AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040826.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040826.01.T01 MTR_7g099210 69.463 298 15 4 24 252 188 478 2.27e-124 371
MsG0780040826.01.T01 MTR_1g069355 40.876 274 112 7 10 255 205 456 1.23e-45 162
MsG0780040826.01.T01 MTR_1g069355 40.876 274 112 7 10 255 205 456 1.62e-45 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040826.01.T01 AT1G04140 30.116 259 128 9 24 250 224 461 8.55e-18 83.2
MsG0780040826.01.T01 AT1G04140 30.116 259 128 9 24 250 224 461 8.62e-18 83.2
MsG0780040826.01.T01 AT5G43930 48.750 80 36 1 10 89 237 311 1.10e-15 76.6
MsG0780040826.01.T01 AT5G43930 50.000 80 33 1 10 89 213 285 1.12e-15 76.6
MsG0780040826.01.T01 AT5G43930 50.000 80 33 1 10 89 213 285 1.12e-15 76.6
MsG0780040826.01.T01 AT5G43930 50.000 80 33 1 10 89 213 285 1.12e-15 76.6

Find 60 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCCTCTTATATTGTGTTC+TGG 0.308760 7:-83009024 None:intergenic
TTCTCTAATGGAATGGGAAT+TGG 0.338125 7:-83008378 MsG0780040826.01.T01:CDS
GTGCATACTGCTTTCTCTAA+TGG 0.347834 7:-83008390 MsG0780040826.01.T01:CDS
ACCTCGGCCGTTAAAAGATA+TGG 0.356344 7:+83008880 None:intergenic
GCTAGTCATGACGCTGGTTC+AGG 0.361696 7:-83008552 MsG0780040826.01.T01:CDS
TTGACTTCCTTCAGCCGGTC+TGG 0.395921 7:+83008314 None:intergenic
TTTCTACAACAGGATGATGA+TGG 0.458593 7:-83008148 MsG0780040826.01.T01:intron
CTGTGTTGACTTCCTTCAGC+CGG 0.460994 7:+83008309 None:intergenic
ATGCCATCTTCTGCAATGTC+TGG 0.461031 7:-83008001 MsG0780040826.01.T01:CDS
ATATCGTGCAGCTGTACATA+TGG 0.461205 7:-83008998 MsG0780040826.01.T01:CDS
TACTGCTTTCTCTAATGGAA+TGG 0.472180 7:-83008385 MsG0780040826.01.T01:CDS
CCCGTGTGAGGTAGCATTGA+GGG 0.475913 7:+83008106 None:intergenic
CCCCTCAATGCTACCTCACA+CGG 0.479076 7:-83008107 MsG0780040826.01.T01:CDS
ACTGCTTTCTCTAATGGAAT+GGG 0.479934 7:-83008384 MsG0780040826.01.T01:CDS
CTGCCAGACATTGCAGAAGA+TGG 0.486488 7:+83007998 None:intergenic
ACAGATTAGTTCTAGTGTAA+TGG 0.489225 7:-83008059 MsG0780040826.01.T01:CDS
AACCTGATCTACAGAAGATC+GGG 0.499387 7:+83007965 None:intergenic
ATGACAGAGGCGACGTCCAT+TGG 0.504441 7:-83008725 MsG0780040826.01.T01:CDS
GTCTCCCATGGATACAGTCT+TGG 0.511829 7:-83008451 MsG0780040826.01.T01:CDS
GATGATGGGTCAAGCCAAGC+TGG 0.524347 7:-83008133 MsG0780040826.01.T01:CDS
CAACCTCACAATGGACGGTA+TGG 0.524573 7:-83008349 MsG0780040826.01.T01:CDS
AAAAGATATGGCGCAGCATG+AGG 0.527214 7:+83008892 None:intergenic
TAAGAGAACTTGCATCGGTA+GGG 0.528435 7:+83008283 None:intergenic
AATCTCCAAGACTGTATCCA+TGG 0.530387 7:+83008446 None:intergenic
TTAAGAGAACTTGCATCGGT+AGG 0.532461 7:+83008282 None:intergenic
AAACCTGATCTACAGAAGAT+CGG 0.549118 7:+83007964 None:intergenic
TGCCGTGGCTCAGTTTCAAG+CGG 0.552360 7:-83008508 MsG0780040826.01.T01:CDS
TTCTACAACAGGATGATGAT+GGG 0.561989 7:-83008147 MsG0780040826.01.T01:intron
ATCGGCAACCTCACAATGGA+CGG 0.577256 7:-83008354 MsG0780040826.01.T01:CDS
TAAAGACAAGGCGATCTCTA+CGG 0.578661 7:-83008929 MsG0780040826.01.T01:CDS
ATATGGCACTACGACAAGAA+AGG 0.584515 7:-83008981 MsG0780040826.01.T01:CDS
GGACGTCGCCTCTGTCATTG+AGG 0.588258 7:+83008730 None:intergenic
GATGAAACCAGACCGGCTGA+AGG 0.588340 7:-83008321 MsG0780040826.01.T01:CDS
ACCCGTGTGAGGTAGCATTG+AGG 0.592212 7:+83008105 None:intergenic
GCATTGAGGGGAGACCAGCT+TGG 0.596774 7:+83008119 None:intergenic
AATGGAATGGGAATTGGCAT+CGG 0.601027 7:-83008372 MsG0780040826.01.T01:CDS
ATTAGAGAAAGCAGTATGCA+CGG 0.601449 7:+83008392 None:intergenic
AGGATATTGTAAGTAACCAA+TGG 0.603398 7:+83008709 None:intergenic
ATCTCCAAGACTGTATCCAT+GGG 0.604350 7:+83008447 None:intergenic
GTACCAGCTTGAGAGTTAGT+CGG 0.607254 7:+83008423 None:intergenic
TGCTACATCAGCATTAGAAG+TGG 0.609631 7:+83008024 None:intergenic
TGATACTATAGTGTCTCCCA+TGG 0.611348 7:-83008463 MsG0780040826.01.T01:CDS
TTAGAGAAAGCAGTATGCAC+GGG 0.618198 7:+83008393 None:intergenic
GCTACATCAGCATTAGAAGT+GGG 0.622520 7:+83008025 None:intergenic
TACATTAAGAGAACTTGCAT+CGG 0.624258 7:+83008278 None:intergenic
GACAAAGTAACATGTTCTGT+AGG 0.624906 7:+83008656 None:intergenic
AAAGATATGGCGCAGCATGA+GGG 0.625734 7:+83008893 None:intergenic
ATTCCGACTAACTCTCAAGC+TGG 0.626131 7:-83008426 MsG0780040826.01.T01:CDS
TCTCCATACCGTCCATTGTG+AGG 0.627723 7:+83008346 None:intergenic
ACAAATTGAGTCTTCTGCCG+TGG 0.631489 7:-83008523 MsG0780040826.01.T01:CDS
CCCTCAATGCTACCTCACAC+GGG 0.637598 7:-83008106 MsG0780040826.01.T01:CDS
TGGCATCGGCAACCTCACAA+TGG 0.648272 7:-83008358 MsG0780040826.01.T01:CDS
GTCCGCTTGAAACTGAGCCA+CGG 0.655549 7:+83008506 None:intergenic
CCGTGTGAGGTAGCATTGAG+GGG 0.655824 7:+83008107 None:intergenic
ATTACACTAGAACTAATCTG+TGG 0.661265 7:+83008061 None:intergenic
GGAGACTGATGAAACCAGAC+CGG 0.663373 7:-83008328 MsG0780040826.01.T01:CDS
TTCAGATTCCTCAATGACAG+AGG 0.666751 7:-83008738 MsG0780040826.01.T01:CDS
TCACGACTAACACCCGTGTG+AGG 0.675774 7:+83008094 None:intergenic
CAGCATGCTAGTCATGACGC+TGG 0.681322 7:-83008558 MsG0780040826.01.T01:CDS
AAACACAAGTTCATTCACCT+CGG 0.683452 7:+83008864 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTCTTTAACACAAAAATT+GGG + Chr7:83008045-83008064 None:intergenic 20.0%
! AATTAACATAGTGAATAGCA+TGG + Chr7:83008163-83008182 None:intergenic 25.0%
! ATTAACATAGTGAATAGCAT+GGG + Chr7:83008162-83008181 None:intergenic 25.0%
! CTTGTCTTTAACACAAAAAT+TGG + Chr7:83008046-83008065 None:intergenic 25.0%
! TGTCTTTAACACAAAAATTG+GGG + Chr7:83008044-83008063 None:intergenic 25.0%
! TTCTCTTAATGTATGTTACA+TGG - Chr7:83008717-83008736 MsG0780040826.01.T01:CDS 25.0%
!! CAATTTTTGTGTTAAAGACA+AGG - Chr7:83008044-83008063 MsG0780040826.01.T01:CDS 25.0%
AGGATATTGTAAGTAACCAA+TGG + Chr7:83008279-83008298 None:intergenic 30.0%
ATTACACTAGAACTAATCTG+TGG + Chr7:83008927-83008946 None:intergenic 30.0%
TACATTAAGAGAACTTGCAT+CGG + Chr7:83008710-83008729 None:intergenic 30.0%
!! ACAGATTAGTTCTAGTGTAA+TGG - Chr7:83008926-83008945 MsG0780040826.01.T01:CDS 30.0%
AAACACAAGTTCATTCACCT+CGG + Chr7:83008124-83008143 None:intergenic 35.0%
ACATTTGTGACAAAAACAGC+AGG + Chr7:83008305-83008324 None:intergenic 35.0%
ACTGCTTTCTCTAATGGAAT+GGG - Chr7:83008601-83008620 MsG0780040826.01.T01:intron 35.0%
ATTAGAGAAAGCAGTATGCA+CGG + Chr7:83008596-83008615 None:intergenic 35.0%
GACAAAGTAACATGTTCTGT+AGG + Chr7:83008332-83008351 None:intergenic 35.0%
TAACACAAAAATTGGGGAGT+AGG + Chr7:83008038-83008057 None:intergenic 35.0%
TACTGCTTTCTCTAATGGAA+TGG - Chr7:83008600-83008619 MsG0780040826.01.T01:intron 35.0%
TCATCATCCTGTTGTAGAAA+AGG + Chr7:83008837-83008856 None:intergenic 35.0%
TTCTACAACAGGATGATGAT+GGG - Chr7:83008838-83008857 MsG0780040826.01.T01:intron 35.0%
TTCTCTAATGGAATGGGAAT+TGG - Chr7:83008607-83008626 MsG0780040826.01.T01:intron 35.0%
TTTCTACAACAGGATGATGA+TGG - Chr7:83008837-83008856 MsG0780040826.01.T01:intron 35.0%
! GGAATTACCTTTTCTACAAC+AGG - Chr7:83008827-83008846 MsG0780040826.01.T01:intron 35.0%
! GGTGAATGAACTTGTGTTTT+TGG - Chr7:83008125-83008144 MsG0780040826.01.T01:CDS 35.0%
AAAGGCAATGACACATTAGG+TGG + Chr7:83008362-83008381 None:intergenic 40.0%
AAAGGTAATTCCCATCCACT+TGG + Chr7:83008819-83008838 None:intergenic 40.0%
AAGGTAATTCCCATCCACTT+GGG + Chr7:83008818-83008837 None:intergenic 40.0%
AATCTCCAAGACTGTATCCA+TGG + Chr7:83008542-83008561 None:intergenic 40.0%
AATGGAATGGGAATTGGCAT+CGG - Chr7:83008613-83008632 MsG0780040826.01.T01:intron 40.0%
ATATCGTGCAGCTGTACATA+TGG - Chr7:83007987-83008006 MsG0780040826.01.T01:CDS 40.0%
ATATGGCACTACGACAAGAA+AGG - Chr7:83008004-83008023 MsG0780040826.01.T01:CDS 40.0%
ATCTCCAAGACTGTATCCAT+GGG + Chr7:83008541-83008560 None:intergenic 40.0%
CTAGACAAACCGCAAATCAT+GGG - Chr7:83008745-83008764 MsG0780040826.01.T01:CDS 40.0%
CTCGACTCATCAACAGAATA+CGG + Chr7:83008397-83008416 None:intergenic 40.0%
GAGAAAGGCAATGACACATT+AGG + Chr7:83008365-83008384 None:intergenic 40.0%
GCTACATCAGCATTAGAAGT+GGG + Chr7:83008963-83008982 None:intergenic 40.0%
GGGTCTCTTGAAGAAACAAA+CGG + Chr7:83008798-83008817 None:intergenic 40.0%
GTGCATACTGCTTTCTCTAA+TGG - Chr7:83008595-83008614 MsG0780040826.01.T01:intron 40.0%
TAAAGACAAGGCGATCTCTA+CGG - Chr7:83008056-83008075 MsG0780040826.01.T01:CDS 40.0%
TAGACAAACCGCAAATCATG+GGG - Chr7:83008746-83008765 MsG0780040826.01.T01:CDS 40.0%
TCTAGACAAACCGCAAATCA+TGG - Chr7:83008744-83008763 MsG0780040826.01.T01:CDS 40.0%
TGATACTATAGTGTCTCCCA+TGG - Chr7:83008522-83008541 MsG0780040826.01.T01:CDS 40.0%
TGCTACATCAGCATTAGAAG+TGG + Chr7:83008964-83008983 None:intergenic 40.0%
TTAGAGAAAGCAGTATGCAC+GGG + Chr7:83008595-83008614 None:intergenic 40.0%
TTCAGATTCCTCAATGACAG+AGG - Chr7:83008247-83008266 MsG0780040826.01.T01:intron 40.0%
TTTGTGACAAAAACAGCAGG+TGG + Chr7:83008302-83008321 None:intergenic 40.0%
! TAAGAGAACTTGCATCGGTA+GGG + Chr7:83008705-83008724 None:intergenic 40.0%
! TGCTGCGCCATATCTTTTAA+CGG - Chr7:83008098-83008117 MsG0780040826.01.T01:CDS 40.0%
! TTAAGAGAACTTGCATCGGT+AGG + Chr7:83008706-83008725 None:intergenic 40.0%
AAAAGATATGGCGCAGCATG+AGG + Chr7:83008096-83008115 None:intergenic 45.0%
AAAGATATGGCGCAGCATGA+GGG + Chr7:83008095-83008114 None:intergenic 45.0%
AAATCATGGGGTTCATCCAG+AGG - Chr7:83008758-83008777 MsG0780040826.01.T01:CDS 45.0%
ACAAATTGAGTCTTCTGCCG+TGG - Chr7:83008462-83008481 MsG0780040826.01.T01:CDS 45.0%
ACCTCGGCCGTTAAAAGATA+TGG + Chr7:83008108-83008127 None:intergenic 45.0%
ATGCCATCTTCTGCAATGTC+TGG - Chr7:83008984-83009003 MsG0780040826.01.T01:CDS 45.0%
ATTCCGACTAACTCTCAAGC+TGG - Chr7:83008559-83008578 MsG0780040826.01.T01:intron 45.0%
GACTCATCAACAGAATACGG+AGG + Chr7:83008394-83008413 None:intergenic 45.0%
GGATGAAATTGACCATCCTC+TGG + Chr7:83008777-83008796 None:intergenic 45.0%
GTACCAGCTTGAGAGTTAGT+CGG + Chr7:83008565-83008584 None:intergenic 45.0%
GTTTCTTCAAGAGACCCAAG+TGG - Chr7:83008801-83008820 MsG0780040826.01.T01:intron 45.0%
TGGATGAACCCCATGATTTG+CGG + Chr7:83008757-83008776 None:intergenic 45.0%
TTCAAGAGACCCAAGTGGAT+GGG - Chr7:83008806-83008825 MsG0780040826.01.T01:intron 45.0%
!!! TTTTTATTTTATTTTTCTTT+TGG - Chr7:83008182-83008201 MsG0780040826.01.T01:intron 5.0%
ACATGCTTGCTTTGATGCGC+AGG - Chr7:83008208-83008227 MsG0780040826.01.T01:intron 50.0%
ATCGGCAACCTCACAATGGA+CGG - Chr7:83008631-83008650 MsG0780040826.01.T01:intron 50.0%
CAACCTCACAATGGACGGTA+TGG - Chr7:83008636-83008655 MsG0780040826.01.T01:intron 50.0%
CTGCCAGACATTGCAGAAGA+TGG + Chr7:83008990-83009009 None:intergenic 50.0%
CTTCAAGAGACCCAAGTGGA+TGG - Chr7:83008805-83008824 MsG0780040826.01.T01:intron 50.0%
GGAGACTGATGAAACCAGAC+CGG - Chr7:83008657-83008676 MsG0780040826.01.T01:intron 50.0%
GTCTCCCATGGATACAGTCT+TGG - Chr7:83008534-83008553 MsG0780040826.01.T01:CDS 50.0%
GTGACAAAAACAGCAGGTGG+AGG + Chr7:83008299-83008318 None:intergenic 50.0%
TCTCCATACCGTCCATTGTG+AGG + Chr7:83008642-83008661 None:intergenic 50.0%
! GCCATATCTTTTAACGGCCG+AGG - Chr7:83008104-83008123 MsG0780040826.01.T01:CDS 50.0%
!! CTGTGTTGACTTCCTTCAGC+CGG + Chr7:83008679-83008698 None:intergenic 50.0%
ACCCGTGTGAGGTAGCATTG+AGG + Chr7:83008883-83008902 None:intergenic 55.0%
ATGACAGAGGCGACGTCCAT+TGG - Chr7:83008260-83008279 MsG0780040826.01.T01:intron 55.0%
CAGCATGCTAGTCATGACGC+TGG - Chr7:83008427-83008446 MsG0780040826.01.T01:CDS 55.0%
CATGGGGTTCATCCAGAGGA+TGG - Chr7:83008762-83008781 MsG0780040826.01.T01:intron 55.0%
CCCCTCAATGCTACCTCACA+CGG - Chr7:83008878-83008897 MsG0780040826.01.T01:CDS 55.0%
CCCTCAATGCTACCTCACAC+GGG - Chr7:83008879-83008898 MsG0780040826.01.T01:CDS 55.0%
GATGAAACCAGACCGGCTGA+AGG - Chr7:83008664-83008683 MsG0780040826.01.T01:intron 55.0%
GTCCGCTTGAAACTGAGCCA+CGG + Chr7:83008482-83008501 None:intergenic 55.0%
TCACGACTAACACCCGTGTG+AGG + Chr7:83008894-83008913 None:intergenic 55.0%
TGCCGTGGCTCAGTTTCAAG+CGG - Chr7:83008477-83008496 MsG0780040826.01.T01:CDS 55.0%
TTGACTTCCTTCAGCCGGTC+TGG + Chr7:83008674-83008693 None:intergenic 55.0%
! ATACGGAGGCACGAGGAGAA+AGG + Chr7:83008380-83008399 None:intergenic 55.0%
! CAACAGAATACGGAGGCACG+AGG + Chr7:83008387-83008406 None:intergenic 55.0%
! CCCGTGTGAGGTAGCATTGA+GGG + Chr7:83008882-83008901 None:intergenic 55.0%
! CCGTGTGAGGTAGCATTGAG+GGG + Chr7:83008881-83008900 None:intergenic 55.0%
!! GATGATGGGTCAAGCCAAGC+TGG - Chr7:83008852-83008871 MsG0780040826.01.T01:intron 55.0%
!! GCTAGTCATGACGCTGGTTC+AGG - Chr7:83008433-83008452 MsG0780040826.01.T01:CDS 55.0%
!! TGGCATCGGCAACCTCACAA+TGG - Chr7:83008627-83008646 MsG0780040826.01.T01:intron 55.0%
GCATTGAGGGGAGACCAGCT+TGG + Chr7:83008869-83008888 None:intergenic 60.0%
GGACGTCGCCTCTGTCATTG+AGG + Chr7:83008258-83008277 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 83007973 83009034 83007973 ID=MsG0780040826.01;Name=MsG0780040826.01
Chr7 mRNA 83007973 83009034 83007973 ID=MsG0780040826.01.T01;Parent=MsG0780040826.01;Name=MsG0780040826.01.T01;_AED=0.45;_eAED=0.47;_QI=0|0|0|1|0.66|0.75|4|0|255
Chr7 exon 83008861 83009034 83008861 ID=MsG0780040826.01.T01:exon:38568;Parent=MsG0780040826.01.T01
Chr7 exon 83008667 83008777 83008667 ID=MsG0780040826.01.T01:exon:38567;Parent=MsG0780040826.01.T01
Chr7 exon 83008281 83008577 83008281 ID=MsG0780040826.01.T01:exon:38566;Parent=MsG0780040826.01.T01
Chr7 exon 83007973 83008158 83007973 ID=MsG0780040826.01.T01:exon:38565;Parent=MsG0780040826.01.T01
Chr7 CDS 83008861 83009034 83008861 ID=MsG0780040826.01.T01:cds;Parent=MsG0780040826.01.T01
Chr7 CDS 83008667 83008777 83008667 ID=MsG0780040826.01.T01:cds;Parent=MsG0780040826.01.T01
Chr7 CDS 83008281 83008577 83008281 ID=MsG0780040826.01.T01:cds;Parent=MsG0780040826.01.T01
Chr7 CDS 83007973 83008158 83007973 ID=MsG0780040826.01.T01:cds;Parent=MsG0780040826.01.T01
Gene Sequence

>MsG0780040826.01.T01

TTGTGTTCTGGAATATATCGTGCAGCTGTACATATGGCACTACGACAAGAAAGGCGAAGCATCCTACTCCCCAATTTTTGTGTTAAAGACAAGGCGATCTCTACGGCTGTTCATTTTCACCCTCATGCTGCGCCATATCTTTTAACGGCCGAGGTGAATGAACTTGTGTTTTTGGTCAACGATCTTGACTCTTCAGATTCCTCAATGACAGAGGCGACGTCCATTGGTTACTTACAATATCCTCCACCTGCTGTTTTTGTCACAAATGTTCATCCTACAGAACATCATGCTAGTCATGACGCTGGTTCAGGTAGAATACAAATTGAGTCTTCTGCCGTGGCTCAGTTTCAAGCGGACACAAATTCAACAGAACAACATGATACTATAGTGTCTCCCATGGATACAGTCTTGGAGATTCCGACTAACTCTCAAGCTGGTACAGAATATCCCGTGCATACTGCTTTCTCTAATGGAATGGGAATTGGCATCGGCAACCTCACAATGGACGGTATGGAGACTGATGAAACCAGACCGGCTGAAGGAAGTCAACACAGAAACCCTACCGATGCAAGTTCTCTTAATGATGATGATGGGTCAAGCCAAGCTGGTCTCCCCTCAATGCTACCTCACACGGGTGTTAGTCGTGACACTCTAGCTCCACAGATTAGTTCTAGTGTAATGGCTAATACTCTTCCCACTTCTAATGCTGATGTAGCAATGCCATCTTCTGCAATGTCTGGCAGCATTAACATACCCGATCTTCTGTAG

Protein sequence

>MsG0780040826.01.T01

LCSGIYRAAVHMALRQERRSILLPNFCVKDKAISTAVHFHPHAAPYLLTAEVNELVFLVNDLDSSDSSMTEATSIGYLQYPPPAVFVTNVHPTEHHASHDAGSGRIQIESSAVAQFQADTNSTEQHDTIVSPMDTVLEIPTNSQAGTEYPVHTAFSNGMGIGIGNLTMDGMETDETRPAEGSQHRNPTDASSLNDDDGSSQAGLPSMLPHTGVSRDTLAPQISSSVMANTLPTSNADVAMPSSAMSGSINIPDLL*