AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79127


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79127.t1 MTR_4g123670 83.333 48 8 0 5 52 472 519 2.34e-19 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACACGACTTCCCTTGTTTC+AGG 0.153942 3.1:+68695912 None:intergenic
AAACTGCTTCACTTCACTAA+TGG 0.340507 3.1:-68695876 MS.gene79127:CDS
TTGTAATTCTTTCAACTGAA+TGG 0.353975 3.1:+68695847 None:intergenic
GGATGAAGATGGCGTGGATG+AGG 0.450681 3.1:-68695801 MS.gene79127:CDS
ATTAACCTCATCATAATCTT+CGG 0.465947 3.1:+68695760 None:intergenic
TGGGAAAGGAGATGAAGTAG+AGG 0.496213 3.1:-68695822 MS.gene79127:CDS
CAGTTGAAAGAATTACAAGA+TGG 0.531894 3.1:-68695842 MS.gene79127:CDS
GATGAAGTAGAGGATGAAGA+TGG 0.543029 3.1:-68695812 MS.gene79127:CDS
CTGAGCAACTCCTGAAACAA+GGG 0.557999 3.1:-68695922 MS.gene79127:CDS
AGTTGAAAGAATTACAAGAT+GGG 0.566152 3.1:-68695841 MS.gene79127:CDS
CTTCTCCACACAAACCTCCA+TGG 0.568447 3.1:+68696033 None:intergenic
CTGTTTAAATTAGGACTCTG+AGG 0.574975 3.1:-68695599 MS.gene79127:intron
ACTGAGCAACTCCTGAAACA+AGG 0.580659 3.1:-68695923 MS.gene79127:CDS
AAAGAATTACAAGATGGGAA+AGG 0.585242 3.1:-68695836 MS.gene79127:CDS
CTGCTTCACTTCACTAATGG+AGG 0.589139 3.1:-68695873 MS.gene79127:CDS
ATGAGAGGGAGAATGAGCCA+TGG 0.616733 3.1:-68696050 MS.gene79127:CDS
AAAAGCCATGGACAAATGAG+AGG 0.628002 3.1:-68696065 None:intergenic
AGTAGAGGATGAAGATGGCG+TGG 0.645857 3.1:-68695807 MS.gene79127:CDS
AGAGGGAGAATGAGCCATGG+AGG 0.663372 3.1:-68696047 MS.gene79127:intron
CTGAAACAAGGGAAGTCGTG+TGG 0.664589 3.1:-68695911 MS.gene79127:CDS
AAAGCCATGGACAAATGAGA+GGG 0.669427 3.1:-68696064 None:intergenic
TGACTCCGAAGATTATGATG+AGG 0.676696 3.1:-68695765 MS.gene79127:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGTTGAAAGAATTACAAGAT+GGG - chr3.1:68695791-68695810 MS.gene79127:CDS 25.0%
! ATTAACCTCATCATAATCTT+CGG + chr3.1:68695875-68695894 None:intergenic 25.0%
! TTGTAATTCTTTCAACTGAA+TGG + chr3.1:68695788-68695807 None:intergenic 25.0%
AAAGAATTACAAGATGGGAA+AGG - chr3.1:68695796-68695815 MS.gene79127:CDS 30.0%
CAGTTGAAAGAATTACAAGA+TGG - chr3.1:68695790-68695809 MS.gene79127:CDS 30.0%
TTAACAGTTCTTAATCCTGT+GGG - chr3.1:68696009-68696028 MS.gene79127:intron 30.0%
TTTAACAGTTCTTAATCCTG+TGG - chr3.1:68696008-68696027 MS.gene79127:intron 30.0%
! TATATCTCCTTTTGTCTACA+AGG - chr3.1:68695942-68695961 MS.gene79127:CDS 30.0%
!! TAACTTTTCCGTTTTAGTGA+TGG - chr3.1:68695672-68695691 MS.gene79127:intron 30.0%
AAACTGCTTCACTTCACTAA+TGG - chr3.1:68695756-68695775 MS.gene79127:intron 35.0%
CTGTTTAAATTAGGACTCTG+AGG - chr3.1:68696033-68696052 MS.gene79127:intron 35.0%
ACCTAGTGCCATCACTAAAA+CGG + chr3.1:68695683-68695702 None:intergenic 40.0%
CACAGAACCTTGTAGACAAA+AGG + chr3.1:68695952-68695971 None:intergenic 40.0%
CCTAATTTAAACAGACCCAC+AGG + chr3.1:68696027-68696046 None:intergenic 40.0%
CCTGTGGGTCTGTTTAAATT+AGG - chr3.1:68696024-68696043 MS.gene79127:intron 40.0%
GATGAAGTAGAGGATGAAGA+TGG - chr3.1:68695820-68695839 MS.gene79127:CDS 40.0%
TGACTCCGAAGATTATGATG+AGG - chr3.1:68695867-68695886 MS.gene79127:CDS 40.0%
ACTGAGCAACTCCTGAAACA+AGG - chr3.1:68695709-68695728 MS.gene79127:intron 45.0%
CTGAGCAACTCCTGAAACAA+GGG - chr3.1:68695710-68695729 MS.gene79127:intron 45.0%
CTGCTTCACTTCACTAATGG+AGG - chr3.1:68695759-68695778 MS.gene79127:intron 45.0%
TGGGAAAGGAGATGAAGTAG+AGG - chr3.1:68695810-68695829 MS.gene79127:CDS 45.0%
!! TCCGTTTTAGTGATGGCACT+AGG - chr3.1:68695679-68695698 MS.gene79127:intron 45.0%
AGTAGAGGATGAAGATGGCG+TGG - chr3.1:68695825-68695844 MS.gene79127:CDS 50.0%
ATGAGAGGGAGAATGAGCCA+TGG - chr3.1:68695582-68695601 MS.gene79127:CDS 50.0%
ATGAGCCATGGAGGTTTGTG+TGG - chr3.1:68695594-68695613 MS.gene79127:intron 50.0%
CTGAAACAAGGGAAGTCGTG+TGG - chr3.1:68695721-68695740 MS.gene79127:intron 50.0%
CTTCTCCACACAAACCTCCA+TGG + chr3.1:68695602-68695621 None:intergenic 50.0%
! CACACGACTTCCCTTGTTTC+AGG + chr3.1:68695723-68695742 None:intergenic 50.0%
AGAGGGAGAATGAGCCATGG+AGG - chr3.1:68695585-68695604 MS.gene79127:CDS 55.0%
GGATGAAGATGGCGTGGATG+AGG - chr3.1:68695831-68695850 MS.gene79127:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 68695582 68696072 68695582 ID=MS.gene79127
chr3.1 mRNA 68695582 68696072 68695582 ID=MS.gene79127.t1;Parent=MS.gene79127
chr3.1 exon 68696048 68696072 68696048 ID=MS.gene79127.t1.exon1;Parent=MS.gene79127.t1
chr3.1 CDS 68696048 68696072 68696048 ID=cds.MS.gene79127.t1;Parent=MS.gene79127.t1
chr3.1 exon 68695766 68695965 68695766 ID=MS.gene79127.t1.exon2;Parent=MS.gene79127.t1
chr3.1 CDS 68695766 68695965 68695766 ID=cds.MS.gene79127.t1;Parent=MS.gene79127.t1
chr3.1 exon 68695582 68695608 68695582 ID=MS.gene79127.t1.exon3;Parent=MS.gene79127.t1
chr3.1 CDS 68695582 68695608 68695582 ID=cds.MS.gene79127.t1;Parent=MS.gene79127.t1
Gene Sequence

>MS.gene79127

ATGAGAGGGAGAATGAGCCATGGAGGTTTGTGTGGAGAAGACGATGAGTTTGCTTCTTCTTCTTTTTAATGTCTTTTCAGCATTTTTTTTTAACTTTTCCGTTTTAGTGATGGCACTAGGTCGATTGACTGAGCAACTCCTGAAACAAGGGAAGTCGTGTGGTGATTCAATTCAAAACTGCTTCACTTCACTAATGGAGGTATCCATTCAGTTGAAAGAATTACAAGATGGGAAAGGAGATGAAGTAGAGGATGAAGATGGCGTGGATGAGGATAGTGACGATGATGACTCCGAAGATTATGATGAGGTTAATCTTTACAGTTTCTTCATGTATATCAGTTTTTAGTGTTTTCGTATACTTATATCTCCTTTTGTCTACAAGGTTCTGTGATTAACATGTTTTCTTGCTTACTATGTTTATTAATATTTAACAGTTCTTAATCCTGTGGGTCTGTTTAAATTAGGACTCTGAGGTTGAAGAATATGAATAA

Protein sequence

>MS.gene79127.t1

MRGRMSHGVMALGRLTEQLLKQGKSCGDSIQNCFTSLMEVSIQLKELQDGKGDEVEDEDGVDEDSDDDDSEDYDEDSEVEEYE