Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029522.01.T01 | XP_003617086.1 | 97.297 | 74 | 2 | 0 | 20 | 93 | 421 | 494 | 2.85E-41 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029522.01.T01 | O24606 | 52 | 75 | 28 | 3 | 19 | 93 | 415 | 481 | 8.88E-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029522.01.T01 | G7K2Y0 | 97.297 | 74 | 2 | 0 | 20 | 93 | 421 | 494 | 1.36e-41 | 154 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048703.01 | MsG0580029522.01 | 0.821049 | 4.964328e-53 | 3.995675e-50 |
| MsG0180003767.01 | MsG0580029522.01 | 0.815519 | 8.870864e-52 | 6.129757e-49 |
| MsG0180003942.01 | MsG0580029522.01 | 0.815008 | 1.151675e-51 | 7.847768e-49 |
| MsG0180004954.01 | MsG0580029522.01 | 0.821301 | 4.343135e-53 | 3.519878e-50 |
| MsG0180005395.01 | MsG0580029522.01 | 0.833928 | 4.023034e-56 | 4.702120e-53 |
| MsG0180005918.01 | MsG0580029522.01 | 0.817777 | 2.765852e-52 | 2.033074e-49 |
| MsG0280011440.01 | MsG0580029522.01 | 0.853043 | 3.101681e-61 | 6.612979e-58 |
| MsG0480018644.01 | MsG0580029522.01 | -0.817128 | 3.872982e-52 | 2.796570e-49 |
| MsG0480018676.01 | MsG0580029522.01 | -0.815921 | 7.214232e-52 | 5.039916e-49 |
| MsG0480022275.01 | MsG0580029522.01 | 0.840347 | 9.182283e-58 | 1.304205e-54 |
| MsG0580025187.01 | MsG0580029522.01 | 0.809076 | 2.261955e-50 | 1.315307e-47 |
| MsG0580025369.01 | MsG0580029522.01 | 0.809767 | 1.608305e-50 | 9.526060e-48 |
| MsG0580025561.01 | MsG0580029522.01 | 0.800610 | 1.329238e-48 | 6.216353e-46 |
| MsG0380016188.01 | MsG0580029522.01 | 0.834234 | 3.372034e-56 | 3.977116e-53 |
| MsG0380016543.01 | MsG0580029522.01 | -0.843679 | 1.208263e-58 | 1.904882e-55 |
| MsG0380016544.01 | MsG0580029522.01 | -0.844588 | 6.888869e-59 | 1.117412e-55 |
| MsG0380016619.01 | MsG0580029522.01 | 0.809938 | 1.477461e-50 | 8.790131e-48 |
| MsG0380016764.01 | MsG0580029522.01 | -0.805378 | 1.374086e-49 | 7.258444e-47 |
| MsG0380017094.01 | MsG0580029522.01 | 0.819393 | 1.189399e-52 | 9.140716e-50 |
| MsG0380017143.01 | MsG0580029522.01 | -0.800561 | 1.360670e-48 | 6.355311e-46 |
| MsG0580027394.01 | MsG0580029522.01 | -0.800433 | 1.444590e-48 | 6.725676e-46 |
| MsG0580028065.01 | MsG0580029522.01 | 0.826957 | 2.039658e-54 | 1.941650e-51 |
| MsG0580029352.01 | MsG0580029522.01 | 0.827788 | 1.289112e-54 | 1.257163e-51 |
| MsG0580029522.01 | MsG0580029523.01 | 0.957961 | 7.623326e-116 | 2.730530e-110 |
| MsG0580029522.01 | MsG0580030231.01 | 0.814736 | 1.323267e-51 | 8.950795e-49 |
| MsG0580029522.01 | MsG0680030627.01 | -0.805308 | 1.421229e-49 | 7.493850e-47 |
| MsG0580029522.01 | MsG0680033744.01 | 0.800953 | 1.131566e-48 | 5.338386e-46 |
| MsG0580029522.01 | MsG0780036347.01 | 0.808968 | 2.386017e-50 | 1.383396e-47 |
| MsG0580029522.01 | MsG0780036711.01 | -0.811390 | 7.171059e-51 | 4.433228e-48 |
| MsG0580029522.01 | MsG0780038784.01 | -0.817273 | 3.591966e-52 | 2.603948e-49 |
| MsG0580029522.01 | MsG0780040826.01 | 0.812292 | 4.562955e-51 | 2.889914e-48 |
| MsG0580029522.01 | MsG0780040939.01 | 0.814345 | 1.615546e-51 | 1.081301e-48 |
| MsG0580029522.01 | MsG0880044985.01 | -0.802671 | 5.022453e-49 | 2.474873e-46 |
| MsG0580029522.01 | MsG0880045548.01 | 0.819422 | 1.171450e-52 | 9.010552e-50 |
| MsG0580029522.01 | MsG0880046791.01 | -0.803016 | 4.261737e-49 | 2.118474e-46 |
| MsG0580029522.01 | MsG0880047657.01 | -0.805942 | 1.046396e-49 | 5.608276e-47 |
| MsG0280007852.01 | MsG0580029522.01 | -0.821161 | 4.678302e-53 | 3.776870e-50 |
| MsG0280007853.01 | MsG0580029522.01 | -0.804771 | 1.840650e-49 | 9.572686e-47 |
| MsG0280008591.01 | MsG0580029522.01 | -0.812540 | 4.027567e-51 | 2.567815e-48 |
| MsG0280009775.01 | MsG0580029522.01 | -0.804559 | 2.038637e-49 | 1.054379e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029522.01.T01 | MTR_5g087790 | 97.297 | 74 | 2 | 0 | 20 | 93 | 421 | 494 | 3.45e-45 | 154 |
| MsG0580029522.01.T01 | MTR_3g078770 | 58.442 | 77 | 29 | 3 | 19 | 93 | 421 | 496 | 1.27e-21 | 88.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029522.01.T01 | AT3G20770 | 52.000 | 75 | 28 | 3 | 19 | 93 | 415 | 481 | 9.05e-14 | 65.9 |
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTTCAAGATGGTCTTCC+AGG | 0.182246 | 5:-98030473 | MsG0580029522.01.T01:CDS |
| ACCTCGGTAGCATGAAAATT+AGG | 0.199653 | 5:+98030390 | None:intergenic |
| CCACCTTCATTCGATTTAAC+TGG | 0.245739 | 5:-98030300 | MsG0580029522.01.T01:CDS |
| TTTAACTGGGTTTGGAGTTT+CGG | 0.307024 | 5:-98030286 | MsG0580029522.01.T01:CDS |
| AAGCTTTGTGGGAAAATAAC+TGG | 0.313704 | 5:+98030363 | None:intergenic |
| GCAGGCTGAACTGTAGAATT+AGG | 0.320347 | 5:+98030333 | None:intergenic |
| TCATTCGATTTAACTGGGTT+TGG | 0.382118 | 5:-98030294 | MsG0580029522.01.T01:CDS |
| TAGGTTGAACAAAGCTTTGT+GGG | 0.411860 | 5:+98030352 | None:intergenic |
| AGAAACAGTCCTGCAGGTTA+TGG | 0.412726 | 5:-98030417 | MsG0580029522.01.T01:CDS |
| CCTCGGTAGCATGAAAATTA+GGG | 0.456819 | 5:+98030391 | None:intergenic |
| CACCTTCATTCGATTTAACT+GGG | 0.462918 | 5:-98030299 | MsG0580029522.01.T01:CDS |
| GGCTATGATACACATGTTAT+AGG | 0.462962 | 5:-98030229 | None:intergenic |
| AATGATCAGCGACCTATGTC+AGG | 0.511133 | 5:-98030250 | MsG0580029522.01.T01:CDS |
| GCTCGCCTTGCCTTTCAAGA+TGG | 0.515663 | 5:-98030482 | MsG0580029522.01.T01:CDS |
| CCTTTCAAGATGGTCTTCCA+GGG | 0.519079 | 5:-98030472 | MsG0580029522.01.T01:CDS |
| ATTCAATTGATGATTGTCCC+TGG | 0.537403 | 5:+98030455 | None:intergenic |
| TTAGGTTGAACAAAGCTTTG+TGG | 0.541625 | 5:+98030351 | None:intergenic |
| GGGTTTGGAGTTTCGGAGGA+CGG | 0.542268 | 5:-98030279 | MsG0580029522.01.T01:CDS |
| AGAAACAGAAACAGTCCTGC+AGG | 0.548675 | 5:-98030423 | MsG0580029522.01.T01:CDS |
| GGACTGTTTCTGTTTCTATG+TGG | 0.550312 | 5:+98030429 | None:intergenic |
| GAAGGTGGAACTAAACTAGC+AGG | 0.551330 | 5:+98030315 | None:intergenic |
| TTAGGGTCGCCATAACCTGC+AGG | 0.552645 | 5:+98030408 | None:intergenic |
| AACCCAGTTAAATCGAATGA+AGG | 0.563631 | 5:+98030297 | None:intergenic |
| AACTGGGTTTGGAGTTTCGG+AGG | 0.578482 | 5:-98030283 | MsG0580029522.01.T01:CDS |
| CCCTGGAAGACCATCTTGAA+AGG | 0.578865 | 5:+98030472 | None:intergenic |
| TGTGTATCATAGCCTGACAT+AGG | 0.588338 | 5:+98030238 | None:intergenic |
| CCAGTTAAATCGAATGAAGG+TGG | 0.598931 | 5:+98030300 | None:intergenic |
| GCTTCGCTGTAAGCGCATTG+AGG | 0.613195 | 5:+98030503 | None:intergenic |
| GAAGACCATCTTGAAAGGCA+AGG | 0.639654 | 5:+98030477 | None:intergenic |
| GAAAATAACTGGCTTAACCT+CGG | 0.643010 | 5:+98030374 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACCCAGTTAAATCGAATGA+AGG | + | Chr5:98030449-98030468 | None:intergenic | 35.0% | |
| CACCTTCATTCGATTTAACT+GGG | - | Chr5:98030444-98030463 | MsG0580029522.01.T01:CDS | 35.0% | |
| TCATTCGATTTAACTGGGTT+TGG | - | Chr5:98030449-98030468 | MsG0580029522.01.T01:CDS | 35.0% | |
| ! | AAGCTTTGTGGGAAAATAAC+TGG | + | Chr5:98030383-98030402 | None:intergenic | 35.0% |
| ! | ATTCAATTGATGATTGTCCC+TGG | + | Chr5:98030291-98030310 | None:intergenic | 35.0% |
| ! | GAAAATAACTGGCTTAACCT+CGG | + | Chr5:98030372-98030391 | None:intergenic | 35.0% |
| ! | TAGGTTGAACAAAGCTTTGT+GGG | + | Chr5:98030394-98030413 | None:intergenic | 35.0% |
| ! | TTTAACTGGGTTTGGAGTTT+CGG | - | Chr5:98030457-98030476 | MsG0580029522.01.T01:CDS | 35.0% |
| !! | TTAGGTTGAACAAAGCTTTG+TGG | + | Chr5:98030395-98030414 | None:intergenic | 35.0% |
| ACCTCGGTAGCATGAAAATT+AGG | + | Chr5:98030356-98030375 | None:intergenic | 40.0% | |
| CCACCTTCATTCGATTTAAC+TGG | - | Chr5:98030443-98030462 | MsG0580029522.01.T01:CDS | 40.0% | |
| CCAGTTAAATCGAATGAAGG+TGG | + | Chr5:98030446-98030465 | None:intergenic | 40.0% | |
| CCTCGGTAGCATGAAAATTA+GGG | + | Chr5:98030355-98030374 | None:intergenic | 40.0% | |
| TGTGTATCATAGCCTGACAT+AGG | + | Chr5:98030508-98030527 | None:intergenic | 40.0% | |
| ! | ATAGGTCGCTGATCATTTTC+TGG | + | Chr5:98030490-98030509 | None:intergenic | 40.0% |
| !! | GGACTGTTTCTGTTTCTATG+TGG | + | Chr5:98030317-98030336 | None:intergenic | 40.0% |
| AATGATCAGCGACCTATGTC+AGG | - | Chr5:98030493-98030512 | MsG0580029522.01.T01:CDS | 45.0% | |
| AGAAACAGAAACAGTCCTGC+AGG | - | Chr5:98030320-98030339 | MsG0580029522.01.T01:CDS | 45.0% | |
| AGAAACAGTCCTGCAGGTTA+TGG | - | Chr5:98030326-98030345 | MsG0580029522.01.T01:CDS | 45.0% | |
| CCTTTCAAGATGGTCTTCCA+GGG | - | Chr5:98030271-98030290 | MsG0580029522.01.T01:CDS | 45.0% | |
| GAAGACCATCTTGAAAGGCA+AGG | + | Chr5:98030269-98030288 | None:intergenic | 45.0% | |
| GAAGGTGGAACTAAACTAGC+AGG | + | Chr5:98030431-98030450 | None:intergenic | 45.0% | |
| GCAGGCTGAACTGTAGAATT+AGG | + | Chr5:98030413-98030432 | None:intergenic | 45.0% | |
| ! | CCCTAATTTTCATGCTACCG+AGG | - | Chr5:98030352-98030371 | MsG0580029522.01.T01:CDS | 45.0% |
| CCCTGGAAGACCATCTTGAA+AGG | + | Chr5:98030274-98030293 | None:intergenic | 50.0% | |
| GCCTTTCAAGATGGTCTTCC+AGG | - | Chr5:98030270-98030289 | MsG0580029522.01.T01:CDS | 50.0% | |
| ! | AACTGGGTTTGGAGTTTCGG+AGG | - | Chr5:98030460-98030479 | MsG0580029522.01.T01:CDS | 50.0% |
| GCTCGCCTTGCCTTTCAAGA+TGG | - | Chr5:98030261-98030280 | MsG0580029522.01.T01:CDS | 55.0% | |
| GCTTCGCTGTAAGCGCATTG+AGG | + | Chr5:98030243-98030262 | None:intergenic | 55.0% | |
| TTAGGGTCGCCATAACCTGC+AGG | + | Chr5:98030338-98030357 | None:intergenic | 55.0% | |
| ! | GGGTTTGGAGTTTCGGAGGA+CGG | - | Chr5:98030464-98030483 | MsG0580029522.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 98030230 | 98030535 | 98030230 | ID=MsG0580029522.01;Name=MsG0580029522.01 |
| Chr5 | mRNA | 98030230 | 98030535 | 98030230 | ID=MsG0580029522.01.T01;Parent=MsG0580029522.01;Name=MsG0580029522.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|101 |
| Chr5 | exon | 98030230 | 98030535 | 98030230 | ID=MsG0580029522.01.T01:exon:25399;Parent=MsG0580029522.01.T01 |
| Chr5 | CDS | 98030230 | 98030535 | 98030230 | ID=MsG0580029522.01.T01:cds;Parent=MsG0580029522.01.T01 |
| Gene Sequence |
| Protein sequence |
