AlfalfaGEDB | Msa1177650

M. sativa cultivar zhongmu-4 / Msa1177650

KEGG: K13425
GO: GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0009889 GO:0010033 GO:0010150 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090693 GO:0099402 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1177650	sp\|O04609\|WRK22_ARATH	46.273	322	125	9	1	315	1	281	1.63e-75	237

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1177650	A0A072TPN3	94.675	338	16	2	1	336	1	338	1.84e-175	499

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TFs/TRs:

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Gene ID	Type	Classification
Msa1177650	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa1177650	-0.845665	3.525041e-59	-8.615850e-47
Msa0000800	Msa1177650	0.842942	1.899668e-58	-8.615850e-47
Msa0001510	Msa1177650	-0.834680	2.605818e-56	-8.615850e-47
Msa0002110	Msa1177650	-0.848150	7.362045e-60	-8.615850e-47
Msa0002690	Msa1177650	-0.847474	1.130637e-59	-8.615850e-47
Msa0002700	Msa1177650	-0.840441	8.678103e-58	-8.615850e-47
Msa0003340	Msa1177650	-0.841283	5.220737e-58	-8.615850e-47
Msa0004180	Msa1177650	-0.878062	3.804378e-69	-8.615850e-47
Msa0006080	Msa1177650	0.833580	4.914206e-56	-8.615850e-47
Msa0006480	Msa1177650	0.811134	8.148796e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1177650	MtrunA17_Chr8g0348311	94.675	338	16	2	1	336	1	338	3.54e-179	499
Msa1177650	MtrunA17_Chr7g0248691	54.678	342	114	9	1	331	1	312	9.62e-104	307
Msa1177650	MtrunA17_Chr2g0293481	39.051	274	118	7	95	324	191	459	2.98e-48	169
Msa1177650	MtrunA17_Chr1g0202461	67.544	114	34	2	130	242	237	348	1.79e-46	162
Msa1177650	MtrunA17_Chr8g0387501	40.323	248	115	8	69	310	49	269	5.55e-39	139
Msa1177650	MtrunA17_Chr7g0270051	54.310	116	39	3	133	237	24	136	5.41e-35	128
Msa1177650	MtrunA17_Chr2g0284321	57.732	97	34	2	127	217	4	99	1.54e-32	120
Msa1177650	MtrunA17_Chr8g0379461	53.608	97	32	3	134	218	46	141	1.33e-28	110
Msa1177650	MtrunA17_Chr5g0401821	59.036	83	29	1	133	210	174	256	5.11e-28	110
Msa1177650	MtrunA17_Chr2g0319961	55.682	88	33	1	132	213	53	140	1.07e-27	108

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1177650	AT4G01250.1	46.273	322	125	9	1	315	1	281	1.66e-76	237
Msa1177650	AT5G52830.1	56.863	153	51	4	132	281	143	283	3.77e-49	168
Msa1177650	AT1G30650.1	46.047	215	84	7	134	322	188	396	7.95e-44	156
Msa1177650	AT2G34830.1	45.320	203	81	4	134	306	186	388	8.88e-42	150
Msa1177650	AT2G34830.2	45.320	203	81	4	134	306	195	397	9.52e-42	150
Msa1177650	AT4G23550.1	43.590	234	98	7	6	231	11	218	1.39e-41	147
Msa1177650	AT5G45050.1	61.290	93	33	1	132	221	1156	1248	2.77e-33	130
Msa1177650	AT5G45050.2	61.290	93	33	1	132	221	1128	1220	3.09e-33	130
Msa1177650	AT5G45050.4	61.290	93	33	1	132	221	1017	1109	3.35e-33	130
Msa1177650	AT1G29280.1	50.427	117	41	3	120	225	31	141	1.61e-32	122

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Find 87 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGTAGCATCATCTGG+TGG	0.715149	8_1:-10027458	Msa1177650:CDS
AACGGTTATGGAGGTAGATT+GGG	0.399198	8_1:-10029069	Msa1177650:exon
AACTAACAATGCTATGTGAA+GGG	0.504246	8_1:-10027052	Msa1177650:three_prime_UTR
AAGTTCATGCAATTCTTCAA+TGG	0.450245	8_1:+10028848	None:intergenic
AATGATGAATGATGATGATG+AGG	0.613568	8_1:-10027561	Msa1177650:CDS
AATTTCTGTGAGTTGGAGCA+GGG	0.599997	8_1:+10027756	None:intergenic
ACAATTTCAATTTAAAGTGT+CGG	0.492795	8_1:-10027225	Msa1177650:three_prime_UTR
ACACGATCCTTATGTCTGAC+AGG	0.564606	8_1:-10027352	Msa1177650:three_prime_UTR
ACTAACAATGCTATGTGAAG+GGG	0.600258	8_1:-10027051	Msa1177650:three_prime_UTR
ACTCACAGAAATTCTCTTGC+TGG	0.378623	8_1:-10027746	Msa1177650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTCATGCAATTCTTCAA+TGG	+	chr8_1:10027263-10027282	None:intergenic	30.0%
TATGGTCAAAAACCAATCAA+AGG	-	chr8_1:10027777-10027796	Msa1177650:CDS	30.0%
ATGTTCATAGTTACATACAC+TGG	-	chr8_1:10028317-10028336	Msa1177650:CDS	30.0%
GTAACAGATGATTTCTTTGA+AGG	-	chr8_1:10028599-10028618	Msa1177650:intron	30.0%
TCTAATCCCTATTCTCTATA+AGG	+	chr8_1:10028966-10028985	None:intergenic	30.0%
TTAGAGGAATTGGTACATTA+TGG	-	chr8_1:10029024-10029043	Msa1177650:CDS	30.0%
TAACTAACAATGCTATGTGA+AGG	-	chr8_1:10029055-10029074	Msa1177650:CDS	30.0%
AACTAACAATGCTATGTGAA+GGG	-	chr8_1:10029056-10029075	Msa1177650:CDS	30.0%
AAGTTCATGCAATTCTTCAA+TGG	+	chr8_1:10027263-10027282	None:intergenic	30.0%
TATGGTCAAAAACCAATCAA+AGG	-	chr8_1:10027777-10027796	Msa1177650:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	10026933	10029197	10026933	ID=Msa1177650;Name=Msa1177650
chr8_1	mRNA	10026933	10029197	10026933	ID=Msa1177650-mRNA-1;Parent=Msa1177650;Name=Msa1177650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=113\|1\|1\|1\|1\|1\|3\|464\|336
chr8_1	exon	10026933	10027889	10026933	ID=Msa1177650-mRNA-1:exon:3469;Parent=Msa1177650-mRNA-1
chr8_1	exon	10028316	10028426	10028316	ID=Msa1177650-mRNA-1:exon:3468;Parent=Msa1177650-mRNA-1
chr8_1	exon	10028678	10029197	10028678	ID=Msa1177650-mRNA-1:exon:3467;Parent=Msa1177650-mRNA-1
chr8_1	five_prime_UTR	10029085	10029197	10029085	ID=Msa1177650-mRNA-1:five_prime_utr;Parent=Msa1177650-mRNA-1
chr8_1	CDS	10028678	10029084	10028678	ID=Msa1177650-mRNA-1:cds;Parent=Msa1177650-mRNA-1
chr8_1	CDS	10028316	10028426	10028316	ID=Msa1177650-mRNA-1:cds;Parent=Msa1177650-mRNA-1
chr8_1	CDS	10027397	10027889	10027397	ID=Msa1177650-mRNA-1:cds;Parent=Msa1177650-mRNA-1
chr8_1	three_prime_UTR	10026933	10027396	10026933	ID=Msa1177650-mRNA-1:three_prime_utr;Parent=Msa1177650-mRNA-1

Gene Sequence

>Msa1177650

ATGGAGGTAGATTGGGATCTACATGCTGTAGTCAGAGGCTATACCAACCTCACCTCCTCCTCTTCCTCCTCCTCCACCACCTCAACAACCACCTTAGCCACCTCATCAACACCTTCTTCAACATCTTATAGTAATAATAACTTTTTCTCTATTTACCCTACAGAACAAACAAGTCAAGTTTTTTCACTTTCAAACCCCTTTGAAACTAAAAGTTCCATTGAAGAATTGCATGAACTTTGCAAACCTTTCTTATTCAAATCATCATCATCATCACAACCTCTCTCTTCAACTTCCTTCTCTTATTCATCACCATCACCAAAATCACCTCACATACAACCAAAACAGCTTCTGGTTAATAACAAGCTTCATCATGCTGCTTCTGCCATCACACCAAGATCCAAAAGAAGGAAGATTCAGCATAAGAAGGTTTGTGAAGTGCAAGCTGAGAAGCTCTCATCAGACATATGGGCATGGAGGAAATATGGTCAAAAACCAATCAAAGGCTCACCATATCCAAGGGGATATTACAGATGTAGCAGTTCAAAAGGATGTTCAGCTAAGAAACAAGTTGAGAGGAACAAATCAGACCCATCAATGTTCATAGTTACATACACTGGTGAACACAGTCACCCTGCTCCAACTCACAGAAATTCTCTTGCTGGTTCAACAAGACAGAAGCCATTGTCACCACAAACTGACACTGCTGAAGACTCTTCCCAACAACCTTTTACAAAACAAGTTTCATCATCCACATCAGAAGCTGAAGAGGAAGAGGCTACAACACTCTTAAGTGTAAAGTCAGAGAGCAAAGATGATTTGGAAGAAATGATGAATGATGATGATGAGGAAAATGAATTTGAGTTATCTGATATGGTTGTAACAGATGATTTCTTTGAAGGTTTAGATGAATTGACAGGTTTTGCTGGAAAAACAGTAGCATCATCTGGTGGTGACTGTTTTGGTGACCCTTTTGCTGCTAGTATTGCTCTTCCTGCTTGTGGTGGCCAATGA

Protein sequence

>Msa1177650

MEVDWDLHAVVRGYTNLTSSSSSSSTTSTTTLATSSTPSSTSYSNNNFFSIYPTEQTSQVFSLSNPFETKSSIEELHELCKPFLFKSSSSSQPLSSTSFSYSSPSPKSPHIQPKQLLVNNKLHHAASAITPRSKRRKIQHKKVCEVQAEKLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSAKKQVERNKSDPSMFIVTYTGEHSHPAPTHRNSLAGSTRQKPLSPQTDTAEDSSQQPFTKQVSSSTSEAEEEEATTLLSVKSESKDDLEEMMNDDDEENEFELSDMVVTDDFFEGLDELTGFAGKTVASSGGDCFGDPFAASIALPACGGQ*