Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011286.01.T01 | GAU10972.1 | 66.279 | 86 | 3 | 1 | 2 | 87 | 109 | 168 | 7.90E-27 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011286.01.T01 | Q8S3D1 | 47.674 | 86 | 18 | 2 | 2 | 87 | 285 | 343 | 3.67E-14 | 69.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011286.01.T01 | A0A2Z6LJG6 | 66.279 | 86 | 3 | 1 | 2 | 87 | 109 | 168 | 3.77e-27 | 108 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049014.01 | MsG0280011286.01 | 0.830550 | 2.756950e-55 | 2.914023e-52 |
| MsG0080049058.01 | MsG0280011286.01 | 0.834373 | 3.111098e-56 | 3.685059e-53 |
| MsG0180000567.01 | MsG0280011286.01 | 0.804017 | 2.642712e-49 | 1.347981e-46 |
| MsG0180001006.01 | MsG0280011286.01 | 0.804471 | 2.126519e-49 | 1.097341e-46 |
| MsG0180001471.01 | MsG0280011286.01 | 0.815252 | 1.017070e-51 | 6.975934e-49 |
| MsG0180001565.01 | MsG0280011286.01 | 0.804157 | 2.471826e-49 | 1.265318e-46 |
| MsG0180002894.01 | MsG0280011286.01 | 0.815400 | 9.425597e-52 | 6.491692e-49 |
| MsG0180002952.01 | MsG0280011286.01 | 0.815400 | 9.425597e-52 | 6.491692e-49 |
| MsG0180003690.01 | MsG0280011286.01 | 0.802088 | 6.623423e-49 | 3.215659e-46 |
| MsG0180004060.01 | MsG0280011286.01 | 0.801947 | 7.079544e-49 | 3.424720e-46 |
| MsG0180004114.01 | MsG0280011286.01 | 0.813998 | 1.926863e-51 | 1.277549e-48 |
| MsG0180004388.01 | MsG0280011286.01 | 0.831467 | 1.642211e-55 | 1.783681e-52 |
| MsG0180005050.01 | MsG0280011286.01 | 0.815336 | 9.740772e-52 | 6.697025e-49 |
| MsG0180005285.01 | MsG0280011286.01 | 0.803338 | 3.655757e-49 | 1.832337e-46 |
| MsG0180005860.01 | MsG0280011286.01 | 0.826162 | 3.155153e-54 | 2.935856e-51 |
| MsG0280006299.01 | MsG0280011286.01 | 0.806262 | 8.961418e-50 | 4.842404e-47 |
| MsG0280006375.01 | MsG0280011286.01 | 0.827437 | 1.565511e-54 | 1.511344e-51 |
| MsG0280010614.01 | MsG0280011286.01 | 0.831184 | 1.927868e-55 | 2.076223e-52 |
| MsG0280010824.01 | MsG0280011286.01 | 0.800813 | 1.208457e-48 | 5.680456e-46 |
| MsG0280011282.01 | MsG0280011286.01 | 0.806838 | 6.771876e-50 | 3.713608e-47 |
| MsG0280011285.01 | MsG0280011286.01 | 0.889883 | 1.624638e-73 | 1.334905e-69 |
| MsG0280011286.01 | MsG0280011293.01 | 0.809701 | 1.661304e-50 | 9.822578e-48 |
| MsG0280011286.01 | MsG0380012403.01 | 0.818001 | 2.461270e-52 | 1.820327e-49 |
| MsG0280011286.01 | MsG0380013834.01 | 0.823375 | 1.432854e-53 | 1.231421e-50 |
| MsG0280011286.01 | MsG0380014573.01 | 0.821406 | 4.105746e-53 | 3.337278e-50 |
| MsG0280011286.01 | MsG0380015449.01 | 0.802417 | 5.666584e-49 | 2.774421e-46 |
| MsG0280011286.01 | MsG0380015455.01 | 0.803974 | 2.697749e-49 | 1.374540e-46 |
| MsG0280011286.01 | MsG0380016234.01 | 0.807719 | 4.406527e-50 | 2.472357e-47 |
| MsG0280011286.01 | MsG0380016236.01 | 0.817400 | 3.362937e-52 | 2.446557e-49 |
| MsG0280011286.01 | MsG0380016264.01 | 0.831201 | 1.908728e-55 | 2.056708e-52 |
| MsG0280011286.01 | MsG0380016553.01 | 0.821336 | 4.262539e-53 | 3.458038e-50 |
| MsG0280011286.01 | MsG0380016859.01 | 0.800442 | 1.438798e-48 | 6.700043e-46 |
| MsG0280011286.01 | MsG0380017680.01 | 0.806961 | 6.380078e-50 | 3.509727e-47 |
| MsG0280011286.01 | MsG0380018009.01 | 0.836252 | 1.043626e-56 | 1.308075e-53 |
| MsG0280011286.01 | MsG0480018163.01 | 0.830619 | 2.651389e-55 | 2.808295e-52 |
| MsG0280011286.01 | MsG0480018576.01 | 0.839273 | 1.748623e-57 | 2.402540e-54 |
| MsG0280011286.01 | MsG0480018586.01 | 0.800448 | 1.434554e-48 | 6.681225e-46 |
| MsG0280011286.01 | MsG0480018640.01 | 0.803847 | 2.867051e-49 | 1.456058e-46 |
| MsG0280011286.01 | MsG0480018970.01 | 0.820759 | 5.787009e-53 | 4.620030e-50 |
| MsG0280011286.01 | MsG0480020559.01 | 0.811831 | 5.750338e-51 | 3.596623e-48 |
| MsG0280011286.01 | MsG0480020634.01 | 0.801703 | 7.945952e-49 | 3.820051e-46 |
| MsG0280011286.01 | MsG0480020674.01 | 0.806787 | 6.943374e-50 | 3.802422e-47 |
| MsG0280011286.01 | MsG0480020886.01 | 0.809705 | 1.658200e-50 | 9.805104e-48 |
| MsG0280011286.01 | MsG0480022480.01 | 0.812682 | 3.748997e-51 | 2.399068e-48 |
| MsG0280011286.01 | MsG0480022482.01 | 0.810165 | 1.319886e-50 | 7.899270e-48 |
| MsG0280011286.01 | MsG0480022551.01 | 0.811360 | 7.280071e-51 | 4.496895e-48 |
| MsG0280011286.01 | MsG0480023410.01 | 0.879163 | 1.559639e-69 | 8.367616e-66 |
| MsG0280011286.01 | MsG0480023707.01 | 0.837084 | 6.402655e-57 | 8.226364e-54 |
| MsG0280011286.01 | MsG0480023835.01 | 0.849850 | 2.481212e-60 | 4.767371e-57 |
| MsG0280011286.01 | MsG0580024073.01 | 0.802265 | 6.090084e-49 | 2.970108e-46 |
| MsG0280011286.01 | MsG0580024082.01 | 0.804078 | 2.567592e-49 | 1.311696e-46 |
| MsG0280011286.01 | MsG0580024393.01 | 0.821925 | 3.114773e-53 | 2.569221e-50 |
| MsG0280011286.01 | MsG0580026023.01 | 0.804953 | 1.686723e-49 | 8.813569e-47 |
| MsG0280011286.01 | MsG0580026070.01 | 0.817299 | 3.543223e-52 | 2.570523e-49 |
| MsG0280011286.01 | MsG0580027049.01 | 0.833453 | 5.286656e-56 | 6.091095e-53 |
| MsG0280011286.01 | MsG0580027208.01 | 0.813055 | 3.106207e-51 | 2.007417e-48 |
| MsG0280011286.01 | MsG0580028307.01 | 0.816588 | 5.118897e-52 | 3.642022e-49 |
| MsG0280011286.01 | MsG0580029155.01 | 0.807063 | 6.070732e-50 | 3.348406e-47 |
| MsG0280011286.01 | MsG0580029278.01 | 0.802556 | 5.304049e-49 | 2.605915e-46 |
| MsG0280011286.01 | MsG0580029960.01 | 0.805873 | 1.081635e-49 | 5.786921e-47 |
| MsG0280011286.01 | MsG0680031262.01 | 0.812016 | 5.240930e-51 | 3.294374e-48 |
| MsG0280011286.01 | MsG0680035573.01 | 0.823076 | 1.683013e-53 | 1.434495e-50 |
| MsG0280011286.01 | MsG0780038495.01 | 0.829014 | 6.524780e-55 | 6.594687e-52 |
| MsG0280011286.01 | MsG0780039249.01 | 0.815721 | 7.994706e-52 | 5.555191e-49 |
| MsG0280011286.01 | MsG0780039252.01 | 0.841024 | 6.106240e-58 | 8.856476e-55 |
| MsG0280011286.01 | MsG0780040488.01 | 0.822627 | 2.141007e-53 | 1.801867e-50 |
| MsG0280011286.01 | MsG0780040601.01 | 0.806676 | 7.327948e-50 | 4.001485e-47 |
| MsG0280011286.01 | MsG0780040604.01 | 0.807523 | 4.847843e-50 | 2.706191e-47 |
| MsG0280011286.01 | MsG0780040669.01 | 0.814342 | 1.617618e-51 | 1.082621e-48 |
| MsG0280011286.01 | MsG0780040704.01 | 0.801068 | 1.072152e-48 | 5.072748e-46 |
| MsG0280011286.01 | MsG0780040723.01 | 0.858563 | 7.559124e-63 | 1.937176e-59 |
| MsG0280011286.01 | MsG0780041429.01 | 0.806891 | 6.598953e-50 | 3.623628e-47 |
| MsG0280011286.01 | MsG0880042158.01 | 0.817755 | 2.797337e-52 | 2.054999e-49 |
| MsG0280011286.01 | MsG0880042232.01 | 0.817687 | 2.897374e-52 | 2.124481e-49 |
| MsG0280011286.01 | MsG0880042543.01 | 0.850126 | 2.077203e-60 | 4.027263e-57 |
| MsG0280011286.01 | MsG0880042967.01 | 0.801225 | 9.955334e-49 | 4.728829e-46 |
| MsG0280011286.01 | MsG0880043299.01 | 0.830155 | 3.444147e-55 | 3.599176e-52 |
| MsG0280011286.01 | MsG0880044486.01 | 0.802867 | 4.575166e-49 | 2.265804e-46 |
| MsG0280011286.01 | MsG0880045937.01 | 0.804835 | 1.785396e-49 | 9.300738e-47 |
| MsG0280011286.01 | MsG0880046012.01 | 0.812231 | 4.704684e-51 | 2.974783e-48 |
| MsG0280011286.01 | MsG0880046020.01 | 0.817886 | 2.612713e-52 | 1.926228e-49 |
| MsG0280011286.01 | MsG0880046479.01 | 0.836146 | 1.110258e-56 | 1.386979e-53 |
| MsG0280006976.01 | MsG0280011286.01 | 0.833734 | 4.498387e-56 | 5.226371e-53 |
| MsG0280007089.01 | MsG0280011286.01 | 0.815835 | 7.542783e-52 | 5.256971e-49 |
| MsG0280008384.01 | MsG0280011286.01 | 0.818395 | 2.004477e-52 | 1.498744e-49 |
| MsG0280009514.01 | MsG0280011286.01 | 0.805796 | 1.123079e-49 | 5.996625e-47 |
| MsG0280009748.01 | MsG0280011286.01 | 0.808268 | 3.366979e-50 | 1.916666e-47 |
| MsG0280009835.01 | MsG0280011286.01 | 0.808249 | 3.398341e-50 | 1.933565e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011286.01.T01 | MTR_2g101520 | 67.442 | 86 | 2 | 1 | 2 | 87 | 211 | 270 | 1.84e-30 | 110 |
| MsG0280011286.01.T01 | MTR_6g488100 | 45.455 | 88 | 20 | 2 | 2 | 89 | 209 | 268 | 1.38e-14 | 67.0 |
| MsG0280011286.01.T01 | MTR_6g488100 | 45.349 | 86 | 19 | 2 | 2 | 87 | 209 | 266 | 5.27e-14 | 65.9 |
| MsG0280011286.01.T01 | MTR_8g103065 | 46.591 | 88 | 18 | 3 | 2 | 87 | 218 | 278 | 1.36e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011286.01.T01 | AT4G29100 | 48.837 | 86 | 17 | 2 | 2 | 87 | 285 | 343 | 1.47e-15 | 70.5 |
| MsG0280011286.01.T01 | AT4G29100 | 47.674 | 86 | 18 | 2 | 2 | 87 | 285 | 343 | 3.74e-15 | 69.3 |
Find 17 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCGGTCAGGTATGCTTTAT+TGG | 0.204316 | 2:+81961084 | MsG0280011286.01.T01:CDS |
| ATAAATAAATAAGAATAATA+TGG | 0.289543 | 2:-81960660 | None:intergenic |
| CATACCTGACCGGGGTCTTC+AGG | 0.315826 | 2:-81961075 | None:intergenic |
| ATTTGATTCTAATTAATTGA+TGG | 0.321394 | 2:-81961109 | None:intergenic |
| GGCACTCAGCACCCCTTACT+TGG | 0.395598 | 2:+81960821 | MsG0280011286.01.T01:intron |
| GATGCTGCAGTGTCCAAGTA+AGG | 0.413970 | 2:-81960834 | None:intergenic |
| TAGAAGCCATTGGATACATC+AGG | 0.435008 | 2:+81960607 | MsG0280011286.01.T01:CDS |
| CCAATAAAGCATACCTGACC+GGG | 0.469066 | 2:-81961084 | None:intergenic |
| TCTGTATTATTAGAAGCCAT+TGG | 0.473376 | 2:+81960597 | MsG0280011286.01.T01:CDS |
| TCCAATAAAGCATACCTGAC+CGG | 0.484473 | 2:-81961085 | None:intergenic |
| ATTTCCTGAAGACCCCGGTC+AGG | 0.529122 | 2:+81961071 | MsG0280011286.01.T01:CDS |
| GTTTCTACAGAGTCAAATTG+AGG | 0.544344 | 2:+81960629 | MsG0280011286.01.T01:CDS |
| TAGAAACCTGATGTATCCAA+TGG | 0.592817 | 2:-81960613 | None:intergenic |
| ATGCTGCAGTGTCCAAGTAA+GGG | 0.597172 | 2:-81960833 | None:intergenic |
| TCTGTATTTCCTGAAGACCC+CGG | 0.639899 | 2:+81961066 | MsG0280011286.01.T01:CDS |
| TGCTGCAGTGTCCAAGTAAG+GGG | 0.663325 | 2:-81960832 | None:intergenic |
| CAATAAAGCATACCTGACCG+GGG | 0.742945 | 2:-81961083 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAAAATAAAATTAAAAA+AGG | - | Chr2:81961014-81961033 | None:intergenic | 0.0% |
| !! | AAAATAAAATTAAAAAAGGA+AGG | - | Chr2:81961010-81961029 | None:intergenic | 10.0% |
| !! | AATATAGTATTATTATTGAA+GGG | + | Chr2:81960974-81960993 | MsG0280011286.01.T01:intron | 10.0% |
| !!! | TTTTATAAAAAACTATTAAC+AGG | + | Chr2:81961026-81961045 | MsG0280011286.01.T01:intron | 10.0% |
| !! | GAATATAGTATTATTATTGA+AGG | + | Chr2:81960973-81960992 | MsG0280011286.01.T01:intron | 15.0% |
| !! | AAACATTCTTTCTTTAGTTA+TGG | + | Chr2:81960764-81960783 | MsG0280011286.01.T01:intron | 20.0% |
| !! | AACATTCTTTCTTTAGTTAT+GGG | + | Chr2:81960765-81960784 | MsG0280011286.01.T01:intron | 20.0% |
| ! | GAAATTGAATAGGACAAAAA+TGG | + | Chr2:81960920-81960939 | MsG0280011286.01.T01:intron | 25.0% |
| !!! | ACTTTTCTCTGAAATTGAAT+AGG | + | Chr2:81960910-81960929 | MsG0280011286.01.T01:intron | 25.0% |
| TCAGAGAAAAGTGAAACATT+GGG | - | Chr2:81960902-81960921 | None:intergenic | 30.0% | |
| TCTGTATTATTAGAAGCCAT+TGG | + | Chr2:81960597-81960616 | MsG0280011286.01.T01:CDS | 30.0% | |
| TTCAGAGAAAAGTGAAACAT+TGG | - | Chr2:81960903-81960922 | None:intergenic | 30.0% | |
| CAGCATCAAAAAACATGATG+AGG | + | Chr2:81960850-81960869 | MsG0280011286.01.T01:CDS | 35.0% | |
| GTTTCTACAGAGTCAAATTG+AGG | + | Chr2:81960629-81960648 | MsG0280011286.01.T01:CDS | 35.0% | |
| TAGAAACCTGATGTATCCAA+TGG | - | Chr2:81960616-81960635 | None:intergenic | 35.0% | |
| ! | TTATTCCTCTCTTTTGTTGC+AGG | + | Chr2:81960800-81960819 | MsG0280011286.01.T01:intron | 35.0% |
| TAGAAGCCATTGGATACATC+AGG | + | Chr2:81960607-81960626 | MsG0280011286.01.T01:CDS | 40.0% | |
| TCCAATAAAGCATACCTGAC+CGG | - | Chr2:81961088-81961107 | None:intergenic | 40.0% | |
| ATGCTGCAGTGTCCAAGTAA+GGG | - | Chr2:81960836-81960855 | None:intergenic | 45.0% | |
| CAATAAAGCATACCTGACCG+GGG | - | Chr2:81961086-81961105 | None:intergenic | 45.0% | |
| CCAATAAAGCATACCTGACC+GGG | - | Chr2:81961087-81961106 | None:intergenic | 45.0% | |
| TCTGTATTTCCTGAAGACCC+CGG | + | Chr2:81961066-81961085 | MsG0280011286.01.T01:CDS | 45.0% | |
| !! | ATAAATAAATAAGAATAATA+TGG | - | Chr2:81960663-81960682 | None:intergenic | 5.0% |
| CCCGGTCAGGTATGCTTTAT+TGG | + | Chr2:81961084-81961103 | MsG0280011286.01.T01:CDS | 50.0% | |
| GAGTGCCTGCAACAAAAGAG+AGG | - | Chr2:81960808-81960827 | None:intergenic | 50.0% | |
| GATGCTGCAGTGTCCAAGTA+AGG | - | Chr2:81960837-81960856 | None:intergenic | 50.0% | |
| TGCTGCAGTGTCCAAGTAAG+GGG | - | Chr2:81960835-81960854 | None:intergenic | 50.0% | |
| ATTTCCTGAAGACCCCGGTC+AGG | + | Chr2:81961071-81961090 | MsG0280011286.01.T01:CDS | 55.0% | |
| CATACCTGACCGGGGTCTTC+AGG | - | Chr2:81961078-81961097 | None:intergenic | 60.0% | |
| GGCACTCAGCACCCCTTACT+TGG | + | Chr2:81960821-81960840 | MsG0280011286.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 81960579 | 81961119 | 81960579 | ID=MsG0280011286.01;Name=MsG0280011286.01 |
| Chr2 | mRNA | 81960579 | 81961119 | 81960579 | ID=MsG0280011286.01.T01;Parent=MsG0280011286.01;Name=MsG0280011286.01.T01;_AED=0.48;_eAED=0.53;_QI=0|0.5|0.33|1|0.5|0.66|3|0|94 |
| Chr2 | exon | 81960579 | 81960728 | 81960579 | ID=MsG0280011286.01.T01:exon:15859;Parent=MsG0280011286.01.T01 |
| Chr2 | exon | 81960822 | 81960884 | 81960822 | ID=MsG0280011286.01.T01:exon:15860;Parent=MsG0280011286.01.T01 |
| Chr2 | exon | 81961048 | 81961119 | 81961048 | ID=MsG0280011286.01.T01:exon:15861;Parent=MsG0280011286.01.T01 |
| Chr2 | CDS | 81960579 | 81960728 | 81960579 | ID=MsG0280011286.01.T01:cds;Parent=MsG0280011286.01.T01 |
| Chr2 | CDS | 81960822 | 81960884 | 81960822 | ID=MsG0280011286.01.T01:cds;Parent=MsG0280011286.01.T01 |
| Chr2 | CDS | 81961048 | 81961119 | 81961048 | ID=MsG0280011286.01.T01:cds;Parent=MsG0280011286.01.T01 |
| Gene Sequence |
| Protein sequence |
