Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029023.01.T02 | XP_003616435.1 | 97.59 | 83 | 2 | 0 | 1 | 83 | 7 | 89 | 4.28E-53 | 170 |
| MsG0580029023.01.T01 | XP_003616435.1 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 7.08E-56 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029023.01.T01 | Q8S8S3 | 62.667 | 75 | 25 | 2 | 18 | 89 | 13 | 87 | 1.02E-24 | 91.3 |
| MsG0580029023.01.T02 | Q8S8S3 | 62.667 | 75 | 25 | 2 | 12 | 83 | 13 | 87 | 1.36E-24 | 90.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029023.01.T01 | G7KGR6 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 3.38e-56 | 177 |
| MsG0580029023.01.T02 | G7KGR6 | 97.590 | 83 | 2 | 0 | 1 | 83 | 7 | 89 | 2.04e-53 | 170 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048186.01 | MsG0580029023.01 | 0.821314 | 4.313407e-53 | 3.497057e-50 |
| MsG0180001925.01 | MsG0580029023.01 | -0.803103 | 4.089355e-49 | 2.037294e-46 |
| MsG0180003898.01 | MsG0580029023.01 | 0.836045 | 1.177659e-56 | 1.466686e-53 |
| MsG0180004559.01 | MsG0580029023.01 | 0.806896 | 6.582288e-50 | 3.614947e-47 |
| MsG0180004761.01 | MsG0580029023.01 | -0.813581 | 2.381268e-51 | 1.561106e-48 |
| MsG0180005514.01 | MsG0580029023.01 | 0.835914 | 1.271351e-56 | 1.577025e-53 |
| MsG0280006420.01 | MsG0580029023.01 | 0.821930 | 3.107419e-53 | 2.563444e-50 |
| MsG0380012694.01 | MsG0580029023.01 | 0.829584 | 4.742725e-55 | 4.874827e-52 |
| MsG0380014754.01 | MsG0580029023.01 | 0.836582 | 8.600666e-57 | 1.088474e-53 |
| MsG0380014881.01 | MsG0580029023.01 | 0.829085 | 6.271658e-55 | 6.352264e-52 |
| MsG0480020263.01 | MsG0580029023.01 | 0.822841 | 1.908672e-53 | 1.616026e-50 |
| MsG0480021476.01 | MsG0580029023.01 | -0.825390 | 4.811846e-54 | 4.379801e-51 |
| MsG0480021912.01 | MsG0580029023.01 | -0.805351 | 1.392055e-49 | 7.348206e-47 |
| MsG0480022167.01 | MsG0580029023.01 | 0.817079 | 3.972467e-52 | 2.864581e-49 |
| MsG0480023324.01 | MsG0580029023.01 | 0.811014 | 8.652872e-51 | 5.297142e-48 |
| MsG0580024478.01 | MsG0580029023.01 | 0.861075 | 1.323375e-63 | 3.697828e-60 |
| MsG0580025575.01 | MsG0580029023.01 | 0.811664 | 6.252047e-51 | 3.892924e-48 |
| MsG0380016008.01 | MsG0580029023.01 | 0.802661 | 5.046857e-49 | 2.486206e-46 |
| MsG0380017231.01 | MsG0580029023.01 | 0.864405 | 1.245077e-64 | 3.904864e-61 |
| MsG0380017804.01 | MsG0580029023.01 | -0.865194 | 7.045702e-65 | 2.272662e-61 |
| MsG0380017935.01 | MsG0580029023.01 | -0.812852 | 3.441008e-51 | 2.211728e-48 |
| MsG0580027626.01 | MsG0580029023.01 | 0.801144 | 1.034358e-48 | 4.903338e-46 |
| MsG0580028330.01 | MsG0580029023.01 | 0.807437 | 5.058222e-50 | 2.817211e-47 |
| MsG0580028478.01 | MsG0580029023.01 | 0.816011 | 6.888410e-52 | 4.824505e-49 |
| MsG0580029023.01 | MsG0580029623.01 | 0.842367 | 2.700598e-58 | 4.085861e-55 |
| MsG0580029023.01 | MsG0580029792.01 | -0.816203 | 6.242130e-52 | 4.394870e-49 |
| MsG0580029023.01 | MsG0680031178.01 | 0.818191 | 2.229317e-52 | 1.657394e-49 |
| MsG0580029023.01 | MsG0680033623.01 | 0.814043 | 1.883345e-51 | 1.250255e-48 |
| MsG0580029023.01 | MsG0680034137.01 | 0.804946 | 1.692497e-49 | 8.842225e-47 |
| MsG0580029023.01 | MsG0780039462.01 | -0.803240 | 3.830316e-49 | 1.915054e-46 |
| MsG0580029023.01 | MsG0780040266.01 | 0.843911 | 1.047326e-58 | 1.663283e-55 |
| MsG0580029023.01 | MsG0780041531.01 | -0.815674 | 8.189146e-52 | 5.682887e-49 |
| MsG0580029023.01 | MsG0780041637.01 | -0.805835 | 1.101848e-49 | 5.889277e-47 |
| MsG0580029023.01 | MsG0880041883.01 | 0.830099 | 3.553925e-55 | 3.707637e-52 |
| MsG0580029023.01 | MsG0880043261.01 | 0.839727 | 1.332961e-57 | 1.857066e-54 |
| MsG0580029023.01 | MsG0880043983.01 | 0.800101 | 1.687396e-48 | 7.790075e-46 |
| MsG0580029023.01 | MsG0880045808.01 | 0.818805 | 1.618070e-52 | 1.223466e-49 |
| MsG0580029023.01 | MsG0880046167.01 | -0.800171 | 1.633369e-48 | 7.553861e-46 |
| MsG0280008820.01 | MsG0580029023.01 | -0.809131 | 2.201527e-50 | 1.282083e-47 |
| MsG0280010296.01 | MsG0580029023.01 | 0.802056 | 6.723931e-49 | 3.261831e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029023.01.T02 | MTR_5g080260 | 97.590 | 83 | 2 | 0 | 1 | 83 | 7 | 89 | 5.18e-57 | 170 |
| MsG0580029023.01.T02 | MTR_3g067980 | 67.949 | 78 | 24 | 1 | 7 | 83 | 16 | 93 | 2.25e-31 | 105 |
| MsG0580029023.01.T02 | MTR_4g064520 | 36.275 | 102 | 32 | 2 | 14 | 82 | 1 | 102 | 1.88e-14 | 63.2 |
| MsG0580029023.01.T02 | MTR_2g097570 | 61.111 | 36 | 14 | 0 | 48 | 83 | 51 | 86 | 1.21e-12 | 58.2 |
| MsG0580029023.01.T02 | MTR_7g101850 | 41.538 | 65 | 29 | 2 | 22 | 82 | 4 | 63 | 2.99e-11 | 53.9 |
| MsG0580029023.01.T02 | MTR_8g023610 | 57.895 | 38 | 16 | 0 | 40 | 77 | 15 | 52 | 4.03e-11 | 53.5 |
| MsG0580029023.01.T01 | MTR_5g080260 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 8.57e-60 | 177 |
| MsG0580029023.01.T01 | MTR_3g067980 | 70.667 | 75 | 21 | 1 | 16 | 89 | 19 | 93 | 1.84e-31 | 106 |
| MsG0580029023.01.T01 | MTR_4g064520 | 36.275 | 102 | 32 | 2 | 20 | 88 | 1 | 102 | 2.07e-14 | 63.2 |
| MsG0580029023.01.T01 | MTR_2g097570 | 61.111 | 36 | 14 | 0 | 54 | 89 | 51 | 86 | 1.21e-12 | 58.2 |
| MsG0580029023.01.T01 | MTR_7g101850 | 41.538 | 65 | 29 | 2 | 28 | 88 | 4 | 63 | 3.00e-11 | 53.9 |
| MsG0580029023.01.T01 | MTR_8g023610 | 57.895 | 38 | 16 | 0 | 46 | 83 | 15 | 52 | 4.30e-11 | 53.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029023.01.T02 | AT2G39705 | 62.667 | 75 | 25 | 2 | 12 | 83 | 13 | 87 | 1.39e-25 | 90.9 |
| MsG0580029023.01.T02 | AT3G55515 | 52.239 | 67 | 31 | 1 | 17 | 83 | 9 | 74 | 1.24e-18 | 73.2 |
| MsG0580029023.01.T02 | AT2G36985 | 61.290 | 31 | 12 | 0 | 49 | 79 | 15 | 45 | 1.83e-11 | 54.3 |
| MsG0580029023.01.T02 | AT3G14362 | 65.714 | 35 | 12 | 0 | 48 | 82 | 13 | 47 | 3.64e-11 | 53.1 |
| MsG0580029023.01.T02 | AT4G13395 | 47.059 | 51 | 24 | 1 | 36 | 83 | 4 | 54 | 7.42e-11 | 52.8 |
| MsG0580029023.01.T02 | AT1G53708 | 68.571 | 35 | 11 | 0 | 48 | 82 | 13 | 47 | 9.94e-11 | 53.5 |
| MsG0580029023.01.T01 | AT2G39705 | 62.667 | 75 | 25 | 2 | 18 | 89 | 13 | 87 | 1.04e-25 | 91.3 |
| MsG0580029023.01.T01 | AT3G55515 | 52.239 | 67 | 31 | 1 | 23 | 89 | 9 | 74 | 1.11e-18 | 73.6 |
| MsG0580029023.01.T01 | AT2G36985 | 61.290 | 31 | 12 | 0 | 55 | 85 | 15 | 45 | 1.74e-11 | 54.3 |
| MsG0580029023.01.T01 | AT3G14362 | 65.714 | 35 | 12 | 0 | 54 | 88 | 13 | 47 | 4.02e-11 | 53.5 |
| MsG0580029023.01.T01 | AT4G13395 | 47.059 | 51 | 24 | 1 | 42 | 89 | 4 | 54 | 8.18e-11 | 52.8 |
Find 11 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGGCTTCTTCTTCTTATA+TGG | 0.110788 | 5:+90532785 | None:intergenic |
| TAGAGAGGAGGTTGAGGTTG+AGG | 0.358701 | 5:-90532819 | None:intergenic |
| AGAGAGGAGGTTGAGGTTGA+GGG | 0.382443 | 5:-90532818 | None:intergenic |
| GTGTTACAATGCTCATTTGC+TGG | 0.423945 | 5:+90533012 | MsG0580029023.01.T01:CDS |
| TGGAAGCTATCAAAGAAAGA+AGG | 0.500507 | 5:+90532855 | MsG0580029023.01.T01:CDS |
| TCAAAGTAGAGAGGAGGTTG+AGG | 0.504111 | 5:-90532825 | None:intergenic |
| GAGAGGAGGTTGAGGTTGAG+GGG | 0.511193 | 5:-90532817 | None:intergenic |
| CATATAAGAAGAAGAAGCCA+TGG | 0.564875 | 5:-90532784 | None:intergenic |
| GGAAGCTATCAAAGAAAGAA+GGG | 0.569563 | 5:+90532856 | MsG0580029023.01.T01:CDS |
| CACGTTTCTACATCATGAGG+CGG | 0.662634 | 5:+90532988 | MsG0580029023.01.T01:CDS |
| GAGCACGTTTCTACATCATG+AGG | 0.665259 | 5:+90532985 | MsG0580029023.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCTCTACTTTGATGAAAAA+TGG | + | Chr5:90532835-90532854 | MsG0580029023.01.T01:CDS | 30.0% | |
| ! | CATTTTTCATCAAAGTAGAG+AGG | - | Chr5:90532837-90532856 | None:intergenic | 30.0% |
| GGAAGCTATCAAAGAAAGAA+GGG | + | Chr5:90532856-90532875 | MsG0580029023.01.T01:CDS | 35.0% | |
| TGGAAGCTATCAAAGAAAGA+AGG | + | Chr5:90532855-90532874 | MsG0580029023.01.T01:CDS | 35.0% | |
| ! | TTTTCATCAAAGTAGAGAGG+AGG | - | Chr5:90532834-90532853 | None:intergenic | 35.0% |
| GTGTTACAATGCTCATTTGC+TGG | + | Chr5:90533012-90533031 | MsG0580029023.01.T01:CDS | 40.0% | |
| CACGTTTCTACATCATGAGG+CGG | + | Chr5:90532988-90533007 | MsG0580029023.01.T01:CDS | 45.0% | |
| GAGCACGTTTCTACATCATG+AGG | + | Chr5:90532985-90533004 | MsG0580029023.01.T01:CDS | 45.0% | |
| TCAAAGTAGAGAGGAGGTTG+AGG | - | Chr5:90532828-90532847 | None:intergenic | 45.0% | |
| AGAGAGGAGGTTGAGGTTGA+GGG | - | Chr5:90532821-90532840 | None:intergenic | 50.0% | |
| TAGAGAGGAGGTTGAGGTTG+AGG | - | Chr5:90532822-90532841 | None:intergenic | 50.0% | |
| GAGAGGAGGTTGAGGTTGAG+GGG | - | Chr5:90532820-90532839 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 90532786 | 90533055 | 90532786 | ID=MsG0580029023.01;Name=MsG0580029023.01 |
| Chr5 | mRNA | 90532786 | 90533055 | 90532786 | ID=MsG0580029023.01.T01;Parent=MsG0580029023.01;Name=MsG0580029023.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|89 |
| Chr5 | exon | 90532786 | 90533055 | 90532786 | ID=MsG0580029023.01.T01:exon:17665;Parent=MsG0580029023.01.T01 |
| Chr5 | CDS | 90532786 | 90533055 | 90532786 | ID=MsG0580029023.01.T01:cds;Parent=MsG0580029023.01.T01 |
| Chr5 | mRNA | 90532804 | 90533055 | 90532804 | ID=MsG0580029023.01.T02;Parent=MsG0580029023.01;Name=MsG0580029023.01.T02;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|0|1|0|83 |
| Chr5 | exon | 90532804 | 90533055 | 90532804 | ID=MsG0580029023.01.T02:exon:17666;Parent=MsG0580029023.01.T02 |
| Chr5 | CDS | 90532804 | 90533055 | 90532804 | ID=MsG0580029023.01.T02:cds;Parent=MsG0580029023.01.T02 |
| Chr5 | mRNA | 90532847 | 90533005 | 90532847 | ID=MsG0580029023.01.T03;Parent=MsG0580029023.01;Name=MsG0580029023.01.T03;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|0|1|0|52 |
| Chr5 | exon | 90532847 | 90533005 | 90532847 | ID=MsG0580029023.01.T03:exon:17667;Parent=MsG0580029023.01.T03 |
| Chr5 | CDS | 90532847 | 90533005 | 90532847 | ID=MsG0580029023.01.T03:cds;Parent=MsG0580029023.01.T03 |
| Chr5 | mRNA | 90532959 | 90533039 | 90532959 | ID=MsG0580029023.01.T04;Parent=MsG0580029023.01;Name=MsG0580029023.01.T04;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|26 |
| Chr5 | exon | 90532959 | 90533039 | 90532959 | ID=MsG0580029023.01.T04:exon:17668;Parent=MsG0580029023.01.T04 |
| Chr5 | CDS | 90532959 | 90533039 | 90532959 | ID=MsG0580029023.01.T04:cds;Parent=MsG0580029023.01.T04 |
| Gene Sequence |
| Protein sequence |
