AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041080.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041080.01.T01 MTR_7g103900 95.806 310 9 2 1 306 1 310 0.0 603
MsG0780041080.01.T01 MTR_7g103900 94.760 229 11 1 1 228 1 229 1.14e-157 439
MsG0780041080.01.T01 MTR_4g055230 52.090 311 137 10 1 305 1 305 1.32e-95 285
MsG0780041080.01.T01 MTR_2g084965 49.838 309 140 9 4 304 6 307 3.13e-83 253
MsG0780041080.01.T01 MTR_7g095100 35.357 280 154 9 1 273 1 260 6.53e-49 165
MsG0780041080.01.T01 MTR_7g114790 35.870 276 153 7 1 273 1 255 1.40e-48 164
MsG0780041080.01.T01 MTR_1g081260 32.919 322 179 9 1 305 1 302 1.11e-46 159
MsG0780041080.01.T01 MTR_3g106250 30.000 230 131 7 56 272 48 260 8.64e-30 116
MsG0780041080.01.T01 MTR_5g069570 29.832 238 155 7 37 272 34 261 2.43e-29 114
MsG0780041080.01.T01 MTR_3g076610 29.787 235 141 4 56 272 46 274 7.87e-29 113
MsG0780041080.01.T01 MTR_4g104760 28.700 223 150 2 56 272 47 266 9.06e-28 110
MsG0780041080.01.T01 MTR_3g082430 30.894 246 155 9 31 272 10 244 2.31e-27 108
MsG0780041080.01.T01 MTR_1g013100 29.323 266 159 10 20 272 11 260 3.01e-27 108
MsG0780041080.01.T01 MTR_5g096570 27.854 219 145 4 57 272 1208 1416 3.84e-25 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041080.01.T01 AT2G27770 43.077 325 158 11 1 304 1 319 9.77e-74 229
MsG0780041080.01.T01 AT2G36470 37.681 345 155 9 1 304 1 326 1.92e-63 203
MsG0780041080.01.T01 AT3G04860 33.981 309 179 10 1 305 1 288 1.14e-48 164
MsG0780041080.01.T01 AT5G28150 33.882 304 176 9 1 300 1 283 1.35e-46 159
MsG0780041080.01.T01 AT5G11000 29.474 285 148 10 38 293 33 293 4.23e-33 125
MsG0780041080.01.T01 AT4G12690 29.218 243 160 6 35 276 22 253 6.77e-32 120
MsG0780041080.01.T01 AT4G12690 29.218 243 160 6 35 276 22 253 6.77e-32 120
MsG0780041080.01.T01 AT5G48270 27.984 243 164 5 33 272 35 269 4.23e-29 114
MsG0780041080.01.T01 AT2G04220 29.333 225 150 5 35 258 26 242 4.77e-28 110
MsG0780041080.01.T01 AT3G13229 28.235 255 156 10 38 280 6 245 5.49e-25 102
MsG0780041080.01.T01 AT2G25200 25.347 288 170 9 4 272 14 275 3.01e-22 95.5

Find 42 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACATGTTATGTTTGTTTA+TGG 0.113859 7:+86239177 MsG0780041080.01.T01:CDS
CCATGATGCGAAATTCTTAA+TGG 0.156239 7:+86239027 MsG0780041080.01.T01:CDS
GGATAAGAAGATGGTGTTTA+AGG 0.193714 7:+86239198 MsG0780041080.01.T01:CDS
TTGCAGAAACAAAAGGAAAT+TGG 0.201357 7:+86239406 MsG0780041080.01.T01:CDS
ATTGAAGGGTTATTGAGTTT+TGG 0.288969 7:-86238629 None:intergenic
GAAGGACCTGAACCGGTTAA+TGG 0.294367 7:+86238914 MsG0780041080.01.T01:CDS
CTTTGTTCTGTTTCAACAAC+AGG 0.307651 7:-86238820 None:intergenic
GCCTTGACCAATGAGTTTCT+TGG 0.347604 7:-86238729 None:intergenic
TGATGTGGGATTTGCATGAT+TGG 0.365014 7:+86239287 MsG0780041080.01.T01:CDS
TCTGAATTGGGTTTATTCCT+TGG 0.378159 7:+86238962 MsG0780041080.01.T01:CDS
TAAAACCCATTAACCGGTTC+AGG 0.396705 7:-86238920 None:intergenic
GTGGTTTGAATAGTAGATTA+TGG 0.398722 7:+86239368 MsG0780041080.01.T01:CDS
AAGGACCTGAACCGGTTAAT+GGG 0.411080 7:+86238915 MsG0780041080.01.T01:CDS
AGAAGAATCGTTGAAGAACT+TGG 0.436270 7:+86238991 MsG0780041080.01.T01:CDS
CCATTAAGAATTTCGCATCA+TGG 0.449935 7:-86239027 None:intergenic
ATATAAATCAGATACTCTAA+TGG 0.450641 7:-86238579 None:intergenic
AATTTCTCATCACACTAACT+TGG 0.457736 7:+86238705 MsG0780041080.01.T01:CDS
TTTGGAATGATTGAATTTGA+AGG 0.474609 7:-86238611 None:intergenic
ATGACGTAAAACCCATTAAC+CGG 0.480234 7:-86238926 None:intergenic
GAAGAATTTGCAGAAACAAA+AGG 0.481804 7:+86239399 MsG0780041080.01.T01:CDS
GGATTCTCTTTGTTGATATG+TGG 0.485386 7:+86239427 MsG0780041080.01.T01:CDS
GTATGATGAAGGACCTGAAC+CGG 0.486574 7:+86238907 MsG0780041080.01.T01:CDS
ACTTGGACCAAGAAACTCAT+TGG 0.498976 7:+86238722 MsG0780041080.01.T01:CDS
ACGATTCTTCTTTGTCTCCA+AGG 0.499539 7:-86238979 None:intergenic
TTTGTTTATGGATAAGAAGA+TGG 0.500940 7:+86239189 MsG0780041080.01.T01:CDS
TTTGAATAGTAGATTATGGT+TGG 0.508048 7:+86239372 MsG0780041080.01.T01:CDS
AAAGATAGAATCAATCAGGC+AGG 0.510778 7:-86239460 None:intergenic
TTTGGTTGTGGATATGATGT+GGG 0.513802 7:+86239273 MsG0780041080.01.T01:CDS
AAGTGTGGAGTAGAAGAAGA+AGG 0.514583 7:+86239139 MsG0780041080.01.T01:CDS
GAAACAGAATCAATATTACT+AGG 0.527556 7:-86239319 None:intergenic
GTTTGGTTGTGGATATGATG+TGG 0.546002 7:+86239272 MsG0780041080.01.T01:CDS
CACGATATATTGATTAAGTG+TGG 0.553076 7:+86239124 MsG0780041080.01.T01:CDS
TTCTGTTTCAACAACAGGGG+AGG 0.562675 7:-86238815 None:intergenic
TCTGATGCTAAGTATGATGA+AGG 0.568147 7:+86238896 MsG0780041080.01.T01:CDS
ACCAAGAAACTCATTGGTCA+AGG 0.578820 7:+86238728 MsG0780041080.01.T01:CDS
TTCAAGTGTTTATGCAACAA+AGG 0.579019 7:+86239075 MsG0780041080.01.T01:CDS
TTAAGGTGAAGAGATTGAAG+TGG 0.600677 7:+86239215 MsG0780041080.01.T01:CDS
TTTATGTTTAGGACAAGGAG+TGG 0.606906 7:+86239349 MsG0780041080.01.T01:CDS
TTTGTTCTGTTTCAACAACA+GGG 0.613037 7:-86238819 None:intergenic
AAGATAGAATCAATCAGGCA+GGG 0.625244 7:-86239459 None:intergenic
TTGTTCTGTTTCAACAACAG+GGG 0.641999 7:-86238818 None:intergenic
CTGTTGTTGAAACAGAACAA+AGG 0.691667 7:+86238821 MsG0780041080.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAAATCAGATACTCTAA+TGG - Chr7:86238582-86238601 None:intergenic 20.0%
!!! ATTTTGAAGTCAAAGTTTTA+TGG + Chr7:86238867-86238886 MsG0780041080.01.T01:CDS 20.0%
!!! TAACTGAATTTTGAATTGAA+GGG - Chr7:86238646-86238665 None:intergenic 20.0%
!!! TTTTGAAGTCAAAGTTTTAT+GGG + Chr7:86238868-86238887 MsG0780041080.01.T01:CDS 20.0%
! GAAACAGAATCAATATTACT+AGG - Chr7:86239322-86239341 None:intergenic 25.0%
! TCACATGTTATGTTTGTTTA+TGG + Chr7:86239177-86239196 MsG0780041080.01.T01:CDS 25.0%
! TTTGAATAGTAGATTATGGT+TGG + Chr7:86239372-86239391 MsG0780041080.01.T01:CDS 25.0%
! TTTGTTTATGGATAAGAAGA+TGG + Chr7:86239189-86239208 MsG0780041080.01.T01:CDS 25.0%
!! AATCAAACAATTTTTGTGGA+TGG + Chr7:86239250-86239269 MsG0780041080.01.T01:CDS 25.0%
!! CATGTTTTCTCAAAATTCAA+TGG + Chr7:86238788-86238807 MsG0780041080.01.T01:CDS 25.0%
!! GTAACTGAATTTTGAATTGA+AGG - Chr7:86238647-86238666 None:intergenic 25.0%
!! TTTGGAATGATTGAATTTGA+AGG - Chr7:86238614-86238633 None:intergenic 25.0%
!!! CATTGAATTTTGAGAAAACA+TGG - Chr7:86238790-86238809 None:intergenic 25.0%
!!! TTTCTGCTGTTTTTATGTTT+AGG + Chr7:86239338-86239357 MsG0780041080.01.T01:CDS 25.0%
AATTTCTCATCACACTAACT+TGG + Chr7:86238705-86238724 MsG0780041080.01.T01:CDS 30.0%
GAAGAATTTGCAGAAACAAA+AGG + Chr7:86239399-86239418 MsG0780041080.01.T01:CDS 30.0%
GTGGTTTGAATAGTAGATTA+TGG + Chr7:86239368-86239387 MsG0780041080.01.T01:CDS 30.0%
TTCAAGTGTTTATGCAACAA+AGG + Chr7:86239075-86239094 MsG0780041080.01.T01:CDS 30.0%
TTGCAGAAACAAAAGGAAAT+TGG + Chr7:86239406-86239425 MsG0780041080.01.T01:CDS 30.0%
TTTGTTCTGTTTCAACAACA+GGG - Chr7:86238822-86238841 None:intergenic 30.0%
! AACAATTTTTGTGGATGGTT+TGG + Chr7:86239255-86239274 MsG0780041080.01.T01:CDS 30.0%
! ATTGAAGGGTTATTGAGTTT+TGG - Chr7:86238632-86238651 None:intergenic 30.0%
! CACGATATATTGATTAAGTG+TGG + Chr7:86239124-86239143 MsG0780041080.01.T01:CDS 30.0%
! TCAAGAAGTGAAAGATTTTC+TGG + Chr7:86239052-86239071 MsG0780041080.01.T01:CDS 30.0%
! TGGGAATCAAACAATTTTTG+TGG + Chr7:86239246-86239265 MsG0780041080.01.T01:CDS 30.0%
!!! CTGTTTTTATGTTTAGGACA+AGG + Chr7:86239344-86239363 MsG0780041080.01.T01:CDS 30.0%
AGAAGAATCGTTGAAGAACT+TGG + Chr7:86238991-86239010 MsG0780041080.01.T01:CDS 35.0%
ATGACGTAAAACCCATTAAC+CGG - Chr7:86238929-86238948 None:intergenic 35.0%
CCATGATGCGAAATTCTTAA+TGG + Chr7:86239027-86239046 MsG0780041080.01.T01:CDS 35.0%
CCATTAAGAATTTCGCATCA+TGG - Chr7:86239030-86239049 None:intergenic 35.0%
CTGTTGTTGAAACAGAACAA+AGG + Chr7:86238821-86238840 MsG0780041080.01.T01:CDS 35.0%
CTTTGTTCTGTTTCAACAAC+AGG - Chr7:86238823-86238842 None:intergenic 35.0%
GGATAAGAAGATGGTGTTTA+AGG + Chr7:86239198-86239217 MsG0780041080.01.T01:CDS 35.0%
GGATTCTCTTTGTTGATATG+TGG + Chr7:86239427-86239446 MsG0780041080.01.T01:CDS 35.0%
TCTGAATTGGGTTTATTCCT+TGG + Chr7:86238962-86238981 MsG0780041080.01.T01:CDS 35.0%
TCTGATGCTAAGTATGATGA+AGG + Chr7:86238896-86238915 MsG0780041080.01.T01:CDS 35.0%
TTAAGGTGAAGAGATTGAAG+TGG + Chr7:86239215-86239234 MsG0780041080.01.T01:CDS 35.0%
TTGTTCTGTTTCAACAACAG+GGG - Chr7:86238821-86238840 None:intergenic 35.0%
TTTGGTTGTGGATATGATGT+GGG + Chr7:86239273-86239292 MsG0780041080.01.T01:CDS 35.0%
! AGATTGAAGTGGAATTTTCG+TGG + Chr7:86239226-86239245 MsG0780041080.01.T01:CDS 35.0%
! GATTGAAGTGGAATTTTCGT+GGG + Chr7:86239227-86239246 MsG0780041080.01.T01:CDS 35.0%
! TTTATGTTTAGGACAAGGAG+TGG + Chr7:86239349-86239368 MsG0780041080.01.T01:CDS 35.0%
!! GTTTTGGTGAACTCTGAATT+GGG + Chr7:86238950-86238969 MsG0780041080.01.T01:CDS 35.0%
!!! AAGGCAAAGTTTTCTGAAAC+AGG + Chr7:86239094-86239113 MsG0780041080.01.T01:CDS 35.0%
AAGTGTGGAGTAGAAGAAGA+AGG + Chr7:86239139-86239158 MsG0780041080.01.T01:CDS 40.0%
ACCAAGAAACTCATTGGTCA+AGG + Chr7:86238728-86238747 MsG0780041080.01.T01:CDS 40.0%
ACGATTCTTCTTTGTCTCCA+AGG - Chr7:86238982-86239001 None:intergenic 40.0%
ACTTGGACCAAGAAACTCAT+TGG + Chr7:86238722-86238741 MsG0780041080.01.T01:CDS 40.0%
GTTTGGTTGTGGATATGATG+TGG + Chr7:86239272-86239291 MsG0780041080.01.T01:CDS 40.0%
TGATGTGGGATTTGCATGAT+TGG + Chr7:86239287-86239306 MsG0780041080.01.T01:CDS 40.0%
! TAAAACCCATTAACCGGTTC+AGG - Chr7:86238923-86238942 None:intergenic 40.0%
! TGGGTTTTACGTCATGGTTT+TGG + Chr7:86238934-86238953 MsG0780041080.01.T01:CDS 40.0%
!! GGTTTTGGTGAACTCTGAAT+TGG + Chr7:86238949-86238968 MsG0780041080.01.T01:CDS 40.0%
!!! GGTTAATGGGTTTTACGTCA+TGG + Chr7:86238928-86238947 MsG0780041080.01.T01:CDS 40.0%
!!! TTTTGTGGATGGTTTGGTTG+TGG + Chr7:86239261-86239280 MsG0780041080.01.T01:CDS 40.0%
AAGGACCTGAACCGGTTAAT+GGG + Chr7:86238915-86238934 MsG0780041080.01.T01:CDS 45.0%
GCCTTGACCAATGAGTTTCT+TGG - Chr7:86238732-86238751 None:intergenic 45.0%
GTATGATGAAGGACCTGAAC+CGG + Chr7:86238907-86238926 MsG0780041080.01.T01:CDS 45.0%
TTCTGTTTCAACAACAGGGG+AGG - Chr7:86238818-86238837 None:intergenic 45.0%
GAAGGACCTGAACCGGTTAA+TGG + Chr7:86238914-86238933 MsG0780041080.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr7 gene 86238545 86239468 86238545 ID=MsG0780041080.01;Name=MsG0780041080.01
Chr7 mRNA 86238545 86239468 86238545 ID=MsG0780041080.01.T01;Parent=MsG0780041080.01;Name=MsG0780041080.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|307
Chr7 exon 86238545 86239468 86238545 ID=MsG0780041080.01.T01:exon:19639;Parent=MsG0780041080.01.T01
Chr7 CDS 86238545 86239468 86238545 ID=MsG0780041080.01.T01:cds;Parent=MsG0780041080.01.T01
Gene Sequence

>MsG0780041080.01.T01

ATGAGAGGCATAACATCTTGTTACAATGAACATGCCATTAGAGTATCTGATTTATATTGTTCAAGACCTTCAAATTCAATCATTCCAAAACTCAATAACCCTTCAATTCAAAATTCAGTTACTTGCATATACAAACTCAACTTTTTATCAATTCAGCAACAATTTCTCATCACACTAACTTGGACCAAGAAACTCATTGGTCAAGGCTTCATCATAAACATCACTTCTGATTCTGATTATGTCCATGTTTTCTCAAAATTCAATGGAAATCCTCCCCTGTTGTTGAAACAGAACAAAGGCACTGATACGTTTCAATATCAAAATTTTGAAGTCAAAGTTTTATGGGATCTTTCTGATGCTAAGTATGATGAAGGACCTGAACCGGTTAATGGGTTTTACGTCATGGTTTTGGTGAACTCTGAATTGGGTTTATTCCTTGGAGACAAAGAAGAATCGTTGAAGAACTTGGAAGATAAGAAAAACCATGATGCGAAATTCTTAATGGTTTCAAGAAGTGAAAGATTTTCTGGTTCAAGTGTTTATGCAACAAAGGCAAAGTTTTCTGAAACAGGAATTTCACACGATATATTGATTAAGTGTGGAGTAGAAGAAGAAGGATCAACATCAAAGAGTCACATGTTATGTTTGTTTATGGATAAGAAGATGGTGTTTAAGGTGAAGAGATTGAAGTGGAATTTTCGTGGGAATCAAACAATTTTTGTGGATGGTTTGGTTGTGGATATGATGTGGGATTTGCATGATTGGATTTTCAATCCTAGTAATATTGATTCTGTTTCTGCTGTTTTTATGTTTAGGACAAGGAGTGGTTTGAATAGTAGATTATGGTTGGAAGAGAAGAATTTGCAGAAACAAAAGGAAATTGGATTCTCTTTGTTGATATGTGGATGTAAGAACCCTGCCTGA

Protein sequence

>MsG0780041080.01.T01

MRGITSCYNEHAIRVSDLYCSRPSNSIIPKLNNPSIQNSVTCIYKLNFLSIQQQFLITLTWTKKLIGQGFIINITSDSDYVHVFSKFNGNPPLLLKQNKGTDTFQYQNFEVKVLWDLSDAKYDEGPEPVNGFYVMVLVNSELGLFLGDKEESLKNLEDKKNHDAKFLMVSRSERFSGSSVYATKAKFSETGISHDILIKCGVEEEGSTSKSHMLCLFMDKKMVFKVKRLKWNFRGNQTIFVDGLVVDMMWDLHDWIFNPSNIDSVSAVFMFRTRSGLNSRLWLEEKNLQKQKEIGFSLLICGCKNPA*