Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96799.t1 | XP_024638446.1 | 97.4 | 114 | 3 | 0 | 1 | 114 | 1 | 114 | 2.90E-53 | 217.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96799.t1 | A0A072UP09 | 97.4 | 114 | 3 | 0 | 1 | 114 | 1 | 114 | 2.1e-53 | 217.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene96799 | 0.800451 | 1.43E-48 | -1.69E-46 |
| MS.gene049378 | MS.gene96799 | 0.805776 | 1.13E-49 | -1.69E-46 |
| MS.gene049424 | MS.gene96799 | 0.822781 | 1.97E-53 | -1.69E-46 |
| MS.gene050226 | MS.gene96799 | 0.819689 | 1.02E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene96799 | 0.814402 | 1.57E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene96799 | 0.814121 | 1.81E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene96799 | 0.800481 | 1.41E-48 | -1.69E-46 |
| MS.gene050987 | MS.gene96799 | 0.83349 | 5.18E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene96799 | 0.80981 | 1.57E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene96799 | 0.805818 | 1.11E-49 | -1.69E-46 |
| MS.gene052286 | MS.gene96799 | 0.809473 | 1.86E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene96799 | 0.810526 | 1.10E-50 | -1.69E-46 |
| MS.gene053188 | MS.gene96799 | 0.832111 | 1.14E-55 | -1.69E-46 |
| MS.gene053216 | MS.gene96799 | 0.849685 | 2.76E-60 | -1.69E-46 |
| MS.gene053559 | MS.gene96799 | 0.817215 | 3.70E-52 | -1.69E-46 |
| MS.gene053577 | MS.gene96799 | 0.821341 | 4.25E-53 | -1.69E-46 |
| MS.gene054379 | MS.gene96799 | 0.808764 | 2.64E-50 | -1.69E-46 |
| MS.gene054537 | MS.gene96799 | 0.836904 | 7.12E-57 | -1.69E-46 |
| MS.gene054930 | MS.gene96799 | 0.861299 | 1.13E-63 | -1.69E-46 |
| MS.gene05510 | MS.gene96799 | 0.818867 | 1.57E-52 | -1.69E-46 |
| MS.gene055441 | MS.gene96799 | 0.825874 | 3.70E-54 | -1.69E-46 |
| MS.gene055701 | MS.gene96799 | 0.812445 | 4.22E-51 | -1.69E-46 |
| MS.gene056480 | MS.gene96799 | 0.81157 | 6.55E-51 | -1.69E-46 |
| MS.gene056849 | MS.gene96799 | 0.808332 | 3.26E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene96799 | 0.806726 | 7.15E-50 | -1.69E-46 |
| MS.gene05761 | MS.gene96799 | 0.820986 | 5.13E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene96799 | 0.809744 | 1.63E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene96799 | 0.818759 | 1.66E-52 | -1.69E-46 |
| MS.gene058055 | MS.gene96799 | 0.805532 | 1.28E-49 | -1.69E-46 |
| MS.gene058833 | MS.gene96799 | 0.800222 | 1.59E-48 | -1.69E-46 |
| MS.gene059128 | MS.gene96799 | 0.801446 | 8.97E-49 | -1.69E-46 |
| MS.gene059899 | MS.gene96799 | 0.804123 | 2.51E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene96799 | 0.811943 | 5.44E-51 | -1.69E-46 |
| MS.gene060297 | MS.gene96799 | 0.82556 | 4.39E-54 | -1.69E-46 |
| MS.gene060918 | MS.gene96799 | 0.800681 | 1.29E-48 | -1.69E-46 |
| MS.gene060943 | MS.gene96799 | 0.809774 | 1.60E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene96799 | 0.817371 | 3.41E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96799.t1 | MTR_4g081550 | 97.368 | 114 | 3 | 0 | 1 | 114 | 1 | 114 | 6.57e-78 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGCTTCACGTCCGCGGTTT+TGG | 0.226260 | 4.3:-28541085 | None:intergenic |
| GCGTCGCTAGGTCAGGCTTC+AGG | 0.268972 | 4.3:+28540980 | MS.gene96799:CDS |
| GCCTCTGCACACGTCGGTTT+CGG | 0.293742 | 4.3:+28541104 | MS.gene96799:CDS |
| GACGCCGATGCCGGCGAGCT+TGG | 0.375606 | 4.3:-28540962 | None:intergenic |
| CGTCGCTAGGTCAGGCTTCA+GGG | 0.385353 | 4.3:+28540981 | MS.gene96799:CDS |
| GCAGCCAAGCTCGCCGGCAT+CGG | 0.414205 | 4.3:+28540958 | MS.gene96799:CDS |
| GGCATCGGCGTCGCTAGGTC+AGG | 0.434066 | 4.3:+28540973 | MS.gene96799:CDS |
| TTTCGGAGGATTTGAATGAC+TGG | 0.442439 | 4.3:+28541121 | MS.gene96799:CDS |
| TCGCCGGCATCGGCGTCGCT+AGG | 0.463408 | 4.3:+28540968 | MS.gene96799:CDS |
| CCTACGCCGAGAATGGTGTT+TGG | 0.473022 | 4.3:+28541186 | MS.gene96799:CDS |
| TCCTCCGCTTCCTTCAACGT+CGG | 0.485697 | 4.3:-28541216 | None:intergenic |
| CTGCGGAGAAACAGTGGTCT+CGG | 0.507983 | 4.3:+28541022 | MS.gene96799:CDS |
| TGGAGAGCCTACGCCGAGAA+TGG | 0.509702 | 4.3:+28541179 | MS.gene96799:CDS |
| CGAGACCACTGTTTCTCCGC+AGG | 0.511390 | 4.3:-28541021 | None:intergenic |
| ACCTCTCCAAACACCATTCT+CGG | 0.512931 | 4.3:-28541192 | None:intergenic |
| TGACCTAGCGACGCCGATGC+CGG | 0.514560 | 4.3:-28540971 | None:intergenic |
| CATAAACAAATCACTTTCAT+CGG | 0.525463 | 4.3:-28541151 | None:intergenic |
| TTCGGAGGATTTGAATGACT+GGG | 0.534180 | 4.3:+28541122 | MS.gene96799:CDS |
| CCCTCGTCGGCGATGAGGCG+CGG | 0.537738 | 4.3:-28541047 | None:intergenic |
| AGGACGGCAGCCAAGCTCGC+CGG | 0.539761 | 4.3:+28540952 | MS.gene96799:CDS |
| GATTGGATAATGGGCGGTGG+CGG | 0.542192 | 4.3:+28540922 | None:intergenic |
| AGTGATTTGTTTATGTCGAG+TGG | 0.551557 | 4.3:+28541159 | MS.gene96799:CDS |
| GGTTCCGACGTTGAAGGAAG+CGG | 0.551831 | 4.3:+28541212 | MS.gene96799:CDS |
| TGGAGAGGTTCCGACGTTGA+AGG | 0.586309 | 4.3:+28541206 | MS.gene96799:CDS |
| CGGTGGCGGAACGATGAGGA+CGG | 0.587249 | 4.3:+28540936 | MS.gene96799:CDS |
| GAAGGAAGCGGAGGAAGCCA+CGG | 0.589709 | 4.3:+28541224 | MS.gene96799:CDS |
| ACCGCGCCTCATCGCCGACG+AGG | 0.591542 | 4.3:+28541046 | MS.gene96799:CDS |
| GTCGCTAGGTCAGGCTTCAG+GGG | 0.594901 | 4.3:+28540982 | MS.gene96799:CDS |
| TCTGCACACGTCGGTTTCGG+AGG | 0.596631 | 4.3:+28541107 | MS.gene96799:CDS |
| CGCGTGCTTCAATTCAGCCG+TGG | 0.599375 | 4.3:-28541241 | None:intergenic |
| CCAAACACCATTCTCGGCGT+AGG | 0.600029 | 4.3:-28541186 | None:intergenic |
| CGTATCCTGCGGAGAAACAG+TGG | 0.606045 | 4.3:+28541016 | MS.gene96799:CDS |
| CCGCGCCTCATCGCCGACGA+GGG | 0.606141 | 4.3:+28541047 | MS.gene96799:CDS |
| GTGCAGAGGCTTCACGTCCG+CGG | 0.607881 | 4.3:-28541091 | None:intergenic |
| TCCGAAACCGACGTGTGCAG+AGG | 0.608510 | 4.3:-28541105 | None:intergenic |
| GCCGAGAATGGTGTTTGGAG+AGG | 0.611441 | 4.3:+28541191 | MS.gene96799:CDS |
| GTCAGGCTTCAGGGGAACCA+CGG | 0.616706 | 4.3:+28540990 | MS.gene96799:CDS |
| ACTGATTGGATAATGGGCGG+TGG | 0.623434 | 4.3:+28540919 | None:intergenic |
| CCCTGAGATAAAACCCTCGT+CGG | 0.630010 | 4.3:-28541060 | None:intergenic |
| TGGGCGGTGGCGGAACGATG+AGG | 0.643041 | 4.3:+28540932 | MS.gene96799:CDS |
| TCCGACGTTGAAGGAAGCGG+AGG | 0.652735 | 4.3:+28541215 | MS.gene96799:CDS |
| TAAAACCCTCGTCGGCGATG+AGG | 0.653238 | 4.3:-28541052 | None:intergenic |
| ATCTCAGGGGACCAAAACCG+CGG | 0.669256 | 4.3:+28541074 | MS.gene96799:CDS |
| GCAACTGATTGGATAATGGG+CGG | 0.691401 | 4.3:+28540916 | None:intergenic |
| CTCCGCAGGATACGTAACCG+TGG | 0.700047 | 4.3:-28541007 | None:intergenic |
| CGTGAAGCCTCTGCACACGT+CGG | 0.703926 | 4.3:+28541098 | MS.gene96799:CDS |
| AACCACGGTTACGTATCCTG+CGG | 0.727599 | 4.3:+28541005 | MS.gene96799:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CATAAACAAATCACTTTCAT+CGG | - | chr4.3:28541154-28541173 | None:intergenic | 25.0% |
| !! | AGTGATTTGTTTATGTCGAG+TGG | + | chr4.3:28541159-28541178 | MS.gene96799:CDS | 35.0% |
| TTCGGAGGATTTGAATGACT+GGG | + | chr4.3:28541122-28541141 | MS.gene96799:CDS | 40.0% | |
| TTTCGGAGGATTTGAATGAC+TGG | + | chr4.3:28541121-28541140 | MS.gene96799:CDS | 40.0% | |
| ACCTCTCCAAACACCATTCT+CGG | - | chr4.3:28541195-28541214 | None:intergenic | 45.0% | |
| AACCACGGTTACGTATCCTG+CGG | + | chr4.3:28541005-28541024 | MS.gene96799:CDS | 50.0% | |
| CCCTGAGATAAAACCCTCGT+CGG | - | chr4.3:28541063-28541082 | None:intergenic | 50.0% | |
| !! | CCGACGAGGGTTTTATCTCA+GGG | + | chr4.3:28541060-28541079 | MS.gene96799:CDS | 50.0% |
| !! | CGACGAGGGTTTTATCTCAG+GGG | + | chr4.3:28541061-28541080 | MS.gene96799:CDS | 50.0% |
| ATCTCAGGGGACCAAAACCG+CGG | + | chr4.3:28541074-28541093 | MS.gene96799:CDS | 55.0% | |
| CCAAACACCATTCTCGGCGT+AGG | - | chr4.3:28541189-28541208 | None:intergenic | 55.0% | |
| CCTACGCCGAGAATGGTGTT+TGG | + | chr4.3:28541186-28541205 | MS.gene96799:CDS | 55.0% | |
| CGTATCCTGCGGAGAAACAG+TGG | + | chr4.3:28541016-28541035 | MS.gene96799:CDS | 55.0% | |
| CTGCGGAGAAACAGTGGTCT+CGG | + | chr4.3:28541022-28541041 | MS.gene96799:CDS | 55.0% | |
| GCCGAGAATGGTGTTTGGAG+AGG | + | chr4.3:28541191-28541210 | MS.gene96799:CDS | 55.0% | |
| GGTTCCGACGTTGAAGGAAG+CGG | + | chr4.3:28541212-28541231 | MS.gene96799:CDS | 55.0% | |
| TAAAACCCTCGTCGGCGATG+AGG | - | chr4.3:28541055-28541074 | None:intergenic | 55.0% | |
| TCCTCCGCTTCCTTCAACGT+CGG | - | chr4.3:28541219-28541238 | None:intergenic | 55.0% | |
| TGGAGAGGTTCCGACGTTGA+AGG | + | chr4.3:28541206-28541225 | MS.gene96799:CDS | 55.0% | |
| !! | GCCGACGAGGGTTTTATCTC+AGG | + | chr4.3:28541059-28541078 | MS.gene96799:CDS | 55.0% |
| CGCGTGCTTCAATTCAGCCG+TGG | - | chr4.3:28541244-28541263 | None:intergenic | 60.0% | |
| CGTCGCTAGGTCAGGCTTCA+GGG | + | chr4.3:28540981-28541000 | MS.gene96799:CDS | 60.0% | |
| CGTGAAGCCTCTGCACACGT+CGG | + | chr4.3:28541098-28541117 | MS.gene96799:CDS | 60.0% | |
| CTCCGCAGGATACGTAACCG+TGG | - | chr4.3:28541010-28541029 | None:intergenic | 60.0% | |
| GAAGGAAGCGGAGGAAGCCA+CGG | + | chr4.3:28541224-28541243 | MS.gene96799:CDS | 60.0% | |
| GTCAGGCTTCAGGGGAACCA+CGG | + | chr4.3:28540990-28541009 | MS.gene96799:CDS | 60.0% | |
| GTCGCTAGGTCAGGCTTCAG+GGG | + | chr4.3:28540982-28541001 | MS.gene96799:CDS | 60.0% | |
| TCCGAAACCGACGTGTGCAG+AGG | - | chr4.3:28541108-28541127 | None:intergenic | 60.0% | |
| TCCGACGTTGAAGGAAGCGG+AGG | + | chr4.3:28541215-28541234 | MS.gene96799:CDS | 60.0% | |
| TGGAGAGCCTACGCCGAGAA+TGG | + | chr4.3:28541179-28541198 | MS.gene96799:CDS | 60.0% | |
| ! | AGGCTTCACGTCCGCGGTTT+TGG | - | chr4.3:28541088-28541107 | None:intergenic | 60.0% |
| ! | CGAGACCACTGTTTCTCCGC+AGG | - | chr4.3:28541024-28541043 | None:intergenic | 60.0% |
| !! | GCCTCTGCACACGTCGGTTT+CGG | + | chr4.3:28541104-28541123 | MS.gene96799:CDS | 60.0% |
| !! | TCTGCACACGTCGGTTTCGG+AGG | + | chr4.3:28541107-28541126 | MS.gene96799:CDS | 60.0% |
| GCGTCGCTAGGTCAGGCTTC+AGG | + | chr4.3:28540980-28540999 | MS.gene96799:CDS | 65.0% | |
| GTGCAGAGGCTTCACGTCCG+CGG | - | chr4.3:28541094-28541113 | None:intergenic | 65.0% | |
| TGACCTAGCGACGCCGATGC+CGG | - | chr4.3:28540974-28540993 | None:intergenic | 65.0% | |
| !! | CGGTGGCGGAACGATGAGGA+CGG | + | chr4.3:28540936-28540955 | MS.gene96799:CDS | 65.0% |
| AGGACGGCAGCCAAGCTCGC+CGG | + | chr4.3:28540952-28540971 | MS.gene96799:CDS | 70.0% | |
| GCAGCCAAGCTCGCCGGCAT+CGG | + | chr4.3:28540958-28540977 | MS.gene96799:CDS | 70.0% | |
| !! | GGCATCGGCGTCGCTAGGTC+AGG | + | chr4.3:28540973-28540992 | MS.gene96799:CDS | 70.0% |
| !! | TGGGCGGTGGCGGAACGATG+AGG | + | chr4.3:28540932-28540951 | MS.gene96799:CDS | 70.0% |
| ACCGCGCCTCATCGCCGACG+AGG | + | chr4.3:28541046-28541065 | MS.gene96799:CDS | 75.0% | |
| CCCTCGTCGGCGATGAGGCG+CGG | - | chr4.3:28541050-28541069 | None:intergenic | 75.0% | |
| CCGCGCCTCATCGCCGACGA+GGG | + | chr4.3:28541047-28541066 | MS.gene96799:CDS | 75.0% | |
| GACGCCGATGCCGGCGAGCT+TGG | - | chr4.3:28540965-28540984 | None:intergenic | 75.0% | |
| !! | TCGCCGGCATCGGCGTCGCT+AGG | + | chr4.3:28540968-28540987 | MS.gene96799:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 28540931 | 28541275 | 28540931 | ID=MS.gene96799 |
| chr4.3 | mRNA | 28540931 | 28541275 | 28540931 | ID=MS.gene96799.t1;Parent=MS.gene96799 |
| chr4.3 | exon | 28540931 | 28541275 | 28540931 | ID=MS.gene96799.t1.exon1;Parent=MS.gene96799.t1 |
| chr4.3 | CDS | 28540931 | 28541275 | 28540931 | ID=cds.MS.gene96799.t1;Parent=MS.gene96799.t1 |
| Gene Sequence |
| Protein sequence |
