Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042966.01.T01 | XP_003627527.2 | 97.228 | 505 | 14 | 0 | 1 | 505 | 1 | 505 | 0 | 1034 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0880042966.01 | 0.847834 | 8.996603e-60 | 1.619358e-56 |
| MsG0080047944.01 | MsG0880042966.01 | 0.809130 | 2.203306e-50 | 1.283072e-47 |
| MsG0080047957.01 | MsG0880042966.01 | 0.825931 | 3.581128e-54 | 3.310484e-51 |
| MsG0080047959.01 | MsG0880042966.01 | 0.831713 | 1.428483e-55 | 1.563034e-52 |
| MsG0080048404.01 | MsG0880042966.01 | 0.812356 | 4.418262e-51 | 2.803136e-48 |
| MsG0080048462.01 | MsG0880042966.01 | 0.811610 | 6.422135e-51 | 3.993323e-48 |
| MsG0080048618.01 | MsG0880042966.01 | 0.806878 | 6.641417e-50 | 3.645739e-47 |
| MsG0080048683.01 | MsG0880042966.01 | 0.813965 | 1.959168e-51 | 1.297832e-48 |
| MsG0080048764.01 | MsG0880042966.01 | 0.839579 | 1.456152e-57 | 2.019564e-54 |
| MsG0080048785.01 | MsG0880042966.01 | 0.802726 | 4.892858e-49 | 2.414535e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880042966.01 | MsG0380014173.01 | PPI |
| MsG0880042966.01 | MsG0880045133.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042966.01.T01 | MTR_8g027150 | 97.228 | 505 | 14 | 0 | 1 | 505 | 1 | 505 | 0.0 | 1034 |
| MsG0880042966.01.T01 | MTR_3g109380 | 39.798 | 495 | 286 | 6 | 1 | 485 | 23 | 515 | 4.35e-136 | 404 |
| MsG0880042966.01.T01 | MTR_8g086040 | 40.159 | 503 | 283 | 7 | 1 | 490 | 24 | 521 | 1.10e-127 | 385 |
| MsG0880042966.01.T01 | MTR_1g083890 | 40.598 | 468 | 261 | 7 | 3 | 458 | 24 | 486 | 2.51e-124 | 372 |
| MsG0880042966.01.T01 | MTR_7g056073 | 37.626 | 497 | 273 | 4 | 31 | 491 | 27 | 522 | 5.36e-119 | 360 |
| MsG0880042966.01.T01 | MTR_2g087120 | 39.530 | 511 | 277 | 10 | 3 | 502 | 19 | 508 | 2.15e-118 | 358 |
| MsG0880042966.01.T01 | MTR_6g075280 | 37.292 | 480 | 280 | 5 | 7 | 479 | 36 | 501 | 1.24e-116 | 359 |
| MsG0880042966.01.T01 | MTR_6g075280 | 26.562 | 320 | 194 | 6 | 118 | 399 | 2 | 318 | 3.39e-30 | 125 |
| MsG0880042966.01.T01 | MTR_5g020650 | 36.789 | 492 | 300 | 4 | 1 | 483 | 24 | 513 | 4.23e-116 | 352 |
| MsG0880042966.01.T01 | MTR_1g012380 | 36.614 | 508 | 271 | 9 | 1 | 490 | 31 | 505 | 6.52e-115 | 352 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042966.01.T01 | AT5G08510 | 57.738 | 504 | 209 | 2 | 1 | 500 | 1 | 504 | 0.0 | 598 |
| MsG0880042966.01.T01 | AT2G20540 | 40.082 | 489 | 283 | 5 | 2 | 482 | 24 | 510 | 3.08e-131 | 392 |
| MsG0880042966.01.T01 | AT3G29230 | 36.494 | 559 | 282 | 6 | 1 | 487 | 32 | 589 | 1.97e-124 | 376 |
| MsG0880042966.01.T01 | AT5G56310 | 40.000 | 495 | 281 | 6 | 1 | 482 | 28 | 519 | 2.20e-124 | 374 |
| MsG0880042966.01.T01 | AT2G20540 | 43.980 | 407 | 226 | 2 | 77 | 483 | 77 | 481 | 1.89e-123 | 370 |
| MsG0880042966.01.T01 | AT2G20540 | 23.837 | 172 | 127 | 1 | 62 | 229 | 194 | 365 | 5.32e-12 | 68.6 |
| MsG0880042966.01.T01 | AT4G37380 | 39.920 | 501 | 285 | 6 | 1 | 490 | 42 | 537 | 6.92e-122 | 371 |
| MsG0880042966.01.T01 | AT5G06540 | 38.269 | 520 | 301 | 7 | 2 | 503 | 26 | 543 | 1.21e-121 | 370 |
| MsG0880042966.01.T01 | AT5G06540 | 21.552 | 348 | 211 | 7 | 85 | 401 | 18 | 334 | 3.43e-21 | 97.4 |
| MsG0880042966.01.T01 | AT5G44230 | 39.024 | 492 | 283 | 9 | 1 | 478 | 62 | 550 | 5.00e-121 | 369 |
Find 137 sgRNAs with CRISPR-Local
Find 174 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCAGATTTCATGAACT+GGG | 0.550222 | 8:-16800639 | None:intergenic |
| AAAGAGCGATGGAATTGTTT+TGG | 0.399352 | 8:+16800816 | MsG0880042966.01.T01:CDS |
| AAAGCTCAGAAAGGTGATGA+AGG | 0.534075 | 8:+16802023 | MsG0880042966.01.T01:CDS |
| AAATGAATATGAATGTCAGT+TGG | 0.533316 | 8:+16802194 | MsG0880042966.01.T01:CDS |
| AAATTTCGCTACTTTCTGAA+TGG | 0.413796 | 8:-16802128 | None:intergenic |
| AACAACTAGTCATAGGGAGT+AGG | 0.597653 | 8:+16802228 | MsG0880042966.01.T01:exon |
| AACCAAGTGAAACAAATTCA+TGG | 0.424076 | 8:+16800335 | MsG0880042966.01.T01:CDS |
| AACCATGAATTTGTTTCACT+TGG | 0.497562 | 8:-16800337 | None:intergenic |
| AACTCCGTCCCATTTCCCAG+CGG | 0.705483 | 8:-16801998 | None:intergenic |
| AAGCAGTATGAGAAGGCTTT+GGG | 0.338895 | 8:+16800899 | MsG0880042966.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACCAGATTTCATGAACT+GGG | - | Chr8:16800642-16800661 | None:intergenic | 30.0% | |
| AACCAAGTGAAACAAATTCA+TGG | + | Chr8:16800335-16800354 | MsG0880042966.01.T01:CDS | 30.0% | |
| ATTCCCAAACTAGAACATTA+TGG | + | Chr8:16801754-16801773 | MsG0880042966.01.T01:CDS | 30.0% | |
| CATTTGATCATAAAGCTGAA+TGG | - | Chr8:16801227-16801246 | None:intergenic | 30.0% | |
| CCCTAAAATAACTTCCAAAA+AGG | - | Chr8:16801307-16801326 | None:intergenic | 30.0% | |
| GATTTGAACAACTAGTCATA+GGG | + | Chr8:16802222-16802241 | MsG0880042966.01.T01:exon | 30.0% | |
| TAAAACCAGATTTCATGAAC+TGG | - | Chr8:16800643-16800662 | None:intergenic | 30.0% | |
| TGATTTGAACAACTAGTCAT+AGG | + | Chr8:16802221-16802240 | MsG0880042966.01.T01:exon | 30.0% | |
| AAGTGTATGCGAGGAAAAAT+GGG | + | Chr8:16801035-16801054 | MsG0880042966.01.T01:CDS | 35.0% | |
| ACAGTACTTGTGCATAGTTT+AGG | - | Chr8:16800427-16800446 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 16800259 | 16802360 | 16800259 | ID=MsG0880042966.01;Name=MsG0880042966.01 |
| Chr8 | mRNA | 16800259 | 16802360 | 16800259 | ID=MsG0880042966.01.T01;Parent=MsG0880042966.01;Name=MsG0880042966.01.T01;_AED=0.01;_eAED=0.01;_QI=73|1|1|1|1|1|2|124|506 |
| Chr8 | exon | 16800259 | 16801249 | 16800259 | ID=MsG0880042966.01.T01:exon:3197;Parent=MsG0880042966.01.T01 |
| Chr8 | exon | 16801634 | 16802360 | 16801634 | ID=MsG0880042966.01.T01:exon:3198;Parent=MsG0880042966.01.T01 |
| Chr8 | five_prime_UTR | 16800259 | 16800331 | 16800259 | ID=MsG0880042966.01.T01:five_prime_utr;Parent=MsG0880042966.01.T01 |
| Chr8 | CDS | 16800332 | 16801249 | 16800332 | ID=MsG0880042966.01.T01:cds;Parent=MsG0880042966.01.T01 |
| Chr8 | CDS | 16801634 | 16802236 | 16801634 | ID=MsG0880042966.01.T01:cds;Parent=MsG0880042966.01.T01 |
| Chr8 | three_prime_UTR | 16802237 | 16802360 | 16802237 | ID=MsG0880042966.01.T01:three_prime_utr;Parent=MsG0880042966.01.T01 |
| Gene Sequence |
>MsG0880042966.01.T01 ATGAACCAAGTGAAACAAATTCATGGTTACACACTAAGAAATAACATAGACAACACCAAAATCCTCATTGAAAAACTACTTCAAATCCCAAACCTAAACTATGCACAAGTACTGTTACACCATTCTCAAAAACCAACCACTTTCCTCTACAACAAACTCATTCAAGCATGTTCCTCAAAACATCAATGTTTCACCCTTTACTCCCAAATGTGCCTTCATGGTCACTCACCAAATCAATACACTTTCAACTTCCTTTTCACTACATGTACCTCACTTTCCTCACTTTCCCTTGGCCAAATGATCCATACCCAGTTCATGAAATCTGGTTTTAAACATGATGTATTTGCTTCAACTGCTTTGCTTGATTTGTACGCTAAATTGGGTTGTTTGAAATTTGCACGCAATGTGTTTGACGAAATGTCTGTGAGAGAGTTGGCTACGTGGAATGCGATGATGGCGGGGTGTACGAGGTTTGGTGATATGGAAAGAGCGATGGAATTGTTTTGGTTGATGCCTTCGAGGAATGTGGTGTCGTGGACTACTATGGTATCTGGTTATTCGCAAAATAAGCAGTATGAGAAGGCTTTGGGGTTGTTTTTGAGGATGGAAAGGGAGAAAGATGTGAGTCCGAATGAAGTTACGTTGGCAAGTGTTTTGCCGGCTTGTGCAAATCTTGGGGCACTTGAGATTGGGCAGAGGATTGAAGTGTATGCGAGGAAAAATGGGTTTTTTAAGAATCTGTTTGTATGCAATGCTGTGTTGGAGATGTATGCAAAATGTGGGAAGATTGATGTTGCTTGGAAGGTGTTTGATGAGATAGGTAGATTCAGAAATTTGTGCTCTTGGAATTCGATGATCATGGGTTTAGCTGTTCATGGACAATGTCACAAGGCCATTCAGCTTTATGATCAAATGTTGAGAGAAGGAACTCTGCCTGATGACGTGACATTCGTGGGGCTTCTCTTGGCATGCACACATGGAGGCATGGTTGAAAAAGGCAAGCATATATTCCAGTCAATGACAAGAGACTTCAGTATTATTCCCAAACTAGAACATTATGGCTGCATGGTTGATCTACTTGGTCGTGCTGGCCGATTAACAGAAGCTTACGAGGTCATAAAAAGAATGCCAATGAAGCCAGACTCGGTAATATGGGGTACTTTGTTGGGGGCTTGCAGTTTCCATGGTAATGTTGAATTGGCTGAGGTAGCAGCTGAGTCTTTATTTGTACTTGAGCCTTGGAATCCAGGAAATTACGTCATTCTTTCGAACATTTATGCATCCGCTGGGAAATGGGACGGAGTTGCAAAGCTCAGAAAGGTGATGAAGGGAAGCAAAATTACAAAGACAGCTGGACATAGCTTCATTGAAGAGGGAGGTCAACTGCATAAGTTCATTGTGGAGGATAGATCCCATTCAGAAAGTAGCGAAATTTTTGCTTTGCTTAATGGAGTTTATGAAATGATAAAGTTTAATAAAAATGAATATGAATGTCAGTTGGATTTTGATTTGAACAACTAG |
| Protein sequence |
>MsG0880042966.01.T01 MNQVKQIHGYTLRNNIDNTKILIEKLLQIPNLNYAQVLLHHSQKPTTFLYNKLIQACSSKHQCFTLYSQMCLHGHSPNQYTFNFLFTTCTSLSSLSLGQMIHTQFMKSGFKHDVFASTALLDLYAKLGCLKFARNVFDEMSVRELATWNAMMAGCTRFGDMERAMELFWLMPSRNVVSWTTMVSGYSQNKQYEKALGLFLRMEREKDVSPNEVTLASVLPACANLGALEIGQRIEVYARKNGFFKNLFVCNAVLEMYAKCGKIDVAWKVFDEIGRFRNLCSWNSMIMGLAVHGQCHKAIQLYDQMLREGTLPDDVTFVGLLLACTHGGMVEKGKHIFQSMTRDFSIIPKLEHYGCMVDLLGRAGRLTEAYEVIKRMPMKPDSVIWGTLLGACSFHGNVELAEVAAESLFVLEPWNPGNYVILSNIYASAGKWDGVAKLRKVMKGSKITKTAGHSFIEEGGQLHKFIVEDRSHSESSEIFALLNGVYEMIKFNKNEYECQLDFDLNN* |
