AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044237.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044237.01.T01 MTR_2g069350 53.333 60 28 0 9 68 306 365 9.75e-18 75.1
MsG0880044237.01.T01 MTR_2g028830 59.184 49 20 0 20 68 270 318 1.45e-15 68.9
MsG0880044237.01.T01 MTR_4g083540 55.102 49 22 0 20 68 244 292 5.46e-15 67.0
MsG0880044237.01.T01 MTR_5g013690 46.809 47 25 0 22 68 225 271 7.94e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044237.01.T01 AT1G79110 56.061 66 22 1 3 68 273 331 3.74e-18 76.3
MsG0880044237.01.T01 AT1G79110 56.061 66 22 1 3 68 295 353 3.87e-18 76.3
MsG0880044237.01.T01 AT1G79110 56.061 66 22 1 3 68 298 356 3.90e-18 76.3
MsG0880044237.01.T01 AT3G12920 62.500 48 18 0 21 68 286 333 6.15e-17 72.8
MsG0880044237.01.T01 AT4G19700 55.102 49 20 1 22 68 254 302 2.72e-14 65.5
MsG0880044237.01.T01 AT5G45100 53.061 49 21 1 22 68 217 265 3.44e-13 62.0
MsG0880044237.01.T01 AT5G45100 53.061 49 21 1 22 68 244 292 3.78e-13 62.4

Find 14 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTTCAATGTGTGGGGCTA+TGG 0.402214 8:+38219688 MsG0880044237.01.T01:CDS
AGTAATGACGGCGCAGGATA+AGG 0.404721 8:+38219559 None:intergenic
TATGTTTGTGTTCAATGTGT+GGG 0.464104 8:+38219681 MsG0880044237.01.T01:CDS
CACAAACATAAGTGTCTACA+AGG 0.505076 8:-38219668 None:intergenic
GATTTAACAGAATCACATAT+AGG 0.518774 8:-38219722 None:intergenic
AAGGATGAAGGTACTAGTAG+TGG 0.520250 8:+38219578 MsG0880044237.01.T01:CDS
ATAGATTATGCAGAAACTGT+GGG 0.549558 8:+38219618 MsG0880044237.01.T01:CDS
TTATGTTTGTGTTCAATGTG+TGG 0.554213 8:+38219680 MsG0880044237.01.T01:CDS
AATAGATTATGCAGAAACTG+TGG 0.580193 8:+38219617 MsG0880044237.01.T01:CDS
TTATGCAGAAACTGTGGGGA+AGG 0.589265 8:+38219623 MsG0880044237.01.T01:CDS
ATGTTTGTGTTCAATGTGTG+GGG 0.595264 8:+38219682 MsG0880044237.01.T01:CDS
ACGGCGCAGGATAAGGATGA+AGG 0.596413 8:+38219566 MsG0880044237.01.T01:CDS
TACGGTAGTAATGACGGCGC+AGG 0.633033 8:+38219553 None:intergenic
TAGATTATGCAGAAACTGTG+GGG 0.699966 8:+38219619 MsG0880044237.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTTAACAGAATCACATAT+AGG - Chr8:38219725-38219744 None:intergenic 25.0%
AATAGATTATGCAGAAACTG+TGG + Chr8:38219617-38219636 MsG0880044237.01.T01:CDS 30.0%
ATAGATTATGCAGAAACTGT+GGG + Chr8:38219618-38219637 MsG0880044237.01.T01:CDS 30.0%
TATGTTTGTGTTCAATGTGT+GGG + Chr8:38219681-38219700 MsG0880044237.01.T01:CDS 30.0%
TTATGTTTGTGTTCAATGTG+TGG + Chr8:38219680-38219699 MsG0880044237.01.T01:CDS 30.0%
ATGTTTGTGTTCAATGTGTG+GGG + Chr8:38219682-38219701 MsG0880044237.01.T01:CDS 35.0%
CACAAACATAAGTGTCTACA+AGG - Chr8:38219671-38219690 None:intergenic 35.0%
TAGATTATGCAGAAACTGTG+GGG + Chr8:38219619-38219638 MsG0880044237.01.T01:CDS 35.0%
!! AAGGATGAAGGTACTAGTAG+TGG + Chr8:38219578-38219597 MsG0880044237.01.T01:CDS 40.0%
TTATGCAGAAACTGTGGGGA+AGG + Chr8:38219623-38219642 MsG0880044237.01.T01:CDS 45.0%
GTGTTCAATGTGTGGGGCTA+TGG + Chr8:38219688-38219707 MsG0880044237.01.T01:CDS 50.0%
ACGGCGCAGGATAAGGATGA+AGG + Chr8:38219566-38219585 MsG0880044237.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 38219563 38219772 38219563 ID=MsG0880044237.01;Name=MsG0880044237.01
Chr8 mRNA 38219563 38219772 38219563 ID=MsG0880044237.01.T01;Parent=MsG0880044237.01;Name=MsG0880044237.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|69
Chr8 exon 38219563 38219772 38219563 ID=MsG0880044237.01.T01:exon:16198;Parent=MsG0880044237.01.T01
Chr8 CDS 38219563 38219772 38219563 ID=MsG0880044237.01.T01:cds;Parent=MsG0880044237.01.T01
Gene Sequence

>MsG0880044237.01.T01

ATGACGGCGCAGGATAAGGATGAAGGTACTAGTAGTGGTAGAGTGATGAATAACAATAGATTATGCAGAAACTGTGGGGAAGGTGAATCTTGTGTATTGATCATGCCTTGTAGACACTTATGTTTGTGTTCAATGTGTGGGGCTATGGTTCTTACTTGTCCTATATGTGATTCTGTTAAATCTGCTACTCTTAATGTTAATCTTGATTAA

Protein sequence

>MsG0880044237.01.T01

MTAQDKDEGTSSGRVMNNNRLCRNCGEGESCVLIMPCRHLCLCSMCGAMVLTCPICDSVKSATLNVNLD*