Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032355.01.T01 | XP_024636184.2 | 71.852 | 135 | 23 | 1 | 1 | 120 | 184 | 318 | 1.20E-62 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032355.01.T01 | A0A072TG10 | 70.677 | 133 | 24 | 1 | 1 | 118 | 101 | 233 | 2.62e-59 | 193 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0680032355.01 | 0.807159 | 5.792220e-50 | 3.202799e-47 |
| MsG0180000470.01 | MsG0680032355.01 | 0.838804 | 2.312672e-57 | 3.132326e-54 |
| MsG0180001063.01 | MsG0680032355.01 | 0.827043 | 1.945243e-54 | 1.856344e-51 |
| MsG0180002282.01 | MsG0680032355.01 | 0.801509 | 8.705426e-49 | 4.164925e-46 |
| MsG0180002488.01 | MsG0680032355.01 | 0.809633 | 1.718502e-50 | 1.014301e-47 |
| MsG0180002842.01 | MsG0680032355.01 | 0.800026 | 1.747723e-48 | 8.053242e-46 |
| MsG0180004275.01 | MsG0680032355.01 | 0.807952 | 3.930686e-50 | 2.218862e-47 |
| MsG0280006324.01 | MsG0680032355.01 | 0.802005 | 6.887500e-49 | 3.336777e-46 |
| MsG0280006325.01 | MsG0680032355.01 | 0.803697 | 3.079804e-49 | 1.558021e-46 |
| MsG0280011032.01 | MsG0680032355.01 | 0.802612 | 5.165477e-49 | 2.541555e-46 |
| MsG0380011609.01 | MsG0680032355.01 | 0.815699 | 8.088622e-52 | 5.616758e-49 |
| MsG0380012007.01 | MsG0680032355.01 | 0.805717 | 1.166266e-49 | 6.215090e-47 |
| MsG0380012330.01 | MsG0680032355.01 | 0.834484 | 2.918718e-56 | 3.468634e-53 |
| MsG0380012361.01 | MsG0680032355.01 | 0.810198 | 1.298364e-50 | 7.776890e-48 |
| MsG0380014251.01 | MsG0680032355.01 | 0.816449 | 5.499654e-52 | 3.897815e-49 |
| MsG0680030880.01 | MsG0680032355.01 | 0.806052 | 9.919055e-50 | 5.331135e-47 |
| MsG0680030882.01 | MsG0680032355.01 | 0.810171 | 1.316140e-50 | 7.877987e-48 |
| MsG0680031379.01 | MsG0680032355.01 | 0.813150 | 2.961284e-51 | 1.918628e-48 |
| MsG0680032157.01 | MsG0680032355.01 | 0.818106 | 2.330701e-52 | 1.728668e-49 |
| MsG0680032158.01 | MsG0680032355.01 | 0.809291 | 2.034733e-50 | 1.190011e-47 |
| MsG0680032355.01 | MsG0680034565.01 | 0.802537 | 5.352337e-49 | 2.628450e-46 |
| MsG0680032355.01 | MsG0680035444.01 | 0.813311 | 2.729285e-51 | 1.776202e-48 |
| MsG0680032355.01 | MsG0780036080.01 | 0.820368 | 7.118149e-53 | 5.621405e-50 |
| MsG0680032355.01 | MsG0780039631.01 | 0.816842 | 4.489659e-52 | 3.216716e-49 |
| MsG0680032355.01 | MsG0880042161.01 | 0.800220 | 1.596306e-48 | 7.391857e-46 |
| MsG0680032355.01 | MsG0880042966.01 | 0.804818 | 1.799481e-49 | 9.370415e-47 |
| MsG0680032355.01 | MsG0880043812.01 | 0.813325 | 2.710566e-51 | 1.764691e-48 |
| MsG0480021276.01 | MsG0680032355.01 | 0.804590 | 2.007702e-49 | 1.039246e-46 |
| MsG0480021536.01 | MsG0680032355.01 | 0.819428 | 1.167642e-52 | 8.982811e-50 |
| MsG0480022259.01 | MsG0680032355.01 | 0.841952 | 3.476972e-58 | 5.191624e-55 |
| MsG0480022262.01 | MsG0680032355.01 | 0.835335 | 1.782072e-56 | 2.172692e-53 |
| MsG0580024552.01 | MsG0680032355.01 | 0.806037 | 9.994148e-50 | 5.369381e-47 |
| MsG0580025638.01 | MsG0680032355.01 | 0.820079 | 8.290950e-53 | 6.495850e-50 |
| MsG0380017592.01 | MsG0680032355.01 | 0.801933 | 7.126679e-49 | 3.446332e-46 |
| MsG0480018236.01 | MsG0680032355.01 | 0.818339 | 2.064360e-52 | 1.541109e-49 |
| MsG0480018372.01 | MsG0680032355.01 | 0.830437 | 2.938311e-55 | 3.095567e-52 |
| MsG0580026122.01 | MsG0680032355.01 | 0.804846 | 1.775788e-49 | 9.253402e-47 |
| MsG0580026342.01 | MsG0680032355.01 | 0.803748 | 3.006530e-49 | 1.522930e-46 |
| MsG0580027018.01 | MsG0680032355.01 | 0.803472 | 3.429743e-49 | 1.725032e-46 |
| MsG0280008894.01 | MsG0680032355.01 | 0.815156 | 1.067898e-51 | 7.305614e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032355.01.T01 | MTR_0462s0020 | 70.677 | 133 | 24 | 1 | 1 | 118 | 101 | 233 | 6.65e-63 | 193 |
| MsG0680032355.01.T01 | MTR_2g092960 | 47.727 | 132 | 53 | 2 | 1 | 117 | 1026 | 1156 | 1.05e-34 | 126 |
| MsG0680032355.01.T01 | MTR_8g017000 | 44.030 | 134 | 59 | 2 | 1 | 118 | 494 | 627 | 8.28e-33 | 120 |
| MsG0680032355.01.T01 | MTR_2g037270 | 43.284 | 134 | 60 | 2 | 1 | 118 | 494 | 627 | 4.41e-32 | 119 |
| MsG0680032355.01.T01 | MTR_4g048180 | 55.172 | 116 | 29 | 5 | 1 | 116 | 117 | 209 | 7.66e-31 | 110 |
| MsG0680032355.01.T01 | MTR_3g011560 | 42.742 | 124 | 55 | 2 | 1 | 108 | 494 | 617 | 8.97e-30 | 112 |
| MsG0680032355.01.T01 | MTR_2g023630 | 29.412 | 119 | 64 | 1 | 1 | 119 | 193 | 291 | 6.87e-15 | 69.3 |
| MsG0680032355.01.T01 | MTR_5g077900 | 31.852 | 135 | 71 | 3 | 2 | 117 | 31 | 163 | 3.15e-12 | 61.2 |
| MsG0680032355.01.T01 | MTR_7g046380 | 32.283 | 127 | 65 | 3 | 10 | 117 | 221 | 345 | 6.37e-12 | 61.2 |
| MsG0680032355.01.T01 | MTR_0002s1270 | 31.852 | 135 | 71 | 3 | 2 | 117 | 205 | 337 | 9.24e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTACACGATTCTACGAATA+TGG | 0.218238 | 6:-39217323 | MsG0680032355.01.T01:CDS |
| GGAACGCCTATGTACTCAAA+TGG | 0.394432 | 6:-39217125 | MsG0680032355.01.T01:CDS |
| TATGAGACTGAGAACAATCT+TGG | 0.418565 | 6:+39217047 | None:intergenic |
| ATGGGAAATAATGATGTGTA+TGG | 0.441652 | 6:-39217106 | MsG0680032355.01.T01:CDS |
| TTTACCCTCGAACAGCTTTG+CGG | 0.442225 | 6:+39217021 | None:intergenic |
| AATTATTGTAGTGCTATTGA+AGG | 0.459831 | 6:-39216707 | MsG0680032355.01.T01:intron |
| ATGTTCGATGGGGTGGAAGC+CGG | 0.483605 | 6:-39216873 | MsG0680032355.01.T01:CDS |
| GAAAGCCGAGGAAGCCATCA+TGG | 0.490617 | 6:-39216661 | MsG0680032355.01.T01:CDS |
| TTTCCAAATGTAGTCGCCAT+TGG | 0.490741 | 6:-39216940 | MsG0680032355.01.T01:intron |
| GAACGCCTATGTACTCAAAT+GGG | 0.497694 | 6:-39217124 | MsG0680032355.01.T01:CDS |
| CTACACGATTCTACGAATAT+GGG | 0.518377 | 6:-39217322 | MsG0680032355.01.T01:CDS |
| TATTTCCCATTTGAGTACAT+AGG | 0.523178 | 6:+39217119 | None:intergenic |
| ATTATTGTAGTGCTATTGAA+GGG | 0.549725 | 6:-39216706 | MsG0680032355.01.T01:intron |
| GCTTCCACCCCATCGAACAT+AGG | 0.550012 | 6:+39216876 | None:intergenic |
| GTATCCCATGATGGCTTCCT+CGG | 0.560438 | 6:+39216656 | None:intergenic |
| CAATAATGAGCAATTGCCAA+TGG | 0.614170 | 6:+39216924 | None:intergenic |
| AAAGCCGAGGAAGCCATCAT+GGG | 0.623225 | 6:-39216660 | MsG0680032355.01.T01:CDS |
| ACTGTGGGAGTATCCCATGA+TGG | 0.628824 | 6:+39216647 | None:intergenic |
| TGAGCGGTTTGAGAAAGCCG+AGG | 0.628929 | 6:-39216673 | MsG0680032355.01.T01:CDS |
| GAAGGGTATATTAAGTTGAG+CGG | 0.631270 | 6:-39216689 | MsG0680032355.01.T01:CDS |
| CACGATTCTACGAATATGGG+TGG | 0.634081 | 6:-39217319 | MsG0680032355.01.T01:CDS |
| TGGGAAATAATGATGTGTAT+GGG | 0.645102 | 6:-39217105 | MsG0680032355.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAATTCTTTCCAATATAT+AGG | + | Chr6:39217022-39217041 | None:intergenic | 15.0% |
| !!! | AAAACTACATTTTGTTTTTT+TGG | + | Chr6:39217098-39217117 | None:intergenic | 15.0% |
| !!! | GAGATTTTTTTTAATATTGT+AGG | - | Chr6:39216841-39216860 | MsG0680032355.01.T01:intron | 15.0% |
| !!! | TTTTTAATATTGTAGGTATA+TGG | - | Chr6:39216848-39216867 | MsG0680032355.01.T01:CDS | 15.0% |
| !! | TCTTGTAAAAAAATCAAGTT+GGG | + | Chr6:39217247-39217266 | None:intergenic | 20.0% |
| ! | ATTCTTTCCAATATATAGGT+AGG | + | Chr6:39217018-39217037 | None:intergenic | 25.0% |
| ! | GTCTTGTAAAAAAATCAAGT+TGG | + | Chr6:39217248-39217267 | None:intergenic | 25.0% |
| ! | TTCTTTCCAATATATAGGTA+GGG | + | Chr6:39217017-39217036 | None:intergenic | 25.0% |
| !! | AAAAACGTGTTTCTGATTTA+TGG | - | Chr6:39216782-39216801 | MsG0680032355.01.T01:intron | 25.0% |
| !! | AATTATTGTAGTGCTATTGA+AGG | - | Chr6:39217287-39217306 | MsG0680032355.01.T01:intron | 25.0% |
| !! | ATTATTGTAGTGCTATTGAA+GGG | - | Chr6:39217288-39217307 | MsG0680032355.01.T01:intron | 25.0% |
| ATGGGAAATAATGATGTGTA+TGG | - | Chr6:39216888-39216907 | MsG0680032355.01.T01:CDS | 30.0% | |
| TATTTCCCATTTGAGTACAT+AGG | + | Chr6:39216878-39216897 | None:intergenic | 30.0% | |
| TGGGAAATAATGATGTGTAT+GGG | - | Chr6:39216889-39216908 | MsG0680032355.01.T01:CDS | 30.0% | |
| ! | TTTTCTGCGTGAATGAAAAA+TGG | + | Chr6:39216924-39216943 | None:intergenic | 30.0% |
| !! | ATGTGCTCGATATTTTTATC+CGG | + | Chr6:39217143-39217162 | None:intergenic | 30.0% |
| CAATAATGAGCAATTGCCAA+TGG | + | Chr6:39217073-39217092 | None:intergenic | 35.0% | |
| CTACACGATTCTACGAATAT+GGG | - | Chr6:39216672-39216691 | MsG0680032355.01.T01:CDS | 35.0% | |
| TATGAGACTGAGAACAATCT+TGG | + | Chr6:39216950-39216969 | None:intergenic | 35.0% | |
| TTTGCACCCTACCTATATAT+TGG | - | Chr6:39217008-39217027 | MsG0680032355.01.T01:intron | 35.0% | |
| ! | GTAGTTTTCCTATGTTCGAT+GGG | - | Chr6:39217110-39217129 | MsG0680032355.01.T01:CDS | 35.0% |
| ! | GTTGTATTTTGTACTGCTGA+AGG | - | Chr6:39217202-39217221 | MsG0680032355.01.T01:intron | 35.0% |
| ! | TAGTTTTCCTATGTTCGATG+GGG | - | Chr6:39217111-39217130 | MsG0680032355.01.T01:CDS | 35.0% |
| ! | TGTAGTTTTCCTATGTTCGA+TGG | - | Chr6:39217109-39217128 | MsG0680032355.01.T01:CDS | 35.0% |
| !! | AACACGTTTTTCAGCAAAAC+TGG | + | Chr6:39216773-39216792 | None:intergenic | 35.0% |
| !! | ACACGTTTTTCAGCAAAACT+GGG | + | Chr6:39216772-39216791 | None:intergenic | 35.0% |
| !! | CTACTTTAAAGCACAAACAG+AGG | + | Chr6:39216729-39216748 | None:intergenic | 35.0% |
| !! | GAAGGGTATATTAAGTTGAG+CGG | - | Chr6:39217305-39217324 | MsG0680032355.01.T01:intron | 35.0% |
| GAACGCCTATGTACTCAAAT+GGG | - | Chr6:39216870-39216889 | MsG0680032355.01.T01:CDS | 40.0% | |
| GCTACACGATTCTACGAATA+TGG | - | Chr6:39216671-39216690 | MsG0680032355.01.T01:CDS | 40.0% | |
| TTTCCAAATGTAGTCGCCAT+TGG | - | Chr6:39217054-39217073 | MsG0680032355.01.T01:CDS | 40.0% | |
| ATAACCGCAAAGCTGTTCGA+GGG | - | Chr6:39216969-39216988 | MsG0680032355.01.T01:intron | 45.0% | |
| CACGATTCTACGAATATGGG+TGG | - | Chr6:39216675-39216694 | MsG0680032355.01.T01:CDS | 45.0% | |
| GGAACGCCTATGTACTCAAA+TGG | - | Chr6:39216869-39216888 | MsG0680032355.01.T01:CDS | 45.0% | |
| TATAACCGCAAAGCTGTTCG+AGG | - | Chr6:39216968-39216987 | MsG0680032355.01.T01:intron | 45.0% | |
| TGCTGAAGGCTCTTTACTCT+GGG | - | Chr6:39217216-39217235 | MsG0680032355.01.T01:intron | 45.0% | |
| TTGCCAATGGCGACTACATT+TGG | + | Chr6:39217060-39217079 | None:intergenic | 45.0% | |
| TTTACCCTCGAACAGCTTTG+CGG | + | Chr6:39216976-39216995 | None:intergenic | 45.0% | |
| ! | TTTTCCTATGTTCGATGGGG+TGG | - | Chr6:39217114-39217133 | MsG0680032355.01.T01:CDS | 45.0% |
| AAAGCCGAGGAAGCCATCAT+GGG | - | Chr6:39217334-39217353 | MsG0680032355.01.T01:CDS | 50.0% | |
| CTGCTGAAGGCTCTTTACTC+TGG | - | Chr6:39217215-39217234 | MsG0680032355.01.T01:intron | 50.0% | |
| GTATCCCATGATGGCTTCCT+CGG | + | Chr6:39217341-39217360 | None:intergenic | 50.0% | |
| !! | ACTGTGGGAGTATCCCATGA+TGG | + | Chr6:39217350-39217369 | None:intergenic | 50.0% |
| ATGTTCGATGGGGTGGAAGC+CGG | - | Chr6:39217121-39217140 | MsG0680032355.01.T01:CDS | 55.0% | |
| GAAAGCCGAGGAAGCCATCA+TGG | - | Chr6:39217333-39217352 | MsG0680032355.01.T01:CDS | 55.0% | |
| GCTTCCACCCCATCGAACAT+AGG | + | Chr6:39217121-39217140 | None:intergenic | 55.0% | |
| TGAGCGGTTTGAGAAAGCCG+AGG | - | Chr6:39217321-39217340 | MsG0680032355.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 39216640 | 39217376 | 39216640 | ID=MsG0680032355.01;Name=MsG0680032355.01 |
| Chr6 | mRNA | 39216640 | 39217376 | 39216640 | ID=MsG0680032355.01.T01;Parent=MsG0680032355.01;Name=MsG0680032355.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|0.75|4|0|120 |
| Chr6 | exon | 39217318 | 39217376 | 39217318 | ID=MsG0680032355.01.T01:exon:21356;Parent=MsG0680032355.01.T01 |
| Chr6 | exon | 39217034 | 39217153 | 39217034 | ID=MsG0680032355.01.T01:exon:21355;Parent=MsG0680032355.01.T01 |
| Chr6 | exon | 39216845 | 39216949 | 39216845 | ID=MsG0680032355.01.T01:exon:21354;Parent=MsG0680032355.01.T01 |
| Chr6 | exon | 39216640 | 39216718 | 39216640 | ID=MsG0680032355.01.T01:exon:21353;Parent=MsG0680032355.01.T01 |
| Chr6 | CDS | 39217318 | 39217376 | 39217318 | ID=MsG0680032355.01.T01:cds;Parent=MsG0680032355.01.T01 |
| Chr6 | CDS | 39217034 | 39217153 | 39217034 | ID=MsG0680032355.01.T01:cds;Parent=MsG0680032355.01.T01 |
| Chr6 | CDS | 39216845 | 39216949 | 39216845 | ID=MsG0680032355.01.T01:cds;Parent=MsG0680032355.01.T01 |
| Chr6 | CDS | 39216640 | 39216718 | 39216640 | ID=MsG0680032355.01.T01:cds;Parent=MsG0680032355.01.T01 |
| Gene Sequence |
| Protein sequence |
