AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013943.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013943.01.T01 MTR_4g050850 62.500 128 39 1 1 119 1 128 6.87e-45 143
MsG0380013943.01.T01 MTR_0002s0700 95.714 70 3 0 1 70 1 70 1.90e-43 137
MsG0380013943.01.T01 MTR_5g055290 97.143 70 2 0 1 70 1 70 3.71e-43 137
MsG0380013943.01.T01 MTR_6g007780 97.297 37 1 0 25 61 76 112 3.53e-19 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013943.01.T01 ATCG00580 97.143 70 2 0 1 70 1 70 3.41e-44 139

Find 39 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACTGGCCGTTTGATTCTT+TGG 0.203448 3:-48481090 MsG0380013943.01.T01:CDS
GGTTTAGCTTACGATGTATT+TGG 0.244623 3:-48481166 MsG0380013943.01.T01:CDS
TTAATTCATCCTATTATTAC+AGG 0.261070 3:+48480508 None:intergenic
AGGATTCCTCTTTGGATAAT+AGG 0.264351 3:-48480606 MsG0380013943.01.T01:CDS
ATCAAACGGCCAGTTATTAA+TGG 0.292121 3:+48481097 None:intergenic
CTACAGGAAGGATTCCTCTT+TGG 0.328597 3:-48480614 MsG0380013943.01.T01:CDS
ATAACCAACCCGCAATGAAT+AGG 0.348030 3:+48481200 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 3:-48481249 MsG0380013943.01.T01:CDS
TATGTGGAGCTCGGCATGTC+TGG 0.349487 3:-48481301 None:intergenic
GGGATCATCCCTGTAATAAT+AGG 0.349809 3:-48480517 MsG0380013943.01.T01:CDS
GTGGAGGAAAGTGGGATAAA+TGG 0.355855 3:-48480643 MsG0380013943.01.T01:intron
TATGGTTCATATTCCGGATT+GGG 0.369794 3:-48480537 MsG0380013943.01.T01:CDS
ATACCCTCCCTATTCATTGC+GGG 0.391873 3:-48481208 MsG0380013943.01.T01:CDS
TAACCAACCCGCAATGAATA+GGG 0.406851 3:+48481201 None:intergenic
CTTTCTGTAAAATACTCGTT+TGG 0.412027 3:+48481133 None:intergenic
CAAGGAATTCCATTAATAAC+TGG 0.412082 3:-48481106 MsG0380013943.01.T01:CDS
TGGATAATAGGTACTGTAAC+AGG 0.413541 3:-48480594 MsG0380013943.01.T01:CDS
AATAATCCAGTATCGAATAC+TGG 0.423687 3:+48481243 None:intergenic
TTATTAATGGAATTCCTTGT+CGG 0.434610 3:+48481110 None:intergenic
TTATGGTTCATATTCCGGAT+TGG 0.452450 3:-48480538 MsG0380013943.01.T01:CDS
GGATAAATGGCCGATACTAC+AGG 0.493573 3:-48480630 MsG0380013943.01.T01:CDS
TGATCAAATGTGGAGGAAAG+TGG 0.506780 3:-48480652 MsG0380013943.01.T01:intron
CTCGGCATGTCTGGAAGCAC+AGG 0.508926 3:-48481292 None:intergenic
ATTGTCGTGATCGGTTTAGT+TGG 0.509440 3:-48480570 MsG0380013943.01.T01:CDS
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 3:-48481187 MsG0380013943.01.T01:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 3:-48481188 MsG0380013943.01.T01:CDS
CCTCCCTATTCATTGCGGGT+TGG 0.536548 3:-48481204 MsG0380013943.01.T01:CDS
AGGAATCCTTCCTGTAGTAT+CGG 0.545192 3:+48480620 None:intergenic
GTAAAATACTCGTTTGGACG+AGG 0.550593 3:+48481139 None:intergenic
AGTTGTTCCAAAGAATCAAA+CGG 0.569843 3:+48481083 None:intergenic
GATCAAATGTGGAGGAAAGT+GGG 0.574911 3:-48480651 MsG0380013943.01.T01:intron
GTAACAGGTATTGTCGTGAT+CGG 0.578172 3:-48480579 MsG0380013943.01.T01:CDS
TTACAGGGATGATCCCAATC+CGG 0.579951 3:+48480524 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 3:-48481209 MsG0380013943.01.T01:CDS
TAATTCATCCTATTATTACA+GGG 0.594087 3:+48480509 None:intergenic
ACAGTACCTATTATCCAAAG+AGG 0.618357 3:+48480600 None:intergenic
CAACCCGCAATGAATAGGGA+GGG 0.665638 3:+48481205 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 3:+48481204 None:intergenic
AAATGGCCGATACTACAGGA+AGG 0.697836 3:-48480626 MsG0380013943.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATT+AGG - Chr3:48481046-48481065 MsG0380013943.01.T01:intron 0.0%
!! ATAATTATCTATGACTGTTT+AGG - Chr3:48481103-48481122 MsG0380013943.01.T01:CDS 20.0%
!! CTTCAATTAATAGAAGAAAA+GGG - Chr3:48481021-48481040 MsG0380013943.01.T01:intron 20.0%
!! TCTTCAATTAATAGAAGAAA+AGG - Chr3:48481020-48481039 MsG0380013943.01.T01:intron 20.0%
!!! AATTCAACATTTTGTTCATT+TGG + Chr3:48480932-48480951 None:intergenic 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG + Chr3:48480931-48480950 None:intergenic 20.0%
!!! TGAATTTAGTAGATCTTTTT+AGG - Chr3:48480742-48480761 MsG0380013943.01.T01:intron 20.0%
!!! TTAGTTGGTATTTTCTTTTA+TGG - Chr3:48481244-48481263 MsG0380013943.01.T01:CDS 20.0%
! ATATTGATCCCAAAAAAAGA+CGG + Chr3:48480846-48480865 None:intergenic 25.0%
! TTATTAATGGAATTCCTTGT+CGG + Chr3:48480692-48480711 None:intergenic 25.0%
!! TTCTTTTATGGTTCATATTC+CGG - Chr3:48481256-48481275 MsG0380013943.01.T01:CDS 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG - Chr3:48480745-48480764 MsG0380013943.01.T01:intron 25.0%
AACCATCGTACTGTAAAAAT+GGG + Chr3:48480797-48480816 None:intergenic 30.0%
AGTTGTTCCAAAGAATCAAA+CGG + Chr3:48480719-48480738 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG - Chr3:48480693-48480712 MsG0380013943.01.T01:intron 30.0%
CTTTCTGTAAAATACTCGTT+TGG + Chr3:48480669-48480688 None:intergenic 30.0%
TTATTACCAGTATTCGATAC+TGG - Chr3:48480550-48480569 MsG0380013943.01.T01:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG + Chr3:48480559-48480578 None:intergenic 30.0%
ACAGTACCTATTATCCAAAG+AGG + Chr3:48481202-48481221 None:intergenic 35.0%
ACTGACTACTTGATCAAATG+TGG - Chr3:48481137-48481156 MsG0380013943.01.T01:CDS 35.0%
AGGAATTCTCTTATCCCATT+CGG - Chr3:48481066-48481085 MsG0380013943.01.T01:intron 35.0%
AGGATTCCTCTTTGGATAAT+AGG - Chr3:48481193-48481212 MsG0380013943.01.T01:CDS 35.0%
ATCAAACGGCCAGTTATTAA+TGG + Chr3:48480705-48480724 None:intergenic 35.0%
CAACCATCGTACTGTAAAAA+TGG + Chr3:48480798-48480817 None:intergenic 35.0%
GAAATGATATCCTTCCGAAT+GGG + Chr3:48481083-48481102 None:intergenic 35.0%
TATGGTTCATATTCCGGATT+GGG - Chr3:48481262-48481281 MsG0380013943.01.T01:CDS 35.0%
TCGTACTGTAAAAATGGGAT+AGG + Chr3:48480792-48480811 None:intergenic 35.0%
TGAAATGATATCCTTCCGAA+TGG + Chr3:48481084-48481103 None:intergenic 35.0%
TTATGGTTCATATTCCGGAT+TGG - Chr3:48481261-48481280 MsG0380013943.01.T01:CDS 35.0%
! ATTCGGATTAGGTTTATCGT+TGG + Chr3:48480885-48480904 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG - Chr3:48480796-48480815 MsG0380013943.01.T01:intron 35.0%
! GGTTTAGCTTACGATGTATT+TGG - Chr3:48480633-48480652 MsG0380013943.01.T01:CDS 35.0%
! GTAGCTCTATAATTCGGATT+AGG + Chr3:48480896-48480915 None:intergenic 35.0%
! TATTTTACAGAAAGCCGACA+AGG - Chr3:48480675-48480694 MsG0380013943.01.T01:intron 35.0%
! TGGATAATAGGTACTGTAAC+AGG - Chr3:48481205-48481224 MsG0380013943.01.T01:CDS 35.0%
! TGTGTCGTAGCTCTATAATT+CGG + Chr3:48480902-48480921 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG - Chr3:48480792-48480811 MsG0380013943.01.T01:intron 35.0%
AATGGGATAGGTCCTATCTA+TGG + Chr3:48480780-48480799 None:intergenic 40.0%
AGGAATCCTTCCTGTAGTAT+CGG + Chr3:48481182-48481201 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG + Chr3:48480602-48480621 None:intergenic 40.0%
ATTCTCTTATCCCATTCGGA+AGG - Chr3:48481070-48481089 MsG0380013943.01.T01:intron 40.0%
GACTACTTGATCAAATGTGG+AGG - Chr3:48481140-48481159 MsG0380013943.01.T01:CDS 40.0%
GATCAAATGTGGAGGAAAGT+GGG - Chr3:48481148-48481167 MsG0380013943.01.T01:CDS 40.0%
GGGATCATCCCTGTAATAAT+AGG - Chr3:48481282-48481301 MsG0380013943.01.T01:CDS 40.0%
GTAAAATACTCGTTTGGACG+AGG + Chr3:48480663-48480682 None:intergenic 40.0%
TAACCAACCCGCAATGAATA+GGG + Chr3:48480601-48480620 None:intergenic 40.0%
TAACTGGCCGTTTGATTCTT+TGG - Chr3:48480709-48480728 MsG0380013943.01.T01:intron 40.0%
TAATAAACCCCAGTAGAGAC+TGG + Chr3:48480963-48480982 None:intergenic 40.0%
TAGGTCCTATCTATGGTCAT+TGG + Chr3:48480773-48480792 None:intergenic 40.0%
TATACCCTCCCTATTCATTG+CGG - Chr3:48480590-48480609 MsG0380013943.01.T01:CDS 40.0%
TGATCAAATGTGGAGGAAAG+TGG - Chr3:48481147-48481166 MsG0380013943.01.T01:CDS 40.0%
TGATCCCAAAAAAAGACGGT+GGG + Chr3:48480842-48480861 None:intergenic 40.0%
TTGATCCCAAAAAAAGACGG+TGG + Chr3:48480843-48480862 None:intergenic 40.0%
! ATTGTCGTGATCGGTTTAGT+TGG - Chr3:48481229-48481248 MsG0380013943.01.T01:CDS 40.0%
! GTAACAGGTATTGTCGTGAT+CGG - Chr3:48481220-48481239 MsG0380013943.01.T01:CDS 40.0%
AAATGGCCGATACTACAGGA+AGG - Chr3:48481173-48481192 MsG0380013943.01.T01:CDS 45.0%
AATCGTACCAGTCTCTACTG+GGG - Chr3:48480953-48480972 MsG0380013943.01.T01:intron 45.0%
AGGCTCCAATGACCATAGAT+AGG - Chr3:48480765-48480784 MsG0380013943.01.T01:intron 45.0%
ATACCCTCCCTATTCATTGC+GGG - Chr3:48480591-48480610 MsG0380013943.01.T01:CDS 45.0%
CTACAGGAAGGATTCCTCTT+TGG - Chr3:48481185-48481204 MsG0380013943.01.T01:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG - Chr3:48480952-48480971 MsG0380013943.01.T01:intron 45.0%
GGATAAATGGCCGATACTAC+AGG - Chr3:48481169-48481188 MsG0380013943.01.T01:CDS 45.0%
GTGGAGGAAAGTGGGATAAA+TGG - Chr3:48481156-48481175 MsG0380013943.01.T01:CDS 45.0%
TGAATCGTACCAGTCTCTAC+TGG - Chr3:48480951-48480970 MsG0380013943.01.T01:intron 45.0%
TTACAGGGATGATCCCAATC+CGG + Chr3:48481278-48481297 None:intergenic 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG - Chr3:48480612-48480631 MsG0380013943.01.T01:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG - Chr3:48480611-48480630 MsG0380013943.01.T01:CDS 45.0%
!!! GGTACCCACCGTCTTTTTTT+GGG - Chr3:48480835-48480854 MsG0380013943.01.T01:intron 45.0%
CAACCCGCAATGAATAGGGA+GGG + Chr3:48480597-48480616 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG - Chr3:48480806-48480825 MsG0380013943.01.T01:intron 50.0%
!!! CGGTACCCACCGTCTTTTTT+TGG - Chr3:48480834-48480853 MsG0380013943.01.T01:intron 50.0%
CCAACCCGCAATGAATAGGG+AGG + Chr3:48480598-48480617 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG - Chr3:48480595-48480614 MsG0380013943.01.T01:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG - Chr3:48480814-48480833 MsG0380013943.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48480513 48481308 48480513 ID=MsG0380013943.01;Name=MsG0380013943.01
Chr3 mRNA 48480513 48481308 48480513 ID=MsG0380013943.01.T01;Parent=MsG0380013943.01;Name=MsG0380013943.01.T01;_AED=0.50;_eAED=0.69;_QI=0|0|0|1|0|0|2|0|127
Chr3 exon 48481072 48481308 48481072 ID=MsG0380013943.01.T01:exon:9359;Parent=MsG0380013943.01.T01
Chr3 exon 48480513 48480659 48480513 ID=MsG0380013943.01.T01:exon:9358;Parent=MsG0380013943.01.T01
Chr3 CDS 48481072 48481308 48481072 ID=MsG0380013943.01.T01:cds;Parent=MsG0380013943.01.T01
Chr3 CDS 48480513 48480659 48480513 ID=MsG0380013943.01.T01:cds;Parent=MsG0380013943.01.T01
Gene Sequence

>MsG0380013943.01.T01

ATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCTCGTCCAAACGAGTATTTTACAGAAAGCCGACAAGGAATTCCATTAATAACTGGCCGTTTGATTCTTTGGAACAACTCGATGAATTTAGAAAGTGGGATAAATGGCCGATACTACAGGAAGGATTCCTCTTTGGATAATAGGTACTGTAACAGGTATTGTCGTGATCGGTTTAGTTGGTATTTTCTTTTATGGTTCATATTCCGGATTGGGATCATCCCTGTAATAATAGGATGA

Protein sequence

>MsG0380013943.01.T01

MSGSTGERSFADIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPRPNEYFTESRQGIPLITGRLILWNNSMNLESGINGRYYRKDSSLDNRYCNRYCRDRFSWYFLLWFIFRIGIIPVIIG*