Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74220.t1 | XP_003592798.1 | 85.7 | 175 | 21 | 2 | 1 | 175 | 1 | 171 | 8.20E-63 | 250 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74220.t1 | Q6YUA8 | 71.9 | 121 | 27 | 1 | 62 | 175 | 67 | 187 | 9.3e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74220.t1 | G7IF33 | 85.7 | 175 | 21 | 2 | 1 | 175 | 1 | 171 | 5.9e-63 | 250.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene74220 | 0.80224 | 6.16E-49 | -1.69E-46 |
| MS.gene049170 | MS.gene74220 | 0.809152 | 2.18E-50 | -1.69E-46 |
| MS.gene050404 | MS.gene74220 | 0.808768 | 2.63E-50 | -1.69E-46 |
| MS.gene050529 | MS.gene74220 | 0.813233 | 2.84E-51 | -1.69E-46 |
| MS.gene050812 | MS.gene74220 | 0.854832 | 9.46E-62 | -1.69E-46 |
| MS.gene05081 | MS.gene74220 | 0.840202 | 1.00E-57 | -1.69E-46 |
| MS.gene051171 | MS.gene74220 | 0.835743 | 1.40E-56 | -1.69E-46 |
| MS.gene051242 | MS.gene74220 | 0.844637 | 6.68E-59 | -1.69E-46 |
| MS.gene051244 | MS.gene74220 | 0.856051 | 4.18E-62 | -1.69E-46 |
| MS.gene051617 | MS.gene74220 | 0.806128 | 9.56E-50 | -1.69E-46 |
| MS.gene051666 | MS.gene74220 | 0.805972 | 1.03E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene74220 | 0.820877 | 5.44E-53 | -1.69E-46 |
| MS.gene052134 | MS.gene74220 | 0.812512 | 4.08E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene74220 | 0.801748 | 7.78E-49 | -1.69E-46 |
| MS.gene052469 | MS.gene74220 | 0.844734 | 6.29E-59 | -1.69E-46 |
| MS.gene052870 | MS.gene74220 | 0.845621 | 3.62E-59 | -1.69E-46 |
| MS.gene052872 | MS.gene74220 | 0.844307 | 8.20E-59 | -1.69E-46 |
| MS.gene053103 | MS.gene74220 | 0.800673 | 1.29E-48 | -1.69E-46 |
| MS.gene053104 | MS.gene74220 | 0.804832 | 1.79E-49 | -1.69E-46 |
| MS.gene053107 | MS.gene74220 | 0.805652 | 1.20E-49 | -1.69E-46 |
| MS.gene053330 | MS.gene74220 | 0.852458 | 4.56E-61 | -1.69E-46 |
| MS.gene053971 | MS.gene74220 | 0.814159 | 1.78E-51 | -1.69E-46 |
| MS.gene054884 | MS.gene74220 | -0.836261 | 1.04E-56 | -1.69E-46 |
| MS.gene055206 | MS.gene74220 | 0.808395 | 3.16E-50 | -1.69E-46 |
| MS.gene055370 | MS.gene74220 | 0.865447 | 5.87E-65 | -1.69E-46 |
| MS.gene055539 | MS.gene74220 | 0.819451 | 1.15E-52 | -1.69E-46 |
| MS.gene05553 | MS.gene74220 | 0.812451 | 4.21E-51 | -1.69E-46 |
| MS.gene055605 | MS.gene74220 | 0.801219 | 9.98E-49 | -1.69E-46 |
| MS.gene056633 | MS.gene74220 | 0.883503 | 4.25E-71 | -1.69E-46 |
| MS.gene056664 | MS.gene74220 | 0.886925 | 2.24E-72 | -1.69E-46 |
| MS.gene056678 | MS.gene74220 | -0.837933 | 3.88E-57 | -1.69E-46 |
| MS.gene056796 | MS.gene74220 | 0.824328 | 8.57E-54 | -1.69E-46 |
| MS.gene056903 | MS.gene74220 | 0.825139 | 5.52E-54 | -1.69E-46 |
| MS.gene057032 | MS.gene74220 | 0.832733 | 7.99E-56 | -1.69E-46 |
| MS.gene057196 | MS.gene74220 | 0.831604 | 1.52E-55 | -1.69E-46 |
| MS.gene057332 | MS.gene74220 | 0.84225 | 2.90E-58 | -1.69E-46 |
| MS.gene057440 | MS.gene74220 | -0.801089 | 1.06E-48 | -1.69E-46 |
| MS.gene057658 | MS.gene74220 | 0.836036 | 1.18E-56 | -1.69E-46 |
| MS.gene058233 | MS.gene74220 | 0.832973 | 6.96E-56 | -1.69E-46 |
| MS.gene058276 | MS.gene74220 | 0.843472 | 1.37E-58 | -1.69E-46 |
| MS.gene058746 | MS.gene74220 | 0.876567 | 1.26E-68 | -1.69E-46 |
| MS.gene058838 | MS.gene74220 | 0.805441 | 1.33E-49 | -1.69E-46 |
| MS.gene059023 | MS.gene74220 | 0.810935 | 9.00E-51 | -1.69E-46 |
| MS.gene05914 | MS.gene74220 | 0.852825 | 3.58E-61 | -1.69E-46 |
| MS.gene05934 | MS.gene74220 | 0.804744 | 1.87E-49 | -1.69E-46 |
| MS.gene059512 | MS.gene74220 | 0.809744 | 1.63E-50 | -1.69E-46 |
| MS.gene060087 | MS.gene74220 | -0.801787 | 7.64E-49 | -1.69E-46 |
| MS.gene060407 | MS.gene74220 | 0.830284 | 3.20E-55 | -1.69E-46 |
| MS.gene06092 | MS.gene74220 | 0.818699 | 1.71E-52 | -1.69E-46 |
| MS.gene061150 | MS.gene74220 | 0.802132 | 6.49E-49 | -1.69E-46 |
| MS.gene061231 | MS.gene74220 | 0.854457 | 1.22E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74220.t1 | MTR_1g116170 | 89.143 | 175 | 15 | 2 | 1 | 175 | 1 | 171 | 5.14e-109 | 308 |
| MS.gene74220.t1 | MTR_1g116170 | 85.714 | 133 | 15 | 2 | 1 | 133 | 1 | 129 | 4.97e-77 | 226 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74220.t1 | AT2G34860 | 56.069 | 173 | 64 | 4 | 15 | 175 | 14 | 186 | 6.15e-59 | 182 |
| MS.gene74220.t1 | AT2G34860 | 56.069 | 173 | 64 | 4 | 15 | 175 | 14 | 186 | 6.15e-59 | 182 |
Find 41 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCTTATCAAGCAGTTTCT+TGG | 0.222839 | 1.2:+86258739 | None:intergenic |
| ACATACCTTCTTCTAAGAAT+TGG | 0.272269 | 1.2:+86258697 | None:intergenic |
| CTTGTCTGCCCTGTTTGTTT+AGG | 0.322236 | 1.2:-86258806 | MS.gene74220:CDS |
| ACTGAATGTCCTAATTGTTA+TGG | 0.323214 | 1.2:-86258839 | MS.gene74220:CDS |
| CGGAATGGATTGCAATTTGC+AGG | 0.323737 | 1.2:-86259419 | None:intergenic |
| CTGAGAAGACCTTTATTGTT+TGG | 0.336791 | 1.2:+86258773 | None:intergenic |
| TTTCCTCGACCATAACAATT+AGG | 0.345884 | 1.2:+86258830 | None:intergenic |
| TGCCCTGTTTGTTTAGGAAC+TGG | 0.352275 | 1.2:-86258800 | MS.gene74220:CDS |
| CTGCTTGATAAGATGTATAA+TGG | 0.353896 | 1.2:-86258731 | MS.gene74220:CDS |
| GAAAATGGAAAGCTCTTAAC+AGG | 0.369385 | 1.2:-86258889 | MS.gene74220:CDS |
| CATACCTTCTTCTAAGAATT+GGG | 0.381922 | 1.2:+86258698 | None:intergenic |
| TGTGTGGTGGTACAGGAAAA+TGG | 0.392406 | 1.2:-86258904 | MS.gene74220:CDS |
| TCAAGCAGTTTCTTGGCGTC+AGG | 0.394589 | 1.2:+86258746 | None:intergenic |
| ACTGGTGTGCCAAACAATAA+AGG | 0.405844 | 1.2:-86258782 | MS.gene74220:CDS |
| ACACCAGTTCCTAAACAAAC+AGG | 0.409014 | 1.2:+86258797 | None:intergenic |
| AGTTGGAAGAGGAGGAAGAG+TGG | 0.470888 | 1.2:+86259293 | None:intergenic |
| ACCGCTCTTGATAAACCTCC+AGG | 0.498198 | 1.2:-86259084 | MS.gene74220:CDS |
| CCACCACAATTTCTGCAACC+TGG | 0.498937 | 1.2:+86259066 | None:intergenic |
| GTTTGTTTGATAAAAGAAGT+TGG | 0.500841 | 1.2:+86259370 | None:intergenic |
| TCCACTCTTCCTCAAAATCA+AGG | 0.504399 | 1.2:-86259319 | MS.gene74220:CDS |
| CAAACAATAAAGGTCTTCTC+AGG | 0.512552 | 1.2:-86258772 | MS.gene74220:CDS |
| GGTGATATGTGTGGTGGTAC+AGG | 0.517437 | 1.2:-86258911 | MS.gene74220:CDS |
| TGCAGAAATTGTGGTGGAAG+TGG | 0.524731 | 1.2:-86259060 | MS.gene74220:CDS |
| TCTTCTAAGAATTGGGAAGT+AGG | 0.526104 | 1.2:+86258705 | None:intergenic |
| ACATACCTTGGAGTTGGAAG+AGG | 0.537745 | 1.2:+86259282 | None:intergenic |
| TGATAAAAGAAGTTGGAGGA+AGG | 0.545348 | 1.2:+86259377 | None:intergenic |
| TGTCCTAATTGTTATGGTCG+AGG | 0.554383 | 1.2:-86258833 | MS.gene74220:CDS |
| CCTCCAGGTTGCAGAAATTG+TGG | 0.558014 | 1.2:-86259069 | MS.gene74220:CDS |
| ATACATACATACCTTGGAGT+TGG | 0.560487 | 1.2:+86259276 | None:intergenic |
| ACATACATACATACATACCT+TGG | 0.567889 | 1.2:+86259270 | None:intergenic |
| TACCTTGGAGTTGGAAGAGG+AGG | 0.582188 | 1.2:+86259285 | None:intergenic |
| TTCCTCCTCTTCCAACTCCA+AGG | 0.584619 | 1.2:-86259287 | MS.gene74220:CDS |
| ACCTGGAGGTTTATCAAGAG+CGG | 0.598366 | 1.2:+86259083 | None:intergenic |
| CATTTAGGTGATATGTGTGG+TGG | 0.616105 | 1.2:-86258917 | MS.gene74220:intron |
| ACAATAAAGGTCTTCTCAGG+AGG | 0.622506 | 1.2:-86258769 | MS.gene74220:CDS |
| CCACAATTTCTGCAACCTGG+AGG | 0.630868 | 1.2:+86259069 | None:intergenic |
| CCAGGTTGCAGAAATTGTGG+TGG | 0.631837 | 1.2:-86259066 | MS.gene74220:CDS |
| TTTCATTTAGGTGATATGTG+TGG | 0.646439 | 1.2:-86258920 | MS.gene74220:intron |
| CACCAGTTCCTAAACAAACA+GGG | 0.654127 | 1.2:+86258798 | None:intergenic |
| TGTTTGATAAAAGAAGTTGG+AGG | 0.669703 | 1.2:+86259373 | None:intergenic |
| GAAGTTGGAGGAAGGCATCG+AGG | 0.706960 | 1.2:+86259385 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGTTGCATTAATTAAAAA+TGG | + | chr1.2:86259010-86259029 | None:intergenic | 15.0% |
| !!! | TACTACTTTTCATTTCATTT+AGG | - | chr1.2:86259192-86259211 | MS.gene74220:intron | 20.0% |
| ! | ATATCATATGATATGAATGC+AGG | - | chr1.2:86258910-86258929 | MS.gene74220:CDS | 25.0% |
| ! | GTTTGTTTGATAAAAGAAGT+TGG | + | chr1.2:86258757-86258776 | None:intergenic | 25.0% |
| !! | ACTTCTTTTATCAAACAAAC+AGG | - | chr1.2:86258757-86258776 | MS.gene74220:CDS | 25.0% |
| ACATACATACATACATACCT+TGG | + | chr1.2:86258857-86258876 | None:intergenic | 30.0% | |
| ACTGAATGTCCTAATTGTTA+TGG | - | chr1.2:86259285-86259304 | MS.gene74220:CDS | 30.0% | |
| CTGCTTGATAAGATGTATAA+TGG | - | chr1.2:86259393-86259412 | MS.gene74220:CDS | 30.0% | |
| TGATTATGAGAAAAGAGAAG+AGG | + | chr1.2:86259107-86259126 | None:intergenic | 30.0% | |
| TGTTTGATAAAAGAAGTTGG+AGG | + | chr1.2:86258754-86258773 | None:intergenic | 30.0% | |
| TTTCATTTAGGTGATATGTG+TGG | - | chr1.2:86259204-86259223 | MS.gene74220:intron | 30.0% | |
| ! | CTTTTATCAAACAAACAGGA+AGG | - | chr1.2:86258761-86258780 | MS.gene74220:CDS | 30.0% |
| ATACATACATACCTTGGAGT+TGG | + | chr1.2:86258851-86258870 | None:intergenic | 35.0% | |
| CAAACAATAAAGGTCTTCTC+AGG | - | chr1.2:86259352-86259371 | MS.gene74220:CDS | 35.0% | |
| CATCTTATCAAGCAGTTTCT+TGG | + | chr1.2:86259388-86259407 | None:intergenic | 35.0% | |
| CTGAGAAGACCTTTATTGTT+TGG | + | chr1.2:86259354-86259373 | None:intergenic | 35.0% | |
| GAAAATGGAAAGCTCTTAAC+AGG | - | chr1.2:86259235-86259254 | MS.gene74220:intron | 35.0% | |
| TGATAAAAGAAGTTGGAGGA+AGG | + | chr1.2:86258750-86258769 | None:intergenic | 35.0% | |
| TTTCCTCGACCATAACAATT+AGG | + | chr1.2:86259297-86259316 | None:intergenic | 35.0% | |
| ACAATAAAGGTCTTCTCAGG+AGG | - | chr1.2:86259355-86259374 | MS.gene74220:CDS | 40.0% | |
| ACACCAGTTCCTAAACAAAC+AGG | + | chr1.2:86259330-86259349 | None:intergenic | 40.0% | |
| ACTGGTGTGCCAAACAATAA+AGG | - | chr1.2:86259342-86259361 | MS.gene74220:CDS | 40.0% | |
| CACCAGTTCCTAAACAAACA+GGG | + | chr1.2:86259329-86259348 | None:intergenic | 40.0% | |
| CATTTAGGTGATATGTGTGG+TGG | - | chr1.2:86259207-86259226 | MS.gene74220:intron | 40.0% | |
| GCCTGGAAACAACATTTACA+GGG | + | chr1.2:86258958-86258977 | None:intergenic | 40.0% | |
| GGAAGAAGAAGAAGAAGAAG+TGG | - | chr1.2:86258931-86258950 | MS.gene74220:intron | 40.0% | |
| TCCACTCTTCCTCAAAATCA+AGG | - | chr1.2:86258805-86258824 | MS.gene74220:CDS | 40.0% | |
| TGTCCTAATTGTTATGGTCG+AGG | - | chr1.2:86259291-86259310 | MS.gene74220:CDS | 40.0% | |
| ! | ACATTTAAGACATTGACGCC+TGG | + | chr1.2:86258975-86258994 | None:intergenic | 40.0% |
| !! | TCCCTGTAAATGTTGTTTCC+AGG | - | chr1.2:86258954-86258973 | MS.gene74220:intron | 40.0% |
| !! | TCCTTGATTTTGAGGAAGAG+TGG | + | chr1.2:86258809-86258828 | None:intergenic | 40.0% |
| ACATACCTTGGAGTTGGAAG+AGG | + | chr1.2:86258845-86258864 | None:intergenic | 45.0% | |
| ACCTGGAGGTTTATCAAGAG+CGG | + | chr1.2:86259044-86259063 | None:intergenic | 45.0% | |
| CGCCTGGAAACAACATTTAC+AGG | + | chr1.2:86258959-86258978 | None:intergenic | 45.0% | |
| CTTGTCTGCCCTGTTTGTTT+AGG | - | chr1.2:86259318-86259337 | MS.gene74220:CDS | 45.0% | |
| TGCCCTGTTTGTTTAGGAAC+TGG | - | chr1.2:86259324-86259343 | MS.gene74220:CDS | 45.0% | |
| TGTGTGGTGGTACAGGAAAA+TGG | - | chr1.2:86259220-86259239 | MS.gene74220:intron | 45.0% | |
| ! | GAGTGGTGTCCTTGATTTTG+AGG | + | chr1.2:86258817-86258836 | None:intergenic | 45.0% |
| ! | TGCAGAAATTGTGGTGGAAG+TGG | - | chr1.2:86259064-86259083 | MS.gene74220:CDS | 45.0% |
| ACCGCTCTTGATAAACCTCC+AGG | - | chr1.2:86259040-86259059 | MS.gene74220:intron | 50.0% | |
| AGTTGGAAGAGGAGGAAGAG+TGG | + | chr1.2:86258834-86258853 | None:intergenic | 50.0% | |
| CCACAATTTCTGCAACCTGG+AGG | + | chr1.2:86259058-86259077 | None:intergenic | 50.0% | |
| CCACCACAATTTCTGCAACC+TGG | + | chr1.2:86259061-86259080 | None:intergenic | 50.0% | |
| CCAGGTTGCAGAAATTGTGG+TGG | - | chr1.2:86259058-86259077 | MS.gene74220:CDS | 50.0% | |
| CCTCCAGGTTGCAGAAATTG+TGG | - | chr1.2:86259055-86259074 | MS.gene74220:CDS | 50.0% | |
| GGTGATATGTGTGGTGGTAC+AGG | - | chr1.2:86259213-86259232 | MS.gene74220:intron | 50.0% | |
| TACCTTGGAGTTGGAAGAGG+AGG | + | chr1.2:86258842-86258861 | None:intergenic | 50.0% | |
| TCAAGCAGTTTCTTGGCGTC+AGG | + | chr1.2:86259381-86259400 | None:intergenic | 50.0% | |
| TTCCTCCTCTTCCAACTCCA+AGG | - | chr1.2:86258837-86258856 | MS.gene74220:CDS | 50.0% | |
| !! | GAAGTTGGAGGAAGGCATCG+AGG | + | chr1.2:86258742-86258761 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 86258709 | 86259437 | 86258709 | ID=MS.gene74220 |
| chr1.2 | mRNA | 86258709 | 86259437 | 86258709 | ID=MS.gene74220.t1;Parent=MS.gene74220 |
| chr1.2 | exon | 86259256 | 86259437 | 86259256 | ID=MS.gene74220.t1.exon1;Parent=MS.gene74220.t1 |
| chr1.2 | CDS | 86259256 | 86259437 | 86259256 | ID=cds.MS.gene74220.t1;Parent=MS.gene74220.t1 |
| chr1.2 | exon | 86259049 | 86259170 | 86259049 | ID=MS.gene74220.t1.exon2;Parent=MS.gene74220.t1 |
| chr1.2 | CDS | 86259049 | 86259170 | 86259049 | ID=cds.MS.gene74220.t1;Parent=MS.gene74220.t1 |
| chr1.2 | exon | 86258709 | 86258932 | 86258709 | ID=MS.gene74220.t1.exon3;Parent=MS.gene74220.t1 |
| chr1.2 | CDS | 86258709 | 86258932 | 86258709 | ID=cds.MS.gene74220.t1;Parent=MS.gene74220.t1 |
| Gene Sequence |
| Protein sequence |
