Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000097.01.T01 | XP_013448778.1 | 97.297 | 74 | 2 | 0 | 1 | 74 | 20 | 93 | 4.73E-45 | 153 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000097.01.T01 | Q9FKC0 | 93.243 | 74 | 5 | 0 | 1 | 74 | 20 | 93 | 4.10E-46 | 148 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000097.01.T01 | B7FGF9 | 97.297 | 74 | 2 | 0 | 1 | 74 | 20 | 93 | 2.26e-45 | 153 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048938.01 | MsG0180000097.01 | -0.809717 | 1.648321e-50 | 9.750029e-48 |
| MsG0180000008.01 | MsG0180000097.01 | -0.816814 | 4.553696e-52 | 3.260047e-49 |
| MsG0180000097.01 | MsG0180000151.01 | -0.834506 | 2.881487e-56 | 3.426754e-53 |
| MsG0180000097.01 | MsG0180000221.01 | -0.848790 | 4.897599e-60 | 9.091368e-57 |
| MsG0180000097.01 | MsG0180000280.01 | 0.822197 | 2.693865e-53 | 2.239230e-50 |
| MsG0180000097.01 | MsG0180001119.01 | -0.803902 | 2.792486e-49 | 1.420201e-46 |
| MsG0180000097.01 | MsG0180003253.01 | -0.800298 | 1.538995e-48 | 7.140786e-46 |
| MsG0180000097.01 | MsG0180004025.01 | -0.825482 | 4.577534e-54 | 4.177338e-51 |
| MsG0180000097.01 | MsG0180004085.01 | -0.800690 | 1.280618e-48 | 6.000987e-46 |
| MsG0180000097.01 | MsG0180004112.01 | -0.839414 | 1.607248e-57 | 2.218032e-54 |
| MsG0180000097.01 | MsG0180005457.01 | -0.844189 | 8.820160e-59 | 1.412992e-55 |
| MsG0180000097.01 | MsG0180005639.01 | -0.835129 | 2.007772e-56 | 2.432365e-53 |
| MsG0180000097.01 | MsG0180005881.01 | 0.810571 | 1.078675e-50 | 6.525076e-48 |
| MsG0180000097.01 | MsG0180006054.01 | -0.836587 | 8.576316e-57 | 1.085546e-53 |
| MsG0180000097.01 | MsG0280006669.01 | -0.805103 | 1.569082e-49 | 8.230099e-47 |
| MsG0180000097.01 | MsG0280006753.01 | 0.834653 | 2.645955e-56 | 3.160113e-53 |
| MsG0180000097.01 | MsG0280007864.01 | 0.869699 | 2.545629e-66 | 9.642612e-63 |
| MsG0180000097.01 | MsG0280008210.01 | -0.805678 | 1.189016e-49 | 6.329544e-47 |
| MsG0180000097.01 | MsG0280009551.01 | -0.800803 | 1.214194e-48 | 5.706058e-46 |
| MsG0180000097.01 | MsG0280010253.01 | -0.801884 | 7.293909e-49 | 3.523047e-46 |
| MsG0180000097.01 | MsG0280011272.01 | 0.833976 | 3.913594e-56 | 4.580893e-53 |
| MsG0180000097.01 | MsG0280011404.01 | -0.813901 | 2.024647e-51 | 1.338886e-48 |
| MsG0180000097.01 | MsG0380012005.01 | -0.803679 | 3.106565e-49 | 1.570792e-46 |
| MsG0180000097.01 | MsG0380012249.01 | -0.825585 | 4.326754e-54 | 3.960232e-51 |
| MsG0180000097.01 | MsG0380012279.01 | 0.802734 | 4.874977e-49 | 2.406144e-46 |
| MsG0180000097.01 | MsG0380012357.01 | -0.824476 | 7.907993e-54 | 7.011815e-51 |
| MsG0180000097.01 | MsG0380014727.01 | -0.830983 | 2.159008e-55 | 2.311564e-52 |
| MsG0180000097.01 | MsG0380014866.01 | -0.803692 | 3.087458e-49 | 1.561673e-46 |
| MsG0180000097.01 | MsG0380015847.01 | 0.804892 | 1.736316e-49 | 9.059142e-47 |
| MsG0180000097.01 | MsG0380016182.01 | -0.847549 | 1.078286e-59 | 1.923493e-56 |
| MsG0180000097.01 | MsG0380016201.01 | 0.899512 | 1.842280e-77 | 2.285836e-73 |
| MsG0180000097.01 | MsG0380016527.01 | -0.822878 | 1.871877e-53 | 1.586472e-50 |
| MsG0180000097.01 | MsG0380016805.01 | 0.813913 | 2.012201e-51 | 1.331031e-48 |
| MsG0180000097.01 | MsG0380017220.01 | -0.811578 | 6.528366e-51 | 4.055928e-48 |
| MsG0180000097.01 | MsG0480018226.01 | -0.825051 | 5.785921e-54 | 5.216333e-51 |
| MsG0180000097.01 | MsG0480018236.01 | -0.804707 | 1.898579e-49 | 9.857367e-47 |
| MsG0180000097.01 | MsG0480019384.01 | -0.835577 | 1.547190e-56 | 1.899800e-53 |
| MsG0180000097.01 | MsG0480019546.01 | -0.808304 | 3.307479e-50 | 1.884506e-47 |
| MsG0180000097.01 | MsG0480020322.01 | -0.864011 | 1.652668e-64 | 5.111360e-61 |
| MsG0180000097.01 | MsG0480020323.01 | -0.883418 | 4.561903e-71 | 2.889401e-67 |
| MsG0180000097.01 | MsG0480020433.01 | -0.811198 | 7.893745e-51 | 4.855398e-48 |
| MsG0180000097.01 | MsG0480021564.01 | -0.808957 | 2.399383e-50 | 1.390701e-47 |
| MsG0180000097.01 | MsG0480022066.01 | -0.835754 | 1.395950e-56 | 1.723289e-53 |
| MsG0180000097.01 | MsG0480022145.01 | -0.801760 | 7.733171e-49 | 3.723114e-46 |
| MsG0180000097.01 | MsG0480022535.01 | -0.870742 | 1.159536e-66 | 4.560012e-63 |
| MsG0180000097.01 | MsG0480022739.01 | 0.816471 | 5.437629e-52 | 3.856314e-49 |
| MsG0180000097.01 | MsG0480022802.01 | -0.825623 | 4.237176e-54 | 3.882534e-51 |
| MsG0180000097.01 | MsG0480023251.01 | -0.836497 | 9.038009e-57 | 1.140958e-53 |
| MsG0180000097.01 | MsG0580024363.01 | 0.804462 | 2.135675e-49 | 1.101808e-46 |
| MsG0180000097.01 | MsG0580024975.01 | -0.850449 | 1.685480e-60 | 3.302911e-57 |
| MsG0180000097.01 | MsG0580025226.01 | -0.804824 | 1.794282e-49 | 9.344618e-47 |
| MsG0180000097.01 | MsG0580025644.01 | -0.854040 | 1.603464e-61 | 3.535040e-58 |
| MsG0180000097.01 | MsG0580025827.01 | -0.829025 | 6.482705e-55 | 6.554286e-52 |
| MsG0180000097.01 | MsG0580025832.01 | -0.845185 | 4.755860e-59 | 7.860842e-56 |
| MsG0180000097.01 | MsG0580026031.01 | -0.823288 | 1.501769e-53 | 1.287556e-50 |
| MsG0180000097.01 | MsG0580026032.01 | -0.837179 | 6.056324e-57 | 7.804455e-54 |
| MsG0180000097.01 | MsG0580026304.01 | -0.812085 | 5.063228e-51 | 3.188714e-48 |
| MsG0180000097.01 | MsG0680030566.01 | 0.808395 | 3.163128e-50 | 1.806612e-47 |
| MsG0180000097.01 | MsG0680031376.01 | 0.837732 | 4.370536e-57 | 5.729413e-54 |
| MsG0180000097.01 | MsG0680031377.01 | 0.863016 | 3.361547e-64 | 1.004348e-60 |
| MsG0180000097.01 | MsG0680031406.01 | -0.805099 | 1.572216e-49 | 8.245611e-47 |
| MsG0180000097.01 | MsG0680031996.01 | -0.827760 | 1.309401e-54 | 1.275927e-51 |
| MsG0180000097.01 | MsG0680032058.01 | -0.811610 | 6.422230e-51 | 3.993342e-48 |
| MsG0180000097.01 | MsG0680032460.01 | 0.826477 | 2.655246e-54 | 2.492784e-51 |
| MsG0180000097.01 | MsG0680033639.01 | -0.828842 | 7.179494e-55 | 7.219728e-52 |
| MsG0180000097.01 | MsG0680034032.01 | -0.864672 | 1.027482e-64 | 3.252882e-61 |
| MsG0180000097.01 | MsG0680034402.01 | -0.814772 | 1.299347e-51 | 8.797059e-49 |
| MsG0180000097.01 | MsG0680034909.01 | 0.801690 | 7.995234e-49 | 3.842432e-46 |
| MsG0180000097.01 | MsG0680035707.01 | -0.828670 | 7.902708e-55 | 7.907936e-52 |
| MsG0180000097.01 | MsG0780036116.01 | -0.845797 | 3.246606e-59 | 5.474134e-56 |
| MsG0180000097.01 | MsG0780036509.01 | -0.801376 | 9.272061e-49 | 4.421067e-46 |
| MsG0180000097.01 | MsG0780037105.01 | -0.879289 | 1.407555e-69 | 7.589343e-66 |
| MsG0180000097.01 | MsG0780039668.01 | 0.839030 | 2.021650e-57 | 2.757072e-54 |
| MsG0180000097.01 | MsG0780040177.01 | -0.806995 | 6.275034e-50 | 3.454981e-47 |
| MsG0180000097.01 | MsG0780040518.01 | -0.810244 | 1.269335e-50 | 7.612392e-48 |
| MsG0180000097.01 | MsG0780040559.01 | 0.858394 | 8.489371e-63 | 2.163467e-59 |
| MsG0180000097.01 | MsG0780040576.01 | 0.825230 | 5.249383e-54 | 4.756666e-51 |
| MsG0180000097.01 | MsG0780041015.01 | 0.837732 | 4.370536e-57 | 5.729413e-54 |
| MsG0180000097.01 | MsG0780041080.01 | -0.816944 | 4.258453e-52 | 3.059834e-49 |
| MsG0180000097.01 | MsG0780041187.01 | -0.804486 | 2.111282e-49 | 1.089885e-46 |
| MsG0180000097.01 | MsG0780041191.01 | -0.861761 | 8.175624e-64 | 2.338169e-60 |
| MsG0180000097.01 | MsG0780041238.01 | 0.817927 | 2.558520e-52 | 1.888416e-49 |
| MsG0180000097.01 | MsG0780041251.01 | 0.814028 | 1.897591e-51 | 1.259205e-48 |
| MsG0180000097.01 | MsG0780041301.01 | -0.864741 | 9.778008e-65 | 3.103052e-61 |
| MsG0180000097.01 | MsG0880041852.01 | -0.831777 | 1.377006e-55 | 1.509676e-52 |
| MsG0180000097.01 | MsG0880042000.01 | -0.809256 | 2.070293e-50 | 1.209644e-47 |
| MsG0180000097.01 | MsG0880042435.01 | 0.808809 | 2.580015e-50 | 1.489528e-47 |
| MsG0180000097.01 | MsG0880042948.01 | -0.850313 | 1.841368e-60 | 3.592270e-57 |
| MsG0180000097.01 | MsG0880044572.01 | -0.802389 | 5.742073e-49 | 2.809402e-46 |
| MsG0180000097.01 | MsG0880045370.01 | -0.828452 | 8.920043e-55 | 8.870207e-52 |
| MsG0180000097.01 | MsG0880045386.01 | -0.854204 | 1.438211e-61 | 3.187932e-58 |
| MsG0180000097.01 | MsG0880045624.01 | -0.803151 | 3.997832e-49 | 1.994241e-46 |
| MsG0180000097.01 | MsG0880045771.01 | -0.815516 | 8.882323e-52 | 6.137326e-49 |
| MsG0180000097.01 | MsG0880045783.01 | -0.832452 | 9.378104e-56 | 1.048596e-52 |
| MsG0180000097.01 | MsG0880045926.01 | -0.804288 | 2.321117e-49 | 1.192172e-46 |
| MsG0180000097.01 | MsG0880046207.01 | -0.858457 | 8.132291e-63 | 2.076874e-59 |
| MsG0180000097.01 | MsG0880046268.01 | -0.822573 | 2.204004e-53 | 1.852033e-50 |
| MsG0180000097.01 | MsG0880046296.01 | -0.835322 | 1.794975e-56 | 2.187635e-53 |
| MsG0180000097.01 | MsG0880046780.01 | 0.823321 | 1.475216e-53 | 1.265952e-50 |
| MsG0180000097.01 | MsG0880047267.01 | -0.800246 | 1.576690e-48 | 7.305808e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000097.01.T01 | MTR_7g057770 | 97.297 | 74 | 2 | 0 | 1 | 74 | 20 | 93 | 5.72e-49 | 153 |
| MsG0180000097.01.T01 | MTR_1g023590 | 97.297 | 74 | 2 | 0 | 1 | 74 | 20 | 93 | 9.77e-49 | 152 |
| MsG0180000097.01.T01 | MTR_5g083790 | 93.243 | 74 | 5 | 0 | 1 | 74 | 20 | 93 | 5.36e-47 | 148 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000097.01.T01 | AT5G48760 | 93.243 | 74 | 5 | 0 | 1 | 74 | 20 | 93 | 4.18e-47 | 148 |
| MsG0180000097.01.T01 | AT5G48760 | 93.243 | 74 | 5 | 0 | 1 | 74 | 20 | 93 | 4.18e-47 | 148 |
| MsG0180000097.01.T01 | AT3G07110 | 89.189 | 74 | 8 | 0 | 1 | 74 | 20 | 93 | 5.17e-46 | 145 |
| MsG0180000097.01.T01 | AT3G07110 | 89.189 | 74 | 8 | 0 | 1 | 74 | 20 | 93 | 5.48e-46 | 145 |
| MsG0180000097.01.T01 | AT3G24830 | 90.541 | 74 | 7 | 0 | 1 | 74 | 20 | 93 | 1.06e-45 | 145 |
| MsG0180000097.01.T01 | AT4G13170 | 90.541 | 74 | 7 | 0 | 1 | 74 | 20 | 93 | 3.35e-45 | 144 |
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATGGTCCTATTCACTTTA+GGG | 0.192347 | 1:-1389624 | MsG0180000097.01.T01:CDS |
| CTCATGGTCCTATTCACTTT+AGG | 0.198145 | 1:-1389625 | MsG0180000097.01.T01:CDS |
| GGGCTCCCTCTAAGATCTTT+TGG | 0.279612 | 1:-1389604 | MsG0180000097.01.T01:CDS |
| TGTGAAGAAATTTGTGCTTC+AGG | 0.399041 | 1:-1389713 | MsG0180000097.01.T01:CDS |
| TGAAGTATATGAGATTTCTA+AGG | 0.407625 | 1:-1389670 | MsG0180000097.01.T01:CDS |
| GAGGGAGCCCTAAAGTGAAT+AGG | 0.470476 | 1:+1389617 | None:intergenic |
| ATATGAGATTTCTAAGGAAG+AGG | 0.501956 | 1:-1389664 | MsG0180000097.01.T01:CDS |
| GACGCCCGCCACCACATGCT+CGG | 0.519214 | 1:-1389797 | None:intergenic |
| AGTGCGCCAAAAGATCTTAG+AGG | 0.534021 | 1:+1389598 | None:intergenic |
| GTCGCAAAGGAGCTGCTCAA+TGG | 0.550130 | 1:-1389758 | MsG0180000097.01.T01:CDS |
| GAGCAGCTCCTTTGCGACAA+TGG | 0.550375 | 1:+1389763 | None:intergenic |
| ATGAATACTAAACCTTCTCA+TGG | 0.560213 | 1:-1389641 | MsG0180000097.01.T01:CDS |
| TCTTGCCTCCATTGTCGCAA+AGG | 0.579194 | 1:-1389771 | MsG0180000097.01.T01:CDS |
| GGAGCTGCTCAATGGTCAGA+AGG | 0.597676 | 1:-1389750 | MsG0180000097.01.T01:CDS |
| GTGCGCCAAAAGATCTTAGA+GGG | 0.602717 | 1:+1389599 | None:intergenic |
| TTGCGACAATGGAGGCAAGA+CGG | 0.613146 | 1:+1389774 | None:intergenic |
| CAGCTCCTTTGCGACAATGG+AGG | 0.625227 | 1:+1389766 | None:intergenic |
| GCTGCTCAATGGTCAGAAGG+TGG | 0.667003 | 1:-1389747 | MsG0180000097.01.T01:CDS |
| AAGTGAATAGGACCATGAGA+AGG | 0.669515 | 1:+1389629 | None:intergenic |
| GAAGAAATTTGTGCTTCAGG+TGG | 0.691821 | 1:-1389710 | MsG0180000097.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGAAGTATATGAGATTTCTA+AGG | - | Chr1:1389689-1389708 | MsG0180000097.01.T01:CDS | 25.0% |
| ATGAATACTAAACCTTCTCA+TGG | - | Chr1:1389718-1389737 | MsG0180000097.01.T01:CDS | 30.0% | |
| ! | ATATGAGATTTCTAAGGAAG+AGG | - | Chr1:1389695-1389714 | MsG0180000097.01.T01:CDS | 30.0% |
| TCATGGTCCTATTCACTTTA+GGG | - | Chr1:1389735-1389754 | MsG0180000097.01.T01:CDS | 35.0% | |
| TGTGAAGAAATTTGTGCTTC+AGG | - | Chr1:1389646-1389665 | MsG0180000097.01.T01:CDS | 35.0% | |
| AAGTGAATAGGACCATGAGA+AGG | + | Chr1:1389733-1389752 | None:intergenic | 40.0% | |
| CTCATGGTCCTATTCACTTT+AGG | - | Chr1:1389734-1389753 | MsG0180000097.01.T01:CDS | 40.0% | |
| GAAGAAATTTGTGCTTCAGG+TGG | - | Chr1:1389649-1389668 | MsG0180000097.01.T01:CDS | 40.0% | |
| AGTGCGCCAAAAGATCTTAG+AGG | + | Chr1:1389764-1389783 | None:intergenic | 45.0% | |
| GTGCGCCAAAAGATCTTAGA+GGG | + | Chr1:1389763-1389782 | None:intergenic | 45.0% | |
| GAGGGAGCCCTAAAGTGAAT+AGG | + | Chr1:1389745-1389764 | None:intergenic | 50.0% | |
| TCTTGCCTCCATTGTCGCAA+AGG | - | Chr1:1389588-1389607 | MsG0180000097.01.T01:CDS | 50.0% | |
| TTGCGACAATGGAGGCAAGA+CGG | + | Chr1:1389588-1389607 | None:intergenic | 50.0% | |
| ! | ATCTTTTGGCGCACTGTTCG+TGG | - | Chr1:1389769-1389788 | MsG0180000097.01.T01:CDS | 50.0% |
| ! | TCTTTTGGCGCACTGTTCGT+GGG | - | Chr1:1389770-1389789 | MsG0180000097.01.T01:CDS | 50.0% |
| !!! | GGGCTCCCTCTAAGATCTTT+TGG | - | Chr1:1389755-1389774 | MsG0180000097.01.T01:CDS | 50.0% |
| CAGCTCCTTTGCGACAATGG+AGG | + | Chr1:1389596-1389615 | None:intergenic | 55.0% | |
| GAGCAGCTCCTTTGCGACAA+TGG | + | Chr1:1389599-1389618 | None:intergenic | 55.0% | |
| GCTGCTCAATGGTCAGAAGG+TGG | - | Chr1:1389612-1389631 | MsG0180000097.01.T01:CDS | 55.0% | |
| GGAGCTGCTCAATGGTCAGA+AGG | - | Chr1:1389609-1389628 | MsG0180000097.01.T01:CDS | 55.0% | |
| GTCGCAAAGGAGCTGCTCAA+TGG | - | Chr1:1389601-1389620 | MsG0180000097.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 1389577 | 1389804 | 1389577 | ID=MsG0180000097.01;Name=MsG0180000097.01 |
| Chr1 | mRNA | 1389577 | 1389804 | 1389577 | ID=MsG0180000097.01.T01;Parent=MsG0180000097.01;Name=MsG0180000097.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|75 |
| Chr1 | exon | 1389577 | 1389804 | 1389577 | ID=MsG0180000097.01.T01:exon:7286;Parent=MsG0180000097.01.T01 |
| Chr1 | CDS | 1389577 | 1389804 | 1389577 | ID=MsG0180000097.01.T01:cds;Parent=MsG0180000097.01.T01 |
| Gene Sequence |
| Protein sequence |
