AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014026.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014026.01.T01 MTR_7g021800 98.734 158 2 0 1 158 1 158 1.31e-116 326
MsG0380014026.01.T01 MTR_4g035040 98.734 158 2 0 1 158 1 158 1.31e-116 326
MsG0380014026.01.T01 MTR_0002s0630 87.261 157 20 0 2 158 162 318 6.98e-102 295
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014026.01.T01 ATCG00420 88.608 158 18 0 1 158 1 158 8.74e-106 299

Find 26 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAATAGTTCATAGATCTT+TGG 0.225646 3:+48680435 MsG0380014026.01.T01:CDS
GGCCATCCTATCCAACTTTC+GGG 0.254679 3:-48680799 None:intergenic
GGAATAGTTCATAGATCTTT+GGG 0.328701 3:+48680436 MsG0380014026.01.T01:CDS
AGGCCATCCTATCCAACTTT+CGG 0.340000 3:-48680800 None:intergenic
TTCCAAACCCAGAAAATAGA+AGG 0.366444 3:-48680691 None:intergenic
TTTGTAAAGTCTTTATTCCT+TGG 0.398764 3:-48680468 None:intergenic
TGCAGGGTCATTTGTCTGCT+TGG 0.409987 3:+48680401 MsG0380014026.01.T01:CDS
AGGGAATCTTATGATATGTT+AGG 0.415137 3:+48680733 MsG0380014026.01.T01:CDS
TGCTACATCATAGGCACATT+GGG 0.447727 3:-48680557 None:intergenic
TCCTCCTGGTGCTACATCAT+AGG 0.453068 3:-48680566 None:intergenic
TACAAATAAAGCCCGAGGAT+TGG 0.464528 3:+48680485 MsG0380014026.01.T01:CDS
CAGAAAATAGAAGGAATTCT+CGG 0.478109 3:-48680682 None:intergenic
AGCAATCGAATGCCAATCCT+CGG 0.513209 3:-48680497 None:intergenic
TGCCCGAAAGTTGGATAGGA+TGG 0.516198 3:+48680797 MsG0380014026.01.T01:CDS
GTGCTACATCATAGGCACAT+TGG 0.546406 3:-48680558 None:intergenic
TTAATGCCCGAAAGTTGGAT+AGG 0.556642 3:+48680793 MsG0380014026.01.T01:CDS
ACACTAGCTAATAGTCCTCC+TGG 0.563367 3:-48680580 None:intergenic
GCAATCGAATGCCAATCCTC+GGG 0.577805 3:-48680496 None:intergenic
TGTGCCTATGATGTAGCACC+AGG 0.591745 3:+48680562 MsG0380014026.01.T01:CDS
GCGATATAATCTTTACGCAA+AGG 0.602633 3:-48680820 None:intergenic
GCATAAAAGTATTTGCCCCA+AGG 0.613297 3:+48680653 MsG0380014026.01.T01:CDS
TAGTTTAGATCAACCCGAAG+AGG 0.615869 3:+48680627 MsG0380014026.01.T01:CDS
GACTTTACAAATAAAGCCCG+AGG 0.617607 3:+48680480 MsG0380014026.01.T01:CDS
ATTAAAATACGTTTCAGACG+CGG 0.619896 3:-48680775 None:intergenic
TCTGCTTGGCTAGTCAAACA+CGG 0.639180 3:+48680415 MsG0380014026.01.T01:CDS
GCCTATGATGTAGCACCAGG+AGG 0.695694 3:+48680565 MsG0380014026.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCCTGTATTTCATAAAAAT+TGG - Chr3:48680848-48680867 None:intergenic 20.0%
!! TCCTGTATTTCATAAAAATT+GGG - Chr3:48680847-48680866 None:intergenic 20.0%
!!! GCTGTTATTTTATATGTATA+TGG + Chr3:48680517-48680536 MsG0380014026.01.T01:CDS 20.0%
! CCTGTATTTCATAAAAATTG+GGG - Chr3:48680846-48680865 None:intergenic 25.0%
! CTGTATTTCATAAAAATTGG+GGG - Chr3:48680845-48680864 None:intergenic 25.0%
! TTTGTAAAGTCTTTATTCCT+TGG - Chr3:48680471-48680490 None:intergenic 25.0%
AGGGAATCTTATGATATGTT+AGG + Chr3:48680733-48680752 MsG0380014026.01.T01:CDS 30.0%
ATTAAAATACGTTTCAGACG+CGG - Chr3:48680778-48680797 None:intergenic 30.0%
CAGAAAATAGAAGGAATTCT+CGG - Chr3:48680685-48680704 None:intergenic 30.0%
GGAATAGTTCATAGATCTTT+GGG + Chr3:48680436-48680455 MsG0380014026.01.T01:CDS 30.0%
! CCCCAATTTTTATGAAATAC+AGG + Chr3:48680843-48680862 MsG0380014026.01.T01:CDS 30.0%
! GAGAATTCCTTCTATTTTCT+GGG + Chr3:48680684-48680703 MsG0380014026.01.T01:CDS 30.0%
! TACTTTTATGCATACCTCTT+CGG - Chr3:48680644-48680663 None:intergenic 30.0%
!!! AAAGTGCTGATTTTCAAGAA+AGG + Chr3:48680713-48680732 MsG0380014026.01.T01:CDS 30.0%
!!! AAGTGCTGATTTTCAAGAAA+GGG + Chr3:48680714-48680733 MsG0380014026.01.T01:CDS 30.0%
CGGAATAGTTCATAGATCTT+TGG + Chr3:48680435-48680454 MsG0380014026.01.T01:CDS 35.0%
GCGATATAATCTTTACGCAA+AGG - Chr3:48680823-48680842 None:intergenic 35.0%
TTCCAAACCCAGAAAATAGA+AGG - Chr3:48680694-48680713 None:intergenic 35.0%
! ACTTTTATGCATACCTCTTC+GGG - Chr3:48680643-48680662 None:intergenic 35.0%
! CGAGAATTCCTTCTATTTTC+TGG + Chr3:48680683-48680702 MsG0380014026.01.T01:CDS 35.0%
! TTCCTTCTATTTTCTGGGTT+TGG + Chr3:48680689-48680708 MsG0380014026.01.T01:CDS 35.0%
!!! GTATTTTAATGCCCGAAAGT+TGG + Chr3:48680788-48680807 MsG0380014026.01.T01:CDS 35.0%
!!! TCTTTGGGTTTTGATTACCA+AGG + Chr3:48680451-48680470 MsG0380014026.01.T01:CDS 35.0%
GACTTTACAAATAAAGCCCG+AGG + Chr3:48680480-48680499 MsG0380014026.01.T01:CDS 40.0%
TACAAATAAAGCCCGAGGAT+TGG + Chr3:48680485-48680504 MsG0380014026.01.T01:CDS 40.0%
TAGTTTAGATCAACCCGAAG+AGG + Chr3:48680627-48680646 MsG0380014026.01.T01:CDS 40.0%
TGCTACATCATAGGCACATT+GGG - Chr3:48680560-48680579 None:intergenic 40.0%
TTAATGCCCGAAAGTTGGAT+AGG + Chr3:48680793-48680812 MsG0380014026.01.T01:CDS 40.0%
! AATTCTCGGATTTTGCCTTG+GGG - Chr3:48680671-48680690 None:intergenic 40.0%
! GAATTCTCGGATTTTGCCTT+GGG - Chr3:48680672-48680691 None:intergenic 40.0%
!! GCATAAAAGTATTTGCCCCA+AGG + Chr3:48680653-48680672 MsG0380014026.01.T01:CDS 40.0%
ACACTAGCTAATAGTCCTCC+TGG - Chr3:48680583-48680602 None:intergenic 45.0%
AGCAATCGAATGCCAATCCT+CGG - Chr3:48680500-48680519 None:intergenic 45.0%
AGGCCATCCTATCCAACTTT+CGG - Chr3:48680803-48680822 None:intergenic 45.0%
GTGCTACATCATAGGCACAT+TGG - Chr3:48680561-48680580 None:intergenic 45.0%
! GGAATTCTCGGATTTTGCCT+TGG - Chr3:48680673-48680692 None:intergenic 45.0%
! TCTGCTTGGCTAGTCAAACA+CGG + Chr3:48680415-48680434 MsG0380014026.01.T01:CDS 45.0%
GCAATCGAATGCCAATCCTC+GGG - Chr3:48680499-48680518 None:intergenic 50.0%
GGCCATCCTATCCAACTTTC+GGG - Chr3:48680802-48680821 None:intergenic 50.0%
TGCAGGGTCATTTGTCTGCT+TGG + Chr3:48680401-48680420 MsG0380014026.01.T01:CDS 50.0%
TGCCCGAAAGTTGGATAGGA+TGG + Chr3:48680797-48680816 MsG0380014026.01.T01:CDS 50.0%
TGTGCCTATGATGTAGCACC+AGG + Chr3:48680562-48680581 MsG0380014026.01.T01:CDS 50.0%
!! TCCTCCTGGTGCTACATCAT+AGG - Chr3:48680569-48680588 None:intergenic 50.0%
GCCTATGATGTAGCACCAGG+AGG + Chr3:48680565-48680584 MsG0380014026.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48680400 48680876 48680400 ID=MsG0380014026.01;Name=MsG0380014026.01
Chr3 mRNA 48680400 48680876 48680400 ID=MsG0380014026.01.T01;Parent=MsG0380014026.01;Name=MsG0380014026.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|158
Chr3 exon 48680400 48680876 48680400 ID=MsG0380014026.01.T01:exon:8541;Parent=MsG0380014026.01.T01
Chr3 CDS 48680400 48680876 48680400 ID=MsG0380014026.01.T01:cds;Parent=MsG0380014026.01.T01
Gene Sequence

>MsG0380014026.01.T01

ATGCAGGGTCATTTGTCTGCTTGGCTAGTCAAACACGGAATAGTTCATAGATCTTTGGGTTTTGATTACCAAGGAATAAAGACTTTACAAATAAAGCCCGAGGATTGGCATTCGATTGCTGTTATTTTATATGTATATGGTTACAATTATCTACGTTCCCAATGTGCCTATGATGTAGCACCAGGAGGACTATTAGCTAGTGTCTATCATCTTACGAGAATAGAGTATAGTTTAGATCAACCCGAAGAGGTATGCATAAAAGTATTTGCCCCAAGGCAAAATCCGAGAATTCCTTCTATTTTCTGGGTTTGGAAAAGTGCTGATTTTCAAGAAAGGGAATCTTATGATATGTTAGGAATCTCTTATGATAATCATCCGCGTCTGAAACGTATTTTAATGCCCGAAAGTTGGATAGGATGGCCTTTGCGTAAAGATTATATCGCCCCCAATTTTTATGAAATACAGGATGCTCACTAA

Protein sequence

>MsG0380014026.01.T01

MQGHLSAWLVKHGIVHRSLGFDYQGIKTLQIKPEDWHSIAVILYVYGYNYLRSQCAYDVAPGGLLASVYHLTRIEYSLDQPEEVCIKVFAPRQNPRIPSIFWVWKSADFQERESYDMLGISYDNHPRLKRILMPESWIGWPLRKDYIAPNFYEIQDAH*