AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96810.t1 MTR_4g081420 99.303 287 1 1 1 286 151 437 0.0 582
MS.gene96810.t1 MTR_2g010860 53.497 286 123 6 4 285 97 376 3.93e-92 278
MS.gene96810.t1 MTR_2g010870 54.710 276 111 5 22 285 82 355 1.53e-91 276
MS.gene96810.t1 MTR_3g072920 51.653 242 103 7 49 283 214 448 8.76e-73 230
MS.gene96810.t1 MTR_1g024980 51.261 238 103 3 49 283 171 398 7.27e-72 226
MS.gene96810.t1 MTR_3g463270 44.583 240 116 8 54 283 154 386 2.44e-57 189
MS.gene96810.t1 MTR_5g012220 34.375 224 94 7 76 285 105 289 2.07e-31 118
MS.gene96810.t1 MTR_4g101450 35.238 210 100 6 79 285 98 274 4.21e-29 112
MS.gene96810.t1 MTR_5g094440 32.864 213 129 5 73 283 68 268 3.63e-27 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96810.t1 AT5G54630 71.711 304 64 7 1 285 171 471 1.17e-144 415
MS.gene96810.t1 AT4G27240 76.357 258 58 3 31 285 173 430 1.07e-138 398
MS.gene96810.t1 AT4G27240 75.217 230 54 3 31 257 173 402 7.03e-118 345
MS.gene96810.t1 AT1G11490 50.598 251 110 5 44 285 119 364 1.52e-74 232
MS.gene96810.t1 AT1G11490 50.598 251 110 5 44 285 119 364 1.52e-74 232
MS.gene96810.t1 AT1G75710 46.875 256 109 5 54 285 206 458 3.23e-63 206
MS.gene96810.t1 AT2G29660 41.634 257 125 7 46 283 120 370 2.04e-50 170
MS.gene96810.t1 AT4G22560 38.605 215 86 9 73 283 89 261 2.25e-30 115
MS.gene96810.t1 AT1G62520 38.863 211 96 7 77 285 100 279 3.53e-30 115
MS.gene96810.t1 AT4G12450 33.180 217 96 7 77 283 97 274 1.54e-24 100

Find 49 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAACTGGATTTGATTCTT+TGG 0.236240 4.3:-28644086 MS.gene96810:CDS
CTTGGCAGTTACTGAACTTT+TGG 0.259869 4.3:-28644608 MS.gene96810:intron
GACACCTAGGATGTTTCTTC+TGG 0.309460 4.3:+28644416 None:intergenic
ACCACAGTAGCTTGTTCTCT+TGG 0.380190 4.3:-28644358 MS.gene96810:CDS
TCTCTTGAAACAGATTCTAA+TGG 0.386384 4.3:-28645125 MS.gene96810:CDS
GCTTGTTCTCTTGGTCTCAA+TGG 0.401559 4.3:-28644349 MS.gene96810:CDS
AAAATATTTACCAGCATGCT+TGG 0.405089 4.3:+28645028 None:intergenic
AAGGAGTTGAAAGGTGGAAT+AGG 0.409316 4.3:-28644256 MS.gene96810:CDS
GTTGTGTTTGTAGAATCATT+AGG 0.411461 4.3:-28644297 MS.gene96810:CDS
TAATTGCTGGTAGGGTTCAT+AGG 0.416479 4.3:-28644138 MS.gene96810:CDS
ACTGGATTTGATTCTTTGGC+TGG 0.418812 4.3:-28644082 MS.gene96810:CDS
CAAGAAATGGCATCTCAAAC+TGG 0.425597 4.3:-28644100 MS.gene96810:CDS
GTGGTGTTCATTCAAGTAAT+AGG 0.426313 4.3:-28645151 MS.gene96810:CDS
AAAAGAAAAGGTGATTTCCT+TGG 0.433309 4.3:+28645203 None:intergenic
ACTTGAAAACATTCAAGAAA+TGG 0.445655 4.3:-28644113 MS.gene96810:CDS
GTGGTGAACAGTTTAACAAA+TGG 0.446542 4.3:-28645076 MS.gene96810:CDS
TAATGAACCTTCACTTAGAA+AGG 0.446634 4.3:-28644179 MS.gene96810:CDS
GATTCTTTGGCTGGTAAAGT+AGG 0.457682 4.3:-28644073 MS.gene96810:CDS
GTTGAAGAAGTCCTAAAAGA+AGG 0.458747 4.3:+28645230 None:intergenic
ATGCAGAGTAATTGCTGGTA+GGG 0.484307 4.3:-28644146 MS.gene96810:CDS
TACCAGCATGCTTGGAGAGA+TGG 0.491155 4.3:+28645036 None:intergenic
ATAGTATGCAGAGTAATTGC+TGG 0.496317 4.3:-28644151 MS.gene96810:CDS
TGGTGAACAGTTTAACAAAT+GGG 0.498621 4.3:-28645075 MS.gene96810:CDS
GACCCTTCTGACAAAAGAAA+AGG 0.499545 4.3:+28645191 None:intergenic
ATCAATGCCTTTCTAAGTGA+AGG 0.505745 4.3:+28644172 None:intergenic
CATCCTAGGTGTCTAGCTGA+TGG 0.521265 4.3:-28644406 MS.gene96810:CDS
CACCATCTCTCCAAGCATGC+TGG 0.523843 4.3:-28645038 MS.gene96810:intron
AAACAAGGAAGAAGAGCTCT+AGG 0.526190 4.3:+28644013 None:intergenic
AAATTATATGCAGGACAAGC+TGG 0.530091 4.3:-28644558 MS.gene96810:CDS
AGGTCTTTATTCAAACATTG+AGG 0.530157 4.3:-28644053 MS.gene96810:CDS
AGATTCATCAAGGAAAATAG+TGG 0.534245 4.3:-28644581 MS.gene96810:CDS
AGAAAAGGTGATTTCCTTGG+AGG 0.540659 4.3:+28645206 None:intergenic
CACAACATCTACAAGTGGAA+GGG 0.551250 4.3:-28644227 MS.gene96810:CDS
GGACTTCTTCAACTCCTCCA+AGG 0.558541 4.3:-28645220 MS.gene96810:CDS
TATGCAGAGTAATTGCTGGT+AGG 0.562940 4.3:-28644147 MS.gene96810:CDS
TCACAACATCTACAAGTGGA+AGG 0.571126 4.3:-28644228 MS.gene96810:CDS
AACTCCAGAAGAAACATCCT+AGG 0.571187 4.3:-28644420 MS.gene96810:CDS
AAATAGTGGAAATTATATGC+AGG 0.573880 4.3:-28644567 MS.gene96810:CDS
TTGAAGAAGTCCTAAAAGAA+GGG 0.580726 4.3:+28645231 None:intergenic
TCTGCTAAGAAGGAGTTGAA+AGG 0.586835 4.3:-28644265 MS.gene96810:CDS
ATTTGAAGAGTACAGAGAGA+TGG 0.595060 4.3:-28644461 MS.gene96810:CDS
GTTTGTAGAATCATTAGGAG+TGG 0.602627 4.3:-28644292 MS.gene96810:CDS
TTGCAAATTACCACAAAACA+AGG 0.607767 4.3:+28643998 None:intergenic
TTTCCATCAGCTAGACACCT+AGG 0.608502 4.3:+28644403 None:intergenic
GCTAAGAAGGAGTTGAAAGG+TGG 0.609494 4.3:-28644262 MS.gene96810:CDS
CTCTCTCTATTCTGCCACAG+TGG 0.632803 4.3:+28644521 None:intergenic
ACCAAGAGAACAAGCTACTG+TGG 0.672385 4.3:+28644357 None:intergenic
ACTGTTACTTGTCACAAATG+TGG 0.687396 4.3:-28645095 MS.gene96810:CDS
TTAAAGTCAGAAAACCACTG+TGG 0.819817 4.3:-28644535 MS.gene96810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTTCAAAATATCT+AGG - chr4.3:28644444-28644463 MS.gene96810:CDS 10.0%
!!! TTTTTATTAAAAATTCTTCT+TGG - chr4.3:28644229-28644248 MS.gene96810:CDS 10.0%
!! CAAACAATTCTAAATTAAAA+AGG + chr4.3:28644411-28644430 None:intergenic 15.0%
!!! ATTTTGAAAATAAAATCTGT+TGG + chr4.3:28644440-28644459 None:intergenic 15.0%
!!! TATGCTATAGAATTTTTTTA+TGG - chr4.3:28644311-28644330 MS.gene96810:CDS 15.0%
!! AATTCTAAATTAAAAAGGCA+TGG + chr4.3:28644406-28644425 None:intergenic 20.0%
! AATACTAGGATAAAGTAGAT+TGG + chr4.3:28644496-28644515 None:intergenic 25.0%
! ACTTGAAAACATTCAAGAAA+TGG - chr4.3:28645123-28645142 MS.gene96810:CDS 25.0%
! TGTTCAATGTTCAAAATACT+AGG + chr4.3:28644510-28644529 None:intergenic 25.0%
! TTTGTGGTCAGAAAAATATT+TGG + chr4.3:28644266-28644285 None:intergenic 25.0%
!! AAATAGTGGAAATTATATGC+AGG - chr4.3:28644669-28644688 MS.gene96810:intron 25.0%
!! ATTACTTTAACCCTTCTTTT+AGG - chr4.3:28643995-28644014 MS.gene96810:CDS 25.0%
!! TAAAAATTCTTCTTGGCTTT+AGG - chr4.3:28644236-28644255 MS.gene96810:CDS 25.0%
!!! AAATCCACACTTTTTGAATT+TGG - chr4.3:28644355-28644374 MS.gene96810:CDS 25.0%
!!! AATCCACACTTTTTGAATTT+GGG - chr4.3:28644356-28644375 MS.gene96810:CDS 25.0%
!!! AGAATAATTTGTTTTTCCCT+TGG - chr4.3:28644610-28644629 MS.gene96810:intron 25.0%
!!! ATTTCTTGAATGTTTTCAAG+TGG + chr4.3:28645124-28645143 None:intergenic 25.0%
!!! CTAAAGTTTTTTGCATATTG+TGG + chr4.3:28644751-28644770 None:intergenic 25.0%
!!! GCTATAGAATTTTTTTATGG+TGG - chr4.3:28644314-28644333 MS.gene96810:CDS 25.0%
AAAAGAAAAGGTGATTTCCT+TGG + chr4.3:28644036-28644055 None:intergenic 30.0%
AAAATATTTACCAGCATGCT+TGG + chr4.3:28644211-28644230 None:intergenic 30.0%
AGATTCATCAAGGAAAATAG+TGG - chr4.3:28644655-28644674 MS.gene96810:intron 30.0%
AGCATATCAGATAAAGACTA+TGG + chr4.3:28644297-28644316 None:intergenic 30.0%
AGGATAAAGTAGATTGGAAA+AGG + chr4.3:28644490-28644509 None:intergenic 30.0%
AGGTCTTTATTCAAACATTG+AGG - chr4.3:28645183-28645202 MS.gene96810:CDS 30.0%
GGATAAAGTAGATTGGAAAA+GGG + chr4.3:28644489-28644508 None:intergenic 30.0%
GTTGTGTTTGTAGAATCATT+AGG - chr4.3:28644939-28644958 MS.gene96810:intron 30.0%
TAATGAACCTTCACTTAGAA+AGG - chr4.3:28645057-28645076 MS.gene96810:CDS 30.0%
TCAAACTGGATTTGATTCTT+TGG - chr4.3:28645150-28645169 MS.gene96810:CDS 30.0%
TCTCTTGAAACAGATTCTAA+TGG - chr4.3:28644111-28644130 MS.gene96810:CDS 30.0%
TGGTGAACAGTTTAACAAAT+GGG - chr4.3:28644161-28644180 MS.gene96810:CDS 30.0%
TTGAAGAAGTCCTAAAAGAA+GGG + chr4.3:28644008-28644027 None:intergenic 30.0%
TTGCAAATTACCACAAAACA+AGG + chr4.3:28645241-28645260 None:intergenic 30.0%
! AATGAACTTTTACGGTTCTA+TGG - chr4.3:28644854-28644873 MS.gene96810:intron 30.0%
! CTGATGGAAATGAACTTTTA+CGG - chr4.3:28644846-28644865 MS.gene96810:intron 30.0%
! TTTTCTGACCACAAACTTTT+TGG - chr4.3:28644271-28644290 MS.gene96810:CDS 30.0%
!! CAGAATAGAGAGAGTTTTAA+AGG - chr4.3:28644724-28644743 MS.gene96810:intron 30.0%
AAAAGTTCAGTAACTGCCAA+GGG + chr4.3:28644629-28644648 None:intergenic 35.0%
AAATTATATGCAGGACAAGC+TGG - chr4.3:28644678-28644697 MS.gene96810:intron 35.0%
AGTCCCAAATTCAAAAAGTG+TGG + chr4.3:28644362-28644381 None:intergenic 35.0%
ATAGTATGCAGAGTAATTGC+TGG - chr4.3:28645085-28645104 MS.gene96810:CDS 35.0%
ATCAATGCCTTTCTAAGTGA+AGG + chr4.3:28645067-28645086 None:intergenic 35.0%
GTGGTGAACAGTTTAACAAA+TGG - chr4.3:28644160-28644179 MS.gene96810:CDS 35.0%
GTGGTGTTCATTCAAGTAAT+AGG - chr4.3:28644085-28644104 MS.gene96810:CDS 35.0%
GTTGAAGAAGTCCTAAAAGA+AGG + chr4.3:28644009-28644028 None:intergenic 35.0%
GTTTGTAGAATCATTAGGAG+TGG - chr4.3:28644944-28644963 MS.gene96810:intron 35.0%
TTAAAGTCAGAAAACCACTG+TGG - chr4.3:28644701-28644720 MS.gene96810:intron 35.0%
! ACTGTTACTTGTCACAAATG+TGG - chr4.3:28644141-28644160 MS.gene96810:CDS 35.0%
! ATTTGAAGAGTACAGAGAGA+TGG - chr4.3:28644775-28644794 MS.gene96810:intron 35.0%
! CACCTTTTCTTTTGTCAGAA+GGG - chr4.3:28644043-28644062 MS.gene96810:CDS 35.0%
! GTTTTCACAACATCTACAAG+TGG - chr4.3:28645004-28645023 MS.gene96810:intron 35.0%
! TCACCTTTTCTTTTGTCAGA+AGG - chr4.3:28644042-28644061 MS.gene96810:CDS 35.0%
!! TTTTGGAAGGAGATTCATCA+AGG - chr4.3:28644645-28644664 MS.gene96810:intron 35.0%
AAACAAGGAAGAAGAGCTCT+AGG + chr4.3:28645226-28645245 None:intergenic 40.0%
AACTCCAGAAGAAACATCCT+AGG - chr4.3:28644816-28644835 MS.gene96810:intron 40.0%
AAGGAGTTGAAAGGTGGAAT+AGG - chr4.3:28644980-28644999 MS.gene96810:intron 40.0%
ACTGGATTTGATTCTTTGGC+TGG - chr4.3:28645154-28645173 MS.gene96810:CDS 40.0%
AGAAAAGGTGATTTCCTTGG+AGG + chr4.3:28644033-28644052 None:intergenic 40.0%
ATGCAGAGTAATTGCTGGTA+GGG - chr4.3:28645090-28645109 MS.gene96810:CDS 40.0%
CAAAAGTTCAGTAACTGCCA+AGG + chr4.3:28644630-28644649 None:intergenic 40.0%
CAAGAAATGGCATCTCAAAC+TGG - chr4.3:28645136-28645155 MS.gene96810:CDS 40.0%
CACAACATCTACAAGTGGAA+GGG - chr4.3:28645009-28645028 MS.gene96810:intron 40.0%
GACCCTTCTGACAAAAGAAA+AGG + chr4.3:28644048-28644067 None:intergenic 40.0%
GACTATGGCCAAAAAGTTTG+TGG + chr4.3:28644282-28644301 None:intergenic 40.0%
GATTCTTTGGCTGGTAAAGT+AGG - chr4.3:28645163-28645182 MS.gene96810:CDS 40.0%
TAATTGCTGGTAGGGTTCAT+AGG - chr4.3:28645098-28645117 MS.gene96810:CDS 40.0%
TATGCAGAGTAATTGCTGGT+AGG - chr4.3:28645089-28645108 MS.gene96810:CDS 40.0%
TCACAACATCTACAAGTGGA+AGG - chr4.3:28645008-28645027 MS.gene96810:intron 40.0%
TCTGCTAAGAAGGAGTTGAA+AGG - chr4.3:28644971-28644990 MS.gene96810:intron 40.0%
! CTTGGCAGTTACTGAACTTT+TGG - chr4.3:28644628-28644647 MS.gene96810:intron 40.0%
! CTTTTGTCAGAAGGGTCTAA+TGG - chr4.3:28644051-28644070 MS.gene96810:CDS 40.0%
! GAAGGGTCTAATGGTTTTCA+TGG - chr4.3:28644060-28644079 MS.gene96810:CDS 40.0%
! GAGTGGTTTTTCTGCTAAGA+AGG - chr4.3:28644961-28644980 MS.gene96810:intron 40.0%
! GCAGTTACTGAACTTTTGGA+AGG - chr4.3:28644632-28644651 MS.gene96810:intron 40.0%
! TCTAATGGTTTTCATGGTGG+TGG - chr4.3:28644066-28644085 MS.gene96810:CDS 40.0%
!!! AGCTCTTCTTCCTTGTTTTG+TGG - chr4.3:28645228-28645247 MS.gene96810:CDS 40.0%
ACCAAGAGAACAAGCTACTG+TGG + chr4.3:28644882-28644901 None:intergenic 45.0%
GACACCTAGGATGTTTCTTC+TGG + chr4.3:28644823-28644842 None:intergenic 45.0%
GCTAAGAAGGAGTTGAAAGG+TGG - chr4.3:28644974-28644993 MS.gene96810:intron 45.0%
GCTTGTTCTCTTGGTCTCAA+TGG - chr4.3:28644887-28644906 MS.gene96810:intron 45.0%
TTTCCATCAGCTAGACACCT+AGG + chr4.3:28644836-28644855 None:intergenic 45.0%
! GGGTCTAATGGTTTTCATGG+TGG - chr4.3:28644063-28644082 MS.gene96810:CDS 45.0%
!! ACCACAGTAGCTTGTTCTCT+TGG - chr4.3:28644878-28644897 MS.gene96810:intron 45.0%
CATCCTAGGTGTCTAGCTGA+TGG - chr4.3:28644830-28644849 MS.gene96810:intron 50.0%
CTCTCTCTATTCTGCCACAG+TGG + chr4.3:28644718-28644737 None:intergenic 50.0%
GGACTTCTTCAACTCCTCCA+AGG - chr4.3:28644016-28644035 MS.gene96810:CDS 50.0%
TACCAGCATGCTTGGAGAGA+TGG + chr4.3:28644203-28644222 None:intergenic 50.0%
CACCATCTCTCCAAGCATGC+TGG - chr4.3:28644198-28644217 MS.gene96810:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 28643991 28645267 28643991 ID=MS.gene96810
chr4.3 mRNA 28643991 28645267 28643991 ID=MS.gene96810.t1;Parent=MS.gene96810
chr4.3 exon 28645039 28645267 28645039 ID=MS.gene96810.t1.exon1;Parent=MS.gene96810.t1
chr4.3 CDS 28645039 28645267 28645039 ID=cds.MS.gene96810.t1;Parent=MS.gene96810.t1
chr4.3 exon 28643991 28644622 28643991 ID=MS.gene96810.t1.exon2;Parent=MS.gene96810.t1
chr4.3 CDS 28643991 28644622 28643991 ID=cds.MS.gene96810.t1;Parent=MS.gene96810.t1
Gene Sequence

>MS.gene96810

ATGCATTACTTTAACCCTTCTTTTAGGACTTCTTCAACTCCTCCAAGGAAATCACCTTTTCTTTTGTCAGAAGGGTCTAATGGTTTTCATGGTGGTGGTGTTCATTCAAGTAATAGGATGTCTCTTGAAACAGATTCTAATGGTTCTTCAACTGTTACTTGTCACAAATGTGGTGAACAGTTTAACAAATGGGAAGCTGCTGAAGCTCACCATCTCTCCAAGCATGCTGGTAAATATTTTTTTATTAAAAATTCTTCTTGGCTTTAGGTTATCCAAATATTTTTCTGACCACAAACTTTTTGGCCATAGTCTTTATCTGATATGCTATAGAATTTTTTTATGGTGGTGTGACAAATCTTACAATAAATCCACACTTTTTGAATTTGGGACTGTAAAGCTGATTTTGCTTTTTCCATGCCTTTTTAATTTAGAATTGTTTGTATTCTCCAACAGATTTTATTTTCAAAATATCTAGGATAAATAAAAGTACTATCTCCCTTTTCCAATCTACTTTATCCTAGTATTTTGAACATTGAACAATAGTGTATCAAAGAACAATAGAGATTTGTTCAAGTTTGTTGTTATGACATTTTGAGTTTCATAAATTTTGAATTTTAAGAGAATAATTTGTTTTTCCCTTGGCAGTTACTGAACTTTTGGAAGGAGATTCATCAAGGAAAATAGTGGAAATTATATGCAGGACAAGCTGGTTAAAGTCAGAAAACCACTGTGGCAGAATAGAGAGAGTTTTAAAGGTCCACAATATGCAAAAAACTTTAGCTAGATTTGAAGAGTACAGAGAGATGGTAAAAATCAAAGCAAGTAAACTCCAGAAGAAACATCCTAGGTGTCTAGCTGATGGAAATGAACTTTTACGGTTCTATGGAACCACAGTAGCTTGTTCTCTTGGTCTCAATGGCTCTTCAAGTCTTTGTTTATCAGAAAAATGTTGTGTTTGTAGAATCATTAGGAGTGGTTTTTCTGCTAAGAAGGAGTTGAAAGGTGGAATAGGTGTTTTCACAACATCTACAAGTGGAAGGGCTTTTGAATCTATAGAAATTTTTGATAATGAACCTTCACTTAGAAAGGCATTGATAGTATGCAGAGTAATTGCTGGTAGGGTTCATAGGCCACTTGAAAACATTCAAGAAATGGCATCTCAAACTGGATTTGATTCTTTGGCTGGTAAAGTAGGTCTTTATTCAAACATTGAGGAGCTTTATTTGCTCAATCCTAGAGCTCTTCTTCCTTGTTTTGTGGTAATTTGCAAATCATGA

Protein sequence

>MS.gene96810.t1

MHYFNPSFRTSSTPPRKSPFLLSEGSNGFHGGGVHSSNRMSLETDSNGSSTVTCHKCGEQFNKWEAAEAHHLSKHAVTELLEGDSSRKIVEIICRTSWLKSENHCGRIERVLKVHNMQKTLARFEEYREMVKIKASKLQKKHPRCLADGNELLRFYGTTVACSLGLNGSSSLCLSEKCCVCRIIRSGFSAKKELKGGIGVFTTSTSGRAFESIEIFDNEPSLRKALIVCRVIAGRVHRPLENIQEMASQTGFDSLAGKVGLYSNIEELYLLNPRALLPCFVVICKS