AlfalfaGEDB | MsG0180005731.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005731.01

KEGG: K00913
GO: GO:0000287 GO:0005524 GO:0005622 GO:0032957 GO:0047325 GO:0052725 GO:0052726
PFAM: PF05770

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005731.01.T01	KEH43981.1	89.736	341	6	1	1	341	1	312	0	585

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005731.01.T01	Q9SUG3	75.794	252	59	1	90	341	101	350	3.48E-128	374

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005731.01.T01	A0A072VQP8	89.736	341	6	1	1	341	1	312	0.0	585

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048660.01	MsG0180005731.01	0.814187	1.750261e-51	1.166454e-48
MsG0180000160.01	MsG0180005731.01	0.824671	7.113753e-54	6.344306e-51
MsG0180000221.01	MsG0180005731.01	0.810648	1.038214e-50	6.293451e-48
MsG0180000241.01	MsG0180005731.01	0.812131	4.946010e-51	3.118843e-48
MsG0180000627.01	MsG0180005731.01	0.844071	9.487970e-59	1.514284e-55
MsG0180001088.01	MsG0180005731.01	0.815461	9.138176e-52	6.304485e-49
MsG0180005639.01	MsG0180005731.01	0.818759	1.657938e-52	1.252099e-49
MsG0180005729.01	MsG0180005731.01	0.993989	1.014233e-203	9.850050e-197
MsG0180005730.01	MsG0180005731.01	0.823486	1.349764e-53	1.163781e-50
MsG0180005731.01	MsG0180005732.01	0.831767	1.385106e-55	1.518041e-52

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005731.01.T01	MTR_1g105930	89.736	341	6	1	1	341	1	312	0.0	585
MsG0180005731.01.T01	MTR_1g105930	97.619	252	6	0	90	341	91	342	0.0	509
MsG0180005731.01.T01	MTR_5g005370	79.283	251	50	1	86	334	55	305	7.31e-133	381
MsG0180005731.01.T01	MTR_5g005370	79.268	246	51	0	89	334	36	281	3.07e-132	379
MsG0180005731.01.T01	MTR_5g005370	79.592	245	50	0	90	334	72	316	4.43e-132	379
MsG0180005731.01.T01	MTR_5g005370	79.268	246	51	0	89	334	43	288	7.48e-132	378
MsG0180005731.01.T01	MTR_5g005370	79.268	246	51	0	90	335	72	317	4.77e-131	379
MsG0180005731.01.T01	MTR_8g066720	42.294	279	138	8	76	339	54	324	8.51e-62	202
MsG0180005731.01.T01	MTR_4g105690	42.742	248	133	4	91	332	69	313	1.57e-61	199
MsG0180005731.01.T01	MTR_3g067725	28.151	238	156	8	91	318	236	468	3.88e-16	79.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005731.01.T01	AT4G08170	75.794	252	59	1	90	341	13	262	1.08e-129	371
MsG0180005731.01.T01	AT4G08170	75.794	252	59	1	90	341	101	350	3.55e-129	374
MsG0180005731.01.T01	AT4G08170	76.682	223	50	1	119	341	1	221	1.58e-113	329
MsG0180005731.01.T01	AT4G08170	76.682	223	50	1	119	341	1	221	1.58e-113	329
MsG0180005731.01.T01	AT4G08170	76.682	223	50	1	119	341	1	221	1.58e-113	329
MsG0180005731.01.T01	AT4G08170	76.682	223	50	1	119	341	1	221	1.58e-113	329
MsG0180005731.01.T01	AT4G08170	76.682	223	50	1	119	341	1	221	1.58e-113	329
MsG0180005731.01.T01	AT4G33770	65.462	249	84	1	90	338	48	294	4.70e-109	320
MsG0180005731.01.T01	AT4G33770	65.462	249	84	1	90	338	141	387	4.24e-108	321
MsG0180005731.01.T01	AT4G33770	66.364	220	72	1	119	338	1	218	1.15e-94	281

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Find 83 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGCTTCGTCATAGAT+TGG	0.446522	1:-95392580	MsG0180005731.01.T01:intron
AAAGTTTGCACTACTTTATT+TGG	0.121328	1:+95391705	None:intergenic
AAATCCTTCATTAATGAAAA+AGG	0.178226	1:+95394178	None:intergenic
AAATGTTGAATTGCATCAGG+AGG	0.678092	1:+95394121	None:intergenic
AAATTATGTCAGAAGTTGTG+TGG	0.741730	1:+95394874	None:intergenic
AACTCTTGTAGAACCAGTGG+AGG	0.639928	1:+95393001	None:intergenic
AAGCATATAACTAGCTACAA+AGG	0.610187	1:+95391730	None:intergenic
AATAGAATGTTCATGCTTAG+TGG	0.358768	1:-95391903	MsG0180005731.01.T01:three_prime_UTR
AATCGTTAGTATTATTAGTT+TGG	0.257470	1:-95394949	MsG0180005731.01.T01:CDS
AATGACATAAAACACATCCT+TGG	0.546362	1:+95392428	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTATGTCAGAAGTTGTG+TGG	+	Chr1:95391763-95391782	None:intergenic	30.0%
AAGCATATAACTAGCTACAA+AGG	+	Chr1:95394907-95394926	None:intergenic	30.0%
AATAACACACAGAATGATTG+CGG	+	Chr1:95393691-95393710	None:intergenic	30.0%
AATAGAATGTTCATGCTTAG+TGG	-	Chr1:95394731-95394750	MsG0180005731.01.T01:intron	30.0%
AATCAAAGATACACACCTAA+CGG	+	Chr1:95392966-95392985	None:intergenic	30.0%
AATCTTCTGATTATCCTGAT+TGG	-	Chr1:95393436-95393455	MsG0180005731.01.T01:intron	30.0%
AATGACATAAAACACATCCT+TGG	+	Chr1:95394209-95394228	None:intergenic	30.0%
AATTTCTTAAAGAACAAGGC+TGG	-	Chr1:95392048-95392067	MsG0180005731.01.T01:three_prime_UTR	30.0%
ACAGACAATAATTCGTTCTT+TGG	+	Chr1:95391925-95391944	None:intergenic	30.0%
ACTATCACAATAATATGGCA+TGG	-	Chr1:95392019-95392038	MsG0180005731.01.T01:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	95391671	95394985	95391671	ID=MsG0180005731.01;Name=MsG0180005731.01
Chr1	mRNA	95391671	95394985	95391671	ID=MsG0180005731.01.T01;Parent=MsG0180005731.01;Name=MsG0180005731.01.T01;_AED=0.44;_eAED=0.46;_QI=0\|0.85\|0.75\|1\|0.85\|0.75\|8\|554\|341
Chr1	exon	95391671	95392316	95391671	ID=MsG0180005731.01.T01:exon:10472;Parent=MsG0180005731.01.T01
Chr1	exon	95392409	95392493	95392409	ID=MsG0180005731.01.T01:exon:10471;Parent=MsG0180005731.01.T01
Chr1	exon	95392581	95392636	95392581	ID=MsG0180005731.01.T01:exon:10470;Parent=MsG0180005731.01.T01
Chr1	exon	95392718	95392894	95392718	ID=MsG0180005731.01.T01:exon:10469;Parent=MsG0180005731.01.T01
Chr1	exon	95392990	95393109	95392990	ID=MsG0180005731.01.T01:exon:10468;Parent=MsG0180005731.01.T01
Chr1	exon	95393687	95393785	95393687	ID=MsG0180005731.01.T01:exon:10467;Parent=MsG0180005731.01.T01
Chr1	exon	95394062	95394269	95394062	ID=MsG0180005731.01.T01:exon:10466;Parent=MsG0180005731.01.T01
Chr1	exon	95394797	95394985	95394797	ID=MsG0180005731.01.T01:exon:10465;Parent=MsG0180005731.01.T01
Chr1	CDS	95394797	95394985	95394797	ID=MsG0180005731.01.T01:cds;Parent=MsG0180005731.01.T01
Chr1	CDS	95394062	95394269	95394062	ID=MsG0180005731.01.T01:cds;Parent=MsG0180005731.01.T01
Chr1	CDS	95393687	95393785	95393687	ID=MsG0180005731.01.T01:cds;Parent=MsG0180005731.01.T01
Chr1	CDS	95392990	95393109	95392990	ID=MsG0180005731.01.T01:cds;Parent=MsG0180005731.01.T01
Chr1	CDS	95392718	95392894	95392718	ID=MsG0180005731.01.T01:cds;Parent=MsG0180005731.01.T01
Chr1	CDS	95392581	95392636	95392581	ID=MsG0180005731.01.T01:cds;Parent=MsG0180005731.01.T01
Chr1	CDS	95392409	95392493	95392409	ID=MsG0180005731.01.T01:cds;Parent=MsG0180005731.01.T01
Chr1	CDS	95392225	95392316	95392225	ID=MsG0180005731.01.T01:cds;Parent=MsG0180005731.01.T01
Chr1	three_prime_UTR	95391671	95392224	95391671	ID=MsG0180005731.01.T01:three_prime_utr;Parent=MsG0180005731.01.T01

Gene Sequence

>MsG0180005731.01.T01

ATGGTATTGTATGAAATCGTTAGTATTATTAGTTTGGGAGTTGAGTATGACATGGAGGTGATTGGCCGGACAACCACATCTTCTGGATACCACACAACTTCTGACATAATTTGCAGAGTTCTTGCTCTCCTCATCATGAATCATGATCAGATAGTTGAAATCAGTCCCCCAAACCAAGTATTCAAACAAGGATTGCTTCATGCTTTCCAAATTCTCCATGAAGTTTGTATTTTGATGGAGCTGATTGGACTTCTTTCCCCTTTTTCATTAATGAAGGATTTCAGGCAAAAACATCCCGATGTATTCGTTCTTGATCCTCCTGATGCAATTCAACATTTACACAACCGCCAATCCATGCTTCAAGATGTGGTGGATCTAAACTTATCTGAGTGCAATGGCAAGGTTGGCATCCCTCGTCAGCTAGTTATTACAAAAGACAAAGATCCATCTACTATTCCTTATGAAGTCACCAAAGCTGGGTTGAAGTTGCCGTTAGTTGCAAAACCTCTAGTTGTGGATGGCAGTGCAAAGTCGCATGAACTATTTCTTGCTTATGACGAGTTCTCTCTTTCAGAGCTTGAACCTCCACTGGTTCTACAAGAGTTTGTCAATCATGGTGGTCTTCTCTTTAAGATTTATATTGTTGGGGAATACATAAAGGTTGTGAGGCGTTTCTCTCTTCCTAATATCAGTAAACGCGAGCTATCAAAAGTCGCAGGTGTATACCGTTTTCCAAGAGTTTCATGTGCAGCAGCTTCTGCAGATGAAGCTGATCTTGATCCTAGTATTGCCGAACACCCACCAAGACCTTTGTTGGAGAGGCTTGCAAAAGAGCTTCGTCATAGATTGGGACTTCGCTTGTTCAACGTAGATATGATTCGTGAATATGGGACCAAGGATGTGTTTTATGTCATTGATATCAATTACTTTCCTGGGTATGGAAAAATGCCAGACTACGAGCACGTCTTTACAGATTTTCTACTTAACCTAAAGCAGAACAAGTGTAAGAAGAAACTTACTGCCTAA

Protein sequence

>MsG0180005731.01.T01

MVLYEIVSIISLGVEYDMEVIGRTTTSSGYHTTSDIICRVLALLIMNHDQIVEISPPNQVFKQGLLHAFQILHEVCILMELIGLLSPFSLMKDFRQKHPDVFVLDPPDAIQHLHNRQSMLQDVVDLNLSECNGKVGIPRQLVITKDKDPSTIPYEVTKAGLKLPLVAKPLVVDGSAKSHELFLAYDEFSLSELEPPLVLQEFVNHGGLLFKIYIVGEYIKVVRRFSLPNISKRELSKVAGVYRFPRVSCAAASADEADLDPSIAEHPPRPLLERLAKELRHRLGLRLFNVDMIREYGTKDVFYVIDINYFPGYGKMPDYEHVFTDFLLNLKQNKCKKKLTA*