AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015115.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015115.01.T01 MTR_3g063030 87.302 126 16 0 17 142 36 161 3.87e-79 231
MsG0380015115.01.T01 MTR_3g062950 80.451 133 21 2 11 142 37 165 7.25e-72 213
MsG0380015115.01.T01 MTR_3g062940 70.588 119 35 0 24 142 78 196 1.33e-49 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015115.01.T01 AT5G58240 64.516 124 44 0 17 140 2 125 1.07e-56 173
MsG0380015115.01.T01 AT5G58240 65.289 121 42 0 20 140 37 157 2.18e-56 173
MsG0380015115.01.T01 AT5G58240 65.289 121 42 0 20 140 57 177 2.76e-56 174

Find 19 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGTTGTTTATTTACCTTAA+TGG 0.217298 3:-65414399 None:intergenic
ATGATGAGAACATTGATTTA+TGG 0.249450 3:+65415674 MsG0380015115.01.T01:CDS
CCTCTTGTGCCATTTCTTCA+AGG 0.271826 3:-65416191 None:intergenic
CAATGATGACATTTATGATT+TGG 0.306333 3:+65416001 MsG0380015115.01.T01:CDS
CTCCTCTACTCACTCCATTA+AGG 0.313058 3:+65414385 MsG0380015115.01.T01:CDS
TGGATTTAGAGAGGAAAGAT+AGG 0.366670 3:+65416166 MsG0380015115.01.T01:CDS
AGATAGGAGCCTTGAAGAAA+TGG 0.398545 3:+65416182 MsG0380015115.01.T01:CDS
ACACGCTTCACTTCGCGCTT+CGG 0.426783 3:-65415637 None:intergenic
ATGTAAGTGATGATGCATTA+TGG 0.441950 3:-65415735 None:intergenic
AACCAGGATGGACCTCAAGC+TGG 0.511868 3:+65415918 MsG0380015115.01.T01:intron
TTGTTCTTGTATACACATCA+AGG 0.538035 3:+65414351 MsG0380015115.01.T01:CDS
TTCATATCCATATACTTCCT+CGG 0.540594 3:+65415958 MsG0380015115.01.T01:CDS
CCTTGAAGAAATGGCACAAG+AGG 0.544026 3:+65416191 MsG0380015115.01.T01:CDS
TGGCGCATAGCACATAAACT+TGG 0.555803 3:+65415694 MsG0380015115.01.T01:CDS
TACCTTAATGGAGTGAGTAG+AGG 0.565031 3:-65414387 None:intergenic
GGAACTGATTGTCCAGCTTG+AGG 0.613109 3:-65415930 None:intergenic
GTTGAAAAGTAAGCTAGCTG+TGG 0.621405 3:+65416146 MsG0380015115.01.T01:CDS
AGCTAGCTGTGGATTTAGAG+AGG 0.624532 3:+65416157 MsG0380015115.01.T01:CDS
AGTATATGGATATGAACATG+AGG 0.648556 3:-65415951 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTCCTAAAATATTT+AGG - Chr3:65414910-65414929 None:intergenic 10.0%
!! AAAGAAAAAAATTATTGTGA+GGG + Chr3:65415240-65415259 MsG0380015115.01.T01:intron 15.0%
!! CTAAAAATCAAGTAAAAATT+TGG + Chr3:65416074-65416093 MsG0380015115.01.T01:intron 15.0%
!!! AAAAAATGCCTAAATATTTT+AGG + Chr3:65414899-65414918 MsG0380015115.01.T01:intron 15.0%
!!! AGATTAATGTTACTTTTATT+GGG + Chr3:65414469-65414488 MsG0380015115.01.T01:intron 15.0%
!!! CATTTGTGTTTTTAATAATT+TGG + Chr3:65416034-65416053 MsG0380015115.01.T01:intron 15.0%
!!! TATTGTTATTTGAGTATATA+AGG + Chr3:65415787-65415806 MsG0380015115.01.T01:intron 15.0%
!! AGTAACATTAATCTCATATA+TGG - Chr3:65414463-65414482 None:intergenic 20.0%
!! GAAAGAAAAAAATTATTGTG+AGG + Chr3:65415239-65415258 MsG0380015115.01.T01:intron 20.0%
!! GTTAATCTATCGTTATAATA+TGG + Chr3:65414984-65415003 MsG0380015115.01.T01:intron 20.0%
!! TAGTTGTTTATTTACCTTAA+TGG - Chr3:65414402-65414421 None:intergenic 20.0%
!! TTATTAACATCACACTAATA+AGG - Chr3:65414719-65414738 None:intergenic 20.0%
!! TTTCTTTCTTTATAAACTAG+AGG - Chr3:65415227-65415246 None:intergenic 20.0%
!!! AAGTTAACATTTGATGATTA+TGG - Chr3:65414426-65414445 None:intergenic 20.0%
!!! ATTAATGTTACTTTTATTGG+GGG + Chr3:65414471-65414490 MsG0380015115.01.T01:intron 20.0%
!!! ATTTTGTTAGAAATTTGTCT+GGG + Chr3:65415406-65415425 MsG0380015115.01.T01:intron 20.0%
!!! CTTTTAATGTAGAGTATATA+AGG + Chr3:65415815-65415834 MsG0380015115.01.T01:intron 20.0%
!!! GAGATTAATGTTACTTTTAT+TGG + Chr3:65414468-65414487 MsG0380015115.01.T01:intron 20.0%
!!! GATTAATGTTACTTTTATTG+GGG + Chr3:65414470-65414489 MsG0380015115.01.T01:intron 20.0%
! AAAAGATAGCATCTCAATAT+AGG + Chr3:65415312-65415331 MsG0380015115.01.T01:intron 25.0%
! AATGTAAACATGAATCTACT+TGG + Chr3:65415555-65415574 MsG0380015115.01.T01:intron 25.0%
! ATGGAACATAGTAATACATA+GGG + Chr3:65414953-65414972 MsG0380015115.01.T01:intron 25.0%
! ATTCTTCAAATTGTCTTACT+TGG - Chr3:65415768-65415787 None:intergenic 25.0%
! CAATGATGACATTTATGATT+TGG + Chr3:65416001-65416020 MsG0380015115.01.T01:CDS 25.0%
! GAGATTTAGATTTAGAGATA+TGG + Chr3:65414934-65414953 MsG0380015115.01.T01:intron 25.0%
! TATGGAACATAGTAATACAT+AGG + Chr3:65414952-65414971 MsG0380015115.01.T01:intron 25.0%
! TCAAAACCTTAATATCATCA+CGG + Chr3:65415503-65415522 MsG0380015115.01.T01:intron 25.0%
!! CATTTTGTTAGAAATTTGTC+TGG + Chr3:65415405-65415424 MsG0380015115.01.T01:intron 25.0%
!!! ATGATGAGAACATTGATTTA+TGG + Chr3:65415674-65415693 MsG0380015115.01.T01:CDS 25.0%
ACAAAACAAGTGCTTAAAAG+TGG - Chr3:65415196-65415215 None:intergenic 30.0%
ACAACAACATTTGATATGTC+CGG + Chr3:65415049-65415068 MsG0380015115.01.T01:intron 30.0%
AGTAATACATAGGGTAGTAA+TGG + Chr3:65414962-65414981 MsG0380015115.01.T01:intron 30.0%
AGTATATGGATATGAACATG+AGG - Chr3:65415954-65415973 None:intergenic 30.0%
ATATGAGCAATAGATCAAAG+AGG - Chr3:65414633-65414652 None:intergenic 30.0%
ATGACCTGCAAAAGATAAAA+AGG - Chr3:65414694-65414713 None:intergenic 30.0%
ATTGTATAGCCATATAGCAT+TGG - Chr3:65415869-65415888 None:intergenic 30.0%
GCAGATAAATGAAAAAGAGA+AGG + Chr3:65416122-65416141 MsG0380015115.01.T01:intron 30.0%
GCTGTATTTGTAATCACATA+AGG + Chr3:65415468-65415487 MsG0380015115.01.T01:intron 30.0%
TTCATATCCATATACTTCCT+CGG + Chr3:65415958-65415977 MsG0380015115.01.T01:CDS 30.0%
TTGTATGAAACACTGAACTA+TGG - Chr3:65414549-65414568 None:intergenic 30.0%
TTGTTCTTGTATACACATCA+AGG + Chr3:65414351-65414370 MsG0380015115.01.T01:CDS 30.0%
TTTGTAATCACATAAGGCTA+TGG + Chr3:65415474-65415493 MsG0380015115.01.T01:intron 30.0%
! AATTGACACACTCTTTTGTT+AGG + Chr3:65415266-65415285 MsG0380015115.01.T01:intron 30.0%
! ATGTAAGTGATGATGCATTA+TGG - Chr3:65415738-65415757 None:intergenic 30.0%
! CTTTCCTTTTTATCTTTTGC+AGG + Chr3:65414687-65414706 MsG0380015115.01.T01:intron 30.0%
! GCTGTAGAATACTATGATTT+TGG + Chr3:65414508-65414527 MsG0380015115.01.T01:intron 30.0%
!! ACATTTATGATTTGGTAGGA+TGG + Chr3:65416009-65416028 MsG0380015115.01.T01:intron 30.0%
!! GATGACATTTATGATTTGGT+AGG + Chr3:65416005-65416024 MsG0380015115.01.T01:intron 30.0%
!!! AGATGCTATCTTTTGAGTTT+GGG - Chr3:65415306-65415325 None:intergenic 30.0%
!!! TTTTAAGCACTTGTTTTGTG+AGG + Chr3:65415197-65415216 MsG0380015115.01.T01:intron 30.0%
ACACAACAAGTGCTTAAAAG+AGG - Chr3:65415099-65415118 None:intergenic 35.0%
ACAGTAATCACTAACCAATG+TGG - Chr3:65415382-65415401 None:intergenic 35.0%
AGCATGTTCAAATCAACAAC+CGG - Chr3:65415071-65415090 None:intergenic 35.0%
ATGGTAAATGTATGTCTCGT+AGG + Chr3:65415003-65415022 MsG0380015115.01.T01:intron 35.0%
CAAATCAATCTTTCTGAACC+AGG + Chr3:65415902-65415921 MsG0380015115.01.T01:intron 35.0%
CATATACTTCCTCGGAAAAA+CGG + Chr3:65415966-65415985 MsG0380015115.01.T01:CDS 35.0%
CTGGTTCAGAAAGATTGATT+TGG - Chr3:65415904-65415923 None:intergenic 35.0%
GACAAAGGCAAAAGAAAGAT+CGG - Chr3:65414572-65414591 None:intergenic 35.0%
GCTTCATCATATAACCACAT+TGG + Chr3:65415365-65415384 MsG0380015115.01.T01:intron 35.0%
TATGAAACACTGAACTATGG+TGG - Chr3:65414546-65414565 None:intergenic 35.0%
TGGATTTAGAGAGGAAAGAT+AGG + Chr3:65416166-65416185 MsG0380015115.01.T01:CDS 35.0%
TTGTAGCCGTGATGATATTA+AGG - Chr3:65415512-65415531 None:intergenic 35.0%
! ATTGGGGGTGTAAATAAAGA+TGG + Chr3:65414486-65414505 MsG0380015115.01.T01:intron 35.0%
!!! GAGATGCTATCTTTTGAGTT+TGG - Chr3:65415307-65415326 None:intergenic 35.0%
!!! TTTTAAGCACTTGTTGTGTG+AGG + Chr3:65415100-65415119 MsG0380015115.01.T01:intron 35.0%
AGATAGGAGCCTTGAAGAAA+TGG + Chr3:65416182-65416201 MsG0380015115.01.T01:CDS 40.0%
AGCAATAGATCAAAGAGGTG+AGG - Chr3:65414628-65414647 None:intergenic 40.0%
GCAATAGATCAAAGAGGTGA+GGG - Chr3:65414627-65414646 None:intergenic 40.0%
GCTTAAAAGTGGAGAATAGC+AGG - Chr3:65415185-65415204 None:intergenic 40.0%
GTTGAAAAGTAAGCTAGCTG+TGG + Chr3:65416146-65416165 MsG0380015115.01.T01:CDS 40.0%
TACCTTAATGGAGTGAGTAG+AGG - Chr3:65414390-65414409 None:intergenic 40.0%
TCAATCTTTCTGAACCAGGA+TGG + Chr3:65415906-65415925 MsG0380015115.01.T01:intron 40.0%
TGCCTAAGACCAATGCTATA+TGG + Chr3:65415857-65415876 MsG0380015115.01.T01:intron 40.0%
!! AGCCATATAGCATTGGTCTT+AGG - Chr3:65415862-65415881 None:intergenic 40.0%
!! CGTTTTTCCGAGGAAGTATA+TGG - Chr3:65415968-65415987 None:intergenic 40.0%
AGCTAGCTGTGGATTTAGAG+AGG + Chr3:65416157-65416176 MsG0380015115.01.T01:CDS 45.0%
CCTTGAAGAAATGGCACAAG+AGG + Chr3:65416191-65416210 MsG0380015115.01.T01:CDS 45.0%
CTCCTCTACTCACTCCATTA+AGG + Chr3:65414385-65414404 MsG0380015115.01.T01:CDS 45.0%
TGGCGCATAGCACATAAACT+TGG + Chr3:65415694-65415713 MsG0380015115.01.T01:CDS 45.0%
!! CCTCTTGTGCCATTTCTTCA+AGG - Chr3:65416194-65416213 None:intergenic 45.0%
!! TCGAAATCACCGTTTTTCCG+AGG - Chr3:65415978-65415997 None:intergenic 45.0%
AGCAAGATACCAGTGACAGC+AGG - Chr3:65414604-65414623 None:intergenic 50.0%
GGAACTGATTGTCCAGCTTG+AGG - Chr3:65415933-65415952 None:intergenic 50.0%
AACCAGGATGGACCTCAAGC+TGG + Chr3:65415918-65415937 MsG0380015115.01.T01:intron 55.0%
ACACGCTTCACTTCGCGCTT+CGG - Chr3:65415640-65415659 None:intergenic 55.0%
! AGCAGGACGCAGGTTGACAA+AGG - Chr3:65414587-65414606 None:intergenic 55.0%
AACCTGCGTCCTGCTGTCAC+TGG + Chr3:65414592-65414611 MsG0380015115.01.T01:intron 60.0%
GTCCAGCTTGAGGTCCATCC+TGG - Chr3:65415923-65415942 None:intergenic 60.0%
TACCAGTGACAGCAGGACGC+AGG - Chr3:65414597-65414616 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 65414344 65416242 65414344 ID=MsG0380015115.01;Name=MsG0380015115.01
Chr3 mRNA 65414344 65416242 65414344 ID=MsG0380015115.01.T01;Parent=MsG0380015115.01;Name=MsG0380015115.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|0|0|4|0|142
Chr3 exon 65414344 65414406 65414344 ID=MsG0380015115.01.T01:exon:22756;Parent=MsG0380015115.01.T01
Chr3 exon 65415619 65415768 65415619 ID=MsG0380015115.01.T01:exon:22757;Parent=MsG0380015115.01.T01
Chr3 exon 65415924 65416022 65415924 ID=MsG0380015115.01.T01:exon:22758;Parent=MsG0380015115.01.T01
Chr3 exon 65416126 65416242 65416126 ID=MsG0380015115.01.T01:exon:22759;Parent=MsG0380015115.01.T01
Chr3 CDS 65414344 65414406 65414344 ID=MsG0380015115.01.T01:cds;Parent=MsG0380015115.01.T01
Chr3 CDS 65415619 65415768 65415619 ID=MsG0380015115.01.T01:cds;Parent=MsG0380015115.01.T01
Chr3 CDS 65415924 65416022 65415924 ID=MsG0380015115.01.T01:cds;Parent=MsG0380015115.01.T01
Chr3 CDS 65416126 65416242 65416126 ID=MsG0380015115.01.T01:cds;Parent=MsG0380015115.01.T01
Gene Sequence

>MsG0380015115.01.T01

CTACTGATTGTTCTTGTATACACATCAAGGAGCATTATTATCTCCTCTACTCACTCCATTAAGCTACATGTTCTAATCTGTCCGAAGCGCGAAGTGAAGCGTGTTGCTGATCTTACTGATGATGAGAACATTGATTTATGGCGCATAGCACATAAACTTGGTAGACAACTTGAGAGCTACCATAATGCATCATCACTTACATTTTGTATCCAAGATGGACCTCAAGCTGGACAATCAGTTCCTCATGTTCATATCCATATACTTCCTCGGAAAAACGGTGATTTCGAAAACAATGATGACATTTATGATTTGATAAATGAAAAAGAGAAGGAGTTGAAAAGTAAGCTAGCTGTGGATTTAGAGAGGAAAGATAGGAGCCTTGAAGAAATGGCACAAGAGGCTGATGAATACAGAAAATTTGTCCTTTGA

Protein sequence

>MsG0380015115.01.T01

LLIVLVYTSRSIIISSTHSIKLHVLICPKREVKRVADLTDDENIDLWRIAHKLGRQLESYHNASSLTFCIQDGPQAGQSVPHVHIHILPRKNGDFENNDDIYDLINEKEKELKSKLAVDLERKDRSLEEMAQEADEYRKFVL*