AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029149.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029149.01.T01 MTR_5g081910 100.000 54 0 0 1 54 1 54 5.33e-32 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029149.01.T01 AT2G39970 79.630 54 11 0 1 54 1 54 1.54e-12 63.5

Find 34 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAAATGTGTCAGGTTTA+TGG 0.125295 5:-92910302 MsG0580029149.01.T01:CDS
TTCAGTGTCAACGTTGCGTT+TGG 0.316611 5:+92910731 None:intergenic
ACAAAGATCATGTGATTGTT+TGG 0.341999 5:-92910271 MsG0580029149.01.T01:CDS
GAGGGATGGGAGAGGTTGTA+TGG 0.373207 5:-92909641 MsG0580029149.01.T01:CDS
ATGTCGGACGCTTTGATCAA+TGG 0.399669 5:-92910546 MsG0580029149.01.T01:CDS
AGATTCTTATTGCCCTTCTT+CGG 0.413495 5:+92910335 None:intergenic
TATTGTATGAAGAGAGAAAA+GGG 0.427241 5:+92910647 None:intergenic
GTGATTGTTTGGTTAATGTT+TGG 0.430938 5:-92910260 MsG0580029149.01.T01:CDS
AAGAAGGGCAATAAGAATCT+TGG 0.440862 5:-92910332 MsG0580029149.01.T01:CDS
ATCAATGGATTGGCTGGAGC+TGG 0.443957 5:-92910531 MsG0580029149.01.T01:CDS
GGATTGGCTGGAGCTGGTGG+AGG 0.464570 5:-92910525 MsG0580029149.01.T01:CDS
GCTTTGATCAATGGATTGGC+TGG 0.469248 5:-92910537 MsG0580029149.01.T01:CDS
GGCTTGGCGCCATCACTTGT+CGG 0.475838 5:-92909617 MsG0580029149.01.T01:CDS
GGACGCTTTGATCAATGGAT+TGG 0.498413 5:-92910541 MsG0580029149.01.T01:CDS
AACTGATCGTGATCCGAAGA+AGG 0.505218 5:-92910348 MsG0580029149.01.T01:CDS
GAAAATCATACAGTTTGAAG+AGG 0.510497 5:+92910477 None:intergenic
GGAGAGGTTGTATGGAGGCT+TGG 0.511587 5:-92909633 MsG0580029149.01.T01:CDS
AATGGATTGGCTGGAGCTGG+TGG 0.518922 5:-92910528 MsG0580029149.01.T01:CDS
GGTTGTGAAACAAGAGGGAT+GGG 0.523770 5:-92909654 MsG0580029149.01.T01:CDS
TGTCGGTACAGCTACATCTC+AGG 0.524615 5:-92909600 MsG0580029149.01.T01:CDS
TTTGAAGTAGTTGTTGTACA+TGG 0.529730 5:+92910691 None:intergenic
TCAGTGTCAACGTTGCGTTT+GGG 0.538749 5:+92910732 None:intergenic
AGGTTGTGAAACAAGAGGGA+TGG 0.541422 5:-92909655 MsG0580029149.01.T01:CDS
GTATTGTATGAAGAGAGAAA+AGG 0.542384 5:+92910646 None:intergenic
GGATGGGAGAGGTTGTATGG+AGG 0.563808 5:-92909638 MsG0580029149.01.T01:CDS
TGAAACAAGAGGGATGGGAG+AGG 0.572625 5:-92909649 MsG0580029149.01.T01:CDS
GATTGGCTGGAGCTGGTGGA+GGG 0.575191 5:-92910524 MsG0580029149.01.T01:CDS
AAAATCATACAGTTTGAAGA+GGG 0.611736 5:+92910478 None:intergenic
ACTGATCGTGATCCGAAGAA+GGG 0.613764 5:-92910347 MsG0580029149.01.T01:CDS
TTGGTTAATGTTTGGCCGTG+AGG 0.638927 5:-92910252 MsG0580029149.01.T01:CDS
GTAGCTGTACCGACAAGTGA+TGG 0.655326 5:+92909608 None:intergenic
ATTGTAGGTTGTGAAACAAG+AGG 0.675879 5:-92909660 MsG0580029149.01.T01:intron
AAAACATCCTCAAAACATGT+CGG 0.676069 5:-92910562 MsG0580029149.01.T01:exon
TTGTAGGTTGTGAAACAAGA+GGG 0.697405 5:-92909659 MsG0580029149.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAAAATCAATTTG+AGG + Chr5:92909688-92909707 None:intergenic 10.0%
!!! TCTTTTATTTTTGTTATATT+GGG - Chr5:92910564-92910583 MsG0580029149.01.T01:exon 10.0%
!!! TTCTTTTATTTTTGTTATAT+TGG - Chr5:92910563-92910582 MsG0580029149.01.T01:exon 10.0%
!! AGTTGAACAATAAATTTAAG+TGG + Chr5:92910347-92910366 None:intergenic 20.0%
!! GGAAAAAAAAAATCAATTCT+AGG + Chr5:92910326-92910345 None:intergenic 20.0%
!!! GAAATTTGATAATGGTTTTT+TGG - Chr5:92910517-92910536 MsG0580029149.01.T01:CDS 20.0%
! AAAATCATACAGTTTGAAGA+GGG + Chr5:92909806-92909825 None:intergenic 25.0%
! AAGGTAAAATGATCTATGTT+TGG + Chr5:92910389-92910408 None:intergenic 25.0%
! ACAAAACATACACAGAAAAA+CGG - Chr5:92910495-92910514 MsG0580029149.01.T01:CDS 25.0%
! GAAAAACGGAAATTTGATAA+TGG - Chr5:92910509-92910528 MsG0580029149.01.T01:CDS 25.0%
! TATTGTATGAAGAGAGAAAA+GGG + Chr5:92909637-92909656 None:intergenic 25.0%
! TTAAGTAGAATCAATTCTAG+AGG - Chr5:92910184-92910203 MsG0580029149.01.T01:intron 25.0%
!! AATTCTGCACATATCAATTT+TGG - Chr5:92910455-92910474 MsG0580029149.01.T01:intron 25.0%
!! AGTTTCAATCTTTTCTCAAT+TGG - Chr5:92909834-92909853 MsG0580029149.01.T01:intron 25.0%
!! GTTTCAATCTTTTCTCAATT+GGG - Chr5:92909835-92909854 MsG0580029149.01.T01:intron 25.0%
!! TCAAAATCAAATTTTCCTCA+CGG + Chr5:92910047-92910066 None:intergenic 25.0%
!!! TGTATTTTTTGTTCCACTTT+TGG - Chr5:92910214-92910233 MsG0580029149.01.T01:CDS 25.0%
!!! TTGATTTTGAGTCAATTGTT+TGG - Chr5:92910057-92910076 MsG0580029149.01.T01:intron 25.0%
AAAAACACTGAGCTAAAATG+AGG + Chr5:92910710-92910729 None:intergenic 30.0%
AAAACATCCTCAAAACATGT+CGG - Chr5:92909719-92909738 MsG0580029149.01.T01:intron 30.0%
ACAAAGATCATGTGATTGTT+TGG - Chr5:92910010-92910029 MsG0580029149.01.T01:intron 30.0%
GAAAATCATACAGTTTGAAG+AGG + Chr5:92909807-92909826 None:intergenic 30.0%
GTATTGTATGAAGAGAGAAA+AGG + Chr5:92909638-92909657 None:intergenic 30.0%
GTGATTGTTTGGTTAATGTT+TGG - Chr5:92910021-92910040 MsG0580029149.01.T01:intron 30.0%
TACTTGAAGCTAGAAATCAT+CGG - Chr5:92910293-92910312 MsG0580029149.01.T01:CDS 30.0%
TCAAGTAGAATCAATTCTAG+TGG + Chr5:92910280-92910299 None:intergenic 30.0%
! AACTTTTCAACAAATGTGTC+AGG - Chr5:92909972-92909991 MsG0580029149.01.T01:intron 30.0%
! AAGGGTAGTTTTCAATTACT+TGG + Chr5:92909619-92909638 None:intergenic 30.0%
! AGGGTAGTTTTCAATTACTT+GGG + Chr5:92909618-92909637 None:intergenic 30.0%
! GATTAGTTGTTTAGTGATTG+TGG - Chr5:92910586-92910605 MsG0580029149.01.T01:five_prime_UTR 30.0%
! GTTTTTGTGTTGTGATCTAT+AGG - Chr5:92909894-92909913 MsG0580029149.01.T01:intron 30.0%
! TACACCTCAAGAATCAATTT+TGG + Chr5:92910245-92910264 None:intergenic 30.0%
! TTTGAAGTAGTTGTTGTACA+TGG + Chr5:92909593-92909612 None:intergenic 30.0%
! TTTTTGGACTGAATGAAAGT+TGG - Chr5:92910533-92910552 MsG0580029149.01.T01:CDS 30.0%
!!! ATTTTTTGTTCCACTTTTGG+AGG - Chr5:92910217-92910236 MsG0580029149.01.T01:CDS 30.0%
!!! GAGGATGTTTTATTTTTCTC+AGG + Chr5:92909669-92909688 None:intergenic 30.0%
!!! TCATTTTAGCTCAGTGTTTT+TGG - Chr5:92910709-92910728 MsG0580029149.01.T01:five_prime_UTR 30.0%
AAGAAGGGCAATAAGAATCT+TGG - Chr5:92909949-92909968 MsG0580029149.01.T01:intron 35.0%
AGATTCTTATTGCCCTTCTT+CGG + Chr5:92909949-92909968 None:intergenic 35.0%
ATTGTAGGTTGTGAAACAAG+AGG - Chr5:92910621-92910640 MsG0580029149.01.T01:five_prime_UTR 35.0%
CAACAAATGTGTCAGGTTTA+TGG - Chr5:92909979-92909998 MsG0580029149.01.T01:intron 35.0%
TGGTTATGTCAATGCATTGT+AGG - Chr5:92910606-92910625 MsG0580029149.01.T01:five_prime_UTR 35.0%
TTGTAGGTTGTGAAACAAGA+GGG - Chr5:92910622-92910641 MsG0580029149.01.T01:five_prime_UTR 35.0%
! CCTTCAACTCAATTTTAGAC+AGG - Chr5:92910405-92910424 MsG0580029149.01.T01:intron 35.0%
! GTATGTTTTGTCTCCACTTT+TGG + Chr5:92910486-92910505 None:intergenic 35.0%
!! CCTGTCTAAAATTGAGTTGA+AGG + Chr5:92910408-92910427 None:intergenic 35.0%
! GGAGCCAAAATTGATTCTTG+AGG - Chr5:92910238-92910257 MsG0580029149.01.T01:CDS 40.0%
!! TGTTTTGTCTCCACTTTTGG+AGG + Chr5:92910483-92910502 None:intergenic 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG - Chr5:92910470-92910489 MsG0580029149.01.T01:intron 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG + Chr5:92910489-92910470 None:intergenic 40.0%
AACTGATCGTGATCCGAAGA+AGG - Chr5:92909933-92909952 MsG0580029149.01.T01:intron 45.0%
ACTGATCGTGATCCGAAGAA+GGG - Chr5:92909934-92909953 MsG0580029149.01.T01:intron 45.0%
AGGTTGTGAAACAAGAGGGA+TGG - Chr5:92910626-92910645 MsG0580029149.01.T01:five_prime_UTR 45.0%
ATGTCGGACGCTTTGATCAA+TGG - Chr5:92909735-92909754 MsG0580029149.01.T01:intron 45.0%
GGACGCTTTGATCAATGGAT+TGG - Chr5:92909740-92909759 MsG0580029149.01.T01:intron 45.0%
GGTTGTGAAACAAGAGGGAT+GGG - Chr5:92910627-92910646 MsG0580029149.01.T01:five_prime_UTR 45.0%
TTGGTTAATGTTTGGCCGTG+AGG - Chr5:92910029-92910048 MsG0580029149.01.T01:intron 45.0%
!! AAAGCGTCCGACATGTTTTG+AGG + Chr5:92909729-92909748 None:intergenic 45.0%
!! GCTTTGATCAATGGATTGGC+TGG - Chr5:92909744-92909763 MsG0580029149.01.T01:intron 45.0%
TGAAACAAGAGGGATGGGAG+AGG - Chr5:92910632-92910651 MsG0580029149.01.T01:five_prime_UTR 50.0%
TGTCGGTACAGCTACATCTC+AGG - Chr5:92910681-92910700 MsG0580029149.01.T01:five_prime_UTR 50.0%
! GTAGCTGTACCGACAAGTGA+TGG + Chr5:92910676-92910695 None:intergenic 50.0%
!! ATCAATGGATTGGCTGGAGC+TGG - Chr5:92909750-92909769 MsG0580029149.01.T01:intron 50.0%
GAGGGATGGGAGAGGTTGTA+TGG - Chr5:92910640-92910659 MsG0580029149.01.T01:five_prime_UTR 55.0%
GGAGAGGTTGTATGGAGGCT+TGG - Chr5:92910648-92910667 MsG0580029149.01.T01:five_prime_UTR 55.0%
GGATGGGAGAGGTTGTATGG+AGG - Chr5:92910643-92910662 MsG0580029149.01.T01:five_prime_UTR 55.0%
!! AATGGATTGGCTGGAGCTGG+TGG - Chr5:92909753-92909772 MsG0580029149.01.T01:intron 55.0%
GGCTTGGCGCCATCACTTGT+CGG - Chr5:92910664-92910683 MsG0580029149.01.T01:five_prime_UTR 60.0%
! GATTGGCTGGAGCTGGTGGA+GGG - Chr5:92909757-92909776 MsG0580029149.01.T01:intron 60.0%
!! GGATTGGCTGGAGCTGGTGG+AGG - Chr5:92909756-92909775 MsG0580029149.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 92909551 92910752 92909551 ID=MsG0580029149.01;Name=MsG0580029149.01
Chr5 mRNA 92909551 92910752 92909551 ID=MsG0580029149.01.T01;Parent=MsG0580029149.01;Name=MsG0580029149.01.T01;_AED=0.49;_eAED=0.63;_QI=184|0.5|0.33|1|1|1|3|0|133
Chr5 exon 92909551 92909675 92909551 ID=MsG0580029149.01.T01:exon:17942;Parent=MsG0580029149.01.T01
Chr5 exon 92910192 92910387 92910192 ID=MsG0580029149.01.T01:exon:17941;Parent=MsG0580029149.01.T01
Chr5 exon 92910488 92910752 92910488 ID=MsG0580029149.01.T01:exon:17940;Parent=MsG0580029149.01.T01
Chr5 five_prime_UTR 92910569 92910752 92910569 ID=MsG0580029149.01.T01:five_prime_utr;Parent=MsG0580029149.01.T01
Chr5 CDS 92910488 92910568 92910488 ID=MsG0580029149.01.T01:cds;Parent=MsG0580029149.01.T01
Chr5 CDS 92910192 92910387 92910192 ID=MsG0580029149.01.T01:cds;Parent=MsG0580029149.01.T01
Chr5 CDS 92909551 92909675 92909551 ID=MsG0580029149.01.T01:cds;Parent=MsG0580029149.01.T01
Gene Sequence

>MsG0580029149.01.T01

ATGTCGGACGCTTTGATCAATGGATTGGCTGGAGCTGGTGGAGGGATCATTGCTCAACTTATTACATACCCTCTTCAAACTGTGAATACTCGTCAACAAACTGATCGTGATCCGAAGAAGGGCAATAAGAATCTTGGAACTTTTCAACAAATGTGTCAGGTTTATGGCTTTTTTAACAAAGATCATGTGATTGTTTGGTTAATGTTTGGCCGTGAGGAAAATTTGATTTTGAGTCAATTGTTTGGTAAAAGTGATTTATGTTTGAATACATTTAAATGTTGTGAAACAAGAGGGATGGGAGAGGTTGTATGGAGGCTTGGCGCCATCACTTGTCGGTACAGCTACATCTCAGGTGACCTCATTTTAGCTCAGTGTTTTTGGAGATATGTGATTTTTGAGTAA

Protein sequence

>MsG0580029149.01.T01

MSDALINGLAGAGGGIIAQLITYPLQTVNTRQQTDRDPKKGNKNLGTFQQMCQVYGFFNKDHVIVWLMFGREENLILSQLFGKSDLCLNTFKCCETRGMGEVVWRLGAITCRYSYISGDLILAQCFWRYVIFE*