AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031617.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031617.01.T01 MTR_6g027550 96.450 169 6 0 1 169 149 317 3.37e-120 342
MsG0680031617.01.T01 MTR_7g086720 75.148 169 39 1 1 169 234 399 1.69e-92 275
MsG0680031617.01.T01 MTR_6g011080 51.190 168 79 1 1 168 268 432 2.59e-59 190
MsG0680031617.01.T01 MTR_0072s0020 52.663 169 73 4 1 168 270 432 3.76e-57 185
MsG0680031617.01.T01 MTR_4g022240 51.765 170 77 2 1 169 254 419 5.04e-57 184
MsG0680031617.01.T01 MTR_0662s0030 62.185 119 45 0 1 119 157 275 1.59e-51 166
MsG0680031617.01.T01 MTR_4g114140 62.185 119 45 0 1 119 256 374 1.03e-50 167
MsG0680031617.01.T01 MTR_2g085300 44.970 169 85 2 1 169 264 424 8.92e-50 166
MsG0680031617.01.T01 MTR_1g108280 43.023 172 88 3 2 169 232 397 1.70e-41 143
MsG0680031617.01.T01 MTR_4g008010 37.870 169 102 2 1 169 242 407 4.02e-33 121
MsG0680031617.01.T01 MTR_1g046740 38.690 168 97 2 1 168 277 438 4.51e-33 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031617.01.T01 AT5G15050 53.254 169 76 1 1 169 269 434 5.39e-64 202
MsG0680031617.01.T01 AT5G39990 51.786 168 78 1 2 169 283 447 1.44e-62 199
MsG0680031617.01.T01 AT1G03520 55.621 169 68 4 1 168 195 357 6.58e-61 192
MsG0680031617.01.T01 AT1G03520 55.621 169 68 4 1 168 284 446 3.21e-60 193
MsG0680031617.01.T01 AT3G03690 76.147 109 26 0 1 109 234 342 8.41e-58 185
MsG0680031617.01.T01 AT4G03340 53.846 169 71 4 1 168 262 424 9.09e-58 186
MsG0680031617.01.T01 AT4G27480 49.112 169 78 2 1 169 261 421 1.01e-57 186
MsG0680031617.01.T01 AT4G27480 49.112 169 78 2 1 169 261 421 1.01e-57 186
MsG0680031617.01.T01 AT4G27480 49.112 169 78 2 1 169 261 421 1.01e-57 186
MsG0680031617.01.T01 AT4G03340 53.846 169 71 4 1 168 285 447 1.46e-57 186
MsG0680031617.01.T01 AT3G15350 49.412 170 76 3 2 169 263 424 7.47e-56 181
MsG0680031617.01.T01 AT3G15350 49.412 170 76 3 2 169 263 424 7.47e-56 181
MsG0680031617.01.T01 AT1G53100 50.595 168 74 3 2 168 263 422 1.14e-54 178
MsG0680031617.01.T01 AT1G53100 51.899 158 67 3 2 158 263 412 7.43e-52 171
MsG0680031617.01.T01 AT1G71070 44.379 169 91 1 1 169 230 395 3.39e-48 160
MsG0680031617.01.T01 AT2G37585 45.238 168 70 3 2 169 239 384 1.18e-46 156
MsG0680031617.01.T01 AT3G24040 37.500 168 102 2 1 168 250 414 7.95e-32 118

Find 26 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCAAAGATTATAGGAAAA+TGG 0.261335 6:-23393329 MsG0680031617.01.T01:CDS
TCTTTGAGTGTTAGAGATCT+TGG 0.314388 6:+23393343 None:intergenic
CTCTAACACTCAAAGATTAT+AGG 0.319984 6:-23393336 MsG0680031617.01.T01:CDS
AGAGATCTTGGATGTTGTTT+TGG 0.322287 6:+23393355 None:intergenic
TTCTTAAACTTTCTAGCAAA+TGG 0.368091 6:+23393292 None:intergenic
ACTGTTTGGAAATACCCTTC+TGG 0.369138 6:+23393451 None:intergenic
GGTTTGAAAATTGAGAATTA+TGG 0.380635 6:-23393151 MsG0680031617.01.T01:CDS
TAGAATTGCATATAACTGTT+TGG 0.386470 6:+23393437 None:intergenic
TTTCTGAGTATTGTATTCTT+GGG 0.387883 6:-23393528 MsG0680031617.01.T01:CDS
ATGATCTACACTACATTACT+TGG 0.419908 6:-23393387 MsG0680031617.01.T01:CDS
CTGAGTATTGTATTCTTGGG+TGG 0.442957 6:-23393525 MsG0680031617.01.T01:CDS
TGGTGTTCTTAAGCCAAGTC+TGG 0.446844 6:-23393131 MsG0680031617.01.T01:CDS
ACACAAAATTTGTGTAGTAT+AGG 0.472913 6:+23393479 None:intergenic
TGAGTATTGTATTCTTGGGT+GGG 0.475364 6:-23393524 MsG0680031617.01.T01:CDS
CTATGCTTTCCACCTTGTGA+TGG 0.509851 6:+23393184 None:intergenic
CAAAATTTGTGTAGTATAGG+AGG 0.535652 6:+23393482 None:intergenic
TAGTATAGGAGGAGGATCCT+TGG 0.550571 6:+23393493 None:intergenic
TAAACTTTCTAGCAAATGGA+CGG 0.567234 6:+23393296 None:intergenic
TGATCTACACTACATTACTT+GGG 0.594137 6:-23393386 MsG0680031617.01.T01:CDS
AATTTGTGTAGTATAGGAGG+AGG 0.604508 6:+23393485 None:intergenic
GTGTAGATCATTGTTAGCTG+TGG 0.612568 6:+23393399 None:intergenic
ATTTACGCAAACAACAATGT+CGG 0.619006 6:-23393060 MsG0680031617.01.T01:CDS
TTGGGTGGGAAAATTTACCA+AGG 0.657816 6:-23393510 MsG0680031617.01.T01:CDS
GGAAAGCATAGAGCATGTCA+TGG 0.659761 6:-23393172 MsG0680031617.01.T01:CDS
TGGTGTTCATCACCATCACA+AGG 0.691054 6:-23393196 MsG0680031617.01.T01:CDS
TGTTCATCACCATCACAAGG+TGG 0.742385 6:-23393193 MsG0680031617.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATATTTTTCATCTTTTTTG+AGG + Chr6:23393496-23393515 None:intergenic 20.0%
! ACACAAAATTTGTGTAGTAT+AGG + Chr6:23393125-23393144 None:intergenic 25.0%
! ACTCAAAGATTATAGGAAAA+TGG - Chr6:23393272-23393291 MsG0680031617.01.T01:CDS 25.0%
! GGTTTGAAAATTGAGAATTA+TGG - Chr6:23393450-23393469 MsG0680031617.01.T01:CDS 25.0%
! TAGAATTGCATATAACTGTT+TGG + Chr6:23393167-23393186 None:intergenic 25.0%
!! TTCTTAAACTTTCTAGCAAA+TGG + Chr6:23393312-23393331 None:intergenic 25.0%
!! TTTCTGAGTATTGTATTCTT+GGG - Chr6:23393073-23393092 MsG0680031617.01.T01:CDS 25.0%
!!! TTTTCTGAGTATTGTATTCT+TGG - Chr6:23393072-23393091 MsG0680031617.01.T01:CDS 25.0%
ATGATCTACACTACATTACT+TGG - Chr6:23393214-23393233 MsG0680031617.01.T01:CDS 30.0%
ATTTACGCAAACAACAATGT+CGG - Chr6:23393541-23393560 MsG0680031617.01.T01:CDS 30.0%
CAAAATTTGTGTAGTATAGG+AGG + Chr6:23393122-23393141 None:intergenic 30.0%
CTCTAACACTCAAAGATTAT+AGG - Chr6:23393265-23393284 MsG0680031617.01.T01:CDS 30.0%
TGATCTACACTACATTACTT+GGG - Chr6:23393215-23393234 MsG0680031617.01.T01:CDS 30.0%
! AAATTGATAGAGAGCTTTTG+AGG - Chr6:23393352-23393371 MsG0680031617.01.T01:CDS 30.0%
! AATCGTGGACAATTTTCTTT+TGG - Chr6:23393378-23393397 MsG0680031617.01.T01:CDS 30.0%
! TAAACTTTCTAGCAAATGGA+CGG + Chr6:23393308-23393327 None:intergenic 30.0%
AATTTGTGTAGTATAGGAGG+AGG + Chr6:23393119-23393138 None:intergenic 35.0%
TCTTTGAGTGTTAGAGATCT+TGG + Chr6:23393261-23393280 None:intergenic 35.0%
! AATTTTGTGTCATCTCCAGA+AGG - Chr6:23393135-23393154 MsG0680031617.01.T01:CDS 35.0%
! ATTTTGTGTCATCTCCAGAA+GGG - Chr6:23393136-23393155 MsG0680031617.01.T01:CDS 35.0%
! TGAGTATTGTATTCTTGGGT+GGG - Chr6:23393077-23393096 MsG0680031617.01.T01:CDS 35.0%
!! AGAGATCTTGGATGTTGTTT+TGG + Chr6:23393249-23393268 None:intergenic 35.0%
!! GACAATTTTCTTTTGGAGGA+TGG - Chr6:23393385-23393404 MsG0680031617.01.T01:CDS 35.0%
ACTGTTTGGAAATACCCTTC+TGG + Chr6:23393153-23393172 None:intergenic 40.0%
GTGTAGATCATTGTTAGCTG+TGG + Chr6:23393205-23393224 None:intergenic 40.0%
TTGGGTGGGAAAATTTACCA+AGG - Chr6:23393091-23393110 MsG0680031617.01.T01:CDS 40.0%
! CGTGGACAATTTTCTTTTGG+AGG - Chr6:23393381-23393400 MsG0680031617.01.T01:CDS 40.0%
! CTGAGTATTGTATTCTTGGG+TGG - Chr6:23393076-23393095 MsG0680031617.01.T01:CDS 40.0%
!!! ATCTTTTTTGAGGCCAGACT+TGG + Chr6:23393486-23393505 None:intergenic 40.0%
!!! GATCTTGGATGTTGTTTTGG+AGG + Chr6:23393246-23393265 None:intergenic 40.0%
CTATGCTTTCCACCTTGTGA+TGG + Chr6:23393420-23393439 None:intergenic 45.0%
TAGTATAGGAGGAGGATCCT+TGG + Chr6:23393111-23393130 None:intergenic 45.0%
TGGTGTTCATCACCATCACA+AGG - Chr6:23393405-23393424 MsG0680031617.01.T01:CDS 45.0%
TGGTGTTCTTAAGCCAAGTC+TGG - Chr6:23393470-23393489 MsG0680031617.01.T01:CDS 45.0%
TGTTCATCACCATCACAAGG+TGG - Chr6:23393408-23393427 MsG0680031617.01.T01:CDS 45.0%
! GGAAAGCATAGAGCATGTCA+TGG - Chr6:23393429-23393448 MsG0680031617.01.T01:CDS 45.0%
!! GAGCTTTTGAGGAGAAATCG+TGG - Chr6:23393363-23393382 MsG0680031617.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr6 gene 23393057 23393566 23393057 ID=MsG0680031617.01;Name=MsG0680031617.01
Chr6 mRNA 23393057 23393566 23393057 ID=MsG0680031617.01.T01;Parent=MsG0680031617.01;Name=MsG0680031617.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|169
Chr6 exon 23393057 23393566 23393057 ID=MsG0680031617.01.T01:exon:14586;Parent=MsG0680031617.01.T01
Chr6 CDS 23393057 23393566 23393057 ID=MsG0680031617.01.T01:cds;Parent=MsG0680031617.01.T01
Gene Sequence

>MsG0680031617.01.T01

ATGCTATCAAGATCTTTTTCTGAGTATTGTATTCTTGGGTGGGAAAATTTACCAAGGATCCTCCTCCTATACTACACAAATTTTGTGTCATCTCCAGAAGGGTATTTCCAAACAGTTATATGCAATTCTAAAGACTACAAGAACACCACAGCTAACAATGATCTACACTACATTACTTGGGATAATCCTCCAAAACAACATCCAAGATCTCTAACACTCAAAGATTATAGGAAAATGGTTTTGAGCAACCGTCCATTTGCTAGAAAGTTTAAGAAAAATGATATTGTGCTTGATAAAATTGATAGAGAGCTTTTGAGGAGAAATCGTGGACAATTTTCTTTTGGAGGATGGTGTTCATCACCATCACAAGGTGGAAAGCATAGAGCATGTCATGGTTTGAAAATTGAGAATTATGGTGTTCTTAAGCCAAGTCTGGCCTCAAAAAAGATGAAAAATATGCTTTCAAATATTATTTCGTTTAAAAATTTACGCAAACAACAATGTCGGTAA

Protein sequence

>MsG0680031617.01.T01

MLSRSFSEYCILGWENLPRILLLYYTNFVSSPEGYFQTVICNSKDYKNTTANNDLHYITWDNPPKQHPRSLTLKDYRKMVLSNRPFARKFKKNDIVLDKIDRELLRRNRGQFSFGGWCSSPSQGGKHRACHGLKIENYGVLKPSLASKKMKNMLSNIISFKNLRKQQCR*