Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97457.t1 | XP_003621016.1 | 92.2 | 335 | 24 | 2 | 126 | 460 | 12 | 344 | 1.10E-164 | 589.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97457.t1 | Q8IXU6 | 42.6 | 277 | 152 | 2 | 145 | 414 | 56 | 332 | 1.1e-59 | 232.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97457.t1 | G7KWQ4 | 92.2 | 335 | 24 | 2 | 126 | 460 | 12 | 344 | 8.3e-165 | 589.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene97457 | 0.823171 | 1.60E-53 | -1.69E-46 |
| MS.gene049343 | MS.gene97457 | 0.807258 | 5.52E-50 | -1.69E-46 |
| MS.gene049404 | MS.gene97457 | 0.810113 | 1.35E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene97457 | 0.808923 | 2.44E-50 | -1.69E-46 |
| MS.gene05016 | MS.gene97457 | 0.803654 | 3.14E-49 | -1.69E-46 |
| MS.gene050273 | MS.gene97457 | 0.812344 | 4.45E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene97457 | 0.81504 | 1.13E-51 | -1.69E-46 |
| MS.gene050561 | MS.gene97457 | 0.813872 | 2.05E-51 | -1.69E-46 |
| MS.gene050562 | MS.gene97457 | 0.804312 | 2.29E-49 | -1.69E-46 |
| MS.gene050759 | MS.gene97457 | 0.816283 | 5.99E-52 | -1.69E-46 |
| MS.gene050962 | MS.gene97457 | 0.810988 | 8.76E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene97457 | 0.814606 | 1.41E-51 | -1.69E-46 |
| MS.gene051594 | MS.gene97457 | 0.803985 | 2.68E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene97457 | 0.801055 | 1.08E-48 | -1.69E-46 |
| MS.gene051744 | MS.gene97457 | 0.807557 | 4.77E-50 | -1.69E-46 |
| MS.gene052037 | MS.gene97457 | 0.80388 | 2.82E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene97457 | 0.801492 | 8.78E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene97457 | 0.817513 | 3.17E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene97457 | 0.811296 | 7.51E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene97457 | 0.803626 | 3.19E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene97457 | 0.810884 | 9.23E-51 | -1.69E-46 |
| MS.gene052863 | MS.gene97457 | 0.801073 | 1.07E-48 | -1.69E-46 |
| MS.gene053006 | MS.gene97457 | 0.806793 | 6.92E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene97457 | 0.804359 | 2.24E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene97457 | 0.820457 | 6.79E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene97457 | -0.815749 | 7.88E-52 | -1.69E-46 |
| MS.gene054467 | MS.gene97457 | 0.810201 | 1.30E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene97457 | 0.814459 | 1.52E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene97457 | 0.803244 | 3.82E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene97457 | 0.848116 | 7.52E-60 | -1.69E-46 |
| MS.gene055227 | MS.gene97457 | 0.807713 | 4.42E-50 | -1.69E-46 |
| MS.gene055512 | MS.gene97457 | 0.801551 | 8.54E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene97457 | 0.811311 | 7.46E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene97457 | 0.839394 | 1.63E-57 | -1.69E-46 |
| MS.gene055926 | MS.gene97457 | 0.805315 | 1.42E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene97457 | 0.812856 | 3.43E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene97457 | 0.801398 | 9.17E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene97457 | 0.819608 | 1.06E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene97457 | 0.802769 | 4.79E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene97457 | 0.847005 | 1.52E-59 | -1.69E-46 |
| MS.gene058979 | MS.gene97457 | 0.805259 | 1.46E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene97457 | 0.805857 | 1.09E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene54388 | MS.gene97457 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97457.t1 | MTR_7g006080 | 91.691 | 337 | 26 | 2 | 124 | 460 | 10 | 344 | 0.0 | 617 |
| MS.gene97457.t1 | MTR_7g104290 | 51.020 | 294 | 139 | 3 | 140 | 431 | 30 | 320 | 2.51e-94 | 289 |
| MS.gene97457.t1 | MTR_7g104300 | 49.831 | 295 | 142 | 3 | 140 | 431 | 30 | 321 | 1.30e-90 | 279 |
| MS.gene97457.t1 | MTR_7g006100 | 75.424 | 118 | 16 | 1 | 162 | 279 | 258 | 362 | 1.49e-49 | 181 |
| MS.gene97457.t1 | MTR_8g096720 | 46.250 | 80 | 43 | 0 | 1 | 80 | 1 | 80 | 6.31e-14 | 71.6 |
| MS.gene97457.t1 | MTR_5g036320 | 50.000 | 64 | 31 | 1 | 94 | 157 | 49 | 111 | 2.76e-13 | 67.4 |
| MS.gene97457.t1 | MTR_5g049680 | 44.262 | 61 | 34 | 0 | 97 | 157 | 3 | 63 | 1.19e-11 | 61.6 |
| MS.gene97457.t1 | MTR_8g031080 | 46.667 | 60 | 27 | 1 | 91 | 150 | 260 | 314 | 2.95e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97457.t1 | AT3G59310 | 69.072 | 291 | 89 | 1 | 147 | 437 | 32 | 321 | 2.02e-147 | 424 |
| MS.gene97457.t1 | AT3G59310 | 62.422 | 322 | 89 | 2 | 147 | 437 | 32 | 352 | 4.19e-141 | 409 |
| MS.gene97457.t1 | AT3G59340 | 70.480 | 271 | 79 | 1 | 146 | 416 | 61 | 330 | 5.47e-141 | 408 |
| MS.gene97457.t1 | AT3G59340 | 70.480 | 271 | 79 | 1 | 146 | 416 | 61 | 330 | 5.47e-141 | 408 |
| MS.gene97457.t1 | AT3G59340 | 70.480 | 271 | 79 | 1 | 146 | 416 | 61 | 330 | 5.47e-141 | 408 |
| MS.gene97457.t1 | AT3G59340 | 70.480 | 271 | 79 | 1 | 146 | 416 | 34 | 303 | 7.11e-141 | 407 |
| MS.gene97457.t1 | AT3G59310 | 64.605 | 291 | 84 | 2 | 147 | 437 | 32 | 303 | 6.70e-132 | 384 |
| MS.gene97457.t1 | AT3G59320 | 62.925 | 294 | 109 | 0 | 154 | 447 | 39 | 332 | 4.14e-119 | 352 |
| MS.gene97457.t1 | AT3G59330 | 73.077 | 182 | 44 | 2 | 151 | 329 | 59 | 238 | 5.07e-88 | 269 |
| MS.gene97457.t1 | AT3G59330 | 73.184 | 179 | 43 | 2 | 151 | 326 | 59 | 235 | 6.22e-87 | 266 |
| MS.gene97457.t1 | AT3G59320 | 61.207 | 232 | 90 | 0 | 217 | 448 | 1 | 232 | 7.88e-86 | 263 |
| MS.gene97457.t1 | AT3G59330 | 70.879 | 182 | 39 | 2 | 151 | 329 | 59 | 229 | 3.12e-83 | 257 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50664 | gene | 9515 | 17311 | 9515 | ID=MS.gene97457 |
| 50664 | mRNA | 9515 | 17311 | 9515 | ID=MS.gene97457.t1;Parent=MS.gene97457 |
| 50664 | exon | 9515 | 9754 | 9515 | ID=MS.gene97457.t1.exon1;Parent=MS.gene97457.t1 |
| 50664 | CDS | 9515 | 9754 | 9515 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 10241 | 10469 | 10241 | ID=MS.gene97457.t1.exon2;Parent=MS.gene97457.t1 |
| 50664 | CDS | 10241 | 10469 | 10241 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 13377 | 13471 | 13377 | ID=MS.gene97457.t1.exon3;Parent=MS.gene97457.t1 |
| 50664 | CDS | 13377 | 13471 | 13377 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 14216 | 14273 | 14216 | ID=MS.gene97457.t1.exon4;Parent=MS.gene97457.t1 |
| 50664 | CDS | 14216 | 14273 | 14216 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 14656 | 14850 | 14656 | ID=MS.gene97457.t1.exon5;Parent=MS.gene97457.t1 |
| 50664 | CDS | 14656 | 14850 | 14656 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 15038 | 15114 | 15038 | ID=MS.gene97457.t1.exon6;Parent=MS.gene97457.t1 |
| 50664 | CDS | 15038 | 15114 | 15038 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 15678 | 15760 | 15678 | ID=MS.gene97457.t1.exon7;Parent=MS.gene97457.t1 |
| 50664 | CDS | 15678 | 15760 | 15678 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 15850 | 15901 | 15850 | ID=MS.gene97457.t1.exon8;Parent=MS.gene97457.t1 |
| 50664 | CDS | 15850 | 15901 | 15850 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 16294 | 16362 | 16294 | ID=MS.gene97457.t1.exon9;Parent=MS.gene97457.t1 |
| 50664 | CDS | 16294 | 16362 | 16294 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 16493 | 16579 | 16493 | ID=MS.gene97457.t1.exon10;Parent=MS.gene97457.t1 |
| 50664 | CDS | 16493 | 16579 | 16493 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 16691 | 16752 | 16691 | ID=MS.gene97457.t1.exon11;Parent=MS.gene97457.t1 |
| 50664 | CDS | 16691 | 16752 | 16691 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| 50664 | exon | 17176 | 17311 | 17176 | ID=MS.gene97457.t1.exon12;Parent=MS.gene97457.t1 |
| 50664 | CDS | 17176 | 17311 | 17176 | ID=cds.MS.gene97457.t1;Parent=MS.gene97457.t1 |
| Gene Sequence |
| Protein sequence |
