AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013941.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013941.01.T01 MTR_7g021800 99.367 158 1 0 1 158 1 158 3.47e-117 328
MsG0380013941.01.T01 MTR_4g035040 99.367 158 1 0 1 158 1 158 3.47e-117 328
MsG0380013941.01.T01 MTR_0002s0630 87.898 157 19 0 2 158 162 318 1.60e-102 296
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013941.01.T01 ATCG00420 89.241 158 17 0 1 158 1 158 2.45e-106 300

Find 26 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAATAGTTCATAGATCTT+TGG 0.225646 3:-48475205 MsG0380013941.01.T01:CDS
GGCCATCCTATCCAACTTTC+GGG 0.254679 3:+48474841 None:intergenic
GGAATAGTTCATAGATCTTT+GGG 0.328701 3:-48475204 MsG0380013941.01.T01:CDS
AGGCCATCCTATCCAACTTT+CGG 0.340000 3:+48474840 None:intergenic
TTCCAAACCCAGAAAATAGA+AGG 0.366444 3:+48474949 None:intergenic
TGCAGGGTCATTTGTCTGCT+TGG 0.409987 3:-48475239 MsG0380013941.01.T01:CDS
AGGGAATCTTATGATATGTT+AGG 0.415137 3:-48474907 MsG0380013941.01.T01:CDS
TTTGTAAAGTCTCTATTCCT+TGG 0.436961 3:+48475172 None:intergenic
TGCTACATCATAGGCACATT+GGG 0.447727 3:+48475083 None:intergenic
TCCTCCTGGTGCTACATCAT+AGG 0.453068 3:+48475074 None:intergenic
TACAAATAAAGCCCGAGGAT+TGG 0.464528 3:-48475155 MsG0380013941.01.T01:CDS
CAGAAAATAGAAGGAATTCT+CGG 0.478109 3:+48474958 None:intergenic
AGCAATCGAATGCCAATCCT+CGG 0.513209 3:+48475143 None:intergenic
TGCCCGAAAGTTGGATAGGA+TGG 0.516198 3:-48474843 MsG0380013941.01.T01:CDS
GTGCTACATCATAGGCACAT+TGG 0.546406 3:+48475082 None:intergenic
TTAATGCCCGAAAGTTGGAT+AGG 0.556642 3:-48474847 MsG0380013941.01.T01:CDS
ACACTAGCTAATAGTCCTCC+TGG 0.563367 3:+48475060 None:intergenic
GCAATCGAATGCCAATCCTC+GGG 0.577805 3:+48475144 None:intergenic
TGTGCCTATGATGTAGCACC+AGG 0.591745 3:-48475078 MsG0380013941.01.T01:CDS
GCGATATAATCTTTACGCAA+AGG 0.602633 3:+48474820 None:intergenic
GCATAAAAGTATTTGCCCCA+AGG 0.613297 3:-48474987 MsG0380013941.01.T01:CDS
TAGTTTAGATCAACCCGAAG+AGG 0.615869 3:-48475013 MsG0380013941.01.T01:CDS
ATTAAAATACGTTTCAGACG+CGG 0.619896 3:+48474865 None:intergenic
GACTTTACAAATAAAGCCCG+AGG 0.624427 3:-48475160 MsG0380013941.01.T01:CDS
TCTGCTTGGCTAGTCAAACA+CGG 0.639180 3:-48475225 MsG0380013941.01.T01:CDS
GCCTATGATGTAGCACCAGG+AGG 0.695694 3:-48475075 MsG0380013941.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCCTGTATTTCATAAAAAT+TGG + Chr3:48475234-48475253 None:intergenic 20.0%
!! TCCTGTATTTCATAAAAATT+GGG + Chr3:48475233-48475252 None:intergenic 20.0%
!!! GCTGTTATTTTATATGTATA+TGG - Chr3:48474903-48474922 MsG0380013941.01.T01:CDS 20.0%
! CCTGTATTTCATAAAAATTG+GGG + Chr3:48475232-48475251 None:intergenic 25.0%
! CTGTATTTCATAAAAATTGG+GGG + Chr3:48475231-48475250 None:intergenic 25.0%
AGGGAATCTTATGATATGTT+AGG - Chr3:48475119-48475138 MsG0380013941.01.T01:CDS 30.0%
ATTAAAATACGTTTCAGACG+CGG + Chr3:48475164-48475183 None:intergenic 30.0%
CAGAAAATAGAAGGAATTCT+CGG + Chr3:48475071-48475090 None:intergenic 30.0%
GGAATAGTTCATAGATCTTT+GGG - Chr3:48474822-48474841 MsG0380013941.01.T01:CDS 30.0%
TTTGTAAAGTCTCTATTCCT+TGG + Chr3:48474857-48474876 None:intergenic 30.0%
! CCCCAATTTTTATGAAATAC+AGG - Chr3:48475229-48475248 MsG0380013941.01.T01:CDS 30.0%
! GAGAATTCCTTCTATTTTCT+GGG - Chr3:48475070-48475089 MsG0380013941.01.T01:CDS 30.0%
! TACTTTTATGCATACCTCTT+CGG + Chr3:48475030-48475049 None:intergenic 30.0%
!!! AAAGTGCTGATTTTCAAGAA+AGG - Chr3:48475099-48475118 MsG0380013941.01.T01:CDS 30.0%
!!! AAGTGCTGATTTTCAAGAAA+GGG - Chr3:48475100-48475119 MsG0380013941.01.T01:CDS 30.0%
CGGAATAGTTCATAGATCTT+TGG - Chr3:48474821-48474840 MsG0380013941.01.T01:CDS 35.0%
GCGATATAATCTTTACGCAA+AGG + Chr3:48475209-48475228 None:intergenic 35.0%
TTCCAAACCCAGAAAATAGA+AGG + Chr3:48475080-48475099 None:intergenic 35.0%
! ACTTTTATGCATACCTCTTC+GGG + Chr3:48475029-48475048 None:intergenic 35.0%
! CGAGAATTCCTTCTATTTTC+TGG - Chr3:48475069-48475088 MsG0380013941.01.T01:CDS 35.0%
! TTCCTTCTATTTTCTGGGTT+TGG - Chr3:48475075-48475094 MsG0380013941.01.T01:CDS 35.0%
!!! GTATTTTAATGCCCGAAAGT+TGG - Chr3:48475174-48475193 MsG0380013941.01.T01:CDS 35.0%
!!! TCTTTGGGTTTTGATTACCA+AGG - Chr3:48474837-48474856 MsG0380013941.01.T01:CDS 35.0%
GACTTTACAAATAAAGCCCG+AGG - Chr3:48474866-48474885 MsG0380013941.01.T01:CDS 40.0%
TACAAATAAAGCCCGAGGAT+TGG - Chr3:48474871-48474890 MsG0380013941.01.T01:CDS 40.0%
TAGTTTAGATCAACCCGAAG+AGG - Chr3:48475013-48475032 MsG0380013941.01.T01:CDS 40.0%
TGCTACATCATAGGCACATT+GGG + Chr3:48474946-48474965 None:intergenic 40.0%
TTAATGCCCGAAAGTTGGAT+AGG - Chr3:48475179-48475198 MsG0380013941.01.T01:CDS 40.0%
! AATTCTCGGATTTTGCCTTG+GGG + Chr3:48475057-48475076 None:intergenic 40.0%
! GAATTCTCGGATTTTGCCTT+GGG + Chr3:48475058-48475077 None:intergenic 40.0%
!! GCATAAAAGTATTTGCCCCA+AGG - Chr3:48475039-48475058 MsG0380013941.01.T01:CDS 40.0%
ACACTAGCTAATAGTCCTCC+TGG + Chr3:48474969-48474988 None:intergenic 45.0%
AGCAATCGAATGCCAATCCT+CGG + Chr3:48474886-48474905 None:intergenic 45.0%
AGGCCATCCTATCCAACTTT+CGG + Chr3:48475189-48475208 None:intergenic 45.0%
GTGCTACATCATAGGCACAT+TGG + Chr3:48474947-48474966 None:intergenic 45.0%
! GGAATTCTCGGATTTTGCCT+TGG + Chr3:48475059-48475078 None:intergenic 45.0%
! TCTGCTTGGCTAGTCAAACA+CGG - Chr3:48474801-48474820 MsG0380013941.01.T01:CDS 45.0%
GCAATCGAATGCCAATCCTC+GGG + Chr3:48474885-48474904 None:intergenic 50.0%
GGCCATCCTATCCAACTTTC+GGG + Chr3:48475188-48475207 None:intergenic 50.0%
TGCAGGGTCATTTGTCTGCT+TGG - Chr3:48474787-48474806 MsG0380013941.01.T01:CDS 50.0%
TGCCCGAAAGTTGGATAGGA+TGG - Chr3:48475183-48475202 MsG0380013941.01.T01:CDS 50.0%
TGTGCCTATGATGTAGCACC+AGG - Chr3:48474948-48474967 MsG0380013941.01.T01:CDS 50.0%
!! TCCTCCTGGTGCTACATCAT+AGG + Chr3:48474955-48474974 None:intergenic 50.0%
GCCTATGATGTAGCACCAGG+AGG - Chr3:48474951-48474970 MsG0380013941.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48474786 48475262 48474786 ID=MsG0380013941.01;Name=MsG0380013941.01
Chr3 mRNA 48474786 48475262 48474786 ID=MsG0380013941.01.T01;Parent=MsG0380013941.01;Name=MsG0380013941.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|158
Chr3 exon 48474786 48475262 48474786 ID=MsG0380013941.01.T01:exon:11313;Parent=MsG0380013941.01.T01
Chr3 CDS 48474786 48475262 48474786 ID=MsG0380013941.01.T01:cds;Parent=MsG0380013941.01.T01
Gene Sequence

>MsG0380013941.01.T01

ATGCAGGGTCATTTGTCTGCTTGGCTAGTCAAACACGGAATAGTTCATAGATCTTTGGGTTTTGATTACCAAGGAATAGAGACTTTACAAATAAAGCCCGAGGATTGGCATTCGATTGCTGTTATTTTATATGTATATGGTTACAATTATCTACGTTCCCAATGTGCCTATGATGTAGCACCAGGAGGACTATTAGCTAGTGTCTATCATCTTACGAGAATAGAGTATAGTTTAGATCAACCCGAAGAGGTATGCATAAAAGTATTTGCCCCAAGGCAAAATCCGAGAATTCCTTCTATTTTCTGGGTTTGGAAAAGTGCTGATTTTCAAGAAAGGGAATCTTATGATATGTTAGGAATCTCTTATGATAATCATCCGCGTCTGAAACGTATTTTAATGCCCGAAAGTTGGATAGGATGGCCTTTGCGTAAAGATTATATCGCCCCCAATTTTTATGAAATACAGGATGCTCACTAA

Protein sequence

>MsG0380013941.01.T01

MQGHLSAWLVKHGIVHRSLGFDYQGIETLQIKPEDWHSIAVILYVYGYNYLRSQCAYDVAPGGLLASVYHLTRIEYSLDQPEEVCIKVFAPRQNPRIPSIFWVWKSADFQERESYDMLGISYDNHPRLKRILMPESWIGWPLRKDYIAPNFYEIQDAH*