AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000042.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000042.01.T01 MTR_1g025610 97.857 140 3 0 2 141 29 168 8.40e-93 285
MsG0180000042.01.T01 MTR_5g022610 82.609 138 24 0 2 139 29 166 3.15e-80 249
MsG0180000042.01.T01 MTR_5g022610 82.609 138 24 0 2 139 29 166 3.36e-79 249
MsG0180000042.01.T01 MTR_1g025610 99.038 104 1 0 38 141 1 104 7.24e-68 218
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000042.01.T01 AT2G17790 77.536 138 31 0 2 139 29 166 1.75e-76 242
MsG0180000042.01.T01 AT1G75850 81.159 138 26 0 2 139 30 167 8.70e-76 240
MsG0180000042.01.T01 AT3G51310 74.638 138 35 0 2 139 26 163 6.42e-70 224

Find 22 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTAAGAACGTCCCTTAC+AGG 0.258965 1:-707719 None:intergenic
CAGGAGTATCCTTACATCTA+AGG 0.365487 1:-707700 None:intergenic
TTGAATTGAATTGCGTTTGC+AGG 0.382991 1:+706276 None:intergenic
AGAGACAAGTTGCCTGATAT+TGG 0.383749 1:+707818 MsG0180000042.01.T01:CDS
TCGTATTCAGAGCCAATATC+AGG 0.386867 1:-707830 None:intergenic
GCGAAAACCACTTACAATCT+AGG 0.393011 1:-707070 None:intergenic
CACAGTTCATAATATTTATG+AGG 0.433658 1:-706373 None:intergenic
ATCTGTCTACCTTAGATGTA+AGG 0.462531 1:+707691 MsG0180000042.01.T01:CDS
TATGAGCTTGTTCAACATGC+TGG 0.491414 1:+707037 MsG0180000042.01.T01:CDS
GATGTAAGGATACTCCTGTA+AGG 0.494334 1:+707705 MsG0180000042.01.T01:CDS
AGGTATCTTCTTTGCACGGT+TGG 0.502581 1:+707668 MsG0180000042.01.T01:intron
TGCCAGGTATCTTCTTTGCA+CGG 0.522853 1:+707664 MsG0180000042.01.T01:intron
GGTGTTCAGCATCCGATTCG+TGG 0.538893 1:+707761 MsG0180000042.01.T01:CDS
TTGGCTCTGAATACGAGGAT+AGG 0.591352 1:+707837 MsG0180000042.01.T01:CDS
ATGAGCTTGTTCAACATGCT+GGG 0.600118 1:+707038 MsG0180000042.01.T01:CDS
ATGTAAGGATACTCCTGTAA+GGG 0.600906 1:+707706 MsG0180000042.01.T01:CDS
GGACGTTCTTAAAGACCTAG+TGG 0.614680 1:+707727 MsG0180000042.01.T01:CDS
CACCGCGACACATTTCCACT+AGG 0.615529 1:-707742 None:intergenic
TTTAAAGATGAAAGTAGACA+TGG 0.632800 1:+706995 MsG0180000042.01.T01:CDS
TGATATTGGCTCTGAATACG+AGG 0.659487 1:+707832 MsG0180000042.01.T01:CDS
GACCTAGTGGAAATGTGTCG+CGG 0.671661 1:+707740 MsG0180000042.01.T01:CDS
TCTGAATACGAGGATAGGTG+AGG 0.699806 1:+707842 MsG0180000042.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTATATATTTCATTGATA+TGG + Chr1:707310-707329 MsG0180000042.01.T01:intron 15.0%
!!! ATTTTTGCTTATGTTATATA+AGG + Chr1:706475-706494 MsG0180000042.01.T01:intron 15.0%
! AGAAGCTAGAACTAAAATAA+AGG + Chr1:707278-707297 MsG0180000042.01.T01:intron 25.0%
! ATAAGTACAACTACAAGAAA+TGG - Chr1:707123-707142 None:intergenic 25.0%
! CACAGTTCATAATATTTATG+AGG - Chr1:706376-706395 None:intergenic 25.0%
! CATAATTGAATGTAAATCAC+AGG - Chr1:706532-706551 None:intergenic 25.0%
! TACCAAATTAGCTACATAAT+AGG + Chr1:707530-707549 MsG0180000042.01.T01:intron 25.0%
! TTTAAAGATGAAAGTAGACA+TGG + Chr1:706995-707014 MsG0180000042.01.T01:CDS 25.0%
CATTGTATGGTCTCATAATT+TGG - Chr1:707449-707468 None:intergenic 30.0%
TATATTGCCTAGATTGTAAG+TGG + Chr1:707063-707082 MsG0180000042.01.T01:intron 30.0%
TGATTTCGATTATAGTCATG+CGG + Chr1:707162-707181 MsG0180000042.01.T01:intron 30.0%
! ACAAATTTTATCGCACTAAG+TGG - Chr1:707414-707433 None:intergenic 30.0%
! ATTGTTGTTAATTTACTGCC+AGG + Chr1:707648-707667 MsG0180000042.01.T01:intron 30.0%
! CAAATTTTATCGCACTAAGT+GGG - Chr1:707413-707432 None:intergenic 30.0%
! CAGCTTATATAAGCATTGTA+TGG - Chr1:707462-707481 None:intergenic 30.0%
! GCTTCTCTTATAGTTTTTCT+AGG - Chr1:707361-707380 None:intergenic 30.0%
! TTACTCTCAAAGGATCTTTT+AGG - Chr1:707556-707575 None:intergenic 30.0%
! TTGTTGAGTCGTTATTCTTT+TGG + Chr1:707578-707597 MsG0180000042.01.T01:intron 30.0%
AAGGATTCTACCTATCATCA+TGG + Chr1:707605-707624 MsG0180000042.01.T01:intron 35.0%
ATCTGTCTACCTTAGATGTA+AGG + Chr1:707691-707710 MsG0180000042.01.T01:CDS 35.0%
ATGTAAGGATACTCCTGTAA+GGG + Chr1:707706-707725 MsG0180000042.01.T01:CDS 35.0%
CTCAAAACCCAATTCAAGTA+GGG + Chr1:707233-707252 MsG0180000042.01.T01:intron 35.0%
CTCAACAAGTTTACTCTCAA+AGG - Chr1:707566-707585 None:intergenic 35.0%
GGCCTATTATGTAGCTAATT+TGG - Chr1:707535-707554 None:intergenic 35.0%
GTCATGCGGTTATTTATGAA+TGG + Chr1:707176-707195 MsG0180000042.01.T01:intron 35.0%
TCATTCAGAACCATGATGAT+AGG - Chr1:707618-707637 None:intergenic 35.0%
TCTCAAAACCCAATTCAAGT+AGG + Chr1:707232-707251 MsG0180000042.01.T01:intron 35.0%
TTCAGCTGTTCATGTCTATT+TGG + Chr1:706909-706928 MsG0180000042.01.T01:intron 35.0%
TTCCTCTACCATTAGGTTTA+GGG - Chr1:707336-707355 None:intergenic 35.0%
TTGAACGTAATCACAAGTGT+TGG - Chr1:706657-706676 None:intergenic 35.0%
! TCGTTATTCTTTTGGTCTCA+AGG + Chr1:707586-707605 MsG0180000042.01.T01:intron 35.0%
! TGAGAGCTTTTGATGAGTTA+CGG + Chr1:706957-706976 MsG0180000042.01.T01:CDS 35.0%
! TTGGTAACACTACTATTGTG+TGG - Chr1:707516-707535 None:intergenic 35.0%
AGAGACAAGTTGCCTGATAT+TGG + Chr1:707818-707837 MsG0180000042.01.T01:CDS 40.0%
AGTGTGACCCTACTTGAATT+GGG - Chr1:707243-707262 None:intergenic 40.0%
CTCAAAAACAGACCACGAAT+CGG - Chr1:707776-707795 None:intergenic 40.0%
CTTCCTCTACCATTAGGTTT+AGG - Chr1:707337-707356 None:intergenic 40.0%
GATGTAAGGATACTCCTGTA+AGG + Chr1:707705-707724 MsG0180000042.01.T01:CDS 40.0%
GCGAAAACCACTTACAATCT+AGG - Chr1:707073-707092 None:intergenic 40.0%
GTTGCACTTTCACTTGTGAT+AGG + Chr1:706612-706631 MsG0180000042.01.T01:intron 40.0%
TAGCTTCTGTCTCTGAATTC+TGG - Chr1:707266-707285 None:intergenic 40.0%
TATGATGAGTGTCAGTGTGT+TGG + Chr1:706696-706715 MsG0180000042.01.T01:intron 40.0%
TCGTATTCAGAGCCAATATC+AGG - Chr1:707833-707852 None:intergenic 40.0%
TGATATGGCCCTAAACCTAA+TGG + Chr1:707325-707344 MsG0180000042.01.T01:intron 40.0%
! ATGAGCTTGTTCAACATGCT+GGG + Chr1:707038-707057 MsG0180000042.01.T01:CDS 40.0%
! GTATGTGTTGGTGCTTCATA+GGG + Chr1:706881-706900 MsG0180000042.01.T01:intron 40.0%
! TATGAGCTTGTTCAACATGC+TGG + Chr1:707037-707056 MsG0180000042.01.T01:CDS 40.0%
! TCTTTAAGAACGTCCCTTAC+AGG - Chr1:707722-707741 None:intergenic 40.0%
! TGTATGTGTTGGTGCTTCAT+AGG + Chr1:706880-706899 MsG0180000042.01.T01:intron 40.0%
! TTTTGATGAGTTACGGAGGT+TGG + Chr1:706964-706983 MsG0180000042.01.T01:CDS 40.0%
!! AACGTAATCACAAGTGTTGG+TGG - Chr1:706654-706673 None:intergenic 40.0%
!! CAGGAGTATCCTTACATCTA+AGG - Chr1:707703-707722 None:intergenic 40.0%
!! TGATATTGGCTCTGAATACG+AGG + Chr1:707832-707851 MsG0180000042.01.T01:CDS 40.0%
AACCGTGCAAAGAAGATACC+TGG - Chr1:707669-707688 None:intergenic 45.0%
AGGTATCTTCTTTGCACGGT+TGG + Chr1:707668-707687 MsG0180000042.01.T01:intron 45.0%
CAACATGTCAGTGTCGTGTT+TGG + Chr1:706857-706876 MsG0180000042.01.T01:intron 45.0%
CTAGGTCTTCCTCTACCATT+AGG - Chr1:707343-707362 None:intergenic 45.0%
GAGTGTGACCCTACTTGAAT+TGG - Chr1:707244-707263 None:intergenic 45.0%
GCACTTTCACTTGTGATAGG+AGG + Chr1:706615-706634 MsG0180000042.01.T01:intron 45.0%
GGACGTTCTTAAAGACCTAG+TGG + Chr1:707727-707746 MsG0180000042.01.T01:CDS 45.0%
TACTGAGACGTGACAAACAC+TGG - Chr1:706787-706806 None:intergenic 45.0%
TGCCAGGTATCTTCTTTGCA+CGG + Chr1:707664-707683 MsG0180000042.01.T01:intron 45.0%
! GAGCTTTTGATGAGTTACGG+AGG + Chr1:706960-706979 MsG0180000042.01.T01:CDS 45.0%
! TGTGTTGGTGTCTCTGTAAG+TGG + Chr1:706711-706730 MsG0180000042.01.T01:intron 45.0%
!! GTCGTGTTTGGTGTATGTGT+TGG + Chr1:706869-706888 MsG0180000042.01.T01:intron 45.0%
!! TTGGCTCTGAATACGAGGAT+AGG + Chr1:707837-707856 MsG0180000042.01.T01:CDS 45.0%
!!! CGATTCGTGGTCTGTTTTTG+AGG + Chr1:707774-707793 MsG0180000042.01.T01:CDS 45.0%
AGCGTCGTGTGTGCTTCATA+GGG + Chr1:706579-706598 MsG0180000042.01.T01:intron 50.0%
CATGTACACATGTCGGACAC+AGG - Chr1:706506-706525 None:intergenic 50.0%
CTGTGTCCGACATGTGTACA+TGG + Chr1:706504-706523 MsG0180000042.01.T01:intron 50.0%
GACCTAGTGGAAATGTGTCG+CGG + Chr1:707740-707759 MsG0180000042.01.T01:CDS 50.0%
GGCCCTAAACCTAATGGTAG+AGG + Chr1:707331-707350 MsG0180000042.01.T01:intron 50.0%
! ACACCTTCTGTCAGAAGCGT+CGG - Chr1:706762-706781 None:intergenic 50.0%
! CAGGTGCCATGTACACATGT+CGG - Chr1:706513-706532 None:intergenic 50.0%
CACCGCGACACATTTCCACT+AGG - Chr1:707745-707764 None:intergenic 55.0%
CAGCGTCGTGTGTGCTTCAT+AGG + Chr1:706578-706597 MsG0180000042.01.T01:intron 55.0%
! GGTGTTCAGCATCCGATTCG+TGG + Chr1:707761-707780 MsG0180000042.01.T01:CDS 55.0%
!! CACAAGTGTTGGTGGCGTAC+TGG - Chr1:706646-706665 None:intergenic 55.0%
! GCACCGACGCTTCTGACAGA+AGG + Chr1:706756-706775 MsG0180000042.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 706286 707862 706286 ID=MsG0180000042.01;Name=MsG0180000042.01
Chr1 mRNA 706286 707862 706286 ID=MsG0180000042.01.T01;Parent=MsG0180000042.01;Name=MsG0180000042.01.T01;_AED=0.48;_eAED=0.49;_QI=0|1|0.33|1|0.5|0.33|3|0|141
Chr1 exon 706286 706394 706286 ID=MsG0180000042.01.T01:exon:7739;Parent=MsG0180000042.01.T01
Chr1 exon 706954 707077 706954 ID=MsG0180000042.01.T01:exon:7738;Parent=MsG0180000042.01.T01
Chr1 exon 707670 707862 707670 ID=MsG0180000042.01.T01:exon:7737;Parent=MsG0180000042.01.T01
Chr1 CDS 706286 706394 706286 ID=MsG0180000042.01.T01:cds;Parent=MsG0180000042.01.T01
Chr1 CDS 706954 707077 706954 ID=MsG0180000042.01.T01:cds;Parent=MsG0180000042.01.T01
Chr1 CDS 707670 707862 707670 ID=MsG0180000042.01.T01:cds;Parent=MsG0180000042.01.T01
Gene Sequence

>MsG0180000042.01.T01

TTGCGTTTGCAGGATGATAACAATCTCAGAGATTCACTCAAATACTCAGCACAGATGCTATCTGAACTTCGAACTTCTAGACTCTCTCCTCATAAATATTATGAACTGTATATGAGAGCTTTTGATGAGTTACGGAGGTTGGAGATGTTCTTTAAAGATGAAAGTAGACATGGTGTTTCGATTGTTGATTTGTATGAGCTTGTTCAACATGCTGGGAATATATTGCCTAGATTGTATCTTCTTTGCACGGTTGGATCTGTCTACCTTAGATGTAAGGATACTCCTGTAAGGGACGTTCTTAAAGACCTAGTGGAAATGTGTCGCGGTGTTCAGCATCCGATTCGTGGTCTGTTTTTGAGGAGTTATCTTTCTCAAGTCAGTAGAGACAAGTTGCCTGATATTGGCTCTGAATACGAGGATAGGTGA

Protein sequence

>MsG0180000042.01.T01

LRLQDDNNLRDSLKYSAQMLSELRTSRLSPHKYYELYMRAFDELRRLEMFFKDESRHGVSIVDLYELVQHAGNILPRLYLLCTVGSVYLRCKDTPVRDVLKDLVEMCRGVQHPIRGLFLRSYLSQVSRDKLPDIGSEYEDR*