0.388716 | 3.3:+65426870 | None:intergenic |
| CGATTTCAGGGTTGTGGTGG+TGG | 0.397034 | 3.3:+65426986 | None:intergenic |
| TGAAGGCCATCTCACCATTT+GGG | 0.418823 | 3.3:+65427061 | None:intergenic |
| CAACCCTCTCAACGATTTCA+GGG | 0.425008 | 3.3:+65426974 | None:intergenic |
| CCATTTGACCTGTTCCTTCG+TGG | 0.432698 | 3.3:+65426856 | None:intergenic |
| ATAGACAACCCTCTTGTTGA+AGG | 0.438765 | 3.3:+65427044 | None:intergenic |
| GTCACTCATGGAATACATTC+AGG | 0.444691 | 3.3:-65426834 | MS.gene70675:CDS |
| CATTTGACCTGTTCCTTCGT+GGG | 0.467646 | 3.3:+65426857 | None:intergenic |
| CAACAAGAGGGTTGTCTATG+AGG | 0.468986 | 3.3:-65427040 | MS.gene70675:CDS |
| CCCTTCGAACTTCTGAAATG+TGG | 0.470828 | 3.3:+65427106 | None:intergenic |
| TCTCAACGATTTCAGGGTTG+TGG | 0.485866 | 3.3:+65426980 | None:intergenic |
| TTTCAGGGTTGTGGTGGTGG+CGG | 0.488542 | 3.3:+65426989 | None:intergenic |
| CGTTGAGAGGGTTGAAGTGG+TGG | 0.489360 | 3.3:-65426965 | MS.gene70675:CDS |
| AACATTACCCACGAAGGAAC+AGG | 0.491085 | 3.3:-65426864 | MS.gene70675:CDS |
| TATGAGGAGGATGCGATTGA+AGG | 0.492172 | 3.3:-65427024 | MS.gene70675:CDS |
| CCACGAAGGAACAGGTCAAA+TGG | 0.498880 | 3.3:-65426856 | MS.gene70675:CDS |
| GTACCTGAGGTTAGGCCCTA+TGG | 0.503566 | 3.3:-65426931 | MS.gene70675:CDS |
| CCACCATAGGGCCTAACCTC+AGG | 0.504816 | 3.3:+65426928 | None:intergenic |
| GAATACTCTCGCTACCCAAA+TGG | 0.533741 | 3.3:-65427075 | MS.gene70675:CDS |
| CCTTCGAACTTCTGAAATGT+GGG | 0.541962 | 3.3:+65427107 | None:intergenic |
| ATGAACGTGTACCTGAGGTT+AGG | 0.542173 | 3.3:-65426939 | MS.gene70675:CDS |
| CCCACATTTCAGAAGTTCGA+AGG | 0.543003 | 3.3:-65427107 | MS.gene70675:CDS |
| ACAGGTCAAATGGTCACTCA+TGG | 0.552434 | 3.3:-65426846 | MS.gene70675:CDS |
| CAACGATTTCAGGGTTGTGG+TGG | 0.564097 | 3.3:+65426983 | None:intergenic |
| GTAGATCACTTGACCACCAT+AGG | 0.569439 | 3.3:+65426915 | None:intergenic |
| AATGTGGGTGTGGTAGTCAT+TGG | 0.581040 | 3.3:+65427122 | None:intergenic |
| GGGTAGCGAGAGTATTCGCT+AGG | 0.581864 | 3.3:+65427081 | None:intergenic |
| AGTGATCTACGAAGAGGACG+TGG | 0.585316 | 3.3:-65426902 | MS.gene70675:CDS |
| TCGCTACCCAAATGGTGAGA+TGG | 0.585879 | 3.3:-65427067 | MS.gene70675:CDS |
| GTGAGATGGCCTTCAACAAG+AGG | 0.602330 | 3.3:-65427053 | MS.gene70675:CDS |
| TAGATCACTTGACCACCATA+GGG | 0.606041 | 3.3:+65426916 | None:intergenic |
| GAACTTCTGAAATGTGGGTG+TGG | 0.614497 | 3.3:+65427112 | None:intergenic |
| CCACATTTCAGAAGTTCGAA+GGG | 0.620439 | 3.3:-65427106 | MS.gene70675:CDS |
| CAACCCTGAAATCGTTGAGA+GGG | 0.621410 | 3.3:-65426977 | MS.gene70675:CDS |
| CCTGAGGTTAGGCCCTATGG+TGG | 0.626513 | 3.3:-65426928 | MS.gene70675:CDS |
| ACAACCCTGAAATCGTTGAG+AGG | 0.647655 | 3.3:-65426978 | MS.gene70675:CDS |
| CAAGAGGGTTGTCTATGAGG+AGG | 0.649483 | 3.3:-65427037 | MS.gene70675:CDS |
| GCGATTGAAGGATCACGCCA+TGG | 0.655981 | 3.3:-65427012 | MS.gene70675:CDS |
| TGAGATGGCCTTCAACAAGA+GGG | 0.673455 | 3.3:-65427052 | MS.gene70675:CDS |
| TGGTCAAGTGATCTACGAAG+AGG | 0.694239 | 3.3:-65426908 | MS.gene70675:CDS |
| AATCGTTGAGAGGGTTGAAG+TGG | 0.698905 | 3.3:-65426968 | MS.gene70675:CDS |
| CCAATCAACATTACCCACGA+AGG | 0.702298 | 3.3:-65426870 | MS.gene70675:CDS |
| GGAATATGAACGTGTACCTG+AGG | 0.717370 | 3.3:-65426944 | MS.gene70675:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAGACAACCCTCTTGTTGA+AGG | + | chr3.3:65426919-65426938 | None:intergenic | 40.0% | |
| CCACATTTCAGAAGTTCGAA+GGG | - | chr3.3:65426854-65426873 | MS.gene70675:CDS | 40.0% | |
| GTCACTCATGGAATACATTC+AGG | - | chr3.3:65427126-65427145 | MS.gene70675:CDS | 40.0% | |
| TAGATCACTTGACCACCATA+GGG | + | chr3.3:65427047-65427066 | None:intergenic | 40.0% | |
| !! | CCTTCGAACTTCTGAAATGT+GGG | + | chr3.3:65426856-65426875 | None:intergenic | 40.0% |
| AACATTACCCACGAAGGAAC+AGG | - | chr3.3:65427096-65427115 | MS.gene70675:CDS | 45.0% | |
| AATCGTTGAGAGGGTTGAAG+TGG | - | chr3.3:65426992-65427011 | MS.gene70675:CDS | 45.0% | |
| AATGTGGGTGTGGTAGTCAT+TGG | + | chr3.3:65426841-65426860 | None:intergenic | 45.0% | |
| ACAACCCTGAAATCGTTGAG+AGG | - | chr3.3:65426982-65427001 | MS.gene70675:CDS | 45.0% | |
| ACAGGTCAAATGGTCACTCA+TGG | - | chr3.3:65427114-65427133 | MS.gene70675:CDS | 45.0% | |
| ATGAACGTGTACCTGAGGTT+AGG | - | chr3.3:65427021-65427040 | MS.gene70675:CDS | 45.0% | |
| CAACAAGAGGGTTGTCTATG+AGG | - | chr3.3:65426920-65426939 | MS.gene70675:CDS | 45.0% | |
| CAACCCTCTCAACGATTTCA+GGG | + | chr3.3:65426989-65427008 | None:intergenic | 45.0% | |
| CAACCCTGAAATCGTTGAGA+GGG | - | chr3.3:65426983-65427002 | MS.gene70675:CDS | 45.0% | |
| CATTTGACCTGTTCCTTCGT+GGG | + | chr3.3:65427106-65427125 | None:intergenic | 45.0% | |
| CCAATCAACATTACCCACGA+AGG | - | chr3.3:65427090-65427109 | MS.gene70675:CDS | 45.0% | |
| CCCACATTTCAGAAGTTCGA+AGG | - | chr3.3:65426853-65426872 | MS.gene70675:CDS | 45.0% | |
| CCTTCGTGGGTAATGTTGAT+TGG | + | chr3.3:65427093-65427112 | None:intergenic | 45.0% | |
| GAATACTCTCGCTACCCAAA+TGG | - | chr3.3:65426885-65426904 | MS.gene70675:CDS | 45.0% | |
| GGAATATGAACGTGTACCTG+AGG | - | chr3.3:65427016-65427035 | MS.gene70675:CDS | 45.0% | |
| GTAGATCACTTGACCACCAT+AGG | + | chr3.3:65427048-65427067 | None:intergenic | 45.0% | |
| TATGAGGAGGATGCGATTGA+AGG | - | chr3.3:65426936-65426955 | MS.gene70675:CDS | 45.0% | |
| TCAACCCTCTCAACGATTTC+AGG | + | chr3.3:65426990-65427009 | None:intergenic | 45.0% | |
| TCTCAACGATTTCAGGGTTG+TGG | + | chr3.3:65426983-65427002 | None:intergenic | 45.0% | |
| TGAAGGCCATCTCACCATTT+GGG | + | chr3.3:65426902-65426921 | None:intergenic | 45.0% | |
| TGAGATGGCCTTCAACAAGA+GGG | - | chr3.3:65426908-65426927 | MS.gene70675:CDS | 45.0% | |
| TGGTCAAGTGATCTACGAAG+AGG | - | chr3.3:65427052-65427071 | MS.gene70675:CDS | 45.0% | |
| TTGAAGGCCATCTCACCATT+TGG | + | chr3.3:65426903-65426922 | None:intergenic | 45.0% | |
| !! | CCCTTCGAACTTCTGAAATG+TGG | + | chr3.3:65426857-65426876 | None:intergenic | 45.0% |
| !! | GAACTTCTGAAATGTGGGTG+TGG | + | chr3.3:65426851-65426870 | None:intergenic | 45.0% |
| AGTGATCTACGAAGAGGACG+TGG | - | chr3.3:65427058-65427077 | MS.gene70675:CDS | 50.0% | |
| CAACGATTTCAGGGTTGTGG+TGG | + | chr3.3:65426980-65426999 | None:intergenic | 50.0% | |
| CAAGAGGGTTGTCTATGAGG+AGG | - | chr3.3:65426923-65426942 | MS.gene70675:CDS | 50.0% | |
| CCACGAAGGAACAGGTCAAA+TGG | - | chr3.3:65427104-65427123 | MS.gene70675:CDS | 50.0% | |
| CCATTTGACCTGTTCCTTCG+TGG | + | chr3.3:65427107-65427126 | None:intergenic | 50.0% | |
| GTGAGATGGCCTTCAACAAG+AGG | - | chr3.3:65426907-65426926 | MS.gene70675:CDS | 50.0% | |
| TCGCTACCCAAATGGTGAGA+TGG | - | chr3.3:65426893-65426912 | MS.gene70675:CDS | 50.0% | |
| CGATTTCAGGGTTGTGGTGG+TGG | + | chr3.3:65426977-65426996 | None:intergenic | 55.0% | |
| CGTTGAGAGGGTTGAAGTGG+TGG | - | chr3.3:65426995-65427014 | MS.gene70675:CDS | 55.0% | |
| GCGATTGAAGGATCACGCCA+TGG | - | chr3.3:65426948-65426967 | MS.gene70675:CDS | 55.0% | |
| GTACCTGAGGTTAGGCCCTA+TGG | - | chr3.3:65427029-65427048 | MS.gene70675:CDS | 55.0% | |
| !! | GGGTAGCGAGAGTATTCGCT+AGG | + | chr3.3:65426882-65426901 | None:intergenic | 55.0% |
| !! | TTTCAGGGTTGTGGTGGTGG+CGG | + | chr3.3:65426974-65426993 | None:intergenic | 55.0% |
| CCACCATAGGGCCTAACCTC+AGG | + | chr3.3:65427035-65427054 | None:intergenic | 60.0% | |
| CCTGAGGTTAGGCCCTATGG+TGG | - | chr3.3:65427032-65427051 | MS.gene70675:CDS | 60.0% | |
| !! | GGTTGTGGTGGTGGCGGCCA+TGG | + | chr3.3:65426968-65426987 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 65426828 | 65427154 | 65426828 | ID=MS.gene70675 |
| chr3.3 | mRNA | 65426828 | 65427154 | 65426828 | ID=MS.gene70675.t1;Parent=MS.gene70675 |
| chr3.3 | exon | 65426828 | 65427154 | 65426828 | ID=MS.gene70675.t1.exon1;Parent=MS.gene70675.t1 |
| chr3.3 | CDS | 65426828 | 65427154 | 65426828 | ID=cds.MS.gene70675.t1;Parent=MS.gene70675.t1 |
| Gene Sequence |
| Protein sequence |
