Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87115.t1 | XP_013468904.1 | 100 | 360 | 0 | 0 | 1 | 360 | 69 | 428 | 1.80E-213 | 751.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87115.t1 | Q9S9K4 | 52.5 | 360 | 164 | 4 | 1 | 360 | 73 | 425 | 4.4e-112 | 406.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87115.t1 | A0A072VMV4 | 100.0 | 360 | 0 | 0 | 1 | 360 | 69 | 428 | 1.3e-213 | 751.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene87115 | 0.862551 | 4.68E-64 | -1.69E-46 |
| MS.gene049669 | MS.gene87115 | 0.806103 | 9.68E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene87115 | 0.830036 | 3.68E-55 | -1.69E-46 |
| MS.gene050227 | MS.gene87115 | 0.854833 | 9.46E-62 | -1.69E-46 |
| MS.gene050228 | MS.gene87115 | 0.852244 | 5.24E-61 | -1.69E-46 |
| MS.gene050330 | MS.gene87115 | -0.808513 | 2.98E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene87115 | 0.835643 | 1.49E-56 | -1.69E-46 |
| MS.gene050558 | MS.gene87115 | 0.80945 | 1.88E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene87115 | 0.848252 | 6.90E-60 | -1.69E-46 |
| MS.gene050721 | MS.gene87115 | 0.845373 | 4.23E-59 | -1.69E-46 |
| MS.gene050724 | MS.gene87115 | 0.826958 | 2.04E-54 | -1.69E-46 |
| MS.gene050780 | MS.gene87115 | 0.820327 | 7.27E-53 | -1.69E-46 |
| MS.gene050787 | MS.gene87115 | 0.809131 | 2.20E-50 | -1.69E-46 |
| MS.gene050820 | MS.gene87115 | 0.819444 | 1.16E-52 | -1.69E-46 |
| MS.gene050824 | MS.gene87115 | 0.803115 | 4.07E-49 | -1.69E-46 |
| MS.gene051265 | MS.gene87115 | 0.814461 | 1.52E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene87115 | 0.87969 | 1.01E-69 | -1.69E-46 |
| MS.gene052302 | MS.gene87115 | 0.800193 | 1.62E-48 | -1.69E-46 |
| MS.gene052376 | MS.gene87115 | 0.806552 | 7.78E-50 | -1.69E-46 |
| MS.gene052389 | MS.gene87115 | 0.861039 | 1.36E-63 | -1.69E-46 |
| MS.gene052586 | MS.gene87115 | 0.810701 | 1.01E-50 | -1.69E-46 |
| MS.gene053147 | MS.gene87115 | 0.80722 | 5.62E-50 | -1.69E-46 |
| MS.gene053811 | MS.gene87115 | 0.82829 | 9.76E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene87115 | 0.801158 | 1.03E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene87115 | 0.805995 | 1.02E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene87115 | 0.803535 | 3.33E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene87115 | 0.806332 | 8.66E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene87115 | 0.869812 | 2.34E-66 | -1.69E-46 |
| MS.gene054826 | MS.gene87115 | 0.844691 | 6.46E-59 | -1.69E-46 |
| MS.gene054930 | MS.gene87115 | 0.84666 | 1.89E-59 | -1.69E-46 |
| MS.gene054942 | MS.gene87115 | 0.801215 | 1.00E-48 | -1.69E-46 |
| MS.gene056506 | MS.gene87115 | 0.815624 | 8.40E-52 | -1.69E-46 |
| MS.gene057026 | MS.gene87115 | 0.840714 | 7.36E-58 | -1.69E-46 |
| MS.gene057194 | MS.gene87115 | 0.800509 | 1.39E-48 | -1.69E-46 |
| MS.gene05761 | MS.gene87115 | 0.827211 | 1.77E-54 | -1.69E-46 |
| MS.gene057967 | MS.gene87115 | 0.807436 | 5.06E-50 | -1.69E-46 |
| MS.gene058139 | MS.gene87115 | 0.804312 | 2.29E-49 | -1.69E-46 |
| MS.gene058378 | MS.gene87115 | 0.807395 | 5.16E-50 | -1.69E-46 |
| MS.gene058739 | MS.gene87115 | 0.81354 | 2.43E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene87115 | 0.808901 | 2.47E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene87115 | -0.851965 | 6.29E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGGAGTTGTTCTGACTTT+TGG | 0.238222 | 1.1:+58654560 | None:intergenic |
| GATTAATGAACATACATATT+TGG | 0.242714 | 1.1:+58654533 | None:intergenic |
| GAAGCCCTTGATGGAATAAT+TGG | 0.245572 | 1.1:-58654710 | MS.gene87115:CDS |
| TGGACATAGTAGTCCTTCTT+TGG | 0.254367 | 1.1:+58655580 | None:intergenic |
| CCCTCGGATATATTTGATTC+TGG | 0.259620 | 1.1:-58652593 | MS.gene87115:CDS |
| TGTCCTTTCCATCCTTAGTT+TGG | 0.271260 | 1.1:+58650943 | None:intergenic |
| GATATATTTGATTCTGGAAA+TGG | 0.285563 | 1.1:-58652587 | MS.gene87115:CDS |
| TGACTATCTGTTCCAATATA+AGG | 0.288332 | 1.1:-58651417 | MS.gene87115:intron |
| CTTGATGGAATAATTGGTTT+TGG | 0.295754 | 1.1:-58654704 | MS.gene87115:CDS |
| CTTGTACGATCTTGAGAATA+TGG | 0.302586 | 1.1:-58647675 | MS.gene87115:CDS |
| TCTCCATAAGTTATGCTATA+GGG | 0.307360 | 1.1:+58655232 | None:intergenic |
| GTCCTTTCCATCCTTAGTTT+GGG | 0.310111 | 1.1:+58650944 | None:intergenic |
| ACATATGATGGTCCGATTCC+TGG | 0.332528 | 1.1:-58655277 | MS.gene87115:CDS |
| ATGCGAGCTAATTTCAATTC+AGG | 0.348914 | 1.1:+58652401 | None:intergenic |
| GGAAAGGACATGACACTTCT+TGG | 0.353144 | 1.1:-58650930 | MS.gene87115:CDS |
| AAGTTCATACCACCTTATAT+TGG | 0.354647 | 1.1:+58651405 | None:intergenic |
| CAAATACCTTTGGAATTAGT+TGG | 0.360512 | 1.1:+58652504 | None:intergenic |
| AATATTTGCCATAGGGGAAT+TGG | 0.388793 | 1.1:-58654588 | MS.gene87115:CDS |
| TATTCCATCAAGGGCTTCTT+CGG | 0.390964 | 1.1:+58654715 | None:intergenic |
| AATGCATACATACACCGATT+TGG | 0.391207 | 1.1:+58655491 | None:intergenic |
| TAGGCTGGTGTCCGATGTAT+TGG | 0.403880 | 1.1:-58650984 | MS.gene87115:CDS |
| TGCCCCTATAGCATAACTTA+TGG | 0.404144 | 1.1:-58655235 | MS.gene87115:CDS |
| AGGTTGTATTTCACTAAACT+TGG | 0.408327 | 1.1:-58655613 | MS.gene87115:CDS |
| TCAAAATGTAGTTTGACAAC+TGG | 0.411155 | 1.1:+58651470 | None:intergenic |
| GATCTTGAGAATATGGCTAT+TGG | 0.412629 | 1.1:-58647668 | MS.gene87115:CDS |
| TATTTCACTAAACTTGGGCT+TGG | 0.419254 | 1.1:-58655607 | MS.gene87115:CDS |
| TTGAAGAATATTGAAGTTGA+TGG | 0.419885 | 1.1:-58652632 | MS.gene87115:CDS |
| TCTGAAGTATGAGATCCCTT+TGG | 0.425665 | 1.1:+58655334 | None:intergenic |
| TCTTCCGAAGAAGCCCTTGA+TGG | 0.436173 | 1.1:-58654719 | MS.gene87115:CDS |
| CTGAAGTATGAGATCCCTTT+GGG | 0.444221 | 1.1:+58655335 | None:intergenic |
| ATCTCCATAAGTTATGCTAT+AGG | 0.446865 | 1.1:+58655231 | None:intergenic |
| ATAAGTTATGCTATAGGGGC+AGG | 0.450756 | 1.1:+58655237 | None:intergenic |
| CAACTACTGGATACTATGTC+AGG | 0.454687 | 1.1:-58655201 | MS.gene87115:CDS |
| ATATATTTGATTCTGGAAAT+GGG | 0.463580 | 1.1:-58652586 | MS.gene87115:CDS |
| TTGTAACCTTGCAGTGTTGA+TGG | 0.473520 | 1.1:-58651500 | MS.gene87115:intron |
| TACTATGTCCAAGTTGATAC+AGG | 0.477388 | 1.1:-58655568 | MS.gene87115:CDS |
| TCCGCCTTACAACCAGGAAT+CGG | 0.485297 | 1.1:+58655265 | None:intergenic |
| TGGTTCCACCAATTCCCCTA+TGG | 0.487442 | 1.1:+58654580 | None:intergenic |
| GCTGTACAATCTGGCACATT+GGG | 0.492450 | 1.1:-58654746 | MS.gene87115:CDS |
| CACTGCCTTGACAATATTCG+TGG | 0.504030 | 1.1:-58654617 | MS.gene87115:CDS |
| TGTCCTACGAAAAGCCAAAT+CGG | 0.504507 | 1.1:-58655505 | MS.gene87115:CDS |
| CAAAACCAATTATTCCATCA+AGG | 0.505616 | 1.1:+58654705 | None:intergenic |
| CGCTGTACAATCTGGCACAT+TGG | 0.507679 | 1.1:-58654747 | MS.gene87115:CDS |
| ATTTGCCATAGGGGAATTGG+TGG | 0.515298 | 1.1:-58654585 | MS.gene87115:CDS |
| TGGTCCGATTCCTGGTTGTA+AGG | 0.515945 | 1.1:-58655269 | MS.gene87115:CDS |
| TAATTTCAATTCAGGCTGAC+GGG | 0.516305 | 1.1:+58652409 | None:intergenic |
| TATAGCATAACTTATGGAGA+TGG | 0.523472 | 1.1:-58655229 | MS.gene87115:CDS |
| GGTTGTATTTCACTAAACTT+GGG | 0.524940 | 1.1:-58655612 | MS.gene87115:CDS |
| GGAGATGGAAGTGCAACTAC+TGG | 0.525159 | 1.1:-58655214 | MS.gene87115:CDS |
| AGTTGGTCATAGACTATGAC+AGG | 0.528228 | 1.1:+58652521 | None:intergenic |
| AAAACCAATTATTCCATCAA+GGG | 0.529475 | 1.1:+58654706 | None:intergenic |
| TATGGAGATTTCCGTTGATC+CGG | 0.531316 | 1.1:+58655161 | None:intergenic |
| AAGGGCACAGTGATTGATAG+TGG | 0.533492 | 1.1:-58652563 | MS.gene87115:CDS |
| GGTGGTGGAATATTTGCCAT+AGG | 0.534159 | 1.1:-58654596 | MS.gene87115:CDS |
| TGGGCTTGGCTCTCCAAAGA+AGG | 0.534533 | 1.1:-58655593 | MS.gene87115:CDS |
| GTGGTGGAATATTTGCCATA+GGG | 0.535995 | 1.1:-58654595 | MS.gene87115:CDS |
| TTTGATTCTGGAAATGGGAA+GGG | 0.536848 | 1.1:-58652581 | MS.gene87115:CDS |
| GAAAAGTGTGACCCAAACTA+AGG | 0.537843 | 1.1:-58650955 | MS.gene87115:CDS |
| CTCCATAAGTTATGCTATAG+GGG | 0.541427 | 1.1:+58655233 | None:intergenic |
| GATTGATAGTGGAACAACCT+TGG | 0.548951 | 1.1:-58652552 | MS.gene87115:CDS |
| CTAATTTCAATTCAGGCTGA+CGG | 0.548990 | 1.1:+58652408 | None:intergenic |
| ATTTGATTCTGGAAATGGGA+AGG | 0.551449 | 1.1:-58652582 | MS.gene87115:CDS |
| ACAGCTTGCTGCATCTCGAA+AGG | 0.556530 | 1.1:-58654657 | MS.gene87115:CDS |
| AGTGTGACCCAAACTAAGGA+TGG | 0.559654 | 1.1:-58650951 | MS.gene87115:CDS |
| GGTGTTTCCGCCTTACAACC+AGG | 0.561933 | 1.1:+58655259 | None:intergenic |
| TAACCTTGCAGTGTTGATGG+TGG | 0.562260 | 1.1:-58651497 | MS.gene87115:intron |
| ACATACATATTTGGTACCAA+TGG | 0.562501 | 1.1:+58654542 | None:intergenic |
| TATCACTTCCTGTATCAACT+TGG | 0.567282 | 1.1:+58655560 | None:intergenic |
| AGGTGTGGCGCTGTACAATC+TGG | 0.571318 | 1.1:-58654755 | MS.gene87115:CDS |
| ATGGCGTACTTTCCAGCATA+CGG | 0.571746 | 1.1:+58652356 | None:intergenic |
| TTGAGAATATGGCTATTGGA+TGG | 0.573054 | 1.1:-58647664 | MS.gene87115:CDS |
| AAAAGTCAGAACAACTCCAT+TGG | 0.586123 | 1.1:-58654558 | MS.gene87115:CDS |
| CTTGACAATATTCGTGGTGG+TGG | 0.588390 | 1.1:-58654611 | MS.gene87115:CDS |
| TTGACTCTCTATGACCCAAA+GGG | 0.594661 | 1.1:-58655349 | MS.gene87115:CDS |
| AGGAATCGGACCATCATATG+TGG | 0.599832 | 1.1:+58655279 | None:intergenic |
| CTGGTGTCCGATGTATTGGC+TGG | 0.602129 | 1.1:-58650980 | MS.gene87115:CDS |
| AAGGACATGACACTTCTTGG+AGG | 0.602130 | 1.1:-58650927 | MS.gene87115:intron |
| GACCCAAACTAAGGATGGAA+AGG | 0.607035 | 1.1:-58650946 | MS.gene87115:CDS |
| CCAGAATCAAATATATCCGA+GGG | 0.608496 | 1.1:+58652593 | None:intergenic |
| ATTGAAATTAGCTCGCATTG+AGG | 0.611001 | 1.1:-58652396 | MS.gene87115:CDS |
| TCCAGAATCAAATATATCCG+AGG | 0.613600 | 1.1:+58652592 | None:intergenic |
| TGATGTTCTACAGCTTCCCT+CGG | 0.625997 | 1.1:-58652609 | MS.gene87115:CDS |
| CTTGACTCTCTATGACCCAA+AGG | 0.636415 | 1.1:-58655350 | MS.gene87115:CDS |
| TAAGTTATGCTATAGGGGCA+GGG | 0.637715 | 1.1:+58655238 | None:intergenic |
| CTATGACCAACTAATTCCAA+AGG | 0.638550 | 1.1:-58652510 | MS.gene87115:intron |
| TCCGATTCCTGGTTGTAAGG+CGG | 0.644021 | 1.1:-58655266 | MS.gene87115:CDS |
| CACCACCACGAATATTGTCA+AGG | 0.649946 | 1.1:+58654612 | None:intergenic |
| AACTACTGGATACTATGTCA+GGG | 0.651961 | 1.1:-58655200 | MS.gene87115:CDS |
| TGGTGGAATATTTGCCATAG+GGG | 0.652757 | 1.1:-58654594 | MS.gene87115:CDS |
| TATGACAGGAAGATAAGCCA+AGG | 0.661057 | 1.1:+58652535 | None:intergenic |
| TACAGGAAGTGATATTCTGT+GGG | 0.670376 | 1.1:-58655551 | MS.gene87115:CDS |
| ATACAGGAAGTGATATTCTG+TGG | 0.684061 | 1.1:-58655552 | MS.gene87115:CDS |
| TTGTAGGGCACATTACAATG+TGG | 0.689913 | 1.1:-58652657 | MS.gene87115:intron |
| TATTGGAACAGATAGTCATG+AGG | 0.699625 | 1.1:+58651422 | None:intergenic |
| TGCCTTGACAATATTCGTGG+TGG | 0.718049 | 1.1:-58654614 | MS.gene87115:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 58647651 | 58655633 | 58647651 | ID=MS.gene87115 |
| chr1.1 | mRNA | 58647651 | 58655633 | 58647651 | ID=MS.gene87115.t1;Parent=MS.gene87115 |
| chr1.1 | exon | 58655504 | 58655633 | 58655504 | ID=MS.gene87115.t1.exon1;Parent=MS.gene87115.t1 |
| chr1.1 | CDS | 58655504 | 58655633 | 58655504 | ID=cds.MS.gene87115.t1;Parent=MS.gene87115.t1 |
| chr1.1 | exon | 58655130 | 58655377 | 58655130 | ID=MS.gene87115.t1.exon2;Parent=MS.gene87115.t1 |
| chr1.1 | CDS | 58655130 | 58655377 | 58655130 | ID=cds.MS.gene87115.t1;Parent=MS.gene87115.t1 |
| chr1.1 | exon | 58654548 | 58654775 | 58654548 | ID=MS.gene87115.t1.exon3;Parent=MS.gene87115.t1 |
| chr1.1 | CDS | 58654548 | 58654775 | 58654548 | ID=cds.MS.gene87115.t1;Parent=MS.gene87115.t1 |
| chr1.1 | exon | 58652511 | 58652673 | 58652511 | ID=MS.gene87115.t1.exon4;Parent=MS.gene87115.t1 |
| chr1.1 | CDS | 58652511 | 58652673 | 58652511 | ID=cds.MS.gene87115.t1;Parent=MS.gene87115.t1 |
| chr1.1 | exon | 58652365 | 58652438 | 58652365 | ID=MS.gene87115.t1.exon5;Parent=MS.gene87115.t1 |
| chr1.1 | CDS | 58652365 | 58652438 | 58652365 | ID=cds.MS.gene87115.t1;Parent=MS.gene87115.t1 |
| chr1.1 | exon | 58651418 | 58651508 | 58651418 | ID=MS.gene87115.t1.exon6;Parent=MS.gene87115.t1 |
| chr1.1 | CDS | 58651418 | 58651508 | 58651418 | ID=cds.MS.gene87115.t1;Parent=MS.gene87115.t1 |
| chr1.1 | exon | 58650928 | 58651003 | 58650928 | ID=MS.gene87115.t1.exon7;Parent=MS.gene87115.t1 |
| chr1.1 | CDS | 58650928 | 58651003 | 58650928 | ID=cds.MS.gene87115.t1;Parent=MS.gene87115.t1 |
| chr1.1 | exon | 58647651 | 58647722 | 58647651 | ID=MS.gene87115.t1.exon8;Parent=MS.gene87115.t1 |
| chr1.1 | CDS | 58647651 | 58647722 | 58647651 | ID=cds.MS.gene87115.t1;Parent=MS.gene87115.t1 |
| Gene Sequence |
| Protein sequence |
