AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75523


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75523.t1 MTR_3g086590 90.431 209 5 4 1 209 1 194 2.59e-133 372
MS.gene75523.t1 MTR_4g104680 58.519 135 54 2 75 209 58 190 1.43e-47 166
MS.gene75523.t1 MTR_1g047990 34.109 129 82 1 75 203 78 203 9.36e-17 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75523.t1 AT1G67810 51.064 188 84 5 25 209 27 209 1.13e-56 180
MS.gene75523.t1 AT5G50210 48.889 135 67 1 75 209 87 219 1.55e-37 138
MS.gene75523.t1 AT4G26500 35.659 129 80 1 75 203 86 211 1.82e-16 77.0

Find 69 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACAAAGGATTTAATTTA+AGG 0.108182 3.3:+72266697 MS.gene75523:CDS
GAGAAGGAGGGAGGGTTAAT+AGG 0.172032 3.3:-72266138 None:intergenic
AACCCGGTCGGACCGGTCTA+AGG 0.264863 3.3:-72266401 None:intergenic
ATAATGTTAAAGCACGTTCT+AGG 0.299172 3.3:+72266635 MS.gene75523:CDS
CAGGTTGTTCGACAGATGTT+TGG 0.324183 3.3:+72266440 MS.gene75523:CDS
AGAAGGAGGGAGGGTTAATA+GGG 0.328208 3.3:-72266137 None:intergenic
GGTTCTGTTGGTGTCTTGTT+TGG 0.336055 3.3:+72266533 MS.gene75523:CDS
AACGGCGGCGGCGTTAAGTT+TGG 0.345006 3.3:-72266182 None:intergenic
TAACGTCGAGAAAGATGTTT+TGG 0.349720 3.3:+72266588 MS.gene75523:CDS
AGACCGGTCCGACCGGGTTC+CGG 0.370178 3.3:+72266405 MS.gene75523:CDS
CTCCCTTAGACCGGTCCGAC+CGG 0.409468 3.3:+72266398 MS.gene75523:CDS
CCGACCGGGTTCCGGAGAAC+CGG 0.419653 3.3:+72266413 MS.gene75523:CDS
TTGGTTGGTATGAATGTAGT+AGG 0.419990 3.3:+72266607 MS.gene75523:CDS
CAACCTGTCACCCGGTTCTC+CGG 0.423018 3.3:-72266424 None:intergenic
GTTGAAGGGAGGAGGGTTAA+CGG 0.424094 3.3:-72266233 None:intergenic
GTTGGGAATGGAAATGGAAA+TGG 0.446723 3.3:-72266097 None:intergenic
AGGTTGTTCGACAGATGTTT+GGG 0.451276 3.3:+72266441 MS.gene75523:CDS
TGTCTTGTTTGGATGTTTGA+CGG 0.452263 3.3:+72266544 MS.gene75523:CDS
AAGGAGAAGGAGAAGGAGGG+AGG 0.455202 3.3:-72266147 None:intergenic
TGAGAAGGAGAAGGAGAAGG+AGG 0.464083 3.3:-72266151 None:intergenic
GTTCCGGAGAACCGGGTGAC+AGG 0.466591 3.3:+72266421 MS.gene75523:CDS
GTGGAACTTGCCGTAGAAGT+TGG 0.470590 3.3:-72266115 None:intergenic
GTAGAAGTTGGGAATGGAAA+TGG 0.471303 3.3:-72266103 None:intergenic
TGGTTGGTATGAATGTAGTA+GGG 0.480747 3.3:+72266608 MS.gene75523:CDS
AGGAGAAGGAGAAGGAGGGA+GGG 0.480933 3.3:-72266146 None:intergenic
TCTCCTACCTTTGTCTATGC+GGG 0.481076 3.3:-72266470 None:intergenic
TGGAACTTGCCGTAGAAGTT+GGG 0.482966 3.3:-72266114 None:intergenic
ATTTCCATTCCCAACTTCTA+CGG 0.492903 3.3:+72266105 MS.gene75523:CDS
TCTCCGGAACCCGGTCGGAC+CGG 0.495621 3.3:-72266408 None:intergenic
CTTTATTGCCTCCCTTAGAC+CGG 0.497345 3.3:+72266389 MS.gene75523:CDS
TCGACGTTAAGCACTTCCTC+AGG 0.504341 3.3:-72266574 None:intergenic
TTCGACAGATGTTTGGGTGG+TGG 0.504447 3.3:+72266447 MS.gene75523:CDS
TCCCTTAGACCGGTCCGACC+GGG 0.506418 3.3:+72266399 MS.gene75523:CDS
AATGTGTTGTTGAAGGGAGG+AGG 0.512295 3.3:-72266241 None:intergenic
ATGTGTTGTTGAAGGGAGGA+GGG 0.516128 3.3:-72266240 None:intergenic
GAGAAGGAGAAGGAGAAGGA+GGG 0.521011 3.3:-72266150 None:intergenic
GTTTCAAGCAGACAGCGATT+CGG 0.525213 3.3:+72266498 MS.gene75523:CDS
GATGTTAATGTGTTGTTGAA+GGG 0.535061 3.3:-72266247 None:intergenic
TTCTCCTACCTTTGTCTATG+CGG 0.535727 3.3:-72266471 None:intergenic
GGCGGCGGCGTTAAGTTTGG+AGG 0.536844 3.3:-72266179 None:intergenic
CGACCGGGTTCCGGAGAACC+GGG 0.537863 3.3:+72266414 MS.gene75523:CDS
TGATGTTAATGTGTTGTTGA+AGG 0.542600 3.3:-72266248 None:intergenic
AGGAGGGAGGGTTAATAGGG+TGG 0.544493 3.3:-72266134 None:intergenic
AAATGAGAAGGAGAAGGAGA+AGG 0.545044 3.3:-72266154 None:intergenic
ACCCGGTCGGACCGGTCTAA+GGG 0.546406 3.3:-72266400 None:intergenic
AGTTTGGAGGAGAAATGAGA+AGG 0.551683 3.3:-72266166 None:intergenic
TCATCATCACCGTCATCTCA+CGG 0.561395 3.3:+72266277 MS.gene75523:CDS
GTTTGACGGCGCGGAGCCTG+AGG 0.562974 3.3:+72266558 MS.gene75523:CDS
TCACCCGGTTCTCCGGAACC+CGG 0.567326 3.3:-72266417 None:intergenic
GAGGAGAAATGAGAAGGAGA+AGG 0.570126 3.3:-72266160 None:intergenic
CTGTCGAACAACCTGTCACC+CGG 0.571190 3.3:-72266432 None:intergenic
GGTTAACGGAGAGTTTGAGA+TGG 0.571772 3.3:-72266219 None:intergenic
TAGACAAAGGTAGGAGAATG+AGG 0.573501 3.3:+72266476 MS.gene75523:CDS
AGATATCGAAGGGGTTCTGT+TGG 0.579062 3.3:+72266521 MS.gene75523:CDS
GTCGATGGGTTCGGAGAGAG+AGG 0.581664 3.3:-72266338 None:intergenic
CCGGTTCTCCGGAACCCGGT+CGG 0.599576 3.3:-72266413 None:intergenic
AGCGATTCGGAGATATCGAA+GGG 0.599661 3.3:+72266511 MS.gene75523:CDS
CAGCGATTCGGAGATATCGA+AGG 0.616023 3.3:+72266510 MS.gene75523:CDS
GGAGAGAGAGGCAAATTCGA+TGG 0.622274 3.3:-72266326 None:intergenic
CTTGCCGTAGAAGTTGGGAA+TGG 0.632430 3.3:-72266109 None:intergenic
TTGTTCGACAGATGTTTGGG+TGG 0.634015 3.3:+72266444 MS.gene75523:CDS
TGGCCCGCATAGACAAAGGT+AGG 0.636995 3.3:+72266467 MS.gene75523:CDS
CGGTCGGACCGGTCTAAGGG+AGG 0.638651 3.3:-72266397 None:intergenic
GTTAATGTGTTGTTGAAGGG+AGG 0.644344 3.3:-72266244 None:intergenic
GTGGTGGCCCGCATAGACAA+AGG 0.655112 3.3:+72266463 MS.gene75523:CDS
TCAGTGACACCGTGAGATGA+CGG 0.694277 3.3:-72266286 None:intergenic
TGTTTGGATGTTTGACGGCG+CGG 0.699310 3.3:+72266549 MS.gene75523:CDS
CACGTTCTAGGATTAACACG+TGG 0.721590 3.3:+72266647 MS.gene75523:CDS
GCGATTCGGAGATATCGAAG+GGG 0.771683 3.3:+72266512 MS.gene75523:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTAATAGTCTTTTTACT+CGG - chr3.3:72266363-72266382 None:intergenic 20.0%
!!! ATGGATTTGTTTTGAAGTAA+CGG - chr3.3:72266203-72266222 None:intergenic 25.0%
ATAATGTTAAAGCACGTTCT+AGG + chr3.3:72266635-72266654 MS.gene75523:CDS 30.0%
! GATGTTAATGTGTTGTTGAA+GGG - chr3.3:72266250-72266269 None:intergenic 30.0%
! TGATGTTAATGTGTTGTTGA+AGG - chr3.3:72266251-72266270 None:intergenic 30.0%
ATTTCCATTCCCAACTTCTA+CGG + chr3.3:72266105-72266124 MS.gene75523:CDS 35.0%
! ATCCTTTGTAGCTTTTTGCA+TGG - chr3.3:72266689-72266708 None:intergenic 35.0%
! TAACGTCGAGAAAGATGTTT+TGG + chr3.3:72266588-72266607 MS.gene75523:CDS 35.0%
! TGGTTGGTATGAATGTAGTA+GGG + chr3.3:72266608-72266627 MS.gene75523:CDS 35.0%
! TTGGTTGGTATGAATGTAGT+AGG + chr3.3:72266607-72266626 MS.gene75523:CDS 35.0%
!! TGTCTTGTTTGGATGTTTGA+CGG + chr3.3:72266544-72266563 MS.gene75523:CDS 35.0%
!!! GATTTGTTTTGAAGTAACGG+CGG - chr3.3:72266200-72266219 None:intergenic 35.0%
AAATGAGAAGGAGAAGGAGA+AGG - chr3.3:72266157-72266176 None:intergenic 40.0%
AGGTTGTTCGACAGATGTTT+GGG + chr3.3:72266441-72266460 MS.gene75523:CDS 40.0%
AGTTTGGAGGAGAAATGAGA+AGG - chr3.3:72266169-72266188 None:intergenic 40.0%
GTAGAAGTTGGGAATGGAAA+TGG - chr3.3:72266106-72266125 None:intergenic 40.0%
GTTGGGAATGGAAATGGAAA+TGG - chr3.3:72266100-72266119 None:intergenic 40.0%
TAGACAAAGGTAGGAGAATG+AGG + chr3.3:72266476-72266495 MS.gene75523:CDS 40.0%
TGCCATGCAAAAAGCTACAA+AGG + chr3.3:72266684-72266703 MS.gene75523:CDS 40.0%
TTCTCCTACCTTTGTCTATG+CGG - chr3.3:72266474-72266493 None:intergenic 40.0%
! AGTCTTTTTACTCGGTCGAT+GGG - chr3.3:72266355-72266374 None:intergenic 40.0%
! GTTAATGTGTTGTTGAAGGG+AGG - chr3.3:72266247-72266266 None:intergenic 40.0%
! TAGTCTTTTTACTCGGTCGA+TGG - chr3.3:72266356-72266375 None:intergenic 40.0%
!!! GTCGAGAAAGATGTTTTGGT+TGG + chr3.3:72266592-72266611 MS.gene75523:CDS 40.0%
AGAAGGAGGGAGGGTTAATA+GGG - chr3.3:72266140-72266159 None:intergenic 45.0%
AGATATCGAAGGGGTTCTGT+TGG + chr3.3:72266521-72266540 MS.gene75523:CDS 45.0%
CAGGTTGTTCGACAGATGTT+TGG + chr3.3:72266440-72266459 MS.gene75523:CDS 45.0%
CTTTATTGCCTCCCTTAGAC+CGG + chr3.3:72266389-72266408 MS.gene75523:CDS 45.0%
GAGGAGAAATGAGAAGGAGA+AGG - chr3.3:72266163-72266182 None:intergenic 45.0%
GGTTAACGGAGAGTTTGAGA+TGG - chr3.3:72266222-72266241 None:intergenic 45.0%
GTTTCAAGCAGACAGCGATT+CGG + chr3.3:72266498-72266517 MS.gene75523:CDS 45.0%
TCATCATCACCGTCATCTCA+CGG + chr3.3:72266277-72266296 MS.gene75523:CDS 45.0%
TCTCCTACCTTTGTCTATGC+GGG - chr3.3:72266473-72266492 None:intergenic 45.0%
TGGAACTTGCCGTAGAAGTT+GGG - chr3.3:72266117-72266136 None:intergenic 45.0%
TTGTTCGACAGATGTTTGGG+TGG + chr3.3:72266444-72266463 MS.gene75523:CDS 45.0%
! AATGTGTTGTTGAAGGGAGG+AGG - chr3.3:72266244-72266263 None:intergenic 45.0%
! AGCGATTCGGAGATATCGAA+GGG + chr3.3:72266511-72266530 MS.gene75523:CDS 45.0%
! ATGTGTTGTTGAAGGGAGGA+GGG - chr3.3:72266243-72266262 None:intergenic 45.0%
! GGTTCTGTTGGTGTCTTGTT+TGG + chr3.3:72266533-72266552 MS.gene75523:CDS 45.0%
!! CACGTTCTAGGATTAACACG+TGG + chr3.3:72266647-72266666 MS.gene75523:CDS 45.0%
!! TTTTACTCGGTCGATGGGTT+CGG - chr3.3:72266350-72266369 None:intergenic 45.0%
!!! TTGTTTTGAAGTAACGGCGG+CGG - chr3.3:72266197-72266216 None:intergenic 45.0%
CTTGCCGTAGAAGTTGGGAA+TGG - chr3.3:72266112-72266131 None:intergenic 50.0%
GAGAAGGAGAAGGAGAAGGA+GGG - chr3.3:72266153-72266172 None:intergenic 50.0%
GAGAAGGAGGGAGGGTTAAT+AGG - chr3.3:72266141-72266160 None:intergenic 50.0%
GGAGAGAGAGGCAAATTCGA+TGG - chr3.3:72266329-72266348 None:intergenic 50.0%
GTGGAACTTGCCGTAGAAGT+TGG - chr3.3:72266118-72266137 None:intergenic 50.0%
GTTGAAGGGAGGAGGGTTAA+CGG - chr3.3:72266236-72266255 None:intergenic 50.0%
TCAGTGACACCGTGAGATGA+CGG - chr3.3:72266289-72266308 None:intergenic 50.0%
TCGACGTTAAGCACTTCCTC+AGG - chr3.3:72266577-72266596 None:intergenic 50.0%
TGAGAAGGAGAAGGAGAAGG+AGG - chr3.3:72266154-72266173 None:intergenic 50.0%
TGTTTGGATGTTTGACGGCG+CGG + chr3.3:72266549-72266568 MS.gene75523:CDS 50.0%
TTCGACAGATGTTTGGGTGG+TGG + chr3.3:72266447-72266466 MS.gene75523:CDS 50.0%
! CAGCGATTCGGAGATATCGA+AGG + chr3.3:72266510-72266529 MS.gene75523:CDS 50.0%
! GCGATTCGGAGATATCGAAG+GGG + chr3.3:72266512-72266531 MS.gene75523:CDS 50.0%
AAGGAGAAGGAGAAGGAGGG+AGG - chr3.3:72266150-72266169 None:intergenic 55.0%
AGGAGAAGGAGAAGGAGGGA+GGG - chr3.3:72266149-72266168 None:intergenic 55.0%
AGGAGGGAGGGTTAATAGGG+TGG - chr3.3:72266137-72266156 None:intergenic 55.0%
CTGTCGAACAACCTGTCACC+CGG - chr3.3:72266435-72266454 None:intergenic 55.0%
TGGCCCGCATAGACAAAGGT+AGG + chr3.3:72266467-72266486 MS.gene75523:CDS 55.0%
AACGGCGGCGGCGTTAAGTT+TGG - chr3.3:72266185-72266204 None:intergenic 60.0%
CAACCTGTCACCCGGTTCTC+CGG - chr3.3:72266427-72266446 None:intergenic 60.0%
GTGGTGGCCCGCATAGACAA+AGG + chr3.3:72266463-72266482 MS.gene75523:CDS 60.0%
! GTCGATGGGTTCGGAGAGAG+AGG - chr3.3:72266341-72266360 None:intergenic 60.0%
AACCCGGTCGGACCGGTCTA+AGG - chr3.3:72266404-72266423 None:intergenic 65.0%
ACCCGGTCGGACCGGTCTAA+GGG - chr3.3:72266403-72266422 None:intergenic 65.0%
CTCCCTTAGACCGGTCCGAC+CGG + chr3.3:72266398-72266417 MS.gene75523:CDS 65.0%
GGCGGCGGCGTTAAGTTTGG+AGG - chr3.3:72266182-72266201 None:intergenic 65.0%
GTTCCGGAGAACCGGGTGAC+AGG + chr3.3:72266421-72266440 MS.gene75523:CDS 65.0%
TCACCCGGTTCTCCGGAACC+CGG - chr3.3:72266420-72266439 None:intergenic 65.0%
TCCCTTAGACCGGTCCGACC+GGG + chr3.3:72266399-72266418 MS.gene75523:CDS 65.0%
AGACCGGTCCGACCGGGTTC+CGG + chr3.3:72266405-72266424 MS.gene75523:CDS 70.0%
CCGACCGGGTTCCGGAGAAC+CGG + chr3.3:72266413-72266432 MS.gene75523:CDS 70.0%
CCGGTTCTCCGGAACCCGGT+CGG - chr3.3:72266416-72266435 None:intergenic 70.0%
CGACCGGGTTCCGGAGAACC+GGG + chr3.3:72266414-72266433 MS.gene75523:CDS 70.0%
CGGTCGGACCGGTCTAAGGG+AGG - chr3.3:72266400-72266419 None:intergenic 70.0%
GTTTGACGGCGCGGAGCCTG+AGG + chr3.3:72266558-72266577 MS.gene75523:CDS 70.0%
TCTCCGGAACCCGGTCGGAC+CGG - chr3.3:72266411-72266430 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 72266088 72266717 72266088 ID=MS.gene75523
chr3.3 mRNA 72266088 72266717 72266088 ID=MS.gene75523.t1;Parent=MS.gene75523
chr3.3 exon 72266088 72266717 72266088 ID=MS.gene75523.t1.exon1;Parent=MS.gene75523.t1
chr3.3 CDS 72266088 72266717 72266088 ID=cds.MS.gene75523.t1;Parent=MS.gene75523.t1
Gene Sequence

>MS.gene75523

ATGCTATATCCATTTCCATTTCCATTCCCAACTTCTACGGCAAGTTCCACCCTATTAACCCTCCCTCCTTCTCCTTCTCCTTCTCATTTCTCCTCCAAACTTAACGCCGCCGCCGTTACTTCAAAACAAATCCATCTCAAACTCTCCGTTAACCCTCCTCCCTTCAACAACACATTAACATCATCTTTTTCATCATCACCGTCATCTCACGGTGTCACTGACAAGCTCAACAATCTCACCATCGAATTTGCCTCTCTCTCCGAACCCATCGACCGAGTAAAAAGACTATTACATTACGCATCTTTATTGCCTCCCTTAGACCGGTCCGACCGGGTTCCGGAGAACCGGGTGACAGGTTGTTCGACAGATGTTTGGGTGGTGGCCCGCATAGACAAAGGTAGGAGAATGAGGTTTCAAGCAGACAGCGATTCGGAGATATCGAAGGGGTTCTGTTGGTGTCTTGTTTGGATGTTTGACGGCGCGGAGCCTGAGGAAGTGCTTAACGTCGAGAAAGATGTTTTGGTTGGTATGAATGTAGTAGGGTTGAATAATGTTAAAGCACGTTCTAGGATTAACACGTGGCATAATGTTTTCTTTGCCATGCAAAAAGCTACAAAGGATTTAATTTAA

Protein sequence

>MS.gene75523.t1

MLYPFPFPFPTSTASSTLLTLPPSPSPSHFSSKLNAAAVTSKQIHLKLSVNPPPFNNTLTSSFSSSPSSHGVTDKLNNLTIEFASLSEPIDRVKRLLHYASLLPPLDRSDRVPENRVTGCSTDVWVVARIDKGRRMRFQADSDSEISKGFCWCLVWMFDGAEPEEVLNVEKDVLVGMNVVGLNNVKARSRINTWHNVFFAMQKATKDLI