AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014033.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014033.01.T01 MTR_4g050960 100.000 54 0 0 1 54 28 81 1.23e-35 114
MsG0380014033.01.T01 MTR_4g006120 94.444 54 3 0 1 54 28 81 3.51e-34 120
MsG0380014033.01.T01 MTR_7g021720 94.444 54 3 0 1 54 28 81 3.74e-34 120
MsG0380014033.01.T01 MTR_4g026420 86.842 38 5 0 16 53 51 88 3.65e-18 70.9
MsG0380014033.01.T01 MTR_0002s0170 100.000 24 0 0 1 24 75 98 3.91e-13 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014033.01.T01 ATCG01060 96.296 54 2 0 1 54 28 81 2.83e-33 108

Find 20 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAGTGTTCGGGTTTATTT+AGG 0.160330 3:-48702830 MsG0380014033.01.T01:CDS
CCCATACTTCGAGTTGTTTC+AGG 0.216040 3:+48702806 None:intergenic
CCAACACAGTCCTCTGTTCT+TGG 0.284080 3:+48702890 None:intergenic
GCCTGAAACAACTCGAAGTA+TGG 0.293148 3:-48702807 MsG0380014033.01.T01:CDS
TAAGTGTTCGGGTTTATTTA+GGG 0.345248 3:-48702829 MsG0380014033.01.T01:CDS
CGACGGATTTCTTAAGTGTT+CGG 0.352142 3:-48702841 MsG0380014033.01.T01:CDS
CAACACAGTCCTCTGTTCTT+GGG 0.362053 3:+48702891 None:intergenic
GACGGATTTCTTAAGTGTTC+GGG 0.373431 3:-48702840 MsG0380014033.01.T01:CDS
TGTATTAGAAATGATACCTT+GGG 0.506689 3:-48702945 None:intergenic
AGGACTGTGTTGGTTGTAAG+AGG 0.549741 3:-48702880 MsG0380014033.01.T01:CDS
TAAGAAATCCGTCGGACAAG+CGG 0.555225 3:+48702850 None:intergenic
ATGTATTAGAAATGATACCT+TGG 0.569899 3:-48702946 None:intergenic
AACACAGTCCTCTGTTCTTG+GGG 0.571622 3:+48702892 None:intergenic
GTGTGAGTCCGCTTGTCCGA+CGG 0.572513 3:-48702858 MsG0380014033.01.T01:CDS
CCTGAAACAACTCGAAGTAT+GGG 0.598749 3:-48702806 MsG0380014033.01.T01:CDS
TTAGCTTTACATCCATCCCA+AGG 0.604501 3:+48702929 None:intergenic
TTAGAAATGATACCTTGGGA+TGG 0.649544 3:-48702941 None:intergenic
AGCTTCTGCCCCAAGAACAG+AGG 0.655273 3:-48702900 MsG0380014033.01.T01:CDS
CCAAGAACAGAGGACTGTGT+TGG 0.685178 3:-48702890 MsG0380014033.01.T01:CDS
CGAACACTTAAGAAATCCGT+CGG 0.691774 3:+48702842 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAGTGTTCGGGTTTATTTA+GGG - Chr3:48702899-48702918 MsG0380014033.01.T01:CDS 30.0%
! TTAAGTGTTCGGGTTTATTT+AGG - Chr3:48702898-48702917 MsG0380014033.01.T01:CDS 30.0%
CCTGAAACAACTCGAAGTAT+GGG - Chr3:48702922-48702941 MsG0380014033.01.T01:CDS 40.0%
CGAACACTTAAGAAATCCGT+CGG + Chr3:48702889-48702908 None:intergenic 40.0%
! CGACGGATTTCTTAAGTGTT+CGG - Chr3:48702887-48702906 MsG0380014033.01.T01:CDS 40.0%
! GACGGATTTCTTAAGTGTTC+GGG - Chr3:48702888-48702907 MsG0380014033.01.T01:CDS 40.0%
!! TTAGCTTTACATCCATCCCA+AGG + Chr3:48702802-48702821 None:intergenic 40.0%
AACACAGTCCTCTGTTCTTG+GGG + Chr3:48702839-48702858 None:intergenic 45.0%
CAACACAGTCCTCTGTTCTT+GGG + Chr3:48702840-48702859 None:intergenic 45.0%
GCCTGAAACAACTCGAAGTA+TGG - Chr3:48702921-48702940 MsG0380014033.01.T01:CDS 45.0%
TAAGAAATCCGTCGGACAAG+CGG + Chr3:48702881-48702900 None:intergenic 45.0%
!! AGGACTGTGTTGGTTGTAAG+AGG - Chr3:48702848-48702867 MsG0380014033.01.T01:CDS 45.0%
!! CCCATACTTCGAGTTGTTTC+AGG + Chr3:48702925-48702944 None:intergenic 45.0%
CCAACACAGTCCTCTGTTCT+TGG + Chr3:48702841-48702860 None:intergenic 50.0%
CCAAGAACAGAGGACTGTGT+TGG - Chr3:48702838-48702857 MsG0380014033.01.T01:CDS 50.0%
AGCTTCTGCCCCAAGAACAG+AGG - Chr3:48702828-48702847 MsG0380014033.01.T01:CDS 55.0%
GTGTGAGTCCGCTTGTCCGA+CGG - Chr3:48702870-48702889 MsG0380014033.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48702793 48702957 48702793 ID=MsG0380014033.01;Name=MsG0380014033.01
Chr3 mRNA 48702793 48702957 48702793 ID=MsG0380014033.01.T01;Parent=MsG0380014033.01;Name=MsG0380014033.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|54
Chr3 exon 48702793 48702957 48702793 ID=MsG0380014033.01.T01:exon:35908;Parent=MsG0380014033.01.T01
Chr3 CDS 48702793 48702957 48702793 ID=MsG0380014033.01.T01:cds;Parent=MsG0380014033.01.T01
Chr3 mRNA 48702793 48702810 48702793 ID=MsG0380014033.01.T02;Parent=MsG0380014033.01;Name=MsG0380014033.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|5
Chr3 exon 48702793 48702810 48702793 ID=MsG0380014033.01.T02:exon:35909;Parent=MsG0380014033.01.T02
Chr3 CDS 48702793 48702810 48702793 ID=MsG0380014033.01.T02:cds;Parent=MsG0380014033.01.T02
Gene Sequence

>MsG0380014033.01.T01

ATGATACCTTGGGATGGATGTAAAGCTAAGCAAATAGCTTCTGCCCCAAGAACAGAGGACTGTGTTGGTTGTAAGAGGTGTGAGTCCGCTTGTCCGACGGATTTCTTAAGTGTTCGGGTTTATTTAGGGCCTGAAACAACTCGAAGTATGGGTCTAGCTTATTGA

>MsG0380014033.01.T02

ATGGGTCTAGCTTATTGA

Protein sequence

>MsG0380014033.01.T01

MIPWDGCKAKQIASAPRTEDCVGCKRCESACPTDFLSVRVYLGPETTRSMGLAY*

>MsG0380014033.01.T02

MGLAY*