Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71353.t1 | XP_003621186.2 | 97.7 | 258 | 6 | 0 | 1 | 258 | 46 | 303 | 1.70E-136 | 495.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71353.t1 | P81392 | 50.7 | 270 | 106 | 8 | 1 | 258 | 46 | 300 | 1.2e-55 | 218.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71353.t1 | Q2HVT2 | 97.7 | 258 | 6 | 0 | 1 | 258 | 46 | 303 | 1.2e-136 | 495.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene71353 | 0.813789 | 2.14E-51 | -1.69E-46 |
| MS.gene049153 | MS.gene71353 | 0.80141 | 9.12E-49 | -1.69E-46 |
| MS.gene049346 | MS.gene71353 | 0.80267 | 5.03E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene71353 | 0.813924 | 2.00E-51 | -1.69E-46 |
| MS.gene050128 | MS.gene71353 | 0.805076 | 1.59E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene71353 | 0.80794 | 3.95E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene71353 | 0.804392 | 2.21E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene71353 | 0.817201 | 3.73E-52 | -1.69E-46 |
| MS.gene050398 | MS.gene71353 | 0.803788 | 2.95E-49 | -1.69E-46 |
| MS.gene050406 | MS.gene71353 | 0.803375 | 3.59E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene71353 | 0.806851 | 6.73E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene71353 | 0.806921 | 6.50E-50 | -1.69E-46 |
| MS.gene051983 | MS.gene71353 | 0.820986 | 5.13E-53 | -1.69E-46 |
| MS.gene052077 | MS.gene71353 | 0.809238 | 2.09E-50 | -1.69E-46 |
| MS.gene052178 | MS.gene71353 | 0.805842 | 1.10E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene71353 | 0.818515 | 1.88E-52 | -1.69E-46 |
| MS.gene053073 | MS.gene71353 | 0.802308 | 5.97E-49 | -1.69E-46 |
| MS.gene053204 | MS.gene71353 | 0.81117 | 8.00E-51 | -1.69E-46 |
| MS.gene053557 | MS.gene71353 | 0.812964 | 3.25E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene71353 | 0.832068 | 1.17E-55 | -1.69E-46 |
| MS.gene053928 | MS.gene71353 | 0.827121 | 1.86E-54 | -1.69E-46 |
| MS.gene054010 | MS.gene71353 | 0.812329 | 4.48E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene71353 | 0.82949 | 5.00E-55 | -1.69E-46 |
| MS.gene054012 | MS.gene71353 | 0.828415 | 9.11E-55 | -1.69E-46 |
| MS.gene054013 | MS.gene71353 | 0.826048 | 3.36E-54 | -1.69E-46 |
| MS.gene055428 | MS.gene71353 | 0.801898 | 7.24E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene71353 | 0.851223 | 1.02E-60 | -1.69E-46 |
| MS.gene056979 | MS.gene71353 | 0.808388 | 3.17E-50 | -1.69E-46 |
| MS.gene05702 | MS.gene71353 | 0.809941 | 1.48E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene71353 | 0.803702 | 3.07E-49 | -1.69E-46 |
| MS.gene057752 | MS.gene71353 | 0.803893 | 2.81E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene71353 | 0.830981 | 2.16E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene71353 | 0.807989 | 3.86E-50 | -1.69E-46 |
| MS.gene058142 | MS.gene71353 | 0.839547 | 1.48E-57 | -1.69E-46 |
| MS.gene05907 | MS.gene71353 | 0.811953 | 5.41E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene71353 | 0.818843 | 1.59E-52 | -1.69E-46 |
| MS.gene059894 | MS.gene71353 | 0.810752 | 9.86E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene71353 | 0.845278 | 4.49E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATAGCTCGTTTACTAAA+AGG | 0.252975 | 7.2:-87269229 | MS.gene71353:CDS |
| CTTCTTTCCCATTGACCTCT+TGG | 0.294000 | 7.2:+87269412 | None:intergenic |
| GAAACATTTGAATCTTTGTT+TGG | 0.358400 | 7.2:-87269091 | MS.gene71353:CDS |
| ATGTCATTATCTGTTCTTTG+TGG | 0.377477 | 7.2:+87269547 | None:intergenic |
| GTACTAACCGATGATTTGAT+TGG | 0.381105 | 7.2:+87269193 | None:intergenic |
| GAAAATGAGTTACTATTTGT+AGG | 0.397920 | 7.2:+87269301 | None:intergenic |
| GCAAAAGTTGCAGACTTAGA+TGG | 0.403906 | 7.2:-87270138 | MS.gene71353:CDS |
| TTCCTAAAAGATCTTGAAGA+TGG | 0.422826 | 7.2:+87270050 | None:intergenic |
| AGATTGACCTGGTTCTTTAG+TGG | 0.427373 | 7.2:+87269276 | None:intergenic |
| CCATTTCTCAAGCAAAGAAA+AGG | 0.447906 | 7.2:+87268949 | None:intergenic |
| TCTTGTAAAACCGTTGCGTC+TGG | 0.450422 | 7.2:+87269007 | None:intergenic |
| AAGAATTCCAAGAGGTCAAT+GGG | 0.456644 | 7.2:-87269419 | MS.gene71353:CDS |
| ACCTAAGGCCAGGAATCAAA+AGG | 0.458436 | 7.2:-87270108 | MS.gene71353:CDS |
| AAAGAATTCCAAGAGGTCAA+TGG | 0.463998 | 7.2:-87269420 | MS.gene71353:CDS |
| AAAATGAGTTACTATTTGTA+GGG | 0.472669 | 7.2:+87269302 | None:intergenic |
| AGCAGAATCAAAGTTGTTGA+AGG | 0.476547 | 7.2:+87269147 | None:intergenic |
| CATTATCTGTTCTTTGTGGA+AGG | 0.487830 | 7.2:+87269551 | None:intergenic |
| ATTGATTCTGAACCAACATC+AGG | 0.495731 | 7.2:+87268974 | None:intergenic |
| TTTGATTCTGCTTCTAGTAG+TGG | 0.508963 | 7.2:-87269136 | MS.gene71353:CDS |
| AAGCTCAAGAAATCACAAAC+AGG | 0.513721 | 7.2:-87269493 | MS.gene71353:CDS |
| TAGCTCGTTTACTAAAAGGT+TGG | 0.524179 | 7.2:-87269225 | MS.gene71353:CDS |
| CCTAAGGCCAGGAATCAAAA+GGG | 0.528003 | 7.2:-87270107 | MS.gene71353:CDS |
| TGGACTAATTACCTAAGGCC+AGG | 0.531588 | 7.2:-87270118 | MS.gene71353:CDS |
| TGCTTCTAGTAGTGGAACAA+AGG | 0.546510 | 7.2:-87269128 | MS.gene71353:CDS |
| ACTTCAAACAGATATTCAAA+TGG | 0.548282 | 7.2:-87269389 | MS.gene71353:CDS |
| ATCTTTGTCACCAGACGCAA+CGG | 0.551917 | 7.2:-87269017 | MS.gene71353:CDS |
| CATTTCTCAAGCAAAGAAAA+GGG | 0.562614 | 7.2:+87268950 | None:intergenic |
| GCTTCTAGTAGTGGAACAAA+GGG | 0.568848 | 7.2:-87269127 | MS.gene71353:CDS |
| TTAGATGGACTAATTACCTA+AGG | 0.574462 | 7.2:-87270123 | MS.gene71353:CDS |
| GATGAAAGTAAGCCTGATGT+TGG | 0.577667 | 7.2:-87268986 | MS.gene71353:CDS |
| GGGTAACTTCACTGAGCAAG+AGG | 0.593302 | 7.2:-87270086 | MS.gene71353:CDS |
| GATGATTCCAAAGACTCCAA+AGG | 0.605819 | 7.2:+87269061 | None:intergenic |
| CTAAGGCCAGGAATCAAAAG+GGG | 0.682983 | 7.2:-87270106 | MS.gene71353:CDS |
| GCATCTCAAAGAATTCCAAG+AGG | 0.704482 | 7.2:-87269427 | MS.gene71353:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAACTACTAAAAAAAT+TGG | + | chr7.2:87269446-87269465 | None:intergenic | 10.0% |
| !! | ATACAAAAAATTATAGAAAT+AGG | + | chr7.2:87269305-87269324 | None:intergenic | 10.0% |
| !! | TACAAAAAATTATAGAAATA+GGG | + | chr7.2:87269304-87269323 | None:intergenic | 10.0% |
| !!! | ATTTTTTTAGTAGTTTTTTT+TGG | - | chr7.2:87269446-87269465 | MS.gene71353:CDS | 10.0% |
| !! | ATAATGACATAAAAAACTAT+TGG | - | chr7.2:87269559-87269578 | MS.gene71353:CDS | 15.0% |
| !! | TTATTCAAATTATGAAGAAA+AGG | + | chr7.2:87269078-87269097 | None:intergenic | 15.0% |
| !! | TTTAAATATCCCTAAAAAAA+AGG | - | chr7.2:87269346-87269365 | MS.gene71353:CDS | 15.0% |
| !!! | AAATTAGAAGATAATTTTTG+TGG | + | chr7.2:87269759-87269778 | None:intergenic | 15.0% |
| !! | AAAATGAGTTACTATTTGTA+GGG | + | chr7.2:87269794-87269813 | None:intergenic | 20.0% |
| !! | ATCTTTATGGAATTAAAAAC+AGG | + | chr7.2:87269224-87269243 | None:intergenic | 20.0% |
| !! | TCATAACTAGTATATCTTTA+TGG | + | chr7.2:87269237-87269256 | None:intergenic | 20.0% |
| !! | TTATGAAAGAATATAAGTGT+TGG | - | chr7.2:87269251-87269270 | MS.gene71353:CDS | 20.0% |
| !!! | AGCATTTGATTTCATTTTTT+AGG | - | chr7.2:87269148-87269167 | MS.gene71353:CDS | 20.0% |
| !!! | ATTAAATTTAGGACATAAGT+TGG | - | chr7.2:87269188-87269207 | MS.gene71353:CDS | 20.0% |
| !!! | ATTAGCAAATCCTTTTTTTT+AGG | + | chr7.2:87269359-87269378 | None:intergenic | 20.0% |
| !!! | TTAGCAAATCCTTTTTTTTA+GGG | + | chr7.2:87269358-87269377 | None:intergenic | 20.0% |
| ! | AACATTTATGATGTAACAGA+TGG | - | chr7.2:87269502-87269521 | MS.gene71353:CDS | 25.0% |
| ! | ACATTTATGATGTAACAGAT+GGG | - | chr7.2:87269503-87269522 | MS.gene71353:CDS | 25.0% |
| ! | ACTTCAAACAGATATTCAAA+TGG | - | chr7.2:87269704-87269723 | MS.gene71353:intron | 25.0% |
| ! | AGGGATATTTAAATCCATTT+AGG | + | chr7.2:87269339-87269358 | None:intergenic | 25.0% |
| ! | GAAAAATCCAATCAAATCAT+CGG | - | chr7.2:87269893-87269912 | MS.gene71353:intron | 25.0% |
| ! | GAAAATGAGTTACTATTTGT+AGG | + | chr7.2:87269795-87269814 | None:intergenic | 25.0% |
| ! | GAAACATTTGAATCTTTGTT+TGG | - | chr7.2:87270002-87270021 | MS.gene71353:intron | 25.0% |
| ! | TAGGGACACAAATTAAAAAT+AGG | + | chr7.2:87269286-87269305 | None:intergenic | 25.0% |
| ! | TATATTAGACTTGTCCTAAA+TGG | - | chr7.2:87269322-87269341 | MS.gene71353:CDS | 25.0% |
| !!! | TTCAAGATCTTTTAGGAAAT+AGG | - | chr7.2:87269048-87269067 | MS.gene71353:CDS | 25.0% |
| AACATAGCTCGTTTACTAAA+AGG | - | chr7.2:87269864-87269883 | MS.gene71353:intron | 30.0% | |
| ATGTCATTATCTGTTCTTTG+TGG | + | chr7.2:87269549-87269568 | None:intergenic | 30.0% | |
| CATTTCTCAAGCAAAGAAAA+GGG | + | chr7.2:87270146-87270165 | None:intergenic | 30.0% | |
| TTATGATTCACATGTCTAAC+TGG | - | chr7.2:87269477-87269496 | MS.gene71353:CDS | 30.0% | |
| TTCCTAAAAGATCTTGAAGA+TGG | + | chr7.2:87269046-87269065 | None:intergenic | 30.0% | |
| ! | ATCCATCTTCAAGATCTTTT+AGG | - | chr7.2:87269041-87269060 | MS.gene71353:CDS | 30.0% |
| ! | CTTTTCTTTGCTTGAGAAAT+GGG | - | chr7.2:87270145-87270164 | MS.gene71353:CDS | 30.0% |
| !! | TTAGATGGACTAATTACCTA+AGG | - | chr7.2:87268970-87268989 | MS.gene71353:CDS | 30.0% |
| !!! | TGCTTCATTAAGTGCTTTTT+TGG | + | chr7.2:87269730-87269749 | None:intergenic | 30.0% |
| AAAGAATTCCAAGAGGTCAA+TGG | - | chr7.2:87269673-87269692 | MS.gene71353:intron | 35.0% | |
| AAGAATTCCAAGAGGTCAAT+GGG | - | chr7.2:87269674-87269693 | MS.gene71353:intron | 35.0% | |
| AAGCTCAAGAAATCACAAAC+AGG | - | chr7.2:87269600-87269619 | MS.gene71353:intron | 35.0% | |
| AGCAGAATCAAAGTTGTTGA+AGG | + | chr7.2:87269949-87269968 | None:intergenic | 35.0% | |
| CATTATCTGTTCTTTGTGGA+AGG | + | chr7.2:87269545-87269564 | None:intergenic | 35.0% | |
| CCATTTCTCAAGCAAAGAAA+AGG | + | chr7.2:87270147-87270166 | None:intergenic | 35.0% | |
| GTACTAACCGATGATTTGAT+TGG | + | chr7.2:87269903-87269922 | None:intergenic | 35.0% | |
| TAGCTCGTTTACTAAAAGGT+TGG | - | chr7.2:87269868-87269887 | MS.gene71353:intron | 35.0% | |
| TTTGATTCTGCTTCTAGTAG+TGG | - | chr7.2:87269957-87269976 | MS.gene71353:intron | 35.0% | |
| ! | ATTGATTCTGAACCAACATC+AGG | + | chr7.2:87270122-87270141 | None:intergenic | 35.0% |
| ! | CCTTTTCTTTGCTTGAGAAA+TGG | - | chr7.2:87270144-87270163 | MS.gene71353:CDS | 35.0% |
| ! | TTTTCTTCCACTAAAGAACC+AGG | - | chr7.2:87269810-87269829 | MS.gene71353:intron | 35.0% |
| !!! | TTTGTTTGGTTTTGAGCCTT+TGG | - | chr7.2:87270016-87270035 | MS.gene71353:intron | 35.0% |
| GATGAAAGTAAGCCTGATGT+TGG | - | chr7.2:87270107-87270126 | MS.gene71353:CDS | 40.0% | |
| GATGATTCCAAAGACTCCAA+AGG | + | chr7.2:87270035-87270054 | None:intergenic | 40.0% | |
| GCAAAAGTTGCAGACTTAGA+TGG | - | chr7.2:87268955-87268974 | MS.gene71353:CDS | 40.0% | |
| GCATAGCAAAAAGATTGACC+TGG | + | chr7.2:87269831-87269850 | None:intergenic | 40.0% | |
| GCATCTCAAAGAATTCCAAG+AGG | - | chr7.2:87269666-87269685 | MS.gene71353:intron | 40.0% | |
| ! | AGATTGACCTGGTTCTTTAG+TGG | + | chr7.2:87269820-87269839 | None:intergenic | 40.0% |
| ! | GCTTCTAGTAGTGGAACAAA+GGG | - | chr7.2:87269966-87269985 | MS.gene71353:intron | 40.0% |
| ! | TGCTTCTAGTAGTGGAACAA+AGG | - | chr7.2:87269965-87269984 | MS.gene71353:intron | 40.0% |
| !! | AAGTTACCCCTTTTGATTCC+TGG | + | chr7.2:87268996-87269015 | None:intergenic | 40.0% |
| !!! | TTTTGAGCCTTTGGAGTCTT+TGG | - | chr7.2:87270025-87270044 | MS.gene71353:intron | 40.0% |
| ACCTAAGGCCAGGAATCAAA+AGG | - | chr7.2:87268985-87269004 | MS.gene71353:CDS | 45.0% | |
| ATCTTTGTCACCAGACGCAA+CGG | - | chr7.2:87270076-87270095 | MS.gene71353:CDS | 45.0% | |
| CCTAAGGCCAGGAATCAAAA+GGG | - | chr7.2:87268986-87269005 | MS.gene71353:CDS | 45.0% | |
| CTAAGGCCAGGAATCAAAAG+GGG | - | chr7.2:87268987-87269006 | MS.gene71353:CDS | 45.0% | |
| CTTCTTTCCCATTGACCTCT+TGG | + | chr7.2:87269684-87269703 | None:intergenic | 45.0% | |
| TCTTGTAAAACCGTTGCGTC+TGG | + | chr7.2:87270089-87270108 | None:intergenic | 45.0% | |
| TGGACTAATTACCTAAGGCC+AGG | - | chr7.2:87268975-87268994 | MS.gene71353:CDS | 45.0% | |
| ! | CTTGAGAAATGGGCTTCTTG+AGG | - | chr7.2:87270155-87270174 | MS.gene71353:CDS | 45.0% |
| !!! | AAATTTAATTTTACAAAAAT+TGG | + | chr7.2:87269177-87269196 | None:intergenic | 5.0% |
| !!! | ATTTTTGTAAAATTAAATTT+AGG | - | chr7.2:87269177-87269196 | MS.gene71353:CDS | 5.0% |
| GGGTAACTTCACTGAGCAAG+AGG | - | chr7.2:87269007-87269026 | MS.gene71353:CDS | 50.0% | |
| !! | CCCTTTTGATTCCTGGCCTT+AGG | + | chr7.2:87268989-87269008 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 87268940 | 87270175 | 87268940 | ID=MS.gene71353 |
| chr7.2 | mRNA | 87268940 | 87270175 | 87268940 | ID=MS.gene71353.t1;Parent=MS.gene71353 |
| chr7.2 | exon | 87270046 | 87270175 | 87270046 | ID=MS.gene71353.t1.exon1;Parent=MS.gene71353.t1 |
| chr7.2 | CDS | 87270046 | 87270175 | 87270046 | ID=cds.MS.gene71353.t1;Parent=MS.gene71353.t1 |
| chr7.2 | exon | 87268940 | 87269594 | 87268940 | ID=MS.gene71353.t1.exon2;Parent=MS.gene71353.t1 |
| chr7.2 | CDS | 87268940 | 87269594 | 87268940 | ID=cds.MS.gene71353.t1;Parent=MS.gene71353.t1 |
| Gene Sequence |
| Protein sequence |
