AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86036


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86036.t1 MTR_6g012830 95.385 195 9 0 2 196 43 237 2.68e-141 393
MS.gene86036.t1 MTR_6g012830 95.062 162 8 0 2 163 43 204 2.51e-115 327
MS.gene86036.t1 MTR_6g012830 82.051 195 7 1 2 196 43 209 8.47e-114 323
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86036.t1 AT5G39530 46.739 184 97 1 4 187 50 232 1.88e-59 186
MS.gene86036.t1 AT5G39520 42.246 187 106 2 7 193 45 229 2.68e-53 170
MS.gene86036.t1 AT5G39530 44.944 178 89 2 4 181 50 218 2.01e-52 167

Find 35 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACTCTTTCCCTTCCTTTC+TGG 0.048858 6.3:-8136204 None:intergenic
ACCCCTGGTAACTCATAAAA+AGG 0.262948 6.3:-8135106 None:intergenic
CTTAACCTTACATCTACTTT+TGG 0.275287 6.3:-8135819 None:intergenic
GGCTTCCTTTGATCAATATA+TGG 0.278362 6.3:+8135219 MS.gene86036:intron
AAAGCAGTGTTTCCTGAAAA+AGG 0.293804 6.3:+8135262 MS.gene86036:CDS
TATACAATGCTTCATCTTGA+AGG 0.378322 6.3:+8135791 MS.gene86036:CDS
TGTATAAGGTATTGAGTCCT+TGG 0.417418 6.3:-8136137 None:intergenic
GACTTCAACTTAGATGTAAG+AGG 0.434676 6.3:+8136171 MS.gene86036:CDS
AACACTGCTTTGACTACTCT+TGG 0.446906 6.3:-8135250 None:intergenic
AGGTTAAGAATAAAGTCTAA+TGG 0.464292 6.3:+8135834 MS.gene86036:CDS
TTCAACCAAAAGTAGATGTA+AGG 0.465816 6.3:+8135814 MS.gene86036:CDS
GTCGTAAGGATCATTGTATA+AGG 0.478912 6.3:-8136151 None:intergenic
CAAATGAAGCAGGAATTTCA+TGG 0.480806 6.3:+8136612 MS.gene86036:CDS
AAAGGAAGGGAAAGAGTAGT+TGG 0.497535 6.3:+8136208 MS.gene86036:CDS
TTCAAGATGAAGCATTGTAT+AGG 0.516620 6.3:-8135789 None:intergenic
TATTATATATCCAGAAAGGA+AGG 0.525308 6.3:+8136194 MS.gene86036:CDS
TCAGGAACAAAAGTCATTGT+TGG 0.526181 6.3:-8136273 None:intergenic
GCATCTCGTAAGATATGCTC+AGG 0.537298 6.3:-8136291 None:intergenic
CTTACGAGATGCATTAGAGT+TGG 0.542520 6.3:+8136302 MS.gene86036:CDS
ACATCTAAGTTGAAGTCGTA+AGG 0.548661 6.3:-8136165 None:intergenic
TTATATGTATACAGGAGGAA+TGG 0.554629 6.3:+8135751 MS.gene86036:intron
GATGTGGAATCAAATGAAGC+AGG 0.560904 6.3:+8136602 MS.gene86036:CDS
AATGGACCACATGTTTAGAC+TGG 0.571782 6.3:-8136687 None:intergenic
AAGATGAAGCATTGTATAGG+AGG 0.579892 6.3:-8135786 None:intergenic
TAGTTGGCTCAAGAATCAAA+TGG 0.580045 6.3:+8136224 MS.gene86036:CDS
ATCATCCATATATTGATCAA+AGG 0.580382 6.3:-8135224 None:intergenic
GAGGTATTATATATCCAGAA+AGG 0.582099 6.3:+8136190 MS.gene86036:CDS
ATGTGATGAGGAATGTGAGG+TGG 0.585549 6.3:-8135867 None:intergenic
CAGACGAGGTGGGAGCTCCA+AGG 0.602105 6.3:+8136120 MS.gene86036:intron
ATTATATATCCAGAAAGGAA+GGG 0.617916 6.3:+8136195 MS.gene86036:CDS
TTAAATCCTATCAGACGAGG+TGG 0.635432 6.3:+8136109 MS.gene86036:intron
AACAGGTATTCAAAACGATG+TGG 0.655966 6.3:+8136586 MS.gene86036:intron
AAACTCTAAACAGCTCAATG+AGG 0.660173 6.3:+8135285 MS.gene86036:CDS
TAAATCCTATCAGACGAGGT+GGG 0.681527 6.3:+8136110 MS.gene86036:intron
CTAATGTGATGAGGAATGTG+AGG 0.705321 6.3:-8135870 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCTTCAAAAAAAAAATA+TGG + chr6.3:8135541-8135560 MS.gene86036:intron 10.0%
!!! AAAAATATGGTTTTAAATTA+TGG + chr6.3:8135554-8135573 MS.gene86036:intron 10.0%
!!! TTAATTTTAAGGTAATATAA+AGG - chr6.3:8135592-8135611 None:intergenic 10.0%
!!! TTTTTTTCTTCTTAATTTTA+AGG - chr6.3:8135603-8135622 None:intergenic 10.0%
!! AAAATTTATGTTAGAATCAT+TGG - chr6.3:8135183-8135202 None:intergenic 15.0%
!! AAATTAAGAAGAAAAAAATG+TGG + chr6.3:8135605-8135624 MS.gene86036:intron 15.0%
!! ATATACAAATATAAGCTATT+TGG - chr6.3:8136514-8136533 None:intergenic 15.0%
!! ATATTTCATCAATCATATTA+TGG + chr6.3:8134934-8134953 MS.gene86036:intron 15.0%
!!! TATAATTTGTTGAAGTAATA+TGG + chr6.3:8135513-8135532 MS.gene86036:intron 15.0%
!!! TATATGTTGATAAAATTAAC+AGG + chr6.3:8136569-8136588 MS.gene86036:intron 15.0%
!! AACAATTTAGAAGAGAAAAT+TGG - chr6.3:8134766-8134785 None:intergenic 20.0%
!! AACTTATCTAATTTGAACTA+AGG + chr6.3:8136385-8136404 MS.gene86036:intron 20.0%
!! ACTATTGTTTATATGTATAC+AGG + chr6.3:8135743-8135762 MS.gene86036:intron 20.0%
!! ACTTATCTAATTTGAACTAA+GGG + chr6.3:8136386-8136405 MS.gene86036:intron 20.0%
!!! GATCATTCTTATTTCATTTT+CGG + chr6.3:8135978-8135997 MS.gene86036:intron 20.0%
!!! TACTCATTAGCTTTAATTAA+GGG - chr6.3:8135714-8135733 None:intergenic 20.0%
!!! TATAACAATTTTAGAGGTTT+CGG - chr6.3:8135654-8135673 None:intergenic 20.0%
! AAATTGTTATATTGCGATTG+CGG + chr6.3:8135663-8135682 MS.gene86036:intron 25.0%
! AGGTTAAGAATAAAGTCTAA+TGG + chr6.3:8135834-8135853 MS.gene86036:CDS 25.0%
! ATCATCCATATATTGATCAA+AGG - chr6.3:8135227-8135246 None:intergenic 25.0%
! ATTATATATCCAGAAAGGAA+GGG + chr6.3:8136195-8136214 MS.gene86036:CDS 25.0%
! ATTGTTTATATGTATACAGG+AGG + chr6.3:8135746-8135765 MS.gene86036:intron 25.0%
! CTAAAAGTGACACAATATAA+AGG - chr6.3:8135474-8135493 None:intergenic 25.0%
! TATTATATATCCAGAAAGGA+AGG + chr6.3:8136194-8136213 MS.gene86036:CDS 25.0%
! TCACGAATATAATTGATTGA+CGG + chr6.3:8136056-8136075 MS.gene86036:intron 25.0%
! TTTAATTAAGGGACACAATA+TGG - chr6.3:8135703-8135722 None:intergenic 25.0%
!! AAAAGATGACATGTTTGTTA+CGG - chr6.3:8136486-8136505 None:intergenic 25.0%
!! ATTTTTGTGATTTTCTTTGC+AGG + chr6.3:8135198-8135217 MS.gene86036:intron 25.0%
!! CTTACTATATTAGACTTGTT+TGG + chr6.3:8136343-8136362 MS.gene86036:intron 25.0%
!! TATTAGTACAAAAGAAGCAT+AGG + chr6.3:8134987-8135006 MS.gene86036:intron 25.0%
!! TCGCAATATAACAATTTTAG+AGG - chr6.3:8135660-8135679 None:intergenic 25.0%
!! TGCTTCTTTTGTACTAATAA+TGG - chr6.3:8134986-8135005 None:intergenic 25.0%
!! TTATGTTAGAATCATTGGTT+TGG - chr6.3:8135178-8135197 None:intergenic 25.0%
!!! ACACAATATAAAGGTTTTAG+AGG - chr6.3:8135465-8135484 None:intergenic 25.0%
!!! GTACTCATTAGCTTTAATTA+AGG - chr6.3:8135715-8135734 None:intergenic 25.0%
AATACTTCTATAGCTGAATG+AGG - chr6.3:8135077-8135096 None:intergenic 30.0%
ACAATAGTTTAATTCTCGTC+CGG - chr6.3:8136440-8136459 None:intergenic 30.0%
AGAAAAAAATGTGGTCAATG+CGG + chr6.3:8135614-8135633 MS.gene86036:intron 30.0%
CTAATTTGAACTAAGGGATA+AGG + chr6.3:8136392-8136411 MS.gene86036:intron 30.0%
GAAGATGAAGATGGTAATTA+AGG - chr6.3:8135151-8135170 None:intergenic 30.0%
TATACAATGCTTCATCTTGA+AGG + chr6.3:8135791-8135810 MS.gene86036:CDS 30.0%
TATTATGGCAAGTATTAGTG+TGG + chr6.3:8134949-8134968 MS.gene86036:intron 30.0%
TTACGTTGCAAACCTTTATT+TGG + chr6.3:8135385-8135404 MS.gene86036:intron 30.0%
TTATATGTATACAGGAGGAA+TGG + chr6.3:8135751-8135770 MS.gene86036:intron 30.0%
TTCAACCAAAAGTAGATGTA+AGG + chr6.3:8135814-8135833 MS.gene86036:CDS 30.0%
! AAACTTTGATAATATACCCC+TGG - chr6.3:8135124-8135143 None:intergenic 30.0%
! AGAATTTTGCTAATGTGATG+AGG - chr6.3:8135882-8135901 None:intergenic 30.0%
! CATTTTGTAAGCACTTTCAT+TGG + chr6.3:8135909-8135928 MS.gene86036:intron 30.0%
! CTTAACCTTACATCTACTTT+TGG - chr6.3:8135822-8135841 None:intergenic 30.0%
! GAGGTATTATATATCCAGAA+AGG + chr6.3:8136190-8136209 MS.gene86036:CDS 30.0%
! TTCCTTTTTATGAGTTACCA+GGG + chr6.3:8135104-8135123 MS.gene86036:CDS 30.0%
!! TGTTTAGAGTTTCCTTTTTC+AGG - chr6.3:8135277-8135296 None:intergenic 30.0%
!! TTAGAATCATTGGTTTGGAT+TGG - chr6.3:8135173-8135192 None:intergenic 30.0%
!! TTCAAGATGAAGCATTGTAT+AGG - chr6.3:8135792-8135811 None:intergenic 30.0%
!!! CACTTTTAGAACTATGTGTA+TGG + chr6.3:8135485-8135504 MS.gene86036:intron 30.0%
AAACTCTAAACAGCTCAATG+AGG + chr6.3:8135285-8135304 MS.gene86036:CDS 35.0%
AAAGCAGTGTTTCCTGAAAA+AGG + chr6.3:8135262-8135281 MS.gene86036:CDS 35.0%
AACAGGTATTCAAAACGATG+TGG + chr6.3:8136586-8136605 MS.gene86036:intron 35.0%
CAAATGAAGCAGGAATTTCA+TGG + chr6.3:8136612-8136631 MS.gene86036:CDS 35.0%
CAATAGTTTAATTCTCGTCC+GGG - chr6.3:8136439-8136458 None:intergenic 35.0%
CAATTAAATCCTATCAGACG+AGG + chr6.3:8136106-8136125 MS.gene86036:intron 35.0%
CATCATGTTCATCAGTGATT+AGG + chr6.3:8135038-8135057 MS.gene86036:intron 35.0%
GACTTCAACTTAGATGTAAG+AGG + chr6.3:8136171-8136190 MS.gene86036:CDS 35.0%
GGCTTCCTTTGATCAATATA+TGG + chr6.3:8135219-8135238 MS.gene86036:intron 35.0%
GTCGTAAGGATCATTGTATA+AGG - chr6.3:8136154-8136173 None:intergenic 35.0%
TCAGGAACAAAAGTCATTGT+TGG - chr6.3:8136276-8136295 None:intergenic 35.0%
TCGGATAATATGCATCGTTA+GGG + chr6.3:8135997-8136016 MS.gene86036:intron 35.0%
TGTACTAATAATGGTTGCCA+AGG - chr6.3:8134977-8134996 None:intergenic 35.0%
TTCGGATAATATGCATCGTT+AGG + chr6.3:8135996-8136015 MS.gene86036:intron 35.0%
TTTCCTTCTCAACAAGACAA+AGG - chr6.3:8134892-8134911 None:intergenic 35.0%
! ACATCTAAGTTGAAGTCGTA+AGG - chr6.3:8136168-8136187 None:intergenic 35.0%
! GTTCCTTTTTATGAGTTACC+AGG + chr6.3:8135103-8135122 MS.gene86036:CDS 35.0%
! TAGTTGGCTCAAGAATCAAA+TGG + chr6.3:8136224-8136243 MS.gene86036:CDS 35.0%
! TCCTTTTTATGAGTTACCAG+GGG + chr6.3:8135105-8135124 MS.gene86036:CDS 35.0%
! TTTGGATTGGAAGATGAAGA+TGG - chr6.3:8135160-8135179 None:intergenic 35.0%
!! AAGATGAAGCATTGTATAGG+AGG - chr6.3:8135789-8135808 None:intergenic 35.0%
!! TGTATAAGGTATTGAGTCCT+TGG - chr6.3:8136140-8136159 None:intergenic 35.0%
AAAGGAAGGGAAAGAGTAGT+TGG + chr6.3:8136208-8136227 MS.gene86036:CDS 40.0%
AACACTGCTTTGACTACTCT+TGG - chr6.3:8135253-8135272 None:intergenic 40.0%
ACCCCTGGTAACTCATAAAA+AGG - chr6.3:8135109-8135128 None:intergenic 40.0%
ACTAAGGGATAAGGAGTTTG+AGG + chr6.3:8136401-8136420 MS.gene86036:intron 40.0%
CAAGTATTAGTGTGGTTCCT+TGG + chr6.3:8134957-8134976 MS.gene86036:intron 40.0%
CTAATGTGATGAGGAATGTG+AGG - chr6.3:8135873-8135892 None:intergenic 40.0%
CTTACGAGATGCATTAGAGT+TGG + chr6.3:8136302-8136321 MS.gene86036:CDS 40.0%
GATGTGGAATCAAATGAAGC+AGG + chr6.3:8136602-8136621 MS.gene86036:CDS 40.0%
TAAATCCTATCAGACGAGGT+GGG + chr6.3:8136110-8136129 MS.gene86036:intron 40.0%
TGCACATTACGGCCAAATAA+AGG - chr6.3:8135400-8135419 None:intergenic 40.0%
TGTTATATTGCGATTGCGGA+CGG + chr6.3:8135667-8135686 MS.gene86036:intron 40.0%
TTAAATCCTATCAGACGAGG+TGG + chr6.3:8136109-8136128 MS.gene86036:intron 40.0%
! GTACCTTTGTCTTGTTGAGA+AGG + chr6.3:8134886-8134905 MS.gene86036:intron 40.0%
AACTGCAATCACGGACTCAT+CGG - chr6.3:8135424-8135443 None:intergenic 45.0%
ATGTGATGAGGAATGTGAGG+TGG - chr6.3:8135870-8135889 None:intergenic 45.0%
CTACTCTTTCCCTTCCTTTC+TGG - chr6.3:8136207-8136226 None:intergenic 45.0%
GCATCTCGTAAGATATGCTC+AGG - chr6.3:8136294-8136313 None:intergenic 45.0%
TGGCATCACAACTGCAATCA+CGG - chr6.3:8135433-8135452 None:intergenic 45.0%
TTGCAGTTGTGATGCCATTG+TGG + chr6.3:8135436-8135455 MS.gene86036:intron 45.0%
! GACTCATCGGTTGCACATTA+CGG - chr6.3:8135411-8135430 None:intergenic 45.0%
! TTGAGGTCTGGTTTCAAACC+CGG + chr6.3:8136418-8136437 MS.gene86036:intron 45.0%
!!! GGTTTTAGAGGTCTCCACAA+TGG - chr6.3:8135453-8135472 None:intergenic 45.0%
! GGGATAAGGAGTTTGAGGTC+TGG + chr6.3:8136406-8136425 MS.gene86036:intron 50.0%
GAGCTCCCACCTCGTCTGAT+AGG - chr6.3:8136118-8136137 None:intergenic 60.0%
CAGACGAGGTGGGAGCTCCA+AGG + chr6.3:8136120-8136139 MS.gene86036:intron 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 8134756 8136695 8134756 ID=MS.gene86036
chr6.3 mRNA 8134756 8136695 8134756 ID=MS.gene86036.t1;Parent=MS.gene86036
chr6.3 exon 8134756 8134760 8134756 ID=MS.gene86036.t1.exon1;Parent=MS.gene86036.t1
chr6.3 CDS 8134756 8134760 8134756 ID=cds.MS.gene86036.t1;Parent=MS.gene86036.t1
chr6.3 exon 8135084 8135126 8135084 ID=MS.gene86036.t1.exon2;Parent=MS.gene86036.t1
chr6.3 CDS 8135084 8135126 8135084 ID=cds.MS.gene86036.t1;Parent=MS.gene86036.t1
chr6.3 exon 8135220 8135306 8135220 ID=MS.gene86036.t1.exon3;Parent=MS.gene86036.t1
chr6.3 CDS 8135220 8135306 8135220 ID=cds.MS.gene86036.t1;Parent=MS.gene86036.t1
chr6.3 exon 8135765 8135914 8135765 ID=MS.gene86036.t1.exon4;Parent=MS.gene86036.t1
chr6.3 CDS 8135765 8135914 8135765 ID=cds.MS.gene86036.t1;Parent=MS.gene86036.t1
chr6.3 exon 8136123 8136323 8136123 ID=MS.gene86036.t1.exon5;Parent=MS.gene86036.t1
chr6.3 CDS 8136123 8136323 8136123 ID=cds.MS.gene86036.t1;Parent=MS.gene86036.t1
chr6.3 exon 8136591 8136695 8136591 ID=MS.gene86036.t1.exon6;Parent=MS.gene86036.t1
chr6.3 CDS 8136591 8136695 8136591 ID=cds.MS.gene86036.t1;Parent=MS.gene86036.t1
Gene Sequence

>MS.gene86036

ATGTGGTCCAATTTTCTCTTCTAAATTGTTTTTTTTTTAAAACAATCTAATTTAATTAAATGATAAACATAAACATCACATGCATATAAATTAATATATCTTATCTTGTACTTTTTTTTTTGTTGCATAAGTACCTTTGTCTTGTTGAGAAGGAAAATCTTATAAAGCTAAATACTGCATATTTCATCAATCATATTATGGCAAGTATTAGTGTGGTTCCTTGGCAACCATTATTAGTACAAAAGAAGCATAGGCTTAATAGTCATTTTCATACAAAGAAAACATCATGTTCATCAGTGATTAGGAATGAAAAAACATCCTCATTCAGCTATAGAAGTATTGCTAATGTTCCTTTTTATGAGTTACCAGGGGTATATTATCAAAGTTTTCATCCTTAATTACCATCTTCATCTTCCAATCCAAACCAATGATTCTAACATAAATTTTTGTGATTTTCTTTGCAGGCTTCCTTTGATCAATATATGGATGATAGACCAAGAGTAGTCAAAGCAGTGTTTCCTGAAAAAGGAAACTCTAAACAGCTCAATGAGGTACTTTTTAGAGAAATATGTGTGTGATTGATTACGTTAAATTTGTTAAAGTAATATGATTTTCAATTTCGATCGTGTTTACGTTGCAAACCTTTATTTGGCCGTAATGTGCAACCGATGAGTCCGTGATTGCAGTTGTGATGCCATTGTGGAGACCTCTAAAACCTTTATATTGTGTCACTTTTAGAACTATGTGTATGGTTATCTATAATTTGTTGAAGTAATATGGTTTTAAATTCTTCAAAAAAAAAATATGGTTTTAAATTATGGTTACGCTGCGAACCTTTATATTACCTTAAAATTAAGAAGAAAAAAATGTGGTCAATGCGGAAGCAGTTATGATGCCGAAACCTCTAAAATTGTTATATTGCGATTGCGGACGGAAATTTAGAACCATATTGTGTCCCTTAATTAAAGCTAATGAGTACTATAAGAAACTATTGTTTATATGTATACAGGAGGAATGGAGAGTTGAAATGCCTCCTATACAATGCTTCATCTTGAAGGTTCAACCAAAAGTAGATGTAAGGTTAAGAATAAAGTCTAATGGAGAAGACTATCCACCTCACATTCCTCATCACATTAGCAAAATTCTTGAGCTTCATTTTGTAAGCACTTTCATTGGAACATATCTATAATTTTGTGACTTGTTTTTGTGAATTTCTTTATGTGATCATTCTTATTTCATTTTCGGATAATATGCATCGTTAGGGTAGACTAGCAAGCGATCTAAACTTTTTTTAATCATTTCACGAATATAATTGATTGACGGTGTAAGAAAATTACTGACGATGCATATCAATTAAATCCTATCAGACGAGGTGGGAGCTCCAAGGACTCAATACCTTATACAATGATCCTTACGACTTCAACTTAGATGTAAGAGGTATTATATATCCAGAAAGGAAGGGAAAGAGTAGTTGGCTCAAGAATCAAATGGAGATGAAGATAAACTTTACTGTTTCACCAACAATGACTTTTGTTCCTGAGCATATCTTACGAGATGCATTAGAGTTGGTATGATTTCTATTCATTTCTTACTATATTAGACTTGTTTGGATTGACTTATAATTATTTGAACTTATCTAATTTGAACTAAGGGATAAGGAGTTTGAGGTCTGGTTTCAAACCCGGACGAGAATTAAACTATTGTATTTCAAAATCATTTTTTATAATCCGTAACAAACATGTCATCTTTTTATATCAAAATAGCTTATATTTGTATATCCAAATAGCTTATATTTGTATATCAAATAGTGACATATATGTTGATAAAATTAACAGGTATTCAAAACGATGTGGAATCAAATGAAGCAGGAATTTCATGGTAATTTATTGACAGATTATAATAGTTTCAAGAGATACAAATCCAAGAAAAGTCCAGTCTAA

Protein sequence

>MS.gene86036.t1

MCYRSIANVPFYELPGASFDQYMDDRPRVVKAVFPEKGNSKQLNEEEWRVEMPPIQCFILKVQPKVDVRLRIKSNGEDYPPHIPHHISKILELHFTRWELQGLNTLYNDPYDFNLDVRGIIYPERKGKSSWLKNQMEMKINFTVSPTMTFVPEHILRDALELVFKTMWNQMKQEFHGNLLTDYNSFKRYKSKKSPV