Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004507.01.T01 | XP_003591018.1 | 94.444 | 90 | 5 | 0 | 1 | 90 | 653 | 742 | 3.03E-49 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004507.01.T01 | G7I7D3 | 94.444 | 90 | 5 | 0 | 1 | 90 | 653 | 742 | 1.45e-49 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048302.01 | MsG0180004507.01 | -0.810179 | 1.311188e-50 | 7.849748e-48 |
| MsG0080048692.01 | MsG0180004507.01 | 0.825707 | 4.046879e-54 | 3.717192e-51 |
| MsG0080048693.01 | MsG0180004507.01 | 0.800119 | 1.673460e-48 | 7.729231e-46 |
| MsG0080049015.01 | MsG0180004507.01 | 0.807109 | 5.934403e-50 | 3.277072e-47 |
| MsG0080049076.01 | MsG0180004507.01 | 0.825105 | 5.619099e-54 | 5.073523e-51 |
| MsG0180002439.01 | MsG0180004507.01 | 0.824721 | 6.921927e-54 | 6.182182e-51 |
| MsG0180002546.01 | MsG0180004507.01 | 0.808697 | 2.726358e-50 | 1.569366e-47 |
| MsG0180004506.01 | MsG0180004507.01 | 0.863656 | 2.130069e-64 | 6.507521e-61 |
| MsG0180004507.01 | MsG0380013100.01 | -0.813175 | 2.923172e-51 | 1.895221e-48 |
| MsG0180004507.01 | MsG0380013278.01 | -0.802796 | 4.733250e-49 | 2.339916e-46 |
| MsG0180004507.01 | MsG0380013890.01 | 0.805784 | 1.129588e-49 | 6.029627e-47 |
| MsG0180004507.01 | MsG0380013892.01 | 0.810952 | 8.925111e-51 | 5.454818e-48 |
| MsG0180004507.01 | MsG0380013921.01 | 0.805549 | 1.265418e-49 | 6.713624e-47 |
| MsG0180004507.01 | MsG0380013940.01 | 0.823845 | 1.112232e-53 | 9.688049e-51 |
| MsG0180004507.01 | MsG0380013943.01 | 0.828472 | 8.820281e-55 | 8.775855e-52 |
| MsG0180004507.01 | MsG0380013945.01 | 0.834575 | 2.768293e-56 | 3.298995e-53 |
| MsG0180004507.01 | MsG0380013953.01 | 0.838249 | 3.216719e-57 | 4.283226e-54 |
| MsG0180004507.01 | MsG0380013954.01 | 0.831366 | 1.738548e-55 | 1.882614e-52 |
| MsG0180004507.01 | MsG0380013957.01 | 0.819615 | 1.058107e-52 | 8.182393e-50 |
| MsG0180004507.01 | MsG0380013966.01 | 0.807853 | 4.125279e-50 | 2.322601e-47 |
| MsG0180004507.01 | MsG0380013967.01 | 0.822116 | 2.814237e-53 | 2.333857e-50 |
| MsG0180004507.01 | MsG0380013971.01 | 0.809624 | 1.725679e-50 | 1.018298e-47 |
| MsG0180004507.01 | MsG0380013977.01 | 0.817109 | 3.909924e-52 | 2.821813e-49 |
| MsG0180004507.01 | MsG0380014005.01 | 0.816604 | 5.075166e-52 | 3.612633e-49 |
| MsG0180004507.01 | MsG0380014006.01 | 0.837514 | 4.970830e-57 | 6.472627e-54 |
| MsG0180004507.01 | MsG0380014020.01 | 0.816351 | 5.784796e-52 | 4.089381e-49 |
| MsG0180004507.01 | MsG0380014021.01 | 0.801870 | 7.340930e-49 | 3.544492e-46 |
| MsG0180004507.01 | MsG0380014026.01 | 0.801974 | 6.989753e-49 | 3.383489e-46 |
| MsG0180004507.01 | MsG0380014032.01 | 0.844091 | 9.372148e-59 | 1.496688e-55 |
| MsG0180004507.01 | MsG0380014033.01 | 0.821274 | 4.404571e-53 | 3.566870e-50 |
| MsG0180004507.01 | MsG0380015217.01 | -0.850521 | 1.608943e-60 | 3.159958e-57 |
| MsG0180004507.01 | MsG0380016270.01 | -0.808160 | 3.549414e-50 | 2.014788e-47 |
| MsG0180004507.01 | MsG0380017203.01 | -0.812695 | 3.725282e-51 | 2.384707e-48 |
| MsG0180004507.01 | MsG0380017972.01 | 0.811034 | 8.568024e-51 | 5.247614e-48 |
| MsG0180004507.01 | MsG0480019107.01 | -0.814677 | 1.364248e-51 | 9.213074e-49 |
| MsG0180004507.01 | MsG0480021912.01 | 0.816047 | 6.761317e-52 | 4.740273e-49 |
| MsG0180004507.01 | MsG0580025145.01 | -0.800715 | 1.265553e-48 | 5.934036e-46 |
| MsG0180004507.01 | MsG0680035842.01 | -0.822617 | 2.152015e-53 | 1.810667e-50 |
| MsG0180004507.01 | MsG0780040060.01 | -0.817303 | 3.537402e-52 | 2.566519e-49 |
| MsG0180004507.01 | MsG0780041007.01 | 0.844303 | 8.216692e-59 | 1.320907e-55 |
| MsG0180004507.01 | MsG0880042810.01 | -0.806508 | 7.949893e-50 | 4.322711e-47 |
| MsG0180004507.01 | MsG0880043537.01 | -0.806886 | 6.615549e-50 | 3.632307e-47 |
| MsG0180004507.01 | MsG0880044855.01 | -0.801135 | 1.038588e-48 | 4.922401e-46 |
| MsG0180004507.01 | MsG0880045861.01 | -0.817943 | 2.536994e-52 | 1.873261e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004507.01.T01 | MTR_1g081330 | 94.444 | 90 | 5 | 0 | 1 | 90 | 653 | 742 | 3.67e-53 | 176 |
| MsG0180004507.01.T01 | MTR_1g081330 | 94.444 | 90 | 5 | 0 | 1 | 90 | 568 | 657 | 5.61e-53 | 174 |
| MsG0180004507.01.T01 | MTR_7g114680 | 45.455 | 88 | 44 | 2 | 3 | 90 | 629 | 712 | 9.92e-15 | 68.2 |
| MsG0180004507.01.T01 | MTR_7g114680 | 45.455 | 88 | 44 | 2 | 3 | 90 | 643 | 726 | 1.04e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCCGATTCAGACGCCAAAA+TGG | 0.346194 | 1:+78965279 | MsG0180004507.01.T01:CDS |
| TATATATCAACAGCATCAAC+AGG | 0.377233 | 1:-78965412 | None:intergenic |
| TGCAACCTGTCATTAATAAC+TGG | 0.390514 | 1:+78965215 | MsG0180004507.01.T01:CDS |
| TTGTGAGGCGATGGAATCCC+CGG | 0.443423 | 1:+78965309 | MsG0180004507.01.T01:CDS |
| GAGCTCCAGTTATTAATGAC+AGG | 0.515116 | 1:-78965220 | None:intergenic |
| GTAACCATCATACCTTCTCC+CGG | 0.532499 | 1:-78965328 | None:intergenic |
| TTGCAAGCGAATCACATTGT+CGG | 0.549721 | 1:-78965197 | None:intergenic |
| TAACCATCATACCTTCTCCC+GGG | 0.552562 | 1:-78965327 | None:intergenic |
| TGTTTCCGCACTCTCTGCAC+AGG | 0.555953 | 1:+78965377 | MsG0180004507.01.T01:CDS |
| GCGATGGAATCCCCGGGAGA+AGG | 0.558841 | 1:+78965316 | MsG0180004507.01.T01:CDS |
| TTGAATGTTTCTGTCAACGA+AGG | 0.589032 | 1:-78965169 | None:intergenic |
| ATCCCCGGGAGAAGGTATGA+TGG | 0.606624 | 1:+78965324 | MsG0180004507.01.T01:CDS |
| TGTGAGGCGATGGAATCCCC+GGG | 0.632344 | 1:+78965310 | MsG0180004507.01.T01:CDS |
| TCGTTGACAGAAACATTCAA+AGG | 0.640568 | 1:+78965172 | MsG0180004507.01.T01:CDS |
| AACCATCATACCTTCTCCCG+GGG | 0.648735 | 1:-78965326 | None:intergenic |
| ACTGTCCTGTGCAGAGAGTG+CGG | 0.687084 | 1:-78965382 | None:intergenic |
| ATATATCAACAGCATCAACA+GGG | 0.712378 | 1:-78965411 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATATATCAACAGCATCAACA+GGG | - | Chr1:78965414-78965433 | None:intergenic | 30.0% | |
| TATATATCAACAGCATCAAC+AGG | - | Chr1:78965415-78965434 | None:intergenic | 30.0% | |
| CAAAATGGCCAAAAATTGTG+AGG | + | Chr1:78965294-78965313 | MsG0180004507.01.T01:CDS | 35.0% | |
| TCGTTGACAGAAACATTCAA+AGG | + | Chr1:78965172-78965191 | MsG0180004507.01.T01:CDS | 35.0% | |
| TGCAACCTGTCATTAATAAC+TGG | + | Chr1:78965215-78965234 | MsG0180004507.01.T01:CDS | 35.0% | |
| ! | ACTGCAAAGAGTTTTTACAG+TGG | + | Chr1:78965349-78965368 | MsG0180004507.01.T01:CDS | 35.0% |
| ! | TTGAATGTTTCTGTCAACGA+AGG | - | Chr1:78965172-78965191 | None:intergenic | 35.0% |
| !!! | CTCACAATTTTTGGCCATTT+TGG | - | Chr1:78965296-78965315 | None:intergenic | 35.0% |
| GAGCTCCAGTTATTAATGAC+AGG | - | Chr1:78965223-78965242 | None:intergenic | 40.0% | |
| TTGCAAGCGAATCACATTGT+CGG | - | Chr1:78965200-78965219 | None:intergenic | 40.0% | |
| ! | TGCTTTTATGAACACGAGGA+CGG | - | Chr1:78965258-78965277 | None:intergenic | 40.0% |
| ! | TTCCATCGCCTCACAATTTT+TGG | - | Chr1:78965305-78965324 | None:intergenic | 40.0% |
| GTAACCATCATACCTTCTCC+CGG | - | Chr1:78965331-78965350 | None:intergenic | 45.0% | |
| TAACCATCATACCTTCTCCC+GGG | - | Chr1:78965330-78965349 | None:intergenic | 45.0% | |
| AACCATCATACCTTCTCCCG+GGG | - | Chr1:78965329-78965348 | None:intergenic | 50.0% | |
| GGCCAAAAATTGTGAGGCGA+TGG | + | Chr1:78965300-78965319 | MsG0180004507.01.T01:CDS | 50.0% | |
| !! | CCATTTTGGCGTCTGAATCG+GGG | - | Chr1:78965282-78965301 | None:intergenic | 50.0% |
| !! | GCCATTTTGGCGTCTGAATC+GGG | - | Chr1:78965283-78965302 | None:intergenic | 50.0% |
| !! | GGCCATTTTGGCGTCTGAAT+CGG | - | Chr1:78965284-78965303 | None:intergenic | 50.0% |
| !!! | GGGCTGCTTTTATGAACACG+AGG | - | Chr1:78965262-78965281 | None:intergenic | 50.0% |
| ACTGTCCTGTGCAGAGAGTG+CGG | - | Chr1:78965385-78965404 | None:intergenic | 55.0% | |
| CCCCGATTCAGACGCCAAAA+TGG | + | Chr1:78965279-78965298 | MsG0180004507.01.T01:CDS | 55.0% | |
| TGTTTCCGCACTCTCTGCAC+AGG | + | Chr1:78965377-78965396 | MsG0180004507.01.T01:CDS | 55.0% | |
| TTGTGAGGCGATGGAATCCC+CGG | + | Chr1:78965309-78965328 | MsG0180004507.01.T01:CDS | 55.0% | |
| ! | ATCCCCGGGAGAAGGTATGA+TGG | + | Chr1:78965324-78965343 | MsG0180004507.01.T01:CDS | 55.0% |
| TGTGAGGCGATGGAATCCCC+GGG | + | Chr1:78965310-78965329 | MsG0180004507.01.T01:CDS | 60.0% | |
| GCGATGGAATCCCCGGGAGA+AGG | + | Chr1:78965316-78965335 | MsG0180004507.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 78965163 | 78965435 | 78965163 | ID=MsG0180004507.01;Name=MsG0180004507.01 |
| Chr1 | mRNA | 78965163 | 78965435 | 78965163 | ID=MsG0180004507.01.T01;Parent=MsG0180004507.01;Name=MsG0180004507.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|90 |
| Chr1 | exon | 78965163 | 78965435 | 78965163 | ID=MsG0180004507.01.T01:exon:15261;Parent=MsG0180004507.01.T01 |
| Chr1 | CDS | 78965163 | 78965435 | 78965163 | ID=MsG0180004507.01.T01:cds;Parent=MsG0180004507.01.T01 |
| Gene Sequence |
| Protein sequence |
