AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000221.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000221.01.T01 MTR_1g021100 80.819 464 25 3 29 428 361 824 0.0 729
MsG0180000221.01.T01 MTR_1g021110 63.305 466 105 4 29 428 358 823 0.0 559
MsG0180000221.01.T01 MTR_0391s0020 28.484 488 254 12 29 426 334 816 2.50e-56 199
MsG0180000221.01.T01 MTR_8g079360 28.279 488 253 12 29 426 324 804 3.60e-56 198
MsG0180000221.01.T01 MTR_8g479430 31.183 465 251 9 29 426 349 811 7.10e-56 197
MsG0180000221.01.T01 MTR_5g018060 30.754 504 248 14 17 426 328 824 4.66e-55 196
MsG0180000221.01.T01 MTR_5g018070 33.168 404 221 10 30 390 263 660 5.61e-55 194
MsG0180000221.01.T01 MTR_5g018120 34.439 392 218 12 30 389 329 713 7.99e-55 195
MsG0180000221.01.T01 MTR_8g079400 27.926 487 256 12 29 426 300 780 9.12e-53 189
MsG0180000221.01.T01 MTR_8g079400 27.926 487 256 12 29 426 310 790 1.38e-52 188
MsG0180000221.01.T01 MTR_8g079450 28.279 488 253 13 29 426 326 806 3.91e-52 187
MsG0180000221.01.T01 MTR_5g018210 30.303 495 242 14 30 426 342 831 1.58e-50 183
MsG0180000221.01.T01 MTR_8g079280 29.102 512 256 15 8 426 256 753 1.41e-49 179
MsG0180000221.01.T01 MTR_8g479450 30.258 466 256 9 29 427 356 819 1.18e-48 177
MsG0180000221.01.T01 MTR_8g079520 32.741 394 224 12 29 392 336 718 1.89e-47 174
MsG0180000221.01.T01 MTR_8g079550 29.095 464 245 13 29 418 331 784 2.12e-45 168
MsG0180000221.01.T01 MTR_8g079380 26.237 465 248 13 51 426 109 567 1.43e-44 163
MsG0180000221.01.T01 MTR_5g018160 37.500 176 90 6 31 201 225 385 5.48e-24 103
MsG0180000221.01.T01 MTR_8g079350 41.880 117 63 3 29 141 287 402 7.42e-20 91.7
MsG0180000221.01.T01 MTR_8g079355 25.784 287 149 6 202 426 14 298 1.16e-16 80.5
MsG0180000221.01.T01 MTR_7g024973 33.981 103 67 1 314 416 20 121 7.94e-16 74.3
MsG0180000221.01.T01 MTR_4g022480 23.464 358 221 10 29 368 348 670 4.98e-14 74.7
MsG0180000221.01.T01 MTR_4g021690 24.090 357 222 13 29 368 348 672 1.31e-13 73.6
MsG0180000221.01.T01 MTR_4g022460 22.667 375 244 12 8 368 329 671 1.18e-12 70.5
MsG0180000221.01.T01 MTR_6g052455 26.149 348 206 12 29 368 238 542 3.18e-12 69.3
MsG0180000221.01.T01 MTR_7g088290 25.568 352 220 11 30 371 342 661 2.72e-11 66.2
MsG0180000221.01.T01 MTR_3g466750 24.302 358 211 16 27 361 312 632 2.85e-11 66.2
MsG0180000221.01.T01 MTR_0511s0020 25.287 348 192 12 29 368 347 634 7.28e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000221.01.T01 AT4G33300 47.854 466 176 5 29 428 352 816 8.52e-141 422
MsG0180000221.01.T01 AT4G33300 47.854 466 176 5 29 428 352 816 8.52e-141 422
MsG0180000221.01.T01 AT5G04720 42.489 466 201 5 29 428 347 811 2.68e-126 385
MsG0180000221.01.T01 AT1G33560 42.979 470 202 5 24 428 319 787 8.51e-123 375
MsG0180000221.01.T01 AT5G47280 41.416 466 206 5 29 428 159 623 2.01e-120 364
MsG0180000221.01.T01 AT5G66910 28.750 480 259 12 20 426 343 812 7.56e-49 178
MsG0180000221.01.T01 AT5G66900 28.571 469 254 13 20 418 341 798 4.95e-46 170
MsG0180000221.01.T01 AT5G66900 28.571 469 254 13 20 418 389 846 1.03e-45 169
MsG0180000221.01.T01 AT5G66890 28.607 402 206 12 85 414 8 400 1.24e-33 130
MsG0180000221.01.T01 AT5G66900 30.693 202 128 5 20 218 389 581 6.28e-20 92.8
MsG0180000221.01.T01 AT3G50950 24.438 356 208 14 29 366 349 661 7.67e-15 77.4
MsG0180000221.01.T01 AT3G50950 24.438 356 208 14 29 366 349 661 7.67e-15 77.4

Find 67 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTATTAGAGCTCTCAAATT+TGG 0.198230 1:+3037583 None:intergenic
TTCAATGAAATAGCAGATTT+TGG 0.204325 1:+3037013 None:intergenic
GAGATCAACCTGAAATGTTT+TGG 0.231922 1:-3038412 MsG0180000221.01.T01:CDS
TTGTGTCTCAACCACTCTTT+TGG 0.235777 1:+3037864 None:intergenic
ACACTTTCCCAAAACATTTC+AGG 0.255125 1:+3038404 None:intergenic
AAAGTTTCCATCCCTCAATT+TGG 0.261702 1:-3037476 MsG0180000221.01.T01:CDS
GATCATAAATTTCACATCTA+AGG 0.263048 1:-3037636 MsG0180000221.01.T01:CDS
GGGAGTCCTTTCTCTCGTTT+TGG 0.283696 1:+3037885 None:intergenic
ACTCTTTGATATCTTCCTTT+AGG 0.293617 1:+3038297 None:intergenic
CTATTTCATTGAAGTCTCTT+AGG 0.296603 1:-3037003 MsG0180000221.01.T01:CDS
CCATTGGCTCTTAAAGTGAT+TGG 0.324115 1:-3038446 MsG0180000221.01.T01:CDS
TTATTGCCATTCTTTCAATT+AGG 0.337462 1:+3038330 None:intergenic
GATACTTGAATATGAACATT+TGG 0.340202 1:+3036923 None:intergenic
ACTAGTTTACCAATCTCTTC+AGG 0.357302 1:+3037085 None:intergenic
ATGATCAGAACTTCAGCCTT+AGG 0.394151 1:+3037653 None:intergenic
TGAGGGAATGCTGTATGATT+AGG 0.402959 1:-3037042 MsG0180000221.01.T01:CDS
ATAACGGACACGCGTATAAC+CGG 0.403578 1:+3037191 None:intergenic
CATGAGATTCTCCAATACTT+TGG 0.404274 1:+3038360 None:intergenic
CATCTTTCTCATCAATATCA+TGG 0.409451 1:+3038198 None:intergenic
TTACATTGTAGGTGAAATGA+AGG 0.427172 1:-3037702 MsG0180000221.01.T01:intron
TAACAGTATACAATGGAAAC+AGG 0.440675 1:-3038492 MsG0180000221.01.T01:CDS
GAAACAATTTGAGCTTCAAA+TGG 0.444460 1:+3037834 None:intergenic
GATTGGTAAACTAGTTAGCT+TGG 0.450698 1:-3037077 MsG0180000221.01.T01:CDS
AATCAACCTAATTGAAAGAA+TGG 0.452666 1:-3038336 MsG0180000221.01.T01:CDS
TTTACAGCTTGCAAAGAAGA+TGG 0.456900 1:-3040121 MsG0180000221.01.T01:CDS
GTGTAAACTTGAATTTCCTA+AGG 0.474567 1:-3037669 MsG0180000221.01.T01:CDS
TTGAATATGAACATTTGGCT+TGG 0.475607 1:+3036928 None:intergenic
ATGAGAATGCCTCCAAATTG+AGG 0.482059 1:+3037464 None:intergenic
ATTTGTGATGAGGAAGTGTT+TGG 0.483395 1:-3036974 MsG0180000221.01.T01:CDS
GGCATTCTCATGGAAAACTT+AGG 0.485804 1:-3037452 MsG0180000221.01.T01:CDS
TAGATGCTCTCATCAATATG+TGG 0.497821 1:-3038229 MsG0180000221.01.T01:CDS
GCATTCTCATGGAAAACTTA+GGG 0.506416 1:-3037451 MsG0180000221.01.T01:CDS
GTTTCCATCCCTCAATTTGG+AGG 0.506577 1:-3037473 MsG0180000221.01.T01:CDS
TTATCTCAAGGCCAAAGTAT+TGG 0.506867 1:-3038371 MsG0180000221.01.T01:CDS
TCAATTTGGAGGCATTCTCA+TGG 0.509872 1:-3037462 MsG0180000221.01.T01:CDS
TGAGAATGCCTCCAAATTGA+GGG 0.513851 1:+3037465 None:intergenic
ATGAGGAAGTGTTTGGTATG+TGG 0.519732 1:-3036967 MsG0180000221.01.T01:CDS
TGTAGGTGAAATGAAGGAAA+GGG 0.522335 1:-3037696 MsG0180000221.01.T01:intron
GTTATGCCAAAACGAGAGAA+AGG 0.524349 1:-3037891 MsG0180000221.01.T01:CDS
CTTGGAAAAGATTGACATGA+GGG 0.548288 1:-3037059 MsG0180000221.01.T01:CDS
GTGAAATGAAGGAAAGGGAT+TGG 0.550751 1:-3037691 MsG0180000221.01.T01:CDS
TTGTAGGTGAAATGAAGGAA+AGG 0.551543 1:-3037697 MsG0180000221.01.T01:intron
CCAATCACTTTAAGAGCCAA+TGG 0.561511 1:+3038446 None:intergenic
TGGAATGGCTTAGTGAGTAA+TGG 0.561814 1:-3036883 None:intergenic
AATAAGTATCAACTACCTAA+AGG 0.566013 1:-3038312 MsG0180000221.01.T01:CDS
CGAGTCATGTCACAAACGCT+AGG 0.589205 1:+3037154 None:intergenic
AGATGCTCTCATCAATATGT+GGG 0.589960 1:-3038228 MsG0180000221.01.T01:CDS
AATTTGGCATTATCTATGAA+AGG 0.596417 1:+3037599 None:intergenic
TGGCAAACTTGAGAAGCCTG+TGG 0.604919 1:-3037505 MsG0180000221.01.T01:CDS
CAAATTGTTTCAATTCACAC+AGG 0.605954 1:-3037822 MsG0180000221.01.T01:intron
TAAGATCTCTAGAGATACTC+CGG 0.608580 1:-3037210 MsG0180000221.01.T01:CDS
ACTTGATCTATCAGTGCTGG+TGG 0.615020 1:-3038020 MsG0180000221.01.T01:intron
TAGGCTTGTAATTTGTGATG+AGG 0.615043 1:-3036984 MsG0180000221.01.T01:CDS
GTTATTCTAACAGTATACAA+TGG 0.621542 1:-3038499 MsG0180000221.01.T01:CDS
AATGCCTCCAAATTGAGGGA+TGG 0.626787 1:+3037469 None:intergenic
GCTTGGAAAAGATTGACATG+AGG 0.627984 1:-3037060 MsG0180000221.01.T01:CDS
TTGGCATTATCTATGAAAGG+AGG 0.633651 1:+3037602 None:intergenic
GATGACTCAAATTAACTGCA+AGG 0.638388 1:+3037943 None:intergenic
AGAAAGGACTCCCAAAAGAG+TGG 0.643746 1:-3037875 MsG0180000221.01.T01:CDS
TGAGTGTGAAAATCTTCCAT+TGG 0.648401 1:-3038462 MsG0180000221.01.T01:CDS
TGAAAACTGAAACATTGTGA+AGG 0.650959 1:+3037534 None:intergenic
GTCATGTCACAAACGCTAGG+AGG 0.654064 1:+3037157 None:intergenic
GTACCGTGCTTCTTTCACCA+AGG 0.672292 1:+3038133 None:intergenic
TCACCTTGGTGAAAGAAGCA+CGG 0.677428 1:-3038136 MsG0180000221.01.T01:intron
AGACTTGTGTGCATTCCCTG+AGG 0.679758 1:-3038267 MsG0180000221.01.T01:CDS
TATGTGGAAAGATGTTGAGA+AGG 0.701600 1:-3036951 MsG0180000221.01.T01:CDS
ATATTGATGAGAGCATCTAG+AGG 0.718853 1:+3038233 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAAACTTACAATAATATTT+TGG - Chr1:3037498-3037517 MsG0180000221.01.T01:CDS 15.0%
!! AATATTCACTGCAAATTATA+AGG - Chr1:3037919-3037938 MsG0180000221.01.T01:CDS 20.0%
!! CAACTTGTATTTAAATATTC+CGG + Chr1:3038356-3038375 None:intergenic 20.0%
!! TAAGATTAATAACATCTAGA+GGG - Chr1:3039616-3039635 MsG0180000221.01.T01:intron 20.0%
!!! AATAATATTTTGGAGATCTA+TGG - Chr1:3037508-3037527 MsG0180000221.01.T01:CDS 20.0%
!!! ATTTTCTTTTATTCAAGCTT+AGG + Chr1:3038391-3038410 None:intergenic 20.0%
!!! GAAATAAGTTGAAAATAGTA+TGG - Chr1:3037530-3037549 MsG0180000221.01.T01:CDS 20.0%
!!! TTGAGTAATTAACATTGTTT+TGG - Chr1:3038070-3038089 MsG0180000221.01.T01:intron 20.0%
!!! TTTTCTTTTATTCAAGCTTA+GGG + Chr1:3038390-3038409 None:intergenic 20.0%
! AAATTCTCATTAGCTGTTAA+AGG + Chr1:3038012-3038031 None:intergenic 25.0%
! AATAAGTATCAACTACCTAA+AGG - Chr1:3038712-3038731 MsG0180000221.01.T01:intron 25.0%
! AATCAACCTAATTGAAAGAA+TGG - Chr1:3038688-3038707 MsG0180000221.01.T01:intron 25.0%
! AATTCTCATTAGCTGTTAAA+GGG + Chr1:3038011-3038030 None:intergenic 25.0%
! AATTTGCAGTGAATATTGTT+GGG + Chr1:3037916-3037935 None:intergenic 25.0%
! AATTTGGCATTATCTATGAA+AGG + Chr1:3039428-3039447 None:intergenic 25.0%
! GATACTTGAATATGAACATT+TGG + Chr1:3040104-3040123 None:intergenic 25.0%
! GATCATAAATTTCACATCTA+AGG - Chr1:3039388-3039407 MsG0180000221.01.T01:intron 25.0%
! GTAAGATTAATAACATCTAG+AGG - Chr1:3039615-3039634 MsG0180000221.01.T01:intron 25.0%
! GTTATTCTAACAGTATACAA+TGG - Chr1:3038525-3038544 MsG0180000221.01.T01:intron 25.0%
! TTATTGCCATTCTTTCAATT+AGG + Chr1:3038697-3038716 None:intergenic 25.0%
! TTTAACAGCTAATGAGAATT+TGG - Chr1:3038011-3038030 MsG0180000221.01.T01:intron 25.0%
!! TAATTTGCAGTGAATATTGT+TGG + Chr1:3037917-3037936 None:intergenic 25.0%
!! TATCTATTTTCTTTTGAACC+AGG - Chr1:3038499-3038518 MsG0180000221.01.T01:CDS 25.0%
!! TGTGATGAATTTTACATTGT+AGG - Chr1:3039311-3039330 MsG0180000221.01.T01:intron 25.0%
!! TGTTTCAGTTTTCAACAATT+TGG - Chr1:3039499-3039518 MsG0180000221.01.T01:intron 25.0%
!! TTCAATGAAATAGCAGATTT+TGG + Chr1:3040014-3040033 None:intergenic 25.0%
!! TTTCTTTTATTCAAGCTTAG+GGG + Chr1:3038389-3038408 None:intergenic 25.0%
AAGAGCAAAATCTTGTAAAC+TGG - Chr1:3038329-3038348 MsG0180000221.01.T01:CDS 30.0%
AATAAGCTTGAAACAGCTTA+TGG - Chr1:3038231-3038250 MsG0180000221.01.T01:CDS 30.0%
AATGAGAATTTGGTCAAAAC+AGG - Chr1:3038021-3038040 MsG0180000221.01.T01:intron 30.0%
AATTTGGTCAAAACAGGTAA+TGG - Chr1:3038027-3038046 MsG0180000221.01.T01:intron 30.0%
ACTATTAGAGCTCTCAAATT+TGG + Chr1:3039444-3039463 None:intergenic 30.0%
ACTCTTTGATATCTTCCTTT+AGG + Chr1:3038730-3038749 None:intergenic 30.0%
ATCCAAATTATGTTGTTCCT+CGG - Chr1:3037755-3037774 MsG0180000221.01.T01:intron 30.0%
CAAATTGTTTCAATTCACAC+AGG - Chr1:3039202-3039221 MsG0180000221.01.T01:intron 30.0%
CAATAAAAATCTTCTCACCT+TGG - Chr1:3038874-3038893 MsG0180000221.01.T01:intron 30.0%
CATCTTTCTCATCAATATCA+TGG + Chr1:3038829-3038848 None:intergenic 30.0%
CTATTTCATTGAAGTCTCTT+AGG - Chr1:3040021-3040040 MsG0180000221.01.T01:intron 30.0%
GAAACAATTTGAGCTTCAAA+TGG + Chr1:3039193-3039212 None:intergenic 30.0%
GTGTAAACTTGAATTTCCTA+AGG - Chr1:3039355-3039374 MsG0180000221.01.T01:intron 30.0%
GTTAAAAACAGGTTATCTCA+AGG - Chr1:3038641-3038660 MsG0180000221.01.T01:intron 30.0%
TAACAGTATACAATGGAAAC+AGG - Chr1:3038532-3038551 MsG0180000221.01.T01:intron 30.0%
TACTGTTAGAATAACAATCC+TGG + Chr1:3038520-3038539 None:intergenic 30.0%
TATTTCACAATCTCCAAATG+TGG - Chr1:3037682-3037701 MsG0180000221.01.T01:CDS 30.0%
TGAAAACTGAAACATTGTGA+AGG + Chr1:3039493-3039512 None:intergenic 30.0%
TTACATTGTAGGTGAAATGA+AGG - Chr1:3039322-3039341 MsG0180000221.01.T01:intron 30.0%
TTGAATATGAACATTTGGCT+TGG + Chr1:3040099-3040118 None:intergenic 30.0%
TTGTTCCAAATTGTGACATT+CGG + Chr1:3039772-3039791 None:intergenic 30.0%
! AATTGATGGAGGGTAATTTT+GGG - Chr1:3037131-3037150 MsG0180000221.01.T01:CDS 30.0%
! AGATCAACCTGAAATGTTTT+GGG - Chr1:3038613-3038632 MsG0180000221.01.T01:intron 30.0%
! ATTTTTCATGAAAACACCTG+CGG + Chr1:3038292-3038311 None:intergenic 30.0%
! CAAGGTGAGAAGATTTTTAT+TGG + Chr1:3038876-3038895 None:intergenic 30.0%
!! TTTGGGAAAGTGTTAAAAAC+AGG - Chr1:3038630-3038649 MsG0180000221.01.T01:intron 30.0%
!!! AACAGTGTTTTGATTTGGAA+TGG - Chr1:3040126-3040145 MsG0180000221.01.T01:CDS 30.0%
!!! ATCAGAACAGTGTTTTGATT+TGG - Chr1:3040121-3040140 MsG0180000221.01.T01:CDS 30.0%
!!! TTAACAGTTGCTACTTTTGT+AGG - Chr1:3037629-3037648 MsG0180000221.01.T01:CDS 30.0%
AAAATTCATCACACACGTGA+AGG + Chr1:3039304-3039323 None:intergenic 35.0%
AAAGTTTCCATCCCTCAATT+TGG - Chr1:3039548-3039567 MsG0180000221.01.T01:intron 35.0%
AAATTCATCACACACGTGAA+GGG + Chr1:3039303-3039322 None:intergenic 35.0%
AAGATTTGGGGACACATAAT+AGG - Chr1:3037718-3037737 MsG0180000221.01.T01:intron 35.0%
ACACTTTCCCAAAACATTTC+AGG + Chr1:3038623-3038642 None:intergenic 35.0%
AGATGCTCTCATCAATATGT+GGG - Chr1:3038796-3038815 MsG0180000221.01.T01:intron 35.0%
AGCTAATATATGAGCTTGCA+TGG + Chr1:3036967-3036986 None:intergenic 35.0%
ATAACATCTAGAGGGTAAAG+AGG - Chr1:3039624-3039643 MsG0180000221.01.T01:intron 35.0%
ATAACTTGATCTATCAGTGC+TGG - Chr1:3039001-3039020 MsG0180000221.01.T01:intron 35.0%
ATGCAAATAGAGAACACACA+AGG + Chr1:3038473-3038492 None:intergenic 35.0%
CAAAATCTTGTAAACTGGAC+CGG - Chr1:3038334-3038353 MsG0180000221.01.T01:CDS 35.0%
CAGTGAATATTGTTGGGAAT+TGG + Chr1:3037910-3037929 None:intergenic 35.0%
CATGAGATTCTCCAATACTT+TGG + Chr1:3038667-3038686 None:intergenic 35.0%
CTTGGAAAAGATTGACATGA+GGG - Chr1:3039965-3039984 MsG0180000221.01.T01:intron 35.0%
GAACAACTTCCTGTTGAATT+AGG - Chr1:3039786-3039805 MsG0180000221.01.T01:intron 35.0%
GAAGAAGAAAGTTAAGGAGA+TGG - Chr1:3037184-3037203 MsG0180000221.01.T01:CDS 35.0%
GATGACTCAAATTAACTGCA+AGG + Chr1:3039084-3039103 None:intergenic 35.0%
GATTGGTAAACTAGTTAGCT+TGG - Chr1:3039947-3039966 MsG0180000221.01.T01:intron 35.0%
GCATTCTCATGGAAAACTTA+GGG - Chr1:3039573-3039592 MsG0180000221.01.T01:intron 35.0%
GCTAATATATGAGCTTGCAT+GGG + Chr1:3036966-3036985 None:intergenic 35.0%
TAATTACTCAATCAATGCGC+TGG + Chr1:3038061-3038080 None:intergenic 35.0%
TACTTGAAGAGAGGATTTGT+GGG - Chr1:3037207-3037226 MsG0180000221.01.T01:CDS 35.0%
TAGATGCTCTCATCAATATG+TGG - Chr1:3038795-3038814 MsG0180000221.01.T01:intron 35.0%
TATGTGGAAAGATGTTGAGA+AGG - Chr1:3040073-3040092 MsG0180000221.01.T01:intron 35.0%
TATTGTTGGGAATTGGAATC+TGG + Chr1:3037903-3037922 None:intergenic 35.0%
TGAGTGTGAAAATCTTCCAT+TGG - Chr1:3038562-3038581 MsG0180000221.01.T01:intron 35.0%
TGTAGGTGAAATGAAGGAAA+GGG - Chr1:3039328-3039347 MsG0180000221.01.T01:intron 35.0%
TGTATCCTACAAATGCTTGA+GGG + Chr1:3039721-3039740 None:intergenic 35.0%
TTATCTCAAGGCCAAAGTAT+TGG - Chr1:3038653-3038672 MsG0180000221.01.T01:intron 35.0%
TTCACTTCTGCATTCAATTG+CGG + Chr1:3037787-3037806 None:intergenic 35.0%
TTCCAACATGCTTACTGAAA+AGG + Chr1:3037327-3037346 None:intergenic 35.0%
TTGGCATTATCTATGAAAGG+AGG + Chr1:3039425-3039444 None:intergenic 35.0%
TTGTAGGTGAAATGAAGGAA+AGG - Chr1:3039327-3039346 MsG0180000221.01.T01:intron 35.0%
TTGTATCCTACAAATGCTTG+AGG + Chr1:3039722-3039741 None:intergenic 35.0%
TTTACAGCTTGCAAAGAAGA+TGG - Chr1:3036903-3036922 MsG0180000221.01.T01:CDS 35.0%
TTTGGGGAAGAAGAAAGTTA+AGG - Chr1:3037178-3037197 MsG0180000221.01.T01:CDS 35.0%
! AACATCATCTAGAACAACCA+AGG + Chr1:3037817-3037836 None:intergenic 35.0%
! ACTAGTTTACCAATCTCTTC+AGG + Chr1:3039942-3039961 None:intergenic 35.0%
! GAATTGATGGAGGGTAATTT+TGG - Chr1:3037130-3037149 MsG0180000221.01.T01:CDS 35.0%
! GAGATCAACCTGAAATGTTT+TGG - Chr1:3038612-3038631 MsG0180000221.01.T01:intron 35.0%
! GTATTTGGTGAGATGAAGAT+TGG - Chr1:3037051-3037070 MsG0180000221.01.T01:CDS 35.0%
! TAAGATCTCTAGAGATACTC+CGG - Chr1:3039814-3039833 MsG0180000221.01.T01:intron 35.0%
! TAGGCTTGTAATTTGTGATG+AGG - Chr1:3040040-3040059 MsG0180000221.01.T01:intron 35.0%
! TCATGTTTTCCTGAAGAGAT+TGG - Chr1:3039930-3039949 MsG0180000221.01.T01:intron 35.0%
! TTCCTTTTCAGTAAGCATGT+TGG - Chr1:3037322-3037341 MsG0180000221.01.T01:intron 35.0%
! TTGGTTGTTCTAGATGATGT+TGG - Chr1:3037816-3037835 MsG0180000221.01.T01:intron 35.0%
! TTTTCATGAAAAATCCCCCA+CGG - Chr1:3038298-3038317 MsG0180000221.01.T01:CDS 35.0%
!! AGTTGAGTGTTGGTTTGATT+TGG - Chr1:3037160-3037179 MsG0180000221.01.T01:CDS 35.0%
!! ATATTGATGAGAGCATCTAG+AGG + Chr1:3038794-3038813 None:intergenic 35.0%
!! ATTTGTGATGAGGAAGTGTT+TGG - Chr1:3040050-3040069 MsG0180000221.01.T01:intron 35.0%
!! GTGAGACTCAAGTTTAGTTT+TGG + Chr1:3039750-3039769 None:intergenic 35.0%
!! GTTGAGTGTTGGTTTGATTT+GGG - Chr1:3037161-3037180 MsG0180000221.01.T01:CDS 35.0%
!! TAGGAAGTTGGAGAAGTATT+TGG - Chr1:3037036-3037055 MsG0180000221.01.T01:CDS 35.0%
!! TAGTTAGTTTGCAATTGCTG+AGG - Chr1:3038950-3038969 MsG0180000221.01.T01:intron 35.0%
!! TTGAGTGTTGGTTTGATTTG+GGG - Chr1:3037162-3037181 MsG0180000221.01.T01:CDS 35.0%
!!! AGAGGTATTTTTTTGTCCTC+AGG + Chr1:3038776-3038795 None:intergenic 35.0%
!!! GAGGTATTTTTTTGTCCTCA+GGG + Chr1:3038775-3038794 None:intergenic 35.0%
!!! GGAGGAAGTGTTTTCAATAA+CGG + Chr1:3039852-3039871 None:intergenic 35.0%
ACAACAACAAACTTGCAACC+TGG + Chr1:3037878-3037897 None:intergenic 40.0%
ACAAGGTATGAGATGGTGAA+GGG - Chr1:3036999-3037018 MsG0180000221.01.T01:CDS 40.0%
AGCTTGAAAAGAACGTGTCA+AGG - Chr1:3036929-3036948 MsG0180000221.01.T01:CDS 40.0%
AGGAAGATGAGAATTGATGG+AGG - Chr1:3037120-3037139 MsG0180000221.01.T01:CDS 40.0%
ATGAGAATGCCTCCAAATTG+AGG + Chr1:3039563-3039582 None:intergenic 40.0%
ATGATCAGAACTTCAGCCTT+AGG + Chr1:3039374-3039393 None:intergenic 40.0%
CAAATTATGTTGTTCCTCGG+TGG - Chr1:3037758-3037777 MsG0180000221.01.T01:intron 40.0%
CACCGAGGAACAACATAATT+TGG + Chr1:3037760-3037779 None:intergenic 40.0%
GAACACACAAGGTTTGATCA+CGG + Chr1:3038462-3038481 None:intergenic 40.0%
GCTTGGAAAAGATTGACATG+AGG - Chr1:3039964-3039983 MsG0180000221.01.T01:intron 40.0%
GGAAGATGAGAATTGATGGA+GGG - Chr1:3037121-3037140 MsG0180000221.01.T01:CDS 40.0%
GGCATTCTCATGGAAAACTT+AGG - Chr1:3039572-3039591 MsG0180000221.01.T01:intron 40.0%
GTACTTGAAGAGAGGATTTG+TGG - Chr1:3037206-3037225 MsG0180000221.01.T01:CDS 40.0%
GTGAAATGAAGGAAAGGGAT+TGG - Chr1:3039333-3039352 MsG0180000221.01.T01:intron 40.0%
GTTATGCCAAAACGAGAGAA+AGG - Chr1:3039133-3039152 MsG0180000221.01.T01:intron 40.0%
GTTGTTGGTATTCATGGGAT+TGG - Chr1:3037229-3037248 MsG0180000221.01.T01:intron 40.0%
TCTCACCGAATGTCACAATT+TGG - Chr1:3039764-3039783 MsG0180000221.01.T01:intron 40.0%
TGAGAATGCCTCCAAATTGA+GGG + Chr1:3039562-3039581 None:intergenic 40.0%
TGAGGGAATGCTGTATGATT+AGG - Chr1:3039982-3040001 MsG0180000221.01.T01:intron 40.0%
TGGAGGAAGATGAGAATTGA+TGG - Chr1:3037117-3037136 MsG0180000221.01.T01:CDS 40.0%
TTGTTCTGTCACCATGCTTT+GGG - Chr1:3037974-3037993 MsG0180000221.01.T01:CDS 40.0%
TTTGTTCTGTCACCATGCTT+TGG - Chr1:3037973-3037992 MsG0180000221.01.T01:CDS 40.0%
! ATTGATTTCTGCCCAAAGCA+TGG + Chr1:3037988-3038007 None:intergenic 40.0%
! CATTCTACAGTCTGAGACAT+AGG - Chr1:3038420-3038439 MsG0180000221.01.T01:CDS 40.0%
! CCAATCACTTTAAGAGCCAA+TGG + Chr1:3038581-3038600 None:intergenic 40.0%
! GCTGTTTCATAATGCTATCG+TGG - Chr1:3038196-3038215 MsG0180000221.01.T01:CDS 40.0%
! GTCTCACAAGTGTGTTTTGT+TGG - Chr1:3037589-3037608 MsG0180000221.01.T01:CDS 40.0%
! TGTGGGTTGTTGGTATTCAT+GGG - Chr1:3037224-3037243 MsG0180000221.01.T01:intron 40.0%
! TGTGTCTCAACCACTCTTTT+GGG + Chr1:3039162-3039181 None:intergenic 40.0%
! TTGTGGGTTGTTGGTATTCA+TGG - Chr1:3037223-3037242 MsG0180000221.01.T01:intron 40.0%
! TTGTGTCTCAACCACTCTTT+TGG + Chr1:3039163-3039182 None:intergenic 40.0%
!! ATGAGGAAGTGTTTGGTATG+TGG - Chr1:3040057-3040076 MsG0180000221.01.T01:intron 40.0%
!! CCATTGGCTCTTAAAGTGAT+TGG - Chr1:3038578-3038597 MsG0180000221.01.T01:intron 40.0%
!! TCAATTTGGAGGCATTCTCA+TGG - Chr1:3039562-3039581 MsG0180000221.01.T01:intron 40.0%
!! TGGGAATTTGAGTTGAGTGT+TGG - Chr1:3037150-3037169 MsG0180000221.01.T01:CDS 40.0%
!!! TGGAAACTTTTTCAAGCCAC+AGG + Chr1:3039538-3039557 None:intergenic 40.0%
AATGCCTCCAAATTGAGGGA+TGG + Chr1:3039558-3039577 None:intergenic 45.0%
AGAAAGGACTCCCAAAAGAG+TGG - Chr1:3039149-3039168 MsG0180000221.01.T01:intron 45.0%
AGAGTGATCTCAGAGAGGTT+AGG + Chr1:3039670-3039689 None:intergenic 45.0%
AGCAGCTCAGGACAAGATTT+GGG - Chr1:3037705-3037724 MsG0180000221.01.T01:intron 45.0%
AGGAGATGGTACTTGAAGAG+AGG - Chr1:3037198-3037217 MsG0180000221.01.T01:CDS 45.0%
CAAGGTTTGATCACGGAGTT+TGG + Chr1:3038455-3038474 None:intergenic 45.0%
CACAAGGTATGAGATGGTGA+AGG - Chr1:3036998-3037017 MsG0180000221.01.T01:CDS 45.0%
CAGAAGTGAAGCTAGAACCT+TGG - Chr1:3037797-3037816 MsG0180000221.01.T01:intron 45.0%
CAGCTTGTGTGTAGAATACC+AGG - Chr1:3037857-3037876 MsG0180000221.01.T01:CDS 45.0%
CTTTGCCCTCAAGCATTTGT+AGG - Chr1:3039713-3039732 MsG0180000221.01.T01:intron 45.0%
GATCAAGAGTGATCTCAGAG+AGG + Chr1:3039675-3039694 None:intergenic 45.0%
GGTGAGATGAAGATTGGTGT+GGG - Chr1:3037057-3037076 MsG0180000221.01.T01:CDS 45.0%
GGTTGAACTGTTGAGTGAAG+AGG - Chr1:3037940-3037959 MsG0180000221.01.T01:CDS 45.0%
GTTGGTATTCATGGGATTGG+TGG - Chr1:3037232-3037251 MsG0180000221.01.T01:intron 45.0%
GTTTCCATCCCTCAATTTGG+AGG - Chr1:3039551-3039570 MsG0180000221.01.T01:intron 45.0%
TCACCATCTCATACCTTGTG+TGG + Chr1:3036998-3037017 None:intergenic 45.0%
TCACCTTGGTGAAAGAAGCA+CGG - Chr1:3038888-3038907 MsG0180000221.01.T01:intron 45.0%
TGGTGAGATGAAGATTGGTG+TGG - Chr1:3037056-3037075 MsG0180000221.01.T01:CDS 45.0%
TTCATCACACACAAGCACAC+AGG + Chr1:3039250-3039269 None:intergenic 45.0%
TTGCTCTTGAACAGTACCGT+GGG + Chr1:3038317-3038336 None:intergenic 45.0%
TTTGCTCTTGAACAGTACCG+TGG + Chr1:3038318-3038337 None:intergenic 45.0%
! ACTTGATCTATCAGTGCTGG+TGG - Chr1:3039004-3039023 MsG0180000221.01.T01:intron 45.0%
! AGAGAGGATTTGTGGGTTGT+TGG - Chr1:3037214-3037233 MsG0180000221.01.T01:CDS 45.0%
! ATTCATGGGATTGGTGGATC+TGG - Chr1:3037238-3037257 MsG0180000221.01.T01:intron 45.0%
! GAACGTGTCAAGGTTTTGCA+TGG - Chr1:3036939-3036958 MsG0180000221.01.T01:CDS 45.0%
! TTCATGGGATTGGTGGATCT+GGG - Chr1:3037239-3037258 MsG0180000221.01.T01:intron 45.0%
!! ATAACGGACACGCGTATAAC+CGG + Chr1:3039836-3039855 None:intergenic 45.0%
AGACTTGTGTGCATTCCCTG+AGG - Chr1:3038757-3038776 MsG0180000221.01.T01:intron 50.0%
AGATGGTGAAGGGTTCGATC+GGG - Chr1:3037009-3037028 MsG0180000221.01.T01:CDS 50.0%
ATGAAGATTGGTGTGGGAGG+TGG - Chr1:3037063-3037082 MsG0180000221.01.T01:CDS 50.0%
CGAGTCATGTCACAAACGCT+AGG + Chr1:3039873-3039892 None:intergenic 50.0%
CTTAGCGCGCCTAATTCAAC+AGG + Chr1:3039798-3039817 None:intergenic 50.0%
GAAAACACCTGCGGCTCTTT+GGG + Chr1:3038283-3038302 None:intergenic 50.0%
GAGATGAAGATTGGTGTGGG+AGG - Chr1:3037060-3037079 MsG0180000221.01.T01:CDS 50.0%
GAGATGGTGAAGGGTTCGAT+CGG - Chr1:3037008-3037027 MsG0180000221.01.T01:CDS 50.0%
GAGCAGCTCAGGACAAGATT+TGG - Chr1:3037704-3037723 MsG0180000221.01.T01:intron 50.0%
GCACCACACAAGGTATGAGA+TGG - Chr1:3036992-3037011 MsG0180000221.01.T01:CDS 50.0%
GCAGCTCAGGACAAGATTTG+GGG - Chr1:3037706-3037725 MsG0180000221.01.T01:intron 50.0%
GTACCGTGCTTCTTTCACCA+AGG + Chr1:3038894-3038913 None:intergenic 50.0%
GTCATGTCACAAACGCTAGG+AGG + Chr1:3039870-3039889 None:intergenic 50.0%
TGAAAACACCTGCGGCTCTT+TGG + Chr1:3038284-3038303 None:intergenic 50.0%
TGAAGATTGGTGTGGGAGGT+GGG - Chr1:3037064-3037083 MsG0180000221.01.T01:CDS 50.0%
TGCTCTTGAACAGTACCGTG+GGG + Chr1:3038316-3038335 None:intergenic 50.0%
TGGCAAACTTGAGAAGCCTG+TGG - Chr1:3039519-3039538 MsG0180000221.01.T01:intron 50.0%
TTCAATTGCGGTATCCACCG+AGG + Chr1:3037775-3037794 None:intergenic 50.0%
! AGCTGTGAGGTCTTGTATGG+AGG - Chr1:3037100-3037119 MsG0180000221.01.T01:CDS 50.0%
! ATCACACACGTGAAGGGCAT+CGG + Chr1:3039297-3039316 None:intergenic 50.0%
! GGAAGCTGTGAGGTCTTGTA+TGG - Chr1:3037097-3037116 MsG0180000221.01.T01:CDS 50.0%
! GGGAGTCCTTTCTCTCGTTT+TGG + Chr1:3039142-3039161 None:intergenic 50.0%
! GTCTGAGACATAGGTGCACT+TGG - Chr1:3038429-3038448 MsG0180000221.01.T01:CDS 50.0%
!! AAGGGTTCGATCGGGTTGAT+AGG - Chr1:3037017-3037036 MsG0180000221.01.T01:CDS 50.0%
!! CGATCGGGTTGATAGGAAGT+TGG - Chr1:3037024-3037043 MsG0180000221.01.T01:CDS 50.0%
AAGATTGGTGTGGGAGGTGG+GGG - Chr1:3037066-3037085 MsG0180000221.01.T01:CDS 55.0%
AAGCTATCCCAAAGAGCCGC+AGG - Chr1:3038273-3038292 MsG0180000221.01.T01:CDS 55.0%
CTCCAAATGTGGAGCAGCTC+AGG - Chr1:3037693-3037712 MsG0180000221.01.T01:CDS 55.0%
GAAGATTGGTGTGGGAGGTG+GGG - Chr1:3037065-3037084 MsG0180000221.01.T01:CDS 55.0%
GCTCTTGAACAGTACCGTGG+GGG + Chr1:3038315-3038334 None:intergenic 55.0%
GTCCTGAGCTGCTCCACATT+TGG + Chr1:3037698-3037717 None:intergenic 55.0%
TAGCTGACGTGCACCACACA+AGG - Chr1:3036982-3037001 MsG0180000221.01.T01:CDS 55.0%
ATTGGTGTGGGAGGTGGGGG+TGG - Chr1:3037069-3037088 MsG0180000221.01.T01:CDS 65.0%
GGTGGGTGCAGGAAGCTGTG+AGG - Chr1:3037087-3037106 MsG0180000221.01.T01:CDS 65.0%
TTGGTGTGGGAGGTGGGGGT+GGG - Chr1:3037070-3037089 MsG0180000221.01.T01:CDS 65.0%
TGGGAGGTGGGGGTGGGTGC+AGG - Chr1:3037076-3037095 MsG0180000221.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 3036886 3040160 3036886 ID=MsG0180000221.01;Name=MsG0180000221.01
Chr1 mRNA 3036886 3040160 3036886 ID=MsG0180000221.01.T01;Parent=MsG0180000221.01;Name=MsG0180000221.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0.5|0.2|1|0.75|0.8|5|0|428
Chr1 exon 3040104 3040160 3040104 ID=MsG0180000221.01.T01:exon:6320;Parent=MsG0180000221.01.T01
Chr1 exon 3038137 3038525 3038137 ID=MsG0180000221.01.T01:exon:6321;Parent=MsG0180000221.01.T01
Chr1 exon 3037823 3038028 3037823 ID=MsG0180000221.01.T01:exon:6322;Parent=MsG0180000221.01.T01
Chr1 exon 3037432 3037713 3037432 ID=MsG0180000221.01.T01:exon:6323;Parent=MsG0180000221.01.T01
Chr1 exon 3036886 3037238 3036886 ID=MsG0180000221.01.T01:exon:6324;Parent=MsG0180000221.01.T01
Chr1 CDS 3040104 3040160 3040104 ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Chr1 CDS 3038137 3038525 3038137 ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Chr1 CDS 3037823 3038028 3037823 ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Chr1 CDS 3037432 3037713 3037432 ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Chr1 CDS 3036886 3037238 3036886 ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Gene Sequence

>MsG0180000221.01.T01

ATGGAACGTGTACAAGATTTACAGCTTGCAAAGAAGATGGAGAAGCTTGAAAAGAACGATTGTTATTCTAACAGTATACAATGGAAACAGGTTGTGAGTGAGTGTGAAAATCTTCCATTGGCTCTTAAAGTGATTGGAGCTTCATTGAGAGATCAACCTGAAATGTTTTGGGAAAGTGTTAAAAACAGGTTATCTCAAGGCCAAAGTATTGGAGAATCTCATGAAATCAACCTAATTGAAAGAATGGCAATAAGTATCAACTACCTAAAGGAAGATATCAAAGAGTGCTTCTTAGACTTGTGTGCATTCCCTGAGGACAAAAAAATACCTCTAGATGCTCTCATCAATATGTGGGTTGAAATCCATGATATTGATGAGAAAGATGCTTTTGCTATTGTTGTTGAGCTCTCCAATAAAAATCTTCTCACCTTGGTGAAAGAAGCACGTGCTGGTGGCATGTACAGCAGTTGCTTTGAGATCTCAGTTACTCAACATGATGTGCTTAGAGACCTTGCAGTTAATTTGAGTCATCGCGAAAGTATTAATGAACGACGACGATTAGTTATGCCAAAACGAGAGAAAGGACTCCCAAAAGAGTGGTTGAGACACAAACACAAACCATTTGAAGCTCAAATTGTTTCAATTCACACAGGTGAAATGAAGGAAAGGGATTGGTGTAAACTTGAATTTCCTAAGGCTGAAGTTCTGATCATAAATTTCACATCTAAGGATTACTTTTTACCTCCTTTCATAGATAATGCCAAATTTGAGAGCTCTAATAGTAATAACTATAGTGCATCATATGCATGCCTTCACAATGTTTCAGTTTTCAACAATTTGGCAAACTTGAGAAGCCTGTGGCTTGAAAAAGTTTCCATCCCTCAATTTGGAGGCATTCTCATGGAAAACTTAGGGAAGTTATTTATAGTTTCATGCGCGCTAAGATCTCTAGAGATACTCCGGTTATACGCGTGTCCGTTATTGAAAACACTTCCTCCTAGCGTTTGTGACATGACTCGATTGAAGTATATCGATGTTTCACAATGTGTTAACTTTTCATGTTTTCCTGAAGAGATTGGTAAACTAGTTAGCTTGGAAAAGATTGACATGAGGGAATGCTGTATGATTAGGAAAGTACCAAAATCTGCTATTTCATTGAAGTCTCTTAGGCTTGTAATTTGTGATGAGGAAGTGTTTGGTATGTGGAAAGATGTTGAGAAGGCCAAGCCAAATGTTCATATTCAAGTATCAGAACAGTGTTTTGATTTGGAATGGCTTAGTGAGTAA
Protein sequence

>MsG0180000221.01.T01

MERVQDLQLAKKMEKLEKNDCYSNSIQWKQVVSECENLPLALKVIGASLRDQPEMFWESVKNRLSQGQSIGESHEINLIERMAISINYLKEDIKECFLDLCAFPEDKKIPLDALINMWVEIHDIDEKDAFAIVVELSNKNLLTLVKEARAGGMYSSCFEISVTQHDVLRDLAVNLSHRESINERRRLVMPKREKGLPKEWLRHKHKPFEAQIVSIHTGEMKERDWCKLEFPKAEVLIINFTSKDYFLPPFIDNAKFESSNSNNYSASYACLHNVSVFNNLANLRSLWLEKVSIPQFGGILMENLGKLFIVSCALRSLEILRLYACPLLKTLPPSVCDMTRLKYIDVSQCVNFSCFPEEIGKLVSLEKIDMRECCMIRKVPKSAISLKSLRLVICDEEVFGMWKDVEKAKPNVHIQVSEQCFDLEWLSE*