AlfalfaGEDB | MS.gene83729

M. sativa cultivar XinJiangDaYe / MS.gene83729

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83729.t1	KEH29461.1	58.1	86	26	1	1	76	18	103	1.50E-16	95.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83729.t1	A0A072UJ67	58.1	86	26	1	1	76	18	103	1.1e-16	95.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049320	MS.gene83729	-0.805315	1.42E-49	-1.69E-46
MS.gene049611	MS.gene83729	0.825619	4.25E-54	-1.69E-46
MS.gene049634	MS.gene83729	0.830627	2.64E-55	-1.69E-46
MS.gene050381	MS.gene83729	0.819384	1.19E-52	-1.69E-46
MS.gene050504	MS.gene83729	-0.807136	5.86E-50	-1.69E-46
MS.gene050547	MS.gene83729	0.80686	6.70E-50	-1.69E-46
MS.gene050612	MS.gene83729	-0.830525	2.80E-55	-1.69E-46
MS.gene050883	MS.gene83729	0.820047	8.43E-53	-1.69E-46
MS.gene051044	MS.gene83729	0.808648	2.79E-50	-1.69E-46
MS.gene051127	MS.gene83729	0.825643	4.19E-54	-1.69E-46
MS.gene051138	MS.gene83729	0.865385	6.14E-65	-1.69E-46
MS.gene052211	MS.gene83729	0.827611	1.42E-54	-1.69E-46
MS.gene052802	MS.gene83729	0.810457	1.14E-50	-1.69E-46
MS.gene053406	MS.gene83729	0.80089	1.17E-48	-1.69E-46
MS.gene053420	MS.gene83729	0.811954	5.41E-51	-1.69E-46
MS.gene053488	MS.gene83729	0.836088	1.15E-56	-1.69E-46
MS.gene053561	MS.gene83729	0.839056	1.99E-57	-1.69E-46
MS.gene054054	MS.gene83729	0.800561	1.36E-48	-1.69E-46
MS.gene055111	MS.gene83729	0.813696	2.25E-51	-1.69E-46
MS.gene055124	MS.gene83729	0.830746	2.47E-55	-1.69E-46
MS.gene055154	MS.gene83729	0.854176	1.46E-61	-1.69E-46
MS.gene055362	MS.gene83729	-0.805284	1.44E-49	-1.69E-46
MS.gene055502	MS.gene83729	-0.812992	3.21E-51	-1.69E-46
MS.gene055748	MS.gene83729	-0.803708	3.06E-49	-1.69E-46
MS.gene056224	MS.gene83729	0.825068	5.73E-54	-1.69E-46
MS.gene056550	MS.gene83729	0.803419	3.52E-49	-1.69E-46
MS.gene056748	MS.gene83729	0.804359	2.24E-49	-1.69E-46
MS.gene056856	MS.gene83729	0.801708	7.93E-49	-1.69E-46
MS.gene058068	MS.gene83729	0.805856	1.09E-49	-1.69E-46
MS.gene058099	MS.gene83729	0.822152	2.76E-53	-1.69E-46
MS.gene058171	MS.gene83729	0.830989	2.15E-55	-1.69E-46
MS.gene058173	MS.gene83729	0.82332	1.48E-53	-1.69E-46
MS.gene058175	MS.gene83729	0.827538	1.48E-54	-1.69E-46
MS.gene058603	MS.gene83729	0.833447	5.31E-56	-1.69E-46
MS.gene058840	MS.gene83729	0.823291	1.50E-53	-1.69E-46
MS.gene059022	MS.gene83729	-0.812097	5.03E-51	-1.69E-46
MS.gene059677	MS.gene83729	-0.802997	4.30E-49	-1.69E-46
MS.gene059690	MS.gene83729	0.832941	7.09E-56	-1.69E-46
MS.gene059691	MS.gene83729	0.830436	2.94E-55	-1.69E-46
MS.gene059694	MS.gene83729	0.846211	2.50E-59	-1.69E-46
MS.gene059697	MS.gene83729	0.848348	6.49E-60	-1.69E-46
MS.gene059916	MS.gene83729	0.804083	2.56E-49	-1.69E-46
MS.gene06077	MS.gene83729	-0.814351	1.61E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83729.t1	MTR_4g037005	65.116	86	20	1	1	76	18	103	1.47e-34	113
MS.gene83729.t1	MTR_7g028435	62.500	88	21	2	1	76	18	105	5.40e-32	107
MS.gene83729.t1	MTR_4g037005	62.500	88	21	2	1	76	18	105	5.40e-32	107
MS.gene83729.t1	MTR_4g036795	69.697	66	13	1	1	66	18	76	4.60e-27	94.0
MS.gene83729.t1	MTR_4g036990	48.684	76	17	3	1	76	18	71	2.05e-15	64.3
MS.gene83729.t1	MTR_8g070055	43.750	80	28	4	1	76	18	84	2.64e-13	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 12 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGTAGCTTTCTATTCTTA+TGG	0.240515	4.3:-62716478	None:intergenic
CCTCTCTTACATCGCTGATC+TGG	0.242771	4.3:-62716503	None:intergenic
CCCGTTGTACTTAATAAAAC+TGG	0.320363	4.3:+62716554	MS.gene83729:CDS
CTCTCTTACATCGCTGATCT+GGG	0.334871	4.3:-62716502	None:intergenic
AAAGCAACAAGGAACATTCT+TGG	0.424631	4.3:+62716347	MS.gene83729:CDS
CACTTACTTGCATACATGTC+AGG	0.457216	4.3:-62716374	None:intergenic
CCAGATCAGCGATGTAAGAG+AGG	0.582999	4.3:+62716503	MS.gene83729:CDS
CAAAGATAATTGTGGCAACG+TGG	0.625898	4.3:-62716620	None:intergenic
AGATCGATCAAAGATAATTG+TGG	0.646970	4.3:-62716628	None:intergenic
TCTCTTACATCGCTGATCTG+GGG	0.649695	4.3:-62716501	None:intergenic
TGTATTTAATAAAACTCACG+AGG	0.693276	4.3:+62716580	MS.gene83729:CDS
GTTTCTCTACTAAAGCAACA+AGG	0.710702	4.3:+62716336	MS.gene83729:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATATATAATTTGTTCTTTTA+TGG	+	chr4.3:62716442-62716461	MS.gene83729:intron	10.0%
!	TGTATTTAATAAAACTCACG+AGG	+	chr4.3:62716580-62716599	MS.gene83729:CDS	25.0%
!!!	ACCAGTTTTATTAAGTACAA+CGG	-	chr4.3:62716558-62716577	None:intergenic	25.0%
	CAAGTAGCTTTCTATTCTTA+TGG	-	chr4.3:62716481-62716500	None:intergenic	30.0%
!!!	CCAGTTTTATTAAGTACAAC+GGG	-	chr4.3:62716557-62716576	None:intergenic	30.0%
	AAAGCAACAAGGAACATTCT+TGG	+	chr4.3:62716347-62716366	MS.gene83729:CDS	35.0%
	CCCGTTGTACTTAATAAAAC+TGG	+	chr4.3:62716554-62716573	MS.gene83729:CDS	35.0%
	GTTTCTCTACTAAAGCAACA+AGG	+	chr4.3:62716336-62716355	MS.gene83729:CDS	35.0%
	CAAAGATAATTGTGGCAACG+TGG	-	chr4.3:62716623-62716642	None:intergenic	40.0%
	CACTTACTTGCATACATGTC+AGG	-	chr4.3:62716377-62716396	None:intergenic	40.0%
	ACGCAAGCACACAAATGGAA+TGG	-	chr4.3:62716417-62716436	None:intergenic	45.0%
	CGCAAGCACACAAATGGAAT+GGG	-	chr4.3:62716416-62716435	None:intergenic	45.0%
	CTCTCTTACATCGCTGATCT+GGG	-	chr4.3:62716505-62716524	None:intergenic	45.0%
	GCAAAACGCAAGCACACAAA+TGG	-	chr4.3:62716422-62716441	None:intergenic	45.0%
	TCTCTTACATCGCTGATCTG+GGG	-	chr4.3:62716504-62716523	None:intergenic	45.0%
	CCAGATCAGCGATGTAAGAG+AGG	+	chr4.3:62716503-62716522	MS.gene83729:CDS	50.0%
	CCTCTCTTACATCGCTGATC+TGG	-	chr4.3:62716506-62716525	None:intergenic	50.0%
!	TGGCAACGTGGATCGTTTTC+TGG	-	chr4.3:62716611-62716630	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	62716332	62716643	62716332	ID=MS.gene83729
chr4.3	mRNA	62716332	62716643	62716332	ID=MS.gene83729.t1;Parent=MS.gene83729
chr4.3	exon	62716332	62716389	62716332	ID=MS.gene83729.t1.exon1;Parent=MS.gene83729.t1
chr4.3	CDS	62716332	62716389	62716332	ID=cds.MS.gene83729.t1;Parent=MS.gene83729.t1
chr4.3	exon	62716471	62716643	62716471	ID=MS.gene83729.t1.exon2;Parent=MS.gene83729.t1
chr4.3	CDS	62716471	62716643	62716471	ID=cds.MS.gene83729.t1;Parent=MS.gene83729.t1

Gene Sequence	>MS.gene83729 ATGAGTTTCTCTACTAAAGCAACAAGGAACATTCTTGGTGCTCCTGACATGTATGCAAGTAAGTGAACATCTATAACAGCTCCCATTCCATTTGTGTGCTTGCGTTTTGCATATATAATTTGTTCTTTTATGGTTTCAGATGAAATCCATAAGAATAGAAAGCTACTTGCCCCAGATCAGCGATGTAAGAGAGGAAGAATGACTGTTGAACTTAATCAAACTCCCGTTGTACTTAATAAAACTGGTTCTGTATTTAATAAAACTCACGAGGAAAAGCCAGAAAACGATCCACGTTGCCACAATTATCTTTGA
Protein sequence	>MS.gene83729.t1 MSFSTKATRNILGAPDMYANEIHKNRKLLAPDQRCKRGRMTVELNQTPVVLNKTGSVFNKTHEEKPENDPRCHNYL