AlfalfaGEDB | MsG0180000221.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000221.01

KEGG: -
GO: GO:0043531
PFAM: PF00931

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000221.01.T01	XP_003589251.2	80.819	464	25	3	29	428	361	824	0	729

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000221.01.T01	Q9SZA7	47.854	466	176	5	29	428	352	816	8.36E-140	422

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000097.01	MsG0180000221.01	-0.848790	4.897599e-60	9.091368e-57
MsG0180000151.01	MsG0180000221.01	0.822148	2.765463e-53	2.295571e-50
MsG0180000221.01	MsG0180001119.01	0.804807	1.809379e-49	9.418989e-47
MsG0180000221.01	MsG0180004112.01	0.803597	3.231926e-49	1.630803e-46
MsG0180000221.01	MsG0180005457.01	0.813278	2.775431e-51	1.804571e-48
MsG0180000221.01	MsG0180005639.01	0.836924	7.035037e-57	8.996009e-54
MsG0180000221.01	MsG0180005729.01	0.820039	8.467524e-53	6.626639e-50
MsG0180000221.01	MsG0180005731.01	0.810648	1.038214e-50	6.293451e-48
MsG0180000221.01	MsG0180006054.01	0.805445	1.330127e-49	7.038372e-47
MsG0180000221.01	MsG0280007864.01	-0.806447	8.191328e-50	4.447026e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000221.01.T01	MTR_1g021100	80.819	464	25	3	29	428	361	824	0.0	729
MsG0180000221.01.T01	MTR_1g021110	63.305	466	105	4	29	428	358	823	0.0	559
MsG0180000221.01.T01	MTR_0391s0020	28.484	488	254	12	29	426	334	816	2.50e-56	199
MsG0180000221.01.T01	MTR_8g079360	28.279	488	253	12	29	426	324	804	3.60e-56	198
MsG0180000221.01.T01	MTR_8g479430	31.183	465	251	9	29	426	349	811	7.10e-56	197
MsG0180000221.01.T01	MTR_5g018060	30.754	504	248	14	17	426	328	824	4.66e-55	196
MsG0180000221.01.T01	MTR_5g018070	33.168	404	221	10	30	390	263	660	5.61e-55	194
MsG0180000221.01.T01	MTR_5g018120	34.439	392	218	12	30	389	329	713	7.99e-55	195
MsG0180000221.01.T01	MTR_8g079400	27.926	487	256	12	29	426	300	780	9.12e-53	189
MsG0180000221.01.T01	MTR_8g079400	27.926	487	256	12	29	426	310	790	1.38e-52	188

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000221.01.T01	AT4G33300	47.854	466	176	5	29	428	352	816	8.52e-141	422
MsG0180000221.01.T01	AT4G33300	47.854	466	176	5	29	428	352	816	8.52e-141	422
MsG0180000221.01.T01	AT5G04720	42.489	466	201	5	29	428	347	811	2.68e-126	385
MsG0180000221.01.T01	AT1G33560	42.979	470	202	5	24	428	319	787	8.51e-123	375
MsG0180000221.01.T01	AT5G47280	41.416	466	206	5	29	428	159	623	2.01e-120	364
MsG0180000221.01.T01	AT5G66910	28.750	480	259	12	20	426	343	812	7.56e-49	178
MsG0180000221.01.T01	AT5G66900	28.571	469	254	13	20	418	341	798	4.95e-46	170
MsG0180000221.01.T01	AT5G66900	28.571	469	254	13	20	418	389	846	1.03e-45	169
MsG0180000221.01.T01	AT5G66890	28.607	402	206	12	85	414	8	400	1.24e-33	130
MsG0180000221.01.T01	AT5G66900	30.693	202	128	5	20	218	389	581	6.28e-20	92.8

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Find 67 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTTCCATCCCTCAATT+TGG	0.261702	1:-3037476	MsG0180000221.01.T01:CDS
AATAAGTATCAACTACCTAA+AGG	0.566013	1:-3038312	MsG0180000221.01.T01:CDS
AATCAACCTAATTGAAAGAA+TGG	0.452666	1:-3038336	MsG0180000221.01.T01:CDS
AATGCCTCCAAATTGAGGGA+TGG	0.626787	1:+3037469	None:intergenic
AATTTGGCATTATCTATGAA+AGG	0.596417	1:+3037599	None:intergenic
ACACTTTCCCAAAACATTTC+AGG	0.255125	1:+3038404	None:intergenic
ACTAGTTTACCAATCTCTTC+AGG	0.357302	1:+3037085	None:intergenic
ACTATTAGAGCTCTCAAATT+TGG	0.198230	1:+3037583	None:intergenic
ACTCTTTGATATCTTCCTTT+AGG	0.293617	1:+3038297	None:intergenic
ACTTGATCTATCAGTGCTGG+TGG	0.615020	1:-3038020	MsG0180000221.01.T01:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAGCAAAATCTTGTAAAC+TGG	-	Chr1:3038329-3038348	MsG0180000221.01.T01:CDS	30.0%
AATAAGCTTGAAACAGCTTA+TGG	-	Chr1:3038231-3038250	MsG0180000221.01.T01:CDS	30.0%
AATGAGAATTTGGTCAAAAC+AGG	-	Chr1:3038021-3038040	MsG0180000221.01.T01:intron	30.0%
AATTTGGTCAAAACAGGTAA+TGG	-	Chr1:3038027-3038046	MsG0180000221.01.T01:intron	30.0%
ACTATTAGAGCTCTCAAATT+TGG	+	Chr1:3039444-3039463	None:intergenic	30.0%
ACTCTTTGATATCTTCCTTT+AGG	+	Chr1:3038730-3038749	None:intergenic	30.0%
ATCCAAATTATGTTGTTCCT+CGG	-	Chr1:3037755-3037774	MsG0180000221.01.T01:intron	30.0%
CAAATTGTTTCAATTCACAC+AGG	-	Chr1:3039202-3039221	MsG0180000221.01.T01:intron	30.0%
CAATAAAAATCTTCTCACCT+TGG	-	Chr1:3038874-3038893	MsG0180000221.01.T01:intron	30.0%
CATCTTTCTCATCAATATCA+TGG	+	Chr1:3038829-3038848	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	3036886	3040160	3036886	ID=MsG0180000221.01;Name=MsG0180000221.01
Chr1	mRNA	3036886	3040160	3036886	ID=MsG0180000221.01.T01;Parent=MsG0180000221.01;Name=MsG0180000221.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|0.5\|0.2\|1\|0.75\|0.8\|5\|0\|428
Chr1	exon	3040104	3040160	3040104	ID=MsG0180000221.01.T01:exon:6320;Parent=MsG0180000221.01.T01
Chr1	exon	3038137	3038525	3038137	ID=MsG0180000221.01.T01:exon:6321;Parent=MsG0180000221.01.T01
Chr1	exon	3037823	3038028	3037823	ID=MsG0180000221.01.T01:exon:6322;Parent=MsG0180000221.01.T01
Chr1	exon	3037432	3037713	3037432	ID=MsG0180000221.01.T01:exon:6323;Parent=MsG0180000221.01.T01
Chr1	exon	3036886	3037238	3036886	ID=MsG0180000221.01.T01:exon:6324;Parent=MsG0180000221.01.T01
Chr1	CDS	3040104	3040160	3040104	ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Chr1	CDS	3038137	3038525	3038137	ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Chr1	CDS	3037823	3038028	3037823	ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Chr1	CDS	3037432	3037713	3037432	ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01
Chr1	CDS	3036886	3037238	3036886	ID=MsG0180000221.01.T01:cds;Parent=MsG0180000221.01.T01

Gene Sequence

>MsG0180000221.01.T01

ATGGAACGTGTACAAGATTTACAGCTTGCAAAGAAGATGGAGAAGCTTGAAAAGAACGATTGTTATTCTAACAGTATACAATGGAAACAGGTTGTGAGTGAGTGTGAAAATCTTCCATTGGCTCTTAAAGTGATTGGAGCTTCATTGAGAGATCAACCTGAAATGTTTTGGGAAAGTGTTAAAAACAGGTTATCTCAAGGCCAAAGTATTGGAGAATCTCATGAAATCAACCTAATTGAAAGAATGGCAATAAGTATCAACTACCTAAAGGAAGATATCAAAGAGTGCTTCTTAGACTTGTGTGCATTCCCTGAGGACAAAAAAATACCTCTAGATGCTCTCATCAATATGTGGGTTGAAATCCATGATATTGATGAGAAAGATGCTTTTGCTATTGTTGTTGAGCTCTCCAATAAAAATCTTCTCACCTTGGTGAAAGAAGCACGTGCTGGTGGCATGTACAGCAGTTGCTTTGAGATCTCAGTTACTCAACATGATGTGCTTAGAGACCTTGCAGTTAATTTGAGTCATCGCGAAAGTATTAATGAACGACGACGATTAGTTATGCCAAAACGAGAGAAAGGACTCCCAAAAGAGTGGTTGAGACACAAACACAAACCATTTGAAGCTCAAATTGTTTCAATTCACACAGGTGAAATGAAGGAAAGGGATTGGTGTAAACTTGAATTTCCTAAGGCTGAAGTTCTGATCATAAATTTCACATCTAAGGATTACTTTTTACCTCCTTTCATAGATAATGCCAAATTTGAGAGCTCTAATAGTAATAACTATAGTGCATCATATGCATGCCTTCACAATGTTTCAGTTTTCAACAATTTGGCAAACTTGAGAAGCCTGTGGCTTGAAAAAGTTTCCATCCCTCAATTTGGAGGCATTCTCATGGAAAACTTAGGGAAGTTATTTATAGTTTCATGCGCGCTAAGATCTCTAGAGATACTCCGGTTATACGCGTGTCCGTTATTGAAAACACTTCCTCCTAGCGTTTGTGACATGACTCGATTGAAGTATATCGATGTTTCACAATGTGTTAACTTTTCATGTTTTCCTGAAGAGATTGGTAAACTAGTTAGCTTGGAAAAGATTGACATGAGGGAATGCTGTATGATTAGGAAAGTACCAAAATCTGCTATTTCATTGAAGTCTCTTAGGCTTGTAATTTGTGATGAGGAAGTGTTTGGTATGTGGAAAGATGTTGAGAAGGCCAAGCCAAATGTTCATATTCAAGTATCAGAACAGTGTTTTGATTTGGAATGGCTTAGTGAGTAA

Protein sequence

>MsG0180000221.01.T01

MERVQDLQLAKKMEKLEKNDCYSNSIQWKQVVSECENLPLALKVIGASLRDQPEMFWESVKNRLSQGQSIGESHEINLIERMAISINYLKEDIKECFLDLCAFPEDKKIPLDALINMWVEIHDIDEKDAFAIVVELSNKNLLTLVKEARAGGMYSSCFEISVTQHDVLRDLAVNLSHRESINERRRLVMPKREKGLPKEWLRHKHKPFEAQIVSIHTGEMKERDWCKLEFPKAEVLIINFTSKDYFLPPFIDNAKFESSNSNNYSASYACLHNVSVFNNLANLRSLWLEKVSIPQFGGILMENLGKLFIVSCALRSLEILRLYACPLLKTLPPSVCDMTRLKYIDVSQCVNFSCFPEEIGKLVSLEKIDMRECCMIRKVPKSAISLKSLRLVICDEEVFGMWKDVEKAKPNVHIQVSEQCFDLEWLSE*