AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0144890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0144890 MtrunA17_Chr1g0153271 92.121 165 5 1 1 157 1 165 4.09e-104 305
Msa0144890 MtrunA17_Chr5g0402171 35.065 154 90 3 11 155 13 165 2.09e-21 89.4
Msa0144890 MtrunA17_Chr3g0123751 40.385 104 50 1 65 156 33 136 5.14e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0144890 AT1G56630.1 41.615 161 81 4 10 157 6 166 1.36e-29 112
Msa0144890 AT5G42930.2 38.994 159 86 2 10 157 6 164 2.00e-29 111
Msa0144890 AT5G42930.1 38.994 159 86 2 10 157 6 164 4.51e-29 110
Msa0144890 AT1G45201.2 40.152 132 74 2 30 157 39 169 5.87e-27 104
Msa0144890 AT1G45201.1 40.152 132 74 2 30 157 39 169 1.62e-26 103
Msa0144890 AT1G45201.3 40.152 132 74 2 30 157 64 194 1.82e-26 103
Msa0144890 AT5G67050.1 41.935 155 78 5 11 157 13 163 5.11e-25 99.8
Msa0144890 AT3G14360.1 26.203 187 104 5 2 154 10 196 1.49e-15 73.2

Find 31 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCCCTTCTTGAAAATTAT+TGG 0.135024 1_4:+4460866 Msa0144890:CDS
CTCTTCTTCAACTTTCTTAA+AGG 0.156900 1_4:+4460944 Msa0144890:CDS
ATCTCCATGTATTTGTTGTT+CGG 0.358677 1_4:-4460666 None:intergenic
TTCTTGCCTCAAGTCCTTGT+GGG 0.405312 1_4:-4460781 None:intergenic
TCAAGTAATGGAGGGCTCAT+TGG 0.410377 1_4:+4460920 Msa0144890:CDS
GTCGTCGGCGGAGTTCTTAT+CGG 0.410892 1_4:+4461129 Msa0144890:CDS
TTGGAGACATGTTGGAGTCT+TGG 0.414649 1_4:+4460885 Msa0144890:CDS
TAAGGCATTTCTTAAAGCTA+TGG 0.426559 1_4:+4460640 Msa0144890:intron
CTTAATTGTTTATCAAGTAA+TGG 0.429403 1_4:+4460908 Msa0144890:CDS
TGCATTGCAATGTAGACATA+AGG 0.439219 1_4:+4460510 None:intergenic
CAAGAAAATTTCGACAAAGA+TGG 0.458817 1_4:+4460798 Msa0144890:CDS
AAGGCATTTCTTAAAGCTAT+GGG 0.468246 1_4:+4460641 Msa0144890:intron
GAAAATTATTGGAGACATGT+TGG 0.474385 1_4:+4460877 Msa0144890:CDS
AAAGCAAGTGTTGTGGAACT+AGG 0.494529 1_4:+4460710 Msa0144890:CDS
ATAAGGATGTAAAGTACAAA+GGG 0.499364 1_4:+4461200 Msa0144890:CDS
GCGGAGTTCTTATCGGTGGT+TGG 0.509997 1_4:+4461136 Msa0144890:CDS
AGCTTCATTGATTGCCCACA+AGG 0.512388 1_4:+4460767 Msa0144890:CDS
CAAAGGGTTACTATCTATCA+TGG 0.525614 1_4:+4461216 Msa0144890:CDS
CATAAGGATGTAAAGTACAA+AGG 0.532579 1_4:+4461199 Msa0144890:CDS
TTTCTTGCCTCAAGTCCTTG+TGG 0.545498 1_4:-4460782 None:intergenic
AATTGTTTATCAAGTAATGG+AGG 0.560190 1_4:+4460911 Msa0144890:CDS
ATTGTTTATCAAGTAATGGA+GGG 0.563370 1_4:+4460912 Msa0144890:CDS
TGATTGCCCACAAGGACTTG+AGG 0.576965 1_4:+4460775 Msa0144890:CDS
CTAAATCAACTCTTGTGTCA+AGG 0.587660 1_4:-4461162 None:intergenic
ATGGAGATTATCTATTTCTG+AGG 0.588396 1_4:+4460681 Msa0144890:CDS
GGTTCCGAACAACAAATACA+TGG 0.590798 1_4:+4460662 Msa0144890:CDS
GTCGGCGGAGTTCTTATCGG+TGG 0.625309 1_4:+4461132 Msa0144890:CDS
AATCAACTCTTGTGTCAAGG+TGG 0.671807 1_4:-4461159 None:intergenic
GATAACACCGGAAAAGTCGT+CGG 0.682426 1_4:+4461114 Msa0144890:CDS
TGCAGGAGAGGTGATAACAC+CGG 0.684829 1_4:+4461102 Msa0144890:intron
AACACCGGAAAAGTCGTCGG+CGG 0.722840 1_4:+4461117 Msa0144890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTACATGAATTTAAATTT+TGG + chr1_4:4461076-4461095 Msa0144890:intron 15.0%
!!! GTTTACATGAATTTAAATTT+TGG + chr1_4:4461076-4461095 Msa0144890:intron 15.0%
!! CTTAATTGTTTATCAAGTAA+TGG + chr1_4:4460908-4460927 Msa0144890:CDS 20.0%
!!! TACATGAATTTAAATTTTGG+TGG + chr1_4:4461079-4461098 Msa0144890:intron 20.0%
!! AGACAAAAATATTCATCATA+AGG + chr1_4:4461183-4461202 Msa0144890:CDS 20.0%
!! CTTAATTGTTTATCAAGTAA+TGG + chr1_4:4460908-4460927 Msa0144890:CDS 20.0%
!!! TACATGAATTTAAATTTTGG+TGG + chr1_4:4461079-4461098 Msa0144890:intron 20.0%
!! AGACAAAAATATTCATCATA+AGG + chr1_4:4461183-4461202 Msa0144890:CDS 20.0%
!! CTTACAATTTTCTTTTGCTA+AGG + chr1_4:4460622-4460641 Msa0144890:intron 25.0%
!!! TCTCCAATAATTTTCAAGAA+GGG - chr1_4:4460872-4460891 None:intergenic 25.0%
! AATTGTTTATCAAGTAATGG+AGG + chr1_4:4460911-4460930 Msa0144890:CDS 25.0%
!! ATTGTTTATCAAGTAATGGA+GGG + chr1_4:4460912-4460931 Msa0144890:CDS 25.0%
! ATAAGGATGTAAAGTACAAA+GGG + chr1_4:4461200-4461219 Msa0144890:CDS 25.0%
!! CTTACAATTTTCTTTTGCTA+AGG + chr1_4:4460622-4460641 Msa0144890:intron 25.0%
!!! TCTCCAATAATTTTCAAGAA+GGG - chr1_4:4460872-4460891 None:intergenic 25.0%
! AATTGTTTATCAAGTAATGG+AGG + chr1_4:4460911-4460930 Msa0144890:CDS 25.0%
!! ATTGTTTATCAAGTAATGGA+GGG + chr1_4:4460912-4460931 Msa0144890:CDS 25.0%
! ATAAGGATGTAAAGTACAAA+GGG + chr1_4:4461200-4461219 Msa0144890:CDS 25.0%
! TAAGGCATTTCTTAAAGCTA+TGG + chr1_4:4460640-4460659 Msa0144890:intron 30.0%
! AAGGCATTTCTTAAAGCTAT+GGG + chr1_4:4460641-4460660 Msa0144890:intron 30.0%
!! ATCTCCATGTATTTGTTGTT+CGG - chr1_4:4460669-4460688 None:intergenic 30.0%
!! ATGGAGATTATCTATTTCTG+AGG + chr1_4:4460681-4460700 Msa0144890:CDS 30.0%
CTAAGTTTGATGAAAACAAG+AGG - chr1_4:4460739-4460758 None:intergenic 30.0%
CAAGAAAATTTCGACAAAGA+TGG + chr1_4:4460798-4460817 Msa0144890:CDS 30.0%
!! AATTTTCAAGAAGGGATCTA+TGG - chr1_4:4460864-4460883 None:intergenic 30.0%
GATCCCTTCTTGAAAATTAT+TGG + chr1_4:4460866-4460885 Msa0144890:CDS 30.0%
!! GTCTCCAATAATTTTCAAGA+AGG - chr1_4:4460873-4460892 None:intergenic 30.0%
GAAAATTATTGGAGACATGT+TGG + chr1_4:4460877-4460896 Msa0144890:CDS 30.0%
CTCTTCTTCAACTTTCTTAA+AGG + chr1_4:4460944-4460963 Msa0144890:CDS 30.0%
!!! AATTTAAATTTTGGTGGTGC+AGG + chr1_4:4461085-4461104 Msa0144890:intron 30.0%
CATAAGGATGTAAAGTACAA+AGG + chr1_4:4461199-4461218 Msa0144890:CDS 30.0%
! TAAGGCATTTCTTAAAGCTA+TGG + chr1_4:4460640-4460659 Msa0144890:intron 30.0%
! AAGGCATTTCTTAAAGCTAT+GGG + chr1_4:4460641-4460660 Msa0144890:intron 30.0%
!! ATCTCCATGTATTTGTTGTT+CGG - chr1_4:4460669-4460688 None:intergenic 30.0%
!! ATGGAGATTATCTATTTCTG+AGG + chr1_4:4460681-4460700 Msa0144890:CDS 30.0%
CTAAGTTTGATGAAAACAAG+AGG - chr1_4:4460739-4460758 None:intergenic 30.0%
CAAGAAAATTTCGACAAAGA+TGG + chr1_4:4460798-4460817 Msa0144890:CDS 30.0%
!! AATTTTCAAGAAGGGATCTA+TGG - chr1_4:4460864-4460883 None:intergenic 30.0%
GATCCCTTCTTGAAAATTAT+TGG + chr1_4:4460866-4460885 Msa0144890:CDS 30.0%
!! GTCTCCAATAATTTTCAAGA+AGG - chr1_4:4460873-4460892 None:intergenic 30.0%
GAAAATTATTGGAGACATGT+TGG + chr1_4:4460877-4460896 Msa0144890:CDS 30.0%
CTCTTCTTCAACTTTCTTAA+AGG + chr1_4:4460944-4460963 Msa0144890:CDS 30.0%
!!! AATTTAAATTTTGGTGGTGC+AGG + chr1_4:4461085-4461104 Msa0144890:intron 30.0%
CATAAGGATGTAAAGTACAA+AGG + chr1_4:4461199-4461218 Msa0144890:CDS 30.0%
CTAAATCAACTCTTGTGTCA+AGG - chr1_4:4461165-4461184 None:intergenic 35.0%
CAAAGGGTTACTATCTATCA+TGG + chr1_4:4461216-4461235 Msa0144890:CDS 35.0%
CTAAATCAACTCTTGTGTCA+AGG - chr1_4:4461165-4461184 None:intergenic 35.0%
CAAAGGGTTACTATCTATCA+TGG + chr1_4:4461216-4461235 Msa0144890:CDS 35.0%
GGTTCCGAACAACAAATACA+TGG + chr1_4:4460662-4460681 Msa0144890:CDS 40.0%
!!! TCCACAACACTTGCTTTTTG+TGG - chr1_4:4460707-4460726 None:intergenic 40.0%
! AAAGCAAGTGTTGTGGAACT+AGG + chr1_4:4460710-4460729 Msa0144890:CDS 40.0%
AATCAACTCTTGTGTCAAGG+TGG - chr1_4:4461162-4461181 None:intergenic 40.0%
GGTTCCGAACAACAAATACA+TGG + chr1_4:4460662-4460681 Msa0144890:CDS 40.0%
!!! TCCACAACACTTGCTTTTTG+TGG - chr1_4:4460707-4460726 None:intergenic 40.0%
! AAAGCAAGTGTTGTGGAACT+AGG + chr1_4:4460710-4460729 Msa0144890:CDS 40.0%
AATCAACTCTTGTGTCAAGG+TGG - chr1_4:4461162-4461181 None:intergenic 40.0%
! GCCACAAAAAGCAAGTGTTG+TGG + chr1_4:4460703-4460722 Msa0144890:CDS 45.0%
! AGCTTCATTGATTGCCCACA+AGG + chr1_4:4460767-4460786 Msa0144890:CDS 45.0%
TTCTTGCCTCAAGTCCTTGT+GGG - chr1_4:4460784-4460803 None:intergenic 45.0%
TTTCTTGCCTCAAGTCCTTG+TGG - chr1_4:4460785-4460804 None:intergenic 45.0%
TTGGAGACATGTTGGAGTCT+TGG + chr1_4:4460885-4460904 Msa0144890:CDS 45.0%
! TCAAGTAATGGAGGGCTCAT+TGG + chr1_4:4460920-4460939 Msa0144890:CDS 45.0%
!!! AAATTTTGGTGGTGCAGGAG+AGG + chr1_4:4461090-4461109 Msa0144890:intron 45.0%
GATAACACCGGAAAAGTCGT+CGG + chr1_4:4461114-4461133 Msa0144890:CDS 45.0%
! GCCACAAAAAGCAAGTGTTG+TGG + chr1_4:4460703-4460722 Msa0144890:CDS 45.0%
! AGCTTCATTGATTGCCCACA+AGG + chr1_4:4460767-4460786 Msa0144890:CDS 45.0%
TTCTTGCCTCAAGTCCTTGT+GGG - chr1_4:4460784-4460803 None:intergenic 45.0%
TTTCTTGCCTCAAGTCCTTG+TGG - chr1_4:4460785-4460804 None:intergenic 45.0%
TTGGAGACATGTTGGAGTCT+TGG + chr1_4:4460885-4460904 Msa0144890:CDS 45.0%
! TCAAGTAATGGAGGGCTCAT+TGG + chr1_4:4460920-4460939 Msa0144890:CDS 45.0%
!!! AAATTTTGGTGGTGCAGGAG+AGG + chr1_4:4461090-4461109 Msa0144890:intron 45.0%
GATAACACCGGAAAAGTCGT+CGG + chr1_4:4461114-4461133 Msa0144890:CDS 45.0%
TGATTGCCCACAAGGACTTG+AGG + chr1_4:4460775-4460794 Msa0144890:CDS 50.0%
TGCAGGAGAGGTGATAACAC+CGG + chr1_4:4461102-4461121 Msa0144890:intron 50.0%
TGATTGCCCACAAGGACTTG+AGG + chr1_4:4460775-4460794 Msa0144890:CDS 50.0%
TGCAGGAGAGGTGATAACAC+CGG + chr1_4:4461102-4461121 Msa0144890:intron 50.0%
AACACCGGAAAAGTCGTCGG+CGG + chr1_4:4461117-4461136 Msa0144890:CDS 55.0%
! AACTCCGCCGACGACTTTTC+CGG - chr1_4:4461124-4461143 None:intergenic 55.0%
GTCGTCGGCGGAGTTCTTAT+CGG + chr1_4:4461129-4461148 Msa0144890:CDS 55.0%
GCGGAGTTCTTATCGGTGGT+TGG + chr1_4:4461136-4461155 Msa0144890:CDS 55.0%
AACACCGGAAAAGTCGTCGG+CGG + chr1_4:4461117-4461136 Msa0144890:CDS 55.0%
! AACTCCGCCGACGACTTTTC+CGG - chr1_4:4461124-4461143 None:intergenic 55.0%
GTCGTCGGCGGAGTTCTTAT+CGG + chr1_4:4461129-4461148 Msa0144890:CDS 55.0%
GCGGAGTTCTTATCGGTGGT+TGG + chr1_4:4461136-4461155 Msa0144890:CDS 55.0%
GTCGGCGGAGTTCTTATCGG+TGG + chr1_4:4461132-4461151 Msa0144890:CDS 60.0%
GTCGGCGGAGTTCTTATCGG+TGG + chr1_4:4461132-4461151 Msa0144890:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 4460525 4461294 4460525 ID=Msa0144890;Name=Msa0144890
chr1_4 mRNA 4460525 4461294 4460525 ID=Msa0144890-mRNA-1;Parent=Msa0144890;Name=Msa0144890-mRNA-1;_AED=0.06;_eAED=0.06;_QI=22|0.33|0|0.75|0.33|0.25|4|0|157
chr1_4 exon 4460525 4460531 4460525 ID=Msa0144890-mRNA-1:exon:1871;Parent=Msa0144890-mRNA-1
chr1_4 exon 4460644 4460741 4460644 ID=Msa0144890-mRNA-1:exon:1872;Parent=Msa0144890-mRNA-1
chr1_4 exon 4460766 4460965 4460766 ID=Msa0144890-mRNA-1:exon:1873;Parent=Msa0144890-mRNA-1
chr1_4 exon 4461107 4461294 4461107 ID=Msa0144890-mRNA-1:exon:1874;Parent=Msa0144890-mRNA-1
chr1_4 five_prime_UTR 4460525 4460531 4460525 ID=Msa0144890-mRNA-1:five_prime_utr;Parent=Msa0144890-mRNA-1
chr1_4 five_prime_UTR 4460644 4460658 4460644 ID=Msa0144890-mRNA-1:five_prime_utr;Parent=Msa0144890-mRNA-1
chr1_4 CDS 4460659 4460741 4460659 ID=Msa0144890-mRNA-1:cds;Parent=Msa0144890-mRNA-1
chr1_4 CDS 4460766 4460965 4460766 ID=Msa0144890-mRNA-1:cds;Parent=Msa0144890-mRNA-1
chr1_4 CDS 4461107 4461294 4461107 ID=Msa0144890-mRNA-1:cds;Parent=Msa0144890-mRNA-1
Gene Sequence

>Msa0144890

ATGGGTTCCGAACAACAAATACATGGAGATTATCTATTTCTGAGGCCACAAAAAGCAAGTGTTGTGGAACTAGGAAGCCTCTTAAGCTTCATTGATTGCCCACAAGGACTTGAGGCAAGAAAATTTCGACAAAGATGGCTTCTCTTTACTTCAGTTGTTGCACAAATTGTTCTTGTTGCCATAGATCCCTTCTTGAAAATTATTGGAGACATGTTGGAGTCTTGGCTTAATTGTTTATCAAGTAATGGAGGGCTCATTGGACTCTTCTTCAACTTTCTTAAAGGAGAGGTGATAACACCGGAAAAGTCGTCGGCGGAGTTCTTATCGGTGGTTGGCCACCTTGACACAAGAGTTGATTTAGACAAAAATATTCATCATAAGGATGTAAAGTACAAAGGGTTACTATCTATCATGGCTTCTAAATTTTCGTACGAGAATGAACAAGTCATTAGCAATGCAGTTAGAAATAAT

Protein sequence

>Msa0144890

MGSEQQIHGDYLFLRPQKASVVELGSLLSFIDCPQGLEARKFRQRWLLFTSVVAQIVLVAIDPFLKIIGDMLESWLNCLSSNGGLIGLFFNFLKGEVITPEKSSAEFLSVVGHLDTRVDLDKNIHHKDVKYKGLLSIMASKFSYENEQVISNAVRNN