AlfalfaGEDB | MsG0880046004.01

M. sativa cultivar ZhongmuNo.1 / MsG0880046004.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046004.01.T01	XP_024636505.1	91.852	135	10	1	1	134	16	150	5.26E-83	250

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046004.01.T01	G7JF71	91.852	135	10	1	1	134	16	150	2.51e-83	250

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048856.01	MsG0880046004.01	0.803930	2.755726e-49	1.402538e-46
MsG0180000139.01	MsG0880046004.01	0.812730	3.659510e-51	2.344805e-48
MsG0180000470.01	MsG0880046004.01	0.806811	6.862700e-50	3.760781e-47
MsG0880045164.01	MsG0880046004.01	0.857863	1.221574e-62	3.058095e-59
MsG0880046004.01	MsG0880047473.01	0.813880	2.046051e-51	1.352287e-48
MsG0180003403.01	MsG0880046004.01	0.803604	3.221069e-49	1.625612e-46
MsG0180003455.01	MsG0880046004.01	0.817833	2.685446e-52	1.977061e-49
MsG0180004481.01	MsG0880046004.01	0.805833	1.102852e-49	5.894330e-47
MsG0180004482.01	MsG0880046004.01	0.808329	3.267185e-50	1.862873e-47
MsG0180006024.01	MsG0880046004.01	0.824017	1.013733e-53	8.871733e-51
MsG0280010788.01	MsG0880046004.01	0.860282	2.303118e-63	6.261665e-60
MsG0280011329.01	MsG0880046004.01	0.827889	1.219152e-54	1.192531e-51
MsG0380014221.01	MsG0880046004.01	0.802558	5.298513e-49	2.603329e-46
MsG0380014537.01	MsG0880046004.01	0.820394	7.019516e-53	5.547830e-50
MsG0680030882.01	MsG0880046004.01	0.808889	2.481006e-50	1.435377e-47
MsG0680031263.01	MsG0880046004.01	0.819003	1.459064e-52	1.109452e-49
MsG0680031264.01	MsG0880046004.01	0.852000	6.151587e-61	1.267382e-57
MsG0680031617.01	MsG0880046004.01	0.803965	2.709279e-49	1.380084e-46
MsG0680031621.01	MsG0880046004.01	0.821768	3.387241e-53	2.781507e-50
MsG0680032771.01	MsG0880046004.01	0.801064	1.074003e-48	5.081123e-46
MsG0680033412.01	MsG0880046004.01	0.800936	1.140426e-48	5.377856e-46
MsG0480021056.01	MsG0880046004.01	0.858985	5.653459e-63	1.470001e-59
MsG0480022054.01	MsG0880046004.01	0.837821	4.144611e-57	5.448141e-54
MsG0480023463.01	MsG0880046004.01	0.803583	3.252692e-49	1.640774e-46
MsG0580025135.01	MsG0880046004.01	0.800245	1.577800e-48	7.310694e-46
MsG0580029236.01	MsG0880046004.01	0.800999	1.107388e-48	5.230628e-46
MsG0680030447.01	MsG0880046004.01	0.840668	7.571050e-58	1.086135e-54
MsG0280006910.01	MsG0880046004.01	0.803410	3.533101e-49	1.774146e-46
MsG0280007955.01	MsG0880046004.01	0.814525	1.473681e-51	9.912027e-49
MsG0280008615.01	MsG0880046004.01	0.802591	5.217730e-49	2.565900e-46
MsG0280009421.01	MsG0880046004.01	0.801703	7.943860e-49	3.819078e-46
MsG0780037054.01	MsG0880046004.01	0.813085	3.059099e-51	1.978542e-48
MsG0780039940.01	MsG0880046004.01	0.804714	1.892380e-49	9.826949e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046004.01.T01	MTR_4g097260	91.852	135	10	1	1	134	16	150	6.36e-87	250
MsG0880046004.01.T01	MTR_4g097250	57.273	110	33	6	1	106	17	116	2.66e-29	103
MsG0880046004.01.T01	MTR_3g103290	32.432	148	74	6	1	131	17	155	9.28e-13	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTTACCAGGAACCTTAA+TGG	0.240275	8:+67456094	None:intergenic
GGTACATGAAGGCACCATTA+AGG	0.380437	8:-67456108	None:intergenic
CGGAGTCGCAGTGTTGGAAT+TGG	0.390004	8:-67455839	MsG0880046004.01.T01:CDS
GATGAAAATCGTGTTGAGTT+AGG	0.392028	8:-67455884	MsG0880046004.01.T01:CDS
GTAGGACATCCCATGGTTGC+AGG	0.417097	8:+67456013	None:intergenic
CACAAGTGATGATGATTTCA+AGG	0.436445	8:-67455939	MsG0880046004.01.T01:CDS
GAAGGCACCATTAAGGTTCC+TGG	0.439699	8:-67456101	MsG0880046004.01.T01:CDS
CGCAACTTTACTCCTTTCCT+AGG	0.451673	8:-67455991	MsG0880046004.01.T01:CDS
ACCATTAAGGTTCCTGGTAA+AGG	0.453532	8:-67456095	MsG0880046004.01.T01:CDS
TTCTGCTTCTAGAATAAAGA+AGG	0.463761	8:+67455756	None:intergenic
CTATGTAGATCCTGCAACCA+TGG	0.486263	8:-67456023	MsG0880046004.01.T01:CDS
AAGGTTCCTGGTAAAGGTAA+AGG	0.501075	8:-67456089	MsG0880046004.01.T01:CDS
GAGTTAACACTGAAGCTCCT+AGG	0.519744	8:+67455974	None:intergenic
TCGTAAGGGCTGCGTCACTT+AGG	0.537263	8:-67455913	MsG0880046004.01.T01:CDS
GTGCCTCGGAGTCGCAGTGT+TGG	0.539129	8:-67455845	MsG0880046004.01.T01:CDS
GTAAAGGACATGTACGTCAA+TGG	0.539882	8:-67456073	MsG0880046004.01.T01:CDS
ACATGTCCTTTACCTTTACC+AGG	0.540520	8:+67456083	None:intergenic
ATGATTTCAAGGAACTCGTA+AGG	0.547916	8:-67455928	MsG0880046004.01.T01:CDS
TGAAGATGATGACAATATCA+AGG	0.548852	8:-67455786	MsG0880046004.01.T01:CDS
TCTGCTTCTAGAATAAAGAA+GGG	0.562308	8:+67455757	None:intergenic
CATGGTTGCAGGATCTACAT+AGG	0.567986	8:+67456024	None:intergenic
CATATTTGCTCTACTGCGAA+TGG	0.570269	8:+67455727	None:intergenic
TATGTAGATCCTGCAACCAT+GGG	0.570418	8:-67456022	MsG0880046004.01.T01:CDS
CTTGAAATCATCATCACTTG+TGG	0.583473	8:+67455940	None:intergenic
GTCGCAGTGTTGGAATTGGA+AGG	0.585409	8:-67455835	MsG0880046004.01.T01:CDS
AACACTGAAGCTCCTAGGAA+AGG	0.606093	8:+67455979	None:intergenic
GGAAAGGAGTAAAGTTGCGT+AGG	0.616682	8:+67455995	None:intergenic
CGAAAGCGATGAAAGTGCCT+CGG	0.617025	8:-67455859	MsG0880046004.01.T01:CDS
TGATTTCAAGGAACTCGTAA+GGG	0.632665	8:-67455927	MsG0880046004.01.T01:CDS
CTGCTTCTAGAATAAAGAAG+GGG	0.634070	8:+67455758	None:intergenic
AAGTTGCGTAGGACATCCCA+TGG	0.638342	8:+67456006	None:intergenic
ATTCCAACACTGCGACTCCG+AGG	0.689214	8:+67455842	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TGAAGATGATGACAATATCA+AGG	-	Chr8:67456038-67456057	MsG0880046004.01.T01:CDS	30.0%
!!	TCTGCTTCTAGAATAAAGAA+GGG	+	Chr8:67456070-67456089	None:intergenic	30.0%
!!	TTCTGCTTCTAGAATAAAGA+AGG	+	Chr8:67456071-67456090	None:intergenic	30.0%
	AGGAAAAATCACGAGAACAT+TGG	+	Chr8:67455783-67455802	None:intergenic	35.0%
	ATGATTTCAAGGAACTCGTA+AGG	-	Chr8:67455896-67455915	MsG0880046004.01.T01:CDS	35.0%
	CTTGAAATCATCATCACTTG+TGG	+	Chr8:67455887-67455906	None:intergenic	35.0%
	GATGAAAATCGTGTTGAGTT+AGG	-	Chr8:67455940-67455959	MsG0880046004.01.T01:CDS	35.0%
	TGATTTCAAGGAACTCGTAA+GGG	-	Chr8:67455897-67455916	MsG0880046004.01.T01:CDS	35.0%
!	CACAAGTGATGATGATTTCA+AGG	-	Chr8:67455885-67455904	MsG0880046004.01.T01:CDS	35.0%
!!	CTGCTTCTAGAATAAAGAAG+GGG	+	Chr8:67456069-67456088	None:intergenic	35.0%
	AAGGTTCCTGGTAAAGGTAA+AGG	-	Chr8:67455735-67455754	MsG0880046004.01.T01:CDS	40.0%
	ACATGTCCTTTACCTTTACC+AGG	+	Chr8:67455744-67455763	None:intergenic	40.0%
	ACCATTAAGGTTCCTGGTAA+AGG	-	Chr8:67455729-67455748	MsG0880046004.01.T01:CDS	40.0%
	ACCTTTACCAGGAACCTTAA+TGG	+	Chr8:67455733-67455752	None:intergenic	40.0%
	CATATTTGCTCTACTGCGAA+TGG	+	Chr8:67456100-67456119	None:intergenic	40.0%
	GTAAAGGACATGTACGTCAA+TGG	-	Chr8:67455751-67455770	MsG0880046004.01.T01:CDS	40.0%
	TATGTAGATCCTGCAACCAT+GGG	-	Chr8:67455802-67455821	MsG0880046004.01.T01:CDS	40.0%
	AACACTGAAGCTCCTAGGAA+AGG	+	Chr8:67455848-67455867	None:intergenic	45.0%
	CATGGTTGCAGGATCTACAT+AGG	+	Chr8:67455803-67455822	None:intergenic	45.0%
	CGCAACTTTACTCCTTTCCT+AGG	-	Chr8:67455833-67455852	MsG0880046004.01.T01:CDS	45.0%
	CTATGTAGATCCTGCAACCA+TGG	-	Chr8:67455801-67455820	MsG0880046004.01.T01:CDS	45.0%
	GAGTTAACACTGAAGCTCCT+AGG	+	Chr8:67455853-67455872	None:intergenic	45.0%
	GGAAAGGAGTAAAGTTGCGT+AGG	+	Chr8:67455832-67455851	None:intergenic	45.0%
	AAGTTGCGTAGGACATCCCA+TGG	+	Chr8:67455821-67455840	None:intergenic	50.0%
!	GTCGCAGTGTTGGAATTGGA+AGG	-	Chr8:67455989-67456008	MsG0880046004.01.T01:CDS	50.0%
!!	CGAAAGCGATGAAAGTGCCT+CGG	-	Chr8:67455965-67455984	MsG0880046004.01.T01:CDS	50.0%
!!	GAAGGCACCATTAAGGTTCC+TGG	-	Chr8:67455723-67455742	MsG0880046004.01.T01:CDS	50.0%
	ATTCCAACACTGCGACTCCG+AGG	+	Chr8:67455985-67456004	None:intergenic	55.0%
	GTAGGACATCCCATGGTTGC+AGG	+	Chr8:67455814-67455833	None:intergenic	55.0%
	TCGTAAGGGCTGCGTCACTT+AGG	-	Chr8:67455911-67455930	MsG0880046004.01.T01:CDS	55.0%
!	CGGAGTCGCAGTGTTGGAAT+TGG	-	Chr8:67455985-67456004	MsG0880046004.01.T01:CDS	55.0%
	GTGCCTCGGAGTCGCAGTGT+TGG	-	Chr8:67455979-67455998	MsG0880046004.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	67455721	67456125	67455721	ID=MsG0880046004.01;Name=MsG0880046004.01
Chr8	mRNA	67455721	67456125	67455721	ID=MsG0880046004.01.T01;Parent=MsG0880046004.01;Name=MsG0880046004.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|134
Chr8	exon	67455721	67456125	67455721	ID=MsG0880046004.01.T01:exon:29713;Parent=MsG0880046004.01.T01
Chr8	CDS	67455721	67456125	67455721	ID=MsG0880046004.01.T01:cds;Parent=MsG0880046004.01.T01

Gene Sequence

>MsG0880046004.01.T01

ATGAAGGCACCATTAAGGTTCCTGGTAAAGGTAAAGGACATGTACGTCAATGGCATGATCCAATGTTCTCGTGATTTTTCCTATGTAGATCCTGCAACCATGGGATGTCCTACGCAACTTTACTCCTTTCCTAGGAGCTTCAGTGTTAACTCTACAACATCGACCACAAGTGATGATGATTTCAAGGAACTCGTAAGGGCTGCGTCACTTAGGATCAGAGATGAAAATCGTGTTGAGTTAGGATCGAAAGCGATGAAAGTGCCTCGGAGTCGCAGTGTTGGAATTGGAAGGATTGAAGAAGACGAGATATGTGAGTTTGAAGATGATGACAATATCAAGGTCAAGCCCCTTCTTTATTCTAGAAGCAGAAGTTGTGCCATTCGCAGTAGAGCAAATATGTTTTAA

Protein sequence

>MsG0880046004.01.T01

MKAPLRFLVKVKDMYVNGMIQCSRDFSYVDPATMGCPTQLYSFPRSFSVNSTTSTTSDDDFKELVRAASLRIRDENRVELGSKAMKVPRSRSVGIGRIEEDEICEFEDDDNIKVKPLLYSRSRSCAIRSRANMF*