AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048216.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048216.01.T01 MTR_1g088450 99.160 119 1 0 1 119 1 119 1.89e-83 239
MsG0080048216.01.T01 MTR_1g083430 89.916 119 11 1 2 119 7 125 4.25e-64 191
MsG0080048216.01.T01 MTR_7g112770 95.370 108 5 0 12 119 17 124 3.57e-62 186
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048216.01.T01 AT3G05560 88.793 116 13 0 4 119 9 124 1.61e-63 189
MsG0080048216.01.T01 AT3G05560 88.793 116 13 0 4 119 9 124 1.61e-63 189
MsG0080048216.01.T01 AT3G05560 88.793 116 13 0 4 119 9 124 1.61e-63 189
MsG0080048216.01.T01 AT5G27770 86.735 98 13 0 11 108 16 113 1.16e-49 154
MsG0080048216.01.T01 AT1G02830 75.229 109 27 0 2 110 9 117 8.56e-46 144

Find 38 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTTCAGGAGAGAATTA+AGG 0.293634 contig245end:+22537 MsG0080048216.01.T01:CDS
ATAAACATCTACCAACTAAA+AGG 0.310288 contig245end:-24001 None:intergenic
TTCCCTCGAGAAGTTTCTTC+AGG 0.314117 contig245end:+22525 MsG0080048216.01.T01:CDS
GCCAGTTGAGGATAAGATTA+TGG 0.328511 contig245end:+22495 MsG0080048216.01.T01:CDS
GGTGGTAAGGCTGGTGCTCT+TGG 0.362800 contig245end:+22562 MsG0080048216.01.T01:CDS
GTCTTGAGCCATTCTCCATT+AGG 0.366598 contig245end:+23802 MsG0080048216.01.T01:three_prime_UTR
ATAGATATTGCGGTCCTTGT+TGG 0.377160 contig245end:-23714 None:intergenic
TCCACAGCAATTACACCTAA+TGG 0.379407 contig245end:-23817 None:intergenic
GAGAATTAAGGTTGGTGGTA+AGG 0.387795 contig245end:+22549 MsG0080048216.01.T01:CDS
AACTTGCTGAGGGAAATAAT+TGG 0.395785 contig245end:-23909 None:intergenic
TCCATAATCTTATCCTCAAC+TGG 0.404476 contig245end:-22496 None:intergenic
CGGAGTGAAGGGTAAGAAGA+AGG 0.409364 contig245end:+22444 MsG0080048216.01.T01:CDS
ATCGGAGGTAACAGTGATCT+TGG 0.422181 contig245end:-22614 None:intergenic
CGCGAAAATGAGCGGAGTGA+AGG 0.462475 contig245end:+22432 MsG0080048216.01.T01:exon
AAAAGCACAATGTGAGGGAT+TGG 0.474548 contig245end:+23675 MsG0080048216.01.T01:CDS
CTTCAACATTGCTGAGAATG+AGG 0.480373 contig245end:+23748 MsG0080048216.01.T01:CDS
ATTAAGGTTGGTGGTAAGGC+TGG 0.484881 contig245end:+22553 MsG0080048216.01.T01:CDS
CAGTTACTCAAATGCTGGCA+TGG 0.499903 contig245end:+23970 MsG0080048216.01.T01:three_prime_UTR
CAGGAGAGAATTAAGGTTGG+TGG 0.507782 contig245end:+22544 MsG0080048216.01.T01:CDS
GCGAAAATGAGCGGAGTGAA+GGG 0.509201 contig245end:+22433 MsG0080048216.01.T01:exon
AGATTCAGTTACTCAAATGC+TGG 0.531438 contig245end:+23965 MsG0080048216.01.T01:three_prime_UTR
AATAACTATAAAACTTGCTG+AGG 0.533693 contig245end:-23920 None:intergenic
TGCTGAGAATGAGGGTGAAG+AGG 0.536820 contig245end:+23757 MsG0080048216.01.T01:CDS
TCCATTAGGTGTAATTGCTG+TGG 0.546419 contig245end:+23816 MsG0080048216.01.T01:three_prime_UTR
CGATTGCGCGAAGCCAGTTG+AGG 0.561050 contig245end:+22483 MsG0080048216.01.T01:CDS
CAATTACACCTAATGGAGAA+TGG 0.570342 contig245end:-23810 None:intergenic
GGAGTGAAGGGTAAGAAGAA+GGG 0.572006 contig245end:+22445 MsG0080048216.01.T01:CDS
CTTCAGGAGAGAATTAAGGT+TGG 0.582585 contig245end:+22541 MsG0080048216.01.T01:CDS
ATCTCAACTCATAGATATTG+CGG 0.582807 contig245end:-23724 None:intergenic
AGTCTCAACGCGAAAATGAG+CGG 0.596462 contig245end:+22424 MsG0080048216.01.T01:exon
TTTACATCACCAGGAAGAAG+GGG 0.601482 contig245end:+22397 None:intergenic
TCGTGTTATTGCATCCAACA+AGG 0.613064 contig245end:+23700 MsG0080048216.01.T01:CDS
TTCAACATTGCTGAGAATGA+GGG 0.613248 contig245end:+23749 MsG0080048216.01.T01:CDS
CTCCTGAAGAAACTTCTCGA+GGG 0.621359 contig245end:-22527 None:intergenic
ATAACTATAAAACTTGCTGA+GGG 0.626028 contig245end:-23919 None:intergenic
TCTCCTGAAGAAACTTCTCG+AGG 0.651285 contig245end:-22528 None:intergenic
TTACATCACCAGGAAGAAGG+GGG 0.678294 contig245end:+22398 None:intergenic
TTTGTCACGAGTAACAGTAA+CGG 0.687075 contig245end:-22590 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTAATTTATTATGATTC+TGG + contig245end:23424-23443 MsG0080048216.01.T01:intron 10.0%
!!! TCAAATTTTATTTAACATAA+AGG - contig245end:23402-23421 None:intergenic 10.0%
!!! GCAAGTTTTATAGTTATTAT+TGG + contig245end:23925-23944 MsG0080048216.01.T01:three_prime_UTR 20.0%
! AAAGGAATACTAAACAATAG+TGG - contig245end:23384-23403 None:intergenic 25.0%
! ATAAACATCTACCAACTAAA+AGG - contig245end:24004-24023 None:intergenic 25.0%
! CATGTGAACATTTCATTTAA+AGG - contig245end:22744-22763 None:intergenic 25.0%
!! AAAATTTTGTGTGCTTACTT+TGG + contig245end:23884-23903 MsG0080048216.01.T01:three_prime_UTR 25.0%
!! GTTTCTTTTTATAACTATGC+TGG + contig245end:22901-22920 MsG0080048216.01.T01:intron 25.0%
!! TTAAATTACTTGGTAGTAAC+TGG + contig245end:22930-22949 MsG0080048216.01.T01:intron 25.0%
!!! AATAACTATAAAACTTGCTG+AGG - contig245end:23923-23942 None:intergenic 25.0%
!!! ATAACTATAAAACTTGCTGA+GGG - contig245end:23922-23941 None:intergenic 25.0%
!!! TTTGATTTTTGAGTTACTGT+TGG + contig245end:22816-22835 MsG0080048216.01.T01:intron 25.0%
ATCTCAACTCATAGATATTG+CGG - contig245end:23727-23746 None:intergenic 30.0%
CATGTTATTGAGTAATTCTG+TGG + contig245end:22760-22779 MsG0080048216.01.T01:intron 30.0%
CTGGTAACTGTTAAATTACT+TGG + contig245end:22920-22939 MsG0080048216.01.T01:intron 30.0%
CTTAATGTTACTGATGTGTT+AGG + contig245end:22680-22699 MsG0080048216.01.T01:intron 30.0%
GGACAAAAATAATCACATGA+AGG - contig245end:23601-23620 None:intergenic 30.0%
TCAAAATAAACTGAGACACA+AGG - contig245end:23547-23566 None:intergenic 30.0%
TGAATACAAGTTGTCTTCTT+TGG + contig245end:23564-23583 MsG0080048216.01.T01:intron 30.0%
TTGTCATGAGAATCTTAAGT+TGG - contig245end:23302-23321 None:intergenic 30.0%
! TTGTTAACTTTTCTACCTTG+AGG + contig245end:23174-23193 MsG0080048216.01.T01:intron 30.0%
!! CCTTTTGGAAAAATTGCTAT+CGG - contig245end:22635-22654 None:intergenic 30.0%
!!! GTTTTTTGTCAACTTGTTAC+TGG + contig245end:23855-23874 MsG0080048216.01.T01:three_prime_UTR 30.0%
AAAAACAGTTAAACTGAGCC+AGG - contig245end:23475-23494 None:intergenic 35.0%
AAATCTCGTAGAAGATCCAA+AGG + contig245end:23011-23030 MsG0080048216.01.T01:intron 35.0%
AAGAAAACATCACAAGCCAA+AGG - contig245end:23267-23286 None:intergenic 35.0%
AATCTCGTAGAAGATCCAAA+GGG + contig245end:23012-23031 MsG0080048216.01.T01:intron 35.0%
AGATTCAGTTACTCAAATGC+TGG + contig245end:23965-23984 MsG0080048216.01.T01:three_prime_UTR 35.0%
CAATTACACCTAATGGAGAA+TGG - contig245end:23813-23832 None:intergenic 35.0%
GCAGTGAAATTTCAGAATCA+CGG - contig245end:23066-23085 None:intergenic 35.0%
GTTTCTTCAGGAGAGAATTA+AGG + contig245end:22537-22556 MsG0080048216.01.T01:CDS 35.0%
TCCATAATCTTATCCTCAAC+TGG - contig245end:22499-22518 None:intergenic 35.0%
TTAGTGTATGTTCTGATGAG+TGG + contig245end:22977-22996 MsG0080048216.01.T01:intron 35.0%
TTCAACATTGCTGAGAATGA+GGG + contig245end:23749-23768 MsG0080048216.01.T01:CDS 35.0%
TTTGGAAAAATTGCTATCGG+AGG - contig245end:22632-22651 None:intergenic 35.0%
TTTGTCACGAGTAACAGTAA+CGG - contig245end:22593-22612 None:intergenic 35.0%
! AAGACGAAGAAGTTACCTTT+TGG - contig245end:22650-22669 None:intergenic 35.0%
! ACTTGAAAAAGCACAATGTG+AGG + contig245end:23669-23688 MsG0080048216.01.T01:CDS 35.0%
! ATGTTTTGCCAAAATTGAGG+TGG + contig245end:23036-23055 MsG0080048216.01.T01:intron 35.0%
! CCGATAGCAATTTTTCCAAA+AGG + contig245end:22632-22651 MsG0080048216.01.T01:CDS 35.0%
! CTTGAAAAAGCACAATGTGA+GGG + contig245end:23670-23689 MsG0080048216.01.T01:CDS 35.0%
! GGTATGTTTTGCCAAAATTG+AGG + contig245end:23033-23052 MsG0080048216.01.T01:intron 35.0%
! TTTTGGCAAAACATACCCTT+TGG - contig245end:23030-23049 None:intergenic 35.0%
!! AACTTGCTGAGGGAAATAAT+TGG - contig245end:23912-23931 None:intergenic 35.0%
!! ATTCTGTGGCTTTGAAATGT+TGG + contig245end:22774-22793 MsG0080048216.01.T01:intron 35.0%
!!! GTGCTTTTTCAAGTACTTCT+TGG - contig245end:23663-23682 None:intergenic 35.0%
ACACACATCAGTTGCATGTT+TGG - contig245end:23093-23112 None:intergenic 40.0%
ACTGAACTTGATTCTTGTCC+TGG + contig245end:23454-23473 MsG0080048216.01.T01:intron 40.0%
ATAGATATTGCGGTCCTTGT+TGG - contig245end:23717-23736 None:intergenic 40.0%
CATCAGTTGCATGTTTGGAT+TGG - contig245end:23088-23107 None:intergenic 40.0%
CTTCAACATTGCTGAGAATG+AGG + contig245end:23748-23767 MsG0080048216.01.T01:CDS 40.0%
CTTCAGGAGAGAATTAAGGT+TGG + contig245end:22541-22560 MsG0080048216.01.T01:CDS 40.0%
GCCAGTTGAGGATAAGATTA+TGG + contig245end:22495-22514 MsG0080048216.01.T01:CDS 40.0%
GGTCAAGTACTTCAAATACC+TGG - contig245end:23642-23661 None:intergenic 40.0%
GTACTTCAAATACCTGGCAA+AGG - contig245end:23636-23655 None:intergenic 40.0%
TAATCAACACTACCTTTGCC+AGG + contig245end:23621-23640 MsG0080048216.01.T01:intron 40.0%
TCCACAGCAATTACACCTAA+TGG - contig245end:23820-23839 None:intergenic 40.0%
TCCATTAGGTGTAATTGCTG+TGG + contig245end:23816-23835 MsG0080048216.01.T01:three_prime_UTR 40.0%
! AAAAGCACAATGTGAGGGAT+TGG + contig245end:23675-23694 MsG0080048216.01.T01:CDS 40.0%
! TCGTGTTATTGCATCCAACA+AGG + contig245end:23700-23719 MsG0080048216.01.T01:CDS 40.0%
! TGGTTTCTGCACCTTTTAGT+TGG + contig245end:23990-24009 MsG0080048216.01.T01:three_prime_UTR 40.0%
!! ACTAAACAATAGTGGACACC+AGG - contig245end:23376-23395 None:intergenic 40.0%
!! GAGAATTAAGGTTGGTGGTA+AGG + contig245end:22549-22568 MsG0080048216.01.T01:CDS 40.0%
!! GCACTATGCTTGTTGTGAAA+GGG + contig245end:23218-23237 MsG0080048216.01.T01:intron 40.0%
!! GTGGCTTTGAAATGTTGGAT+GGG + contig245end:22779-22798 MsG0080048216.01.T01:intron 40.0%
!! TGGCAAAGGTAGTGTTGATT+AGG - contig245end:23622-23641 None:intergenic 40.0%
!! TGGCTTTGAAATGTTGGATG+GGG + contig245end:22780-22799 MsG0080048216.01.T01:intron 40.0%
!! TGTGGCTTTGAAATGTTGGA+TGG + contig245end:22778-22797 MsG0080048216.01.T01:intron 40.0%
AAAATGCCATTCCCAGCCTT+TGG + contig245end:23248-23267 MsG0080048216.01.T01:intron 45.0%
AAACATCACAAGCCAAAGGC+TGG - contig245end:23263-23282 None:intergenic 45.0%
AACATCACAAGCCAAAGGCT+GGG - contig245end:23262-23281 None:intergenic 45.0%
AGGTCTAGTTGTCGCTGTTT+GGG + contig245end:23194-23213 MsG0080048216.01.T01:intron 45.0%
AGTCTCAACGCGAAAATGAG+CGG + contig245end:22424-22443 MsG0080048216.01.T01:exon 45.0%
ATCGGAGGTAACAGTGATCT+TGG - contig245end:22617-22636 None:intergenic 45.0%
CAGGAGAGAATTAAGGTTGG+TGG + contig245end:22544-22563 MsG0080048216.01.T01:CDS 45.0%
CAGTTACTCAAATGCTGGCA+TGG + contig245end:23970-23989 MsG0080048216.01.T01:three_prime_UTR 45.0%
CTCCTGAAGAAACTTCTCGA+GGG - contig245end:22530-22549 None:intergenic 45.0%
GGAGTGAAGGGTAAGAAGAA+GGG + contig245end:22445-22464 MsG0080048216.01.T01:CDS 45.0%
GTCTTGAGCCATTCTCCATT+AGG + contig245end:23802-23821 MsG0080048216.01.T01:three_prime_UTR 45.0%
TCTCCTGAAGAAACTTCTCG+AGG - contig245end:22531-22550 None:intergenic 45.0%
TTCCCTCGAGAAGTTTCTTC+AGG + contig245end:22525-22544 MsG0080048216.01.T01:CDS 45.0%
! GTGATGTTTTCTTGCATCCG+CGG + contig245end:23274-23293 MsG0080048216.01.T01:intron 45.0%
!! AGAATCTTAAGTTGGCACCG+CGG - contig245end:23294-23313 None:intergenic 45.0%
!! ATTAAGGTTGGTGGTAAGGC+TGG + contig245end:22553-22572 MsG0080048216.01.T01:CDS 45.0%
!! CGCACTATGCTTGTTGTGAA+AGG + contig245end:23217-23236 MsG0080048216.01.T01:intron 45.0%
ACAGCGACAACTAGACCTCA+AGG - contig245end:23192-23211 None:intergenic 50.0%
CGGAGTGAAGGGTAAGAAGA+AGG + contig245end:22444-22463 MsG0080048216.01.T01:CDS 50.0%
GAGGTCTAGTTGTCGCTGTT+TGG + contig245end:23193-23212 MsG0080048216.01.T01:intron 50.0%
GCGAAAATGAGCGGAGTGAA+GGG + contig245end:22433-22452 MsG0080048216.01.T01:exon 50.0%
GCTCATGTTCCTGCATTTCC+TGG + contig245end:23355-23374 MsG0080048216.01.T01:intron 50.0%
TGCTGAGAATGAGGGTGAAG+AGG + contig245end:23757-23776 MsG0080048216.01.T01:CDS 50.0%
! ACGGTGAGCCACCTCAATTT+TGG - contig245end:23047-23066 None:intergenic 50.0%
! TAGTGGACACCAGGAAATGC+AGG - contig245end:23367-23386 None:intergenic 50.0%
AGACTTCACCCCCTTCTTCC+TGG - contig245end:22409-22428 None:intergenic 55.0%
CACAAGCCAAAGGCTGGGAA+TGG - contig245end:23257-23276 None:intergenic 55.0%
CGCGAAAATGAGCGGAGTGA+AGG + contig245end:22432-22451 MsG0080048216.01.T01:exon 55.0%
CGATTGCGCGAAGCCAGTTG+AGG + contig245end:22483-22502 MsG0080048216.01.T01:CDS 60.0%
GGTGGTAAGGCTGGTGCTCT+TGG + contig245end:22562-22581 MsG0080048216.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
contig245end gene 22402 24039 22402 ID=MsG0080048216.01;Name=MsG0080048216.01
contig245end mRNA 22402 24039 22402 ID=MsG0080048216.01.T01;Parent=MsG0080048216.01;Name=MsG0080048216.01.T01;_AED=0.45;_eAED=0.45;_QI=37|1|1|1|1|1|2|252|119
contig245end exon 23643 24039 23643 ID=MsG0080048216.01.T01:exon:22934;Parent=MsG0080048216.01.T01
contig245end exon 22402 22653 22402 ID=MsG0080048216.01.T01:exon:22933;Parent=MsG0080048216.01.T01
contig245end five_prime_UTR 22402 22438 22402 ID=MsG0080048216.01.T01:five_prime_utr;Parent=MsG0080048216.01.T01
contig245end CDS 22439 22653 22439 ID=MsG0080048216.01.T01:cds;Parent=MsG0080048216.01.T01
contig245end CDS 23643 23787 23643 ID=MsG0080048216.01.T01:cds;Parent=MsG0080048216.01.T01
contig245end three_prime_UTR 23788 24039 23788 ID=MsG0080048216.01.T01:three_prime_utr;Parent=MsG0080048216.01.T01
Gene Sequence

>MsG0080048216.01.T01

ATGAGCGGAGTGAAGGGTAAGAAGAAGGGAACAAGCTTCGTGATCGATTGCGCGAAGCCAGTTGAGGATAAGATTATGGACATCGCTTCCCTCGAGAAGTTTCTTCAGGAGAGAATTAAGGTTGGTGGTAAGGCTGGTGCTCTTGGTGATTCCGTTACTGTTACTCGTGACAAATCCAAGATCACTGTTACCTCCGATAGCAATTTTTCCAAAAGGTATTTGAAGTACTTGACCAAGAAGTACTTGAAAAAGCACAATGTGAGGGATTGGCTTCGTGTTATTGCATCCAACAAGGACCGCAATATCTATGAGTTGAGATACTTCAACATTGCTGAGAATGAGGGTGAAGAGGAAGACTAA

Protein sequence

>MsG0080048216.01.T01

MSGVKGKKKGTSFVIDCAKPVEDKIMDIASLEKFLQERIKVGGKAGALGDSVTVTRDKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNIYELRYFNIAENEGEEED*