AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005267.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005267.01.T01 MTR_1g095900 49.524 210 9 5 1 138 1 185 3.82e-50 158
MsG0180005267.01.T01 MTR_1g095890 44.076 211 20 4 1 138 1 186 1.71e-42 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005267.01.T01 AT2G35810 43.382 136 25 4 25 137 92 198 3.38e-26 97.8
MsG0180005267.01.T01 AT2G35810 43.382 136 25 4 25 137 92 198 3.38e-26 97.8
MsG0180005267.01.T01 AT2G35830 43.609 133 30 4 25 137 73 180 7.80e-25 94.0
MsG0180005267.01.T01 AT2G35830 43.609 133 30 4 25 137 78 185 7.81e-25 94.0
MsG0180005267.01.T01 AT2G35820 40.458 131 35 4 25 137 78 183 1.73e-21 85.5
MsG0180005267.01.T01 AT2G35820 40.458 131 35 4 25 137 168 273 4.90e-21 86.3

Find 32 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTAATGTTTGGTATCT+TGG 0.305200 1:+89373697 MsG0180005267.01.T01:CDS
CGGTGAAGCTTGACGAATTT+GGG 0.309643 1:-89373855 None:intergenic
GCGGTGAAGCTTGACGAATT+TGG 0.316031 1:-89373856 None:intergenic
CGTGAAGGTGGTGACAATTA+AGG 0.355574 1:+89372486 MsG0180005267.01.T01:CDS
AGAGCATGTCAAATGAATTC+AGG 0.383024 1:-89374001 None:intergenic
GGTCCATTGGTGGTAATGTT+TGG 0.450428 1:+89373689 MsG0180005267.01.T01:CDS
GATGTAAGATTCCATTGAAA+GGG 0.457158 1:-89373969 None:intergenic
GACTCAGTGCCTGGGTCCAT+TGG 0.465841 1:+89373676 MsG0180005267.01.T01:intron
CACCGCGGTACATGGCATGC+TGG 0.498508 1:+89373873 MsG0180005267.01.T01:CDS
TCATTTGAATCAACAACTGA+TGG 0.498895 1:-89373730 None:intergenic
TCAAGCTTCACCGCGGTACA+TGG 0.506906 1:+89373865 MsG0180005267.01.T01:CDS
TCAAAGATGACTCAGGAAAA+CGG 0.522749 1:+89373755 MsG0180005267.01.T01:CDS
TAAGGCAATCGAAGCAACTC+CGG 0.529722 1:+89372504 MsG0180005267.01.T01:CDS
CCGTAGTCTTTGAAGTTCGC+CGG 0.533633 1:-89372523 None:intergenic
ATACCAAACATTACCACCAA+TGG 0.547916 1:-89373692 None:intergenic
AGATGTAAGATTCCATTGAA+AGG 0.548712 1:-89373970 None:intergenic
TCAGTGCCTGGGTCCATTGG+TGG 0.564340 1:+89373679 MsG0180005267.01.T01:intron
AATGAAATCAAAGATGACTC+AGG 0.574790 1:+89373748 MsG0180005267.01.T01:CDS
CACAACTTTAAGAATGACAA+TGG 0.590296 1:+89374131 MsG0180005267.01.T01:CDS
GGACTTCTACAATCTAGAGT+TGG 0.602394 1:+89373920 MsG0180005267.01.T01:CDS
TAATGTTTGGTATCTTGGAG+TGG 0.602976 1:+89373702 MsG0180005267.01.T01:CDS
AATAATGGAGAGCGTGAAGG+TGG 0.607965 1:+89372474 None:intergenic
GGAAAACGGTCGTTAAGCGT+AGG 0.620167 1:+89373769 MsG0180005267.01.T01:CDS
AATAATAATGGAGAGCGTGA+AGG 0.623264 1:+89372471 None:intergenic
CCGGCGAACTTCAAAGACTA+CGG 0.628730 1:+89372523 MsG0180005267.01.T01:CDS
GCAAATGTGACTCAGTGCCT+GGG 0.634650 1:+89373668 MsG0180005267.01.T01:intron
ATGTAAGATTCCATTGAAAG+GGG 0.634905 1:-89373968 None:intergenic
ACATTACCACCAATGGACCC+AGG 0.650151 1:-89373685 None:intergenic
ATCGTGCTAGACTTAAATAG+AGG 0.664302 1:-89373897 None:intergenic
TCTATTTAAGTCTAGCACGA+TGG 0.669627 1:+89373899 MsG0180005267.01.T01:CDS
GACCAGCATGCCATGTACCG+CGG 0.687313 1:-89373875 None:intergenic
AAATTCGTCAAGCTTCACCG+CGG 0.805303 1:+89373858 MsG0180005267.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTAACATATAGAATT+TGG - Chr1:89372675-89372694 None:intergenic 10.0%
!!! AACTAATTTTAATAATATGT+TGG - Chr1:89373580-89373599 None:intergenic 10.0%
!!! ATTTTTTTCACTTTAATTTT+GGG + Chr1:89372696-89372715 MsG0180005267.01.T01:intron 10.0%
!! AAGGAAACAAAAAAAAAAAA+AGG - Chr1:89373312-89373331 None:intergenic 15.0%
!! TTAAAGATAACATTTATCTT+TGG + Chr1:89373186-89373205 MsG0180005267.01.T01:intron 15.0%
!!! ATTTTCTTATTTTTGTAAGT+TGG - Chr1:89372647-89372666 None:intergenic 15.0%
!!! GATTTTTTTCACTTTAATTT+TGG + Chr1:89372695-89372714 MsG0180005267.01.T01:intron 15.0%
!!! TAATTTTAATAATATGTTGG+AGG - Chr1:89373577-89373596 None:intergenic 15.0%
!! AATGAACTGATATGTTAAAA+AGG - Chr1:89373044-89373063 None:intergenic 20.0%
!! AGGAAATATATACATGATAA+TGG - Chr1:89373080-89373099 None:intergenic 20.0%
!! GAACTAGTAAAATTTATTAG+AGG + Chr1:89372902-89372921 MsG0180005267.01.T01:intron 20.0%
!! TGAAAAACTTACATTTGTAT+TGG - Chr1:89373946-89373965 None:intergenic 20.0%
!!! AAAATTAGTTTGTGTTTTGT+AGG + Chr1:89373590-89373609 MsG0180005267.01.T01:intron 20.0%
!!! AATACAAATGTAAGTTTTTC+AGG + Chr1:89373945-89373964 MsG0180005267.01.T01:CDS 20.0%
!!! CCAATAATATATAGTTTTGA+CGG - Chr1:89372985-89373004 None:intergenic 20.0%
!!! TGTCATTTTTTTTAATCGTT+TGG + Chr1:89374060-89374079 MsG0180005267.01.T01:intron 20.0%
!!! TTTTTTTTGTTTCCTTTTGA+TGG + Chr1:89373316-89373335 MsG0180005267.01.T01:intron 20.0%
! AAATGTGACGATTATTAGTA+TGG + Chr1:89373223-89373242 MsG0180005267.01.T01:intron 25.0%
! AATGTGACGATTATTAGTAT+GGG + Chr1:89373224-89373243 MsG0180005267.01.T01:intron 25.0%
! CATTATATTTCACACGAATA+AGG - Chr1:89373100-89373119 None:intergenic 25.0%
! CCGTCAAAACTATATATTAT+TGG + Chr1:89372982-89373001 MsG0180005267.01.T01:intron 25.0%
! TACTTTATAGCAAAATTGAG+AGG + Chr1:89373397-89373416 MsG0180005267.01.T01:intron 25.0%
! TATATACTACCCTATTATCT+TGG + Chr1:89373355-89373374 MsG0180005267.01.T01:intron 25.0%
!! ATCTAAGACATACTACAAAA+CGG - Chr1:89373021-89373040 None:intergenic 25.0%
!! TGATTCTAGAAAACTTTAGT+GGG - Chr1:89373642-89373661 None:intergenic 25.0%
!! TTTGCTATAAAGTAGTTTAG+TGG - Chr1:89373391-89373410 None:intergenic 25.0%
!!! TGCTTTTTTATTATGTGTAC+AGG + Chr1:89374091-89374110 MsG0180005267.01.T01:intron 25.0%
!!! TTTTCACTTTAATTTTGGGT+TGG + Chr1:89372700-89372719 MsG0180005267.01.T01:intron 25.0%
AAACGTTCGATCTTAATCAT+AGG + Chr1:89372764-89372783 MsG0180005267.01.T01:intron 30.0%
AATGAAATCAAAGATGACTC+AGG + Chr1:89373748-89373767 MsG0180005267.01.T01:CDS 30.0%
AGATGTAAGATTCCATTGAA+AGG - Chr1:89373973-89373992 None:intergenic 30.0%
AGTTTATCCATACCATCAAA+AGG - Chr1:89373331-89373350 None:intergenic 30.0%
ATGTAAGATTCCATTGAAAG+GGG - Chr1:89373971-89373990 None:intergenic 30.0%
CTATAAAGTAGTTTAGTGGT+GGG - Chr1:89373387-89373406 None:intergenic 30.0%
GATGTAAGATTCCATTGAAA+GGG - Chr1:89373972-89373991 None:intergenic 30.0%
GATTATTAGTATGGGACATA+GGG + Chr1:89373232-89373251 MsG0180005267.01.T01:intron 30.0%
TAATAATATGTTGGAGGACT+TGG - Chr1:89373571-89373590 None:intergenic 30.0%
TATCAGTTAAATTGAGCTGA+TGG - Chr1:89373499-89373518 None:intergenic 30.0%
TCATTTGAATCAACAACTGA+TGG - Chr1:89373733-89373752 None:intergenic 30.0%
TTGGTGAAATATGAAAACCT+GGG - Chr1:89372628-89372647 None:intergenic 30.0%
! CACAACTTTAAGAATGACAA+TGG + Chr1:89374131-89374150 MsG0180005267.01.T01:CDS 30.0%
! GTGATTCTAGAAAACTTTAG+TGG - Chr1:89373643-89373662 None:intergenic 30.0%
!! TTTGTTTCCTTTTGATGGTA+TGG + Chr1:89373321-89373340 MsG0180005267.01.T01:intron 30.0%
AGAGCATGTCAAATGAATTC+AGG - Chr1:89374004-89374023 None:intergenic 35.0%
AGTACCAAGTTTACACTGTT+TGG + Chr1:89373255-89373274 MsG0180005267.01.T01:intron 35.0%
ATACCAAACATTACCACCAA+TGG - Chr1:89373695-89373714 None:intergenic 35.0%
ATCGTGCTAGACTTAAATAG+AGG - Chr1:89373900-89373919 None:intergenic 35.0%
CGATTATTAGTATGGGACAT+AGG + Chr1:89373231-89373250 MsG0180005267.01.T01:intron 35.0%
GACCTGAAATCTTGAAAACT+TGG - Chr1:89373836-89373855 None:intergenic 35.0%
GCTATAAAGTAGTTTAGTGG+TGG - Chr1:89373388-89373407 None:intergenic 35.0%
GTACCAAGTTTACACTGTTT+GGG + Chr1:89373256-89373275 MsG0180005267.01.T01:intron 35.0%
TAATGTTTGGTATCTTGGAG+TGG + Chr1:89373702-89373721 MsG0180005267.01.T01:CDS 35.0%
TATAGCAAAATTGAGAGGCT+AGG + Chr1:89373402-89373421 MsG0180005267.01.T01:intron 35.0%
TCAAAGATGACTCAGGAAAA+CGG + Chr1:89373755-89373774 MsG0180005267.01.T01:CDS 35.0%
TCTATTTAAGTCTAGCACGA+TGG + Chr1:89373899-89373918 MsG0180005267.01.T01:CDS 35.0%
TGCAAAGTTTCTTTCTTCGA+TGG - Chr1:89373442-89373461 None:intergenic 35.0%
! GTTGGTGAAATATGAAAACC+TGG - Chr1:89372629-89372648 None:intergenic 35.0%
!! ATGCTCTTTAGAGTAAGACA+TGG + Chr1:89374017-89374036 MsG0180005267.01.T01:intron 35.0%
!! GTCCAAGTTTTCAAGATTTC+AGG + Chr1:89373831-89373850 MsG0180005267.01.T01:intron 35.0%
!! TGCTCTTTAGAGTAAGACAT+GGG + Chr1:89374018-89374037 MsG0180005267.01.T01:intron 35.0%
!! TTTGGGTTGGATTGATCATT+TGG + Chr1:89372713-89372732 MsG0180005267.01.T01:intron 35.0%
AAAACCTGGGAAATATCCCT+TGG - Chr1:89372615-89372634 None:intergenic 40.0%
AAACCTGGGAAATATCCCTT+GGG - Chr1:89372614-89372633 None:intergenic 40.0%
CAACCCAAACAGTGTAAACT+TGG - Chr1:89373262-89373281 None:intergenic 40.0%
GGGTGAATGCCAAGATAATA+GGG - Chr1:89373367-89373386 None:intergenic 40.0%
TGGGTGAATGCCAAGATAAT+AGG - Chr1:89373368-89373387 None:intergenic 40.0%
! GGACTTCTACAATCTAGAGT+TGG + Chr1:89373920-89373939 MsG0180005267.01.T01:CDS 40.0%
! GGTGGTAATGTTTGGTATCT+TGG + Chr1:89373697-89373716 MsG0180005267.01.T01:CDS 40.0%
!! AGATCAAGTTGAGCATCATG+GGG - Chr1:89372589-89372608 None:intergenic 40.0%
!! GAGATCAAGTTGAGCATCAT+GGG - Chr1:89372590-89372609 None:intergenic 40.0%
!! TGAGATCAAGTTGAGCATCA+TGG - Chr1:89372591-89372610 None:intergenic 40.0%
AAATTCGTCAAGCTTCACCG+CGG + Chr1:89373858-89373877 MsG0180005267.01.T01:CDS 45.0%
AGCAAAATTGAGAGGCTAGG+AGG + Chr1:89373405-89373424 MsG0180005267.01.T01:intron 45.0%
CGGTGAAGCTTGACGAATTT+GGG - Chr1:89373858-89373877 None:intergenic 45.0%
CGTGAAGGTGGTGACAATTA+AGG + Chr1:89372486-89372505 MsG0180005267.01.T01:CDS 45.0%
CTCAACTTGATCTCACCCAA+GGG + Chr1:89372596-89372615 MsG0180005267.01.T01:intron 45.0%
GAAAACTTGGACGTCCTCAA+CGG - Chr1:89373823-89373842 None:intergenic 45.0%
GAACTTCAAAGACTACGGTC+AGG + Chr1:89372528-89372547 MsG0180005267.01.T01:intron 45.0%
TAAGGCAATCGAAGCAACTC+CGG + Chr1:89372504-89372523 MsG0180005267.01.T01:CDS 45.0%
TGAGAGGCTAGGAGGAATAT+TGG + Chr1:89373413-89373432 MsG0180005267.01.T01:intron 45.0%
! GGTCCATTGGTGGTAATGTT+TGG + Chr1:89373689-89373708 MsG0180005267.01.T01:CDS 45.0%
!!! GTTTTTCAGGCCCCTTTCAA+TGG + Chr1:89373958-89373977 MsG0180005267.01.T01:CDS 45.0%
ACATTACCACCAATGGACCC+AGG - Chr1:89373688-89373707 None:intergenic 50.0%
CCGGCGAACTTCAAAGACTA+CGG + Chr1:89372523-89372542 MsG0180005267.01.T01:CDS 50.0%
GCAAATGTGACTCAGTGCCT+GGG + Chr1:89373668-89373687 MsG0180005267.01.T01:intron 50.0%
GCGGTGAAGCTTGACGAATT+TGG - Chr1:89373859-89373878 None:intergenic 50.0%
GCTCAACTTGATCTCACCCA+AGG + Chr1:89372595-89372614 MsG0180005267.01.T01:intron 50.0%
GGAAAACGGTCGTTAAGCGT+AGG + Chr1:89373769-89373788 MsG0180005267.01.T01:CDS 50.0%
TCACCCAAGGGATATTTCCC+AGG + Chr1:89372608-89372627 MsG0180005267.01.T01:intron 50.0%
TGCAAATGTGACTCAGTGCC+TGG + Chr1:89373667-89373686 MsG0180005267.01.T01:intron 50.0%
TTATGTTCCTCCCACCGTTG+AGG + Chr1:89373806-89373825 MsG0180005267.01.T01:intron 50.0%
!! CCGTAGTCTTTGAAGTTCGC+CGG - Chr1:89372526-89372545 None:intergenic 50.0%
AAACCCAGGCTGTCTGGTGA+AGG - Chr1:89372562-89372581 None:intergenic 55.0%
AACTTGGACGTCCTCAACGG+TGG - Chr1:89373820-89373839 None:intergenic 55.0%
ACGGTCGTTAAGCGTAGGTG+CGG + Chr1:89373774-89373793 MsG0180005267.01.T01:intron 55.0%
ACTTGGACGTCCTCAACGGT+GGG - Chr1:89373819-89373838 None:intergenic 55.0%
CATCATGGGGACCAAAACCC+AGG - Chr1:89372576-89372595 None:intergenic 55.0%
TCAAGCTTCACCGCGGTACA+TGG + Chr1:89373865-89373884 MsG0180005267.01.T01:CDS 55.0%
! TCACCAGACAGCCTGGGTTT+TGG + Chr1:89372562-89372581 MsG0180005267.01.T01:intron 55.0%
GACCAGCATGCCATGTACCG+CGG - Chr1:89373878-89373897 None:intergenic 60.0%
GACTCAGTGCCTGGGTCCAT+TGG + Chr1:89373676-89373695 MsG0180005267.01.T01:intron 60.0%
GAGCCTTCACCAGACAGCCT+GGG + Chr1:89372556-89372575 MsG0180005267.01.T01:intron 60.0%
GGACCAAAACCCAGGCTGTC+TGG - Chr1:89372568-89372587 None:intergenic 60.0%
GTCGTTAAGCGTAGGTGCGG+CGG + Chr1:89373777-89373796 MsG0180005267.01.T01:intron 60.0%
TCAGTGCCTGGGTCCATTGG+TGG + Chr1:89373679-89373698 MsG0180005267.01.T01:intron 60.0%
CACCGCGGTACATGGCATGC+TGG + Chr1:89373873-89373892 MsG0180005267.01.T01:CDS 65.0%
CGAGCCTTCACCAGACAGCC+TGG + Chr1:89372555-89372574 MsG0180005267.01.T01:intron 65.0%
TGGACGTCCTCAACGGTGGG+AGG - Chr1:89373816-89373835 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 89372478 89374178 89372478 ID=MsG0180005267.01;Name=MsG0180005267.01
Chr1 mRNA 89372478 89374178 89372478 ID=MsG0180005267.01.T01;Parent=MsG0180005267.01;Name=MsG0180005267.01.T01;_AED=0.50;_eAED=0.59;_QI=0|0|0|1|1|1|4|0|138
Chr1 exon 89372478 89372544 89372478 ID=MsG0180005267.01.T01:exon:17222;Parent=MsG0180005267.01.T01
Chr1 exon 89373683 89373790 89373683 ID=MsG0180005267.01.T01:exon:17223;Parent=MsG0180005267.01.T01
Chr1 exon 89373853 89374028 89373853 ID=MsG0180005267.01.T01:exon:17224;Parent=MsG0180005267.01.T01
Chr1 exon 89374113 89374178 89374113 ID=MsG0180005267.01.T01:exon:17225;Parent=MsG0180005267.01.T01
Chr1 CDS 89372478 89372544 89372478 ID=MsG0180005267.01.T01:cds;Parent=MsG0180005267.01.T01
Chr1 CDS 89373683 89373790 89373683 ID=MsG0180005267.01.T01:cds;Parent=MsG0180005267.01.T01
Chr1 CDS 89373853 89374028 89373853 ID=MsG0180005267.01.T01:cds;Parent=MsG0180005267.01.T01
Chr1 CDS 89374113 89374178 89374113 ID=MsG0180005267.01.T01:cds;Parent=MsG0180005267.01.T01
Gene Sequence

>MsG0180005267.01.T01

ATGGAGAGCGTGAAGGTGGTGACAATTAAGGCAATCGAAGCAACTCCGGCGAACTTCAAAGACTACGTGCCTGGGTCCATTGGTGGTAATGTTTGGTATCTTGGAGTGGCTAAGCCATCAGTTGTTGATTCAAATGAAATCAAAGATGACTCAGGAAAACGGTCGTTAAGCGTAGGTCCCAAATTCGTCAAGCTTCACCGCGGTACATGGCATGCTGGTCCTCTATTTAAGTCTAGCACGATGGACTTCTACAATCTAGAGTTGGCCAATACAAATGTAAGTTTTTCAGGCCCCTTTCAATGGAATCTTACATCTTACTTGCACCTGAATTCATTTGACATGCTCTTTAGAGAGAATGATATTACTACACACAACTTTAAGAATGACAATGGAGTGACTTTTACAATTGATGAGTAA

Protein sequence

>MsG0180005267.01.T01

MESVKVVTIKAIEATPANFKDYVPGSIGGNVWYLGVAKPSVVDSNEIKDDSGKRSLSVGPKFVKLHRGTWHAGPLFKSSTMDFYNLELANTNVSFSGPFQWNLTSYLHLNSFDMLFRENDITTHNFKNDNGVTFTIDE*