Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87969.t1 | XP_003618966.1 | 97.8 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 2.40E-42 | 181 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87969.t1 | G7KPM7 | 97.8 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 1.7e-42 | 181.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene87969 | 0.800413 | 1.46E-48 | -1.69E-46 |
| MS.gene049283 | MS.gene87969 | 0.809837 | 1.55E-50 | -1.69E-46 |
| MS.gene049580 | MS.gene87969 | 0.819651 | 1.04E-52 | -1.69E-46 |
| MS.gene04998 | MS.gene87969 | 0.807349 | 5.28E-50 | -1.69E-46 |
| MS.gene050945 | MS.gene87969 | 0.803567 | 3.28E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene87969 | 0.830781 | 2.42E-55 | -1.69E-46 |
| MS.gene051902 | MS.gene87969 | 0.818304 | 2.10E-52 | -1.69E-46 |
| MS.gene052296 | MS.gene87969 | 0.846425 | 2.19E-59 | -1.69E-46 |
| MS.gene052533 | MS.gene87969 | 0.821415 | 4.09E-53 | -1.69E-46 |
| MS.gene052793 | MS.gene87969 | 0.810206 | 1.29E-50 | -1.69E-46 |
| MS.gene052818 | MS.gene87969 | -0.830764 | 2.44E-55 | -1.69E-46 |
| MS.gene053045 | MS.gene87969 | 0.843467 | 1.38E-58 | -1.69E-46 |
| MS.gene053298 | MS.gene87969 | 0.83348 | 5.21E-56 | -1.69E-46 |
| MS.gene053340 | MS.gene87969 | 0.817901 | 2.59E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene87969 | 0.818346 | 2.06E-52 | -1.69E-46 |
| MS.gene054222 | MS.gene87969 | 0.85652 | 3.04E-62 | -1.69E-46 |
| MS.gene055555 | MS.gene87969 | 0.810338 | 1.21E-50 | -1.69E-46 |
| MS.gene056677 | MS.gene87969 | 0.800927 | 1.15E-48 | -1.69E-46 |
| MS.gene057102 | MS.gene87969 | 0.848042 | 7.88E-60 | -1.69E-46 |
| MS.gene057576 | MS.gene87969 | 0.813199 | 2.89E-51 | -1.69E-46 |
| MS.gene057584 | MS.gene87969 | 0.821039 | 4.99E-53 | -1.69E-46 |
| MS.gene057588 | MS.gene87969 | 0.802651 | 5.07E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene87969 | 0.814011 | 1.91E-51 | -1.69E-46 |
| MS.gene058074 | MS.gene87969 | 0.835532 | 1.59E-56 | -1.69E-46 |
| MS.gene058159 | MS.gene87969 | 0.805298 | 1.43E-49 | -1.69E-46 |
| MS.gene058592 | MS.gene87969 | 0.800436 | 1.44E-48 | -1.69E-46 |
| MS.gene058635 | MS.gene87969 | 0.806472 | 8.09E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene87969 | 0.826906 | 2.10E-54 | -1.69E-46 |
| MS.gene05985 | MS.gene87969 | -0.821641 | 3.63E-53 | -1.69E-46 |
| MS.gene06018 | MS.gene87969 | 0.829304 | 5.55E-55 | -1.69E-46 |
| MS.gene061100 | MS.gene87969 | 0.814967 | 1.18E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87969.t1 | MTR_6g029390 | 97.778 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 9.28e-62 | 182 |
| MS.gene87969.t1 | MTR_5g098200 | 83.333 | 84 | 14 | 0 | 1 | 84 | 1 | 84 | 4.45e-48 | 147 |
| MS.gene87969.t1 | MTR_7g085610 | 61.798 | 89 | 31 | 2 | 1 | 88 | 1 | 87 | 8.80e-35 | 114 |
| MS.gene87969.t1 | MTR_5g098280 | 47.368 | 76 | 37 | 1 | 1 | 76 | 1 | 73 | 1.80e-20 | 77.8 |
| MS.gene87969.t1 | MTR_3g466420 | 46.341 | 82 | 38 | 1 | 1 | 76 | 1 | 82 | 1.46e-19 | 75.9 |
| MS.gene87969.t1 | MTR_5g075070 | 42.697 | 89 | 42 | 2 | 1 | 80 | 1 | 89 | 3.35e-19 | 75.1 |
| MS.gene87969.t1 | MTR_0398s0030 | 43.210 | 81 | 41 | 1 | 1 | 76 | 1 | 81 | 1.34e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87969.t1 | AT5G18150 | 62.821 | 78 | 21 | 2 | 1 | 78 | 1 | 70 | 8.32e-31 | 103 |
| MS.gene87969.t1 | AT5G14602 | 57.143 | 84 | 32 | 2 | 1 | 84 | 1 | 80 | 4.76e-30 | 102 |
| MS.gene87969.t1 | AT5G58375 | 44.048 | 84 | 42 | 2 | 1 | 81 | 1 | 82 | 1.03e-16 | 68.6 |
Find 19 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGATTCAAAATGGATTT+TGG | 0.097169 | 6.1:-11188994 | MS.gene87969:CDS |
| AGAGAGAGATGTGTCCTTTA+AGG | 0.173917 | 6.1:-11189676 | None:intergenic |
| ATTGAAATCCTCTAAGAATT+AGG | 0.190751 | 6.1:-11188903 | None:intergenic |
| ATTGAGTCCCTAATTCTTAG+AGG | 0.300143 | 6.1:+11188895 | None:intergenic |
| AACACCAAGATGAACCTTAA+AGG | 0.300317 | 6.1:+11189662 | None:intergenic |
| GGTGTTCTTCTCTGCGGTTC+TGG | 0.325709 | 6.1:-11189645 | MS.gene87969:CDS |
| ATCTTCACCAAAGATTGATT+TGG | 0.357200 | 6.1:-11189594 | MS.gene87969:CDS |
| TTGAAATCCTCTAAGAATTA+GGG | 0.358948 | 6.1:-11188902 | None:intergenic |
| TCACAAGCCAAATCAATCTT+TGG | 0.382003 | 6.1:+11189587 | None:intergenic |
| TTCTGGCAGGTTACTTTGCA+TGG | 0.389470 | 6.1:-11189628 | MS.gene87969:CDS |
| TGGCTAGTGGAAGGTATCTA+TGG | 0.397595 | 6.1:-11188958 | MS.gene87969:CDS |
| TTCTTCTCTGCGGTTCTGGC+AGG | 0.404540 | 6.1:-11189641 | MS.gene87969:CDS |
| GTGTCCTTTAAGGTTCATCT+TGG | 0.474313 | 6.1:-11189666 | MS.gene87969:CDS |
| GGTATCTATGGAGAAATTTG+AGG | 0.538445 | 6.1:-11188946 | MS.gene87969:CDS |
| GGCTTGTGATGATTCAATTG+AGG | 0.570775 | 6.1:-11189573 | MS.gene87969:CDS |
| AGGTTACTTTGCATGGAGAA+CGG | 0.580884 | 6.1:-11189621 | MS.gene87969:CDS |
| GTATTCGTTGACATGGCTAG+TGG | 0.585469 | 6.1:-11188971 | MS.gene87969:CDS |
| CATCTTGGTGTTCTTCTCTG+CGG | 0.597461 | 6.1:-11189651 | MS.gene87969:CDS |
| TCGTTGACATGGCTAGTGGA+AGG | 0.632760 | 6.1:-11188967 | MS.gene87969:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTATATAATTGATCT+TGG | - | chr6.1:11189089-11189108 | MS.gene87969:intron | 10.0% |
| !!! | TTATTTTTAATTTTTGTGTT+TGG | - | chr6.1:11189511-11189530 | MS.gene87969:intron | 10.0% |
| !! | AAAATTAAAATTCCAAGATT+TGG | + | chr6.1:11189172-11189191 | None:intergenic | 15.0% |
| !! | AATTAATAACTAATGTTGAT+TGG | - | chr6.1:11189234-11189253 | MS.gene87969:intron | 15.0% |
| !!! | ACATAACTTTAAAAAGATAA+AGG | + | chr6.1:11189117-11189136 | None:intergenic | 15.0% |
| !!! | ATTAATAACTAATGTTGATT+GGG | - | chr6.1:11189235-11189254 | MS.gene87969:intron | 15.0% |
| !!! | ATGATATGCTAATTGATTTT+GGG | - | chr6.1:11189360-11189379 | MS.gene87969:intron | 20.0% |
| !!! | ATGTTTTATTGTTAAGGTTT+GGG | - | chr6.1:11189268-11189287 | MS.gene87969:intron | 20.0% |
| !!! | TAAATCTTGGAATTTTCTTT+GGG | - | chr6.1:11189453-11189472 | MS.gene87969:intron | 20.0% |
| !!! | TAGATGATGTTTTATTGTTA+AGG | - | chr6.1:11189262-11189281 | MS.gene87969:intron | 20.0% |
| !!! | TGTTTTGGTTTTTTAATCAT+GGG | - | chr6.1:11189317-11189336 | MS.gene87969:intron | 20.0% |
| !!! | TTAAATCTTGGAATTTTCTT+TGG | - | chr6.1:11189452-11189471 | MS.gene87969:intron | 20.0% |
| ! | AGAAAAATCATCTTTCAAGA+AGG | - | chr6.1:11189023-11189042 | MS.gene87969:intron | 25.0% |
| ! | ATTCACCAAAATATGTTGAT+TGG | - | chr6.1:11189407-11189426 | MS.gene87969:intron | 25.0% |
| ! | TTAATCATGGGAATAATGAT+TGG | - | chr6.1:11189329-11189348 | MS.gene87969:intron | 25.0% |
| !! | AACTCATCAAAATGTTGATT+GGG | - | chr6.1:11189550-11189569 | MS.gene87969:CDS | 25.0% |
| !! | TAAACCCAATCAACATATTT+TGG | + | chr6.1:11189415-11189434 | None:intergenic | 25.0% |
| !! | TTCACCAAAATATGTTGATT+GGG | - | chr6.1:11189408-11189427 | MS.gene87969:intron | 25.0% |
| !!! | AGATGATTCAAAATGGATTT+TGG | - | chr6.1:11189578-11189597 | MS.gene87969:CDS | 25.0% |
| !!! | GATGATATGCTAATTGATTT+TGG | - | chr6.1:11189359-11189378 | MS.gene87969:intron | 25.0% |
| !!! | GATGATTCAAAATGGATTTT+GGG | - | chr6.1:11189579-11189598 | MS.gene87969:CDS | 25.0% |
| !!! | GATGTTTTATTGTTAAGGTT+TGG | - | chr6.1:11189267-11189286 | MS.gene87969:intron | 25.0% |
| !!! | GGAAGATTTTGATTTCAAAA+AGG | - | chr6.1:11189044-11189063 | MS.gene87969:intron | 25.0% |
| !!! | GTGTTTTGGTTTTTTAATCA+TGG | - | chr6.1:11189316-11189335 | MS.gene87969:intron | 25.0% |
| ATCTTCACCAAAGATTGATT+TGG | - | chr6.1:11188978-11188997 | MS.gene87969:CDS | 30.0% | |
| GAACTCATCAAAATGTTGAT+TGG | - | chr6.1:11189549-11189568 | MS.gene87969:CDS | 30.0% | |
| ! | CTTTGTGTTTGATTGTGTTT+TGG | - | chr6.1:11189302-11189321 | MS.gene87969:intron | 30.0% |
| ! | GAGGTTTTCATGTTAAATCT+TGG | - | chr6.1:11189440-11189459 | MS.gene87969:intron | 30.0% |
| ! | GGTTTTCAGATGATTCAAAA+TGG | - | chr6.1:11189571-11189590 | MS.gene87969:CDS | 30.0% |
| AACACCAAGATGAACCTTAA+AGG | + | chr6.1:11188913-11188932 | None:intergenic | 35.0% | |
| GGTATCTATGGAGAAATTTG+AGG | - | chr6.1:11189626-11189645 | MS.gene87969:CDS | 35.0% | |
| GTTGATTGGGTTTATAGATG+AGG | - | chr6.1:11189421-11189440 | MS.gene87969:intron | 35.0% | |
| TAGTCTACGAATCCAAATCT+TGG | - | chr6.1:11189157-11189176 | MS.gene87969:intron | 35.0% | |
| TCACAAGCCAAATCAATCTT+TGG | + | chr6.1:11188988-11189007 | None:intergenic | 35.0% | |
| !! | ATTTTGGGTATTCGTTGACA+TGG | - | chr6.1:11189594-11189613 | MS.gene87969:CDS | 35.0% |
| !! | CTTGGAATTTTCTTTGGGTT+TGG | - | chr6.1:11189458-11189477 | MS.gene87969:intron | 35.0% |
| GGCTTGTGATGATTCAATTG+AGG | - | chr6.1:11188999-11189018 | MS.gene87969:intron | 40.0% | |
| ! | AGGTTACTTTGCATGGAGAA+CGG | - | chr6.1:11188951-11188970 | MS.gene87969:CDS | 40.0% |
| !! | GTGTCCTTTAAGGTTCATCT+TGG | - | chr6.1:11188906-11188925 | MS.gene87969:CDS | 40.0% |
| GTATTCGTTGACATGGCTAG+TGG | - | chr6.1:11189601-11189620 | MS.gene87969:CDS | 45.0% | |
| ! | TTCTGGCAGGTTACTTTGCA+TGG | - | chr6.1:11188944-11188963 | MS.gene87969:CDS | 45.0% |
| !! | CATCTTGGTGTTCTTCTCTG+CGG | - | chr6.1:11188921-11188940 | MS.gene87969:CDS | 45.0% |
| !! | TGGCTAGTGGAAGGTATCTA+TGG | - | chr6.1:11189614-11189633 | MS.gene87969:CDS | 45.0% |
| !!! | AAAAATAATAATTTTTTTGA+AGG | + | chr6.1:11189075-11189094 | None:intergenic | 5.0% |
| !!! | AAAATAATAATTTTTTTGAA+GGG | + | chr6.1:11189074-11189093 | None:intergenic | 5.0% |
| TCGTTGACATGGCTAGTGGA+AGG | - | chr6.1:11189605-11189624 | MS.gene87969:CDS | 50.0% | |
| GGTGTTCTTCTCTGCGGTTC+TGG | - | chr6.1:11188927-11188946 | MS.gene87969:CDS | 55.0% | |
| ! | TTCTTCTCTGCGGTTCTGGC+AGG | - | chr6.1:11188931-11188950 | MS.gene87969:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 11188904 | 11189690 | 11188904 | ID=MS.gene87969 |
| chr6.1 | mRNA | 11188904 | 11189690 | 11188904 | ID=MS.gene87969.t1;Parent=MS.gene87969 |
| chr6.1 | exon | 11189529 | 11189690 | 11189529 | ID=MS.gene87969.t1.exon1;Parent=MS.gene87969.t1 |
| chr6.1 | CDS | 11189529 | 11189690 | 11189529 | ID=cds.MS.gene87969.t1;Parent=MS.gene87969.t1 |
| chr6.1 | exon | 11188904 | 11189014 | 11188904 | ID=MS.gene87969.t1.exon2;Parent=MS.gene87969.t1 |
| chr6.1 | CDS | 11188904 | 11189014 | 11188904 | ID=cds.MS.gene87969.t1;Parent=MS.gene87969.t1 |
| Gene Sequence |
| Protein sequence |
