AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85127

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85127.t1 MTR_2g101620 96.937 555 17 0 1 555 1 555 0.0 1115
MS.gene85127.t1 MTR_2g009800 90.450 555 53 0 1 555 1 555 0.0 1043
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85127.t1 AT5G14050 61.837 566 185 10 1 555 1 546 0.0 670

Find 126 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGAAGATGCTTTGAATTT+TGG 0.136326 2.4:+3191163 None:intergenic
CTCTACTCCCCTCTTGAATT+TGG 0.174548 2.4:-3191821 MS.gene85127:CDS
ATGGAACTGAACCGAATTAA+TGG 0.250496 2.4:+3191262 None:intergenic
TGAAATCCACTTCAGTATTC+AGG 0.253259 2.4:+3190613 None:intergenic
TAGCCAAAGTTAACAGATTA+AGG 0.262384 2.4:-3191601 MS.gene85127:CDS
GACTGGGCACAGCTTGATTC+AGG 0.267674 2.4:-3191485 MS.gene85127:CDS
CTTAACGTCATCCTCTCTTT+TGG 0.282888 2.4:+3191985 None:intergenic
AAGGCTAGAGTTGATAAAAT+TGG 0.310494 2.4:-3191044 MS.gene85127:CDS
CCGAGAAGTCAGAATCTTCT+TGG 0.315985 2.4:+3191702 None:intergenic
TTAACAGATTAAGGAAGTTA+AGG 0.316539 2.4:-3191592 MS.gene85127:CDS
TCCCCTCTTGAATTTGGAAA+TGG 0.323398 2.4:-3191815 MS.gene85127:CDS
TACAATAGTGAGGAATTTCT+TGG 0.340204 2.4:-3190669 MS.gene85127:CDS
CCAAGAAGATTCTGACTTCT+CGG 0.345962 2.4:-3191702 MS.gene85127:CDS
CTTTGCGAAGAAAGCCGGTT+TGG 0.353761 2.4:-3191658 MS.gene85127:CDS
TTCTAATGGTCCCATTAATT+CGG 0.354772 2.4:-3191273 MS.gene85127:CDS
AGGTTATCAATGGTCTTGAT+AGG 0.358287 2.4:+3190633 None:intergenic
GGTCAGCTGCTTCTTGCTGC+TGG 0.365476 2.4:-3191233 MS.gene85127:CDS
ACTTGTAGATGCAAACATTC+AGG 0.369324 2.4:-3191300 MS.gene85127:CDS
TTTGAAAGAATCTAAGATTC+CGG 0.381602 2.4:+3191204 None:intergenic
GGGTCTCAAGTGATAATATC+TGG 0.385058 2.4:-3191101 MS.gene85127:CDS
CCCCTCTTGAATTTGGAAAT+GGG 0.395309 2.4:-3191814 MS.gene85127:CDS
TGGACTAAAATCAATACAAC+GGG 0.404884 2.4:+3190458 None:intergenic
CTTCCTTAATCTGTTAACTT+TGG 0.410710 2.4:+3191598 None:intergenic
TTTGCGAAGAAAGCCGGTTT+GGG 0.414670 2.4:-3191657 MS.gene85127:CDS
AGATGGAAAGGAAGAACATT+TGG 0.415307 2.4:-3191897 MS.gene85127:CDS
ATGGCTGTAGGAAACGCTTC+AGG 0.416776 2.4:-3190423 MS.gene85127:CDS
TTCGAAAGGGGCAATCTTCA+AGG 0.417875 2.4:+3191144 None:intergenic
GAGGACGAGAATGTGATATC+TGG 0.419714 2.4:-3191563 MS.gene85127:CDS
TTATAGTCTTGATTTAGTTA+AGG 0.420893 2.4:-3191063 MS.gene85127:CDS
ACAAAATCAAAGCAATTAGT+TGG 0.426886 2.4:-3190915 MS.gene85127:CDS
ACAATAGTGAGGAATTTCTT+GGG 0.430835 2.4:-3190668 MS.gene85127:CDS
CAAAAGAGAGGATGACGTTA+AGG 0.431319 2.4:-3191984 MS.gene85127:CDS
TGGAACTGAACCGAATTAAT+GGG 0.436701 2.4:+3191263 None:intergenic
GAATTAATGGGACCATTAGA+AGG 0.443632 2.4:+3191275 None:intergenic
TCGGTTCAGTTCCATCGAAA+TGG 0.445521 2.4:-3191254 MS.gene85127:CDS
TTCAGTATTCAGGTTATCAA+TGG 0.445525 2.4:+3190623 None:intergenic
AAGTAATGTTGTTGCGAGCC+GGG 0.448192 2.4:-3191417 MS.gene85127:CDS
AACATTCAGGATCCTTCTAA+TGG 0.448781 2.4:-3191287 MS.gene85127:CDS
TTCTAAAATGATAGCCTTTG+TGG 0.449352 2.4:-3190967 MS.gene85127:CDS
AAATATTAGCAATCTGTTCA+AGG 0.450551 2.4:-3190569 MS.gene85127:CDS
TCTTCCTCATCCACCCAAAC+CGG 0.450796 2.4:+3191644 None:intergenic
TTTGCAGCTGGTTCGGCTAG+TGG 0.463183 2.4:-3190705 MS.gene85127:CDS
CTTAGATTCTTTCAAATCGA+TGG 0.465769 2.4:-3191197 MS.gene85127:CDS
GTAGATGAAGGCTGTTTAAA+CGG 0.466326 2.4:-3190762 MS.gene85127:CDS
GCTGCAAAATGTGTCCCTAC+TGG 0.467974 2.4:+3190720 None:intergenic
CAGATTAAGGAAGTTAAGGA+AGG 0.470846 2.4:-3191588 MS.gene85127:CDS
ACTGACCGTTCTGCTGATAG+TGG 0.477341 2.4:-3191737 MS.gene85127:CDS
TGTTGCGAGCCGGGGTTATG+AGG 0.478638 2.4:-3191408 MS.gene85127:CDS
AAGCATCTTTCTTGCCCGAT+GGG 0.479466 2.4:-3191121 MS.gene85127:CDS
GATGATGAATTGACAGATGA+TGG 0.480610 2.4:-3191440 MS.gene85127:CDS
AGCTCAACCGAGGAACTGAC+TGG 0.481203 2.4:-3191502 MS.gene85127:CDS
GAAGTAATGTTGTTGCGAGC+CGG 0.483388 2.4:-3191418 MS.gene85127:CDS
GTGATGGCCATGTTTACCAT+TGG 0.483908 2.4:-3190818 MS.gene85127:CDS
AAATTGGTCCGCTGTTGGAT+AGG 0.484483 2.4:-3191028 MS.gene85127:CDS
GGAAATGGGGATAACGATGA+AGG 0.486981 2.4:-3191800 MS.gene85127:CDS
AATTGGTCCGCTGTTGGATA+GGG 0.491909 2.4:-3191027 MS.gene85127:CDS
CAGAGTATGTGTCAAGATTG+AGG 0.492768 2.4:-3191538 MS.gene85127:CDS
ACGGTCAGTGAAGAACAAAT+TGG 0.494840 2.4:+3191751 None:intergenic
TGATGGCCATGTTTACCATT+GGG 0.497623 2.4:-3190817 MS.gene85127:CDS
TCTTTCAAATCGATGGCAAA+CGG 0.500797 2.4:-3191190 MS.gene85127:CDS
AAGGAAGTTAAGGAAGGAAG+AGG 0.502686 2.4:-3191582 MS.gene85127:CDS
AAAGAGGAAGCTAGATGGAA+AGG 0.505950 2.4:-3191909 MS.gene85127:CDS
TGATAAAATTGGTCCGCTGT+TGG 0.506211 2.4:-3191033 MS.gene85127:CDS
GGAAGCCTTTGCAGCAGTCA+TGG 0.506331 2.4:+3191772 None:intergenic
AGAGTATGTGTCAAGATTGA+GGG 0.513752 2.4:-3191537 MS.gene85127:CDS
TGATGCTTTGCGAAGAAAGC+CGG 0.516338 2.4:-3191663 MS.gene85127:CDS
ATAGCCTTTGTGGGTGATGA+AGG 0.525756 2.4:-3190957 MS.gene85127:CDS
CCCTACTGGAGAAGTACAAA+GGG 0.526256 2.4:+3190734 None:intergenic
AGCAGCTCAAAATTGTTGCC+AGG 0.528190 2.4:-3191341 MS.gene85127:CDS
AAAGCCGGTTTGGGTGGATG+AGG 0.539602 2.4:-3191648 MS.gene85127:CDS
TTTCTAATCTACTTTAGGCA+TGG 0.540589 2.4:+3190367 None:intergenic
TCTCAGAACAAATGAAGACT+TGG 0.543934 2.4:-3191372 MS.gene85127:CDS
CCCTCTTGAATTTGGAAATG+GGG 0.544010 2.4:-3191813 MS.gene85127:CDS
GAGCCGGGGTTATGAGGATC+TGG 0.545404 2.4:-3191402 MS.gene85127:CDS
ACTGGGCACAGCTTGATTCA+GGG 0.545660 2.4:-3191484 MS.gene85127:CDS
GGAAGCCATGACTGCTGCAA+AGG 0.547123 2.4:-3191777 MS.gene85127:CDS
CCCCATTTCCAAATTCAAGA+GGG 0.548968 2.4:+3191813 None:intergenic
GTAAACATGGCCATCACCAC+CGG 0.549474 2.4:+3190824 None:intergenic
CAATACAACGGGGATAATGC+AGG 0.553209 2.4:+3190469 None:intergenic
TTCTTGCTGCTGGATTAGAC+CGG 0.554524 2.4:-3191223 MS.gene85127:CDS
TGTGAATATTTACAATAGTG+AGG 0.554925 2.4:-3190679 MS.gene85127:CDS
CAATAGTGAGGAATTTCTTG+GGG 0.554998 2.4:-3190667 MS.gene85127:CDS
AAAGCATCTTTCTTGCCCGA+TGG 0.557386 2.4:-3191122 MS.gene85127:CDS
GAAGATTCTGACTTCTCGGA+TGG 0.559324 2.4:-3191698 MS.gene85127:CDS
TTGTTGAGCACCGGTGGTGA+TGG 0.564654 2.4:-3190834 MS.gene85127:CDS
TCTAAAATGATAGCCTTTGT+GGG 0.566464 2.4:-3190966 MS.gene85127:CDS
CCCTTTGTACTTCTCCAGTA+GGG 0.569388 2.4:-3190734 MS.gene85127:CDS
GCGAAGAAAGCCGGTTTGGG+TGG 0.571477 2.4:-3191654 MS.gene85127:CDS
GCCCTTTGTACTTCTCCAGT+AGG 0.573251 2.4:-3190735 MS.gene85127:CDS
TGATAACCTGAATACTGAAG+TGG 0.580415 2.4:-3190619 MS.gene85127:CDS
GGACAAAAGTTGTTGAGCAC+CGG 0.580640 2.4:-3190843 MS.gene85127:CDS
ATTATCACTTGAGACCCATC+GGG 0.584332 2.4:+3191107 None:intergenic
TATTATCACTTGAGACCCAT+CGG 0.587218 2.4:+3191106 None:intergenic
GCTGTGCCCAGTCAGTTCCT+CGG 0.588930 2.4:+3191495 None:intergenic
GAAATGGGGATAACGATGAA+GGG 0.589801 2.4:-3191799 MS.gene85127:CDS
CTTCAGTAAAAGCTAACGCA+CGG 0.597212 2.4:+3190868 None:intergenic
AGTAATGTTGTTGCGAGCCG+GGG 0.597573 2.4:-3191416 MS.gene85127:CDS
GAAGCAGCTGACCATTTCGA+TGG 0.604108 2.4:+3191243 None:intergenic
TCCCCATTTCCAAATTCAAG+AGG 0.605102 2.4:+3191812 None:intergenic
TCAGATCCCAATGGTAAACA+TGG 0.606421 2.4:+3190811 None:intergenic
TTCGAGTATTCAATATGACC+TGG 0.611892 2.4:+3191323 None:intergenic
TTCAGTAAAAGCTAACGCAC+GGG 0.612384 2.4:+3190869 None:intergenic
TCCCTACTGGAGAAGTACAA+AGG 0.621550 2.4:+3190733 None:intergenic
CTGGACTAAAATCAATACAA+CGG 0.621707 2.4:+3190457 None:intergenic
ACCATTGGGATCTGAGAACA+AGG 0.624102 2.4:-3190803 MS.gene85127:CDS
CCCATTTCCAAATTCAAGAG+GGG 0.625050 2.4:+3191814 None:intergenic
TAGAAATGAGACCAAAAGAG+AGG 0.629102 2.4:-3191996 MS.gene85127:CDS
GCTCAACCGAGGAACTGACT+GGG 0.636934 2.4:-3191501 MS.gene85127:CDS
CCAGTTAGAGAAAACAGTGT+AGG 0.639938 2.4:+3190509 None:intergenic
GTTGGTACTCTGAAGATGAA+CGG 0.640176 2.4:-3190897 MS.gene85127:CDS
TTAGAGAAAACAGTGTAGGA+TGG 0.640295 2.4:+3190513 None:intergenic
CATCCAGATCCTCATAACCC+CGG 0.640892 2.4:+3191399 None:intergenic
TGCAGGCTCGCATTTGAACG+CGG 0.645712 2.4:+3190486 None:intergenic
ACAGCCATAAAACCACCACC+TGG 0.646807 2.4:+3190438 None:intergenic
ATAACCTTCATCACCCACAA+AGG 0.647464 2.4:+3190953 None:intergenic
AAATGGGGATAACGATGAAG+GGG 0.648646 2.4:-3191798 MS.gene85127:CDS
CAAAAGTTGTTGAGCACCGG+TGG 0.649375 2.4:-3190840 MS.gene85127:CDS
AATAGTGAGGAATTTCTTGG+GGG 0.659821 2.4:-3190666 MS.gene85127:CDS
ATGCACAAAGGTGTAGATGA+AGG 0.661644 2.4:-3190774 MS.gene85127:CDS
TTCTCTTCCCTATCCAACAG+CGG 0.664935 2.4:+3191020 None:intergenic
AATCTTCTTGGTAAACAGTG+AGG 0.688434 2.4:+3191714 None:intergenic
TGAGGCCACTATCAGCAGAA+CGG 0.695939 2.4:+3191732 None:intergenic
CAACATGTGAAGCTCAACCG+AGG 0.705882 2.4:-3191512 MS.gene85127:CDS
ACAAGGACTTGTATGCACAA+AGG 0.714733 2.4:-3190786 MS.gene85127:CDS
TCCTTGTTCTCAGATCCCAA+TGG 0.719029 2.4:+3190802 None:intergenic
GGACTAAAATCAATACAACG+GGG 0.796660 2.4:+3190459 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATAGTCTTGATTTAGTTA+AGG - chr2.4:3191342-3191361 MS.gene85127:CDS 20.0%
! AAATATTAGCAATCTGTTCA+AGG - chr2.4:3191836-3191855 MS.gene85127:CDS 25.0%
! ACAAAATCAAAGCAATTAGT+TGG - chr2.4:3191490-3191509 MS.gene85127:CDS 25.0%
! TGTGAATATTTACAATAGTG+AGG - chr2.4:3191726-3191745 MS.gene85127:CDS 25.0%
! TTAACAGATTAAGGAAGTTA+AGG - chr2.4:3190813-3190832 MS.gene85127:CDS 25.0%
!! AAACTTGAAAGCTTTTTGTT+TGG - chr2.4:3190557-3190576 MS.gene85127:CDS 25.0%
!! TGATGAAGGTTATATTTTAC+TGG - chr2.4:3191462-3191481 MS.gene85127:CDS 25.0%
!! TTTGAAAGAATCTAAGATTC+CGG + chr2.4:3191204-3191223 None:intergenic 25.0%
!!! AACTTGAAAGCTTTTTGTTT+GGG - chr2.4:3190558-3190577 MS.gene85127:CDS 25.0%
AAGGAAAAAGATGTTGAAGA+TGG - chr2.4:3190440-3190459 MS.gene85127:CDS 30.0%
ACAATAGTGAGGAATTTCTT+GGG - chr2.4:3191737-3191756 MS.gene85127:CDS 30.0%
CTGGACTAAAATCAATACAA+CGG + chr2.4:3191951-3191970 None:intergenic 30.0%
CTTAGATTCTTTCAAATCGA+TGG - chr2.4:3191208-3191227 MS.gene85127:CDS 30.0%
CTTCCTTAATCTGTTAACTT+TGG + chr2.4:3190810-3190829 None:intergenic 30.0%
TACAATAGTGAGGAATTTCT+TGG - chr2.4:3191736-3191755 MS.gene85127:CDS 30.0%
TAGCCAAAGTTAACAGATTA+AGG - chr2.4:3190804-3190823 MS.gene85127:CDS 30.0%
TGGACTAAAATCAATACAAC+GGG + chr2.4:3191950-3191969 None:intergenic 30.0%
TTCTAATGGTCCCATTAATT+CGG - chr2.4:3191132-3191151 MS.gene85127:CDS 30.0%
! AAGAAAGATGCTTTTCGAAA+GGG + chr2.4:3191277-3191296 None:intergenic 30.0%
! TCTAAAATGATAGCCTTTGT+GGG - chr2.4:3191439-3191458 MS.gene85127:CDS 30.0%
! TTCAGTATTCAGGTTATCAA+TGG + chr2.4:3191785-3191804 None:intergenic 30.0%
!! AAGGAAGATGCTTTGAATTT+TGG + chr2.4:3191245-3191264 None:intergenic 30.0%
!! AAGGCTAGAGTTGATAAAAT+TGG - chr2.4:3191361-3191380 MS.gene85127:CDS 30.0%
!! TTCTAAAATGATAGCCTTTG+TGG - chr2.4:3191438-3191457 MS.gene85127:CDS 30.0%
!!! AAGGCTATCATTTTAGAATC+AGG + chr2.4:3191436-3191455 None:intergenic 30.0%
!!! GAAGAGTATTGAAGTTTTTG+AGG - chr2.4:3191405-3191424 MS.gene85127:CDS 30.0%
AACATTCAGGATCCTTCTAA+TGG - chr2.4:3191118-3191137 MS.gene85127:CDS 35.0%
AATAGTGAGGAATTTCTTGG+GGG - chr2.4:3191739-3191758 MS.gene85127:CDS 35.0%
AATCTTCTTGGTAAACAGTG+AGG + chr2.4:3190694-3190713 None:intergenic 35.0%
ACTTGTAGATGCAAACATTC+AGG - chr2.4:3191105-3191124 MS.gene85127:CDS 35.0%
AGAGTATGTGTCAAGATTGA+GGG - chr2.4:3190868-3190887 MS.gene85127:CDS 35.0%
AGATGGAAAGGAAGAACATT+TGG - chr2.4:3190508-3190527 MS.gene85127:CDS 35.0%
ATACCGTAGAAGTGAAAAAG+AGG - chr2.4:3190480-3190499 MS.gene85127:CDS 35.0%
ATGGAACTGAACCGAATTAA+TGG + chr2.4:3191146-3191165 None:intergenic 35.0%
CAATAGTGAGGAATTTCTTG+GGG - chr2.4:3191738-3191757 MS.gene85127:CDS 35.0%
CAGATTAAGGAAGTTAAGGA+AGG - chr2.4:3190817-3190836 MS.gene85127:CDS 35.0%
GAATTAATGGGACCATTAGA+AGG + chr2.4:3191133-3191152 None:intergenic 35.0%
GATGATGAATTGACAGATGA+TGG - chr2.4:3190965-3190984 MS.gene85127:CDS 35.0%
GGACTAAAATCAATACAACG+GGG + chr2.4:3191949-3191968 None:intergenic 35.0%
GTAGATGAAGGCTGTTTAAA+CGG - chr2.4:3191643-3191662 MS.gene85127:CDS 35.0%
TAGAAATGAGACCAAAAGAG+AGG - chr2.4:3190409-3190428 MS.gene85127:CDS 35.0%
TATTATCACTTGAGACCCAT+CGG + chr2.4:3191302-3191321 None:intergenic 35.0%
TCTCAGAACAAATGAAGACT+TGG - chr2.4:3191033-3191052 MS.gene85127:CDS 35.0%
TCTTTCAAATCGATGGCAAA+CGG - chr2.4:3191215-3191234 MS.gene85127:CDS 35.0%
TGAAATCCACTTCAGTATTC+AGG + chr2.4:3191795-3191814 None:intergenic 35.0%
TGATAACCTGAATACTGAAG+TGG - chr2.4:3191786-3191805 MS.gene85127:CDS 35.0%
TGGAACTGAACCGAATTAAT+GGG + chr2.4:3191145-3191164 None:intergenic 35.0%
TTAGAGAAAACAGTGTAGGA+TGG + chr2.4:3191895-3191914 None:intergenic 35.0%
TTCGAGTATTCAATATGACC+TGG + chr2.4:3191085-3191104 None:intergenic 35.0%
! AGAAAGATGCTTTTCGAAAG+GGG + chr2.4:3191276-3191295 None:intergenic 35.0%
! AGGTTATCAATGGTCTTGAT+AGG + chr2.4:3191775-3191794 None:intergenic 35.0%
! CAAGAAAGATGCTTTTCGAA+AGG + chr2.4:3191278-3191297 None:intergenic 35.0%
!! CTTCCTCTTTTTCACTTCTA+CGG + chr2.4:3190486-3190505 None:intergenic 35.0%
!!! CGTTGTATTGATTTTAGTCC+AGG - chr2.4:3191949-3191968 MS.gene85127:CDS 35.0%
!!! TGTATTGATTTTAGTCCAGG+TGG - chr2.4:3191952-3191971 MS.gene85127:CDS 35.0%
AAAGAGGAAGCTAGATGGAA+AGG - chr2.4:3190496-3190515 MS.gene85127:CDS 40.0%
AAATGGGGATAACGATGAAG+GGG - chr2.4:3190607-3190626 MS.gene85127:CDS 40.0%
AAGGAAGTTAAGGAAGGAAG+AGG - chr2.4:3190823-3190842 MS.gene85127:CDS 40.0%
ACAAGGACTTGTATGCACAA+AGG - chr2.4:3191619-3191638 MS.gene85127:CDS 40.0%
ACGGTCAGTGAAGAACAAAT+TGG + chr2.4:3190657-3190676 None:intergenic 40.0%
ATAACCTTCATCACCCACAA+AGG + chr2.4:3191455-3191474 None:intergenic 40.0%
ATTATCACTTGAGACCCATC+GGG + chr2.4:3191301-3191320 None:intergenic 40.0%
CAAAAGAGAGGATGACGTTA+AGG - chr2.4:3190421-3190440 MS.gene85127:CDS 40.0%
CAGAGTATGTGTCAAGATTG+AGG - chr2.4:3190867-3190886 MS.gene85127:CDS 40.0%
CCAAGAAGATTCTGACTTCT+CGG - chr2.4:3190703-3190722 MS.gene85127:CDS 40.0%
CCAGTTAGAGAAAACAGTGT+AGG + chr2.4:3191899-3191918 None:intergenic 40.0%
CCCATTTCCAAATTCAAGAG+GGG + chr2.4:3190594-3190613 None:intergenic 40.0%
CCCCATTTCCAAATTCAAGA+GGG + chr2.4:3190595-3190614 None:intergenic 40.0%
CCCCTCTTGAATTTGGAAAT+GGG - chr2.4:3190591-3190610 MS.gene85127:CDS 40.0%
CCCTCTTGAATTTGGAAATG+GGG - chr2.4:3190592-3190611 MS.gene85127:CDS 40.0%
CTTCAGTAAAAGCTAACGCA+CGG + chr2.4:3191540-3191559 None:intergenic 40.0%
GAAATGGGGATAACGATGAA+GGG - chr2.4:3190606-3190625 MS.gene85127:CDS 40.0%
GATTCAGGGTCAAAAATGGA+TGG - chr2.4:3190935-3190954 MS.gene85127:CDS 40.0%
GCTTGATTCAGGGTCAAAAA+TGG - chr2.4:3190931-3190950 MS.gene85127:CDS 40.0%
GGGTCTCAAGTGATAATATC+TGG - chr2.4:3191304-3191323 MS.gene85127:CDS 40.0%
GTGAAAAAGAGGAAGCTAGA+TGG - chr2.4:3190491-3190510 MS.gene85127:CDS 40.0%
TCAGATCCCAATGGTAAACA+TGG + chr2.4:3191597-3191616 None:intergenic 40.0%
TCCCCATTTCCAAATTCAAG+AGG + chr2.4:3190596-3190615 None:intergenic 40.0%
TCCCCTCTTGAATTTGGAAA+TGG - chr2.4:3190590-3190609 MS.gene85127:CDS 40.0%
TGATAAAATTGGTCCGCTGT+TGG - chr2.4:3191372-3191391 MS.gene85127:CDS 40.0%
TGATGGCCATGTTTACCATT+GGG - chr2.4:3191588-3191607 MS.gene85127:CDS 40.0%
TTCAGTAAAAGCTAACGCAC+GGG + chr2.4:3191539-3191558 None:intergenic 40.0%
! ATGCACAAAGGTGTAGATGA+AGG - chr2.4:3191631-3191650 MS.gene85127:CDS 40.0%
! CCTACACTGTTTTCTCTAAC+TGG - chr2.4:3191896-3191915 MS.gene85127:CDS 40.0%
! CTTAACGTCATCCTCTCTTT+TGG + chr2.4:3190423-3190442 None:intergenic 40.0%
! GTTGGTACTCTGAAGATGAA+CGG - chr2.4:3191508-3191527 MS.gene85127:CDS 40.0%
!! ATTGATTTTAGTCCAGGTGG+TGG - chr2.4:3191955-3191974 MS.gene85127:CDS 40.0%
!!! GCGTTAGCTTTTACTGAAGA+TGG - chr2.4:3191541-3191560 MS.gene85127:CDS 40.0%
AAATTGGTCCGCTGTTGGAT+AGG - chr2.4:3191377-3191396 MS.gene85127:CDS 45.0%
AAGCATCTTTCTTGCCCGAT+GGG - chr2.4:3191284-3191303 MS.gene85127:CDS 45.0%
AAGTAATGTTGTTGCGAGCC+GGG - chr2.4:3190988-3191007 MS.gene85127:CDS 45.0%
AATTGGTCCGCTGTTGGATA+GGG - chr2.4:3191378-3191397 MS.gene85127:CDS 45.0%
ACCATTGGGATCTGAGAACA+AGG - chr2.4:3191602-3191621 MS.gene85127:CDS 45.0%
AGCAGCTCAAAATTGTTGCC+AGG - chr2.4:3191064-3191083 MS.gene85127:CDS 45.0%
ATAGCCTTTGTGGGTGATGA+AGG - chr2.4:3191448-3191467 MS.gene85127:CDS 45.0%
CAATACAACGGGGATAATGC+AGG + chr2.4:3191939-3191958 None:intergenic 45.0%
CCCTTTGTACTTCTCCAGTA+GGG - chr2.4:3191671-3191690 MS.gene85127:CDS 45.0%
CCGAGAAGTCAGAATCTTCT+TGG + chr2.4:3190706-3190725 None:intergenic 45.0%
CTCTACTCCCCTCTTGAATT+TGG - chr2.4:3190584-3190603 MS.gene85127:CDS 45.0%
GAAGATTCTGACTTCTCGGA+TGG - chr2.4:3190707-3190726 MS.gene85127:CDS 45.0%
GAAGTAATGTTGTTGCGAGC+CGG - chr2.4:3190987-3191006 MS.gene85127:CDS 45.0%
GAGGACGAGAATGTGATATC+TGG - chr2.4:3190842-3190861 MS.gene85127:CDS 45.0%
GGAAATGGGGATAACGATGA+AGG - chr2.4:3190605-3190624 MS.gene85127:CDS 45.0%
GGACAAAAGTTGTTGAGCAC+CGG - chr2.4:3191562-3191581 MS.gene85127:CDS 45.0%
GTGATGGCCATGTTTACCAT+TGG - chr2.4:3191587-3191606 MS.gene85127:CDS 45.0%
TCCTTGTTCTCAGATCCCAA+TGG + chr2.4:3191606-3191625 None:intergenic 45.0%
TCGGTTCAGTTCCATCGAAA+TGG - chr2.4:3191151-3191170 MS.gene85127:CDS 45.0%
TGATGCTTTGCGAAGAAAGC+CGG - chr2.4:3190742-3190761 MS.gene85127:CDS 45.0%
TTCGAAAGGGGCAATCTTCA+AGG + chr2.4:3191264-3191283 None:intergenic 45.0%
TTCTCTTCCCTATCCAACAG+CGG + chr2.4:3191388-3191407 None:intergenic 45.0%
! AAAGCATCTTTCTTGCCCGA+TGG - chr2.4:3191283-3191302 MS.gene85127:CDS 45.0%
! CCCTACTGGAGAAGTACAAA+GGG + chr2.4:3191674-3191693 None:intergenic 45.0%
! GACACATTTTGCAGCTGGTT+CGG - chr2.4:3191693-3191712 MS.gene85127:CDS 45.0%
! TCCCTACTGGAGAAGTACAA+AGG + chr2.4:3191675-3191694 None:intergenic 45.0%
! TTCTTGCTGCTGGATTAGAC+CGG - chr2.4:3191182-3191201 MS.gene85127:CDS 45.0%
! TTTGCGAAGAAAGCCGGTTT+GGG - chr2.4:3190748-3190767 MS.gene85127:CDS 45.0%
!! TAGTCCAGGTGGTGGTTTTA+TGG - chr2.4:3191963-3191982 MS.gene85127:CDS 45.0%
ACAGCCATAAAACCACCACC+TGG + chr2.4:3191970-3191989 None:intergenic 50.0%
ACTGACCGTTCTGCTGATAG+TGG - chr2.4:3190668-3190687 MS.gene85127:CDS 50.0%
ACTGGGCACAGCTTGATTCA+GGG - chr2.4:3190921-3190940 MS.gene85127:CDS 50.0%
AGTAATGTTGTTGCGAGCCG+GGG - chr2.4:3190989-3191008 MS.gene85127:CDS 50.0%
ATGGCTGTAGGAAACGCTTC+AGG - chr2.4:3191982-3192001 MS.gene85127:CDS 50.0%
CAACATGTGAAGCTCAACCG+AGG - chr2.4:3190893-3190912 MS.gene85127:CDS 50.0%
CATCCAGATCCTCATAACCC+CGG + chr2.4:3191009-3191028 None:intergenic 50.0%
CTTTGCGAAGAAAGCCGGTT+TGG - chr2.4:3190747-3190766 MS.gene85127:CDS 50.0%
GAAGCAGCTGACCATTTCGA+TGG + chr2.4:3191165-3191184 None:intergenic 50.0%
GCCCTTTGTACTTCTCCAGT+AGG - chr2.4:3191670-3191689 MS.gene85127:CDS 50.0%
GCTGCAAAATGTGTCCCTAC+TGG + chr2.4:3191688-3191707 None:intergenic 50.0%
GTAAACATGGCCATCACCAC+CGG + chr2.4:3191584-3191603 None:intergenic 50.0%
TCTTCCTCATCCACCCAAAC+CGG + chr2.4:3190764-3190783 None:intergenic 50.0%
TGAGGCCACTATCAGCAGAA+CGG + chr2.4:3190676-3190695 None:intergenic 50.0%
! GTAGGGACACATTTTGCAGC+TGG - chr2.4:3191688-3191707 MS.gene85127:CDS 50.0%
!! CAAAAGTTGTTGAGCACCGG+TGG - chr2.4:3191565-3191584 MS.gene85127:CDS 50.0%
!!! GGTGGTGGTTTTATGGCTGT+AGG - chr2.4:3191970-3191989 MS.gene85127:CDS 50.0%
AGCTCAACCGAGGAACTGAC+TGG - chr2.4:3190903-3190922 MS.gene85127:CDS 55.0%
GACTGGGCACAGCTTGATTC+AGG - chr2.4:3190920-3190939 MS.gene85127:CDS 55.0%
GCTCAACCGAGGAACTGACT+GGG - chr2.4:3190904-3190923 MS.gene85127:CDS 55.0%
GGAAGCCATGACTGCTGCAA+AGG - chr2.4:3190628-3190647 MS.gene85127:CDS 55.0%
GGAAGCCTTTGCAGCAGTCA+TGG + chr2.4:3190636-3190655 None:intergenic 55.0%
TGCAGGCTCGCATTTGAACG+CGG + chr2.4:3191922-3191941 None:intergenic 55.0%
!! AAAGCCGGTTTGGGTGGATG+AGG - chr2.4:3190757-3190776 MS.gene85127:CDS 55.0%
!! TTGTTGAGCACCGGTGGTGA+TGG - chr2.4:3191571-3191590 MS.gene85127:CDS 55.0%
!! TTTGCAGCTGGTTCGGCTAG+TGG - chr2.4:3191700-3191719 MS.gene85127:CDS 55.0%
GAGCCGGGGTTATGAGGATC+TGG - chr2.4:3191003-3191022 MS.gene85127:CDS 60.0%
GCTGTGCCCAGTCAGTTCCT+CGG + chr2.4:3190913-3190932 None:intergenic 60.0%
GGTCAGCTGCTTCTTGCTGC+TGG - chr2.4:3191172-3191191 MS.gene85127:CDS 60.0%
TGTTGCGAGCCGGGGTTATG+AGG - chr2.4:3190997-3191016 MS.gene85127:CDS 60.0%
!! GCGAAGAAAGCCGGTTTGGG+TGG - chr2.4:3190751-3190770 MS.gene85127:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 3190380 3192047 3190380 ID=MS.gene85127
chr2.4 mRNA 3190380 3192047 3190380 ID=MS.gene85127.t1;Parent=MS.gene85127
chr2.4 exon 3190380 3192047 3190380 ID=MS.gene85127.t1.exon1;Parent=MS.gene85127.t1
chr2.4 CDS 3190380 3192047 3190380 ID=cds.MS.gene85127.t1;Parent=MS.gene85127.t1
Gene Sequence

>MS.gene85127

ATGAGTTTAATATCTCAAAATGCTCGCTCTAGAAATGAGACCAAAAGAGAGGATGACGTTAAGGAAAAAGATGTTGAAGATGGTCAAGATAATGATGTTGATACCGTAGAAGTGAAAAAGAGGAAGCTAGATGGAAAGGAAGAACATTTGGCAAAAGAACAAGTTAAAGAGATGAAAAAACTTGAAAGCTTTTTGTTTGGGTCTCTCTACTCCCCTCTTGAATTTGGAAATGGGGATAACGATGAAGGGGAAGCCATGACTGCTGCAAAGGCTTCCAATTTGTTCTTCACTGACCGTTCTGCTGATAGTGGCCTCACTGTTTACCAAGAAGATTCTGACTTCTCGGATGGAAGTTGTGATGATGATGCTTTGCGAAGAAAGCCGGTTTGGGTGGATGAGGAAGAAGAGAAAGTCACTGTTAACATAGCCAAAGTTAACAGATTAAGGAAGTTAAGGAAGGAAGAGGACGAGAATGTGATATCTGGTTCAGAGTATGTGTCAAGATTGAGGGCTCAACATGTGAAGCTCAACCGAGGAACTGACTGGGCACAGCTTGATTCAGGGTCAAAAATGGATGGATCTTCTGATGATGAATTGACAGATGATGGAAGTAATGTTGTTGCGAGCCGGGGTTATGAGGATCTGGATGATATTCTCAGAACAAATGAAGACTTGGTTGTGAAGAGCAGCTCAAAATTGTTGCCAGGTCATATTGAATACTCGAAACTTGTAGATGCAAACATTCAGGATCCTTCTAATGGTCCCATTAATTCGGTTCAGTTCCATCGAAATGGTCAGCTGCTTCTTGCTGCTGGATTAGACCGGAATCTTAGATTCTTTCAAATCGATGGCAAACGGAACACCAAAATTCAAAGCATCTTCCTTGAAGATTGCCCCTTTCGAAAAGCATCTTTCTTGCCCGATGGGTCTCAAGTGATAATATCTGGAAGAAGAAAGTTCTTTTATAGTCTTGATTTAGTTAAGGCTAGAGTTGATAAAATTGGTCCGCTGTTGGATAGGGAAGAGAAGAGTATTGAAGTTTTTGAGGTCTCTCCTGATTCTAAAATGATAGCCTTTGTGGGTGATGAAGGTTATATTTTACTGGTTTCAACAAAATCAAAGCAATTAGTTGGTACTCTGAAGATGAACGGAACAGCCCGTGCGTTAGCTTTTACTGAAGATGGACAAAAGTTGTTGAGCACCGGTGGTGATGGCCATGTTTACCATTGGGATCTGAGAACAAGGACTTGTATGCACAAAGGTGTAGATGAAGGCTGTTTAAACGGCACAGCCCTTTGTACTTCTCCAGTAGGGACACATTTTGCAGCTGGTTCGGCTAGTGGAGTTGTGAATATTTACAATAGTGAGGAATTTCTTGGGGGAAAGAGAAAACCTATCAAGACCATTGATAACCTGAATACTGAAGTGGATTTCATGAAATTTAATCATGACTCTCAAATATTAGCAATCTGTTCAAGGTCAAAGAATAGTAGTTTGAAATTAATACATATACCATCCTACACTGTTTTCTCTAACTGGCCGCGTTCAAATGCGAGCCTGCATTATCCCCGTTGTATTGATTTTAGTCCAGGTGGTGGTTTTATGGCTGTAGGAAACGCTTCAGGCAAAGTGTTACTATATAAGTTGCATCATTATCACCATGCCTAA
Protein sequence

>MS.gene85127.t1

MSLISQNARSRNETKREDDVKEKDVEDGQDNDVDTVEVKKRKLDGKEEHLAKEQVKEMKKLESFLFGSLYSPLEFGNGDNDEGEAMTAAKASNLFFTDRSADSGLTVYQEDSDFSDGSCDDDALRRKPVWVDEEEEKVTVNIAKVNRLRKLRKEEDENVISGSEYVSRLRAQHVKLNRGTDWAQLDSGSKMDGSSDDELTDDGSNVVASRGYEDLDDILRTNEDLVVKSSSKLLPGHIEYSKLVDANIQDPSNGPINSVQFHRNGQLLLAAGLDRNLRFFQIDGKRNTKIQSIFLEDCPFRKASFLPDGSQVIISGRRKFFYSLDLVKARVDKIGPLLDREEKSIEVFEVSPDSKMIAFVGDEGYILLVSTKSKQLVGTLKMNGTARALAFTEDGQKLLSTGGDGHVYHWDLRTRTCMHKGVDEGCLNGTALCTSPVGTHFAAGSASGVVNIYNSEEFLGGKRKPIKTIDNLNTEVDFMKFNHDSQILAICSRSKNSSLKLIHIPSYTVFSNWPRSNASLHYPRCIDFSPGGGFMAVGNASGKVLLYKLHHYHHA