AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72126

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72126.t1 MTR_8g098415 96.474 312 11 0 1 312 1 312 0.0 624
MS.gene72126.t1 MTR_8g098415 96.429 308 11 0 1 308 1 308 0.0 617
MS.gene72126.t1 MTR_8g098415 96.471 255 9 0 58 312 1 255 0.0 512
MS.gene72126.t1 MTR_1g100305 68.810 311 97 0 1 311 1 311 7.38e-163 456
MS.gene72126.t1 MTR_1g100395 68.790 314 98 0 1 314 1 314 9.09e-163 456
MS.gene72126.t1 MTR_1g100315 69.775 311 94 0 1 311 1 311 2.08e-161 452
MS.gene72126.t1 MTR_1g100335 68.489 311 98 0 1 311 1 311 5.52e-158 444
MS.gene72126.t1 MTR_8g098415 95.434 219 10 0 1 219 1 219 1.74e-154 431
MS.gene72126.t1 MTR_6g053550 65.359 306 103 2 9 311 17 322 5.50e-145 411
MS.gene72126.t1 MTR_1g068970 57.655 307 130 0 5 311 10 316 2.51e-129 371
MS.gene72126.t1 MTR_7g099850 57.792 308 129 1 5 311 10 317 2.99e-129 371
MS.gene72126.t1 MTR_1g100335 69.531 256 72 1 1 256 1 250 2.09e-126 362
MS.gene72126.t1 MTR_1g100335 72.917 240 65 0 1 240 1 240 7.63e-126 361
MS.gene72126.t1 MTR_6g053600 59.862 289 112 2 10 295 4 291 2.02e-120 348
MS.gene72126.t1 MTR_1g068970 57.025 242 104 0 70 311 8 249 2.24e-97 288
MS.gene72126.t1 MTR_3g077410 45.455 308 164 2 2 305 7 314 8.70e-94 281
MS.gene72126.t1 MTR_8g099995 45.659 311 165 3 2 308 7 317 6.01e-91 274
MS.gene72126.t1 MTR_4g105310 44.481 308 167 2 2 305 18 325 7.57e-88 267
MS.gene72126.t1 MTR_4g105310 46.091 243 128 1 2 241 18 260 6.50e-70 218
MS.gene72126.t1 MTR_6g081020 31.894 301 180 6 30 311 62 356 1.98e-30 118
MS.gene72126.t1 MTR_3g091070 31.561 301 158 11 32 311 43 316 6.97e-24 99.8
MS.gene72126.t1 MTR_6g041980 28.462 260 170 6 32 289 69 314 1.49e-23 99.4
MS.gene72126.t1 MTR_2g079710 33.025 324 158 13 31 311 88 395 4.77e-23 98.6
MS.gene72126.t1 MTR_3g009190 33.010 309 161 13 32 311 65 356 8.11e-23 97.4
MS.gene72126.t1 MTR_3g009190 33.010 309 161 13 32 311 83 374 9.43e-23 97.4
MS.gene72126.t1 MTR_6g453040 32.686 309 162 13 32 311 43 334 1.61e-22 96.3
MS.gene72126.t1 MTR_1g100345 47.945 73 38 0 33 105 4 76 1.36e-20 84.7
MS.gene72126.t1 MTR_3g009190 32.468 308 162 13 33 311 65 355 2.96e-20 90.5
MS.gene72126.t1 MTR_6g041980 27.473 182 125 2 32 213 69 243 1.40e-16 78.6
MS.gene72126.t1 MTR_3g098470 28.328 293 147 12 32 285 37 305 1.01e-14 73.9
MS.gene72126.t1 MTR_8g022500 33.628 113 50 1 34 146 431 518 2.68e-12 67.4
MS.gene72126.t1 MTR_6g453040 32.227 211 106 8 32 220 65 260 8.87e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72126.t1 AT4G11410 69.231 312 96 0 1 312 1 312 4.23e-164 459
MS.gene72126.t1 AT4G23430 68.051 313 99 1 1 312 1 313 3.78e-162 454
MS.gene72126.t1 AT4G23420 68.354 316 93 3 1 312 1 313 1.12e-161 453
MS.gene72126.t1 AT4G23420 68.354 316 93 3 1 312 1 313 1.12e-161 453
MS.gene72126.t1 AT4G23430 67.732 313 98 2 1 312 1 311 4.01e-159 447
MS.gene72126.t1 AT4G23420 68.404 307 90 3 10 312 27 330 3.33e-156 440
MS.gene72126.t1 AT4G23430 65.176 313 98 2 1 312 1 303 4.70e-152 429
MS.gene72126.t1 AT4G23430 64.856 313 97 3 1 312 1 301 3.18e-149 421
MS.gene72126.t1 AT4G23420 69.434 265 74 3 1 261 1 262 9.23e-134 380
MS.gene72126.t1 AT4G23420 69.804 255 72 2 1 255 1 250 3.25e-131 374
MS.gene72126.t1 AT4G11410 71.545 246 70 0 1 246 1 246 2.24e-130 372
MS.gene72126.t1 AT2G37540 55.375 307 137 0 5 311 9 315 1.18e-123 357
MS.gene72126.t1 AT5G02540 56.678 307 133 0 5 311 9 315 1.63e-119 347
MS.gene72126.t1 AT2G37540 58.577 239 99 0 5 243 9 247 2.98e-99 293
MS.gene72126.t1 AT4G24050 45.253 316 170 2 2 314 7 322 2.63e-91 275
MS.gene72126.t1 AT5G50130 47.134 314 162 3 2 311 7 320 7.16e-91 274
MS.gene72126.t1 AT5G50130 46.595 279 148 1 34 311 8 286 1.05e-81 249
MS.gene72126.t1 AT5G50130 46.786 280 147 2 34 311 8 287 6.86e-81 248
MS.gene72126.t1 AT4G24050 45.387 271 143 3 2 269 7 275 8.14e-74 228
MS.gene72126.t1 AT5G50130 45.756 271 143 3 2 268 7 277 8.36e-74 230
MS.gene72126.t1 AT1G64590 45.085 295 159 2 17 308 22 316 8.50e-74 230
MS.gene72126.t1 AT5G50130 45.956 272 142 4 2 268 7 278 6.65e-73 228
MS.gene72126.t1 AT5G50130 48.374 246 124 2 2 244 7 252 2.06e-72 224
MS.gene72126.t1 AT5G50130 48.583 247 123 3 2 244 7 253 1.51e-71 222
MS.gene72126.t1 AT5G50130 45.148 237 128 2 34 268 8 244 1.11e-63 203
MS.gene72126.t1 AT5G50130 45.378 238 127 3 34 268 8 245 6.37e-63 201
MS.gene72126.t1 AT4G27760 31.250 304 176 8 30 311 58 350 3.08e-32 123
MS.gene72126.t1 AT5G53090 30.000 300 185 7 30 311 57 349 3.83e-29 115
MS.gene72126.t1 AT1G03630 33.016 315 169 11 31 311 89 395 8.01e-26 106
MS.gene72126.t1 AT1G03630 33.016 315 169 11 31 311 91 397 8.44e-26 106
MS.gene72126.t1 AT5G53100 31.788 302 180 6 30 311 45 340 1.74e-24 102
MS.gene72126.t1 AT4G27440 31.268 339 184 13 13 311 68 397 7.11e-24 100
MS.gene72126.t1 AT4G27440 31.268 339 184 13 13 311 68 397 7.11e-24 100
MS.gene72126.t1 AT5G54190 29.524 315 177 11 33 311 96 401 9.00e-24 100
MS.gene72126.t1 AT5G04070 30.033 303 172 11 32 309 61 348 1.72e-23 99.4
MS.gene72126.t1 AT4G09750 27.413 259 174 6 32 289 46 291 2.17e-23 98.6
MS.gene72126.t1 AT5G15940 31.579 304 172 12 32 311 63 354 2.06e-22 96.3
MS.gene72126.t1 AT5G15940 31.579 304 172 12 32 311 63 354 2.06e-22 96.3
MS.gene72126.t1 AT5G15940 31.579 304 172 12 32 311 62 353 2.43e-22 96.3
MS.gene72126.t1 AT5G15940 32.301 226 128 7 32 240 63 280 4.95e-17 80.5
MS.gene72126.t1 AT5G61830 27.148 291 156 11 32 285 39 310 3.95e-15 75.1
MS.gene72126.t1 AT5G61830 27.148 291 156 11 32 285 39 310 3.95e-15 75.1
MS.gene72126.t1 AT3G59710 27.143 280 158 8 31 283 20 280 2.12e-12 67.0
MS.gene72126.t1 AT3G59710 27.143 280 158 8 31 283 30 290 2.75e-12 66.6
MS.gene72126.t1 AT3G59710 27.143 280 158 8 31 283 49 309 3.38e-12 66.6

Find 60 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGGCCAGCTTTGTATCTT+TGG 0.197146 4.2:+6323129 None:intergenic
AAAGGTTTGTCAACAGAAAA+TGG 0.291840 4.2:+6324655 None:intergenic
CCACAGGTCAAGGGTATATC+TGG 0.344276 4.2:-6323196 MS.gene72126:CDS
TTAGCTCTTTCGCATCTGTT+AGG 0.393282 4.2:-6325409 MS.gene72126:CDS
ATGCAAAATATTTGCAAGCT+TGG 0.394712 4.2:+6324307 None:intergenic
ATATTAATTACCCAAGTGAT+TGG 0.395118 4.2:+6324799 None:intergenic
TGCATGCCAATGAGCTTTCA+AGG 0.395425 4.2:-6324289 MS.gene72126:CDS
TGTAAGTTTGTCTACAAAGT+AGG 0.397876 4.2:-6323089 None:intergenic
CAATAACTATCCTTCCTTCC+CGG 0.417908 4.2:+6324595 None:intergenic
CAAGTCACTCATGGAATTGA+TGG 0.419008 4.2:-6325696 MS.gene72126:CDS
AGCATCAAGTGGTCTTGGTC+TGG 0.420894 4.2:-6325567 MS.gene72126:CDS
TATCAAAGAAATGTTACTTA+AGG 0.421283 4.2:-6325471 MS.gene72126:CDS
TCAGGAGCATCAAGTGGTCT+TGG 0.423419 4.2:-6325572 MS.gene72126:intron
GTATCTTTGGCTAAAGAAGT+TGG 0.424566 4.2:+6323142 None:intergenic
CGGCACCATGGCTATTTCAA+TGG 0.427179 4.2:-6323877 MS.gene72126:intron
CAAGCGCCTTGAAAGCTCAT+TGG 0.429868 4.2:+6324283 None:intergenic
TATCTTTGGCTAAAGAAGTT+GGG 0.430163 4.2:+6323143 None:intergenic
AAACTGTAAGGGAATGCAAC+CGG 0.434447 4.2:-6324614 MS.gene72126:CDS
TGAGCTTTCAAGGCGCTTGA+AGG 0.461379 4.2:-6324279 MS.gene72126:intron
ATTGGTTGGAAAGGACCATC+TGG 0.466925 4.2:-6325744 MS.gene72126:CDS
TACATGTTGTTATGGCAGTT+AGG 0.474096 4.2:-6325514 MS.gene72126:CDS
ATGTGGTTTATTGGTTGGAA+AGG 0.477378 4.2:-6325753 MS.gene72126:CDS
TAACAATTGTACCAGGATGA+AGG 0.477833 4.2:+6323911 None:intergenic
AAAACATCAATCTTAGCAGC+TGG 0.481983 4.2:+6325443 None:intergenic
TCCTTTGTTTGACAGGAAGA+AGG 0.489471 4.2:-6323955 MS.gene72126:intron
GCGTGGTGTACATGTTGTTA+TGG 0.496049 4.2:-6325522 MS.gene72126:CDS
AATTCCATGAGTGACTTGTT+CGG 0.496592 4.2:+6325701 None:intergenic
GCAGTTTGCAACCAATCACT+TGG 0.498350 4.2:-6324810 MS.gene72126:CDS
TTGTCTTGAGAAAGCATGAA+TGG 0.505638 4.2:+6324842 None:intergenic
TGCATTGCATCCACAGGTCA+AGG 0.524363 4.2:-6323206 MS.gene72126:CDS
TGCAGCCATTGCTAATATGG+TGG 0.530297 4.2:-6323407 MS.gene72126:intron
GCAGCCATTGCTAATATGGT+GGG 0.536064 4.2:-6323406 MS.gene72126:intron
TCTCTCACTGCAATCATCAC+TGG 0.540397 4.2:-6325669 MS.gene72126:intron
AACTGTAAGGGAATGCAACC+GGG 0.543989 4.2:-6324613 MS.gene72126:CDS
TATATCTGGTGAATACTTTG+CGG 0.544760 4.2:-6323182 MS.gene72126:CDS
ACTGTTAACTCCCTTCATCC+TGG 0.557521 4.2:-6323922 MS.gene72126:CDS
GGGAATGCAACCGGGAAGGA+AGG 0.558048 4.2:-6324605 MS.gene72126:CDS
TTTAGCCAAAGATACAAAGC+TGG 0.558369 4.2:-6323134 MS.gene72126:CDS
TTACCTTGCATTGCATCCAC+AGG 0.567550 4.2:-6323212 MS.gene72126:CDS
CAGTTTGCAACCAATCACTT+GGG 0.575672 4.2:-6324809 MS.gene72126:intron
AACAATTGTACCAGGATGAA+GGG 0.577686 4.2:+6323912 None:intergenic
ACTGCCGAACAAGTCACTCA+TGG 0.598804 4.2:-6325705 MS.gene72126:CDS
AGTGATGATTGCAGTGAGAG+AGG 0.602845 4.2:+6325671 None:intergenic
ATATTTGTAACAATTGTACC+AGG 0.620073 4.2:+6323904 None:intergenic
TGTTACATTAGAATATTGAG+TGG 0.620095 4.2:+6325353 None:intergenic
ACAAATATTCTGCGGCACCA+TGG 0.621330 4.2:-6323889 MS.gene72126:CDS
GATAGTTATTGTATCATCAG+AGG 0.626892 4.2:-6324583 MS.gene72126:CDS
CTTGCCCACCATATTAGCAA+TGG 0.631089 4.2:+6323402 None:intergenic
CCAGATATACCCTTGACCTG+TGG 0.633382 4.2:+6323196 None:intergenic
GCATTGCATCCACAGGTCAA+GGG 0.636460 4.2:-6323205 MS.gene72126:CDS
ACTAACCATTGAAATAGCCA+TGG 0.642396 4.2:+6323872 None:intergenic
CAATTGTTACAAATATTCTG+CGG 0.643492 4.2:-6323897 MS.gene72126:CDS
AAGTAACAATGCAGGGACGA+TGG 0.645747 4.2:-6324870 MS.gene72126:CDS
GTCTTGGTCTGGAAACAACA+AGG 0.648423 4.2:-6325556 MS.gene72126:CDS
GATGAAGCTGAGAATCCAGA+TGG 0.651050 4.2:+6325729 None:intergenic
ACAAGGATTCTTGCTATGCG+TGG 0.652761 4.2:-6325539 MS.gene72126:CDS
GTAAGGGAATGCAACCGGGA+AGG 0.654933 4.2:-6324609 MS.gene72126:CDS
ATACTTTGCGGATAGCAACA+AGG 0.668282 4.2:-6323170 MS.gene72126:CDS
TGACCTGTGGATGCAATGCA+AGG 0.671162 4.2:+6323209 None:intergenic
AATATTGAGTGGAAGACCAG+AGG 0.674080 4.2:+6325364 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAGATAAATAAATAGT+TGG - chr4.2:6325221-6325240 MS.gene72126:intron 10.0%
!! TTATTTATCTTAATAACTAA+AGG + chr4.2:6325216-6325235 None:intergenic 10.0%
!!! AAAAAAAAACTTAATTCTTT+TGG + chr4.2:6325149-6325168 None:intergenic 10.0%
!!! ATATTTCCAAATTTTACAAT+AGG - chr4.2:6325289-6325308 MS.gene72126:intron 15.0%
!!! TTAATGATTTGATTATTTTC+AGG - chr4.2:6323253-6323272 MS.gene72126:intron 15.0%
!! AAATTATGTACAACAATAAC+CGG + chr4.2:6325376-6325395 None:intergenic 20.0%
!! AAGATATATGTTATAATGTG+AGG + chr4.2:6324076-6324095 None:intergenic 20.0%
!! AATAACTAAAGGATAACTTT+GGG + chr4.2:6325205-6325224 None:intergenic 20.0%
!! ATAATATGAATCCATATGTA+TGG + chr4.2:6323524-6323543 None:intergenic 20.0%
!! ATGTACAAAACAAAAATTGA+AGG - chr4.2:6324141-6324160 MS.gene72126:intron 20.0%
!! TAAACTCGATATTAATAAGT+TGG + chr4.2:6324729-6324748 None:intergenic 20.0%
!! TAAGATCCTATTGTAAAATT+TGG + chr4.2:6325298-6325317 None:intergenic 20.0%
!! TAATAACTAAAGGATAACTT+TGG + chr4.2:6325206-6325225 None:intergenic 20.0%
!!! AAAACTTAATTCTTTTGGTT+TGG + chr4.2:6325144-6325163 None:intergenic 20.0%
!!! AATATTTGATAGTATTTTGC+AGG - chr4.2:6324165-6324184 MS.gene72126:intron 20.0%
!!! GATCATTTTGTAATATTGAT+AGG - chr4.2:6323800-6323819 MS.gene72126:intron 20.0%
!!! TATCAAAGAAATGTTACTTA+AGG - chr4.2:6323372-6323391 MS.gene72126:intron 20.0%
!!! TTTTCATAGTCTCTAATAAA+AGG + chr4.2:6324209-6324228 None:intergenic 20.0%
! AAATTGGTACTTGAAATTGT+CGG - chr4.2:6323568-6323587 MS.gene72126:intron 25.0%
! AATCAGAGTAATTATCTAGT+TGG - chr4.2:6324334-6324353 MS.gene72126:CDS 25.0%
! AATCTGCAATCTATACATAA+AGG + chr4.2:6323916-6323935 None:intergenic 25.0%
! ACAATTTCTAACCATACATA+TGG - chr4.2:6323510-6323529 MS.gene72126:intron 25.0%
! AGACTATGAAAAAAACTGTA+AGG - chr4.2:6324217-6324236 MS.gene72126:intron 25.0%
! AGTGGGAAATTATAAAATTC+TGG + chr4.2:6324466-6324485 None:intergenic 25.0%
! ATATTAATTACCCAAGTGAT+TGG + chr4.2:6324047-6324066 None:intergenic 25.0%
! ATATTTGTAACAATTGTACC+AGG + chr4.2:6324942-6324961 None:intergenic 25.0%
! ATGAACAAAAATCCAAAACA+AGG + chr4.2:6325401-6325420 None:intergenic 25.0%
! CAATTCAATTAGTAAAAAGC+TGG + chr4.2:6325574-6325593 None:intergenic 25.0%
! CAATTGTTACAAATATTCTG+CGG - chr4.2:6324946-6324965 MS.gene72126:intron 25.0%
! GACTATGAAAAAAACTGTAA+GGG - chr4.2:6324218-6324237 MS.gene72126:intron 25.0%
! GTGGGAAATTATAAAATTCT+GGG + chr4.2:6324465-6324484 None:intergenic 25.0%
! TAATCAGCAATAGTTGAAAA+TGG + chr4.2:6325004-6325023 None:intergenic 25.0%
! TATCTGTAAAGAATCAAACA+GGG + chr4.2:6324490-6324509 None:intergenic 25.0%
! TCGTAAGATTCAATCTTATT+TGG - chr4.2:6324619-6324638 MS.gene72126:CDS 25.0%
! TGTTACATTAGAATATTGAG+TGG + chr4.2:6323493-6323512 None:intergenic 25.0%
! TTATGTATAGATTGCAGATT+TGG - chr4.2:6323916-6323935 MS.gene72126:CDS 25.0%
! TTTCGACTAAATACCTAAAT+TGG - chr4.2:6323552-6323571 MS.gene72126:intron 25.0%
!! ACAATTTCAAGTACCAATTT+AGG + chr4.2:6323568-6323587 None:intergenic 25.0%
!! ACAGATTTTCATATAGTGAA+GGG - chr4.2:6324286-6324305 MS.gene72126:CDS 25.0%
!! ACTTAATTCCCATAGTTTTT+GGG + chr4.2:6325735-6325754 None:intergenic 25.0%
!! GATTAAGTATCCTTTTTGTT+TGG - chr4.2:6325019-6325038 MS.gene72126:intron 25.0%
!! GCTTTTTACTAATTGAATTG+TGG - chr4.2:6325574-6325593 MS.gene72126:intron 25.0%
!! TTTTACTAATTGAATTGTGG+TGG - chr4.2:6325577-6325596 MS.gene72126:intron 25.0%
!!! TACATAATTTCTCCTTGTTT+TGG - chr4.2:6325386-6325405 MS.gene72126:CDS 25.0%
AAAGGTTTGTCAACAGAAAA+TGG + chr4.2:6324191-6324210 None:intergenic 30.0%
AACAATTTCAAGAACCAAGT+TGG - chr4.2:6323835-6323854 MS.gene72126:intron 30.0%
AATAAAATCTGTGCATCCAT+TGG - chr4.2:6323656-6323675 MS.gene72126:intron 30.0%
AATTGGTACTTGAAATTGTC+GGG - chr4.2:6323569-6323588 MS.gene72126:intron 30.0%
ATGCAAAATATTTGCAAGCT+TGG + chr4.2:6324539-6324558 None:intergenic 30.0%
GAGTGAATAAATAACCAACT+TGG + chr4.2:6323852-6323871 None:intergenic 30.0%
GATAGTTATTGTATCATCAG+AGG - chr4.2:6324260-6324279 MS.gene72126:intron 30.0%
GTATCTGTAAAGAATCAAAC+AGG + chr4.2:6324491-6324510 None:intergenic 30.0%
TAAAGATTTCGATAACGCAA+GGG + chr4.2:6323610-6323629 None:intergenic 30.0%
TAAATAGACTGCTTCAGTAA+TGG - chr4.2:6324807-6324826 MS.gene72126:intron 30.0%
TAGGATCTTACAAATAGAGA+CGG - chr4.2:6325308-6325327 MS.gene72126:intron 30.0%
TATATCTGGTGAATACTTTG+CGG - chr4.2:6325661-6325680 MS.gene72126:intron 30.0%
TATCTTTGGCTAAAGAAGTT+GGG + chr4.2:6325703-6325722 None:intergenic 30.0%
TCAAACAAAGGAATATGCAA+GGG + chr4.2:6324880-6324899 None:intergenic 30.0%
TCAATGGAGTCTTACAAATA+GGG + chr4.2:6325121-6325140 None:intergenic 30.0%
TGAAGCAGTCTATTTACAAT+GGG + chr4.2:6324803-6324822 None:intergenic 30.0%
TTAAAGATTTCGATAACGCA+AGG + chr4.2:6323611-6323630 None:intergenic 30.0%
! AACTTGGTTCTTGAAATTGT+TGG + chr4.2:6323836-6323855 None:intergenic 30.0%
! ATACCTGTTGGACATTTTTA+AGG + chr4.2:6325472-6325491 None:intergenic 30.0%
! CACAGATTTTCATATAGTGA+AGG - chr4.2:6324285-6324304 MS.gene72126:CDS 30.0%
! CACTTAATTCCCATAGTTTT+TGG + chr4.2:6325736-6325755 None:intergenic 30.0%
! TGTGTTTAAAGCAGTCTTAT+GGG - chr4.2:6324699-6324718 MS.gene72126:intron 30.0%
! TTCATCTAACTCAGTTTTTG+AGG + chr4.2:6323233-6323252 None:intergenic 30.0%
!! GTATCCTTTTTGTTTGGAAT+AGG - chr4.2:6325025-6325044 MS.gene72126:intron 30.0%
!! TACAGCAGTTATTTTGCTTA+TGG - chr4.2:6324508-6324527 MS.gene72126:intron 30.0%
!! TGATCTCTAGAATTGCTAAA+TGG + chr4.2:6323785-6323804 None:intergenic 30.0%
!!! AATTCTTTTGGTTTGGTCAA+TGG + chr4.2:6325137-6325156 None:intergenic 30.0%
!!! GTTGGTCTTTTAATTCTCTT+TGG - chr4.2:6325239-6325258 MS.gene72126:intron 30.0%
!!! TCTTTTCAAGTAACAATGCA+GGG - chr4.2:6323966-6323985 MS.gene72126:intron 30.0%
!!! TTCTTTTCAAGTAACAATGC+AGG - chr4.2:6323965-6323984 MS.gene72126:intron 30.0%
AAAACATCAATCTTAGCAGC+TGG + chr4.2:6323403-6323422 None:intergenic 35.0%
AAAGAATCAAACAGGGAAGT+GGG + chr4.2:6324483-6324502 None:intergenic 35.0%
AACAATTGTACCAGGATGAA+GGG + chr4.2:6324934-6324953 None:intergenic 35.0%
AATAGGTCTGATGTCCTATT+CGG - chr4.2:6325042-6325061 MS.gene72126:intron 35.0%
ACTAAAAAATTGTGAGCCTG+TGG + chr4.2:6325499-6325518 None:intergenic 35.0%
ACTAACCATTGAAATAGCCA+TGG + chr4.2:6324974-6324993 None:intergenic 35.0%
CAGACCTATTCCAAACAAAA+AGG + chr4.2:6325032-6325051 None:intergenic 35.0%
CGAGTTTATTGAAATCCAAG+CGG - chr4.2:6324741-6324760 MS.gene72126:intron 35.0%
CTGAAGCAGTCTATTTACAA+TGG + chr4.2:6324804-6324823 None:intergenic 35.0%
CTTCCTTAAAAATGTCCAAC+AGG - chr4.2:6325466-6325485 MS.gene72126:CDS 35.0%
GCAGTTAGGAATGTGAAAAA+TGG - chr4.2:6323343-6323362 MS.gene72126:intron 35.0%
GTATCTTTGGCTAAAGAAGT+TGG + chr4.2:6325704-6325723 None:intergenic 35.0%
GTCAAACAAAGGAATATGCA+AGG + chr4.2:6324881-6324900 None:intergenic 35.0%
GTCAATGGAGTCTTACAAAT+AGG + chr4.2:6325122-6325141 None:intergenic 35.0%
TAAAGAATCAAACAGGGAAG+TGG + chr4.2:6324484-6324503 None:intergenic 35.0%
TAACAATTGTACCAGGATGA+AGG + chr4.2:6324935-6324954 None:intergenic 35.0%
TACATGTTGTTATGGCAGTT+AGG - chr4.2:6323329-6323348 MS.gene72126:intron 35.0%
TATGTACGAGTCATACTAAC+CGG - chr4.2:6325354-6325373 MS.gene72126:intron 35.0%
TCTTTCCTTTATCTGCTACA+AGG - chr4.2:6324378-6324397 MS.gene72126:intron 35.0%
TGACTAGACGATGAACTTAT+TGG - chr4.2:6324837-6324856 MS.gene72126:CDS 35.0%
TGCATCCATTGGAATTTGTA+GGG - chr4.2:6323667-6323686 MS.gene72126:intron 35.0%
TGTCAGAAGATCTAGATAGA+TGG - chr4.2:6323746-6323765 MS.gene72126:intron 35.0%
TTACCATCACGTTACTCTAA+CGG + chr4.2:6324650-6324669 None:intergenic 35.0%
TTTAGCCAAAGATACAAAGC+TGG - chr4.2:6325709-6325728 MS.gene72126:CDS 35.0%
! ATGTGGTTTATTGGTTGGAA+AGG - chr4.2:6323090-6323109 MS.gene72126:CDS 35.0%
! CTGTGTTTAAAGCAGTCTTA+TGG - chr4.2:6324698-6324717 MS.gene72126:intron 35.0%
! TAAGACTGCTTTAAACACAG+TGG + chr4.2:6324698-6324717 None:intergenic 35.0%
! TGCATATTCCTTTGTTTGAC+AGG - chr4.2:6324881-6324900 MS.gene72126:intron 35.0%
! TTGTCTTGAGAAAGCATGAA+TGG + chr4.2:6324004-6324023 None:intergenic 35.0%
! TTTTGCAGCCATTGCTAATA+TGG - chr4.2:6325433-6325452 MS.gene72126:CDS 35.0%
!! AATTCCATGAGTGACTTGTT+CGG + chr4.2:6323145-6323164 None:intergenic 35.0%
!!! TTATTTTCAGGAGCATCAAG+TGG - chr4.2:6323265-6323284 MS.gene72126:intron 35.0%
AAACTGTAAGGGAATGCAAC+CGG - chr4.2:6324229-6324248 MS.gene72126:intron 40.0%
AAAGCTGGCCCAAAAACTAT+GGG - chr4.2:6325724-6325743 MS.gene72126:CDS 40.0%
AATATTGAGTGGAAGACCAG+AGG + chr4.2:6323482-6323501 None:intergenic 40.0%
AGCAGTCTATTTACAATGGG+AGG + chr4.2:6324800-6324819 None:intergenic 40.0%
ATACTTTGCGGATAGCAACA+AGG - chr4.2:6325673-6325692 MS.gene72126:CDS 40.0%
CAAGTCACTCATGGAATTGA+TGG - chr4.2:6323147-6323166 MS.gene72126:CDS 40.0%
CAATAACTATCCTTCCTTCC+CGG + chr4.2:6324251-6324270 None:intergenic 40.0%
CAGTTTGCAACCAATCACTT+GGG - chr4.2:6324034-6324053 MS.gene72126:intron 40.0%
CTAGTCCCTACAAATTCCAA+TGG + chr4.2:6323675-6323694 None:intergenic 40.0%
GTGCATCCATTGGAATTTGT+AGG - chr4.2:6323666-6323685 MS.gene72126:intron 40.0%
TCCTTCTTCCTGTCAAACAA+AGG + chr4.2:6324892-6324911 None:intergenic 40.0%
! ATGAGCCTTGTAGCAGATAA+AGG + chr4.2:6324386-6324405 None:intergenic 40.0%
! TCCTTTGTTTGACAGGAAGA+AGG - chr4.2:6324888-6324907 MS.gene72126:intron 40.0%
! TTAGCTCTTTCGCATCTGTT+AGG - chr4.2:6323434-6323453 MS.gene72126:intron 40.0%
!!! GCAGCAGATTTTAATTCCTC+TGG - chr4.2:6323463-6323482 MS.gene72126:intron 40.0%
AACTGTAAGGGAATGCAACC+GGG - chr4.2:6324230-6324249 MS.gene72126:intron 45.0%
AAGTAACAATGCAGGGACGA+TGG - chr4.2:6323973-6323992 MS.gene72126:intron 45.0%
ACAAATATTCTGCGGCACCA+TGG - chr4.2:6324954-6324973 MS.gene72126:intron 45.0%
ACGTGATGGTAACTCGTTGA+CGG - chr4.2:6324658-6324677 MS.gene72126:CDS 45.0%
ACTGTTAACTCCCTTCATCC+TGG - chr4.2:6324921-6324940 MS.gene72126:intron 45.0%
AGTGATGATTGCAGTGAGAG+AGG + chr4.2:6323175-6323194 None:intergenic 45.0%
ATGACCAACCAGAACCGAAT+AGG + chr4.2:6325059-6325078 None:intergenic 45.0%
ATTGGTTGGAAAGGACCATC+TGG - chr4.2:6323099-6323118 MS.gene72126:CDS 45.0%
CAAAGCTGGCCCAAAAACTA+TGG - chr4.2:6325723-6325742 MS.gene72126:CDS 45.0%
CAACCGTTAGAGTAACGTGA+TGG - chr4.2:6324644-6324663 MS.gene72126:CDS 45.0%
CTTGCCCACCATATTAGCAA+TGG + chr4.2:6325444-6325463 None:intergenic 45.0%
GATGAAGCTGAGAATCCAGA+TGG + chr4.2:6323117-6323136 None:intergenic 45.0%
GCAGCCATTGCTAATATGGT+GGG - chr4.2:6325437-6325456 MS.gene72126:CDS 45.0%
GCAGTTTGCAACCAATCACT+TGG - chr4.2:6324033-6324052 MS.gene72126:intron 45.0%
GCGTGGTGTACATGTTGTTA+TGG - chr4.2:6323321-6323340 MS.gene72126:intron 45.0%
GTCTTGGTCTGGAAACAACA+AGG - chr4.2:6323287-6323306 MS.gene72126:intron 45.0%
TCTCTCACTGCAATCATCAC+TGG - chr4.2:6323174-6323193 MS.gene72126:CDS 45.0%
TCTGATGTCCTATTCGGTTC+TGG - chr4.2:6325048-6325067 MS.gene72126:intron 45.0%
TGCAGCCATTGCTAATATGG+TGG - chr4.2:6325436-6325455 MS.gene72126:CDS 45.0%
TGCATGCCAATGAGCTTTCA+AGG - chr4.2:6324554-6324573 MS.gene72126:CDS 45.0%
TTACCTTGCATTGCATCCAC+AGG - chr4.2:6325631-6325650 MS.gene72126:intron 45.0%
! ACAAGGATTCTTGCTATGCG+TGG - chr4.2:6323304-6323323 MS.gene72126:intron 45.0%
! ATGTCCTATTCGGTTCTGGT+TGG - chr4.2:6325052-6325071 MS.gene72126:intron 45.0%
! GTGGTGGCACTTCTTTTGTA+GGG - chr4.2:6325593-6325612 MS.gene72126:intron 45.0%
! TCCAACAGGTATTTTGCCAC+AGG - chr4.2:6325480-6325499 MS.gene72126:CDS 45.0%
! TCGGTTCTGGTTGGTCATAT+TGG - chr4.2:6325061-6325080 MS.gene72126:intron 45.0%
! TGTGGTGGCACTTCTTTTGT+AGG - chr4.2:6325592-6325611 MS.gene72126:intron 45.0%
! TTGGGCCAGCTTTGTATCTT+TGG + chr4.2:6325717-6325736 None:intergenic 45.0%
!! ACGAGCTATTTTCTTCCGCT+TGG + chr4.2:6324759-6324778 None:intergenic 45.0%
ACTGCCGAACAAGTCACTCA+TGG - chr4.2:6323138-6323157 MS.gene72126:CDS 50.0%
CAAGCGCCTTGAAAGCTCAT+TGG + chr4.2:6324563-6324582 None:intergenic 50.0%
CCAGATATACCCTTGACCTG+TGG + chr4.2:6325650-6325669 None:intergenic 50.0%
GCATTGCATCCACAGGTCAA+GGG - chr4.2:6325638-6325657 MS.gene72126:intron 50.0%
GCCTGTGGCAAAATACCTGT+TGG + chr4.2:6325484-6325503 None:intergenic 50.0%
TGACCTGTGGATGCAATGCA+AGG + chr4.2:6325637-6325656 None:intergenic 50.0%
TGCATTGCATCCACAGGTCA+AGG - chr4.2:6325637-6325656 MS.gene72126:intron 50.0%
! AGCATCAAGTGGTCTTGGTC+TGG - chr4.2:6323276-6323295 MS.gene72126:intron 50.0%
! CCACAGGTCAAGGGTATATC+TGG - chr4.2:6325647-6325666 MS.gene72126:intron 50.0%
! CGGCACCATGGCTATTTCAA+TGG - chr4.2:6324966-6324985 MS.gene72126:intron 50.0%
! TGAGCTTTCAAGGCGCTTGA+AGG - chr4.2:6324564-6324583 MS.gene72126:CDS 50.0%
!! TCAGGAGCATCAAGTGGTCT+TGG - chr4.2:6323271-6323290 MS.gene72126:intron 50.0%
GTAAGGGAATGCAACCGGGA+AGG - chr4.2:6324234-6324253 MS.gene72126:intron 55.0%
GGGAATGCAACCGGGAAGGA+AGG - chr4.2:6324238-6324257 MS.gene72126:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 6323090 6325775 6323090 ID=MS.gene72126
chr4.2 mRNA 6323090 6325775 6323090 ID=MS.gene72126.t1;Parent=MS.gene72126
chr4.2 exon 6325670 6325775 6325670 ID=MS.gene72126.t1.exon1;Parent=MS.gene72126.t1
chr4.2 CDS 6325670 6325775 6325670 ID=cds.MS.gene72126.t1;Parent=MS.gene72126.t1
chr4.2 exon 6325359 6325590 6325359 ID=MS.gene72126.t1.exon2;Parent=MS.gene72126.t1
chr4.2 CDS 6325359 6325590 6325359 ID=cds.MS.gene72126.t1;Parent=MS.gene72126.t1
chr4.2 exon 6324810 6324889 6324810 ID=MS.gene72126.t1.exon3;Parent=MS.gene72126.t1
chr4.2 CDS 6324810 6324889 6324810 ID=cds.MS.gene72126.t1;Parent=MS.gene72126.t1
chr4.2 exon 6324525 6324678 6324525 ID=MS.gene72126.t1.exon4;Parent=MS.gene72126.t1
chr4.2 CDS 6324525 6324678 6324525 ID=cds.MS.gene72126.t1;Parent=MS.gene72126.t1
chr4.2 exon 6324280 6324358 6324280 ID=MS.gene72126.t1.exon5;Parent=MS.gene72126.t1
chr4.2 CDS 6324280 6324358 6324280 ID=cds.MS.gene72126.t1;Parent=MS.gene72126.t1
chr4.2 exon 6323878 6323962 6323878 ID=MS.gene72126.t1.exon6;Parent=MS.gene72126.t1
chr4.2 CDS 6323878 6323962 6323878 ID=cds.MS.gene72126.t1;Parent=MS.gene72126.t1
chr4.2 exon 6323378 6323424 6323378 ID=MS.gene72126.t1.exon7;Parent=MS.gene72126.t1
chr4.2 CDS 6323378 6323424 6323378 ID=cds.MS.gene72126.t1;Parent=MS.gene72126.t1
chr4.2 exon 6323090 6323251 6323090 ID=MS.gene72126.t1.exon8;Parent=MS.gene72126.t1
chr4.2 CDS 6323090 6323251 6323090 ID=cds.MS.gene72126.t1;Parent=MS.gene72126.t1
Gene Sequence

>MS.gene72126

ATGTGGTTTATTGGTTGGAAAGGACCATCTGGATTCTCAGCTTCATCAACTGCCGAACAAGTCACTCATGGAATTGATGGAACCTCTCTCACTGCAATCATCACTGGTTTTTTCTTGTTCAATTTATTGTGTTTTTTATTCCTCAAAAACTGAGTTAGATGAATTAATGATTTGATTATTTTCAGGAGCATCAAGTGGTCTTGGTCTGGAAACAACAAGGATTCTTGCTATGCGTGGTGTACATGTTGTTATGGCAGTTAGGAATGTGAAAAATGGTATAGATATCAAAGAAATGTTACTTAAGGAAATTCCAGCTGCTAAGATTGATGTTTTTGAGTTAGATCTTAGCTCTTTCGCATCTGTTAGGAAATTCGCAGCAGATTTTAATTCCTCTGGTCTTCCACTCAATATTCTAATGTAACAATTTCTAACCATACATATGGATTCATATTATTATTTAAATTTCGACTAAATACCTAAATTGGTACTTGAAATTGTCGGGTTGTATCAATCTAGTCCCTTGCGTTATCGAAATCTTTAAATGATCTCTGAAATTGCAAAGTTTTAATAAAATCTGTGCATCCATTGGAATTTGTAGGGACTAGAATGACATTAAGTGTGTAATATCAGAGATGAATTTGAAACTAAGTGACTAATGTCAGAAGATCTAGATAGATGGATGAAGTTGATTACCATTTAGCAATTCTAGAGATCATTTTGTAATATTGATAGGTTGATACGATCCAACAATTTCAAGAACCAAGTTGGTTATTTATTCACTCTTTAAATTTTAAATTGAGCAGCAAAAAAATACTACTATATACCTTTATGTATAGATTGCAGATTTGGAAGTTGAATCAAATAATATAGTCTTTTTCTTTTCAAGTAACAATGCAGGGACGATGGCTACTCCATTCATGCTTTCTCAAGACAATATTGAATTGCAGTTTGCAACCAATCACTTGGGTAATTAATATATGTTTCCTCACATTATAACATATATCTTTCTAAATGATACAACTGATAGAAAGTTGTTATTTACTCTCTGATAATGTACAAAACAAAAATTGAAGGTAATATTTGATAGTATTTTGCAGGCCATTTTCTGTTGACAAACCTTTTATTAGAGACTATGAAAAAAACTGTAAGGGAATGCAACCGGGAAGGAAGGATAGTTATTGTATCATCAGAGGCTCACAGATTTTCATATAGTGAAGGGATTCGTTTCGATAAGATCAACAACGAATCAGAGTAATTATCTAGTTGGAATGTTTAATTTTTTAATTATTCTTTCCTTTATCTGCTACAAGGCTCATTCTATTAAGTTTTTTATTTTATTAAATGTTAATCATCATGTTCAGTGTTCTAAGTTCCCAGAATTTTATAATTTCCCACTTCCCTGTTTGATTCTTTACAGATACAGCAGTTATTTTGCTTATGGACAATCCAAGCTTGCAAATATTTTGCATGCCAATGAGCTTTCAAGGCGCTTGAAGGTATTATAGTCGCACAAAACTGTTTATGCAATTTCGTAAGATTCAATCTTATTTGGTGCAACCGTTAGAGTAACGTGATGGTAACTCGTTGACGGTTCGAGAAAATTTACCACTGTGTTTAAAGCAGTCTTATGGGACAACCAACTTATTAATATCGAGTTTATTGAAATCCAAGCGGAAGAAAATAGCTCGTTTGTCTACTGCATAGATTCCTCCCATTGTAAATAGACTGCTTCAGTAATGGATTTTCTTGACTAGACGATGAACTTATTGGTTGAGTTTGATGTTGAACCCTTGCATATTCCTTTGTTTGACAGGAAGAAGGAGTGCAAATAACTGTTAACTCCCTTCATCCTGGTACAATTGTTACAAATATTCTGCGGCACCATGGCTATTTCAATGGTTAGTATATATACCATTTTCAACTATTGCTGATTAAGTATCCTTTTTGTTTGGAATAGGTCTGATGTCCTATTCGGTTCTGGTTGGTCATATTGGTTAACACTGATGTAATATATAATATTAGATATATCCCTATTTGTAAGACTCCATTGACCAAACCAAAAGAATTAAGTTTTTTTTTTGTGTTAAATTTGTCAATAACGTATACTCTATTCCCAAAGTTATCCTTTAGTTATTAAGATAAATAAATAGTTGGTCTTTTAATTCTCTTTGGTCTTTTAATTCTCTTCATTTAATCAAGATATTTCCAAATTTTACAATAGGATCTTACAAATAGAGACGGATGTAGTAATATTGTTGTCATGCTATGTACGAGTCATACTAACCGGTTATTGTTGTACATAATTTCTCCTTGTTTTGGATTTTTGTTCATGTGTCGCTCGATTTTTGCAGCCATTGCTAATATGGTGGGCAAGTACTTCCTTAAAAATGTCCAACAGGTATTTTGCCACAGGCTCACAATTTTTTAGTCAACATGCATGCAAGTCATCTGCAGCTAGTAGACTAAAGACTAATTGTTAGACCAGCTTTTTACTAATTGAATTGTGGTGGCACTTCTTTTGTAGGGAGCTGCAACTCAATGTTACCTTGCATTGCATCCACAGGTCAAGGGTATATCTGGTGAATACTTTGCGGATAGCAACAAGGCAAGCCCAACTTCTTTAGCCAAAGATACAAAGCTGGCCCAAAAACTATGGGAATTAAGTGTAAGTTTGTCTACAAAGTAG
Protein sequence

>MS.gene72126.t1

MWFIGWKGPSGFSASSTAEQVTHGIDGTSLTAIITGASSGLGLETTRILAMRGVHVVMAVRNVKNGIDIKEMLLKEIPAAKIDVFELDLSSFASVRKFAADFNSSGLPLNILINNAGTMATPFMLSQDNIELQFATNHLGHFLLTNLLLETMKKTVRECNREGRIVIVSSEAHRFSYSEGIRFDKINNESEYSSYFAYGQSKLANILHANELSRRLKEEGVQITVNSLHPGTIVTNILRHHGYFNAIANMVGKYFLKNVQQGAATQCYLALHPQVKGISGEYFADSNKASPTSLAKDTKLAQKLWELSVSLSTK