AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045387.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045387.01.T01 MTR_8g070510 93.467 199 6 1 1 199 1 192 1.07e-133 374
MsG0880045387.01.T01 MTR_8g070510 93.333 195 6 1 5 199 1 188 2.56e-130 365
MsG0880045387.01.T01 MTR_7g451050 34.973 183 110 3 18 200 18 191 5.68e-34 120
MsG0880045387.01.T01 MTR_7g451050 34.973 183 110 3 18 200 36 209 7.01e-34 120
MsG0880045387.01.T01 MTR_7g451050 35.165 182 109 3 18 199 18 190 8.92e-34 120
MsG0880045387.01.T01 MTR_7g451050 35.165 182 109 3 18 199 36 208 1.35e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045387.01.T01 AT4G26470 69.000 200 53 2 1 200 1 191 3.50e-97 281
MsG0880045387.01.T01 AT4G26470 68.844 199 53 2 1 199 1 190 1.01e-95 278
MsG0880045387.01.T01 AT4G26470 68.844 199 53 2 1 199 64 253 1.41e-94 278
MsG0880045387.01.T01 AT3G24110 39.378 193 107 4 9 199 9 193 7.45e-38 131
MsG0880045387.01.T01 AT3G24110 39.378 193 107 4 9 199 12 196 7.63e-38 131

Find 40 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATGTGGCCTCCAGATT+TGG 0.325162 8:-58649148 None:intergenic
AACCAAGCTTGAGGCTAAAA+TGG 0.325884 8:+58647495 MsG0880045387.01.T01:CDS
ACATCTGGGGAACGTTCTTC+TGG 0.334302 8:+58649259 MsG0880045387.01.T01:CDS
ACGAATTGGCATGCCAAATC+TGG 0.337140 8:+58649135 MsG0880045387.01.T01:CDS
GGTAGGTTTCTACTTCAAAT+TGG 0.337511 8:+58647640 MsG0880045387.01.T01:CDS
TATAGTGTTGAATGATTTCA+TGG 0.344014 8:-58647554 None:intergenic
AGAATAGCCATGAAAAGATT+TGG 0.372821 8:+58649283 MsG0880045387.01.T01:CDS
GCCGCAGATGAAGATTCTAA+TGG 0.378648 8:+58648294 MsG0880045387.01.T01:intron
GCTATAAATGAAACTACATC+TGG 0.389448 8:+58649244 MsG0880045387.01.T01:CDS
CCGCAGATGAAGATTCTAAT+GGG 0.395441 8:+58648295 MsG0880045387.01.T01:intron
AGGGCCATCTTTGAGCAGTT+TGG 0.430873 8:+58647619 MsG0880045387.01.T01:CDS
CTTGTCTAGAAATCACGAAT+TGG 0.446229 8:+58649121 MsG0880045387.01.T01:intron
ATGTAGTTTCATTTATAGCC+TGG 0.447264 8:-58649240 None:intergenic
GAAGAAGTGTTTCAGTAAGC+TGG 0.478339 8:+58648338 MsG0880045387.01.T01:CDS
ATGGGAGGTGCAATGGGAAA+GGG 0.479453 8:+58647442 MsG0880045387.01.T01:CDS
GATACTGCATGAAGTGCTGC+AGG 0.484247 8:-58648486 None:intergenic
GATGGGAGGTGCAATGGGAA+AGG 0.497322 8:+58647441 None:intergenic
GGAGGCCACATTTGAGACAT+TGG 0.509823 8:+58649156 MsG0880045387.01.T01:CDS
TTGGCGATGGGAGGTGCAAT+GGG 0.518653 8:+58647436 None:intergenic
ATTGGCGATGGGAGGTGCAA+TGG 0.524730 8:+58647435 None:intergenic
CCATCTTTGAGCAGTTTGGT+AGG 0.526543 8:+58647623 MsG0880045387.01.T01:CDS
ATGAGAGCTTCAGAAAATGC+AGG 0.530176 8:+58647599 MsG0880045387.01.T01:CDS
CCTACCAAACTGCTCAAAGA+TGG 0.539084 8:-58647623 None:intergenic
CTATAAATGAAACTACATCT+GGG 0.544055 8:+58649245 MsG0880045387.01.T01:CDS
CTGCAGGGTCATCTTTGAGA+AGG 0.604557 8:-58648470 None:intergenic
CAAGCTTGAGGCTAAAATGG+TGG 0.616956 8:+58647498 MsG0880045387.01.T01:CDS
GTTGATTGATTGGCGATGGG+AGG 0.623063 8:+58647427 None:intergenic
TGGTGGAAGCAATGCAACGG+AGG 0.623952 8:+58647515 MsG0880045387.01.T01:CDS
ATCAACCAATGTCTCAAATG+TGG 0.635646 8:-58649161 None:intergenic
ATATGTGTGGAATTTAAACA+AGG 0.640636 8:-58649318 None:intergenic
TGAGAGCTTCAGAAAATGCA+GGG 0.640702 8:+58647600 MsG0880045387.01.T01:CDS
GGTGGAAGCAATGCAACGGA+GGG 0.644209 8:+58647516 MsG0880045387.01.T01:CDS
AATTGGCATGCCAAATCTGG+AGG 0.644569 8:+58649138 MsG0880045387.01.T01:CDS
CGCAGATGAAGATTCTAATG+GGG 0.650490 8:+58648296 MsG0880045387.01.T01:intron
TATAAATGAAACTACATCTG+GGG 0.652827 8:+58649246 MsG0880045387.01.T01:CDS
GGTAGCAGAAACCAAGCTTG+AGG 0.658820 8:+58647486 MsG0880045387.01.T01:CDS
AAATGGTGGAAGCAATGCAA+CGG 0.672556 8:+58647512 MsG0880045387.01.T01:CDS
ATGCAACGGAGGGAATGTCA+AGG 0.680566 8:+58647526 MsG0880045387.01.T01:CDS
TATGTGTGGAATTTAAACAA+GGG 0.682963 8:-58649317 None:intergenic
ATACTGCATGAAGTGCTGCA+GGG 0.692431 8:-58648485 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAAAATTTCAAAATTAT+AGG - Chr8:58648964-58648983 None:intergenic 10.0%
!! ACAATCACATAAAAAAAAAA+TGG - Chr8:58648134-58648153 None:intergenic 15.0%
!! CTGATATAAATATAGTTTAT+TGG + Chr8:58647695-58647714 MsG0880045387.01.T01:intron 15.0%
!! GAAAATTACATATTTAATTC+AGG + Chr8:58648844-58648863 MsG0880045387.01.T01:intron 15.0%
!! GTATATATGTTTATTGTAAT+TGG + Chr8:58648246-58648265 MsG0880045387.01.T01:intron 15.0%
!!! GAATATTATTTGAGTTTAAT+GGG + Chr8:58648609-58648628 MsG0880045387.01.T01:intron 15.0%
!!! TAACTGCATTTTTAAATTAA+TGG - Chr8:58648704-58648723 None:intergenic 15.0%
!!! TAAGCTATTTTTTTAACAAA+AGG + Chr8:58647853-58647872 MsG0880045387.01.T01:intron 15.0%
!!! TTTTCTAAATAAAATGCAAA+AGG - Chr8:58648829-58648848 None:intergenic 15.0%
!! AAAAATGCAGTTATAAAAGT+GGG + Chr8:58648711-58648730 MsG0880045387.01.T01:intron 20.0%
!! CAAAAATAGTATGGATTTAA+AGG - Chr8:58648805-58648824 None:intergenic 20.0%
!! TAAAAATGCAGTTATAAAAG+TGG + Chr8:58648710-58648729 MsG0880045387.01.T01:intron 20.0%
!! TAAACATATATACGACAATT+TGG - Chr8:58648239-58648258 None:intergenic 20.0%
!! TATAAATTATCAACGAAAAC+AGG - Chr8:58649064-58649083 None:intergenic 20.0%
!!! ATGTGATTTTAATGATGATT+TGG + Chr8:58648404-58648423 MsG0880045387.01.T01:CDS 20.0%
!!! GGAATATTATTTGAGTTTAA+TGG + Chr8:58648608-58648627 MsG0880045387.01.T01:intron 20.0%
!!! TGTATTTTTAGACAAGAATA+AGG + Chr8:58649189-58649208 MsG0880045387.01.T01:CDS 20.0%
!!! TGTGATTTTAATGATGATTT+GGG + Chr8:58648405-58648424 MsG0880045387.01.T01:CDS 20.0%
! AATGGTATGACATGAAAAAA+TGG - Chr8:58648686-58648705 None:intergenic 25.0%
! ATATAAGCATTTACATCACA+AGG - Chr8:58647832-58647851 None:intergenic 25.0%
! ATTTGAAAGGATAGAGAAAA+TGG - Chr8:58649089-58649108 None:intergenic 25.0%
! CTATAAATGAAACTACATCT+GGG + Chr8:58649245-58649264 MsG0880045387.01.T01:CDS 25.0%
! GTTGATGTTTGAATTATTTC+GGG + Chr8:58648559-58648578 MsG0880045387.01.T01:intron 25.0%
! TATAAATGAAACTACATCTG+GGG + Chr8:58649246-58649265 MsG0880045387.01.T01:CDS 25.0%
! TATTTAATTCAGGAGATGTT+TGG + Chr8:58648854-58648873 MsG0880045387.01.T01:intron 25.0%
! TTTATTTCCATAAACTCTCT+AGG - Chr8:58647931-58647950 None:intergenic 25.0%
!! ATACATTTAACTTCAGAGAA+TGG + Chr8:58648587-58648606 MsG0880045387.01.T01:intron 25.0%
!! GGTTGATGTTTGAATTATTT+CGG + Chr8:58648558-58648577 MsG0880045387.01.T01:intron 25.0%
!! GTTAAGAAGTTTTTCTGTTA+TGG + Chr8:58648903-58648922 MsG0880045387.01.T01:intron 25.0%
!! TATAGTGTTGAATGATTTCA+TGG - Chr8:58647557-58647576 None:intergenic 25.0%
!! TTAAGTTGTTTACTCAAACA+TGG - Chr8:58647783-58647802 None:intergenic 25.0%
!! TTTTAGACAAGAATAAGGAT+GGG + Chr8:58649194-58649213 MsG0880045387.01.T01:CDS 25.0%
!! TTTTTAGACAAGAATAAGGA+TGG + Chr8:58649193-58649212 MsG0880045387.01.T01:CDS 25.0%
!!! CATCAATTTTTGGGAATTTT+AGG - Chr8:58647582-58647601 None:intergenic 25.0%
!!! TGCTTTTGAAAACAACTTAT+AGG - Chr8:58648511-58648530 None:intergenic 25.0%
AGAATAGCCATGAAAAGATT+TGG + Chr8:58649283-58649302 MsG0880045387.01.T01:CDS 30.0%
ATAATTCAAACATCAACCAG+AGG - Chr8:58648556-58648575 None:intergenic 30.0%
ATGTAGTTTCATTTATAGCC+TGG - Chr8:58649243-58649262 None:intergenic 30.0%
CAATTATCAAGCTCATTCAA+AGG + Chr8:58648159-58648178 MsG0880045387.01.T01:intron 30.0%
GCAAAAGGACAAAAATAGTA+TGG - Chr8:58648814-58648833 None:intergenic 30.0%
GCTATAAATGAAACTACATC+TGG + Chr8:58649244-58649263 MsG0880045387.01.T01:CDS 30.0%
GTATGTCAGCAAAAATGAAA+TGG + Chr8:58649216-58649235 MsG0880045387.01.T01:CDS 30.0%
GTCATAGAGAACAATTTGAA+AGG - Chr8:58649102-58649121 None:intergenic 30.0%
TGAATTTATACACTAGGCTA+GGG + Chr8:58647720-58647739 MsG0880045387.01.T01:intron 30.0%
TTATAAAAGTGGGATGATGT+GGG + Chr8:58648721-58648740 MsG0880045387.01.T01:intron 30.0%
! ATAAACTGAAAACCAGCTTT+TGG + Chr8:58647946-58647965 MsG0880045387.01.T01:intron 30.0%
! CTAATTTACACCGACATTTT+AGG - Chr8:58648635-58648654 None:intergenic 30.0%
! TGAAGCTCTCATCAATTTTT+GGG - Chr8:58647591-58647610 None:intergenic 30.0%
! TGAATCACCCAAAAAGTTTT+GGG + Chr8:58647465-58647484 MsG0880045387.01.T01:CDS 30.0%
AAAAAATGGAGGACTCTCAT+TGG - Chr8:58648672-58648691 None:intergenic 35.0%
AAGCTATCCTAGAGAGTTTA+TGG + Chr8:58647921-58647940 MsG0880045387.01.T01:intron 35.0%
ACTGACATAAGCACTTGTTT+GGG - Chr8:58648013-58648032 None:intergenic 35.0%
ATCAACCAATGTCTCAAATG+TGG - Chr8:58649164-58649183 None:intergenic 35.0%
CATTAACCTATGTTTGTCAC+CGG + Chr8:58648202-58648221 MsG0880045387.01.T01:intron 35.0%
CTAGCCTAGTGTATAAATTC+AGG - Chr8:58647721-58647740 None:intergenic 35.0%
CTGAATTTATACACTAGGCT+AGG + Chr8:58647719-58647738 MsG0880045387.01.T01:intron 35.0%
CTTGTCTAGAAATCACGAAT+TGG + Chr8:58649121-58649140 MsG0880045387.01.T01:intron 35.0%
GGTAGGTTTCTACTTCAAAT+TGG + Chr8:58647640-58647659 MsG0880045387.01.T01:CDS 35.0%
GGTATGACATGAAAAAATGG+AGG - Chr8:58648683-58648702 None:intergenic 35.0%
GTTATAAAAGTGGGATGATG+TGG + Chr8:58648720-58648739 MsG0880045387.01.T01:intron 35.0%
TACTGACATAAGCACTTGTT+TGG - Chr8:58648014-58648033 None:intergenic 35.0%
TTGGCCTGAATTTATACACT+AGG + Chr8:58647714-58647733 MsG0880045387.01.T01:intron 35.0%
! ACACTGACCAAATCTTTTCA+TGG - Chr8:58649293-58649312 None:intergenic 35.0%
! ACATTGATTGAGAGAGCTTA+TGG - Chr8:58647990-58648009 None:intergenic 35.0%
! CTGAAGCTCTCATCAATTTT+TGG - Chr8:58647592-58647611 None:intergenic 35.0%
! GCTGGAAATTTCTTTTTCTG+AGG + Chr8:58648356-58648375 MsG0880045387.01.T01:CDS 35.0%
! GTGAATCACCCAAAAAGTTT+TGG + Chr8:58647464-58647483 MsG0880045387.01.T01:CDS 35.0%
! GTTTAATGGGCCTAAAATGT+CGG + Chr8:58648622-58648641 MsG0880045387.01.T01:intron 35.0%
! TCTGCTACCCAAAACTTTTT+GGG - Chr8:58647475-58647494 None:intergenic 35.0%
! TTCTGCTACCCAAAACTTTT+TGG - Chr8:58647476-58647495 None:intergenic 35.0%
AACCAAGCTTGAGGCTAAAA+TGG + Chr8:58647495-58647514 MsG0880045387.01.T01:CDS 40.0%
AGCAATGCAATGTAGTAGGA+AGG + Chr8:58649015-58649034 MsG0880045387.01.T01:intron 40.0%
ATGAGAGCTTCAGAAAATGC+AGG + Chr8:58647599-58647618 MsG0880045387.01.T01:CDS 40.0%
CAGCAAAAATGAAATGGTCC+AGG + Chr8:58649222-58649241 MsG0880045387.01.T01:CDS 40.0%
CCCATTAGAATCTTCATCTG+CGG - Chr8:58648298-58648317 None:intergenic 40.0%
CCGCAGATGAAGATTCTAAT+GGG + Chr8:58648295-58648314 MsG0880045387.01.T01:intron 40.0%
CGCAGATGAAGATTCTAATG+GGG + Chr8:58648296-58648315 MsG0880045387.01.T01:intron 40.0%
CTGAAGCAATGCAATGTAGT+AGG + Chr8:58649011-58649030 MsG0880045387.01.T01:intron 40.0%
GCCAAAGTAAAAGCAAGAAG+TGG + Chr8:58647747-58647766 MsG0880045387.01.T01:intron 40.0%
TCATTCAAAGGCTTCAACAG+TGG + Chr8:58648171-58648190 MsG0880045387.01.T01:intron 40.0%
TGAGAGCTTCAGAAAATGCA+GGG + Chr8:58647600-58647619 MsG0880045387.01.T01:CDS 40.0%
! AAATGGTGGAAGCAATGCAA+CGG + Chr8:58647512-58647531 MsG0880045387.01.T01:CDS 40.0%
!! GAAGAAGTGTTTCAGTAAGC+TGG + Chr8:58648338-58648357 MsG0880045387.01.T01:CDS 40.0%
!! GCCACTTCTTGCTTTTACTT+TGG - Chr8:58647751-58647770 None:intergenic 40.0%
AATTGGCATGCCAAATCTGG+AGG + Chr8:58649138-58649157 MsG0880045387.01.T01:CDS 45.0%
ACGAATTGGCATGCCAAATC+TGG + Chr8:58649135-58649154 MsG0880045387.01.T01:CDS 45.0%
CAAGCTTGAGGCTAAAATGG+TGG + Chr8:58647498-58647517 MsG0880045387.01.T01:CDS 45.0%
CATGAAACACTGCGGCTATT+CGG + Chr8:58648071-58648090 MsG0880045387.01.T01:intron 45.0%
CCATCTTTGAGCAGTTTGGT+AGG + Chr8:58647623-58647642 MsG0880045387.01.T01:CDS 45.0%
CCTACCAAACTGCTCAAAGA+TGG - Chr8:58647626-58647645 None:intergenic 45.0%
CTTACGACATGTCCAAAAGC+TGG - Chr8:58647961-58647980 None:intergenic 45.0%
GCCGCAGATGAAGATTCTAA+TGG + Chr8:58648294-58648313 MsG0880045387.01.T01:intron 45.0%
GCCTAGATCATGAAACACTG+CGG + Chr8:58648063-58648082 MsG0880045387.01.T01:intron 45.0%
GGGATGATGTGGGAAAATAG+TGG + Chr8:58648731-58648750 MsG0880045387.01.T01:intron 45.0%
TCAAATGTGGCCTCCAGATT+TGG - Chr8:58649151-58649170 None:intergenic 45.0%
!! ATACTGCATGAAGTGCTGCA+GGG - Chr8:58648488-58648507 None:intergenic 45.0%
!! CACCATTTTAGCCTCAAGCT+TGG - Chr8:58647500-58647519 None:intergenic 45.0%
ACATCTGGGGAACGTTCTTC+TGG + Chr8:58649259-58649278 MsG0880045387.01.T01:CDS 50.0%
AGGGCCATCTTTGAGCAGTT+TGG + Chr8:58647619-58647638 MsG0880045387.01.T01:CDS 50.0%
ATGCAACGGAGGGAATGTCA+AGG + Chr8:58647526-58647545 MsG0880045387.01.T01:CDS 50.0%
CAATTTGGCCATGCATGTGC+CGG - Chr8:58648224-58648243 None:intergenic 50.0%
GCCGCAGTGTTTCATGATCT+AGG - Chr8:58648067-58648086 None:intergenic 50.0%
GGTAGCAGAAACCAAGCTTG+AGG + Chr8:58647486-58647505 MsG0880045387.01.T01:CDS 50.0%
GTCTTGTGCATTCTGACCTC+TGG + Chr8:58648537-58648556 MsG0880045387.01.T01:intron 50.0%
! ATGGGAGGTGCAATGGGAAA+GGG + Chr8:58647442-58647461 MsG0880045387.01.T01:CDS 50.0%
! CATGTGCCGGTGACAAACAT+AGG - Chr8:58648211-58648230 None:intergenic 50.0%
! CTGCAGGGTCATCTTTGAGA+AGG - Chr8:58648473-58648492 None:intergenic 50.0%
! GGAGGCCACATTTGAGACAT+TGG + Chr8:58649156-58649175 MsG0880045387.01.T01:CDS 50.0%
!! GATACTGCATGAAGTGCTGC+AGG - Chr8:58648489-58648508 None:intergenic 50.0%
GGTGGAAGCAATGCAACGGA+GGG + Chr8:58647516-58647535 MsG0880045387.01.T01:CDS 55.0%
GTTTGTCACCGGCACATGCA+TGG + Chr8:58648213-58648232 MsG0880045387.01.T01:intron 55.0%
TGGTGGAAGCAATGCAACGG+AGG + Chr8:58647515-58647534 MsG0880045387.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 58647442 58649327 58647442 ID=MsG0880045387.01;Name=MsG0880045387.01
Chr8 mRNA 58647442 58649327 58647442 ID=MsG0880045387.01.T01;Parent=MsG0880045387.01;Name=MsG0880045387.01.T01;_AED=0.43;_eAED=0.47;_QI=0|0.5|0.33|1|1|1|3|0|206
Chr8 exon 58647442 58647661 58647442 ID=MsG0880045387.01.T01:exon:12037;Parent=MsG0880045387.01.T01
Chr8 exon 58648301 58648503 58648301 ID=MsG0880045387.01.T01:exon:12038;Parent=MsG0880045387.01.T01
Chr8 exon 58649130 58649327 58649130 ID=MsG0880045387.01.T01:exon:12039;Parent=MsG0880045387.01.T01
Chr8 CDS 58647442 58647661 58647442 ID=MsG0880045387.01.T01:cds;Parent=MsG0880045387.01.T01
Chr8 CDS 58648301 58648503 58648301 ID=MsG0880045387.01.T01:cds;Parent=MsG0880045387.01.T01
Chr8 CDS 58649130 58649327 58649130 ID=MsG0880045387.01.T01:cds;Parent=MsG0880045387.01.T01
Gene Sequence

>MsG0880045387.01.T01

ATGGGAGGTGCAATGGGAAAGGGTGAATCACCCAAAAAGTTTTGGGTAGCAGAAACCAAGCTTGAGGCTAAAATGGTGGAAGCAATGCAACGGAGGGAATGTCAAGGATGTTCCATGAAATCATTCAACACTATAATCCTAAAATTCCCAAAAATTGATGAGAGCTTCAGAAAATGCAGGGCCATCTTTGAGCAGTTTGGTAGGTTTCTACTTCAAATTGATGAAGATTCTAATGGGGCAATAGATCATGAAGAGTTGAAGAAGTGTTTCAGTAAGCTGGAAATTTCTTTTTCTGAGGAAGAGATCAATGATCTTTTTGAAGCATGTGATTTTAATGATGATTTGGGAATGAAGTTCAATGAGTTCATTGTACTTCTTTGTCTTGTCTACCTTCTCAAAGATGACCCTGCAGCACTTCATGCAAAATCACGAATTGGCATGCCAAATCTGGAGGCCACATTTGAGACATTGGTTGATACATTTGTATTTTTAGACAAGAATAAGGATGGGTATGTCAGCAAAAATGAAATGGTCCAGGCTATAAATGAAACTACATCTGGGGAACGTTCTTCTGGAAGAATAGCCATGAAAAGATTTGGTCAGTGTCTCTCCCTTGTTTAA

Protein sequence

>MsG0880045387.01.T01

MGGAMGKGESPKKFWVAETKLEAKMVEAMQRRECQGCSMKSFNTIILKFPKIDESFRKCRAIFEQFGRFLLQIDEDSNGAIDHEELKKCFSKLEISFSEEEINDLFEACDFNDDLGMKFNEFIVLLCLVYLLKDDPAALHAKSRIGMPNLEATFETLVDTFVFLDKNKDGYVSKNEMVQAINETTSGERSSGRIAMKRFGQCLSLV*