Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048896.01 | MsG0280009689.01 | -0.833192 | 6.142986e-56 | 7.022717e-53 |
| MsG0080049024.01 | MsG0280009689.01 | -0.806583 | 7.667573e-50 | 4.177072e-47 |
| MsG0180004322.01 | MsG0280009689.01 | -0.807797 | 4.240943e-50 | 2.384312e-47 |
| MsG0180004386.01 | MsG0280009689.01 | -0.801221 | 9.972455e-49 | 4.736508e-46 |
| MsG0180005833.01 | MsG0280009689.01 | -0.804162 | 2.465866e-49 | 1.262412e-46 |
| MsG0280007016.01 | MsG0280009689.01 | -0.838235 | 3.243262e-57 | 4.316701e-54 |
| MsG0280007017.01 | MsG0280009689.01 | -0.805391 | 1.365356e-49 | 7.214643e-47 |
| MsG0280007418.01 | MsG0280009689.01 | -0.803408 | 3.536936e-49 | 1.775988e-46 |
| MsG0280007653.01 | MsG0280009689.01 | -0.805310 | 1.419648e-49 | 7.486100e-47 |
| MsG0280009094.01 | MsG0280009689.01 | -0.806779 | 6.969124e-50 | 3.815795e-47 |
| MsG0280009689.01 | MsG0280011279.01 | -0.820187 | 7.829600e-53 | 6.152518e-50 |
| MsG0280009689.01 | MsG0280011281.01 | -0.804953 | 1.686523e-49 | 8.812574e-47 |
| MsG0280009689.01 | MsG0280011372.01 | -0.809226 | 2.100736e-50 | 1.226494e-47 |
| MsG0280009689.01 | MsG0380013501.01 | -0.800864 | 1.179894e-48 | 5.553754e-46 |
| MsG0280009689.01 | MsG0380014231.01 | -0.803355 | 3.627321e-49 | 1.818826e-46 |
| MsG0280009689.01 | MsG0380015369.01 | 0.804109 | 2.529615e-49 | 1.293290e-46 |
| MsG0280009689.01 | MsG0380015719.01 | -0.801140 | 1.036025e-48 | 4.910850e-46 |
| MsG0280009689.01 | MsG0380016231.01 | -0.805644 | 1.208451e-49 | 6.427316e-47 |
| MsG0280009689.01 | MsG0380016456.01 | -0.808067 | 3.716064e-50 | 2.104078e-47 |
| MsG0280009689.01 | MsG0380018035.01 | -0.805413 | 1.351052e-49 | 7.143245e-47 |
| MsG0280009689.01 | MsG0480018328.01 | -0.805427 | 1.341722e-49 | 7.096482e-47 |
| MsG0280009689.01 | MsG0480018629.01 | -0.804125 | 2.509398e-49 | 1.283505e-46 |
| MsG0280009689.01 | MsG0480018658.01 | -0.803914 | 2.776604e-49 | 1.412594e-46 |
| MsG0280009689.01 | MsG0480020928.01 | 0.806029 | 1.003291e-49 | 5.389058e-47 |
| MsG0280009689.01 | MsG0480022002.01 | -0.811700 | 6.141003e-51 | 3.827500e-48 |
| MsG0280009689.01 | MsG0480022411.01 | -0.810035 | 1.408171e-50 | 8.399456e-48 |
| MsG0280009689.01 | MsG0480022412.01 | -0.818316 | 2.089188e-52 | 1.558562e-49 |
| MsG0280009689.01 | MsG0480022458.01 | -0.822886 | 1.863250e-53 | 1.579540e-50 |
| MsG0280009689.01 | MsG0480023264.01 | -0.802924 | 4.453465e-49 | 2.208686e-46 |
| MsG0280009689.01 | MsG0480023298.01 | -0.822278 | 2.580895e-53 | 2.150382e-50 |
| MsG0280009689.01 | MsG0480023705.01 | -0.809278 | 2.047863e-50 | 1.197255e-47 |
| MsG0280009689.01 | MsG0480023993.01 | -0.805562 | 1.257588e-49 | 6.674262e-47 |
| MsG0280009689.01 | MsG0580024371.01 | -0.813691 | 2.251655e-51 | 1.480549e-48 |
| MsG0280009689.01 | MsG0580024386.01 | -0.806917 | 6.517370e-50 | 3.581262e-47 |
| MsG0280009689.01 | MsG0580025392.01 | -0.800570 | 1.354628e-48 | 6.328570e-46 |
| MsG0280009689.01 | MsG0580027276.01 | -0.801501 | 8.741197e-49 | 4.181171e-46 |
| MsG0280009689.01 | MsG0580028092.01 | -0.806613 | 7.555754e-50 | 4.119263e-47 |
| MsG0280009689.01 | MsG0580029820.01 | -0.807246 | 5.550121e-50 | 3.076044e-47 |
| MsG0280009689.01 | MsG0680030878.01 | -0.809853 | 1.540550e-50 | 9.145144e-48 |
| MsG0280009689.01 | MsG0680031204.01 | -0.811403 | 7.125622e-51 | 4.406870e-48 |
| MsG0280009689.01 | MsG0780038756.01 | -0.810540 | 1.095673e-50 | 6.622301e-48 |
| MsG0280009689.01 | MsG0780039704.01 | -0.813567 | 2.397907e-51 | 1.571425e-48 |
| MsG0280009689.01 | MsG0780039873.01 | -0.827384 | 1.611907e-54 | 1.553883e-51 |
| MsG0280009689.01 | MsG0780040158.01 | -0.809870 | 1.528057e-50 | 9.074968e-48 |
| MsG0280009689.01 | MsG0780040892.01 | -0.810216 | 1.286918e-50 | 7.711816e-48 |
| MsG0280009689.01 | MsG0880043515.01 | -0.803068 | 4.159284e-49 | 2.070205e-46 |
| MsG0280009689.01 | MsG0880044834.01 | -0.800353 | 1.499531e-48 | 6.967364e-46 |
| MsG0280009689.01 | MsG0880044968.01 | -0.801265 | 9.768050e-49 | 4.644571e-46 |
| MsG0280009689.01 | MsG0880045073.01 | -0.806757 | 7.042889e-50 | 3.853989e-47 |
| MsG0280009689.01 | MsG0880046759.01 | -0.820818 | 5.609258e-53 | 4.485466e-50 |
| MsG0280009689.01 | MsG0880047727.01 | -0.810461 | 1.139423e-50 | 6.872375e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTCAAACACCTTCTTAAC+AGG | 0.287299 | 2:-56343916 | None:intergenic |
| GGGTTACGGTGGATGGAAAT+CGG | 0.333557 | 2:+56343725 | None:intergenic |
| TGTTGTTGTGGTTGTGTTTG+TGG | 0.380790 | 2:+56343979 | MsG0280009689.01.T01:CDS |
| CGGTGGATGGAAATCGGGTT+TGG | 0.411477 | 2:+56343731 | None:intergenic |
| GGTTACGGTGGATGGAAATC+GGG | 0.428116 | 2:+56343726 | None:intergenic |
| TTCTACAACTCCTGTTAAGA+AGG | 0.469035 | 2:+56343906 | MsG0280009689.01.T01:CDS |
| AGAACAACCTCTTCAGAAAA+CGG | 0.487714 | 2:-56343887 | None:intergenic |
| GTGGGAGATCGTCGTGGTCG+TGG | 0.493390 | 2:+56343777 | MsG0280009689.01.T01:CDS |
| CATGCTCTTAGTTGTTGTTG+TGG | 0.518684 | 2:+56343967 | MsG0280009689.01.T01:CDS |
| CTGAATCAAGTGAGAGACGT+GGG | 0.621106 | 2:+56343759 | MsG0280009689.01.T01:CDS |
| TCTGAATCAAGTGAGAGACG+TGG | 0.710233 | 2:+56343758 | MsG0280009689.01.T01:CDS |
| AGAGACGTGGGAGATCGTCG+TGG | 0.745731 | 2:+56343771 | MsG0280009689.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTTCAGTGTCTTTCAACAA+TGG | + | Chr2:56343851-56343870 | MsG0280009689.01.T01:CDS | 30.0% |
| AGAACAACCTCTTCAGAAAA+CGG | - | Chr2:56343890-56343909 | None:intergenic | 35.0% | |
| CATTCAAACACCTTCTTAAC+AGG | - | Chr2:56343919-56343938 | None:intergenic | 35.0% | |
| TTCTACAACTCCTGTTAAGA+AGG | + | Chr2:56343906-56343925 | MsG0280009689.01.T01:CDS | 35.0% | |
| TGTTGTTGTGGTTGTGTTTG+TGG | + | Chr2:56343979-56343998 | MsG0280009689.01.T01:CDS | 40.0% | |
| ! | CATGCTCTTAGTTGTTGTTG+TGG | + | Chr2:56343967-56343986 | MsG0280009689.01.T01:CDS | 40.0% |
| !! | CAAAGAACCGTTTTCTGAAG+AGG | + | Chr2:56343880-56343899 | MsG0280009689.01.T01:CDS | 40.0% |
| CTGAATCAAGTGAGAGACGT+GGG | + | Chr2:56343759-56343778 | MsG0280009689.01.T01:CDS | 45.0% | |
| TCTGAATCAAGTGAGAGACG+TGG | + | Chr2:56343758-56343777 | MsG0280009689.01.T01:CDS | 45.0% | |
| AGAGACGTGGGAGATCGTCG+TGG | + | Chr2:56343771-56343790 | MsG0280009689.01.T01:CDS | 60.0% | |
| GTGGGAGATCGTCGTGGTCG+TGG | + | Chr2:56343777-56343796 | MsG0280009689.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 56343737 | 56344012 | 56343737 | ID=MsG0280009689.01;Name=MsG0280009689.01 |
| Chr2 | mRNA | 56343737 | 56344012 | 56343737 | ID=MsG0280009689.01.T01;Parent=MsG0280009689.01;Name=MsG0280009689.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|91 |
| Chr2 | exon | 56343737 | 56344012 | 56343737 | ID=MsG0280009689.01.T01:exon:18263;Parent=MsG0280009689.01.T01 |
| Chr2 | CDS | 56343737 | 56344012 | 56343737 | ID=MsG0280009689.01.T01:cds;Parent=MsG0280009689.01.T01 |
| Gene Sequence |
| Protein sequence |
