AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023162.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023162.01.T01 MTR_8g088370 91.304 69 6 0 1 69 1266 1334 9.59e-37 130
MsG0480023162.01.T01 MTR_2g030160 71.014 69 20 0 1 69 731 799 8.39e-29 107
MsG0480023162.01.T01 MTR_2g030630 45.312 64 33 1 1 64 1221 1282 2.08e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023162.01.T01 AT4G02510 84.375 64 10 0 1 64 1381 1444 8.70e-31 113
MsG0480023162.01.T01 AT4G02510 84.375 64 10 0 1 64 1381 1444 8.70e-31 113
MsG0480023162.01.T01 AT4G02510 84.375 64 10 0 1 64 1381 1444 8.70e-31 113
MsG0480023162.01.T01 AT4G02510 84.375 64 10 0 1 64 1381 1444 8.70e-31 113
MsG0480023162.01.T01 AT3G16620 45.161 62 34 0 3 64 972 1033 3.20e-14 66.2
MsG0480023162.01.T01 AT2G16640 45.161 62 34 0 3 64 1090 1151 3.82e-14 65.9
MsG0480023162.01.T01 AT2G16640 45.161 62 34 0 3 64 1090 1151 3.82e-14 65.9
MsG0480023162.01.T01 AT2G16640 45.161 62 34 0 3 64 1090 1151 3.82e-14 65.9

Find 26 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAATCTGTGACTGGAAAT+TGG 0.308064 4:-81623982 None:intergenic
TCTCAAGGTGATTCTGCTTA+TGG 0.363951 4:+81623852 MsG0480023162.01.T01:CDS
GAAGACTGATCCTGCCCAAT+TGG 0.401018 4:-81623912 None:intergenic
GTCTTCATTGAGTTTCTCTC+TGG 0.403945 4:+81623929 MsG0480023162.01.T01:CDS
TTGGTATTAGTGGGGAGCAC+TGG 0.434068 4:+81623819 MsG0480023162.01.T01:CDS
TTCCAGTCACAGATTTCTCT+TGG 0.439411 4:+81623987 MsG0480023162.01.T01:CDS
AGGGAAACGATTGGTATTAG+TGG 0.494090 4:+81623809 None:intergenic
GGGAAACGATTGGTATTAGT+GGG 0.496473 4:+81623810 None:intergenic
TGGTATTAGTGGGGAGCACT+GGG 0.509804 4:+81623820 MsG0480023162.01.T01:CDS
ACTGGGACCGTGCGGTCTCA+AGG 0.510375 4:+81623837 MsG0480023162.01.T01:CDS
TTCTCTCTGGTGCAGTGGAG+AGG 0.514240 4:+81623942 MsG0480023162.01.T01:CDS
CGCCAAGAGAAATCTGTGAC+TGG 0.527890 4:-81623989 None:intergenic
TGAGTTTCTCTCTGGTGCAG+TGG 0.529140 4:+81623937 MsG0480023162.01.T01:CDS
CTCTTGGCGAAGCTATAAGA+TGG 0.536070 4:+81624003 MsG0480023162.01.T01:CDS
CTATAAGATGGCGGTTCGTG+CGG 0.537307 4:+81624015 MsG0480023162.01.T01:CDS
TCTAAGCCTCACTTCAACAT+TGG 0.541559 4:-81623877 None:intergenic
GGATTGAACAACAAGCTTAG+TGG 0.560199 4:+81624036 MsG0480023162.01.T01:CDS
TGGAGAGGTGATTTAGCCGT+GGG 0.589597 4:+81623957 MsG0480023162.01.T01:CDS
GTGGAGAGGTGATTTAGCCG+TGG 0.611173 4:+81623956 MsG0480023162.01.T01:CDS
TGTTGAAGTGAGGCTTAGAG+AGG 0.624868 4:+81623881 MsG0480023162.01.T01:CDS
TGACTGGAAATTGGCTCCCA+CGG 0.635705 4:-81623973 None:intergenic
ATGGAGCCAATGTTGAAGTG+AGG 0.641534 4:+81623871 MsG0480023162.01.T01:CDS
TGGGGAGCACTGGGACCGTG+CGG 0.658055 4:+81623829 MsG0480023162.01.T01:CDS
AGAATCACCTTGAGACCGCA+CGG 0.671150 4:-81623844 None:intergenic
TTGGCGAAGCTATAAGATGG+CGG 0.700030 4:+81624006 MsG0480023162.01.T01:CDS
GGAAACGATTGGTATTAGTG+GGG 0.717259 4:+81623811 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAATCTGTGACTGGAAAT+TGG - Chr4:81623985-81624004 None:intergenic 35.0%
GTCTTCATTGAGTTTCTCTC+TGG + Chr4:81623929-81623948 MsG0480023162.01.T01:CDS 40.0%
TCTAAGCCTCACTTCAACAT+TGG - Chr4:81623880-81623899 None:intergenic 40.0%
TCTCAAGGTGATTCTGCTTA+TGG + Chr4:81623852-81623871 MsG0480023162.01.T01:CDS 40.0%
TTCCAGTCACAGATTTCTCT+TGG + Chr4:81623987-81624006 MsG0480023162.01.T01:CDS 40.0%
! AGAGAGGCAGATTTTCCAAT+TGG + Chr4:81623897-81623916 MsG0480023162.01.T01:CDS 40.0%
! GAGAGGCAGATTTTCCAATT+GGG + Chr4:81623898-81623917 MsG0480023162.01.T01:CDS 40.0%
ATGGAGCCAATGTTGAAGTG+AGG + Chr4:81623871-81623890 MsG0480023162.01.T01:CDS 45.0%
CTCTTGGCGAAGCTATAAGA+TGG + Chr4:81624003-81624022 MsG0480023162.01.T01:CDS 45.0%
TGTTGAAGTGAGGCTTAGAG+AGG + Chr4:81623881-81623900 MsG0480023162.01.T01:CDS 45.0%
TTGGCGAAGCTATAAGATGG+CGG + Chr4:81624006-81624025 MsG0480023162.01.T01:CDS 45.0%
AGAATCACCTTGAGACCGCA+CGG - Chr4:81623847-81623866 None:intergenic 50.0%
CGCCAAGAGAAATCTGTGAC+TGG - Chr4:81623992-81624011 None:intergenic 50.0%
GAAGACTGATCCTGCCCAAT+TGG - Chr4:81623915-81623934 None:intergenic 50.0%
TGAGTTTCTCTCTGGTGCAG+TGG + Chr4:81623937-81623956 MsG0480023162.01.T01:CDS 50.0%
TGGAGAGGTGATTTAGCCGT+GGG + Chr4:81623957-81623976 MsG0480023162.01.T01:CDS 50.0%
! CTATAAGATGGCGGTTCGTG+CGG + Chr4:81624015-81624034 MsG0480023162.01.T01:CDS 50.0%
! GGCAGATTTTCCAATTGGGC+AGG + Chr4:81623902-81623921 MsG0480023162.01.T01:CDS 50.0%
! TGACTGGAAATTGGCTCCCA+CGG - Chr4:81623976-81623995 None:intergenic 50.0%
! TTGGTATTAGTGGGGAGCAC+TGG + Chr4:81623819-81623838 MsG0480023162.01.T01:CDS 50.0%
!! TGGTATTAGTGGGGAGCACT+GGG + Chr4:81623820-81623839 MsG0480023162.01.T01:CDS 50.0%
GTGGAGAGGTGATTTAGCCG+TGG + Chr4:81623956-81623975 MsG0480023162.01.T01:CDS 55.0%
TTCTCTCTGGTGCAGTGGAG+AGG + Chr4:81623942-81623961 MsG0480023162.01.T01:CDS 55.0%
ACTGGGACCGTGCGGTCTCA+AGG + Chr4:81623837-81623856 MsG0480023162.01.T01:CDS 65.0%
!! TGGGGAGCACTGGGACCGTG+CGG + Chr4:81623829-81623848 MsG0480023162.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 81623819 81624055 81623819 ID=MsG0480023162.01;Name=MsG0480023162.01
Chr4 mRNA 81623819 81624055 81623819 ID=MsG0480023162.01.T01;Parent=MsG0480023162.01;Name=MsG0480023162.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|78
Chr4 exon 81623819 81624055 81623819 ID=MsG0480023162.01.T01:exon:699;Parent=MsG0480023162.01.T01
Chr4 CDS 81623819 81624055 81623819 ID=MsG0480023162.01.T01:cds;Parent=MsG0480023162.01.T01
Gene Sequence

>MsG0480023162.01.T01

TTGGTATTAGTGGGGAGCACTGGGACCGTGCGGTCTCAAGGTGATTCTGCTTATGGAGCCAATGTTGAAGTGAGGCTTAGAGAGGCAGATTTTCCAATTGGGCAGGATCAGTCTTCATTGAGTTTCTCTCTGGTGCAGTGGAGAGGTGATTTAGCCGTGGGAGCCAATTTCCAGTCACAGATTTCTCTTGGCGAAGCTATAAGATGGCGGTTCGTGCGGATTGAACAACAAGCTTAG

Protein sequence

>MsG0480023162.01.T01

LVLVGSTGTVRSQGDSAYGANVEVRLREADFPIGQDQSSLSFSLVQWRGDLAVGANFQSQISLGEAIRWRFVRIEQQA*