AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0319220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0319220 MtrunA17_Chr2g0293481 96.701 485 6 4 1 477 1 483 0.0 949
Msa0319220 MtrunA17_Chr7g0248691 49.479 192 70 5 232 423 135 299 4.98e-48 168
Msa0319220 MtrunA17_Chr8g0348311 41.892 222 98 4 232 453 136 326 2.78e-44 158
Msa0319220 MtrunA17_Chr7g0270051 42.449 245 103 11 210 423 4 241 1.00e-39 144
Msa0319220 MtrunA17_Chr8g0379461 65.625 96 31 1 232 325 45 140 1.15e-37 138
Msa0319220 MtrunA17_Chr1g0202461 61.165 103 31 1 232 334 241 334 7.55e-37 139
Msa0319220 MtrunA17_Chr2g0319961 55.556 117 46 2 215 325 28 144 2.73e-35 132
Msa0319220 MtrunA17_Chr8g0387501 68.235 85 23 1 261 345 129 209 1.45e-34 130
Msa0319220 MtrunA17_Chr2g0284321 53.279 122 47 2 233 354 11 122 8.87e-33 124
Msa0319220 MtrunA17_Chr4g0071521 59.770 87 30 1 232 318 221 302 2.56e-29 117
Msa0319220 MtrunA17_Chr1g0147881 57.955 88 32 2 231 318 237 319 3.48e-29 117
Msa0319220 MtrunA17_Chr5g0401821 56.842 95 36 2 224 318 167 256 1.12e-27 111
Msa0319220 MtrunA17_Chr4g0053281 57.471 87 32 2 232 318 242 323 4.74e-26 108
Msa0319220 MtrunA17_Chr1g0184691 53.846 91 36 3 234 323 271 356 7.09e-25 105
Msa0319220 MtrunA17_Chr7g0228941 63.333 60 22 0 259 318 50 109 1.09e-24 99.0
Msa0319220 MtrunA17_Chr3g0139021 48.454 97 45 2 222 318 280 371 1.83e-24 105
Msa0319220 MtrunA17_Chr2g0289181 51.948 77 36 1 259 334 12 88 2.17e-23 98.6
Msa0319220 MtrunA17_Chr1g0194481 52.632 76 34 2 262 336 159 233 2.83e-20 91.7
Msa0319220 MtrunA17_Chr7g0270831 52.113 71 34 0 261 331 216 286 6.84e-19 89.0
Msa0319220 MtrunA17_Chr3g0099641 41.584 101 58 1 236 336 227 326 8.44e-19 89.0
Msa0319220 MtrunA17_Chr4g0051881 57.377 61 25 1 262 322 179 238 9.39e-19 87.4
Msa0319220 MtrunA17_Chr7g0218081 51.316 76 36 1 262 337 411 485 1.21e-18 88.6
Msa0319220 MtrunA17_Chr4g0042831 41.584 101 52 2 230 324 241 340 5.69e-18 85.5
Msa0319220 MtrunA17_Chr4g0001361 49.367 79 39 1 255 333 157 234 6.23e-18 84.7
Msa0319220 MtrunA17_Chr2g0288871 57.377 61 26 0 261 321 305 365 9.66e-18 85.9
Msa0319220 MtrunA17_Chr4g0025061 30.058 173 96 4 160 331 282 430 3.48e-17 84.3
Msa0319220 MtrunA17_Chr5g0418191 47.561 82 39 2 262 340 321 401 4.14e-17 83.6
Msa0319220 MtrunA17_Chr5g0418191 48.333 60 29 2 262 321 152 209 7.96e-11 63.9
Msa0319220 MtrunA17_Chr5g0443131 55.738 61 26 1 262 322 158 217 4.60e-17 82.0
Msa0319220 MtrunA17_Chr7g0270191 39.259 135 70 5 222 349 109 238 8.51e-17 80.9
Msa0319220 MtrunA17_Chr4g0051181 54.098 61 28 0 262 322 244 304 1.19e-16 82.4
Msa0319220 MtrunA17_Chr2g0333851 43.038 79 45 0 258 336 166 244 1.27e-16 80.9
Msa0319220 MtrunA17_Chr7g0216891 48.571 70 36 0 262 331 220 289 1.49e-16 82.4
Msa0319220 MtrunA17_Chr7g0238921 43.299 97 48 2 222 318 91 180 1.78e-16 80.1
Msa0319220 MtrunA17_Chr4g0025051 45.333 75 41 0 257 331 331 405 1.84e-16 82.0
Msa0319220 MtrunA17_Chr4g0075201 53.333 60 28 0 262 321 268 327 2.08e-16 81.6
Msa0319220 MtrunA17_Chr7g0263531 55.385 65 28 1 259 323 359 422 4.56e-16 80.1
Msa0319220 MtrunA17_Chr7g0263531 39.796 98 46 4 243 331 164 257 2.70e-12 68.6
Msa0319220 MtrunA17_Chr7g0249311 40.000 100 53 2 232 325 357 455 4.74e-16 80.9
Msa0319220 MtrunA17_Chr7g0249311 47.143 70 35 2 252 321 170 237 1.48e-11 66.6
Msa0319220 MtrunA17_Chr2g0302391 39.394 99 59 1 235 333 186 283 1.23e-15 79.0
Msa0319220 MtrunA17_Chr5g0418781 54.386 57 25 1 262 318 125 180 1.47e-15 77.0
Msa0319220 MtrunA17_Chr3g0101131 45.000 80 34 2 262 332 416 494 2.19e-15 78.6
Msa0319220 MtrunA17_Chr2g0322391 45.833 72 39 0 262 333 350 421 2.47e-15 78.6
Msa0319220 MtrunA17_Chr2g0294231 51.667 60 28 1 262 321 204 262 2.81e-15 77.4
Msa0319220 MtrunA17_Chr8g0383251 40.217 92 47 3 230 321 84 167 3.16e-15 73.9
Msa0319220 MtrunA17_Chr3g0136961 42.529 87 38 1 262 348 139 213 3.80e-15 75.9
Msa0319220 MtrunA17_Chr3g0129321 35.537 121 74 3 204 322 60 178 3.90e-15 74.3
Msa0319220 MtrunA17_Chr5g0445311 47.692 65 33 1 262 326 365 428 4.15e-15 77.8
Msa0319220 MtrunA17_Chr8g0347251 31.250 224 111 10 173 386 54 244 7.58e-15 75.9
Msa0319220 MtrunA17_Chr3g0126391 42.342 111 56 4 262 369 134 239 1.11e-14 75.1
Msa0319220 MtrunA17_Chr4g0060271 51.667 60 29 0 262 321 256 315 1.25e-14 76.3
Msa0319220 MtrunA17_Chr1g0150281 42.857 77 39 1 242 318 126 197 1.79e-14 73.9
Msa0319220 MtrunA17_Chr4g0068541 42.667 75 42 1 262 336 209 282 2.77e-14 74.7
Msa0319220 MtrunA17_Chr7g0249301 41.975 81 46 1 244 324 275 354 3.72e-14 73.9
Msa0319220 MtrunA17_Chr7g0249301 50.000 70 33 2 252 321 53 120 7.45e-13 70.1
Msa0319220 MtrunA17_Chr4g0024391 41.333 75 44 0 257 331 225 299 4.51e-14 74.3
Msa0319220 MtrunA17_Chr3g0079691 46.575 73 37 2 255 326 488 559 4.81e-14 74.7
Msa0319220 MtrunA17_Chr3g0079691 49.296 71 33 3 262 331 281 349 5.19e-12 68.2
Msa0319220 MtrunA17_Chr7g0275531 39.796 98 45 3 262 353 119 208 5.61e-14 72.8
Msa0319220 MtrunA17_Chr4g0004911 46.479 71 36 2 255 324 329 398 6.70e-14 73.9
Msa0319220 MtrunA17_Chr4g0004911 49.254 67 31 2 262 326 185 250 1.67e-12 69.3
Msa0319220 MtrunA17_Chr8g0335811 33.065 124 73 3 195 318 97 210 7.25e-14 70.9
Msa0319220 MtrunA17_Chr7g0224801 45.902 61 32 1 259 319 91 150 8.32e-14 69.3
Msa0319220 MtrunA17_Chr6g0485431 44.156 77 41 2 246 321 139 214 9.52e-14 72.4
Msa0319220 MtrunA17_Chr4g0074001 47.761 67 32 2 262 326 360 425 2.32e-13 72.0
Msa0319220 MtrunA17_Chr5g0432161 37.864 103 55 2 232 326 384 485 2.49e-13 72.4
Msa0319220 MtrunA17_Chr1g0150801 34.862 109 66 3 211 319 56 159 2.64e-13 67.8
Msa0319220 MtrunA17_Chr5g0447701 48.485 66 33 1 262 327 253 317 4.20e-13 70.9
Msa0319220 MtrunA17_Chr1g0188941 45.312 64 34 1 259 322 49 111 4.75e-13 65.5
Msa0319220 MtrunA17_Chr3g0106491 46.154 65 34 1 262 326 366 429 8.35e-13 70.5
Msa0319220 MtrunA17_Chr8g0383351 46.667 60 31 1 259 318 158 216 9.23e-13 67.8
Msa0319220 MtrunA17_Chr7g0243891 42.667 75 41 2 255 328 367 440 9.28e-13 70.5
Msa0319220 MtrunA17_Chr7g0243891 38.281 128 63 6 262 379 217 338 8.01e-11 64.3
Msa0319220 MtrunA17_Chr1g0150271 42.424 66 38 0 258 323 98 163 9.54e-13 68.2
Msa0319220 MtrunA17_Chr3g0129361 46.667 60 31 1 259 318 142 200 1.79e-12 66.6
Msa0319220 MtrunA17_Chr1g0189391 37.234 94 51 3 232 318 62 154 4.87e-12 67.0
Msa0319220 MtrunA17_Chr7g0248721 41.573 89 47 5 232 319 113 197 6.62e-12 65.1
Msa0319220 MtrunA17_Chr8g0387981 50.877 57 26 1 262 316 133 189 2.70e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0319220 AT1G30650.1 41.176 493 211 15 1 477 1 430 1.14e-86 273
Msa0319220 AT2G34830.2 40.977 471 184 18 1 438 1 410 7.26e-82 260
Msa0319220 AT2G34830.1 40.998 461 179 17 11 438 1 401 7.30e-80 255
Msa0319220 AT4G01250.1 48.603 179 61 4 232 402 109 264 1.07e-44 159
Msa0319220 AT5G52830.1 52.667 150 37 3 232 381 145 260 2.00e-40 149
Msa0319220 AT5G45050.1 40.927 259 108 9 210 451 1141 1371 7.01e-40 154
Msa0319220 AT5G45050.2 40.927 259 108 9 210 451 1113 1343 8.30e-40 154
Msa0319220 AT1G29280.1 60.526 114 39 2 220 332 32 140 8.93e-40 144
Msa0319220 AT5G45050.4 40.927 259 108 9 210 451 1002 1232 9.12e-40 154
Msa0319220 AT3G58710.2 68.478 92 28 1 232 322 40 131 4.96e-38 140
Msa0319220 AT3G58710.1 66.327 98 32 1 226 322 35 132 5.82e-38 140
Msa0319220 AT4G23550.1 56.911 123 43 2 236 357 124 237 1.24e-34 132
Msa0319220 AT2G30590.1 54.455 101 40 3 224 323 281 376 9.30e-29 117
Msa0319220 AT5G45260.1 57.009 107 33 2 232 336 1190 1285 2.08e-28 120
Msa0319220 AT2G23320.1 46.957 115 56 1 204 318 188 297 2.40e-27 112
Msa0319220 AT4G31550.2 55.172 87 34 1 232 318 221 302 4.76e-27 111
Msa0319220 AT4G24240.1 53.191 94 39 1 225 318 250 338 1.27e-26 110
Msa0319220 AT4G31550.1 55.172 87 34 1 232 318 222 303 1.58e-26 110
Msa0319220 AT2G24570.1 54.023 87 35 1 232 318 219 300 2.90e-25 106
Msa0319220 AT5G28650.1 52.874 87 36 2 232 318 238 319 1.07e-23 102
Msa0319220 AT3G04670.1 52.874 87 36 2 232 318 238 319 1.24e-23 102
Msa0319220 AT5G46350.1 54.054 74 31 2 262 333 184 256 4.65e-20 91.3
Msa0319220 AT1G29860.1 53.623 69 30 2 262 329 137 204 1.23e-18 86.7
Msa0319220 AT4G18170.1 57.377 61 25 1 262 322 173 232 2.28e-18 86.3
Msa0319220 AT1G68150.1 48.649 74 38 0 262 335 236 309 3.24e-18 86.7
Msa0319220 AT1G13960.2 45.000 100 42 4 232 321 219 315 5.44e-18 85.9
Msa0319220 AT1G13960.2 50.000 60 28 2 262 321 77 134 2.72e-11 65.5
Msa0319220 AT1G13960.1 45.000 100 42 4 232 321 372 468 8.09e-18 86.7
Msa0319220 AT1G13960.1 50.000 60 28 2 262 321 230 287 3.67e-11 65.9
Msa0319220 AT4G04450.2 50.704 71 35 0 261 331 198 268 1.38e-17 85.5
Msa0319220 AT5G49520.1 55.000 60 26 1 262 321 222 280 1.55e-17 84.7
Msa0319220 AT4G04450.1 48.684 76 39 0 261 336 292 367 1.59e-17 85.5
Msa0319220 AT1G62300.1 55.738 61 27 0 261 321 312 372 1.76e-17 85.5
Msa0319220 AT4G22070.4 55.738 61 27 0 261 321 297 357 3.09e-17 84.7
Msa0319220 AT4G22070.3 55.738 61 27 0 261 321 297 357 3.09e-17 84.7
Msa0319220 AT4G22070.2 55.738 61 27 0 261 321 297 357 3.09e-17 84.7
Msa0319220 AT4G22070.1 55.738 61 27 0 261 321 297 357 3.09e-17 84.7
Msa0319220 AT2G04880.2 54.545 66 29 1 259 324 281 345 3.90e-17 84.0
Msa0319220 AT2G04880.1 54.545 66 29 1 259 324 305 369 3.99e-17 84.3
Msa0319220 AT2G03340.1 47.778 90 42 3 262 350 416 501 6.00e-17 84.0
Msa0319220 AT2G03340.1 41.304 92 52 2 262 353 251 340 7.29e-13 71.2
Msa0319220 AT1G80840.1 48.485 66 34 0 258 323 143 208 7.97e-17 81.6
Msa0319220 AT3G62340.1 53.333 60 27 1 262 321 119 177 2.65e-16 79.7
Msa0319220 AT4G01720.1 39.000 100 60 1 222 321 201 299 3.22e-16 81.3
Msa0319220 AT5G26170.1 47.887 71 35 2 248 318 101 169 1.00e-15 75.5
Msa0319220 AT5G15130.1 47.143 70 37 0 262 331 228 297 1.30e-15 79.7
Msa0319220 AT5G15130.2 47.143 70 37 0 262 331 186 255 1.46e-15 79.3
Msa0319220 AT1G18860.1 53.333 60 28 0 262 321 240 299 1.88e-15 79.3
Msa0319220 AT2G21900.1 37.363 91 53 2 232 322 83 169 2.07e-15 75.5
Msa0319220 AT4G31800.2 47.945 73 35 1 262 334 112 181 3.31e-15 75.9
Msa0319220 AT1G69310.4 52.632 57 26 1 262 318 148 203 3.64e-15 76.3
Msa0319220 AT1G69310.2 52.632 57 26 1 262 318 148 203 3.64e-15 76.3
Msa0319220 AT1G69310.1 52.632 57 26 1 262 318 148 203 3.64e-15 76.3
Msa0319220 AT2G25000.2 51.667 60 29 0 262 321 104 163 6.03e-15 74.7
Msa0319220 AT2G25000.4 51.667 60 29 0 262 321 104 163 6.03e-15 74.7
Msa0319220 AT4G31800.1 47.945 73 35 1 262 334 177 246 6.18e-15 75.9
Msa0319220 AT2G25000.3 51.667 60 29 0 262 321 126 185 8.96e-15 74.7
Msa0319220 AT5G07100.2 46.053 76 36 2 262 333 142 216 1.06e-14 73.6
Msa0319220 AT5G07100.4 46.053 76 36 2 262 333 142 216 1.06e-14 73.6
Msa0319220 AT5G07100.5 46.053 76 36 2 262 333 142 216 1.06e-14 73.6
Msa0319220 AT5G07100.3 46.053 76 36 2 262 333 142 216 1.06e-14 73.6
Msa0319220 AT2G47260.1 51.667 60 28 1 262 321 175 233 1.43e-14 75.1
Msa0319220 AT2G25000.1 51.667 60 29 0 262 321 147 206 1.63e-14 74.3
Msa0319220 AT5G41570.1 40.000 90 48 2 230 319 72 155 2.07e-14 72.0
Msa0319220 AT5G07100.1 46.053 76 36 2 262 333 235 309 2.08e-14 74.3
Msa0319220 AT1G64000.1 50.820 61 29 1 259 319 112 171 2.55e-14 72.0
Msa0319220 AT1G69810.1 38.462 104 50 3 229 331 184 274 3.00e-14 74.7
Msa0319220 AT4G26640.5 46.154 65 34 1 262 326 206 269 4.09e-14 74.3
Msa0319220 AT4G26640.6 46.154 65 34 1 262 326 206 269 4.09e-14 74.3
Msa0319220 AT4G26640.4 46.154 65 34 1 262 326 206 269 4.09e-14 74.3
Msa0319220 AT4G26640.3 46.154 65 34 1 262 326 206 269 4.09e-14 74.3
Msa0319220 AT4G26640.7 46.154 65 34 1 262 326 342 405 5.65e-14 74.7
Msa0319220 AT1G69810.2 38.462 104 50 3 229 331 126 216 5.72e-14 73.2
Msa0319220 AT4G26640.2 46.154 65 34 1 262 326 382 445 6.12e-14 74.7
Msa0319220 AT4G26640.1 46.154 65 34 1 262 326 310 373 6.18e-14 74.3
Msa0319220 AT4G30935.1 47.761 67 32 2 262 326 332 397 7.50e-14 73.9
Msa0319220 AT3G01080.2 50.000 60 29 1 262 321 345 403 8.98e-14 73.6
Msa0319220 AT3G01080.2 47.059 68 34 2 262 329 206 271 3.25e-12 68.9
Msa0319220 AT3G01080.1 50.000 60 29 1 262 321 307 365 9.17e-14 73.6
Msa0319220 AT3G01080.1 47.059 68 34 2 262 329 168 233 3.30e-12 68.6
Msa0319220 AT2G37260.3 54.386 57 25 1 262 318 270 325 9.97e-14 72.8
Msa0319220 AT2G37260.3 28.426 197 119 5 255 432 79 272 5.32e-11 64.3
Msa0319220 AT2G37260.2 54.386 57 25 1 262 318 268 323 1.01e-13 72.8
Msa0319220 AT2G37260.2 28.426 197 119 5 255 432 77 270 4.98e-11 64.7
Msa0319220 AT2G37260.1 54.386 57 25 1 262 318 350 405 1.04e-13 73.6
Msa0319220 AT2G37260.1 28.426 197 119 5 255 432 159 352 5.44e-11 65.1
Msa0319220 AT5G56270.1 49.206 63 31 1 262 324 488 549 1.05e-13 73.9
Msa0319220 AT5G56270.1 47.059 68 32 3 262 327 274 339 1.51e-11 67.4
Msa0319220 AT5G13080.1 45.902 61 32 1 259 319 65 124 1.12e-13 68.9
Msa0319220 AT2G38470.1 44.444 72 39 1 262 333 363 433 1.25e-13 73.6
Msa0319220 AT4G39410.1 36.458 96 53 2 223 318 192 279 1.32e-13 72.0
Msa0319220 AT2G40750.1 33.099 142 84 4 232 367 122 258 4.42e-13 70.9
Msa0319220 AT2G44745.1 38.462 91 55 1 228 318 112 201 5.01e-13 68.9
Msa0319220 AT2G30250.1 50.820 61 29 1 262 322 329 388 6.07e-13 70.9
Msa0319220 AT4G26440.1 44.118 68 37 1 259 326 370 436 9.43e-13 70.9
Msa0319220 AT4G26440.1 46.667 75 36 3 262 334 179 251 1.43e-12 70.1
Msa0319220 AT3G01970.1 45.000 60 32 1 259 318 63 121 1.03e-12 66.2
Msa0319220 AT2G40740.1 40.909 88 45 3 232 316 147 230 1.19e-12 68.9
Msa0319220 AT2G46130.1 45.902 61 32 1 259 319 28 87 1.37e-12 64.7
Msa0319220 AT1G55600.1 46.774 62 32 1 260 321 306 366 1.41e-12 70.1
Msa0319220 AT5G43290.1 35.811 148 88 5 262 407 115 257 1.44e-12 68.6
Msa0319220 AT2G40740.3 40.909 88 45 3 232 316 156 239 1.54e-12 68.6
Msa0319220 AT5G24110.1 46.575 73 32 2 246 316 103 170 2.02e-12 68.6
Msa0319220 AT3G01080.3 47.059 68 34 2 262 329 206 271 2.21e-12 68.6
Msa0319220 AT3G56400.1 30.159 126 78 2 201 316 52 177 4.52e-12 67.0
Msa0319220 AT4G11070.2 49.123 57 27 1 262 316 110 166 6.00e-12 66.6
Msa0319220 AT4G11070.1 44.928 69 32 2 262 324 142 210 6.04e-12 67.0
Msa0319220 AT1G66550.2 33.684 95 61 1 224 316 66 160 7.67e-12 65.9
Msa0319220 AT5G64810.1 46.667 60 31 1 259 318 108 166 1.31e-11 63.9
Msa0319220 AT2G46400.1 39.583 96 46 4 226 316 73 161 1.36e-11 65.9
Msa0319220 AT1G66560.1 49.153 59 28 1 260 316 102 160 1.93e-11 64.7
Msa0319220 AT4G23810.1 49.123 57 27 1 262 316 159 215 3.85e-11 64.7
Msa0319220 AT1G66550.1 32.941 85 55 1 234 316 81 165 6.22e-11 63.2
Msa0319220 AT1G80590.1 32.540 126 78 2 262 385 86 206 9.24e-11 62.4

Find 103 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAAGAAGATTCATAATT+AGG 0.177473 2_4:+18604141 None:intergenic
ATATGAAATGAAAGGAATTT+TGG 0.192707 2_4:+18604767 None:intergenic
TGATTAAACAATAGGTTTAA+TGG 0.202593 2_4:+18601987 None:intergenic
ATAATTGATGTTGCTTCTTC+TGG 0.224592 2_4:-18604294 Msa0319220:CDS
ATAACAGCAGAAGGTTTAAT+TGG 0.226101 2_4:+18604096 None:intergenic
TTGTTCTCTTGTTCTTCTTT+TGG 0.236616 2_4:+18602197 None:intergenic
CTTTGTTTCTTCATCAAGTA+TGG 0.245608 2_4:-18604670 Msa0319220:CDS
GGTGCATGCATTTAAATTCT+AGG 0.261273 2_4:+18601820 None:intergenic
CATCATAGCCTTCTCTTGTT+TGG 0.267430 2_4:+18601852 None:intergenic
GGATAAGGTGAACCTTTAAT+AGG 0.279412 2_4:+18603116 None:intergenic
CAAAACCTTGATTAAACAAT+AGG 0.283013 2_4:+18601979 None:intergenic
CAGTGATGGACTTTCTTATA+AGG 0.284819 2_4:-18602072 Msa0319220:CDS
ACTGAGTCCCACATTCCTTT+TGG 0.291123 2_4:-18601260 Msa0319220:three_prime_UTR
CAATGTGAAGGAAGAGAATA+TGG 0.300905 2_4:-18602138 Msa0319220:CDS
CTATTGTTTAATCAAGGTTT+TGG 0.313071 2_4:-18601978 Msa0319220:CDS
TAATTAGGTAACTTTGGATT+AGG 0.313774 2_4:+18604156 None:intergenic
TTGTGGGCTTGGAGAAAATA+TGG 0.327482 2_4:-18603146 Msa0319220:CDS
TCCTTCACATTGTTAACAAC+TGG 0.339482 2_4:+18602149 None:intergenic
TATGGTCAGAAGCCTATTAA+AGG 0.340677 2_4:-18603128 Msa0319220:CDS
AAGAGTGCTGGTTGGTTGAT+TGG 0.341583 2_4:-18601294 Msa0319220:three_prime_UTR
TCTCTTAAAGTTGAGAATAA+TGG 0.344831 2_4:+18604402 None:intergenic
TTCCTTCAGATTTGTGGGCT+TGG 0.351444 2_4:-18603157 Msa0319220:CDS
ATCCATTTCATCTCTTTGAT+TGG 0.356655 2_4:-18601914 Msa0319220:CDS
TGTTATTATTCATCATGGTT+TGG 0.359561 2_4:+18604729 None:intergenic
AAGGAATGTGGGACTCAGTT+TGG 0.363185 2_4:+18601264 None:intergenic
GCAGAAGGTTTAATTGGTCT+TGG 0.364059 2_4:+18604102 None:intergenic
TTTCATCTCTTTGATTGGTC+AGG 0.365054 2_4:-18601909 Msa0319220:CDS
GACCAATCAAAGAGATGAAA+TGG 0.366081 2_4:+18601912 None:intergenic
GAGTTCAGATCTCATCTTCC+AGG 0.376813 2_4:-18604023 Msa0319220:CDS
TCGTTTAAGACCTGTATTCC+TGG 0.388718 2_4:+18604005 None:intergenic
GATTCATAATTAGGTAACTT+TGG 0.391610 2_4:+18604150 None:intergenic
TAGGTTATAACCAACATGTT+TGG 0.405752 2_4:+18602353 None:intergenic
GTGGTAATTCAAGTAATGAT+AGG 0.407677 2_4:-18601956 Msa0319220:CDS
AAGTAGTTCCTTCAGATTTG+TGG 0.419426 2_4:-18603163 Msa0319220:CDS
ATACTACTCAAGGTCATGTT+TGG 0.422635 2_4:-18601720 Msa0319220:three_prime_UTR
TTCTTGTTATTTACCAGAAA+AGG 0.424080 2_4:-18601350 Msa0319220:three_prime_UTR
GGAACAAACATGAATATGTT+TGG 0.426445 2_4:-18604429 Msa0319220:CDS
GCTAAGGCATTTCTTTGTGT+TGG 0.434825 2_4:+18602305 None:intergenic
AGTAGTTCCTTCAGATTTGT+GGG 0.437348 2_4:-18603162 Msa0319220:CDS
TTAAACCTATTGTTTAATCA+AGG 0.440685 2_4:-18601984 Msa0319220:CDS
ATCTCATCTTCCAGGAATAC+AGG 0.440973 2_4:-18604015 Msa0319220:CDS
TGTCTACTATTTGCAGCTGC+TGG 0.441459 2_4:+18603194 None:intergenic
TTTCTTGTTATTATTCATCA+TGG 0.447837 2_4:+18604724 None:intergenic
CTATTTGCAGCTGCTGGTGC+AGG 0.451080 2_4:+18603200 None:intergenic
ATAATCAGAGGTGGTACTGC+TGG 0.461238 2_4:-18604621 Msa0319220:CDS
CTTAGCATTAATATTGATGT+TGG 0.461905 2_4:+18604068 None:intergenic
AAAGTAACTTACCTTGGATA+AGG 0.462105 2_4:+18603101 None:intergenic
GCTGCAAATAGTAGACAAAC+TGG 0.465679 2_4:-18603188 Msa0319220:CDS
TGGTTCTTCAAATGAGTTGT+TGG 0.473949 2_4:+18601872 None:intergenic
ATGAAATGGATCTAAAACCT+TGG 0.480200 2_4:+18601926 None:intergenic
CCATCCACCAATATTCCAAA+AGG 0.483621 2_4:+18601245 None:intergenic
AGTACTTGTGATGTTGAAGT+AGG 0.496007 2_4:+18604341 None:intergenic
GGTTTAATTGGTCTTGGAGA+AGG 0.498591 2_4:+18604108 None:intergenic
GCAACATCAATTATAGAAGC+AGG 0.500612 2_4:+18604303 None:intergenic
AGATCTTGTTGAACCTGCTA+AGG 0.507584 2_4:+18602289 None:intergenic
TATAGATGCAGCAGTTCAAA+GGG 0.512628 2_4:-18602416 Msa0319220:CDS
CTACAAATTGAGATGGATGA+AGG 0.513528 2_4:-18602101 Msa0319220:CDS
TTATAGATGCAGCAGTTCAA+AGG 0.515496 2_4:-18602417 Msa0319220:CDS
TTGGTTGAAATAACAGCAGA+AGG 0.519861 2_4:+18604087 None:intergenic
TCTGTTCTTGAAGACAATAG+AGG 0.525437 2_4:-18604453 Msa0319220:CDS
CAACATCAATATTAATGCTA+AGG 0.530488 2_4:-18604067 Msa0319220:CDS
ACACAAAGAAATGCCTTAGC+AGG 0.532059 2_4:-18602302 Msa0319220:CDS
GAACAGAAGAGAAACTCAAA+GGG 0.532880 2_4:+18604469 None:intergenic
TGGACTTTCTTATAAGGTGA+TGG 0.535954 2_4:-18602066 Msa0319220:CDS
GCATTTCTTTGTGTTGGCCA+TGG 0.541996 2_4:+18602311 None:intergenic
CTCCAAGCCCACAAATCTGA+AGG 0.549243 2_4:+18603155 None:intergenic
GCTGGAGCATATACTAGTAC+AGG 0.551951 2_4:-18604603 Msa0319220:CDS
CCAATATTCCAAAAGGAATG+TGG 0.553908 2_4:+18601252 None:intergenic
GAGAACAATAATAACAGTGA+TGG 0.557395 2_4:-18602182 Msa0319220:CDS
TCCAGTTGTTAACAATGTGA+AGG 0.558008 2_4:-18602150 Msa0319220:CDS
ATAATGATATTAATTAGAGA+TGG 0.558202 2_4:+18601515 None:intergenic
TTGAGGTAGAAATTGTTGGT+AGG 0.563762 2_4:+18601799 None:intergenic
TTAAAGGTTCACCTTATCCA+AGG 0.571044 2_4:-18603112 Msa0319220:intron
TTGAAGAACCAAACAAGAGA+AGG 0.573435 2_4:-18601860 Msa0319220:CDS
TGGTAATTCAAGTAATGATA+GGG 0.573489 2_4:-18601955 Msa0319220:CDS
AGAACAGAAGAGAAACTCAA+AGG 0.577454 2_4:+18604468 None:intergenic
AGAGAAGCTACAAATTGAGA+TGG 0.578599 2_4:-18602108 Msa0319220:CDS
GCATATATATATGAAATGAA+AGG 0.579416 2_4:+18604759 None:intergenic
CAATATTCCAAAAGGAATGT+GGG 0.593084 2_4:+18601253 None:intergenic
CAGAACTGATCCAAACATGT+TGG 0.596203 2_4:-18602363 Msa0319220:CDS
ATGATTGTGTTCTGAAGTGT+AGG 0.601847 2_4:+18602334 None:intergenic
ACAGAAAGCTGAGGAACAGA+AGG 0.602399 2_4:-18601221 Msa0319220:three_prime_UTR
GGAATACAGGTCTTAAACGA+AGG 0.603168 2_4:-18604002 Msa0319220:intron
ATCATGATCATGATAATCAG+TGG 0.603815 2_4:-18604539 Msa0319220:CDS
GGAAAATAGCAAAATTCATG+AGG 0.604467 2_4:-18602045 Msa0319220:CDS
GTTCAAAGGGATGTTCAGCT+AGG 0.605745 2_4:-18602403 Msa0319220:CDS
TGATGATGATGTGAAACTGC+AGG 0.613240 2_4:+18604558 None:intergenic
GATGAAGGAGAATTCAGTGA+TGG 0.613782 2_4:-18602086 Msa0319220:CDS
TAATGATAGGGATCAATCCA+AGG 0.614402 2_4:-18601943 Msa0319220:CDS
GGTGCTAGCTATACTACTCA+AGG 0.615709 2_4:-18601730 Msa0319220:three_prime_UTR
ATGTTGAAGTAGGAAGAAGA+AGG 0.618243 2_4:+18604351 None:intergenic
CTTCAGAACACAATCATCCA+TGG 0.626675 2_4:-18602328 Msa0319220:CDS
ATTTGAAGTGTGTCATGTGG+AGG 0.628533 2_4:-18601687 Msa0319220:three_prime_UTR
TCAAGTATGGACAATTACCT+AGG 0.636819 2_4:-18604657 Msa0319220:CDS
TTTGAAGTGTGTCATGTGGA+GGG 0.640138 2_4:-18601686 Msa0319220:three_prime_UTR
GTTCTTGAAGACAATAGAGG+AGG 0.649107 2_4:-18604450 Msa0319220:CDS
GATTTAACTGACATAATCAG+AGG 0.660960 2_4:-18604633 Msa0319220:CDS
GTGATTTGAAGTGTGTCATG+TGG 0.673071 2_4:-18601690 Msa0319220:three_prime_UTR
GATTCTCTTCTAGACAACAC+AGG 0.690701 2_4:-18604045 Msa0319220:CDS
TTAACTGACATAATCAGAGG+TGG 0.698541 2_4:-18604630 Msa0319220:CDS
GTGGATGGGACAGAAAGCTG+AGG 0.705531 2_4:-18601230 Msa0319220:three_prime_UTR
AAAAGAGAAAGAGTGCTGGT+TGG 0.706845 2_4:-18601302 Msa0319220:three_prime_UTR
TTATGTCAGTTAAATCACCT+AGG 0.732430 2_4:+18604640 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAAAACTATTAGATT+AGG + chr2_4:18602611-18602630 None:intergenic 10.0%
!!! TGTTTCTTATTATTATTTTT+TGG - chr2_4:18603547-18603566 Msa0319220:intron 10.0%
!! TGAAATTAATTATAGTAACA+TGG + chr2_4:18602122-18602141 None:intergenic 15.0%
!!! ATTTTGAAGTAGAAATTTTA+TGG - chr2_4:18602191-18602210 Msa0319220:CDS 15.0%
!! TGATTTATTCCAATTAAAAT+AGG + chr2_4:18602574-18602593 None:intergenic 15.0%
!!! GTTTTTTTTATTTCCTTTTT+TGG - chr2_4:18602620-18602639 Msa0319220:intron 15.0%
!! ATAATGATATTAATTAGAGA+TGG + chr2_4:18604491-18604510 None:intergenic 15.0%
!! CAAGAAAAATAAATAAAGTA+GGG + chr2_4:18604639-18604658 None:intergenic 15.0%
!! ACAAGAAAAATAAATAAAGT+AGG + chr2_4:18604640-18604659 None:intergenic 15.0%
!! ATAACAAAAAACATGCAATA+AGG + chr2_4:18601205-18601224 None:intergenic 20.0%
!!! ATATGAAATGAAAGGAATTT+TGG + chr2_4:18601239-18601258 None:intergenic 20.0%
!! GCATATATATATGAAATGAA+AGG + chr2_4:18601247-18601266 None:intergenic 20.0%
!!! TTTCTTGTTATTATTCATCA+TGG + chr2_4:18601282-18601301 None:intergenic 20.0%
!!! TTTGAGAATATGTTTTTACA+TGG + chr2_4:18601811-18601830 None:intergenic 20.0%
!!! TTTAAGATAAGTTTCTTGAA+AGG + chr2_4:18602450-18602469 None:intergenic 20.0%
!! TCTAAAAATTAAAAGGACTA+TGG + chr2_4:18602534-18602553 None:intergenic 20.0%
!!! TTGGTAGTCTAAAAATTAAA+AGG + chr2_4:18602541-18602560 None:intergenic 20.0%
!! TTATTCCAATTAAAATAGGT+AGG + chr2_4:18602570-18602589 None:intergenic 20.0%
!! AAAAAAACATGTACCAAAAA+AGG + chr2_4:18602636-18602655 None:intergenic 20.0%
!! TAGCAATTAAAAATACATTG+AGG - chr2_4:18602942-18602961 Msa0319220:intron 20.0%
!! AAGGAAATGATTATAGTTAT+AGG + chr2_4:18603013-18603032 None:intergenic 20.0%
!! TTACAAGTAACAATATACAA+TGG + chr2_4:18603075-18603094 None:intergenic 20.0%
!! GTTTAATTAAGAAGCTTATT+AGG - chr2_4:18603181-18603200 Msa0319220:CDS 20.0%
!!! TGTATTAAGCAATTTTGATT+TGG - chr2_4:18603343-18603362 Msa0319220:intron 20.0%
!!! TTGATTTGGTATATAATGTT+AGG - chr2_4:18603357-18603376 Msa0319220:intron 20.0%
!! AATATTGTTACGAAACTTAA+AGG + chr2_4:18603432-18603451 None:intergenic 20.0%
!!! ATTATTTTTTGGTTGCTATT+AGG - chr2_4:18603558-18603577 Msa0319220:intron 20.0%
!!! TTATTTTTTGGTTGCTATTA+GGG - chr2_4:18603559-18603578 Msa0319220:intron 20.0%
!! TGATTAAACAATAGGTTTAA+TGG + chr2_4:18604019-18604038 None:intergenic 20.0%
!! TTAAACCTATTGTTTAATCA+AGG - chr2_4:18604019-18604038 Msa0319220:CDS 20.0%
!!! TCTTGCTTTTTAGTATTATT+TGG - chr2_4:18604249-18604268 Msa0319220:CDS 20.0%
!! AATCCAAAAAATTGAACTAT+CGG + chr2_4:18604525-18604544 None:intergenic 20.0%
!!! ATAAGGTTTTTTGGCTATTA+AGG + chr2_4:18601188-18601207 None:intergenic 25.0%
!!! AAACATGCAATAAGGTTTTT+TGG + chr2_4:18601197-18601216 None:intergenic 25.0%
! TGTTATTATTCATCATGGTT+TGG + chr2_4:18601277-18601296 None:intergenic 25.0%
! TCTCTTAAAGTTGAGAATAA+TGG + chr2_4:18601604-18601623 None:intergenic 25.0%
! TGATCAAGTTCTTGTAAAAA+TGG + chr2_4:18601628-18601647 None:intergenic 25.0%
! TAATTAGGTAACTTTGGATT+AGG + chr2_4:18601850-18601869 None:intergenic 25.0%
! GATTCATAATTAGGTAACTT+TGG + chr2_4:18601856-18601875 None:intergenic 25.0%
! GTTGAAGAAGATTCATAATT+AGG + chr2_4:18601865-18601884 None:intergenic 25.0%
! CTTAGCATTAATATTGATGT+TGG + chr2_4:18601938-18601957 None:intergenic 25.0%
! CAACATCAATATTAATGCTA+AGG - chr2_4:18601936-18601955 Msa0319220:CDS 25.0%
!! TAAAATAGGTAGGTATGTTT+TGG + chr2_4:18602560-18602579 None:intergenic 25.0%
!!! ACATACCTACCTATTTTAAT+TGG - chr2_4:18602562-18602581 Msa0319220:intron 25.0%
! TTGTGTAATGAATTAATTGC+AGG - chr2_4:18602750-18602769 Msa0319220:intron 25.0%
! AGATAACAAAAACAACAACA+AGG - chr2_4:18603102-18603121 Msa0319220:intron 25.0%
! TTAGAGAGAGATTTCTTAAT+TGG + chr2_4:18603138-18603157 None:intergenic 25.0%
!!! CTTTTCATATTCATTGATTG+AGG - chr2_4:18603234-18603253 Msa0319220:CDS 25.0%
!!! TATTTTTTGGTTGCTATTAG+GGG - chr2_4:18603560-18603579 Msa0319220:intron 25.0%
!!! CTTTGTAGAAGTATTGTTTT+TGG + chr2_4:18603747-18603766 None:intergenic 25.0%
! CAAAACCTTGATTAAACAAT+AGG + chr2_4:18604027-18604046 None:intergenic 25.0%
!! CTATTGTTTAATCAAGGTTT+TGG - chr2_4:18604025-18604044 Msa0319220:CDS 25.0%
! TGGTAATTCAAGTAATGATA+GGG - chr2_4:18604048-18604067 Msa0319220:CDS 25.0%
!!! TTTTTTGAGGTAGAAATTGT+TGG + chr2_4:18604211-18604230 None:intergenic 25.0%
!!! AACTAGGTCTTTTTTTTTTG+AGG + chr2_4:18604224-18604243 None:intergenic 25.0%
!! AAAAAGCAAGATTGATAACT+AGG + chr2_4:18604240-18604259 None:intergenic 25.0%
!!! TGCTTTTTAGTATTATTTGG+TGG - chr2_4:18604252-18604271 Msa0319220:CDS 25.0%
!!! TTTCCGATAGTTCAATTTTT+TGG - chr2_4:18604519-18604538 Msa0319220:CDS 25.0%
!!! TTCTTGTTATTTACCAGAAA+AGG - chr2_4:18604653-18604672 Msa0319220:CDS 25.0%
AGCAAGAAAAAGCAAGTTTA+GGG + chr2_4:18601165-18601184 None:intergenic 30.0%
! CTTTGTTTCTTCATCAAGTA+TGG - chr2_4:18601333-18601352 Msa0319220:three_prime_UTR 30.0%
TTATGTCAGTTAAATCACCT+AGG + chr2_4:18601366-18601385 None:intergenic 30.0%
GATTTAACTGACATAATCAG+AGG - chr2_4:18601370-18601389 Msa0319220:three_prime_UTR 30.0%
ATCATGATCATGATAATCAG+TGG - chr2_4:18601464-18601483 Msa0319220:three_prime_UTR 30.0%
GGAACAAACATGAATATGTT+TGG - chr2_4:18601574-18601593 Msa0319220:three_prime_UTR 30.0%
! ATAATTGATGTTGCTTCTTC+TGG - chr2_4:18601709-18601728 Msa0319220:three_prime_UTR 30.0%
ATAACAGCAGAAGGTTTAAT+TGG + chr2_4:18601910-18601929 None:intergenic 30.0%
AAGTACTATTGAGAGAAAGT+AGG - chr2_4:18602225-18602244 Msa0319220:CDS 30.0%
AGTACTATTGAGAGAAAGTA+GGG - chr2_4:18602226-18602245 Msa0319220:CDS 30.0%
GTTATCAAGACTATAACATG+TGG - chr2_4:18602327-18602346 Msa0319220:CDS 30.0%
! CATGTGGTTTAATTTCTTTG+AGG - chr2_4:18602343-18602362 Msa0319220:CDS 30.0%
!!! TAGGAATTTTCAGGTAGTTA+TGG + chr2_4:18602485-18602504 None:intergenic 30.0%
! TCAAAGGAATAGGAATTTTC+AGG + chr2_4:18602494-18602513 None:intergenic 30.0%
! TTTTTGTGCATGATGATCAA+AGG + chr2_4:18602510-18602529 None:intergenic 30.0%
! TGAAGAGTGAAGTATTTGAT+TGG - chr2_4:18602698-18602717 Msa0319220:intron 30.0%
AAGTATTTGATTGGTGGAAA+TGG - chr2_4:18602707-18602726 Msa0319220:intron 30.0%
AAAGTAACTTACCTTGGATA+AGG + chr2_4:18602905-18602924 None:intergenic 30.0%
TCTAAGAAAGTAACTTACCT+TGG + chr2_4:18602911-18602930 None:intergenic 30.0%
AAACACAGTAGTACATTACA+AGG + chr2_4:18603032-18603051 None:intergenic 30.0%
TAAAGGACTAATCTGATACA+GGG + chr2_4:18603415-18603434 None:intergenic 30.0%
TTAAAGGACTAATCTGATAC+AGG + chr2_4:18603416-18603435 None:intergenic 30.0%
TTCGTAACAATATTTCCGTT+AGG - chr2_4:18603438-18603457 Msa0319220:intron 30.0%
GAAACAATGTCAAAACCTAA+CGG + chr2_4:18603456-18603475 None:intergenic 30.0%
!! GTTTTGACATTGTTTCAGAT+TGG - chr2_4:18603460-18603479 Msa0319220:intron 30.0%
AATACCTAAACGAGACTTAA+AGG + chr2_4:18603487-18603506 None:intergenic 30.0%
TAGGTTATAACCAACATGTT+TGG + chr2_4:18603653-18603672 None:intergenic 30.0%
!!! GTAGAAGTATTGTTTTTGGA+TGG + chr2_4:18603743-18603762 None:intergenic 30.0%
! TTGTTCTCTTGTTCTTCTTT+TGG + chr2_4:18603809-18603828 None:intergenic 30.0%
GAGAACAATAATAACAGTGA+TGG - chr2_4:18603821-18603840 Msa0319220:intron 30.0%
GGAAAATAGCAAAATTCATG+AGG - chr2_4:18603958-18603977 Msa0319220:intron 30.0%
!! TTGTTTAATCAAGGTTTTGG+TGG - chr2_4:18604028-18604047 Msa0319220:CDS 30.0%
GTGGTAATTCAAGTAATGAT+AGG - chr2_4:18604047-18604066 Msa0319220:CDS 30.0%
! ATGAAATGGATCTAAAACCT+TGG + chr2_4:18604080-18604099 None:intergenic 30.0%
ATCCATTTCATCTCTTTGAT+TGG - chr2_4:18604089-18604108 Msa0319220:CDS 30.0%
!!! AATTTTTTCACTGCCTTTTC+TGG + chr2_4:18604669-18604688 None:intergenic 30.0%
CAATATTCCAAAAGGAATGT+GGG + chr2_4:18604753-18604772 None:intergenic 30.0%
! AGTTTAGGGAACAGGTTTTT+GGG + chr2_4:18601151-18601170 None:intergenic 35.0%
! AAGTTTAGGGAACAGGTTTT+TGG + chr2_4:18601152-18601171 None:intergenic 35.0%
AAAAAGCAAGTTTAGGGAAC+AGG + chr2_4:18601159-18601178 None:intergenic 35.0%
GAGCAAGAAAAAGCAAGTTT+AGG + chr2_4:18601166-18601185 None:intergenic 35.0%
TCAAGTATGGACAATTACCT+AGG - chr2_4:18601346-18601365 Msa0319220:three_prime_UTR 35.0%
TTAACTGACATAATCAGAGG+TGG - chr2_4:18601373-18601392 Msa0319220:three_prime_UTR 35.0%
GAACAGAAGAGAAACTCAAA+GGG + chr2_4:18601537-18601556 None:intergenic 35.0%
AGAACAGAAGAGAAACTCAA+AGG + chr2_4:18601538-18601557 None:intergenic 35.0%
TCTGTTCTTGAAGACAATAG+AGG - chr2_4:18601550-18601569 Msa0319220:three_prime_UTR 35.0%
ATGTTGAAGTAGGAAGAAGA+AGG + chr2_4:18601655-18601674 None:intergenic 35.0%
AGTACTTGTGATGTTGAAGT+AGG + chr2_4:18601665-18601684 None:intergenic 35.0%
GCAACATCAATTATAGAAGC+AGG + chr2_4:18601703-18601722 None:intergenic 35.0%
TTGGTTGAAATAACAGCAGA+AGG + chr2_4:18601919-18601938 None:intergenic 35.0%
TATTGAGAGAAAGTAGGGTT+TGG - chr2_4:18602231-18602250 Msa0319220:CDS 35.0%
AGTAGGGTTTGGCATAAATT+AGG - chr2_4:18602242-18602261 Msa0319220:CDS 35.0%
!! AGAAGCTAACTCTACTTTTC+AGG + chr2_4:18602390-18602409 None:intergenic 35.0%
TGCATGATGATCAAAGGAAT+AGG + chr2_4:18602504-18602523 None:intergenic 35.0%
! AGAGTGAAGTATTTGATTGG+TGG - chr2_4:18602701-18602720 Msa0319220:intron 35.0%
CAGGAAGAATCAAGCAAAAA+AGG - chr2_4:18602769-18602788 Msa0319220:intron 35.0%
GAAGAATCAAGCAAAAAAGG+TGG - chr2_4:18602772-18602791 Msa0319220:intron 35.0%
AAGTAGTTCCTTCAGATTTG+TGG - chr2_4:18602840-18602859 Msa0319220:intron 35.0%
AGTAGTTCCTTCAGATTTGT+GGG - chr2_4:18602841-18602860 Msa0319220:intron 35.0%
TATGGTCAGAAGCCTATTAA+AGG - chr2_4:18602875-18602894 Msa0319220:intron 35.0%
GGATAAGGTGAACCTTTAAT+AGG + chr2_4:18602890-18602909 None:intergenic 35.0%
TTAAAGGTTCACCTTATCCA+AGG - chr2_4:18602891-18602910 Msa0319220:intron 35.0%
CAATGGTCAAGCTAAAAATG+TGG + chr2_4:18603058-18603077 None:intergenic 35.0%
CAAAAACAACAACAAGGCTT+AGG - chr2_4:18603108-18603127 Msa0319220:intron 35.0%
TTATAGATGCAGCAGTTCAA+AGG - chr2_4:18603586-18603605 Msa0319220:intron 35.0%
TATAGATGCAGCAGTTCAAA+GGG - chr2_4:18603587-18603606 Msa0319220:intron 35.0%
!! ATGATTGTGTTCTGAAGTGT+AGG + chr2_4:18603672-18603691 None:intergenic 35.0%
TCCAGTTGTTAACAATGTGA+AGG - chr2_4:18603853-18603872 Msa0319220:intron 35.0%
TCCTTCACATTGTTAACAAC+TGG + chr2_4:18603857-18603876 None:intergenic 35.0%
CAATGTGAAGGAAGAGAATA+TGG - chr2_4:18603865-18603884 Msa0319220:intron 35.0%
AGAGAAGCTACAAATTGAGA+TGG - chr2_4:18603895-18603914 Msa0319220:intron 35.0%
CTACAAATTGAGATGGATGA+AGG - chr2_4:18603902-18603921 Msa0319220:intron 35.0%
!! CAGTGATGGACTTTCTTATA+AGG - chr2_4:18603931-18603950 Msa0319220:intron 35.0%
TGGACTTTCTTATAAGGTGA+TGG - chr2_4:18603937-18603956 Msa0319220:intron 35.0%
!!! TGAGGACTTTTTTGCTGAAT+TGG - chr2_4:18603976-18603995 Msa0319220:intron 35.0%
!!! GAGGACTTTTTTGCTGAATT+GGG - chr2_4:18603977-18603996 Msa0319220:intron 35.0%
TAATGATAGGGATCAATCCA+AGG - chr2_4:18604060-18604079 Msa0319220:CDS 35.0%
GACCAATCAAAGAGATGAAA+TGG + chr2_4:18604094-18604113 None:intergenic 35.0%
! TTTCATCTCTTTGATTGGTC+AGG - chr2_4:18604094-18604113 Msa0319220:CDS 35.0%
TGGTTCTTCAAATGAGTTGT+TGG + chr2_4:18604134-18604153 None:intergenic 35.0%
TTGAAGAACCAAACAAGAGA+AGG - chr2_4:18604143-18604162 Msa0319220:CDS 35.0%
GGTGCATGCATTTAAATTCT+AGG + chr2_4:18604186-18604205 None:intergenic 35.0%
TTGAGGTAGAAATTGTTGGT+AGG + chr2_4:18604207-18604226 None:intergenic 35.0%
ATACTACTCAAGGTCATGTT+TGG - chr2_4:18604283-18604302 Msa0319220:CDS 35.0%
CCAATATTCCAAAAGGAATG+TGG + chr2_4:18604754-18604773 None:intergenic 35.0%
!!! CCACATTCCTTTTGGAATAT+TGG - chr2_4:18604751-18604770 Msa0319220:five_prime_UTR 35.0%
!!! CATTCCTTTTGGAATATTGG+TGG - chr2_4:18604754-18604773 Msa0319220:five_prime_UTR 35.0%
!!! CTTTTGGAATATTGGTGGAT+GGG - chr2_4:18604759-18604778 Msa0319220:five_prime_UTR 35.0%
!! TTTAGGGAACAGGTTTTTGG+GGG + chr2_4:18601149-18601168 None:intergenic 40.0%
! GTTTAGGGAACAGGTTTTTG+GGG + chr2_4:18601150-18601169 None:intergenic 40.0%
! TGATGATGATGTGAAACTGC+AGG + chr2_4:18601448-18601467 None:intergenic 40.0%
GTTCTTGAAGACAATAGAGG+AGG - chr2_4:18601553-18601572 Msa0319220:three_prime_UTR 40.0%
!! GGTTTAATTGGTCTTGGAGA+AGG + chr2_4:18601898-18601917 None:intergenic 40.0%
! GCAGAAGGTTTAATTGGTCT+TGG + chr2_4:18601904-18601923 None:intergenic 40.0%
GATTCTCTTCTAGACAACAC+AGG - chr2_4:18601958-18601977 Msa0319220:CDS 40.0%
ATCTCATCTTCCAGGAATAC+AGG - chr2_4:18601988-18602007 Msa0319220:CDS 40.0%
! TCGTTTAAGACCTGTATTCC+TGG + chr2_4:18602001-18602020 None:intergenic 40.0%
GGAATACAGGTCTTAAACGA+AGG - chr2_4:18602001-18602020 Msa0319220:CDS 40.0%
! CTTTTTCTTGTGACTACACG+TGG - chr2_4:18602275-18602294 Msa0319220:CDS 40.0%
ACTACACGTGGCACTATTAA+TGG - chr2_4:18602287-18602306 Msa0319220:CDS 40.0%
GCTGCAAATAGTAGACAAAC+TGG - chr2_4:18602815-18602834 Msa0319220:intron 40.0%
TTGTGGGCTTGGAGAAAATA+TGG - chr2_4:18602857-18602876 Msa0319220:intron 40.0%
GATACAGGGTCGAAACTTAA+AGG + chr2_4:18603401-18603420 None:intergenic 40.0%
TGGTCCTTTAAGTCTCGTTT+AGG - chr2_4:18603480-18603499 Msa0319220:intron 40.0%
CAGAACTGATCCAAACATGT+TGG - chr2_4:18603640-18603659 Msa0319220:intron 40.0%
CTTCAGAACACAATCATCCA+TGG - chr2_4:18603675-18603694 Msa0319220:intron 40.0%
! GCTAAGGCATTTCTTTGTGT+TGG + chr2_4:18603701-18603720 None:intergenic 40.0%
ACACAAAGAAATGCCTTAGC+AGG - chr2_4:18603701-18603720 Msa0319220:intron 40.0%
AGATCTTGTTGAACCTGCTA+AGG + chr2_4:18603717-18603736 None:intergenic 40.0%
!!! GGCTTTGTTGTGTCATTTTG+TGG + chr2_4:18603788-18603807 None:intergenic 40.0%
GATGAAGGAGAATTCAGTGA+TGG - chr2_4:18603917-18603936 Msa0319220:intron 40.0%
CATCATAGCCTTCTCTTGTT+TGG + chr2_4:18604154-18604173 None:intergenic 40.0%
! GTGATTTGAAGTGTGTCATG+TGG - chr2_4:18604313-18604332 Msa0319220:CDS 40.0%
! ATTTGAAGTGTGTCATGTGG+AGG - chr2_4:18604316-18604335 Msa0319220:CDS 40.0%
! TTTGAAGTGTGTCATGTGGA+GGG - chr2_4:18604317-18604336 Msa0319220:CDS 40.0%
! GTGAAAAAGAGAAAGAGTGC+TGG - chr2_4:18604697-18604716 Msa0319220:exon 40.0%
!! AAAAGAGAAAGAGTGCTGGT+TGG - chr2_4:18604701-18604720 Msa0319220:exon 40.0%
CCATCCACCAATATTCCAAA+AGG + chr2_4:18604761-18604780 None:intergenic 40.0%
!!! CCTTTTGGAATATTGGTGGA+TGG - chr2_4:18604758-18604777 Msa0319220:five_prime_UTR 40.0%
ATAATCAGAGGTGGTACTGC+TGG - chr2_4:18601382-18601401 Msa0319220:three_prime_UTR 45.0%
GCTGGAGCATATACTAGTAC+AGG - chr2_4:18601400-18601419 Msa0319220:three_prime_UTR 45.0%
GAGTTCAGATCTCATCTTCC+AGG - chr2_4:18601980-18601999 Msa0319220:CDS 45.0%
TGTCTACTATTTGCAGCTGC+TGG + chr2_4:18602812-18602831 None:intergenic 45.0%
TTCCTTCAGATTTGTGGGCT+TGG - chr2_4:18602846-18602865 Msa0319220:intron 45.0%
GTTCAAAGGGATGTTCAGCT+AGG - chr2_4:18603600-18603619 Msa0319220:intron 45.0%
! GCATTTCTTTGTGTTGGCCA+TGG + chr2_4:18603695-18603714 None:intergenic 45.0%
GGTGCTAGCTATACTACTCA+AGG - chr2_4:18604273-18604292 Msa0319220:CDS 45.0%
!! AAGAGTGCTGGTTGGTTGAT+TGG - chr2_4:18604709-18604728 Msa0319220:five_prime_UTR 45.0%
AAGGAATGTGGGACTCAGTT+TGG + chr2_4:18604742-18604761 None:intergenic 45.0%
! ACTGAGTCCCACATTCCTTT+TGG - chr2_4:18604743-18604762 Msa0319220:five_prime_UTR 45.0%
ACAGAAAGCTGAGGAACAGA+AGG - chr2_4:18604782-18604801 Msa0319220:five_prime_UTR 45.0%
CTCCAAGCCCACAAATCTGA+AGG + chr2_4:18602851-18602870 None:intergenic 50.0%
!! GGGAACAGGTTTTTGGGGGT+TGG + chr2_4:18601145-18601164 None:intergenic 55.0%
CTATTTGCAGCTGCTGGTGC+AGG + chr2_4:18602806-18602825 None:intergenic 55.0%
GTGGATGGGACAGAAAGCTG+AGG - chr2_4:18604773-18604792 Msa0319220:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 18601135 18604890 18601135 ID=Msa0319220;Name=Msa0319220
chr2_4 mRNA 18601135 18604890 18601135 ID=Msa0319220-mRNA-1;Parent=Msa0319220;Name=Msa0319220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=187|1|1|1|1|1|3|719|477
chr2_4 exon 18601135 18602445 18601135 ID=Msa0319220-mRNA-1:exon:6953;Parent=Msa0319220-mRNA-1
chr2_4 exon 18603113 18603253 18603113 ID=Msa0319220-mRNA-1:exon:6952;Parent=Msa0319220-mRNA-1
chr2_4 exon 18604003 18604890 18604003 ID=Msa0319220-mRNA-1:exon:6951;Parent=Msa0319220-mRNA-1
chr2_4 five_prime_UTR 18604704 18604890 18604704 ID=Msa0319220-mRNA-1:five_prime_utr;Parent=Msa0319220-mRNA-1
chr2_4 CDS 18604003 18604703 18604003 ID=Msa0319220-mRNA-1:cds;Parent=Msa0319220-mRNA-1
chr2_4 CDS 18603113 18603253 18603113 ID=Msa0319220-mRNA-1:cds;Parent=Msa0319220-mRNA-1
chr2_4 CDS 18601854 18602445 18601854 ID=Msa0319220-mRNA-1:cds;Parent=Msa0319220-mRNA-1
chr2_4 three_prime_UTR 18601135 18601853 18601135 ID=Msa0319220-mRNA-1:three_prime_utr;Parent=Msa0319220-mRNA-1
Gene Sequence

>Msa0319220

ATGTGTAGTATCTTTGTTTCTTCATCAAGTATGGACAATTACCTAGGTGATTTAACTGACATAATCAGAGGTGGTACTGCTGGAGCATATACTAGTACAGGAACTTCATCATCATCTGCTGAACCTGCAGTTTCACATCATCATCATGATCATGATAATCAGTGGAATCATCATCATCATAATCATCAATTCTCTTCTGATCATCATCATCACCCTTTGAGTTTCTCTTCTGTTCTTGAAGACAATAGAGGAGGAACAAACATGAATATGTTTGGTGATCCATTATTCTCAACTTTAAGAGATCCATTTTTACAAGAACTTGATCATATACCTTCTTCTTCCTACTTCAACATCACAAGTACTACTAGTACTACTTCACCTGCTTCTATAATTGATGTTGCTTCTTCTGGTGTTAGTGTTACTACTTCATCATCTTCTTCTGCTTCTGTTTTTGCACTTGATCATGAGATGAGATCAATATCAAGACCATGTAAAAACATATTCTCAAATATGATTCAGATCTCTCCTAATCCAAAGTTACCTAATTATGAATCTTCTTCAACAATAGCAGCACCTTCTCCAAGACCAATTAAACCTTCTGCTGTTATTTCAACCAACATCAATATTAATGCTAAGGATTCTCTTCTAGACAACACAGGAGTTCAGATCTCATCTTCCAGGAATACAGGTCTTAAACGAAGGAAGAATCAAGCAAAAAAGGTGGTTTGTATTCCTGCACCAGCAGCTGCAAATAGTAGACAAACTGGAGAAGTAGTTCCTTCAGATTTGTGGGCTTGGAGAAAATATGGTCAGAAGCCTATTAAAGGTTCACCTTATCCAAGGGGTTATTATAGATGCAGCAGTTCAAAGGGATGTTCAGCTAGGAAACAAGTTGAGAGAAGCAGAACTGATCCAAACATGTTGGTTATAACCTACACTTCAGAACACAATCATCCATGGCCAACACAAAGAAATGCCTTAGCAGGTTCAACAAGATCTCAACCATCCAAAAACAATACTTCTACAAAGAATTCAGAAACATCACAGCCACAAAATGACACAACAAAGCCAAAAGAAGAACAAGAGAACAATAATAACAGTGATGGAAATGATTCTCCAGTTGTTAACAATGTGAAGGAAGAGAATATGGAAGATATAGAGAAGCTACAAATTGAGATGGATGAAGGAGAATTCAGTGATGGACTTTCTTATAAGGTGATGGAAAATAGCAAAATTCATGAGGACTTTTTTGCTGAATTGGGTGAAATTGAAACTGATCCATTAAACCTATTGTTTAATCAAGGTTTTGGTGGTAATTCAAGTAATGATAGGGATCAATCCAAGGTTTTAGATCCATTTCATCTCTTTGATTGGTCAGGTGATAACAACAATACCAACAACTCATTTGAAGAACCAAACAAGAGAAGGCTATGA
Protein sequence

>Msa0319220

MCSIFVSSSSMDNYLGDLTDIIRGGTAGAYTSTGTSSSSAEPAVSHHHHDHDNQWNHHHHNHQFSSDHHHHPLSFSSVLEDNRGGTNMNMFGDPLFSTLRDPFLQELDHIPSSSYFNITSTTSTTSPASIIDVASSGVSVTTSSSSSASVFALDHEMRSISRPCKNIFSNMIQISPNPKLPNYESSSTIAAPSPRPIKPSAVISTNININAKDSLLDNTGVQISSSRNTGLKRRKNQAKKVVCIPAPAAANSRQTGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSQPSKNNTSTKNSETSQPQNDTTKPKEEQENNNNSDGNDSPVVNNVKEENMEDIEKLQIEMDEGEFSDGLSYKVMENSKIHEDFFAELGEIETDPLNLLFNQGFGGNSSNDRDQSKVLDPFHLFDWSGDNNNTNNSFEEPNKRRL*