AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021276.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021276.01.T01 MTR_2g028130 88.889 63 6 1 17 79 221 282 1.86e-31 113
MsG0480021276.01.T01 MTR_1g068835 74.194 62 14 2 18 79 199 258 2.40e-22 88.6
MsG0480021276.01.T01 MTR_0667s0010 72.059 68 16 3 17 82 210 276 3.74e-22 88.2
MsG0480021276.01.T01 MTR_2g028140 65.672 67 20 2 15 79 223 288 8.76e-22 87.4
MsG0480021276.01.T01 MTR_2g028160 67.188 64 18 2 18 79 222 284 9.02e-22 87.4
MsG0480021276.01.T01 MTR_1g068875 70.769 65 17 2 18 82 198 260 1.51e-21 86.3
MsG0480021276.01.T01 MTR_8g099460 69.355 62 14 2 18 79 212 268 2.09e-21 86.3
MsG0480021276.01.T01 MTR_2g426070 72.581 62 13 2 18 79 224 281 3.28e-21 85.5
MsG0480021276.01.T01 MTR_2g028110 63.077 65 20 2 15 79 204 264 1.30e-20 84.0
MsG0480021276.01.T01 MTR_1g068860 66.129 62 19 2 18 79 201 260 2.00e-20 83.2
MsG0480021276.01.T01 MTR_2g025420 68.182 66 16 3 18 79 225 289 2.18e-20 83.6
MsG0480021276.01.T01 MTR_4g070550 64.062 64 20 2 18 79 220 282 2.37e-20 83.2
MsG0480021276.01.T01 MTR_2g426090 70.312 64 15 2 18 81 220 279 3.32e-20 82.8
MsG0480021276.01.T01 MTR_2g031680 59.494 79 23 4 6 82 191 262 2.14e-19 80.5
MsG0480021276.01.T01 MTR_2g024100 65.152 66 18 3 18 79 213 277 2.65e-18 77.4
MsG0480021276.01.T01 MTR_2g025960 60.000 65 21 2 18 82 126 185 6.05e-18 75.9
MsG0480021276.01.T01 MTR_2g037520 66.154 65 18 2 18 82 2 62 9.59e-18 73.2
MsG0480021276.01.T01 MTR_2g021050 63.077 65 20 3 18 79 189 252 3.62e-17 74.3
MsG0480021276.01.T01 MTR_2g425980 66.667 66 19 3 18 81 217 281 1.28e-16 72.8
MsG0480021276.01.T01 MTR_2g426060 60.000 65 22 3 18 79 218 281 2.74e-16 72.0
MsG0480021276.01.T01 MTR_2g031640 61.429 70 21 4 18 82 198 266 7.29e-16 70.9
MsG0480021276.01.T01 MTR_2g026010 59.722 72 19 4 18 81 197 266 3.16e-15 68.9
MsG0480021276.01.T01 MTR_2g031720 60.606 66 21 4 18 79 200 264 3.80e-15 68.6
MsG0480021276.01.T01 MTR_2g426020 62.295 61 16 3 18 77 218 272 4.00e-15 68.6
MsG0480021276.01.T01 MTR_1g068905 64.912 57 18 2 18 74 182 236 4.15e-15 68.2
MsG0480021276.01.T01 MTR_2g024110 52.113 71 28 3 7 77 189 253 7.36e-14 65.1
MsG0480021276.01.T01 MTR_3g083010 53.125 64 25 2 18 79 227 287 6.25e-13 62.4
MsG0480021276.01.T01 MTR_2g031700 66.000 50 10 2 6 55 191 233 1.23e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAACATTTATACGATTT+GGG 0.223285 4:-56195782 MsG0480021276.01.T01:CDS
TCTTCAACATTTATACGATT+TGG 0.233050 4:-56195783 MsG0480021276.01.T01:CDS
TAACCCACCAAGTTTCTTCT+TGG 0.278198 4:+56195725 None:intergenic
CATTGAAAGTCTCAAGTGTT+AGG 0.365530 4:+56195676 None:intergenic
GAAATAGGTGAAGAAGTTAA+TGG 0.373645 4:-56195636 MsG0480021276.01.T01:CDS
GTTCCTCTTCCTGATGAAAT+AGG 0.374911 4:-56195651 MsG0480021276.01.T01:CDS
TTGTTGAGAATTCTATTCAC+TGG 0.447743 4:-56195755 MsG0480021276.01.T01:CDS
ACTTCTTCACCTATTTCATC+AGG 0.451165 4:+56195642 None:intergenic
TGAAGATCGTACGGCATAGC+AGG 0.487119 4:+56195800 None:intergenic
TCACCTATTTCATCAGGAAG+AGG 0.521517 4:+56195648 None:intergenic
TTCAACATTTATACGATTTG+GGG 0.558130 4:-56195781 MsG0480021276.01.T01:CDS
ATTATGACCAAGAAGAAACT+TGG 0.569795 4:-56195732 MsG0480021276.01.T01:CDS
TATAAATGTTGAAGATCGTA+CGG 0.573781 4:+56195791 None:intergenic
ATGACCAAGAAGAAACTTGG+TGG 0.584801 4:-56195729 MsG0480021276.01.T01:CDS
AACACTTGAGACTTTCAATG+AGG 0.585762 4:-56195673 MsG0480021276.01.T01:CDS
TTAAACGCGACAATTAAAGA+AGG 0.597351 4:+56195699 None:intergenic
TGACCAAGAAGAAACTTGGT+GGG 0.626228 4:-56195728 MsG0480021276.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTTCAACATTTATACGATTT+GGG - Chr4:56195618-56195637 MsG0480021276.01.T01:CDS 25.0%
! TATAAATGTTGAAGATCGTA+CGG + Chr4:56195612-56195631 None:intergenic 25.0%
! TCTTCAACATTTATACGATT+TGG - Chr4:56195617-56195636 MsG0480021276.01.T01:CDS 25.0%
! TTCAACATTTATACGATTTG+GGG - Chr4:56195619-56195638 MsG0480021276.01.T01:CDS 25.0%
ATTATGACCAAGAAGAAACT+TGG - Chr4:56195668-56195687 MsG0480021276.01.T01:CDS 30.0%
GAAATAGGTGAAGAAGTTAA+TGG - Chr4:56195764-56195783 MsG0480021276.01.T01:CDS 30.0%
TTAAACGCGACAATTAAAGA+AGG + Chr4:56195704-56195723 None:intergenic 30.0%
TTGTTGAGAATTCTATTCAC+TGG - Chr4:56195645-56195664 MsG0480021276.01.T01:CDS 30.0%
AACACTTGAGACTTTCAATG+AGG - Chr4:56195727-56195746 MsG0480021276.01.T01:CDS 35.0%
ACTTCTTCACCTATTTCATC+AGG + Chr4:56195761-56195780 None:intergenic 35.0%
CATTGAAAGTCTCAAGTGTT+AGG + Chr4:56195727-56195746 None:intergenic 35.0%
ATGACCAAGAAGAAACTTGG+TGG - Chr4:56195671-56195690 MsG0480021276.01.T01:CDS 40.0%
GTTCCTCTTCCTGATGAAAT+AGG - Chr4:56195749-56195768 MsG0480021276.01.T01:CDS 40.0%
TAACCCACCAAGTTTCTTCT+TGG + Chr4:56195678-56195697 None:intergenic 40.0%
TCACCTATTTCATCAGGAAG+AGG + Chr4:56195755-56195774 None:intergenic 40.0%
TGACCAAGAAGAAACTTGGT+GGG - Chr4:56195672-56195691 MsG0480021276.01.T01:CDS 40.0%
!!! AGTTCGTGTTGCTGTTTTAG+AGG - Chr4:56195799-56195818 MsG0480021276.01.T01:CDS 40.0%
!!! GTTCGTGTTGCTGTTTTAGA+GGG - Chr4:56195800-56195819 MsG0480021276.01.T01:CDS 40.0%
TGAAGATCGTACGGCATAGC+AGG + Chr4:56195603-56195622 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 56195587 56195835 56195587 ID=MsG0480021276.01;Name=MsG0480021276.01
Chr4 mRNA 56195587 56195835 56195587 ID=MsG0480021276.01.T01;Parent=MsG0480021276.01;Name=MsG0480021276.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|82
Chr4 exon 56195587 56195835 56195587 ID=MsG0480021276.01.T01:exon:6871;Parent=MsG0480021276.01.T01
Chr4 CDS 56195587 56195835 56195587 ID=MsG0480021276.01.T01:cds;Parent=MsG0480021276.01.T01
Gene Sequence

>MsG0480021276.01.T01

ATGGAAGATAATTCCTGCTATGCCGTACGATCTTCAACATTTATACGATTTGGGGTTTTTGTTGAGAATTCTATTCACTGGATTATGACCAAGAAGAAACTTGGTGGGTTACATCCTTCTTTAATTGTCGCGTTTAACCTAACACTTGAGACTTTCAATGAGGTTCCTCTTCCTGATGAAATAGGTGAAGAAGTTAATGGTAAGAGTTTTGAAGTTCGTGTTGCTGTTTTAGAGGGTGTCTTTGTATGA

Protein sequence

>MsG0480021276.01.T01

MEDNSCYAVRSSTFIRFGVFVENSIHWIMTKKKLGGLHPSLIVAFNLTLETFNEVPLPDEIGEEVNGKSFEVRVAVLEGVFV*