AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013940.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013940.01.T03 MTR_2g076050 92.593 27 2 0 1 27 1 27 1.34e-12 55.1
MsG0380013940.01.T02 MTR_2g076050 69.231 52 10 1 2 47 9 60 1.17e-17 68.6
MsG0380013940.01.T01 MTR_0002s0700 97.590 83 2 0 1 83 1 83 7.54e-55 164
MsG0380013940.01.T01 MTR_4g050850 98.765 81 1 0 1 81 1 81 1.29e-53 163
MsG0380013940.01.T01 MTR_5g055290 98.684 76 1 0 1 76 1 76 1.50e-49 151
MsG0380013940.01.T01 MTR_6g007780 94.737 38 2 0 24 61 75 112 5.05e-20 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013940.01.T03 ATCG00570 97.436 39 1 0 1 39 1 39 4.57e-22 78.6
MsG0380013940.01.T01 ATCG00580 97.590 83 2 0 1 83 1 83 6.84e-55 164

Find 28 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 3:-48471286 MsG0380013940.01.T01:CDS
TCAAAACGGCCAGTTATTAA+TGG 0.307608 3:+48471217 None:intergenic
GTAGCTCTATAATTCGGATT+AGG 0.338134 3:+48471024 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 3:+48471320 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 3:-48471369 MsG0380013940.01.T01:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 3:-48471421 None:intergenic
ATACCCTCCCTATTCATTGC+GGG 0.391873 3:-48471328 MsG0380013940.01.T01:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 3:+48471321 None:intergenic
CTTTCTGTAAAATACTCGTT+TGG 0.412027 3:+48471253 None:intergenic
CAAGGAATTCCATTAATAAC+TGG 0.412082 3:-48471226 MsG0380013940.01.T01:CDS
TGTGTCGTAGCTCTATAATT+CGG 0.415726 3:+48471018 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 3:+48471363 None:intergenic
TTATTAATGGAATTCCTTGT+CGG 0.434610 3:+48471230 None:intergenic
AATGGGATAGGTCCTATCTA+TGG 0.452739 3:+48471141 None:intergenic
TAGGTCCTATCTATGGTCAT+TGG 0.484830 3:+48471148 None:intergenic
AGGCTCCAATGACCATAGAT+AGG 0.506879 3:-48471153 None:intergenic
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 3:-48471412 None:intergenic
ATTCGGATTAGGTTTATCGT+TGG 0.512045 3:+48471035 None:intergenic
GTACGATGGTTGGCTGTTCA+CGG 0.514822 3:-48471112 MsG0380013940.01.T03:CDS
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 3:-48471307 MsG0380013940.01.T01:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 3:-48471308 MsG0380013940.01.T01:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 3:-48471324 MsG0380013940.01.T01:CDS
GTAAAATACTCGTTTGGACG+AGG 0.550593 3:+48471259 None:intergenic
GTTGTTCCAAAGAATCAAAA+CGG 0.551629 3:+48471203 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 3:-48471329 MsG0380013940.01.T01:CDS
GTTGGCTGTTCACGGACTAG+CGG 0.657866 3:-48471104 MsG0380013940.01.T02:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 3:+48471325 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 3:+48471324 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTCAACATTTTGTTCATT+TGG + Chr3:48471407-48471426 None:intergenic 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG + Chr3:48471406-48471425 None:intergenic 20.0%
!!! TGAATTTAGTAGATCTTTTT+AGG - Chr3:48471216-48471235 MsG0380013940.01.T01:CDS 20.0%
! TATTGATCCCAAAAAAAAGA+CGG + Chr3:48471320-48471339 None:intergenic 25.0%
! TTATTAATGGAATTCCTTGT+CGG + Chr3:48471165-48471184 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG - Chr3:48471219-48471238 MsG0380013940.01.T01:CDS 25.0%
AACCATCGTACTGTAAAAAT+GGG + Chr3:48471271-48471290 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG - Chr3:48471166-48471185 MsG0380013940.01.T05:CDS 30.0%
CTTTCTGTAAAATACTCGTT+TGG + Chr3:48471142-48471161 None:intergenic 30.0%
GTTGTTCCAAAGAATCAAAA+CGG + Chr3:48471192-48471211 None:intergenic 30.0%
TTATTACCAGTATTCGATAC+TGG - Chr3:48471023-48471042 MsG0380013940.01.T02:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG + Chr3:48471032-48471051 None:intergenic 30.0%
CAACCATCGTACTGTAAAAA+TGG + Chr3:48471272-48471291 None:intergenic 35.0%
TCAAAACGGCCAGTTATTAA+TGG + Chr3:48471178-48471197 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG + Chr3:48471266-48471285 None:intergenic 35.0%
! ATTCGGATTAGGTTTATCGT+TGG + Chr3:48471360-48471379 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG - Chr3:48471270-48471289 MsG0380013940.01.T01:CDS 35.0%
! GGTTTAGCTTACGATGTATT+TGG - Chr3:48471106-48471125 MsG0380013940.01.T02:CDS 35.0%
! GTAGCTCTATAATTCGGATT+AGG + Chr3:48471371-48471390 None:intergenic 35.0%
! TATTTTACAGAAAGCCGACA+AGG - Chr3:48471148-48471167 MsG0380013940.01.T03:CDS 35.0%
! TGTGTCGTAGCTCTATAATT+CGG + Chr3:48471377-48471396 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG - Chr3:48471266-48471285 MsG0380013940.01.T01:CDS 35.0%
AATGGGATAGGTCCTATCTA+TGG + Chr3:48471254-48471273 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG + Chr3:48471075-48471094 None:intergenic 40.0%
GATCCCAAAAAAAAGACGGT+GGG + Chr3:48471316-48471335 None:intergenic 40.0%
GTAAAATACTCGTTTGGACG+AGG + Chr3:48471136-48471155 None:intergenic 40.0%
TAACCAACCCGCAATGAATA+GGG + Chr3:48471074-48471093 None:intergenic 40.0%
TAGGTCCTATCTATGGTCAT+TGG + Chr3:48471247-48471266 None:intergenic 40.0%
TATACCCTCCCTATTCATTG+CGG - Chr3:48471063-48471082 MsG0380013940.01.T02:CDS 40.0%
TGATCCCAAAAAAAAGACGG+TGG + Chr3:48471317-48471336 None:intergenic 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG - Chr3:48471183-48471202 MsG0380013940.01.T01:CDS 40.0%
!!! GTACCCACCGTCTTTTTTTT+GGG - Chr3:48471310-48471329 MsG0380013940.01.T01:CDS 40.0%
AGGCTCCAATGACCATAGAT+AGG - Chr3:48471239-48471258 MsG0380013940.01.T01:CDS 45.0%
ATACCCTCCCTATTCATTGC+GGG - Chr3:48471064-48471083 MsG0380013940.01.T02:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG - Chr3:48471085-48471104 MsG0380013940.01.T02:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG - Chr3:48471084-48471103 MsG0380013940.01.T02:CDS 45.0%
!!! GGTACCCACCGTCTTTTTTT+TGG - Chr3:48471309-48471328 MsG0380013940.01.T01:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG + Chr3:48471070-48471089 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG - Chr3:48471280-48471299 MsG0380013940.01.T01:CDS 50.0%
CCAACCCGCAATGAATAGGG+AGG + Chr3:48471071-48471090 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG - Chr3:48471068-48471087 MsG0380013940.01.T02:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG - Chr3:48471288-48471307 MsG0380013940.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48470986 48471428 48470986 ID=MsG0380013940.01;Name=MsG0380013940.01
Chr3 mRNA 48471177 48471428 48471177 ID=MsG0380013940.01.T01;Parent=MsG0380013940.01;Name=MsG0380013940.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|83
Chr3 exon 48471177 48471428 48471177 ID=MsG0380013940.01.T01:exon:4183;Parent=MsG0380013940.01.T01
Chr3 CDS 48471177 48471428 48471177 ID=MsG0380013940.01.T01:cds;Parent=MsG0380013940.01.T01
Chr3 mRNA 48470986 48471129 48470986 ID=MsG0380013940.01.T02;Parent=MsG0380013940.01;Name=MsG0380013940.01.T02;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|0|1|0|47
Chr3 exon 48470986 48471129 48470986 ID=MsG0380013940.01.T02:exon:4184;Parent=MsG0380013940.01.T02
Chr3 CDS 48470986 48471129 48470986 ID=MsG0380013940.01.T02:cds;Parent=MsG0380013940.01.T02
Chr3 mRNA 48471048 48471167 48471048 ID=MsG0380013940.01.T03;Parent=MsG0380013940.01;Name=MsG0380013940.01.T03;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|39
Chr3 exon 48471048 48471167 48471048 ID=MsG0380013940.01.T03:exon:4185;Parent=MsG0380013940.01.T03
Chr3 CDS 48471048 48471167 48471048 ID=MsG0380013940.01.T03:cds;Parent=MsG0380013940.01.T03
Chr3 mRNA 48471233 48471295 48471233 ID=MsG0380013940.01.T04;Parent=MsG0380013940.01;Name=MsG0380013940.01.T04;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|20
Chr3 exon 48471233 48471295 48471233 ID=MsG0380013940.01.T04:exon:4186;Parent=MsG0380013940.01.T04
Chr3 CDS 48471233 48471295 48471233 ID=MsG0380013940.01.T04:cds;Parent=MsG0380013940.01.T04
Chr3 mRNA 48471164 48471199 48471164 ID=MsG0380013940.01.T05;Parent=MsG0380013940.01;Name=MsG0380013940.01.T05;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|11
Chr3 exon 48471164 48471199 48471164 ID=MsG0380013940.01.T05:exon:4187;Parent=MsG0380013940.01.T05
Chr3 CDS 48471164 48471199 48471164 ID=MsG0380013940.01.T05:cds;Parent=MsG0380013940.01.T05
Chr3 mRNA 48470986 48471006 48470986 ID=MsG0380013940.01.T06;Parent=MsG0380013940.01;Name=MsG0380013940.01.T06;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|6
Chr3 exon 48470986 48471006 48470986 ID=MsG0380013940.01.T06:exon:4188;Parent=MsG0380013940.01.T06
Chr3 CDS 48470986 48471006 48470986 ID=MsG0380013940.01.T06:cds;Parent=MsG0380013940.01.T06
Chr3 mRNA 48471048 48471068 48471048 ID=MsG0380013940.01.T07;Parent=MsG0380013940.01;Name=MsG0380013940.01.T07;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|6
Chr3 exon 48471048 48471068 48471048 ID=MsG0380013940.01.T07:exon:4189;Parent=MsG0380013940.01.T07
Chr3 CDS 48471048 48471068 48471048 ID=MsG0380013940.01.T07:cds;Parent=MsG0380013940.01.T07
Gene Sequence

>MsG0380013940.01.T03

ATGACCATAGATAGGACCTATCCCATTTTTACAGTACGATGGTTGGCTGTTCACGGACTAGCGGTACCCACCGTCTTTTTTTTGGGATCAATATCAGCAATGCAGTTCATCCAACGATAA

>MsG0380013940.01.T05

ATGAATTTAGTAGATCTTTTTAGGAGGCTCCAATGA

>MsG0380013940.01.T07

ATGCAGTTCATCCAACGATAA

>MsG0380013940.01.T02

ATGGTTGGCTGTTCACGGACTAGCGGTACCCACCGTCTTTTTTTTGGGATCAATATCAGCAATGCAGTTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATTGA

>MsG0380013940.01.T06

ATGAACAAAATGTTGAATTGA

>MsG0380013940.01.T04

ATGTATTTGGAAGTCCTCGTCCAAACGAGTATTTTACAGAAAGCCGACAAGGAATTCCATTAA

>MsG0380013940.01.T01

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGAGTATTTTACAGAAAGCCGACAAGGAATTCCATTAATAACTGGCCGTTTTGATTCTTTGGAACAACTCGATGAATTTAGTAGATCTTTTTAG

Protein sequence

>MsG0380013940.01.T03

MTIDRTYPIFTVRWLAVHGLAVPTVFFLGSISAMQFIQR*

>MsG0380013940.01.T05

MNLVDLFRRLQ*

>MsG0380013940.01.T07

MQFIQR*

>MsG0380013940.01.T02

MVGCSRTSGTHRLFFGINISNAVHPTINLIRIIELRHNQTQMNKMLN*

>MsG0380013940.01.T06

MNKMLN*

>MsG0380013940.01.T04

MYLEVLVQTSILQKADKEFH*

>MsG0380013940.01.T01

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQGIPLITGRFDSLEQLDEFSRSF*