AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004977.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004977.01.T01 MTR_1g090687 96.651 209 7 0 1 209 38 246 1.76e-148 430
MsG0180004977.01.T01 MTR_1g090683 77.033 209 45 1 1 209 49 254 2.52e-112 337
MsG0180004977.01.T01 MTR_8g020780 45.355 183 97 1 1 180 49 231 1.26e-51 178
MsG0180004977.01.T01 MTR_1g022060 41.872 203 109 2 2 201 31 227 5.93e-41 148
MsG0180004977.01.T01 MTR_2g048300 39.130 138 82 1 1 136 31 168 2.39e-28 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004977.01.T01 AT5G45470 51.176 170 78 3 2 167 31 199 3.91e-45 160
MsG0180004977.01.T01 AT5G45460 49.112 169 81 3 3 167 32 199 7.81e-43 153
MsG0180004977.01.T01 AT5G45540 46.746 169 85 2 2 166 31 198 2.94e-42 152
MsG0180004977.01.T01 AT5G45480 42.439 205 106 4 3 201 32 230 3.04e-40 146
MsG0180004977.01.T01 AT5G45530 43.860 171 85 3 3 166 31 197 1.45e-35 133
MsG0180004977.01.T01 AT4G19090 50.495 101 49 1 55 155 82 181 1.93e-24 101

Find 58 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATGATCTTTAGTGTCTT+TGG 0.208303 1:+85124099 None:intergenic
TTGGTTCTCCCTTGAGTATT+AGG 0.249278 1:+85123662 None:intergenic
AAAGGCTAAAATTGTTGATA+TGG 0.257398 1:-85124174 MsG0180004977.01.T01:CDS
CATAAACTGCAACTGCTAAT+TGG 0.259955 1:+85123942 None:intergenic
CCATTTCTCCTTGTGCATCT+TGG 0.274921 1:-85124040 MsG0180004977.01.T01:CDS
TGAAACTACTCCCTTAGTTT+TGG 0.276491 1:-85123687 MsG0180004977.01.T01:CDS
CACTTATCTGTCAGCTGATT+GGG 0.277339 1:-85124149 MsG0180004977.01.T01:CDS
AGTTTCATTAATTTCATCTA+AGG 0.293820 1:+85123703 None:intergenic
TCACGTAAAGCTTGACTATC+TGG 0.300740 1:+85123620 None:intergenic
TCCATGAATTTAGCATAGTT+TGG 0.319706 1:+85123764 None:intergenic
ATTATTCCTGCCACGACAAT+AGG 0.320930 1:+85123872 None:intergenic
TTGCAGTTTATGTTGTTTAT+CGG 0.324974 1:-85123931 MsG0180004977.01.T01:CDS
TCACTTATCTGTCAGCTGAT+TGG 0.326710 1:-85124150 MsG0180004977.01.T01:CDS
GGAAGCATGTAGTTGATAAA+AGG 0.327912 1:-85124192 MsG0180004977.01.T01:CDS
GCATAGTTTGGTCCTGGGTC+TGG 0.329432 1:+85123776 None:intergenic
AGACACCAACTTCGTAATTA+TGG 0.335596 1:-85124074 MsG0180004977.01.T01:CDS
TTGGGTTGCTACCGTTGCTC+TGG 0.357896 1:-85124131 MsG0180004977.01.T01:CDS
GTGGCAGGAATAATCAAATA+TGG 0.362119 1:-85123863 MsG0180004977.01.T01:CDS
TCTGAATCCATCGCTGCTTC+CGG 0.377026 1:+85123817 None:intergenic
TTTGGTCCTGGGTCTGGAGG+AGG 0.393596 1:+85123782 None:intergenic
TCAAATATGGTGAGAGGACT+TGG 0.397112 1:-85123850 MsG0180004977.01.T01:CDS
ACTTCGTAATTATGGCCATC+TGG 0.404255 1:-85124066 MsG0180004977.01.T01:CDS
ACCAAACTATGCTAAATTCA+TGG 0.409018 1:-85123765 MsG0180004977.01.T01:CDS
AATTTAGCATAGTTTGGTCC+TGG 0.419818 1:+85123770 None:intergenic
CCAAGATGCACAAGGAGAAA+TGG 0.430462 1:+85124040 None:intergenic
TAGGAACGGTGACATACTTA+AGG 0.432961 1:+85123891 None:intergenic
TTTATGTTGTTTATCGGTCA+TGG 0.437220 1:-85123925 MsG0180004977.01.T01:CDS
TTTCTCCTTGTGCATCTTGG+TGG 0.441469 1:-85124037 MsG0180004977.01.T01:CDS
GAGTAGGCAGTGATTGTGTC+AGG 0.469509 1:+85124013 None:intergenic
AGTATTAGGATCCAAAACTA+AGG 0.474378 1:+85123676 None:intergenic
TTGGTCCTGGGTCTGGAGGA+GGG 0.477210 1:+85123783 None:intergenic
TATCAACTACATGCTTCCTT+CGG 0.479055 1:+85124197 None:intergenic
ACCGTTCCTATTGTCGTGGC+AGG 0.487038 1:-85123878 MsG0180004977.01.T01:CDS
CTGCGGTCCGGAAGCAGCGA+TGG 0.489508 1:-85123824 MsG0180004977.01.T01:CDS
CTTCGTAATTATGGCCATCT+GGG 0.491238 1:-85124065 MsG0180004977.01.T01:CDS
CTTTACGTGATGGCGCTGTA+TGG 0.502909 1:-85123607 MsG0180004977.01.T01:CDS
ATTTAGCATAGTTTGGTCCT+GGG 0.515262 1:+85123771 None:intergenic
AGGACTTGGTCACTGCGGTC+CGG 0.530660 1:-85123836 MsG0180004977.01.T01:CDS
TGGGTTGCTACCGTTGCTCT+GGG 0.532615 1:-85124130 MsG0180004977.01.T01:CDS
TAGTTTGGTCCTGGGTCTGG+AGG 0.533953 1:+85123779 None:intergenic
GATGGCCATAATTACGAAGT+TGG 0.546121 1:+85124069 None:intergenic
AGGAACGGTGACATACTTAA+GGG 0.549631 1:+85123892 None:intergenic
AAGGAGAAATGGTGCCCAGA+TGG 0.549704 1:+85124051 None:intergenic
GGTGACATACTTAAGGGGAT+TGG 0.573721 1:+85123898 None:intergenic
TTGGTTGTCTTCAAGAGAGT+AGG 0.573804 1:+85123997 None:intergenic
GCATATGTCGTGGCCACAGT+TGG 0.576298 1:+85123978 None:intergenic
GATAGTCAAGCTTTACGTGA+TGG 0.584076 1:-85123617 MsG0180004977.01.T01:CDS
ATTCTCCCTCCTCCAGACCC+AGG 0.596606 1:-85123788 MsG0180004977.01.T01:CDS
GGAAAGAATTCCCAGAGCAA+CGG 0.617429 1:+85124120 None:intergenic
TGTCACCGTTCCTATTGTCG+TGG 0.631284 1:-85123882 MsG0180004977.01.T01:CDS
GAATAATCAAATATGGTGAG+AGG 0.632365 1:-85123856 MsG0180004977.01.T01:CDS
GTATTAGGATCCAAAACTAA+GGG 0.633824 1:+85123677 None:intergenic
TCCTGCCACGACAATAGGAA+CGG 0.646988 1:+85123877 None:intergenic
CTCTTGAAGACAACCAACTG+TGG 0.654311 1:-85123991 MsG0180004977.01.T01:CDS
CAGGACCACCAAGATGCACA+AGG 0.664529 1:+85124032 None:intergenic
GGAACGGTGACATACTTAAG+GGG 0.681590 1:+85123893 None:intergenic
AGTAATTCAAGCATATGTCG+TGG 0.691047 1:+85123968 None:intergenic
GTGAGAGGACTTGGTCACTG+CGG 0.730382 1:-85123841 MsG0180004977.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTGTATTTGGTATTT+TGG + Chr1:85124166-85124185 None:intergenic 15.0%
!! AGTTTCATTAATTTCATCTA+AGG + Chr1:85124106-85124125 None:intergenic 20.0%
! AAAGGCTAAAATTGTTGATA+TGG - Chr1:85123632-85123651 MsG0180004977.01.T01:CDS 25.0%
!! TTGCAGTTTATGTTGTTTAT+CGG - Chr1:85123875-85123894 MsG0180004977.01.T01:CDS 25.0%
!!! CTATCTGGAATTTTTGTATT+TGG + Chr1:85124174-85124193 None:intergenic 25.0%
ACCAAACTATGCTAAATTCA+TGG - Chr1:85124041-85124060 MsG0180004977.01.T01:CDS 30.0%
AGTATTAGGATCCAAAACTA+AGG + Chr1:85124133-85124152 None:intergenic 30.0%
CTTATGATCTTTAGTGTCTT+TGG + Chr1:85123710-85123729 None:intergenic 30.0%
GAATAATCAAATATGGTGAG+AGG - Chr1:85123950-85123969 MsG0180004977.01.T01:CDS 30.0%
GTATTAGGATCCAAAACTAA+GGG + Chr1:85124132-85124151 None:intergenic 30.0%
TACACTGCTAAAAAAGATGA+AGG - Chr1:85124069-85124088 MsG0180004977.01.T01:CDS 30.0%
TCCATGAATTTAGCATAGTT+TGG + Chr1:85124045-85124064 None:intergenic 30.0%
! TTTATGTTGTTTATCGGTCA+TGG - Chr1:85123881-85123900 MsG0180004977.01.T01:CDS 30.0%
! TTTTGGATCCTAATACTCAA+GGG - Chr1:85124136-85124155 MsG0180004977.01.T01:CDS 30.0%
AATTTAGCATAGTTTGGTCC+TGG + Chr1:85124039-85124058 None:intergenic 35.0%
AGACACCAACTTCGTAATTA+TGG - Chr1:85123732-85123751 MsG0180004977.01.T01:CDS 35.0%
AGTAATTCAAGCATATGTCG+TGG + Chr1:85123841-85123860 None:intergenic 35.0%
ATTTAGCATAGTTTGGTCCT+GGG + Chr1:85124038-85124057 None:intergenic 35.0%
CATAAACTGCAACTGCTAAT+TGG + Chr1:85123867-85123886 None:intergenic 35.0%
GTGGCAGGAATAATCAAATA+TGG - Chr1:85123943-85123962 MsG0180004977.01.T01:CDS 35.0%
TATCAACTACATGCTTCCTT+CGG + Chr1:85123612-85123631 None:intergenic 35.0%
! TGAAACTACTCCCTTAGTTT+TGG - Chr1:85124119-85124138 MsG0180004977.01.T01:CDS 35.0%
!! GGAAGCATGTAGTTGATAAA+AGG - Chr1:85123614-85123633 MsG0180004977.01.T01:CDS 35.0%
!! GTTTTGGATCCTAATACTCA+AGG - Chr1:85124135-85124154 MsG0180004977.01.T01:CDS 35.0%
ACTTCGTAATTATGGCCATC+TGG - Chr1:85123740-85123759 MsG0180004977.01.T01:CDS 40.0%
AGGAACGGTGACATACTTAA+GGG + Chr1:85123917-85123936 None:intergenic 40.0%
ATTATTCCTGCCACGACAAT+AGG + Chr1:85123937-85123956 None:intergenic 40.0%
CACTTATCTGTCAGCTGATT+GGG - Chr1:85123657-85123676 MsG0180004977.01.T01:CDS 40.0%
CTTCGTAATTATGGCCATCT+GGG - Chr1:85123741-85123760 MsG0180004977.01.T01:CDS 40.0%
GATAGTCAAGCTTTACGTGA+TGG - Chr1:85124189-85124208 MsG0180004977.01.T01:CDS 40.0%
GATGGCCATAATTACGAAGT+TGG + Chr1:85123740-85123759 None:intergenic 40.0%
TAGGAACGGTGACATACTTA+AGG + Chr1:85123918-85123937 None:intergenic 40.0%
TCAAATATGGTGAGAGGACT+TGG - Chr1:85123956-85123975 MsG0180004977.01.T01:CDS 40.0%
TCACGTAAAGCTTGACTATC+TGG + Chr1:85124189-85124208 None:intergenic 40.0%
TCACTTATCTGTCAGCTGAT+TGG - Chr1:85123656-85123675 MsG0180004977.01.T01:CDS 40.0%
TTGGTTGTCTTCAAGAGAGT+AGG + Chr1:85123812-85123831 None:intergenic 40.0%
! TTGGTTCTCCCTTGAGTATT+AGG + Chr1:85124147-85124166 None:intergenic 40.0%
!!! TCATCTTTTTGGGTAACCGA+AGG - Chr1:85123593-85123612 MsG0180004977.01.T01:CDS 40.0%
CCAAGATGCACAAGGAGAAA+TGG + Chr1:85123769-85123788 None:intergenic 45.0%
CCATTTCTCCTTGTGCATCT+TGG - Chr1:85123766-85123785 MsG0180004977.01.T01:CDS 45.0%
CTCTTGAAGACAACCAACTG+TGG - Chr1:85123815-85123834 MsG0180004977.01.T01:CDS 45.0%
GGAAAGAATTCCCAGAGCAA+CGG + Chr1:85123689-85123708 None:intergenic 45.0%
GGAACGGTGACATACTTAAG+GGG + Chr1:85123916-85123935 None:intergenic 45.0%
GGTGACATACTTAAGGGGAT+TGG + Chr1:85123911-85123930 None:intergenic 45.0%
TTTCTCCTTGTGCATCTTGG+TGG - Chr1:85123769-85123788 MsG0180004977.01.T01:CDS 45.0%
AAGGAGAAATGGTGCCCAGA+TGG + Chr1:85123758-85123777 None:intergenic 50.0%
GAGTAGGCAGTGATTGTGTC+AGG + Chr1:85123796-85123815 None:intergenic 50.0%
TCCTGCCACGACAATAGGAA+CGG + Chr1:85123932-85123951 None:intergenic 50.0%
TCTGAATCCATCGCTGCTTC+CGG + Chr1:85123992-85124011 None:intergenic 50.0%
TGTCACCGTTCCTATTGTCG+TGG - Chr1:85123924-85123943 MsG0180004977.01.T01:CDS 50.0%
! CTTTACGTGATGGCGCTGTA+TGG - Chr1:85124199-85124218 MsG0180004977.01.T01:CDS 50.0%
ACCGTTCCTATTGTCGTGGC+AGG - Chr1:85123928-85123947 MsG0180004977.01.T01:CDS 55.0%
CAGGACCACCAAGATGCACA+AGG + Chr1:85123777-85123796 None:intergenic 55.0%
GCATAGTTTGGTCCTGGGTC+TGG + Chr1:85124033-85124052 None:intergenic 55.0%
GCATATGTCGTGGCCACAGT+TGG + Chr1:85123831-85123850 None:intergenic 55.0%
TAGTTTGGTCCTGGGTCTGG+AGG + Chr1:85124030-85124049 None:intergenic 55.0%
TGGGTTGCTACCGTTGCTCT+GGG - Chr1:85123676-85123695 MsG0180004977.01.T01:CDS 55.0%
! GTGAGAGGACTTGGTCACTG+CGG - Chr1:85123965-85123984 MsG0180004977.01.T01:CDS 55.0%
! TTGGGTTGCTACCGTTGCTC+TGG - Chr1:85123675-85123694 MsG0180004977.01.T01:CDS 55.0%
ATTCTCCCTCCTCCAGACCC+AGG - Chr1:85124018-85124037 MsG0180004977.01.T01:CDS 60.0%
TTGGTCCTGGGTCTGGAGGA+GGG + Chr1:85124026-85124045 None:intergenic 60.0%
TTTGGTCCTGGGTCTGGAGG+AGG + Chr1:85124027-85124046 None:intergenic 60.0%
! AGGACTTGGTCACTGCGGTC+CGG - Chr1:85123970-85123989 MsG0180004977.01.T01:CDS 60.0%
CTGCGGTCCGGAAGCAGCGA+TGG - Chr1:85123982-85124001 MsG0180004977.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 85123586 85124242 85123586 ID=MsG0180004977.01;Name=MsG0180004977.01
Chr1 mRNA 85123586 85124242 85123586 ID=MsG0180004977.01.T01;Parent=MsG0180004977.01;Name=MsG0180004977.01.T01;_AED=0.47;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|218
Chr1 exon 85123586 85124242 85123586 ID=MsG0180004977.01.T01:exon:9557;Parent=MsG0180004977.01.T01
Chr1 CDS 85123586 85124242 85123586 ID=MsG0180004977.01.T01:cds;Parent=MsG0180004977.01.T01
Gene Sequence

>MsG0180004977.01.T01

ATGGTACTCATCTTTTTGGGTAACCGAAGGAAGCATGTAGTTGATAAAAGGCTAAAATTGTTGATATGGTTCACTTATCTGTCAGCTGATTGGGTTGCTACCGTTGCTCTGGGAATTCTTTCCAAAGACACTAAAGATCATAAGAAAGACACCAACTTCGTAATTATGGCCATCTGGGCACCATTTCTCCTTGTGCATCTTGGTGGTCCTGACACAATCACTGCCTACTCTCTTGAAGACAACCAACTGTGGCCACGACATATGCTTGAATTACTCTACCAATTAGCAGTTGCAGTTTATGTTGTTTATCGGTCATGGAACACCAATCCCCTTAAGTATGTCACCGTTCCTATTGTCGTGGCAGGAATAATCAAATATGGTGAGAGGACTTGGTCACTGCGGTCCGGAAGCAGCGATGGATTCAGAGAATCAATTCTCCCTCCTCCAGACCCAGGACCAAACTATGCTAAATTCATGGATGATTACACTGCTAAAAAAGATGAAGGATATCATGTTACCTTAGATGAAATTAATGAAACTACTCCCTTAGTTTTGGATCCTAATACTCAAGGGAGAACCAAAATACCAAATACAAAAATTCCAGATAGTCAAGCTTTACGTGATGGCGCTGTATGGCTCGAGTACAGTGTCAAATGA

Protein sequence

>MsG0180004977.01.T01

MVLIFLGNRRKHVVDKRLKLLIWFTYLSADWVATVALGILSKDTKDHKKDTNFVIMAIWAPFLLVHLGGPDTITAYSLEDNQLWPRHMLELLYQLAVAVYVVYRSWNTNPLKYVTVPIVVAGIIKYGERTWSLRSGSSDGFRESILPPPDPGPNYAKFMDDYTAKKDEGYHVTLDEINETTPLVLDPNTQGRTKIPNTKIPDSQALRDGAVWLEYSVK*